BrabA.00089.a

Bacterial periplasmic spermidine/putrescine-binding protein:Bacterial extracellular solute-binding protein, family 1

CENTER ID: BrabA.00089.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BrabA.00089.a.A3.GE28050 Created from Decode construct for feature BrabA.00089.a 1 365
BrabA.00089.a.A4.GE28051 Created from Decode construct for feature BrabA.00089.a 25 367
BrabA.00089.a.A5.GE28052 Created from Decode construct for feature BrabA.00089.a 25 365
BrabA.00089.a.A6.GE28053 Created from Decode construct for feature BrabA.00089.a 29 367
BrabA.00089.a.A7.GE28054 Created from Decode construct for feature BrabA.00089.a 29 365
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BrabA.00089.a.A4.PS00950 Created from Decode construct for feature BrabA.00089.a 25 367
BrabA.00089.a.A3.PW27943 Created from Decode construct for feature BrabA.00089.a 1 365
BrabA.00089.a.A5.PW27945 Created from Decode construct for feature BrabA.00089.a 25 365
BrabA.00089.a.A6.PW27946 Created from Decode construct for feature BrabA.00089.a 29 367
BrabA.00089.a.A7.PW27947 Created from Decode construct for feature BrabA.00089.a 29 365
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_131894
PATRIC ID: fig|359391.4.peg.1686
RefSeq: YP_414988
UniProt: Q2YQA1
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGIKSFLLAT TVATGFVAAA TFSAGAQERV VNIYNWSDYI DDSILKDFTK ETGIKVVYDV YDSNEILETK LLAGGNGYDL VVPSGEFLGR QIPAGVFLKL DKDKLPNLKN MWDEISTRAA TYDPGNEYSV NYMWGTTGIG YNKAKIKEAL GTDTIDSWDV LFDPEKTAKL KDCGIYLLDS ASEMLRPALN YLGLDPNSPS PDDLQKAQDL YLKIRPNIRK FHSSEYINAL ANGDICMAVG YSGDIFQARD RAEKAKQGVE IGYSIPKEGA LIWFDQMAIP ADAKHVPEAL EFMNYMMRPE VAAKASNYVF YANGNKASQK FIDKEILDDP EIYPSDEVMK KLFVPTPYDT KTQRVVTRAW TKIVTGQ
NT Sequence
ATGGGGATCA AATCCTTCCT TCTGGCAACG ACCGTTGCGA CGGGTTTTGT CGCGGCTGCC ACATTTTCCG CAGGCGCGCA GGAGCGGGTG GTCAATATCT ATAACTGGTC GGATTATATC GACGATTCCA TCCTCAAGGA CTTCACCAAG GAGACCGGGA TCAAGGTCGT CTACGACGTC TATGACTCCA ACGAAATTCT GGAAACCAAG CTTCTGGCGG GCGGCAACGG CTACGACCTC GTGGTGCCAT CGGGCGAATT TCTTGGGCGC CAGATTCCCG CAGGCGTGTT CCTGAAACTC GACAAGGACA AGCTGCCGAA CCTCAAGAAT ATGTGGGATG AGATTTCGAC CCGTGCGGCA ACCTATGATC CGGGCAACGA ATATTCCGTC AATTACATGT GGGGCACGAC CGGCATCGGC TACAATAAGG CCAAGATCAA GGAAGCGCTC GGCACCGACA CGATCGACTC CTGGGACGTG CTTTTCGATC CGGAAAAAAC GGCAAAGCTG AAAGATTGCG GCATTTACCT GCTTGATTCC GCCAGCGAAA TGCTGCGTCC GGCGCTGAAC TATCTGGGTC TCGACCCGAA CTCTCCGTCG CCGGACGATT TGCAGAAGGC ACAGGATCTC TATCTCAAGA TTCGTCCGAA TATCCGCAAA TTCCACTCGT CGGAATATAT CAATGCGCTC GCCAATGGCG ATATCTGCAT GGCTGTCGGC TATTCCGGCG ATATTTTCCA GGCCCGCGAC CGCGCCGAAA AGGCGAAGCA GGGGGTGGAG ATCGGCTATT CGATCCCGAA GGAAGGCGCG CTGATCTGGT TTGACCAGAT GGCGATCCCG GCCGATGCCA AGCATGTGCC GGAGGCGTTG GAATTCATGA ATTATATGAT GCGCCCGGAG GTGGCGGCAA AGGCGTCGAA CTATGTGTTC TATGCCAATG GCAACAAGGC GTCGCAGAAA TTCATCGACA AGGAAATCCT CGACGACCCG GAAATCTATC CGTCCGACGA GGTGATGAAG AAGCTGTTCG TGCCGACGCC ATATGACACG AAGACCCAGC GTGTGGTCAC ACGCGCCTGG ACCAAGATCG TCACCGGCCA G
Details for BrabA.00089.a.A3.GE28050
HARVESTED ON: 12/7/2009
SEQUENCED ON: 12/23/2009
EXPECTED MW: 43kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 68
Validated AA Sequence
MGTLEAQTQG PGSMGIKSFL LATTVATGFV AAATFSAGAQ ERVVNIYNWS DYIDDSILKD FTKETGIKVV YDVYDSNEIL ETKLLAGGNG YDLVVPSGEF LGRQIPAGVF LKLDKDKLPN LKNMWDEIST RAATYDPGNE YSVNYMWGTT GIGYNKAKIK EALGTDTIDS WDVLFDPEKT AKLKDCGIYL LDSASEMLRP ALNYLGLDPN SPSPXDLQXX QDLYLKIRPN IRKFHSSEYI NALAXGDXCM AVGYSGDIFQ ARDR
Validated NT Sequence
tcaccatcat atgggtaccc tggaagctca gacccagggt cctggttcga tggggatcaa atccttcctt ctggcaacga ccgttgcgac gggttttgtc gcggctgcca cattttccgc aggcgcgcag gagcgggtgg tcaatatcta taactggtcg gattatatcg acgattccat cctcaaggac ttcaccaagg agaccgggat caaggtcgtc tacgacgtct atgactccaa cgaaattctg gaaaccaagc ttctggcggg cggcaacggc tacgacctcg tggtgccatc gggcgaattt cttgggcgcc agattcccgc aggcgtgttc ctgaaactcg acaaggacaa gctgccgaac ctcaagaata tgtgggatga gatttcgacc cgtgcggcaa cctatgatcc gggcaacgaa tattccgtca attacatgtg gggcacgacc ggcatcggct acaataaggc caagatcaag gaagcgctcg gcaccgacac gatcgactcc tgggacgtgc ttttcgatcc ggaaaaaacg gcaaagctga aagattgcgg catttacctg cttgattccg ccagcgaaat gctgcgtccg gcgctgaact atctgggtct cgacccgaac tctccgtcgc cggangattt gcagaangna caggatctct atctcaagat tcgtccgaat atccgcaaat tccactcgtc ngaatatatc aatgcgctcg ccnatggcga tntctgcatg gctgtcggct attccggcga tattttccag gcccgcgacc gcgcc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGIKSFLLA TTVATGFVAA ATFSAGAQER VVNIYNWSDY IDDSILKDFT KETGIKVVYD VYDSNEILET KLLAGGNGYD LVVPSGEFLG RQIPAGVFLK LDKDKLPNLK NMWDEISTRA ATYDPGNEYS VNYMWGTTGI GYNKAKIKEA LGTDTIDSWD VLFDPEKTAK LKDCGIYLLD SASEMLRPAL NYLGLDPNSP SPDDLQKAQD LYLKIRPNIR KFHSSEYINA LANGDICMAV GYSGDIFQAR DRAEKAKQGV EIGYSIPKEG ALIWFDQMAI PADAKHVPEA LEFMNYMMRP EVAAKASNYV FYANGNKASQ KFIDKEILDD PEIYPSDEVM KKLFVPTPYD TKTQRVVTRA WTKIVT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggggatcaaa tccttccttc tggcaacgac cgttgcgacg ggttttgtcg cggctgccac attttccgca ggcgcgcagg agcgggtggt caatatctat aactggtcgg attatatcga cgattccatc ctcaaggact tcaccaagga gaccgggatc aaggtcgtct acgacgtcta tgactccaac gaaattctgg aaaccaagct tctggcgggc ggcaacggct acgacctcgt ggtgccatcg ggcgaatttc ttgggcgcca gattcccgca ggcgtgttcc tgaaactcga caaggacaag ctgccgaacc tcaagaatat gtgggatgag atttcgaccc gtgcggcaac ctatgatccg ggcaacgaat attccgtcaa ttacatgtgg ggcacgaccg gcatcggcta caataaggcc aagatcaagg aagcgctcgg caccgacacg atcgactcct gggacgtgct tttcgatccg gaaaaaacgg caaagctgaa agattgcggc atttacctgc ttgattccgc cagcgaaatg ctgcgtccgg cgctgaacta tctgggtctc gacccgaact ctccgtcgcc ggacgatttg cagaaggcac aggatctcta tctcaagatt cgtccgaata tccgcaaatt ccactcgtcg gaatatatca atgcgctcgc caatggcgat atctgcatgg ctgtcggcta ttccggcgat attttccagg cccgcgaccg cgccgaaaag gcgaagcagg gggtggagat cggctattcg atcccgaagg aaggcgcgct gatctggttt gaccagatgg cgatcccggc cgatgccaag catgtgccgg aggcgttgga attcatgaat tatatgatgc gcccggaggt ggcggcaaag gcgtcgaact atgtgttcta tgccaatggc aacaaggcgt cgcagaaatt catcgacaag gaaatcctcg acgacccgga aatctatccg tccgacgagg tgatgaagaa gctgttcgtg ccgacgccat atgacacgaa gacccagcgt gtggtcacac gcgcctggac caagatcgtc accaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for BrabA.00089.a.A4.GE28051
HARVESTED ON: 12/7/2009
SEQUENCED ON: 2/24/2010
EXPECTED MW: 41kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 92
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGAQERVVN IYNWSDYIDD SILKDFTKET GIKVVYDVYD SNEILETKLL AGGNGYDLVV PSGEFLGRQI PAGVFLKLDK DKLPNLKNMW DEISTRAATY DPGNEYSVNY MWGTTGIGYN KAKIKEALGT DTIDSWDVLF DPEKTAKLKD CGIYLLDSAS EMLRPALNYL GLDPNSPSPD DLQKAQDLYL KIRPNIRKFH SSEYINALAN GDICMAVGYS GDIFQARDRA EKAKQGVEIG YSIPKEGALI WFDQMAIPAD AKHVPEALEF MNYMMRPEVA AKASNYVFYA NGNKASXKFI DKEIXDDPEI YPSXXX
Validated NT Sequence
aattttgntt nnctttaaga aggagatata ccatggctca tcaccatcac catcatatgg gtaccctgga agctcagacc cagggtcctg gttcgatggg cgcgcaggag cgggtggtca atatctataa ctggtcggat tatatcgacg attccatcct caaggacttc accaaggaga ccgggatcaa ggtcgtctac gacgtctatg actccaacga aattctggaa accaagcttc tggcgggcgg caacggctac gacctcgtgg tgccatcggg cgaatttctt gggcgccaga ttcccgcagg cgtgttcctg aaactcgaca aggacaagct gccgaacctc aagaatatgt gggatgagat ttcgacccgt gcggcaacct atgatccggg caacgaatat tccgtcaatt acatgtgggg cacgaccggc atcggctaca ataaggccaa gatcaaggaa gcgctcggca ccgacacgat cgactcctgg gacgtgcttt tcgatccgga aaaaacggca aagctgaaag attgcggcat ttacctgctt gattccgcca gcgaaatgct gcgtccggcg ctgaactatc tgggtctcga cccgaactct ccgtcgccgg acgatttgca gaaggcacag gatctctatc tcaagattcg tccgaatatc cgcaaattcc actcgtcgga atatatcaat gcgctcgcca atggcgatat ctgcatggct gtcggctatt ccggcgatat tttccaggcc cgcgaccgcg ccgaaaaggc gaagcagggg gtggagatcg gctattcgat cccgaaggaa ggcgcgctga tctggtttga ccagatggcg atcccggccg atgccaagca tgtgccggag gcgttggaat tcatgaatta tatgatgcgc ccggaggtgg cggcaaaggc gtcnaactat gtgttctatg ccaatggcaa caaggcgtcg canaaattca tcgacaagga aatccncgac gacccngaaa tctatccgtc cgannannna tgananctnn tcntgccnnc ncntatgann cnanacccnn gnnnncncnn ncnncnggnc nannatcgnc nnnnnntann naacnannna ntnntgcann nnntnnnnnn ngnnnnnnnn nancnnannt nnnnnnnnnn ncncnngnnn nnnngnnnnn nnnnnnnngg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGAQERVVN IYNWSDYIDD SILKDFTKET GIKVVYDVYD SNEILETKLL AGGNGYDLVV PSGEFLGRQI PAGVFLKLDK DKLPNLKNMW DEISTRAATY DPGNEYSVNY MWGTTGIGYN KAKIKEALGT DTIDSWDVLF DPEKTAKLKD CGIYLLDSAS EMLRPALNYL GLDPNSPSPD DLQKAQDLYL KIRPNIRKFH SSEYINALAN GDICMAVGYS GDIFQARDRA EKAKQGVEIG YSIPKEGALI WFDQMAIPAD AKHVPEALEF MNYMMRPEVA AKASNYVFYA NGNKASQKFI DKEILDDPEI YPSDEVMKKL FVPTPYDTKT QRVVTRAWTK IVTGQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggcgcgcag gagcgggtgg tcaatatcta taactggtcg gattatatcg acgattccat cctcaaggac ttcaccaagg agaccgggat caaggtcgtc tacgacgtct atgactccaa cgaaattctg gaaaccaagc ttctggcggg cggcaacggc tacgacctcg tggtgccatc gggcgaattt cttgggcgcc agattcccgc aggcgtgttc ctgaaactcg acaaggacaa gctgccgaac ctcaagaata tgtgggatga gatttcgacc cgtgcggcaa cctatgatcc gggcaacgaa tattccgtca attacatgtg gggcacgacc ggcatcggct acaataaggc caagatcaag gaagcgctcg gcaccgacac gatcgactcc tgggacgtgc ttttcgatcc ggaaaaaacg gcaaagctga aagattgcgg catttacctg cttgattccg ccagcgaaat gctgcgtccg gcgctgaact atctgggtct cgacccgaac tctccgtcgc cggacgattt gcagaaggca caggatctct atctcaagat tcgtccgaat atccgcaaat tccactcgtc ggaatatatc aatgcgctcg ccaatggcga tatctgcatg gctgtcggct attccggcga tattttccag gcccgcgacc gcgccgaaaa ggcgaagcag ggggtggaga tcggctattc gatcccgaag gaaggcgcgc tgatctggtt tgaccagatg gcgatcccgg ccgatgccaa gcatgtgccg gaggcgttgg aattcatgaa ttatatgatg cgcccggagg tggcggcaaa ggcgtcgaac tatgtgttct atgccaatgg caacaaggcg tcgcagaaat tcatcgacaa ggaaatcctc gacgacccgg aaatctatcc gtccgacgag gtgatgaaga agctgttcgt gccgacgcca tatgacacga agacccagcg tgtggtcaca cgcgcctgga ccaagatcgt caccggccag aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for BrabA.00089.a.A5.GE28052
HARVESTED ON: 12/7/2009
SEQUENCED ON: 12/23/2009
EXPECTED MW: 40kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 85
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGAQERVVN IYNWSDYIDD SILKDFTKET GIKVVYDVYD SNEILETKLL AGGNGYDLVV PSGEFLGRQI PAGVFLKLDK DKLPNLKNMW DEISTRAATY DPGNEYSVNY MWGTTGIGYN KAKIKEALGT DTIDSWDVLF DPEKTAKLKD CGIYLLDSAS EMLRPALNYL GLDPNSPSPD DLQKAQDLYL KIRPNIRKFH SSEYINALAN GDICMAVGYS GDIFQARDRA EKAKQGVEIG YSIPKEGALI WFDQMAIPAD AKHVPXALEF MNYMMRPEVA AKASNYVFY
Validated NT Sequence
tttaagaagg aganntacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgggcgc gcaggagcgg gtggtcaata tctataactg gtcggattat atcgacgatt ccatcctcaa ggacttcacc aaggagaccg ggatcaaggt cgtctacgac gtctatgact ccaacgaaat tctggaaacc aagcttctgg cgggcggcaa cggctacgac ctcgtggtgc catcgggcga atttcttggg cgccagattc ccgcaggcgt gttcctgaaa ctcgacaagg acaagctgcc gaacctcaag aatatgtggg atgagatttc gacccgtgcg gcaacctatg atccgggcaa cgaatattcc gtcaattaca tgtggggcac gaccggcatc ggctacaata aggccaagat caaggaagcg ctcggcaccg acacgatcga ctcctgggac gtgcttttcg atccggaaaa aacggcaaag ctgaaagatt gcggcattta cctgcttgat tccgccagcg aaatgctgcg tccggcgctg aactatctgg gtctcgaccc gaactctccg tcgccggacg atttgcagaa ggcacaggat ctctatctca agattcgtcc gaatatccgc aaattccact cgtcggaata tatcaatgcg ctcgccaatg gcgatatctg catggctgtc ggctattccg gcgatatttt ccaggcccgc gaccgcgccg aaaaggcgaa gcagggggtg gagatcggct attcgatccc gaaggaaggc gcgctgatct ggtttgacca gatggcgatc ccggccgatg ccaagcatgt gccggangcg ttggaattca tgaattatat gatgcgcccg gaggtggcgg caaaggcgtc gaactatgtg ttctatgnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGAQERVVN IYNWSDYIDD SILKDFTKET GIKVVYDVYD SNEILETKLL AGGNGYDLVV PSGEFLGRQI PAGVFLKLDK DKLPNLKNMW DEISTRAATY DPGNEYSVNY MWGTTGIGYN KAKIKEALGT DTIDSWDVLF DPEKTAKLKD CGIYLLDSAS EMLRPALNYL GLDPNSPSPD DLQKAQDLYL KIRPNIRKFH SSEYINALAN GDICMAVGYS GDIFQARDRA EKAKQGVEIG YSIPKEGALI WFDQMAIPAD AKHVPEALEF MNYMMRPEVA AKASNYVFYA NGNKASQKFI DKEILDDPEI YPSDEVMKKL FVPTPYDTKT QRVVTRAWTK IVT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggcgcgcag gagcgggtgg tcaatatcta taactggtcg gattatatcg acgattccat cctcaaggac ttcaccaagg agaccgggat caaggtcgtc tacgacgtct atgactccaa cgaaattctg gaaaccaagc ttctggcggg cggcaacggc tacgacctcg tggtgccatc gggcgaattt cttgggcgcc agattcccgc aggcgtgttc ctgaaactcg acaaggacaa gctgccgaac ctcaagaata tgtgggatga gatttcgacc cgtgcggcaa cctatgatcc gggcaacgaa tattccgtca attacatgtg gggcacgacc ggcatcggct acaataaggc caagatcaag gaagcgctcg gcaccgacac gatcgactcc tgggacgtgc ttttcgatcc ggaaaaaacg gcaaagctga aagattgcgg catttacctg cttgattccg ccagcgaaat gctgcgtccg gcgctgaact atctgggtct cgacccgaac tctccgtcgc cggacgattt gcagaaggca caggatctct atctcaagat tcgtccgaat atccgcaaat tccactcgtc ggaatatatc aatgcgctcg ccaatggcga tatctgcatg gctgtcggct attccggcga tattttccag gcccgcgacc gcgccgaaaa ggcgaagcag ggggtggaga tcggctattc gatcccgaag gaaggcgcgc tgatctggtt tgaccagatg gcgatcccgg ccgatgccaa gcatgtgccg gaggcgttgg aattcatgaa ttatatgatg cgcccggagg tggcggcaaa ggcgtcgaac tatgtgttct atgccaatgg caacaaggcg tcgcagaaat tcatcgacaa ggaaatcctc gacgacccgg aaatctatcc gtccgacgag gtgatgaaga agctgttcgt gccgacgcca tatgacacga agacccagcg tgtggtcaca cgcgcctgga ccaagatcgt caccaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for BrabA.00089.a.A6.GE28053
HARVESTED ON: 12/7/2009
SEQUENCED ON: 12/23/2009
EXPECTED MW: 40kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRVVNIYNW SDYIDDSILK DFTKETGIKV VYDVYDSNEI LETKLLAGGN GYDLVVPSGE FLGRQIPAGV FLKLDKDKLP NLKNMWDEIS TRAATYDPGN EYSVNYMWGT TGIGYNKAKI KEALGTDTID SWDVLFDPEK TAKLKDCGIY LLDSASEMLR PALNYLGLDP NSPSPDDLQK AQDLYLKIRP NIRKFHSSEY INALANGDIC MAVGYSGDIF QARDRAEKAK QGVEIGYSIP KEGALIWFDQ MAIPADAKHV PEALEFMNYM MRPEVAAKAS NYVFYANGNK ASQKFIDKEI LDDPEIYPSD EVMKKLFVPT PYDTKTQRVV TRAWTKIVTG Q
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgggtggtc aatatctata actggtcgga ttatatcgac gattccatcc tcaaggactt caccaaggag accgggatca aggtcgtcta cgacgtctat gactccaacg aaattctgga aaccaagctt ctggcgggcg gcaacggcta cgacctcgtg gtgccatcgg gcgaatttct tgggcgccag attcccgcag gcgtgttcct gaaactcgac aaggacaagc tgccgaacct caagaatatg tgggatgaga tttcgacccg tgcggcaacc tatgatccgg gcaacgaata ttccgtcaat tacatgtggg gcacgaccgg catcggctac aataaggcca agatcaagga agcgctcggc accgacacga tcgactcctg ggacgtgctt ttcgatccgg aaaaaacggc aaagctgaaa gattgcggca tttacctgct tgattccgcc agcgaaatgc tgcgtccggc gctgaactat ctgggtctcg acccgaactc tccgtcgccg gacgatttgc agaaggcaca ggatctctat ctcaagattc gtccgaatat ccgcaaattc cactcgtcgg aatatatcaa tgcgctcgcc aatggcgata tctgcatggc tgtcggctat tccggcgata ttttccaggc ccgcgaccgc gccgaaaagg cgaagcaggg ggtggagatc ggctattcga tcccgaagga aggcgcgctg atctggtttg accagatggc gatcccggcc gatgccaagc atgtgccgga ggcgttggaa ttcatgaatt atatgatgcg cccggaggtg gcggcaaagg cgtcgaacta tgtgttctat gccaatggca acaaggcgtc gcagaaattc atcgacaagg aaatcctcga cgacccggaa atctatccgt ccgacgaggt gatgaagaag ctgttcgtgc cgacgccata tgacacgaag acccagcgtg tggtcacacg cgcctggacc aagatcgtca ccggccagaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for BrabA.00089.a.A7.GE28054
HARVESTED ON: 12/7/2009
SEQUENCED ON: 12/23/2009
EXPECTED MW: 40kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 97
PERCENT COVERAGE: 84
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRVVNIYNW SDYIDDSILK DFTKETGIKV VYDVYDSNEI LETKLLAGGN GYDLVVPSGE FLGRQIPAGV FLKLDKDKLP NLKNMWDEIS TRAATYDPGN EYSVNYMWGT TGIGYNKAKI KEALGTDTID SWDVLFDPEK TAKLKDCGIY LLDSASEMLR PALNYLGLDP NSPSPDDLQK AQDLYLKIRP NIRKFHSSEY INALANGDIC MAVGYSGDIF QARDRAEKAK XGVEIGYSIP KEGALIWFDQ MAIPXDXXHV PXALXFMNYM MRPEVAAKAS NXVF
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcggg tggtcaatat ctataactgg tcggattata tcgacgattc catcctcaag gacttcacca aggagaccgg gatcaaggtc gtctacgacg tctatgactc caacgaaatt ctggaaacca agcttctggc gggcggcaac ggctacgacc tcgtggtgcc atcgggcgaa tttcttgggc gccagattcc cgcaggcgtg ttcctgaaac tcgacaagga caagctgccg aacctcaaga atatgtggga tgagatttcg acccgtgcgg caacctatga tccgggcaac gaatattccg tcaattacat gtggggcacg accggcatcg gctacaataa ggccaagatc aaggaagcgc tcggcaccga cacgatcgac tcctgggacg tgcttttcga tccggaaaaa acggcaaagc tgaaagattg cggcatttac ctgcttgatt ccgccagcga aatgctgcgt ccggcgctga actatctggg tctcgacccg aactctccgt cgccggacga tttgcagaag gcacaggatc tctatctcaa gattcgtccg aatatccgca aattccactc gtcggaatat atcaatgcgc tcgccaatgg cgatatctgc atggctgtcg gctattccgg cgatattttc caggcccgcg accgcgccga aaaggcgaag canggggtgg agatcggcta ttcgatcccg aaggaaggcg cgctgatctg gtttgaccag atggcgatcc cggncgatgn cnagcatgtg ccggangcgt tggnnttcat gaattatatg atgcgcccgg aggtggcggc aaaggcgtcg aactntgtgt tct
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRVVNIYNW SDYIDDSILK DFTKETGIKV VYDVYDSNEI LETKLLAGGN GYDLVVPSGE FLGRQIPAGV FLKLDKDKLP NLKNMWDEIS TRAATYDPGN EYSVNYMWGT TGIGYNKAKI KEALGTDTID SWDVLFDPEK TAKLKDCGIY LLDSASEMLR PALNYLGLDP NSPSPDDLQK AQDLYLKIRP NIRKFHSSEY INALANGDIC MAVGYSGDIF QARDRAEKAK QGVEIGYSIP KEGALIWFDQ MAIPADAKHV PEALEFMNYM MRPEVAAKAS NYVFYANGNK ASQKFIDKEI LDDPEIYPSD EVMKKLFVPT PYDTKTQRVV TRAWTKIVT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgggtggtc aatatctata actggtcgga ttatatcgac gattccatcc tcaaggactt caccaaggag accgggatca aggtcgtcta cgacgtctat gactccaacg aaattctgga aaccaagctt ctggcgggcg gcaacggcta cgacctcgtg gtgccatcgg gcgaatttct tgggcgccag attcccgcag gcgtgttcct gaaactcgac aaggacaagc tgccgaacct caagaatatg tgggatgaga tttcgacccg tgcggcaacc tatgatccgg gcaacgaata ttccgtcaat tacatgtggg gcacgaccgg catcggctac aataaggcca agatcaagga agcgctcggc accgacacga tcgactcctg ggacgtgctt ttcgatccgg aaaaaacggc aaagctgaaa gattgcggca tttacctgct tgattccgcc agcgaaatgc tgcgtccggc gctgaactat ctgggtctcg acccgaactc tccgtcgccg gacgatttgc agaaggcaca ggatctctat ctcaagattc gtccgaatat ccgcaaattc cactcgtcgg aatatatcaa tgcgctcgcc aatggcgata tctgcatggc tgtcggctat tccggcgata ttttccaggc ccgcgaccgc gccgaaaagg cgaagcaggg ggtggagatc ggctattcga tcccgaagga aggcgcgctg atctggtttg accagatggc gatcccggcc gatgccaagc atgtgccgga ggcgttggaa ttcatgaatt atatgatgcg cccggaggtg gcggcaaagg cgtcgaacta tgtgttctat gccaatggca acaaggcgtc gcagaaattc atcgacaagg aaatcctcga cgacccggaa atctatccgt ccgacgaggt gatgaagaag ctgttcgtgc cgacgccata tgacacgaag acccagcgtg tggtcacacg cgcctggacc aagatcgtca ccaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for BrabA.00089.a.A4.PS00950
PURIFICATION DATe: 1/25/2011
CONCENTRATION: 23.56mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 12
VIAL VOLUME: 200µl
PERCENT IDENTITY: 98
PERCENT COVERAGE: 92
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGAQERVVN IYNWSDYIDD SILKDFTKET GIKVVYDVYD SNEILETKLL AGGNGYDLVV PSGEFLGRQI PAGVFLKLDK DKLPNLKNMW DEISTRAATY DPGNEYSVNY MWGTTGIGYN KAKIKEALGT DTIDSWDVLF DPEKTAKLKD CGIYLLDSAS EMLRPALNYL GLDPNSPSPD DLQKAQDLYL KIRPNIRKFH SSEYINALAN GDICMAVGYS GDIFQARDRA EKAKQGVEIG YSIPKEGALI WFDQMAIPAD AKHVPEALEF MNYMMRPEVA AKASNYVFYA NGNKASXKFI DKEIXDDPEI YPSXXX
Validated NT Sequence
aattttgntt nnctttaaga aggagatata ccatggctca tcaccatcac catcatatgg gtaccctgga agctcagacc cagggtcctg gttcgatggg cgcgcaggag cgggtggtca atatctataa ctggtcggat tatatcgacg attccatcct caaggacttc accaaggaga ccgggatcaa ggtcgtctac gacgtctatg actccaacga aattctggaa accaagcttc tggcgggcgg caacggctac gacctcgtgg tgccatcggg cgaatttctt gggcgccaga ttcccgcagg cgtgttcctg aaactcgaca aggacaagct gccgaacctc aagaatatgt gggatgagat ttcgacccgt gcggcaacct atgatccggg caacgaatat tccgtcaatt acatgtgggg cacgaccggc atcggctaca ataaggccaa gatcaaggaa gcgctcggca ccgacacgat cgactcctgg gacgtgcttt tcgatccgga aaaaacggca aagctgaaag attgcggcat ttacctgctt gattccgcca gcgaaatgct gcgtccggcg ctgaactatc tgggtctcga cccgaactct ccgtcgccgg acgatttgca gaaggcacag gatctctatc tcaagattcg tccgaatatc cgcaaattcc actcgtcgga atatatcaat gcgctcgcca atggcgatat ctgcatggct gtcggctatt ccggcgatat tttccaggcc cgcgaccgcg ccgaaaaggc gaagcagggg gtggagatcg gctattcgat cccgaaggaa ggcgcgctga tctggtttga ccagatggcg atcccggccg atgccaagca tgtgccggag gcgttggaat tcatgaatta tatgatgcgc ccggaggtgg cggcaaaggc gtcnaactat gtgttctatg ccaatggcaa caaggcgtcg canaaattca tcgacaagga aatccncgac gacccngaaa tctatccgtc cgannannna tgananctnn tcntgccnnc ncntatgann cnanacccnn gnnnncncnn ncnncnggnc nannatcgnc nnnnnntann naacnannna ntnntgcann nnntnnnnnn ngnnnnnnnn nancnnannt nnnnnnnnnn ncncnngnnn nnnngnnnnn nnnnnnnngg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGAQERVVN IYNWSDYIDD SILKDFTKET GIKVVYDVYD SNEILETKLL AGGNGYDLVV PSGEFLGRQI PAGVFLKLDK DKLPNLKNMW DEISTRAATY DPGNEYSVNY MWGTTGIGYN KAKIKEALGT DTIDSWDVLF DPEKTAKLKD CGIYLLDSAS EMLRPALNYL GLDPNSPSPD DLQKAQDLYL KIRPNIRKFH SSEYINALAN GDICMAVGYS GDIFQARDRA EKAKQGVEIG YSIPKEGALI WFDQMAIPAD AKHVPEALEF MNYMMRPEVA AKASNYVFYA NGNKASQKFI DKEILDDPEI YPSDEVMKKL FVPTPYDTKT QRVVTRAWTK IVTGQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGCGCGCAG GAGCGGGTGG TCAATATCTA TAACTGGTCG GATTATATCG ACGATTCCAT CCTCAAGGAC TTCACCAAGG AGACCGGGAT CAAGGTCGTC TACGACGTCT ATGACTCCAA CGAAATTCTG GAAACCAAGC TTCTGGCGGG CGGCAACGGC TACGACCTCG TGGTGCCATC GGGCGAATTT CTTGGGCGCC AGATTCCCGC AGGCGTGTTC CTGAAACTCG ACAAGGACAA GCTGCCGAAC CTCAAGAATA TGTGGGATGA GATTTCGACC CGTGCGGCAA CCTATGATCC GGGCAACGAA TATTCCGTCA ATTACATGTG GGGCACGACC GGCATCGGCT ACAATAAGGC CAAGATCAAG GAAGCGCTCG GCACCGACAC GATCGACTCC TGGGACGTGC TTTTCGATCC GGAAAAAACG GCAAAGCTGA AAGATTGCGG CATTTACCTG CTTGATTCCG CCAGCGAAAT GCTGCGTCCG GCGCTGAACT ATCTGGGTCT CGACCCGAAC TCTCCGTCGC CGGACGATTT GCAGAAGGCA CAGGATCTCT ATCTCAAGAT TCGTCCGAAT ATCCGCAAAT TCCACTCGTC GGAATATATC AATGCGCTCG CCAATGGCGA TATCTGCATG GCTGTCGGCT ATTCCGGCGA TATTTTCCAG GCCCGCGACC GCGCCGAAAA GGCGAAGCAG GGGGTGGAGA TCGGCTATTC GATCCCGAAG GAAGGCGCGC TGATCTGGTT TGACCAGATG GCGATCCCGG CCGATGCCAA GCATGTGCCG GAGGCGTTGG AATTCATGAA TTATATGATG CGCCCGGAGG TGGCGGCAAA GGCGTCGAAC TATGTGTTCT ATGCCAATGG CAACAAGGCG TCGCAGAAAT TCATCGACAA GGAAATCCTC GACGACCCGG AAATCTATCC GTCCGACGAG GTGATGAAGA AGCTGTTCGT GCCGACGCCA TATGACACGA AGACCCAGCG TGTGGTCACA CGCGCCTGGA CCAAGATCGT CACCGGCCAG AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA
Details for BrabA.00089.a.A3.PW27943
PURIFICATION DATe: 2/22/2010
CONCENTRATION: 24.09mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 100µl
PERCENT IDENTITY: 98
PERCENT COVERAGE: 68
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMGIKSFL LATTVATGFV AAATFSAGAQ ERVVNIYNWS DYIDDSILKD FTKETGIKVV YDVYDSNEIL ETKLLAGGNG YDLVVPSGEF LGRQIPAGVF LKLDKDKLPN LKNMWDEIST RAATYDPGNE YSVNYMWGTT GIGYNKAKIK EALGTDTIDS WDVLFDPEKT AKLKDCGIYL LDSASEMLRP ALNYLGLDPN SPSPXDLQXX QDLYLKIRPN IRKFHSSEYI NALAXGDXCM AVGYSGDIFQ ARDR
Validated NT Sequence
tcaccatcat atgggtaccc tggaagctca gacccagggt cctggttcga tggggatcaa atccttcctt ctggcaacga ccgttgcgac gggttttgtc gcggctgcca cattttccgc aggcgcgcag gagcgggtgg tcaatatcta taactggtcg gattatatcg acgattccat cctcaaggac ttcaccaagg agaccgggat caaggtcgtc tacgacgtct atgactccaa cgaaattctg gaaaccaagc ttctggcggg cggcaacggc tacgacctcg tggtgccatc gggcgaattt cttgggcgcc agattcccgc aggcgtgttc ctgaaactcg acaaggacaa gctgccgaac ctcaagaata tgtgggatga gatttcgacc cgtgcggcaa cctatgatcc gggcaacgaa tattccgtca attacatgtg gggcacgacc ggcatcggct acaataaggc caagatcaag gaagcgctcg gcaccgacac gatcgactcc tgggacgtgc ttttcgatcc ggaaaaaacg gcaaagctga aagattgcgg catttacctg cttgattccg ccagcgaaat gctgcgtccg gcgctgaact atctgggtct cgacccgaac tctccgtcgc cggangattt gcagaangna caggatctct atctcaagat tcgtccgaat atccgcaaat tccactcgtc ngaatatatc aatgcgctcg ccnatggcga tntctgcatg gctgtcggct attccggcga tattttccag gcccgcgacc gcgcc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGIKSFLLA TTVATGFVAA ATFSAGAQER VVNIYNWSDY IDDSILKDFT KETGIKVVYD VYDSNEILET KLLAGGNGYD LVVPSGEFLG RQIPAGVFLK LDKDKLPNLK NMWDEISTRA ATYDPGNEYS VNYMWGTTGI GYNKAKIKEA LGTDTIDSWD VLFDPEKTAK LKDCGIYLLD SASEMLRPAL NYLGLDPNSP SPDDLQKAQD LYLKIRPNIR KFHSSEYINA LANGDICMAV GYSGDIFQAR DRAEKAKQGV EIGYSIPKEG ALIWFDQMAI PADAKHVPEA LEFMNYMMRP EVAAKASNYV FYANGNKASQ KFIDKEILDD PEIYPSDEVM KKLFVPTPYD TKTQRVVTRA WTKIVT
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGGATCAAA TCCTTCCTTC TGGCAACGAC CGTTGCGACG GGTTTTGTCG CGGCTGCCAC ATTTTCCGCA GGCGCGCAGG AGCGGGTGGT CAATATCTAT AACTGGTCGG ATTATATCGA CGATTCCATC CTCAAGGACT TCACCAAGGA GACCGGGATC AAGGTCGTCT ACGACGTCTA TGACTCCAAC GAAATTCTGG AAACCAAGCT TCTGGCGGGC GGCAACGGCT ACGACCTCGT GGTGCCATCG GGCGAATTTC TTGGGCGCCA GATTCCCGCA GGCGTGTTCC TGAAACTCGA CAAGGACAAG CTGCCGAACC TCAAGAATAT GTGGGATGAG ATTTCGACCC GTGCGGCAAC CTATGATCCG GGCAACGAAT ATTCCGTCAA TTACATGTGG GGCACGACCG GCATCGGCTA CAATAAGGCC AAGATCAAGG AAGCGCTCGG CACCGACACG ATCGACTCCT GGGACGTGCT TTTCGATCCG GAAAAAACGG CAAAGCTGAA AGATTGCGGC ATTTACCTGC TTGATTCCGC CAGCGAAATG CTGCGTCCGG CGCTGAACTA TCTGGGTCTC GACCCGAACT CTCCGTCGCC GGACGATTTG CAGAAGGCAC AGGATCTCTA TCTCAAGATT CGTCCGAATA TCCGCAAATT CCACTCGTCG GAATATATCA ATGCGCTCGC CAATGGCGAT ATCTGCATGG CTGTCGGCTA TTCCGGCGAT ATTTTCCAGG CCCGCGACCG CGCCGAAAAG GCGAAGCAGG GGGTGGAGAT CGGCTATTCG ATCCCGAAGG AAGGCGCGCT GATCTGGTTT GACCAGATGG CGATCCCGGC CGATGCCAAG CATGTGCCGG AGGCGTTGGA ATTCATGAAT TATATGATGC GCCCGGAGGT GGCGGCAAAG GCGTCGAACT ATGTGTTCTA TGCCAATGGC AACAAGGCGT CGCAGAAATT CATCGACAAG GAAATCCTCG ACGACCCGGA AATCTATCCG TCCGACGAGG TGATGAAGAA GCTGTTCGTG CCGACGCCAT ATGACACGAA GACCCAGCGT GTGGTCACAC GCGCCTGGAC CAAGATCGTC ACCAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA
Details for BrabA.00089.a.A5.PW27945
PURIFICATION DATe: 2/22/2010
CONCENTRATION: 27.47mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 22
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 85
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGAQERVVN IYNWSDYIDD SILKDFTKET GIKVVYDVYD SNEILETKLL AGGNGYDLVV PSGEFLGRQI PAGVFLKLDK DKLPNLKNMW DEISTRAATY DPGNEYSVNY MWGTTGIGYN KAKIKEALGT DTIDSWDVLF DPEKTAKLKD CGIYLLDSAS EMLRPALNYL GLDPNSPSPD DLQKAQDLYL KIRPNIRKFH SSEYINALAN GDICMAVGYS GDIFQARDRA EKAKQGVEIG YSIPKEGALI WFDQMAIPAD AKHVPXALEF MNYMMRPEVA AKASNYVFY
Validated NT Sequence
tttaagaagg aganntacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgggcgc gcaggagcgg gtggtcaata tctataactg gtcggattat atcgacgatt ccatcctcaa ggacttcacc aaggagaccg ggatcaaggt cgtctacgac gtctatgact ccaacgaaat tctggaaacc aagcttctgg cgggcggcaa cggctacgac ctcgtggtgc catcgggcga atttcttggg cgccagattc ccgcaggcgt gttcctgaaa ctcgacaagg acaagctgcc gaacctcaag aatatgtggg atgagatttc gacccgtgcg gcaacctatg atccgggcaa cgaatattcc gtcaattaca tgtggggcac gaccggcatc ggctacaata aggccaagat caaggaagcg ctcggcaccg acacgatcga ctcctgggac gtgcttttcg atccggaaaa aacggcaaag ctgaaagatt gcggcattta cctgcttgat tccgccagcg aaatgctgcg tccggcgctg aactatctgg gtctcgaccc gaactctccg tcgccggacg atttgcagaa ggcacaggat ctctatctca agattcgtcc gaatatccgc aaattccact cgtcggaata tatcaatgcg ctcgccaatg gcgatatctg catggctgtc ggctattccg gcgatatttt ccaggcccgc gaccgcgccg aaaaggcgaa gcagggggtg gagatcggct attcgatccc gaaggaaggc gcgctgatct ggtttgacca gatggcgatc ccggccgatg ccaagcatgt gccggangcg ttggaattca tgaattatat gatgcgcccg gaggtggcgg caaaggcgtc gaactatgtg ttctatgnc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGAQERVVN IYNWSDYIDD SILKDFTKET GIKVVYDVYD SNEILETKLL AGGNGYDLVV PSGEFLGRQI PAGVFLKLDK DKLPNLKNMW DEISTRAATY DPGNEYSVNY MWGTTGIGYN KAKIKEALGT DTIDSWDVLF DPEKTAKLKD CGIYLLDSAS EMLRPALNYL GLDPNSPSPD DLQKAQDLYL KIRPNIRKFH SSEYINALAN GDICMAVGYS GDIFQARDRA EKAKQGVEIG YSIPKEGALI WFDQMAIPAD AKHVPEALEF MNYMMRPEVA AKASNYVFYA NGNKASQKFI DKEILDDPEI YPSDEVMKKL FVPTPYDTKT QRVVTRAWTK IVT
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGCGCGCAG GAGCGGGTGG TCAATATCTA TAACTGGTCG GATTATATCG ACGATTCCAT CCTCAAGGAC TTCACCAAGG AGACCGGGAT CAAGGTCGTC TACGACGTCT ATGACTCCAA CGAAATTCTG GAAACCAAGC TTCTGGCGGG CGGCAACGGC TACGACCTCG TGGTGCCATC GGGCGAATTT CTTGGGCGCC AGATTCCCGC AGGCGTGTTC CTGAAACTCG ACAAGGACAA GCTGCCGAAC CTCAAGAATA TGTGGGATGA GATTTCGACC CGTGCGGCAA CCTATGATCC GGGCAACGAA TATTCCGTCA ATTACATGTG GGGCACGACC GGCATCGGCT ACAATAAGGC CAAGATCAAG GAAGCGCTCG GCACCGACAC GATCGACTCC TGGGACGTGC TTTTCGATCC GGAAAAAACG GCAAAGCTGA AAGATTGCGG CATTTACCTG CTTGATTCCG CCAGCGAAAT GCTGCGTCCG GCGCTGAACT ATCTGGGTCT CGACCCGAAC TCTCCGTCGC CGGACGATTT GCAGAAGGCA CAGGATCTCT ATCTCAAGAT TCGTCCGAAT ATCCGCAAAT TCCACTCGTC GGAATATATC AATGCGCTCG CCAATGGCGA TATCTGCATG GCTGTCGGCT ATTCCGGCGA TATTTTCCAG GCCCGCGACC GCGCCGAAAA GGCGAAGCAG GGGGTGGAGA TCGGCTATTC GATCCCGAAG GAAGGCGCGC TGATCTGGTT TGACCAGATG GCGATCCCGG CCGATGCCAA GCATGTGCCG GAGGCGTTGG AATTCATGAA TTATATGATG CGCCCGGAGG TGGCGGCAAA GGCGTCGAAC TATGTGTTCT ATGCCAATGG CAACAAGGCG TCGCAGAAAT TCATCGACAA GGAAATCCTC GACGACCCGG AAATCTATCC GTCCGACGAG GTGATGAAGA AGCTGTTCGT GCCGACGCCA TATGACACGA AGACCCAGCG TGTGGTCACA CGCGCCTGGA CCAAGATCGT CACCAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA
Details for BrabA.00089.a.A6.PW27946
PURIFICATION DATe: 2/22/2010
CONCENTRATION: 20.39mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 27
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRVVNIYNW SDYIDDSILK DFTKETGIKV VYDVYDSNEI LETKLLAGGN GYDLVVPSGE FLGRQIPAGV FLKLDKDKLP NLKNMWDEIS TRAATYDPGN EYSVNYMWGT TGIGYNKAKI KEALGTDTID SWDVLFDPEK TAKLKDCGIY LLDSASEMLR PALNYLGLDP NSPSPDDLQK AQDLYLKIRP NIRKFHSSEY INALANGDIC MAVGYSGDIF QARDRAEKAK QGVEIGYSIP KEGALIWFDQ MAIPADAKHV PEALEFMNYM MRPEVAAKAS NYVFYANGNK ASQKFIDKEI LDDPEIYPSD EVMKKLFVPT PYDTKTQRVV TRAWTKIVTG Q
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCGGGTGGTC AATATCTATA ACTGGTCGGA TTATATCGAC GATTCCATCC TCAAGGACTT CACCAAGGAG ACCGGGATCA AGGTCGTCTA CGACGTCTAT GACTCCAACG AAATTCTGGA AACCAAGCTT CTGGCGGGCG GCAACGGCTA CGACCTCGTG GTGCCATCGG GCGAATTTCT TGGGCGCCAG ATTCCCGCAG GCGTGTTCCT GAAACTCGAC AAGGACAAGC TGCCGAACCT CAAGAATATG TGGGATGAGA TTTCGACCCG TGCGGCAACC TATGATCCGG GCAACGAATA TTCCGTCAAT TACATGTGGG GCACGACCGG CATCGGCTAC AATAAGGCCA AGATCAAGGA AGCGCTCGGC ACCGACACGA TCGACTCCTG GGACGTGCTT TTCGATCCGG AAAAAACGGC AAAGCTGAAA GATTGCGGCA TTTACCTGCT TGATTCCGCC AGCGAAATGC TGCGTCCGGC GCTGAACTAT CTGGGTCTCG ACCCGAACTC TCCGTCGCCG GACGATTTGC AGAAGGCACA GGATCTCTAT CTCAAGATTC GTCCGAATAT CCGCAAATTC CACTCGTCGG AATATATCAA TGCGCTCGCC AATGGCGATA TCTGCATGGC TGTCGGCTAT TCCGGCGATA TTTTCCAGGC CCGCGACCGC GCCGAAAAGG CGAAGCAGGG GGTGGAGATC GGCTATTCGA TCCCGAAGGA AGGCGCGCTG ATCTGGTTTG ACCAGATGGC GATCCCGGCC GATGCCAAGC ATGTGCCGGA GGCGTTGGAA TTCATGAATT ATATGATGCG CCCGGAGGTG GCGGCAAAGG CGTCGAACTA TGTGTTCTAT GCCAATGGCA ACAAGGCGTC GCAGAAATTC ATCGACAAGG AAATCCTCGA CGACCCGGAA ATCTATCCGT CCGACGAGGT GATGAAGAAG CTGTTCGTGC CGACGCCATA TGACACGAAG ACCCAGCGTG TGGTCACACG CGCCTGGACC AAGATCGTCA CCGGCCAGAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA
Details for BrabA.00089.a.A7.PW27947
PURIFICATION DATe: 2/22/2010
CONCENTRATION: 27.87mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 13
VIAL VOLUME: 200µl
PERCENT IDENTITY: 97
PERCENT COVERAGE: 84
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRVVNIYNW SDYIDDSILK DFTKETGIKV VYDVYDSNEI LETKLLAGGN GYDLVVPSGE FLGRQIPAGV FLKLDKDKLP NLKNMWDEIS TRAATYDPGN EYSVNYMWGT TGIGYNKAKI KEALGTDTID SWDVLFDPEK TAKLKDCGIY LLDSASEMLR PALNYLGLDP NSPSPDDLQK AQDLYLKIRP NIRKFHSSEY INALANGDIC MAVGYSGDIF QARDRAEKAK XGVEIGYSIP KEGALIWFDQ MAIPXDXXHV PXALXFMNYM MRPEVAAKAS NXVF
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcggg tggtcaatat ctataactgg tcggattata tcgacgattc catcctcaag gacttcacca aggagaccgg gatcaaggtc gtctacgacg tctatgactc caacgaaatt ctggaaacca agcttctggc gggcggcaac ggctacgacc tcgtggtgcc atcgggcgaa tttcttgggc gccagattcc cgcaggcgtg ttcctgaaac tcgacaagga caagctgccg aacctcaaga atatgtggga tgagatttcg acccgtgcgg caacctatga tccgggcaac gaatattccg tcaattacat gtggggcacg accggcatcg gctacaataa ggccaagatc aaggaagcgc tcggcaccga cacgatcgac tcctgggacg tgcttttcga tccggaaaaa acggcaaagc tgaaagattg cggcatttac ctgcttgatt ccgccagcga aatgctgcgt ccggcgctga actatctggg tctcgacccg aactctccgt cgccggacga tttgcagaag gcacaggatc tctatctcaa gattcgtccg aatatccgca aattccactc gtcggaatat atcaatgcgc tcgccaatgg cgatatctgc atggctgtcg gctattccgg cgatattttc caggcccgcg accgcgccga aaaggcgaag canggggtgg agatcggcta ttcgatcccg aaggaaggcg cgctgatctg gtttgaccag atggcgatcc cggncgatgn cnagcatgtg ccggangcgt tggnnttcat gaattatatg atgcgcccgg aggtggcggc aaaggcgtcg aactntgtgt tct
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRVVNIYNW SDYIDDSILK DFTKETGIKV VYDVYDSNEI LETKLLAGGN GYDLVVPSGE FLGRQIPAGV FLKLDKDKLP NLKNMWDEIS TRAATYDPGN EYSVNYMWGT TGIGYNKAKI KEALGTDTID SWDVLFDPEK TAKLKDCGIY LLDSASEMLR PALNYLGLDP NSPSPDDLQK AQDLYLKIRP NIRKFHSSEY INALANGDIC MAVGYSGDIF QARDRAEKAK QGVEIGYSIP KEGALIWFDQ MAIPADAKHV PEALEFMNYM MRPEVAAKAS NYVFYANGNK ASQKFIDKEI LDDPEIYPSD EVMKKLFVPT PYDTKTQRVV TRAWTKIVT
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCGGGTGGTC AATATCTATA ACTGGTCGGA TTATATCGAC GATTCCATCC TCAAGGACTT CACCAAGGAG ACCGGGATCA AGGTCGTCTA CGACGTCTAT GACTCCAACG AAATTCTGGA AACCAAGCTT CTGGCGGGCG GCAACGGCTA CGACCTCGTG GTGCCATCGG GCGAATTTCT TGGGCGCCAG ATTCCCGCAG GCGTGTTCCT GAAACTCGAC AAGGACAAGC TGCCGAACCT CAAGAATATG TGGGATGAGA TTTCGACCCG TGCGGCAACC TATGATCCGG GCAACGAATA TTCCGTCAAT TACATGTGGG GCACGACCGG CATCGGCTAC AATAAGGCCA AGATCAAGGA AGCGCTCGGC ACCGACACGA TCGACTCCTG GGACGTGCTT TTCGATCCGG AAAAAACGGC AAAGCTGAAA GATTGCGGCA TTTACCTGCT TGATTCCGCC AGCGAAATGC TGCGTCCGGC GCTGAACTAT CTGGGTCTCG ACCCGAACTC TCCGTCGCCG GACGATTTGC AGAAGGCACA GGATCTCTAT CTCAAGATTC GTCCGAATAT CCGCAAATTC CACTCGTCGG AATATATCAA TGCGCTCGCC AATGGCGATA TCTGCATGGC TGTCGGCTAT TCCGGCGATA TTTTCCAGGC CCGCGACCGC GCCGAAAAGG CGAAGCAGGG GGTGGAGATC GGCTATTCGA TCCCGAAGGA AGGCGCGCTG ATCTGGTTTG ACCAGATGGC GATCCCGGCC GATGCCAAGC ATGTGCCGGA GGCGTTGGAA TTCATGAATT ATATGATGCG CCCGGAGGTG GCGGCAAAGG CGTCGAACTA TGTGTTCTAT GCCAATGGCA ACAAGGCGTC GCAGAAATTC ATCGACAAGG AAATCCTCGA CGACCCGGAA ATCTATCCGT CCGACGAGGT GATGAAGAAG CTGTTCGTGC CGACGCCATA TGACACGAAG ACCCAGCGTG TGGTCACACG CGCCTGGACC AAGATCGTCA CCAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA