SSGCID: Target Page

BrabA.00089.b

Bacterial extracellular solute-binding protein, family 1

CENTER ID:	BrabA.00089.b
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.00089.b.A2.GE30879	Mature protein( BrabA.00089.b )		25	348

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.00089.b.A2.PW31745	Mature protein( BrabA.00089.b )		25	348

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|359391.4.peg.3170
RefSeq:	YP_419031.1
UniProt:	Q2YK03

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKFARLALMG GIFATVAFTV GPAFARDLTV ASWGGNYQDA QREIYFKPFA EKTGKPLLDE SWDGGYGVIQ SKVKAGSPNW DVVQVEAEEL ALGCADGLYE KIDWDKVGGK DKFLDSAVND CGVGAIVWST AIAYNGDKLK DGPKSWADFW DVKKFPGKRS LRKSAKYTLE FALMADGVDK DDVYDVLSTP EGVDRAFKKL DELKPHIVWW EAGAQPLQLL ASDEVVMASA YNGRITGINR SEGKNFKVVW PGSIYAVDSW VILKGAENKD AGLDFIAFAS EPEHQVKLPK YVAYGLPNKE AAAKVPEEYA ADLPTAKANM KDALALDVDF WIDHSEELTK RFNAWLAQ

NT Sequence

ATGAAGTTTG CTCGCCTTGC CCTTATGGGC GGAATTTTCG CTACCGTTGC CTTCACCGTC GGCCCCGCCT TCGCGCGCGA CCTTACCGTC GCGTCATGGG GCGGCAACTA TCAGGATGCG CAGCGCGAAA TCTATTTCAA ACCTTTTGCG GAAAAGACCG GCAAGCCGCT GCTCGATGAA TCATGGGATG GCGGTTACGG GGTCATCCAG TCAAAAGTGA AGGCCGGCTC GCCGAATTGG GACGTGGTTC AGGTCGAGGC GGAGGAGCTG GCGCTCGGTT GCGCCGATGG TCTTTATGAA AAGATCGACT GGGACAAGGT GGGCGGCAAG GACAAATTCC TCGACAGTGC GGTCAATGAT TGCGGGGTTG GCGCCATCGT CTGGTCCACT GCCATAGCTT ATAATGGCGA CAAGCTGAAG GATGGGCCGA AATCCTGGGC GGATTTCTGG GATGTGAAGA AGTTTCCGGG CAAGCGATCG CTGCGCAAGA GTGCGAAATA CACGCTGGAA TTCGCGCTTA TGGCTGATGG CGTCGACAAG GATGACGTTT ATGACGTCCT TTCCACGCCG GAAGGCGTGG ACCGCGCCTT CAAGAAGCTC GATGAGCTGA AGCCACATAT CGTGTGGTGG GAAGCCGGTG CGCAGCCCTT GCAATTGCTG GCGTCGGACG AGGTGGTGAT GGCATCGGCT TATAATGGCC GCATCACTGG CATCAACCGT TCCGAAGGCA AGAATTTCAA GGTCGTCTGG CCGGGCAGCA TCTATGCGGT GGATAGCTGG GTGATTCTGA AGGGGGCTGA AAATAAGGAT GCGGGCCTGG ATTTCATTGC CTTTGCCAGT GAGCCCGAAC ATCAGGTCAA GCTGCCGAAA TATGTTGCCT ACGGCCTGCC GAACAAGGAA GCGGCAGCCA AGGTGCCGGA GGAATATGCA GCAGACCTGC CCACGGCCAA AGCAAACATG AAGGATGCCC TCGCGCTCGA TGTTGATTTC TGGATCGACC ATTCAGAAGA GCTGACGAAG CGTTTCAACG CCTGGCTGGC GCAA

Details for BrabA.00089.b.A2.GE30879

HARVESTED ON:	11/16/2010
SEQUENCED ON:	11/23/2010
EXPECTED MW:	38kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	93

Validated AA Sequence

MARDLTVASW GGNYQDAQRE IYFKPFAEKT GKPLLDESWD GGYGVIQSKV KAGSPNWDVV QVEAEELALG CADGLYEKID WDKVGGKDKF LDSAVNDCGV GAIVWSTAIA YNGDKLKDGP KSWADFWDVK KFPGKRSLRK SAKYTLEFAL MADGVDKDDV YDVLSTPEGV DRAFKKLDEL KPHIVWWEAG AQPLQLLASD EVVMASAYNG RITGINRSEG KNFKVVCPGS IYAVDSWVIL KGAENKDAGL DFIAFASEPE HQVKLPKYVA YGLPNKEAAA KVPEEYAADL PTAKANMKDA LALDVDFWID HSEELTKRFN AWLAQ

Validated NT Sequence

tattattgcg ccagccaggc gttgaaacgc ttcgtcagct cttctgaatg gtcgatccag aaatcaacat cgagcgcgag ggcatccttc atgtttgctt tggccgtggg caggtctgct gcatattcct ccggcacctt ggctgccgct tccttgttcg gcaggccgta ggcaacatat ttcggcagct tgacctgatg ttcgggctca ctggcaaagg caatgaaatc caggcccgca tccttatttt cagccccctt cagaatcacc cagctatcca ccgcatagat gctgcccgga cagacgacct tgaaattctt gccttcggaa cggttgatgc cagtgatgcg gccattataa gccgatgcca tcaccacctc gtccgacgcc agcaattgca agggctgcgc accggcttcc caccacacga tatgtggctt cagctcatcg agcttcttga aggcgcggtc cacgccttcc ggcgtggaaa ggacgtcata aacgtcatcc ttgtcgacgc catcagccat aagcgcgaat tccagcgtgt atttcgcact cttgcgcagc gatcgcttgc ccggaaactt cttcacatcc cagaaatccg cccaggattt cggcccatcc ttcagcttgt cgccattata agctatggca gtggaccaga cgatggcgcc aaccccgcaa tcattgaccg cactgtcgag gaatttgtcc ttgccgccca ccttgtccca gtcgatcttt tcataaagac catcggcgca accgagcgcc agctcctccg cctcgacctg aaccacgtcc caattcggcg agccggcctt cacttttgac tggatgaccc cgtaaccgcc atcccatgat tcatcgagca gcggcttgcc ggtcttttcc gcaaaaggtt tgaaatagat ttcgcgctgc gcatcctgat agttgccgcc ccatgacgcg acggtaaggt cgcgcgccat cgaaccagga ccctgggtct gagcttccag

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMARDLTVAS WGGNYQDAQR EIYFKPFAEK TGKPLLDESW DGGYGVIQSK VKAGSPNWDV VQVEAEELAL GCADGLYEKI DWDKVGGKDK FLDSAVNDCG VGAIVWSTAI AYNGDKLKDG PKSWADFWDV KKFPGKRSLR KSAKYTLEFA LMADGVDKDD VYDVLSTPEG VDRAFKKLDE LKPHIVWWEA GAQPLQLLAS DEVVMASAYN GRITGINRSE GKNFKVVWPG SIYAVDSWVI LKGAENKDAG LDFIAFASEP EHQVKLPKYV AYGLPNKEAA AKVPEEYAAD LPTAKANMKD ALALDVDFWI DHSEELTKRF NAWLAQ

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgcgcgac cttaccgtcg cgtcatgggg cggcaactat caggatgcgc agcgcgaaat ctatttcaaa ccttttgcgg aaaagaccgg caagccgctg ctcgatgaat catgggatgg cggttacggg gtcatccagt caaaagtgaa ggccggctcg ccgaattggg acgtggttca ggtcgaggcg gaggagctgg cgctcggttg cgccgatggt ctttatgaaa agatcgactg ggacaaggtg ggcggcaagg acaaattcct cgacagtgcg gtcaatgatt gcggggttgg cgccatcgtc tggtccactg ccatagctta taatggcgac aagctgaagg atgggccgaa atcctgggcg gatttctggg atgtgaagaa gtttccgggc aagcgatcgc tgcgcaagag tgcgaaatac acgctggaat tcgcgcttat ggctgatggc gtcgacaagg atgacgttta tgacgtcctt tccacgccgg aaggcgtgga ccgcgccttc aagaagctcg atgagctgaa gccacatatc gtgtggtggg aagccggtgc gcagcccttg caattgctgg cgtcggacga ggtggtgatg gcatcggctt ataatggccg catcactggc atcaaccgtt ccgaaggcaa gaatttcaag gtcgtctggc cgggcagcat ctatgcggtg gatagctggg tgattctgaa gggggctgaa aataaggatg cgggcctgga tttcattgcc tttgccagtg agcccgaaca tcaggtcaag ctgccgaaat atgttgccta cggcctgccg aacaaggaag cggcagccaa ggtgccggag gaatatgcag cagacctgcc cacggccaaa gcaaacatga aggatgccct cgcgctcgat gttgatttct ggatcgacca ttcagaagag ctgacgaagc gtttcaacgc ctggctggcg caataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa

Details for BrabA.00089.b.A2.PW31745

PURIFICATION DATe:	2/18/2011
CONCENTRATION:	39.32mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	9
VIAL VOLUME:	100µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	93

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGCGCGAC CTTACCGTCG CGTCATGGGG CGGCAACTAT CAGGATGCGC AGCGCGAAAT CTATTTCAAA CCTTTTGCGG AAAAGACCGG CAAGCCGCTG CTCGATGAAT CATGGGATGG CGGTTACGGG GTCATCCAGT CAAAAGTGAA GGCCGGCTCG CCGAATTGGG ACGTGGTTCA GGTCGAGGCG GAGGAGCTGG CGCTCGGTTG CGCCGATGGT CTTTATGAAA AGATCGACTG GGACAAGGTG GGCGGCAAGG ACAAATTCCT CGACAGTGCG GTCAATGATT GCGGGGTTGG CGCCATCGTC TGGTCCACTG CCATAGCTTA TAATGGCGAC AAGCTGAAGG ATGGGCCGAA ATCCTGGGCG GATTTCTGGG ATGTGAAGAA GTTTCCGGGC AAGCGATCGC TGCGCAAGAG TGCGAAATAC ACGCTGGAAT TCGCGCTTAT GGCTGATGGC GTCGACAAGG ATGACGTTTA TGACGTCCTT TCCACGCCGG AAGGCGTGGA CCGCGCCTTC AAGAAGCTCG ATGAGCTGAA GCCACATATC GTGTGGTGGG AAGCCGGTGC GCAGCCCTTG CAATTGCTGG CGTCGGACGA GGTGGTGATG GCATCGGCTT ATAATGGCCG CATCACTGGC ATCAACCGTT CCGAAGGCAA GAATTTCAAG GTCGTCTGGC CGGGCAGCAT CTATGCGGTG GATAGCTGGG TGATTCTGAA GGGGGCTGAA AATAAGGATG CGGGCCTGGA TTTCATTGCC TTTGCCAGTG AGCCCGAACA TCAGGTCAAG CTGCCGAAAT ATGTTGCCTA CGGCCTGCCG AACAAGGAAG CGGCAGCCAA GGTGCCGGAG GAATATGCAG CAGACCTGCC CACGGCCAAA GCAAACATGA AGGATGCCCT CGCGCTCGAT GTTGATTTCT GGATCGACCA TTCAGAAGAG CTGACGAAGC GTTTCAACGC CTGGCTGGCG CAATAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA