BrabA.00097.a
Pantoate-beta-alanine ligase:Cytidyltransferase-related domain
| CENTER ID: | BrabA.00097.a |
| ORGANISM: | Brucella melitensis biovar Abortus 2308 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.00097.a.A1.GE25399 | Full length( BrabA.00097.a ) | 1 | 293 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.00097.a.A1.PS00055 | Full length( BrabA.00097.a ) | 1 | 293 | ||
| BrabA.00097.a.A1.PW25437 | Full length( BrabA.00097.a ) | 1 | 293 |
Structures
3INN
DEPOSITED: 8/12/2009
DETERMINATION: XRay
CLONE: BrabA.00097.a.A1.GE25399
PROTEIN: BrabA.00097.a.A1.PS00055
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_129569 |
| PATRIC ID: | fig|359391.4.peg.378 |
| RefSeq: | YP_413826 |
| UniProt: | Q2YPI2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQIIHTIEEL RQALAPARQQ GKKIGFVPTM GYLHKGHLEL VRRARVENDV TLVSIFVNPL QFGANEDLGR YPRDLERDAG LLHDAQVDYL FAPTVSDMYP RPMQTVVDVP PLGNQIEGEA RPGHFAGVAT VVSKLFNIVG PDAAYFGEKD FQQLVIIRRM VDDMAIPVRI VGVETVREDD GLACSSRNVY LTPEQRRAAI IVPQALDEAD RLYRSGMDDP DALEAAIRTF IGRQPLAVPE VIAIRDPETL ERLPALQGRP ILVALFVRVG ATRLLDNRVI GHAAPQITQE RAA
NT Sequence
ATGCAGATCA TCCACACCAT CGAGGAATTG CGCCAGGCGC TTGCGCCTGC CAGGCAGCAG GGCAAAAAGA TCGGCTTCGT GCCGACTATG GGTTATCTCC ATAAGGGCCA TCTGGAGCTT GTGCGCCGCG CGCGCGTGGA AAACGATGTG ACGCTGGTGT CGATCTTCGT CAACCCACTG CAATTTGGCG CGAATGAAGA CCTCGGCAGA TATCCCCGTG ATCTGGAGCG CGACGCCGGC CTTTTGCATG ACGCGCAGGT GGATTATCTT TTTGCGCCTA CGGTGAGCGA TATGTATCCT CGTCCCATGC AGACAGTGGT GGACGTGCCC CCGCTTGGCA ACCAGATAGA AGGTGAGGCG CGCCCCGGCC ATTTTGCCGG TGTTGCAACG GTGGTGAGCA AGCTATTCAA CATCGTCGGG CCGGATGCAG CCTATTTCGG CGAGAAGGAT TTCCAGCAGC TCGTCATCAT CCGCCGTATG GTGGACGACA TGGCCATTCC GGTGCGCATC GTCGGTGTTG AAACCGTGCG CGAGGATGAC GGGCTCGCCT GTTCGTCGCG CAATGTCTAC CTGACGCCCG AACAGCGCCG CGCCGCCATC ATCGTCCCGC AGGCGCTTGA TGAGGCTGAC CGGCTCTACC GTTCAGGCAT GGATGACCCG GATGCGCTGG AAGCAGCCAT TCGCACATTC ATCGGCAGGC AACCGCTGGC CGTGCCCGAA GTGATTGCGA TCCGCGATCC CGAAACGCTG GAGCGGCTGC CCGCCCTGCA GGGAAGGCCG ATCCTCGTTG CGCTTTTCGT GCGTGTGGGG GCGACGCGGC TTCTGGATAA TCGTGTGATC GGACATGCGG CGCCACAGAT CACACAGGAA AGGGCAGCC
Details for BrabA.00097.a.A1.GE25399
| HARVESTED ON: | 6/30/2008 |
| SEQUENCED ON: | 6/16/2009 |
| EXPECTED MW: | 34kDa |
| OBSERVED MW: | 35kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMQIIHTIEE LRQALAPARQ QGKKIGFVPT MGYLHKGHLE LVRRARVEND VTLVSIFVNP LQFGANEDLG RYPRDLERDA GLLHDAQVDY LFAPTVSDMY PRPMQTVVDV PPLGNQIEGE ARPGHFAGVA TVVSKLFNIV GPDAAYFGEK DFQQLVIIRR MVDDMAIPVR IVGVETVRED DGLACSSRNV YLTPEQRRAA IIVPXALDEA DRLYRSGMDD PDALEAAIRT FIGRQPLAVP EVIAIRDPET LERLPALQGX XILVALFVRV GATRLLXNRX IGXAA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcaga tcatccacac catcgaggaa ttgcgccagg cgcttgcgcc tgccaggcag cagggcaaaa agatcggctt cgtgccgact atgggttatc tccataaggg ccatctggag cttgtgcgcc gcgcgcgcgt ggaaaacgat gtgacgctgg tgtcgatctt cgtcaaccca ctgcaatttg gcgcgaatga agacctcggc agatatcccc gtgatctgga gcgcgacgcc ggccttttgc atgacgcgca ggtggattat ctttttgcgc ctacggtgag cgatatgtat cctcgtccca tgcagacagt ggtggacgtg cccccgcttg gcaaccagat agaaggtgag gcgcgccccg gccattttgc cggtgttgca acggtggtga gcaagctatt caacatcgtc gggccggatg cagcctattt cggcgagaag gatttccagc agctcgtcat catccgccgt atggtggacg acatggccat tccggtgcgc atcgtcggtg ttgaaaccgt gcgcgaggat gacgggctcg cctgttcgtc gcgcaatgtc tacctgacgc ccgaacagcg ccgcgccgcc atcatcgtcc cgcangcgct tgatgaggct gaccggctct accgttcagg catggatgac ccggatgcgc tggaagcagc cattcgcaca ttcatcggca ggcaaccgct ggccgtgccc gaagtgattg cgatccgcga tcccgaaacg ctggagcggc tgcccgccct gcagggaang ncgatcctcg ttgcgctttt cgtgcgtgtg ggggcgacgc ggcttctggn naatcgtgng atcggacntg cggcgc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQIIHTIEE LRQALAPARQ QGKKIGFVPT MGYLHKGHLE LVRRARVEND VTLVSIFVNP LQFGANEDLG RYPRDLERDA GLLHDAQVDY LFAPTVSDMY PRPMQTVVDV PPLGNQIEGE ARPGHFAGVA TVVSKLFNIV GPDAAYFGEK DFQQLVIIRR MVDDMAIPVR IVGVETVRED DGLACSSRNV YLTPEQRRAA IIVPQALDEA DRLYRSGMDD PDALEAAIRT FIGRQPLAVP EVIAIRDPET LERLPALQGR PILVALFVRV GATRLLDNRV IGHAAPQITQ ERAA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcagatcatc cacaccatcg aggaattgcg ccaggcgctt gcgcctgcca ggcagcaggg caaaaagatc ggcttcgtgc cgactatggg ttatctccat aagggccatc tggagcttgt gcgccgcgcg cgcgtggaaa acgatgtgac gctggtgtcg atcttcgtca acccactgca atttggcgcg aatgaagacc tcggcagata tccccgtgat ctggagcgcg acgccggcct tttgcatgac gcgcaggtgg attatctttt tgcgcctacg gtgagcgata tgtatcctcg tcccatgcag acagtggtgg acgtgccccc gcttggcaac cagatagaag gtgaggcgcg ccccggccat tttgccggtg ttgcaacggt ggtgagcaag ctattcaaca tcgtcgggcc ggatgcagcc tatttcggcg agaaggattt ccagcagctc gtcatcatcc gccgtatggt ggacgacatg gccattccgg tgcgcatcgt cggtgttgaa accgtgcgcg aggatgacgg gctcgcctgt tcgtcgcgca atgtctacct gacgcccgaa cagcgccgcg ccgccatcat cgtcccgcag gcgcttgatg aggctgaccg gctctaccgt tcaggcatgg atgacccgga tgcgctggaa gcagccattc gcacattcat cggcaggcaa ccgctggccg tgcccgaagt gattgcgatc cgcgatcccg aaacgctgga gcggctgccc gccctgcagg gaaggccgat cctcgttgcg cttttcgtgc gtgtgggggc gacgcggctt ctggataatc gtgtgatcgg acatgcggcg ccacagatca cacaggaaag ggcagcctga aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for BrabA.00097.a.A1.PS00055
| PURIFICATION DATe: | 8/12/2008 |
| CONCENTRATION: | 67.8mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 8 |
| VIAL VOLUME: | 150µl |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMQIIHTIEE LRQALAPARQ QGKKIGFVPT MGYLHKGHLE LVRRARVEND VTLVSIFVNP LQFGANEDLG RYPRDLERDA GLLHDAQVDY LFAPTVSDMY PRPMQTVVDV PPLGNQIEGE ARPGHFAGVA TVVSKLFNIV GPDAAYFGEK DFQQLVIIRR MVDDMAIPVR IVGVETVRED DGLACSSRNV YLTPEQRRAA IIVPXALDEA DRLYRSGMDD PDALEAAIRT FIGRQPLAVP EVIAIRDPET LERLPALQGX XILVALFVRV GATRLLXNRX IGXAA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcaga tcatccacac catcgaggaa ttgcgccagg cgcttgcgcc tgccaggcag cagggcaaaa agatcggctt cgtgccgact atgggttatc tccataaggg ccatctggag cttgtgcgcc gcgcgcgcgt ggaaaacgat gtgacgctgg tgtcgatctt cgtcaaccca ctgcaatttg gcgcgaatga agacctcggc agatatcccc gtgatctgga gcgcgacgcc ggccttttgc atgacgcgca ggtggattat ctttttgcgc ctacggtgag cgatatgtat cctcgtccca tgcagacagt ggtggacgtg cccccgcttg gcaaccagat agaaggtgag gcgcgccccg gccattttgc cggtgttgca acggtggtga gcaagctatt caacatcgtc gggccggatg cagcctattt cggcgagaag gatttccagc agctcgtcat catccgccgt atggtggacg acatggccat tccggtgcgc atcgtcggtg ttgaaaccgt gcgcgaggat gacgggctcg cctgttcgtc gcgcaatgtc tacctgacgc ccgaacagcg ccgcgccgcc atcatcgtcc cgcangcgct tgatgaggct gaccggctct accgttcagg catggatgac ccggatgcgc tggaagcagc cattcgcaca ttcatcggca ggcaaccgct ggccgtgccc gaagtgattg cgatccgcga tcccgaaacg ctggagcggc tgcccgccct gcagggaang ncgatcctcg ttgcgctttt cgtgcgtgtg ggggcgacgc ggcttctggn naatcgtgng atcggacntg cggcgc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQIIHTIEE LRQALAPARQ QGKKIGFVPT MGYLHKGHLE LVRRARVEND VTLVSIFVNP LQFGANEDLG RYPRDLERDA GLLHDAQVDY LFAPTVSDMY PRPMQTVVDV PPLGNQIEGE ARPGHFAGVA TVVSKLFNIV GPDAAYFGEK DFQQLVIIRR MVDDMAIPVR IVGVETVRED DGLACSSRNV YLTPEQRRAA IIVPQALDEA DRLYRSGMDD PDALEAAIRT FIGRQPLAVP EVIAIRDPET LERLPALQGR PILVALFVRV GATRLLDNRV IGHAAPQITQ ERAA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCAGATCATC CACACCATCG AGGAATTGCG CCAGGCGCTT GCGCCTGCCA GGCAGCAGGG CAAAAAGATC GGCTTCGTGC CGACTATGGG TTATCTCCAT AAGGGCCATC TGGAGCTTGT GCGCCGCGCG CGCGTGGAAA ACGATGTGAC GCTGGTGTCG ATCTTCGTCA ACCCACTGCA ATTTGGCGCG AATGAAGACC TCGGCAGATA TCCCCGTGAT CTGGAGCGCG ACGCCGGCCT TTTGCATGAC GCGCAGGTGG ATTATCTTTT TGCGCCTACG GTGAGCGATA TGTATCCTCG TCCCATGCAG ACAGTGGTGG ACGTGCCCCC GCTTGGCAAC CAGATAGAAG GTGAGGCGCG CCCCGGCCAT TTTGCCGGTG TTGCAACGGT GGTGAGCAAG CTATTCAACA TCGTCGGGCC GGATGCAGCC TATTTCGGCG AGAAGGATTT CCAGCAGCTC GTCATCATCC GCCGTATGGT GGACGACATG GCCATTCCGG TGCGCATCGT CGGTGTTGAA ACCGTGCGCG AGGATGACGG GCTCGCCTGT TCGTCGCGCA ATGTCTACCT GACGCCCGAA CAGCGCCGCG CCGCCATCAT CGTCCCGCAG GCGCTTGATG AGGCTGACCG GCTCTACCGT TCAGGCATGG ATGACCCGGA TGCGCTGGAA GCAGCCATTC GCACATTCAT CGGCAGGCAA CCGCTGGCCG TGCCCGAAGT GATTGCGATC CGCGATCCCG AAACGCTGGA GCGGCTGCCC GCCCTGCAGG GAAGGCCGAT CCTCGTTGCG CTTTTCGTGC GTGTGGGGGC GACGCGGCTT CTGGATAATC GTGTGATCGG ACATGCGGCG CCACAGATCA CACAGGAAAG GGCAGCCTGA AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA
Details for BrabA.00097.a.A1.PW25437
| PURIFICATION DATe: | 7/14/2009 |
| CONCENTRATION: | 28.02mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 14 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMQIIHTIEE LRQALAPARQ QGKKIGFVPT MGYLHKGHLE LVRRARVEND VTLVSIFVNP LQFGANEDLG RYPRDLERDA GLLHDAQVDY LFAPTVSDMY PRPMQTVVDV PPLGNQIEGE ARPGHFAGVA TVVSKLFNIV GPDAAYFGEK DFQQLVIIRR MVDDMAIPVR IVGVETVRED DGLACSSRNV YLTPEQRRAA IIVPXALDEA DRLYRSGMDD PDALEAAIRT FIGRQPLAVP EVIAIRDPET LERLPALQGX XILVALFVRV GATRLLXNRX IGXAA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcaga tcatccacac catcgaggaa ttgcgccagg cgcttgcgcc tgccaggcag cagggcaaaa agatcggctt cgtgccgact atgggttatc tccataaggg ccatctggag cttgtgcgcc gcgcgcgcgt ggaaaacgat gtgacgctgg tgtcgatctt cgtcaaccca ctgcaatttg gcgcgaatga agacctcggc agatatcccc gtgatctgga gcgcgacgcc ggccttttgc atgacgcgca ggtggattat ctttttgcgc ctacggtgag cgatatgtat cctcgtccca tgcagacagt ggtggacgtg cccccgcttg gcaaccagat agaaggtgag gcgcgccccg gccattttgc cggtgttgca acggtggtga gcaagctatt caacatcgtc gggccggatg cagcctattt cggcgagaag gatttccagc agctcgtcat catccgccgt atggtggacg acatggccat tccggtgcgc atcgtcggtg ttgaaaccgt gcgcgaggat gacgggctcg cctgttcgtc gcgcaatgtc tacctgacgc ccgaacagcg ccgcgccgcc atcatcgtcc cgcangcgct tgatgaggct gaccggctct accgttcagg catggatgac ccggatgcgc tggaagcagc cattcgcaca ttcatcggca ggcaaccgct ggccgtgccc gaagtgattg cgatccgcga tcccgaaacg ctggagcggc tgcccgccct gcagggaang ncgatcctcg ttgcgctttt cgtgcgtgtg ggggcgacgc ggcttctggn naatcgtgng atcggacntg cggcgc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQIIHTIEE LRQALAPARQ QGKKIGFVPT MGYLHKGHLE LVRRARVEND VTLVSIFVNP LQFGANEDLG RYPRDLERDA GLLHDAQVDY LFAPTVSDMY PRPMQTVVDV PPLGNQIEGE ARPGHFAGVA TVVSKLFNIV GPDAAYFGEK DFQQLVIIRR MVDDMAIPVR IVGVETVRED DGLACSSRNV YLTPEQRRAA IIVPQALDEA DRLYRSGMDD PDALEAAIRT FIGRQPLAVP EVIAIRDPET LERLPALQGR PILVALFVRV GATRLLDNRV IGHAAPQITQ ERAA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCAGATCATC CACACCATCG AGGAATTGCG CCAGGCGCTT GCGCCTGCCA GGCAGCAGGG CAAAAAGATC GGCTTCGTGC CGACTATGGG TTATCTCCAT AAGGGCCATC TGGAGCTTGT GCGCCGCGCG CGCGTGGAAA ACGATGTGAC GCTGGTGTCG ATCTTCGTCA ACCCACTGCA ATTTGGCGCG AATGAAGACC TCGGCAGATA TCCCCGTGAT CTGGAGCGCG ACGCCGGCCT TTTGCATGAC GCGCAGGTGG ATTATCTTTT TGCGCCTACG GTGAGCGATA TGTATCCTCG TCCCATGCAG ACAGTGGTGG ACGTGCCCCC GCTTGGCAAC CAGATAGAAG GTGAGGCGCG CCCCGGCCAT TTTGCCGGTG TTGCAACGGT GGTGAGCAAG CTATTCAACA TCGTCGGGCC GGATGCAGCC TATTTCGGCG AGAAGGATTT CCAGCAGCTC GTCATCATCC GCCGTATGGT GGACGACATG GCCATTCCGG TGCGCATCGT CGGTGTTGAA ACCGTGCGCG AGGATGACGG GCTCGCCTGT TCGTCGCGCA ATGTCTACCT GACGCCCGAA CAGCGCCGCG CCGCCATCAT CGTCCCGCAG GCGCTTGATG AGGCTGACCG GCTCTACCGT TCAGGCATGG ATGACCCGGA TGCGCTGGAA GCAGCCATTC GCACATTCAT CGGCAGGCAA CCGCTGGCCG TGCCCGAAGT GATTGCGATC CGCGATCCCG AAACGCTGGA GCGGCTGCCC GCCCTGCAGG GAAGGCCGAT CCTCGTTGCG CTTTTCGTGC GTGTGGGGGC GACGCGGCTT CTGGATAATC GTGTGATCGG ACATGCGGCG CCACAGATCA CACAGGAAAG GGCAGCCTGA AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA