BrabA.00102.a
DAHP synthetase I/KDSA superfamily:2-dehydro-3-deoxyphosphooctonate aldolase
| CENTER ID: | BrabA.00102.a |
| ORGANISM: | Brucella melitensis biovar Abortus 2308 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.00102.a.A1.GE25400 | Full length( BrabA.00102.a ) | 1 | 277 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.00102.a.A1.PS00063 | Full length( BrabA.00102.a ) | 1 | 277 |
Structures
3FS2
DEPOSITED: 1/9/2009
DETERMINATION: XRay
CLONE: BrabA.00102.a.A1.GE25400
PROTEIN: BrabA.00102.a.A1.PS00063
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_130461 |
| PATRIC ID: | fig|359391.4.peg.1204 |
| RefSeq: | YP_414547 |
| UniProt: | Q2YPU9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVTANSTVKV GNVTFSNSAP LALIAGPCQM ETRDHAFEMA GRLKEMTDKL GIGLVYKSSF DKANRTSLKA ARGIGLEKAL EVFSDLKKEY GFPVLTDIHT EEQCAAVAPV VDVLQIPAFL CRQTDLLIAA ARTGRVVNVK KGQFLAPWDM KNVLAKITES GNPNVLATER GVSFGYNTLV SDMRALPIMA GLGAPVIFDA THSVQQPGGQ GGSTGGQREF VETLARAAVA VGVAGFFIET HEDPDNAPSD GPNMVPIDKM PALLEKLMAF DRIAKAL
NT Sequence
ATGGTGACTG CCAATTCCAC GGTTAAAGTC GGTAACGTCA CGTTTTCCAA CAGCGCGCCT TTGGCGCTGA TCGCTGGCCC TTGCCAGATG GAAACGCGCG ACCATGCCTT TGAAATGGCG GGTCGCCTGA AGGAAATGAC CGATAAGCTG GGTATTGGCC TTGTTTACAA GTCCAGCTTC GATAAGGCCA ACCGCACTTC GCTGAAGGCC GCGCGCGGCA TCGGTCTTGA AAAGGCGCTG GAAGTGTTCA GCGATCTCAA GAAGGAATAT GGTTTTCCCG TTCTGACCGA TATCCATACC GAAGAGCAGT GTGCGGCTGT CGCTCCGGTT GTCGATGTGC TTCAGATCCC GGCATTTCTT TGCCGCCAGA CCGATCTCCT GATTGCCGCC GCCAGAACTG GCCGTGTGGT GAATGTGAAG AAAGGCCAGT TTCTTGCGCC TTGGGACATG AAGAATGTTC TTGCCAAGAT CACCGAGAGC GGCAATCCGA ATGTTCTGGC CACGGAGCGC GGCGTGTCGT TCGGCTATAA TACGCTTGTT TCGGATATGC GCGCATTGCC AATCATGGCT GGCCTTGGTG CGCCGGTGAT TTTTGATGCG ACTCACTCCG TCCAGCAGCC GGGCGGGCAG GGCGGTTCTA CGGGTGGGCA GCGTGAATTC GTCGAAACGC TGGCGCGTGC CGCAGTTGCA GTGGGCGTGG CGGGCTTCTT CATCGAAACC CACGAAGATC CCGATAATGC TCCGTCCGAC GGCCCCAACA TGGTGCCGAT CGACAAGATG CCGGCGCTTC TGGAAAAGCT GATGGCATTC GATCGCATTG CCAAGGCGCT G
Details for BrabA.00102.a.A1.GE25400
| HARVESTED ON: | 6/30/2008 |
| SEQUENCED ON: | 6/16/2009 |
| EXPECTED MW: | 31kDa |
| OBSERVED MW: | 32kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 95 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTANSTVK VGNVTFSNSA PLALIAGPCQ METRDHAFEM AGRLKEMTDK LGIGLVYKSS FDKANRTSLK AARGIGLEKA LEVFSDLKKE YGFPVLTDIH TEEQCAAVAP VVDVLQIPAF LCRQTDLLIA AARTGRVVNV KKGQFLAPWD MKNVLAKITE SGNPNVLATE RGVSFGYNTL VSDMRALPIM AGLGAPVIFD ATHSVQQPGG QGGSTGGQRE FVETLARAAX AVGVAGFFIE THEDXXNAPS DGPNMVPXDK XPALLE
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga ctgccaattc cacggttaaa gtcggtaacg tcacgttttc caacagcgcg cctttggcgc tgatcgctgg cccttgccag atggaaacgc gcgaccatgc ctttgaaatg gcgggtcgcc tgaaggaaat gaccgataag ctgggtattg gccttgttta caagtccagc ttcgataagg ccaaccgcac ttcgctgaag gccgcgcgcg gcatcggtct tgaaaaggcg ctggaagtgt tcagcgatct caagaaggaa tatggttttc ccgttctgac cgatatccat accgaagagc agtgtgcggc tgtcgctccg gttgtcgatg tgcttcagat cccggcattt ctttgccgcc agaccgatct cctgattgcc gccgccagaa ctggccgtgt ggtgaatgtg aagaaaggcc agtttcttgc gccttgggac atgaagaatg ttcttgccaa gatcaccgag agcggcaatc cgaatgttct ggccacggag cgcggcgtgt cgttcggcta taatacgctt gtttcggata tgcgcgcatt gccaatcatg gctggccttg gtgcgccggt gatttttgat gcgactcact ccgtccagca gccgggcggg cagggcggtt ctacgggtgg gcagcgtgaa ttcgtcgaaa cgctggcgcg tgccgcantt gcagtgggcg tggcgggctt cttcatcgaa acccacgaag atnccnataa tgctccgtcc gacggcccca acatggtgcc nntcgacaag angccggcnc ttctggaaaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTANSTVK VGNVTFSNSA PLALIAGPCQ METRDHAFEM AGRLKEMTDK LGIGLVYKSS FDKANRTSLK AARGIGLEKA LEVFSDLKKE YGFPVLTDIH TEEQCAAVAP VVDVLQIPAF LCRQTDLLIA AARTGRVVNV KKGQFLAPWD MKNVLAKITE SGNPNVLATE RGVSFGYNTL VSDMRALPIM AGLGAPVIFD ATHSVQQPGG QGGSTGGQRE FVETLARAAV AVGVAGFFIE THEDPDNAPS DGPNMVPIDK MPALLEKLMA FDRIAKAL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgactgcc aattccacgg ttaaagtcgg taacgtcacg ttttccaaca gcgcgccttt ggcgctgatc gctggccctt gccagatgga aacgcgcgac catgcctttg aaatggcggg tcgcctgaag gaaatgaccg ataagctggg tattggcctt gtttacaagt ccagcttcga taaggccaac cgcacttcgc tgaaggccgc gcgcggcatc ggtcttgaaa aggcgctgga agtgttcagc gatctcaaga aggaatatgg ttttcccgtt ctgaccgata tccataccga agagcagtgt gcggctgtcg ctccggttgt cgatgtgctt cagatcccgg catttctttg ccgccagacc gatctcctga ttgccgccgc cagaactggc cgtgtggtga atgtgaagaa aggccagttt cttgcgcctt gggacatgaa gaatgttctt gccaagatca ccgagagcgg caatccgaat gttctggcca cggagcgcgg cgtgtcgttc ggctataata cgcttgtttc ggatatgcgc gcattgccaa tcatggctgg ccttggtgcg ccggtgattt ttgatgcgac tcactccgtc cagcagccgg gcgggcaggg cggttctacg ggtgggcagc gtgaattcgt cgaaacgctg gcgcgtgccg cagttgcagt gggcgtggcg ggcttcttca tcgaaaccca cgaagatccc gataatgctc cgtccgacgg ccccaacatg gtgccgatcg acaagatgcc ggcgcttctg gaaaagctga tggcattcga tcgcattgcc aaggcgctgt aaaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for BrabA.00102.a.A1.PS00063
| PURIFICATION DATe: | 8/1/2008 |
| CONCENTRATION: | 28.8mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 15 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 95 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTANSTVK VGNVTFSNSA PLALIAGPCQ METRDHAFEM AGRLKEMTDK LGIGLVYKSS FDKANRTSLK AARGIGLEKA LEVFSDLKKE YGFPVLTDIH TEEQCAAVAP VVDVLQIPAF LCRQTDLLIA AARTGRVVNV KKGQFLAPWD MKNVLAKITE SGNPNVLATE RGVSFGYNTL VSDMRALPIM AGLGAPVIFD ATHSVQQPGG QGGSTGGQRE FVETLARAAX AVGVAGFFIE THEDXXNAPS DGPNMVPXDK XPALLE
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga ctgccaattc cacggttaaa gtcggtaacg tcacgttttc caacagcgcg cctttggcgc tgatcgctgg cccttgccag atggaaacgc gcgaccatgc ctttgaaatg gcgggtcgcc tgaaggaaat gaccgataag ctgggtattg gccttgttta caagtccagc ttcgataagg ccaaccgcac ttcgctgaag gccgcgcgcg gcatcggtct tgaaaaggcg ctggaagtgt tcagcgatct caagaaggaa tatggttttc ccgttctgac cgatatccat accgaagagc agtgtgcggc tgtcgctccg gttgtcgatg tgcttcagat cccggcattt ctttgccgcc agaccgatct cctgattgcc gccgccagaa ctggccgtgt ggtgaatgtg aagaaaggcc agtttcttgc gccttgggac atgaagaatg ttcttgccaa gatcaccgag agcggcaatc cgaatgttct ggccacggag cgcggcgtgt cgttcggcta taatacgctt gtttcggata tgcgcgcatt gccaatcatg gctggccttg gtgcgccggt gatttttgat gcgactcact ccgtccagca gccgggcggg cagggcggtt ctacgggtgg gcagcgtgaa ttcgtcgaaa cgctggcgcg tgccgcantt gcagtgggcg tggcgggctt cttcatcgaa acccacgaag atnccnataa tgctccgtcc gacggcccca acatggtgcc nntcgacaag angccggcnc ttctggaaaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTANSTVK VGNVTFSNSA PLALIAGPCQ METRDHAFEM AGRLKEMTDK LGIGLVYKSS FDKANRTSLK AARGIGLEKA LEVFSDLKKE YGFPVLTDIH TEEQCAAVAP VVDVLQIPAF LCRQTDLLIA AARTGRVVNV KKGQFLAPWD MKNVLAKITE SGNPNVLATE RGVSFGYNTL VSDMRALPIM AGLGAPVIFD ATHSVQQPGG QGGSTGGQRE FVETLARAAV AVGVAGFFIE THEDPDNAPS DGPNMVPIDK MPALLEKLMA FDRIAKAL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGACTGCC AATTCCACGG TTAAAGTCGG TAACGTCACG TTTTCCAACA GCGCGCCTTT GGCGCTGATC GCTGGCCCTT GCCAGATGGA AACGCGCGAC CATGCCTTTG AAATGGCGGG TCGCCTGAAG GAAATGACCG ATAAGCTGGG TATTGGCCTT GTTTACAAGT CCAGCTTCGA TAAGGCCAAC CGCACTTCGC TGAAGGCCGC GCGCGGCATC GGTCTTGAAA AGGCGCTGGA AGTGTTCAGC GATCTCAAGA AGGAATATGG TTTTCCCGTT CTGACCGATA TCCATACCGA AGAGCAGTGT GCGGCTGTCG CTCCGGTTGT CGATGTGCTT CAGATCCCGG CATTTCTTTG CCGCCAGACC GATCTCCTGA TTGCCGCCGC CAGAACTGGC CGTGTGGTGA ATGTGAAGAA AGGCCAGTTT CTTGCGCCTT GGGACATGAA GAATGTTCTT GCCAAGATCA CCGAGAGCGG CAATCCGAAT GTTCTGGCCA CGGAGCGCGG CGTGTCGTTC GGCTATAATA CGCTTGTTTC GGATATGCGC GCATTGCCAA TCATGGCTGG CCTTGGTGCG CCGGTGATTT TTGATGCGAC TCACTCCGTC CAGCAGCCGG GCGGGCAGGG CGGTTCTACG GGTGGGCAGC GTGAATTCGT CGAAACGCTG GCGCGTGCCG CAGTTGCAGT GGGCGTGGCG GGCTTCTTCA TCGAAACCCA CGAAGATCCC GATAATGCTC CGTCCGACGG CCCCAACATG GTGCCGATCG ACAAGATGCC GGCGCTTCTG GAAAAGCTGA TGGCATTCGA TCGCATTGCC AAGGCGCTGT AAAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA