BrabA.00111.a

Glutamine synthetase class-I, adenylation site:Glutamine synthetase type I:Glutamine synthetase, catalytic domain:Glutamine s...

CENTER ID: BrabA.00111.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: diffraction
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BrabA.00111.a.A2.GE28060 Created from Decode construct for feature BrabA.00111.a 1 467
BrabA.00111.a.A3.GE28061 Created from Decode construct for feature BrabA.00111.a 2 469
BrabA.00111.a.A4.GE28062 Created from Decode construct for feature BrabA.00111.a 2 467
BrabA.00111.a.A5.GE28063 Created from Decode construct for feature BrabA.00111.a 15 469
BrabA.00111.a.A6.GE28064 Created from Decode construct for feature BrabA.00111.a 15 467
BrabA.00111.a.B4.GE43038 Created from Decode construct for feature BrabA.00111.a 2 467
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BrabA.00111.a.A1.PS00110 Full length( BrabA.00111.a ) 1 469
BrabA.00111.a.A1.PW25440 Full length( BrabA.00111.a ) 1 469
BrabA.00111.a.A3.PW28709 Created from Decode construct for feature BrabA.00111.a 2 469

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_127086
PATRIC ID: fig|359391.4.peg.1067
RefSeq: YP_414429
UniProt: Q2YQ67

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTANDILKQ IKDNDVKFVD LRFTDPKGKL HHVTMDIGLV DEDMFVDGVM FDGSSIAGWK AINESDMVLM PDPETAHIDP FFAQSTLVIL CDILEPLSGE SYSRDPRTTA KKAEAYLKSL GIGDTVYVGP EAEFFVFDDV KFKADPFNTG FKLDSAELPS NDDTDYETGN LGHRPRVKGG YFPVPPIDSL QDMRSEMLTV LSEMGVTVEK HHHEVASAQH ELGTKFDTLV RNADKMQIQK YVVHQVANAY GKTATFMPKP VFGDNGSGMH VHFSIWKDGK PTFAGNEYAG LSENCLYFIG GVIKHAKAVN AFTNPSTNSY KRLVPGYEAP VLLAYSARNR SASCRIPFGS SPKSKRLEVR FPDPSANPYL CFSALLMAGL DGIKNKIHPG QAMDKDLYDL PPKELKQIPT VCGSLREALQ SLNKDREFLK AGGVFSDDQI DSFIELKMAE VMRFEMTPHP VEFDMYYSV
NT Sequence
ATGACGACTG CCAACGATAT TCTGAAACAG ATCAAAGATA ACGACGTCAA GTTCGTCGAC CTTCGCTTCA CCGATCCGAA GGGCAAGCTC CATCACGTTA CGATGGATAT CGGCCTCGTG GACGAAGACA TGTTCGTCGA CGGTGTCATG TTCGACGGCT CTTCGATCGC AGGCTGGAAG GCCATCAACG AATCCGACAT GGTTTTGATG CCCGATCCGG AAACGGCTCA TATCGACCCG TTCTTCGCCC AGTCCACGCT TGTCATTCTC TGCGATATCC TTGAGCCGCT TTCCGGCGAA AGCTACAGCC GTGATCCGCG TACGACTGCC AAGAAGGCCG AAGCCTATCT GAAATCGCTC GGCATTGGCG ATACGGTCTA TGTCGGCCCG GAAGCCGAAT TCTTCGTCTT CGACGATGTG AAGTTCAAGG CCGATCCGTT CAATACGGGC TTCAAGCTCG ATAGCGCCGA ATTGCCATCC AACGACGACA CCGATTATGA AACCGGCAAT CTCGGCCATC GTCCGCGCGT CAAGGGCGGC TATTTCCCCG TTCCGCCAAT CGACAGCTTG CAGGACATGC GTTCCGAAAT GCTGACCGTG CTCTCGGAAA TGGGTGTGAC GGTTGAAAAG CACCACCATG AAGTCGCTTC CGCGCAGCAC GAACTGGGCA CGAAATTCGA CACGCTGGTG CGCAACGCCG ACAAGATGCA GATTCAGAAA TATGTCGTGC ATCAGGTTGC CAATGCCTAT GGCAAAACGG CAACCTTCAT GCCGAAGCCG GTCTTTGGCG ACAATGGCTC CGGCATGCAT GTTCACTTCT CGATCTGGAA AGACGGCAAG CCGACCTTCG CAGGCAATGA ATATGCTGGT CTGTCGGAAA ACTGCCTCTA CTTCATTGGC GGCGTCATCA AGCACGCAAA AGCTGTGAAT GCCTTCACCA ACCCGTCCAC CAACTCCTAC AAGCGTCTGG TGCCGGGCTA TGAAGCGCCT GTTCTGCTGG CCTATTCCGC ACGCAACCGT TCGGCTTCCT GCCGCATTCC ATTCGGCTCC TCGCCGAAAT CGAAGCGTCT CGAAGTCCGC TTCCCGGATC CGTCCGCCAA CCCTTATCTC TGCTTCTCGG CCCTTCTGAT GGCGGGCCTT GACGGCATCA AGAACAAGAT CCATCCGGGC CAGGCCATGG ACAAGGATCT CTACGACCTG CCGCCGAAGG AACTGAAGCA GATTCCGACC GTCTGCGGCT CCCTGCGTGA AGCTCTACAG TCGCTCAACA AGGATCGTGA ATTCCTCAAG GCTGGCGGCG TGTTCTCCGA CGACCAGATC GACAGCTTCA TCGAGCTGAA AATGGCTGAA GTGATGCGTT TTGAAATGAC GCCGCACCCG GTCGAGTTCG ATATGTATTA CTCGGTA
Details for BrabA.00111.a.A2.GE28060
HARVESTED ON: 12/7/2009
SEQUENCED ON: 12/23/2009
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTTANDILK QIKDNDVKFV DLRFTDPKGK LHHVTMDIGL VDEDMFVDGV MFDGSSIAGW KAINESDMVL MPDPETAHID PFFAQSTLVI LCDILEPLSG ESYSRDPRTT AKKAEAYLKS LGIGDTVYVG PEAEFFVFDD VKFKADPFNT GFKLDSAELP SNDDTDYETG NLGHRPRVKG GYFPVPPIDS LQDMRSEMLT VLSEMGVTVE KHHHEVASAQ HELGTKFDTL VRNADKMQIQ KYVVHQVANA YGKTATFMPK PVFGDNGSGM HVHFSIWKDG KPTFAGNEYA GLSENCLYFI GGVIKHAKAV NAFTNPSTNS YKRLVPGYEA PVLLAYSARN RSASCRIPFG SSPKSKRLEV RFPDPSANPY LCFSALLMAG LDGIKNKIHP GQAMDKDLYD LPPKELKQIP TVCGSLREAL QSLNKDREFL KAGGVFSDDQ IDSFIELKMA EVMRFEMTPH PVEFDMYY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgactgcc aacgatattc tgaaacagat caaagataac gacgtcaagt tcgtcgacct tcgcttcacc gatccgaagg gcaagctcca tcacgttacg atggatatcg gcctcgtgga cgaagacatg ttcgtcgacg gtgtcatgtt cgacggctct tcgatcgcag gctggaaggc catcaacgaa tccgacatgg ttttgatgcc cgatccggaa acggctcata tcgacccgtt cttcgcccag tccacgcttg tcattctctg cgatatcctt gagccgcttt ccggcgaaag ctacagccgt gatccgcgta cgactgccaa gaaggccgaa gcctatctga aatcgctcgg cattggcgat acggtctatg tcggcccgga agccgaattc ttcgtcttcg acgatgtgaa gttcaaggcc gatccgttca atacgggctt caagctcgat agcgccgaat tgccatccaa cgacgacacc gattatgaaa ccggcaatct cggccatcgt ccgcgcgtca agggcggcta tttccccgtt ccgccaatcg acagcttgca ggacatgcgt tccgaaatgc tgaccgtgct ctcggaaatg ggtgtgacgg ttgaaaagca ccaccatgaa gtcgcttccg cgcagcacga actgggcacg aaattcgaca cgctggtgcg caacgccgac aagatgcaga ttcagaaata tgtcgtgcat caggttgcca atgcctatgg caaaacggca accttcatgc cgaagccggt ctttggcgac aatggctccg gcatgcatgt tcacttctcg atctggaaag acggcaagcc gaccttcgca ggcaatgaat atgctggtct gtcggaaaac tgcctctact tcattggcgg cgtcatcaag cacgcaaaag ctgtgaatgc cttcaccaac ccgtccacca actcctacaa gcgtctggtg ccgggctatg aagcgcctgt tctgctggcc tattccgcac gcaaccgttc ggcttcctgc cgcattccat tcggctcctc gccgaaatcg aagcgtctcg aagtccgctt cccggatccg tccgccaacc cttatctctg cttctcggcc cttctgatgg cgggccttga cggcatcaag aacaagatcc atccgggcca ggccatggac aaggatctct acgacctgcc gccgaaggaa ctgaagcaga ttccgaccgt ctgcggctcc ctgcgtgaag ctctacagtc gctcaacaag gatcgtgaat tcctcaaggc tggcggcgtg ttctccgacg accagatcga cagcttcatc gagctgaaaa tggctgaagt gatgcgtttt gaaatgacgc cgcacccggt cgagttcgat atgtattaca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for BrabA.00111.a.A3.GE28061
HARVESTED ON: 12/7/2009
SEQUENCED ON: 12/23/2009
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTTANDILK QIKDNDVKFV DLRFTDPKGK LHHVTMDIGL VDEDMFVDGV MFDGSSIAGW KAINESDMVL MPDPETAHID PFFAQSTLVI LCDILEPLSG ESYSRDPRTT AKKAEAYLKS LGIGDTVYVG PEAEFFVFDD VKFKADPFNT GFKLDSAELP SNDDTDYETG NLGHRPRVKG GYFPVPPIDS LQDMRSEMLT VLSEMGVTVE KHHHEVASAQ HELGTKFDTL VRNADKMQIQ KYVVHQVANA YGKTATFMPK PVFGDNGSGM HVHFSIWKDG KPTFAGNEYA GLSENCLYFI GGVIKHAKAV NAFTNPSTNS YKRLVPGYEA PVLLAYSARN RSASCRIPFG SSPKSKRLEV RFPDPSANPY LCFSALLMAG LDGIKNKIHP GQAMDKDLYD LPPKELKQIP TVCGSLREAL QSLNKDREFL KAGGVFSDDQ IDSFIELKMA EVMRFEMTPH PVEFDMYYSV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgactgcc aacgatattc tgaaacagat caaagataac gacgtcaagt tcgtcgacct tcgcttcacc gatccgaagg gcaagctcca tcacgttacg atggatatcg gcctcgtgga cgaagacatg ttcgtcgacg gtgtcatgtt cgacggctct tcgatcgcag gctggaaggc catcaacgaa tccgacatgg ttttgatgcc cgatccggaa acggctcata tcgacccgtt cttcgcccag tccacgcttg tcattctctg cgatatcctt gagccgcttt ccggcgaaag ctacagccgt gatccgcgta cgactgccaa gaaggccgaa gcctatctga aatcgctcgg cattggcgat acggtctatg tcggcccgga agccgaattc ttcgtcttcg acgatgtgaa gttcaaggcc gatccgttca atacgggctt caagctcgat agcgccgaat tgccatccaa cgacgacacc gattatgaaa ccggcaatct cggccatcgt ccgcgcgtca agggcggcta tttccccgtt ccgccaatcg acagcttgca ggacatgcgt tccgaaatgc tgaccgtgct ctcggaaatg ggtgtgacgg ttgaaaagca ccaccatgaa gtcgcttccg cgcagcacga actgggcacg aaattcgaca cgctggtgcg caacgccgac aagatgcaga ttcagaaata tgtcgtgcat caggttgcca atgcctatgg caaaacggca accttcatgc cgaagccggt ctttggcgac aatggctccg gcatgcatgt tcacttctcg atctggaaag acggcaagcc gaccttcgca ggcaatgaat atgctggtct gtcggaaaac tgcctctact tcattggcgg cgtcatcaag cacgcaaaag ctgtgaatgc cttcaccaac ccgtccacca actcctacaa gcgtctggtg ccgggctatg aagcgcctgt tctgctggcc tattccgcac gcaaccgttc ggcttcctgc cgcattccat tcggctcctc gccgaaatcg aagcgtctcg aagtccgctt cccggatccg tccgccaacc cttatctctg cttctcggcc cttctgatgg cgggccttga cggcatcaag aacaagatcc atccgggcca ggccatggac aaggatctct acgacctgcc gccgaaggaa ctgaagcaga ttccgaccgt ctgcggctcc ctgcgtgaag ctctacagtc gctcaacaag gatcgtgaat tcctcaaggc tggcggcgtg ttctccgacg accagatcga cagcttcatc gagctgaaaa tggctgaagt gatgcgtttt gaaatgacgc cgcacccggt cgagttcgat atgtattact cggtaaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BrabA.00111.a.A4.GE28062
HARVESTED ON: 12/7/2009
SEQUENCED ON: 12/23/2009
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 61
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTTANDILK QIKDNDVKFV DLRFTDPKGK LHHVTMDIGL VDEDMFVDGV MFDGSSIAGW KAINESDMVL MPDPETAHID PFFAQSTLVI LCDILEPLSG ESYSRDPRTT AKKAEAYLKS LGIGDTVYVG PEAEFFVFDD VKFKADPFNT GFKLDSAELP SNDDTDYETG NLGHRPRVKG GYFPVPPIDS LQDMRSEMLT VLSEMGVTVE KHHHEVASAQ HELGTKFDTL VRNADKMQIQ KYVVHXVANA YGKTATFMPK PVFGDNGSGM HVHFSIWK
Validated NT Sequence
tttaagaagg aganntacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgacgac tgccaacgat attctgaaac agatcaaaga taacgacgtc aagttcgtcg accttcgctt caccgatccg aagggcaagc tccatcacgt tacgatggat atcggcctcg tggacgaaga catgttcgtc gacggtgtca tgttcgacgg ctcttcgatc gcaggctgga aggccatcaa cgaatccgac atggttttga tgcccgatcc ggaaacggct catatcgacc cgttcttcgc ccagtccacg cttgtcattc tctgcgatat ccttgagccg ctttccggcg aaagctacag ccgtgatccg cgtacgactg ccaagaaggc cgaagcctat ctgaaatcgc tcggcattgg cgatacggtc tatgtcggcc cggaagccga attcttcgtc ttcgacgatg tgaagttcaa ggccgatccg ttcaatacgg gcttcaagct cgatagcgcc gaattgccat ccaacgacga caccgattat gaaaccggca atctcggcca tcgtccgcgc gtcaagggcg gctatttccc cgttccgcca atcgacagct tgcaggacat gcgttccgaa atgctgaccg tgctctcgga aatgggtgtg acggttgaaa agcaccacca tgaagtcgct tccgcgcagc acgaactggg cacgaaattc gacacgctgg tgcgcaacgc cgacaagatg cagattcaga aatatgtcgt gcatcangtt gccaatgcct atggcaaaac ggcaaccttc atgccgaagc cggtctttgg cgacaatggc tccggnatgc atgttcactt ctcgatctgg aaaga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTTANDILK QIKDNDVKFV DLRFTDPKGK LHHVTMDIGL VDEDMFVDGV MFDGSSIAGW KAINESDMVL MPDPETAHID PFFAQSTLVI LCDILEPLSG ESYSRDPRTT AKKAEAYLKS LGIGDTVYVG PEAEFFVFDD VKFKADPFNT GFKLDSAELP SNDDTDYETG NLGHRPRVKG GYFPVPPIDS LQDMRSEMLT VLSEMGVTVE KHHHEVASAQ HELGTKFDTL VRNADKMQIQ KYVVHQVANA YGKTATFMPK PVFGDNGSGM HVHFSIWKDG KPTFAGNEYA GLSENCLYFI GGVIKHAKAV NAFTNPSTNS YKRLVPGYEA PVLLAYSARN RSASCRIPFG SSPKSKRLEV RFPDPSANPY LCFSALLMAG LDGIKNKIHP GQAMDKDLYD LPPKELKQIP TVCGSLREAL QSLNKDREFL KAGGVFSDDQ IDSFIELKMA EVMRFEMTPH PVEFDMYY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgactgcc aacgatattc tgaaacagat caaagataac gacgtcaagt tcgtcgacct tcgcttcacc gatccgaagg gcaagctcca tcacgttacg atggatatcg gcctcgtgga cgaagacatg ttcgtcgacg gtgtcatgtt cgacggctct tcgatcgcag gctggaaggc catcaacgaa tccgacatgg ttttgatgcc cgatccggaa acggctcata tcgacccgtt cttcgcccag tccacgcttg tcattctctg cgatatcctt gagccgcttt ccggcgaaag ctacagccgt gatccgcgta cgactgccaa gaaggccgaa gcctatctga aatcgctcgg cattggcgat acggtctatg tcggcccgga agccgaattc ttcgtcttcg acgatgtgaa gttcaaggcc gatccgttca atacgggctt caagctcgat agcgccgaat tgccatccaa cgacgacacc gattatgaaa ccggcaatct cggccatcgt ccgcgcgtca agggcggcta tttccccgtt ccgccaatcg acagcttgca ggacatgcgt tccgaaatgc tgaccgtgct ctcggaaatg ggtgtgacgg ttgaaaagca ccaccatgaa gtcgcttccg cgcagcacga actgggcacg aaattcgaca cgctggtgcg caacgccgac aagatgcaga ttcagaaata tgtcgtgcat caggttgcca atgcctatgg caaaacggca accttcatgc cgaagccggt ctttggcgac aatggctccg gcatgcatgt tcacttctcg atctggaaag acggcaagcc gaccttcgca ggcaatgaat atgctggtct gtcggaaaac tgcctctact tcattggcgg cgtcatcaag cacgcaaaag ctgtgaatgc cttcaccaac ccgtccacca actcctacaa gcgtctggtg ccgggctatg aagcgcctgt tctgctggcc tattccgcac gcaaccgttc ggcttcctgc cgcattccat tcggctcctc gccgaaatcg aagcgtctcg aagtccgctt cccggatccg tccgccaacc cttatctctg cttctcggcc cttctgatgg cgggccttga cggcatcaag aacaagatcc atccgggcca ggccatggac aaggatctct acgacctgcc gccgaaggaa ctgaagcaga ttccgaccgt ctgcggctcc ctgcgtgaag ctctacagtc gctcaacaag gatcgtgaat tcctcaaggc tggcggcgtg ttctccgacg accagatcga cagcttcatc gagctgaaaa tggctgaagt gatgcgtttt gaaatgacgc cgcacccggt cgagttcgat atgtattaca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for BrabA.00111.a.A5.GE28063
HARVESTED ON: 12/7/2009
SEQUENCED ON: 12/23/2009
EXPECTED MW: 52kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDVKFVDLR FTDPKGKLHH VTMDIGLVDE DMFVDGVMFD GSSIAGWKAI NESDMVLMPD PETAHIDPFF AQSTLVILCD ILEPLSGESY SRDPRTTAKK AEAYLKSLGI GDTVYVGPEA EFFVFDDVKF KADPFNTGFK LDSAELPSND DTDYETGNLG HRPRVKGGYF PVPPIDSLQD MRSEMLTVLS EMGVTVEKHH HEVASAQHEL GTKFDTLVRN ADKMQIQKYV VHQVANAYGK TATFMPKPVF GDNGSGMHVH FSIWKDGKPT FAGNEYAGLS ENCLYFIGGV IKHAKAVNAF TNPSTNSYKR LVPGYEAPVL LAYSARNRSA SCRIPFGSSP KSKRLEVRFP DPSANPYLCF SALLMAGLDG IKNKIHPGQA MDKDLYDLPP KELKQIPTVC GSLREALQSL NKDREFLKAG GVFSDDQIDS FIELKMAEVM RFEMTPHPVE FDMYYSV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacgtcaag ttcgtcgacc ttcgcttcac cgatccgaag ggcaagctcc atcacgttac gatggatatc ggcctcgtgg acgaagacat gttcgtcgac ggtgtcatgt tcgacggctc ttcgatcgca ggctggaagg ccatcaacga atccgacatg gttttgatgc ccgatccgga aacggctcat atcgacccgt tcttcgccca gtccacgctt gtcattctct gcgatatcct tgagccgctt tccggcgaaa gctacagccg tgatccgcgt acgactgcca agaaggccga agcctatctg aaatcgctcg gcattggcga tacggtctat gtcggcccgg aagccgaatt cttcgtcttc gacgatgtga agttcaaggc cgatccgttc aatacgggct tcaagctcga tagcgccgaa ttgccatcca acgacgacac cgattatgaa accggcaatc tcggccatcg tccgcgcgtc aagggcggct atttccccgt tccgccaatc gacagcttgc aggacatgcg ttccgaaatg ctgaccgtgc tctcggaaat gggtgtgacg gttgaaaagc accaccatga agtcgcttcc gcgcagcacg aactgggcac gaaattcgac acgctggtgc gcaacgccga caagatgcag attcagaaat atgtcgtgca tcaggttgcc aatgcctatg gcaaaacggc aaccttcatg ccgaagccgg tctttggcga caatggctcc ggcatgcatg ttcacttctc gatctggaaa gacggcaagc cgaccttcgc aggcaatgaa tatgctggtc tgtcggaaaa ctgcctctac ttcattggcg gcgtcatcaa gcacgcaaaa gctgtgaatg ccttcaccaa cccgtccacc aactcctaca agcgtctggt gccgggctat gaagcgcctg ttctgctggc ctattccgca cgcaaccgtt cggcttcctg ccgcattcca ttcggctcct cgccgaaatc gaagcgtctc gaagtccgct tcccggatcc gtccgccaac ccttatctct gcttctcggc ccttctgatg gcgggccttg acggcatcaa gaacaagatc catccgggcc aggccatgga caaggatctc tacgacctgc cgccgaagga actgaagcag attccgaccg tctgcggctc cctgcgtgaa gctctacagt cgctcaacaa ggatcgtgaa ttcctcaagg ctggcggcgt gttctccgac gaccagatcg acagcttcat cgagctgaaa atggctgaag tgatgcgttt tgaaatgacg ccgcacccgg tcgagttcga tatgtattac tcggtaaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BrabA.00111.a.A6.GE28064
HARVESTED ON: 12/7/2009
SEQUENCED ON: 2/24/2010
EXPECTED MW: 52kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 68
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDVKFVDLR FTDPKGKLHH VTMDIGLVDE DMFVDGVMFD GSSIAGWKAI NESDMVLMPD PETAHIDPFF AQSTLVILCD ILEPLSGESY SRDPRTTAKK AEAYLKSLGI GDTVYVGPEA EFFVFDDVKF KADPFNTGFK LDSAELPSND DTDYETGNLG HRPRVKGGYF PVPPIDSLQD MRSEMLTVLS EMGVTVEKHH HEVASAQHEL GTKFDTLVRN ADKMQIQKYV VHQVANAYGK TATFMPKPVF GDNGSGMHVH FSIWKDGKPT FAGNEYAGLS ENCLYFIGGV IKHAKAVNAF XXP
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggacg tcaagttcgt cgaccttcgc ttcaccgatc cgaagggcaa gctccatcac gttacgatgg atatcggcct cgtggacgaa gacatgttcg tcgacggtgt catgttcgac ggctcttcga tcgcaggctg gaaggccatc aacgaatccg acatggtttt gatgcccgat ccggaaacgg ctcatatcga cccgttcttc gcccagtcca cgcttgtcat tctctgcgat atccttgagc cgctttccgg cgaaagctac agccgtgatc cgcgtacgac tgccaagaag gccgaagcct atctgaaatc gctcggcatt ggcgatacgg tctatgtcgg cccggaagcc gaattcttcg tcttcgacga tgtgaagttc aaggccgatc cgttcaatac gggcttcaag ctcgatagcg ccgaattgcc atccaacgac gacaccgatt atgaaaccgg caatctcggc catcgtccgc gcgtcaaggg cggctatttc cccgttccgc caatcgacag cttgcaggac atgcgttccg aaatgctgac cgtgctctcg gaaatgggtg tgacggttga aaagcaccac catgaagtcg cttccgcgca gcacgaactg ggcacgaaat tcgacacgct ggtgcgcaac gccgacaaga tgcagattca gaaatatgtc gtgcatcagg ttgccaatgc ctatggcaaa acggcaacct tcatgccgaa gccggtcttt ggcgacaatg gctccggcat gcatgttcac ttctcgatct ggaaagacgg caagccgacc ttcgcaggca atgaatatgc tggtctgtcg gaaaactgcc tctacttcat tggcggcgtc atcaagcacg caaaagctgt gaatgccttc nncnacccgt cc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDVKFVDLR FTDPKGKLHH VTMDIGLVDE DMFVDGVMFD GSSIAGWKAI NESDMVLMPD PETAHIDPFF AQSTLVILCD ILEPLSGESY SRDPRTTAKK AEAYLKSLGI GDTVYVGPEA EFFVFDDVKF KADPFNTGFK LDSAELPSND DTDYETGNLG HRPRVKGGYF PVPPIDSLQD MRSEMLTVLS EMGVTVEKHH HEVASAQHEL GTKFDTLVRN ADKMQIQKYV VHQVANAYGK TATFMPKPVF GDNGSGMHVH FSIWKDGKPT FAGNEYAGLS ENCLYFIGGV IKHAKAVNAF TNPSTNSYKR LVPGYEAPVL LAYSARNRSA SCRIPFGSSP KSKRLEVRFP DPSANPYLCF SALLMAGLDG IKNKIHPGQA MDKDLYDLPP KELKQIPTVC GSLREALQSL NKDREFLKAG GVFSDDQIDS FIELKMAEVM RFEMTPHPVE FDMYY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacgtcaag ttcgtcgacc ttcgcttcac cgatccgaag ggcaagctcc atcacgttac gatggatatc ggcctcgtgg acgaagacat gttcgtcgac ggtgtcatgt tcgacggctc ttcgatcgca ggctggaagg ccatcaacga atccgacatg gttttgatgc ccgatccgga aacggctcat atcgacccgt tcttcgccca gtccacgctt gtcattctct gcgatatcct tgagccgctt tccggcgaaa gctacagccg tgatccgcgt acgactgcca agaaggccga agcctatctg aaatcgctcg gcattggcga tacggtctat gtcggcccgg aagccgaatt cttcgtcttc gacgatgtga agttcaaggc cgatccgttc aatacgggct tcaagctcga tagcgccgaa ttgccatcca acgacgacac cgattatgaa accggcaatc tcggccatcg tccgcgcgtc aagggcggct atttccccgt tccgccaatc gacagcttgc aggacatgcg ttccgaaatg ctgaccgtgc tctcggaaat gggtgtgacg gttgaaaagc accaccatga agtcgcttcc gcgcagcacg aactgggcac gaaattcgac acgctggtgc gcaacgccga caagatgcag attcagaaat atgtcgtgca tcaggttgcc aatgcctatg gcaaaacggc aaccttcatg ccgaagccgg tctttggcga caatggctcc ggcatgcatg ttcacttctc gatctggaaa gacggcaagc cgaccttcgc aggcaatgaa tatgctggtc tgtcggaaaa ctgcctctac ttcattggcg gcgtcatcaa gcacgcaaaa gctgtgaatg ccttcaccaa cccgtccacc aactcctaca agcgtctggt gccgggctat gaagcgcctg ttctgctggc ctattccgca cgcaaccgtt cggcttcctg ccgcattcca ttcggctcct cgccgaaatc gaagcgtctc gaagtccgct tcccggatcc gtccgccaac ccttatctct gcttctcggc ccttctgatg gcgggccttg acggcatcaa gaacaagatc catccgggcc aggccatgga caaggatctc tacgacctgc cgccgaagga actgaagcag attccgaccg tctgcggctc cctgcgtgaa gctctacagt cgctcaacaa ggatcgtgaa ttcctcaagg ctggcggcgt gttctccgac gaccagatcg acagcttcat cgagctgaaa atggctgaag tgatgcgttt tgaaatgacg ccgcacccgg tcgagttcga tatgtattac aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for BrabA.00111.a.B4.GE43038
HARVESTED ON: 4/19/2019
SEQUENCED ON: 4/23/2019
EXPECTED MW: 53kDa
OBSERVED MW: 53kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHTT ANDILKQIKD NDVKFVDLRF TDPKGKLHHV TMDIGLVDED MFVDGVMFDG SSIAGWKAIN ESDMVLMPDP ETAHIDPFFA QSTLVILCDI LEPLSGESYS RDPRTTAKKA EAYLKSLGIG DTVYVGPEAE FFVFDDVKFK ADPFNTGFKL DSAELPSNDD TDYETGNLGH RPRVKGGYFP VPPIDSLQDM RSEMLTVLSE MGVTVEKHHH EVASAQHELG TKFDTLVRNA DKMQIQKYVV HQVANAYGKT ATFMPKPVFG DNGSGMHVHF SIWKDGKPTF AGNEYAGLSE NCLYFIGGVI KHAKAVNAFT NPSTNSYKRL VPGYEAPVLL AYSARNRSAS CRIPFGSSPK SKRLEVRFPD PSANPYLCFS ALLMAGLDGI KNKIHPGQAM DKDLYDLPPK ELKQIPTVCG SLREALQSLN KDREFLKAGG VFSDDQIDSF IELKMAEVMR FEMTPHPVEF DMYY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catacgactg ccaacgatat tctgaaacag atcaaagata acgacgtcaa gttcgtcgac cttcgcttca ccgatccgaa gggcaagctc catcacgtta cgatggatat cggcctcgtg gacgaagaca tgttcgtcga cggtgtcatg ttcgacggct cttcgatcgc aggctggaag gccatcaacg aatccgacat ggttttgatg cccgatccgg aaacggctca tatcgacccg ttcttcgccc agtccacgct tgtcattctc tgcgatatcc ttgagccgct ttccggcgaa agctacagcc gtgatccgcg tacgactgcc aagaaggccg aagcctatct gaaatcgctc ggcattggcg atacggtcta tgtcggcccg gaagccgaat tcttcgtctt cgacgatgtg aagttcaagg ccgatccgtt caatacgggc ttcaagctcg atagcgccga attgccatcc aacgacgaca ccgattatga aaccggcaat ctcggccatc gtccgcgcgt caagggcggc tatttccccg ttccgccaat cgacagcttg caggacatgc gttccgaaat gctgaccgtg ctctcggaaa tgggtgtgac ggttgaaaag caccaccatg aagtcgcttc cgcgcagcac gaactgggca cgaaattcga cacgctggtg cgcaacgccg acaagatgca gattcagaaa tatgtcgtgc atcaggttgc caatgcctat ggcaaaacgg caaccttcat gccgaagccg gtctttggcg acaatggctc cggcatgcat gttcacttct cgatctggaa agacggcaag ccgaccttcg caggcaatga atatgctggt ctgtcggaaa actgcctcta cttcattggc ggcgtcatca agcacgcaaa agctgtgaat gccttcacca acccgtccac caactcctac aagcgtctgg tgccgggcta tgaagcgcct gttctgctgg cctattccgc acgcaaccgt tcggcttcct gccgcattcc attcggctcc tcgccgaaat cgaagcgtct cgaagtccgc ttcccggatc cgtccgccaa cccttatctc tgcttctcgg cccttctgat ggcgggcctt gacggcatca agaacaagat ccatccgggc caggccatgg acaaggatct ctacgacctg ccgccgaagg aactgaagca gattccgacc gtctgcggct ccctgcgtga agctctacag tcgctcaaca aggatcgtga attcctcaag gctggcggcg tgttctccga cgaccagatc gacagcttca tcgagctgaa aatggctgaa gtgatgcgtt ttgaaatgac gccgcacccg gtcgagttcg atatgtatta ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for BrabA.00111.a.A1.PS00110
PURIFICATION DATe: 10/10/2008
CONCENTRATION: 8.73mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTTANDILK QIKDNDVKFV DLRFTDPKGK LHHVTMDIGL VDEDMFVDGV MFDGSSIAGW KAINESDMVL MPDPETAHID PFFAQSTLVI LCDILEPLSG ESYSRDPRTT AKKAEAYLKS LGIGDTVYVG PEAEFFVFDD VKFKADPFNT GFKLDSAELP SNDDTDYETG NLGHRPRVKG GYFPVPPIDS LQDMRSEMLT VLSEMGVTVE KHHHEVASAQ HELGTKFDTL VRNADKMQIQ KYVVHQVANA YGKTATFMPK PVFGDNGSGM HVHFSIWKDG KPTFAGNEYA GLSENCLYFI GGVIKHAKAV NAFTNPSTNS YKRLVPGYEA PVLLAYSARN RSASCRIPFG SSPKSKRLEV RFPDPSANPY LCFSALLMAG LDGIKNKIHP GQAMDKDLYD LPPKELKQIP TVCGSLREAL QSLNKDREFL KAGGVFSDDQ IDSFIELKMA EVMRFEMTPH PVEFDMYYSV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACGACTGCC AACGATATTC TGAAACAGAT CAAAGATAAC GACGTCAAGT TCGTCGACCT TCGCTTCACC GATCCGAAGG GCAAGCTCCA TCACGTTACG ATGGATATCG GCCTCGTGGA CGAAGACATG TTCGTCGACG GTGTCATGTT CGACGGCTCT TCGATCGCAG GCTGGAAGGC CATCAACGAA TCCGACATGG TTTTGATGCC CGATCCGGAA ACGGCTCATA TCGACCCGTT CTTCGCCCAG TCCACGCTTG TCATTCTCTG CGATATCCTT GAGCCGCTTT CCGGCGAAAG CTACAGCCGT GATCCGCGTA CGACTGCCAA GAAGGCCGAA GCCTATCTGA AATCGCTCGG CATTGGCGAT ACGGTCTATG TCGGCCCGGA AGCCGAATTC TTCGTCTTCG ACGATGTGAA GTTCAAGGCC GATCCGTTCA ATACGGGCTT CAAGCTCGAT AGCGCCGAAT TGCCATCCAA CGACGACACC GATTATGAAA CCGGCAATCT CGGCCATCGT CCGCGCGTCA AGGGCGGCTA TTTCCCCGTT CCGCCAATCG ACAGCTTGCA GGACATGCGT TCCGAAATGC TGACCGTGCT CTCGGAAATG GGTGTGACGG TTGAAAAGCA CCACCATGAA GTCGCTTCCG CGCAGCACGA ACTGGGCACG AAATTCGACA CGCTGGTGCG CAACGCCGAC AAGATGCAGA TTCAGAAATA TGTCGTGCAT CAGGTTGCCA ATGCCTATGG CAAAACGGCA ACCTTCATGC CGAAGCCGGT CTTTGGCGAC AATGGCTCCG GCATGCATGT TCACTTCTCG ATCTGGAAAG ACGGCAAGCC GACCTTCGCA GGCAATGAAT ATGCTGGTCT GTCGGAAAAC TGCCTCTACT TCATTGGCGG CGTCATCAAG CACGCAAAAG CTGTGAATGC CTTCACCAAC CCGTCCACCA ACTCCTACAA GCGTCTGGTG CCGGGCTATG AAGCGCCTGT TCTGCTGGCC TATTCCGCAC GCAACCGTTC GGCTTCCTGC CGCATTCCAT TCGGCTCCTC GCCGAAATCG AAGCGTCTCG AAGTCCGCTT CCCGGATCCG TCCGCCAACC CTTATCTCTG CTTCTCGGCC CTTCTGATGG CGGGCCTTGA CGGCATCAAG AACAAGATCC ATCCGGGCCA GGCCATGGAC AAGGATCTCT ACGACCTGCC GCCGAAGGAA CTGAAGCAGA TTCCGACCGT CTGCGGCTCC CTGCGTGAAG CTCTACAGTC GCTCAACAAG GATCGTGAAT TCCTCAAGGC TGGCGGCGTG TTCTCCGACG ACCAGATCGA CAGCTTCATC GAGCTGAAAA TGGCTGAAGT GATGCGTTTT GAAATGACGC CGCACCCGGT CGAGTTCGAT ATGTATTACT CGGTATAAAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA
Details for BrabA.00111.a.A1.PW25440
PURIFICATION DATe: 9/2/2008
CONCENTRATION: 9mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 50µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTTANDILK QIKDNDVKFV DLRFTDPKGK LHHVTMDIGL VDEDMFVDGV MFDGSSIAGW KAINESDMVL MPDPETAHID PFFAQSTLVI LCDILEPLSG ESYSRDPRTT AKKAEAYLKS LGIGDTVYVG PEAEFFVFDD VKFKADPFNT GFKLDSAELP SNDDTDYETG NLGHRPRVKG GYFPVPPIDS LQDMRSEMLT VLSEMGVTVE KHHHEVASAQ HELGTKFDTL VRNADKMQIQ KYVVHQVANA YGKTATFMPK PVFGDNGSGM HVHFSIWKDG KPTFAGNEYA GLSENCLYFI GGVIKHAKAV NAFTNPSTNS YKRLVPGYEA PVLLAYSARN RSASCRIPFG SSPKSKRLEV RFPDPSANPY LCFSALLMAG LDGIKNKIHP GQAMDKDLYD LPPKELKQIP TVCGSLREAL QSLNKDREFL KAGGVFSDDQ IDSFIELKMA EVMRFEMTPH PVEFDMYYSV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACGACTGCC AACGATATTC TGAAACAGAT CAAAGATAAC GACGTCAAGT TCGTCGACCT TCGCTTCACC GATCCGAAGG GCAAGCTCCA TCACGTTACG ATGGATATCG GCCTCGTGGA CGAAGACATG TTCGTCGACG GTGTCATGTT CGACGGCTCT TCGATCGCAG GCTGGAAGGC CATCAACGAA TCCGACATGG TTTTGATGCC CGATCCGGAA ACGGCTCATA TCGACCCGTT CTTCGCCCAG TCCACGCTTG TCATTCTCTG CGATATCCTT GAGCCGCTTT CCGGCGAAAG CTACAGCCGT GATCCGCGTA CGACTGCCAA GAAGGCCGAA GCCTATCTGA AATCGCTCGG CATTGGCGAT ACGGTCTATG TCGGCCCGGA AGCCGAATTC TTCGTCTTCG ACGATGTGAA GTTCAAGGCC GATCCGTTCA ATACGGGCTT CAAGCTCGAT AGCGCCGAAT TGCCATCCAA CGACGACACC GATTATGAAA CCGGCAATCT CGGCCATCGT CCGCGCGTCA AGGGCGGCTA TTTCCCCGTT CCGCCAATCG ACAGCTTGCA GGACATGCGT TCCGAAATGC TGACCGTGCT CTCGGAAATG GGTGTGACGG TTGAAAAGCA CCACCATGAA GTCGCTTCCG CGCAGCACGA ACTGGGCACG AAATTCGACA CGCTGGTGCG CAACGCCGAC AAGATGCAGA TTCAGAAATA TGTCGTGCAT CAGGTTGCCA ATGCCTATGG CAAAACGGCA ACCTTCATGC CGAAGCCGGT CTTTGGCGAC AATGGCTCCG GCATGCATGT TCACTTCTCG ATCTGGAAAG ACGGCAAGCC GACCTTCGCA GGCAATGAAT ATGCTGGTCT GTCGGAAAAC TGCCTCTACT TCATTGGCGG CGTCATCAAG CACGCAAAAG CTGTGAATGC CTTCACCAAC CCGTCCACCA ACTCCTACAA GCGTCTGGTG CCGGGCTATG AAGCGCCTGT TCTGCTGGCC TATTCCGCAC GCAACCGTTC GGCTTCCTGC CGCATTCCAT TCGGCTCCTC GCCGAAATCG AAGCGTCTCG AAGTCCGCTT CCCGGATCCG TCCGCCAACC CTTATCTCTG CTTCTCGGCC CTTCTGATGG CGGGCCTTGA CGGCATCAAG AACAAGATCC ATCCGGGCCA GGCCATGGAC AAGGATCTCT ACGACCTGCC GCCGAAGGAA CTGAAGCAGA TTCCGACCGT CTGCGGCTCC CTGCGTGAAG CTCTACAGTC GCTCAACAAG GATCGTGAAT TCCTCAAGGC TGGCGGCGTG TTCTCCGACG ACCAGATCGA CAGCTTCATC GAGCTGAAAA TGGCTGAAGT GATGCGTTTT GAAATGACGC CGCACCCGGT CGAGTTCGAT ATGTATTACT CGGTATAAAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA
Details for BrabA.00111.a.A3.PW28709
PURIFICATION DATe: 2/1/2010
CONCENTRATION: 3.5mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: data unavailable
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTTANDILK QIKDNDVKFV DLRFTDPKGK LHHVTMDIGL VDEDMFVDGV MFDGSSIAGW KAINESDMVL MPDPETAHID PFFAQSTLVI LCDILEPLSG ESYSRDPRTT AKKAEAYLKS LGIGDTVYVG PEAEFFVFDD VKFKADPFNT GFKLDSAELP SNDDTDYETG NLGHRPRVKG GYFPVPPIDS LQDMRSEMLT VLSEMGVTVE KHHHEVASAQ HELGTKFDTL VRNADKMQIQ KYVVHQVANA YGKTATFMPK PVFGDNGSGM HVHFSIWKDG KPTFAGNEYA GLSENCLYFI GGVIKHAKAV NAFTNPSTNS YKRLVPGYEA PVLLAYSARN RSASCRIPFG SSPKSKRLEV RFPDPSANPY LCFSALLMAG LDGIKNKIHP GQAMDKDLYD LPPKELKQIP TVCGSLREAL QSLNKDREFL KAGGVFSDDQ IDSFIELKMA EVMRFEMTPH PVEFDMYYSV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACGACTGCC AACGATATTC TGAAACAGAT CAAAGATAAC GACGTCAAGT TCGTCGACCT TCGCTTCACC GATCCGAAGG GCAAGCTCCA TCACGTTACG ATGGATATCG GCCTCGTGGA CGAAGACATG TTCGTCGACG GTGTCATGTT CGACGGCTCT TCGATCGCAG GCTGGAAGGC CATCAACGAA TCCGACATGG TTTTGATGCC CGATCCGGAA ACGGCTCATA TCGACCCGTT CTTCGCCCAG TCCACGCTTG TCATTCTCTG CGATATCCTT GAGCCGCTTT CCGGCGAAAG CTACAGCCGT GATCCGCGTA CGACTGCCAA GAAGGCCGAA GCCTATCTGA AATCGCTCGG CATTGGCGAT ACGGTCTATG TCGGCCCGGA AGCCGAATTC TTCGTCTTCG ACGATGTGAA GTTCAAGGCC GATCCGTTCA ATACGGGCTT CAAGCTCGAT AGCGCCGAAT TGCCATCCAA CGACGACACC GATTATGAAA CCGGCAATCT CGGCCATCGT CCGCGCGTCA AGGGCGGCTA TTTCCCCGTT CCGCCAATCG ACAGCTTGCA GGACATGCGT TCCGAAATGC TGACCGTGCT CTCGGAAATG GGTGTGACGG TTGAAAAGCA CCACCATGAA GTCGCTTCCG CGCAGCACGA ACTGGGCACG AAATTCGACA CGCTGGTGCG CAACGCCGAC AAGATGCAGA TTCAGAAATA TGTCGTGCAT CAGGTTGCCA ATGCCTATGG CAAAACGGCA ACCTTCATGC CGAAGCCGGT CTTTGGCGAC AATGGCTCCG GCATGCATGT TCACTTCTCG ATCTGGAAAG ACGGCAAGCC GACCTTCGCA GGCAATGAAT ATGCTGGTCT GTCGGAAAAC TGCCTCTACT TCATTGGCGG CGTCATCAAG CACGCAAAAG CTGTGAATGC CTTCACCAAC CCGTCCACCA ACTCCTACAA GCGTCTGGTG CCGGGCTATG AAGCGCCTGT TCTGCTGGCC TATTCCGCAC GCAACCGTTC GGCTTCCTGC CGCATTCCAT TCGGCTCCTC GCCGAAATCG AAGCGTCTCG AAGTCCGCTT CCCGGATCCG TCCGCCAACC CTTATCTCTG CTTCTCGGCC CTTCTGATGG CGGGCCTTGA CGGCATCAAG AACAAGATCC ATCCGGGCCA GGCCATGGAC AAGGATCTCT ACGACCTGCC GCCGAAGGAA CTGAAGCAGA TTCCGACCGT CTGCGGCTCC CTGCGTGAAG CTCTACAGTC GCTCAACAAG GATCGTGAAT TCCTCAAGGC TGGCGGCGTG TTCTCCGACG ACCAGATCGA CAGCTTCATC GAGCTGAAAA TGGCTGAAGT GATGCGTTTT GAAATGACGC CGCACCCGGT CGAGTTCGAT ATGTATTACT CGGTAAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA