BrabA.00085.b

NAD binding site:NAD-dependent epimerase/dehydratase:UDP-glucose 4-epimerase

CENTER ID:	BrabA.00085.b
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.00085.b.A1.GE25394	Full length( BrabA.00085.b )		1	335

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.00085.b.A1.PS00054	Full length( BrabA.00085.b )		1	335

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_126853
PATRIC ID:	fig\|359391.4.peg.1133
RefSeq:	YP_414484
UniProt:	Q2YQ12

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTILVTGGAG YIGSHTCVQL IEAGHEVVVV DNFDNSHPEA LHRIEKITGR APRREPGDIR DRALMEQVIK RHKCTAVIHF AGLKAVGESS EKPLLYYDCN VLGTLRLLQA MEATGVKKLV FSSSATVYGD PDKLPITEDQ PLSATNPYGR TKLVIEDMLR DLYNSDNSWA IAILRYFNPV GAHESGLIGE DPKGIPNNLM PIIAQVATGR REKLNIWGND YPTPDGTGVR DYIHVNDLAA GHLKALKKLD KPKCFAVNLG TGQGYSVLDV IKAFEHVSNR EIKYEIAPRR PGDVAECYAD PGFAKKFLGW SAEKNLREMC QDMWNWQSKN PNGYE

NT Sequence

ATGACAATTC TTGTAACAGG TGGTGCTGGC TATATCGGCT CCCACACGTG TGTGCAGTTG ATCGAGGCAG GCCATGAAGT GGTTGTGGTC GATAATTTCG ACAACAGCCA TCCTGAGGCA CTGCATCGGA TTGAAAAGAT CACGGGCCGC GCGCCGCGCC GCGAACCGGG CGATATTCGC GATCGCGCCC TTATGGAACA GGTGATCAAA CGCCATAAAT GCACTGCGGT TATCCATTTT GCCGGGCTGA AGGCCGTGGG TGAATCGAGC GAAAAGCCGC TGCTCTATTA TGATTGCAAT GTGCTGGGCA CACTTCGGCT TCTGCAGGCC ATGGAAGCGA CAGGCGTGAA GAAGCTCGTT TTCAGCTCTT CGGCCACCGT CTATGGCGAC CCGGATAAAC TGCCGATCAC CGAAGATCAG CCCCTTTCGG CCACCAATCC CTATGGCCGG ACCAAGCTTG TCATCGAAGA CATGCTGCGC GACCTTTATA ACAGTGACAA TAGCTGGGCG ATTGCGATTC TGCGCTATTT CAATCCTGTC GGCGCTCATG AAAGCGGGCT TATCGGTGAA GACCCGAAGG GTATTCCCAA CAATCTGATG CCCATTATTG CTCAGGTCGC AACTGGACGA CGCGAAAAGC TGAACATCTG GGGCAACGAC TATCCGACAC CGGATGGCAC CGGCGTACGC GACTATATCC ATGTCAACGA TCTGGCTGCC GGGCACCTCA AGGCCCTGAA AAAGCTGGAT AAGCCCAAGT GCTTCGCCGT CAATCTTGGA ACGGGGCAGG GCTATAGTGT TCTTGATGTG ATCAAGGCGT TTGAACATGT CTCCAATCGC GAGATCAAAT ATGAGATTGC GCCGCGCCGT CCCGGCGATG TTGCCGAATG CTATGCCGAT CCCGGCTTTG CAAAGAAATT TCTGGGCTGG TCGGCTGAGA AAAACCTGCG TGAAATGTGT CAGGACATGT GGAACTGGCA ATCGAAAAAT CCGAACGGCT ACGAA

Details for BrabA.00085.b.A1.GE25394

HARVESTED ON:	6/30/2008
SEQUENCED ON:	6/16/2009
EXPECTED MW:	39kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	94
PERCENT COVERAGE:	48

Validated AA Sequence

MTILVTGGAG YIGSHTCVQL IEAGHEVVVV DNFDNSHPEA LHRIEKITGR APRREPGDIR DRALMEQVIK RHKCTAVIHF AGLKAVGESS EKPLLYYDCN VLGTLRLLQA MEATGVKKLV FSSSATVYGD PDKLPITEDQ PLSATNPYGR TKLVXXDXXX XXYXXDXSWA IAI

Validated NT Sequence

ccctggaagc tcagacccag ggtcctggtt cgatgacaat tcttgtaaca ggtggtgctg gctatatcgg ctcccacacg tgtgtgcagt tgatcgaggc aggccatgaa gtggttgtgg tcgataattt cgacaacagc catcctgagg cactgcatcg gattgaaaag atcacgggcc gcgcgccgcg ccgcgaaccg ggcgatattc gcgatcgcgc ccttatggaa caggtgatca aacgccataa atgcactgcg gttatccatt ttgccgggct gaaggccgtg ggtgaatcga gcgaaaagcc gctgctctat tatgattgca atgtgctggg cacacttcgg cttctgcagg ccatggaagc gacaggcgtg aagaagctcg ttttcagctc ttcggccacc gtctatggcg acccggataa actgccgatc accgaagatc agcccctttc ggccaccaat ccctatggnc ggaccaagct tgtnnncnaa gacnngntgc ncgnnnnnta tnnnanngac aanagctggg cgattgcgat tctg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTILVTGGA GYIGSHTCVQ LIEAGHEVVV VDNFDNSHPE ALHRIEKITG RAPRREPGDI RDRALMEQVI KRHKCTAVIH FAGLKAVGES SEKPLLYYDC NVLGTLRLLQ AMEATGVKKL VFSSSATVYG DPDKLPITED QPLSATNPYG RTKLVIEDML RDLYNSDNSW AIAILRYFNP VGAHESGLIG EDPKGIPNNL MPIIAQVATG RREKLNIWGN DYPTPDGTGV RDYIHVNDLA AGHLKALKKL DKPKCFAVNL GTGQGYSVLD VIKAFEHVSN REIKYEIAPR RPGDVAECYA DPGFAKKFLG WSAEKNLREM CQDMWNWQSK NPNGYE

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacaattctt gtaacaggtg gtgctggcta tatcggctcc cacacgtgtg tgcagttgat cgaggcaggc catgaagtgg ttgtggtcga taatttcgac aacagccatc ctgaggcact gcatcggatt gaaaagatca cgggccgcgc gccgcgccgc gaaccgggcg atattcgcga tcgcgccctt atggaacagg tgatcaaacg ccataaatgc actgcggtta tccattttgc cgggctgaag gccgtgggtg aatcgagcga aaagccgctg ctctattatg attgcaatgt gctgggcaca cttcggcttc tgcaggccat ggaagcgaca ggcgtgaaga agctcgtttt cagctcttcg gccaccgtct atggcgaccc ggataaactg ccgatcaccg aagatcagcc cctttcggcc accaatccct atggccggac caagcttgtc atcgaagaca tgctgcgcga cctttataac agtgacaata gctgggcgat tgcgattctg cgctatttca atcctgtcgg cgctcatgaa agcgggctta tcggtgaaga cccgaagggt attcccaaca atctgatgcc cattattgct caggtcgcaa ctggacgacg cgaaaagctg aacatctggg gcaacgacta tccgacaccg gatggcaccg gcgtacgcga ctatatccat gtcaacgatc tggctgccgg gcacctcaag gccctgaaaa agctggataa gcccaagtgc ttcgccgtca atcttggaac ggggcagggc tatagtgttc ttgatgtgat caaggcgttt gaacatgtct ccaatcgcga gatcaaatat gagattgcgc cgcgccgtcc cggcgatgtt gccgaatgct atgccgatcc cggctttgca aagaaatttc tgggctggtc ggctgagaaa aacctgcgtg aaatgtgtca ggacatgtgg aactggcaat cgaaaaatcc gaacggctac gaataaaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa

Details for BrabA.00085.b.A1.PS00054

PURIFICATION DATe:	8/13/2008
CONCENTRATION:	37.8mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	18
VIAL VOLUME:	100µl
PERCENT IDENTITY:	94
PERCENT COVERAGE:	48

Protocol Notes

notes unavailable

Validated AA Sequence

MTILVTGGAG YIGSHTCVQL IEAGHEVVVV DNFDNSHPEA LHRIEKITGR APRREPGDIR DRALMEQVIK RHKCTAVIHF AGLKAVGESS EKPLLYYDCN VLGTLRLLQA MEATGVKKLV FSSSATVYGD PDKLPITEDQ PLSATNPYGR TKLVXXDXXX XXYXXDXSWA IAI

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACAATTCTT GTAACAGGTG GTGCTGGCTA TATCGGCTCC CACACGTGTG TGCAGTTGAT CGAGGCAGGC CATGAAGTGG TTGTGGTCGA TAATTTCGAC AACAGCCATC CTGAGGCACT GCATCGGATT GAAAAGATCA CGGGCCGCGC GCCGCGCCGC GAACCGGGCG ATATTCGCGA TCGCGCCCTT ATGGAACAGG TGATCAAACG CCATAAATGC ACTGCGGTTA TCCATTTTGC CGGGCTGAAG GCCGTGGGTG AATCGAGCGA AAAGCCGCTG CTCTATTATG ATTGCAATGT GCTGGGCACA CTTCGGCTTC TGCAGGCCAT GGAAGCGACA GGCGTGAAGA AGCTCGTTTT CAGCTCTTCG GCCACCGTCT ATGGCGACCC GGATAAACTG CCGATCACCG AAGATCAGCC CCTTTCGGCC ACCAATCCCT ATGGCCGGAC CAAGCTTGTC ATCGAAGACA TGCTGCGCGA CCTTTATAAC AGTGACAATA GCTGGGCGAT TGCGATTCTG CGCTATTTCA ATCCTGTCGG CGCTCATGAA AGCGGGCTTA TCGGTGAAGA CCCGAAGGGT ATTCCCAACA ATCTGATGCC CATTATTGCT CAGGTCGCAA CTGGACGACG CGAAAAGCTG AACATCTGGG GCAACGACTA TCCGACACCG GATGGCACCG GCGTACGCGA CTATATCCAT GTCAACGATC TGGCTGCCGG GCACCTCAAG GCCCTGAAAA AGCTGGATAA GCCCAAGTGC TTCGCCGTCA ATCTTGGAAC GGGGCAGGGC TATAGTGTTC TTGATGTGAT CAAGGCGTTT GAACATGTCT CCAATCGCGA GATCAAATAT GAGATTGCGC CGCGCCGTCC CGGCGATGTT GCCGAATGCT ATGCCGATCC CGGCTTTGCA AAGAAATTTC TGGGCTGGTC GGCTGAGAAA AACCTGCGTG AAATGTGTCA GGACATGTGG AACTGGCAAT CGAAAAATCC GAACGGCTAC GAATAAAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA