BrabA.00085.a
Short-chain dehydrogenase/reductase SDR:GDP-mannose 4,6-dehydratase
| CENTER ID: | BrabA.00085.a |
| ORGANISM: | Brucella melitensis biovar Abortus 2308 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.00085.a.A1.GE25393 | Full length( BrabA.00085.a ) | 1 | 362 | ||
| BrabA.00085.a.AE1.GE43128 | Full length( BrabA.00085.a ) | 1 | 362 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.00085.a.A1.PS00056 | Full length( BrabA.00085.a ) | 1 | 362 | ||
| BrabA.00085.a.A1.PS00349 | Full length( BrabA.00085.a ) | 1 | 362 | ||
| BrabA.00085.a.AE1.PW38674 | Full length( BrabA.00085.a ) | 1 | 362 |
Structures
7KF3
DEPOSITED: 10/13/2020
DETERMINATION: XRay
CLONE: BrabA.00085.a.AE1.GE43128
PROTEIN: BrabA.00085.a.AE1.PW38674
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_128711 |
| PATRIC ID: | fig|359391.4.peg.572 |
| RefSeq: | YP_413999 |
| UniProt: | Q2YMP3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKVALITGI TGQDGAYLAE LLLQKGYAVH GIKRRTSQFN TARIDHLYHD PHEQGVDLTL HHGDLTDTSS LVRIMQLVRP DEVYNLGAQS HVAVSFEEPE YTANSDALGA LRLLEAIRIL GMEKQTRYYQ ASTSELYGLV QEIPQKETTP FYPRSPYAAA KLYAYWITVN YREAYGIYAC NGILFNHESP LRGETFVTRK ITRALARIKI GNQNRLYLGN LDSLRDWGHA RDYVEMQWLM LQQDQPEDFV IATGKQYSVR EFVTLAAKDI GIEMRWEGSG EAEKGYDAKT GACIVEVDPR YFRPAEVETL LGDATKAHQK LGWKPKISFE TLVSEMIAED LAAAARENLL REHGHQFFAP KE
NT Sequence
GTGAAGAAAG TCGCACTTAT CACAGGTATC ACCGGCCAAG ACGGCGCCTA CCTTGCGGAA TTGCTTCTAC AAAAAGGTTA TGCAGTACAC GGGATTAAGC GTCGCACCTC GCAATTTAAC ACAGCACGTA TCGATCATCT GTACCATGAT CCGCACGAGC AGGGTGTCGA TCTAACACTT CATCATGGCG ATCTAACAGA TACGTCAAGC TTGGTTCGAA TCATGCAGCT CGTTCGGCCG GATGAAGTTT ATAACCTTGG CGCACAAAGC CATGTTGCTG TTTCATTTGA GGAGCCGGAG TATACTGCGA ACTCAGACGC ACTAGGTGCC CTTCGTCTGC TAGAGGCGAT CCGAATTCTT GGAATGGAGA AGCAGACCCG GTACTACCAA GCATCCACCT CTGAGCTTTA TGGTCTGGTT CAAGAAATCC CGCAAAAAGA AACGACGCCT TTTTACCCTC GGTCGCCCTA TGCCGCAGCG AAGCTTTATG CCTATTGGAT TACGGTAAAC TACCGAGAGG CGTATGGTAT ATATGCTTGT AATGGCATAC TTTTTAACCA TGAATCTCCA CTTAGAGGAG AGACTTTTGT CACGAGAAAA ATTACTCGCG CTTTAGCTAG AATTAAGATC GGTAATCAGA ATCGCTTATA CTTAGGGAAT TTGGACTCAT TACGCGACTG GGGGCATGCT CGGGACTACG TCGAGATGCA GTGGTTAATG CTTCAACAGG ATCAACCAGA AGATTTTGTA ATAGCAACTG GTAAACAATA TTCAGTTCGA GAATTTGTCA CGCTCGCTGC TAAAGACATA GGCATTGAGA TGCGATGGGA AGGTAGCGGT GAAGCCGAGA AGGGTTATGA TGCCAAGACC GGTGCATGTA TTGTCGAGGT CGATCCTAGA TACTTTCGAC CAGCCGAGGT GGAAACTCTT CTGGGAGATG CGACCAAGGC GCATCAAAAA CTGGGCTGGA AGCCCAAAAT CTCTTTTGAG ACGCTTGTAT CTGAGATGAT AGCAGAAGAC TTAGCGGCGG CGGCTCGTGA GAATTTGCTG CGCGAACATG GTCACCAATT TTTCGCCCCT AAGGAA
Details for BrabA.00085.a.A1.GE25393
| HARVESTED ON: | 6/30/2008 |
| SEQUENCED ON: | 6/16/2009 |
| EXPECTED MW: | 43kDa |
| OBSERVED MW: | 44kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVKKVALIT GITGQDGAYL AELLLQKGYA VHGIKRRTSQ FNTARIDHLY HDPHEQGVDL TLHHGDLTDT SSLVRIMQLV RPDEVYNLGA QSHVAVSFEE PEYTANSDAL GALRLLEAIR ILGMEKQTRY YQASTSELYG LVQEIPQKET TPFYPRSPYA AAKLYAYWIT VNYREAYGIY ACNGILFNHE SPLRGETFVT RKITRALARI KIGNQNRLYL GNLDSLRDWG HARDYVEMQW LMLQQDQPED FVIATGKQYS VREFVTLAAK DIGIEMRWEG SGEAEKGYDA KTGACIVEVD PRYFRPAEVE TLLGDATKAH QKLGWKPKIS FETLVSEMIA EDLAAAAREN LLREHGHQFF AP
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga agaaagtcgc acttatcaca ggtatcaccg gccaagacgg cgcctacctt gcggaattgc ttctacaaaa aggttatgca gtacacggga ttaagcgtcg cacctcgcaa tttaacacag cacgtatcga tcatctgtac catgatccgc acgagcaggg tgtcgatcta acacttcatc atggcgatct aacagatacg tcaagcttgg ttcgaatcat gcagctcgtt cggccggatg aagtttataa ccttggcgca caaagccatg ttgctgtttc atttgaggag ccggagtata ctgcgaactc agacgcacta ggtgcccttc gtctgctaga ggcgatccga attcttggaa tggagaagca gacccggtac taccaagcat ccacctctga gctttatggt ctggttcaag aaatcccgca aaaagaaacg acgccttttt accctcggtc gccctatgcc gcagcgaagc tttatgccta ttggattacg gtaaactacc gagaggcgta tggtatatat gcttgtaatg gcatactttt taaccatgaa tctccactta gaggagagac ttttgtcacg agaaaaatta ctcgcgcttt agctagaatt aagatcggta atcagaatcg cttatactta gggaatttgg actcattacg cgactggggg catgctcggg actacgtcga gatgcagtgg ttaatgcttc aacaggatca accagaagat tttgtaatag caactggtaa acaatattca gttcgagaat ttgtcacgct cgctgctaaa gacataggca ttgagatgcg atgggaaggt agcggtgaag ccgagaaggg ttatgatgcc aagaccggtg catgtattgt cgaggtcgat cctagatact ttcgaccagc cgaggtggaa actcttctgg gagatgcgac caaggcgcat caaaaactgg gctggaagcc caaaatctct tttgagacgc ttgtatctga gatgatagca gaagacttag cggcggcggc tcgtgagaat ttgctgcgcg aacatggtca ccaatttttc gccccta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVKKVALIT GITGQDGAYL AELLLQKGYA VHGIKRRTSQ FNTARIDHLY HDPHEQGVDL TLHHGDLTDT SSLVRIMQLV RPDEVYNLGA QSHVAVSFEE PEYTANSDAL GALRLLEAIR ILGMEKQTRY YQASTSELYG LVQEIPQKET TPFYPRSPYA AAKLYAYWIT VNYREAYGIY ACNGILFNHE SPLRGETFVT RKITRALARI KIGNQNRLYL GNLDSLRDWG HARDYVEMQW LMLQQDQPED FVIATGKQYS VREFVTLAAK DIGIEMRWEG SGEAEKGYDA KTGACIVEVD PRYFRPAEVE TLLGDATKAH QKLGWKPKIS FETLVSEMIA EDLAAAAREN LLREHGHQFF APKE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaagaaa gtcgcactta tcacaggtat caccggccaa gacggcgcct accttgcgga attgcttcta caaaaaggtt atgcagtaca cgggattaag cgtcgcacct cgcaatttaa cacagcacgt atcgatcatc tgtaccatga tccgcacgag cagggtgtcg atctaacact tcatcatggc gatctaacag atacgtcaag cttggttcga atcatgcagc tcgttcggcc ggatgaagtt tataaccttg gcgcacaaag ccatgttgct gtttcatttg aggagccgga gtatactgcg aactcagacg cactaggtgc ccttcgtctg ctagaggcga tccgaattct tggaatggag aagcagaccc ggtactacca agcatccacc tctgagcttt atggtctggt tcaagaaatc ccgcaaaaag aaacgacgcc tttttaccct cggtcgccct atgccgcagc gaagctttat gcctattgga ttacggtaaa ctaccgagag gcgtatggta tatatgcttg taatggcata ctttttaacc atgaatctcc acttagagga gagacttttg tcacgagaaa aattactcgc gctttagcta gaattaagat cggtaatcag aatcgcttat acttagggaa tttggactca ttacgcgact gggggcatgc tcgggactac gtcgagatgc agtggttaat gcttcaacag gatcaaccag aagattttgt aatagcaact ggtaaacaat attcagttcg agaatttgtc acgctcgctg ctaaagacat aggcattgag atgcgatggg aaggtagcgg tgaagccgag aagggttatg atgccaagac cggtgcatgt attgtcgagg tcgatcctag atactttcga ccagccgagg tggaaactct tctgggagat gcgaccaagg cgcatcaaaa actgggctgg aagcccaaaa tctcttttga gacgcttgta tctgagatga tagcagaaga cttagcggcg gcggctcgtg agaatttgct gcgcgaacat ggtcaccaat ttttcgcccc taaggaatga aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for BrabA.00085.a.AE1.GE43128
| HARVESTED ON: | 5/17/2019 |
| SEQUENCED ON: | 5/24/2019 |
| EXPECTED MW: | 42kDa |
| OBSERVED MW: | 42kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MVKKVALITG ITGQDGAYLA ELLLQKGYAV HGIKRRTSQF NTARIDHLYH DPHEQGVDLT LHHGDLTDTS SLVRIMQLVR PDEVYNLGAQ SHVAVSFEEP EYTANSDALG ALRLLEAIRI LGMEKQTRYY QASTSELYGL VQEIPQKETT PFYPRSPYAA AKLYAYWITV NYREAYGIYA CNGILFNHES PLRGETFVTR KITRALARIK IGNQNRLYLG NLDSLRDWGH ARDYVEMQWL MLQQDQPEDF VIATGKQYSV XEFVTLAAKD IGIEMRWEGS GEAEKGYDAK TGACIVEVDP RYFRPAEVEX LLGDATKAHQ KLGWKPKISF ETLVSEMIAE DLAAAARENX LREHGHQFFA XKEXGHHHHH H
Validated NT Sequence
tccgttaatg atgatgatgg tggtgcccnn cttccttnnn ggcgaaaaat tggtgaccat gttcgcgcag naaattctca cgagccgccg ccgctaagtc ttctgctatc atctcagata caagcgtctc aaaagagatt ttgggcttcc agcccagttt ttgatgcgcc ttggtcgcat ctcccagaag agnttccacc tcggctggtc gaaagtatct aggatcgacc tcgacaatac atgcaccggt cttggcatca taacccttct cggcttcacc gctaccttcc catcgcatct caatgcctat gtctttagca gcgagcgtga caaattctng aactgaatat tgtttaccag ttgctattac aaaatcttct ggttgatcct gttgaagcat taaccactgc atctcgacgt agtcccgagc atgcccccag tcgcgtaatg agtccaaatt ccctaagtat aagcgattct gattaccgat cttaattcta gctaaagcgc gagtaatttt tctcgtgaca aaagtctctc ctctaagtgg agattcatgg ttaaaaagta tgccattaca agcatatata ccatacgcct ctcggtagtt taccgtaatc caataggcat aaagcttcgc tgcggcatag ggcgaccgag ggtaaaaagg cgtcgtttct ttttgcggga tttcttgaac cagaccataa agctcagagg tggatgcttg gtagtaccgg gtctgcttct ccattccaag aattcggatc gcctctagca gacgaagggc acctagtgcg tctgagttcg cagtatactc cggctcctca aatgaaacag caacatggct ttgtgcgcca aggttataaa cttcatccgg ccgaacgagc tgcatgattc gaaccaagct tgacgtatct gttagatcgc catgatgaag tgttagatcg acaccctgct cgtgcggatc atggtacaga tgatcgatac gtgctgtgtt aaattgcgag gtgcgacgct taatcccgtg tactgcataa cctttttgta gaagcaattc cgcaaggtag gcgccgtctt ggccggtgat acctgtgata agtgcgactt tcttcaccat gggagaga
Expected Protein Sequence
MKKVALITGI TGQDGAYLAE LLLQKGYAVH GIKRRTSQFN TARIDHLYHD PHEQGVDLTL HHGDLTDTSS LVRIMQLVRP DEVYNLGAQS HVAVSFEEPE YTANSDALGA LRLLEAIRIL GMEKQTRYYQ ASTSELYGLV QEIPQKETTP FYPRSPYAAA KLYAYWITVN YREAYGIYAC NGILFNHESP LRGETFVTRK ITRALARIKI GNQNRLYLGN LDSLRDWGHA RDYVEMQWLM LQQDQPEDFV IATGKQYSVR EFVTLAAKDI GIEMRWEGSG EAEKGYDAKT GACIVEVDPR YFRPAEVETL LGDATKAHQK LGWKPKISFE TLVSEMIAED LAAAARENLL REHGHQFFAP KEGHHHHHH
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccgt gaagaaagtc gcacttatca caggtatcac cggccaagac ggcgcctacc ttgcggaatt gcttctacaa aaaggttatg cagtacacgg gattaagcgt cgcacctcgc aatttaacac agcacgtatc gatcatctgt accatgatcc gcacgagcag ggtgtcgatc taacacttca tcatggcgat ctaacagata cgtcaagctt ggttcgaatc atgcagctcg ttcggccgga tgaagtttat aaccttggcg cacaaagcca tgttgctgtt tcatttgagg agccggagta tactgcgaac tcagacgcac taggtgccct tcgtctgcta gaggcgatcc gaattcttgg aatggagaag cagacccggt actaccaagc atccacctct gagctttatg gtctggttca agaaatcccg caaaaagaaa cgacgccttt ttaccctcgg tcgccctatg ccgcagcgaa gctttatgcc tattggatta cggtaaacta ccgagaggcg tatggtatat atgcttgtaa tggcatactt tttaaccatg aatctccact tagaggagag acttttgtca cgagaaaaat tactcgcgct ttagctagaa ttaagatcgg taatcagaat cgcttatact tagggaattt ggactcatta cgcgactggg ggcatgctcg ggactacgtc gagatgcagt ggttaatgct tcaacaggat caaccagaag attttgtaat agcaactggt aaacaatatt cagttcgaga atttgtcacg ctcgctgcta aagacatagg cattgagatg cgatgggaag gtagcggtga agccgagaag ggttatgatg ccaagaccgg tgcatgtatt gtcgaggtcg atcctagata ctttcgacca gccgaggtgg aaactcttct gggagatgcg accaaggcgc atcaaaaact gggctggaag cccaaaatct cttttgagac gcttgtatct gagatgatag cagaagactt agcggcggcg gctcgtgaga atttgctgcg cgaacatggt caccaatttt tcgcccctaa ggaagggcac caccatcatc atcattaacg gatccgaatt cgagctccgt cgacaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg at
Details for BrabA.00085.a.A1.PS00056
| PURIFICATION DATe: | 8/7/2008 |
| CONCENTRATION: | 48.45mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 15 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVKKVALIT GITGQDGAYL AELLLQKGYA VHGIKRRTSQ FNTARIDHLY HDPHEQGVDL TLHHGDLTDT SSLVRIMQLV RPDEVYNLGA QSHVAVSFEE PEYTANSDAL GALRLLEAIR ILGMEKQTRY YQASTSELYG LVQEIPQKET TPFYPRSPYA AAKLYAYWIT VNYREAYGIY ACNGILFNHE SPLRGETFVT RKITRALARI KIGNQNRLYL GNLDSLRDWG HARDYVEMQW LMLQQDQPED FVIATGKQYS VREFVTLAAK DIGIEMRWEG SGEAEKGYDA KTGACIVEVD PRYFRPAEVE TLLGDATKAH QKLGWKPKIS FETLVSEMIA EDLAAAAREN LLREHGHQFF AP
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga agaaagtcgc acttatcaca ggtatcaccg gccaagacgg cgcctacctt gcggaattgc ttctacaaaa aggttatgca gtacacggga ttaagcgtcg cacctcgcaa tttaacacag cacgtatcga tcatctgtac catgatccgc acgagcaggg tgtcgatcta acacttcatc atggcgatct aacagatacg tcaagcttgg ttcgaatcat gcagctcgtt cggccggatg aagtttataa ccttggcgca caaagccatg ttgctgtttc atttgaggag ccggagtata ctgcgaactc agacgcacta ggtgcccttc gtctgctaga ggcgatccga attcttggaa tggagaagca gacccggtac taccaagcat ccacctctga gctttatggt ctggttcaag aaatcccgca aaaagaaacg acgccttttt accctcggtc gccctatgcc gcagcgaagc tttatgccta ttggattacg gtaaactacc gagaggcgta tggtatatat gcttgtaatg gcatactttt taaccatgaa tctccactta gaggagagac ttttgtcacg agaaaaatta ctcgcgcttt agctagaatt aagatcggta atcagaatcg cttatactta gggaatttgg actcattacg cgactggggg catgctcggg actacgtcga gatgcagtgg ttaatgcttc aacaggatca accagaagat tttgtaatag caactggtaa acaatattca gttcgagaat ttgtcacgct cgctgctaaa gacataggca ttgagatgcg atgggaaggt agcggtgaag ccgagaaggg ttatgatgcc aagaccggtg catgtattgt cgaggtcgat cctagatact ttcgaccagc cgaggtggaa actcttctgg gagatgcgac caaggcgcat caaaaactgg gctggaagcc caaaatctct tttgagacgc ttgtatctga gatgatagca gaagacttag cggcggcggc tcgtgagaat ttgctgcgcg aacatggtca ccaatttttc gccccta
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVKKVALIT GITGQDGAYL AELLLQKGYA VHGIKRRTSQ FNTARIDHLY HDPHEQGVDL TLHHGDLTDT SSLVRIMQLV RPDEVYNLGA QSHVAVSFEE PEYTANSDAL GALRLLEAIR ILGMEKQTRY YQASTSELYG LVQEIPQKET TPFYPRSPYA AAKLYAYWIT VNYREAYGIY ACNGILFNHE SPLRGETFVT RKITRALARI KIGNQNRLYL GNLDSLRDWG HARDYVEMQW LMLQQDQPED FVIATGKQYS VREFVTLAAK DIGIEMRWEG SGEAEKGYDA KTGACIVEVD PRYFRPAEVE TLLGDATKAH QKLGWKPKIS FETLVSEMIA EDLAAAAREN LLREHGHQFF APKE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAAGAAA GTCGCACTTA TCACAGGTAT CACCGGCCAA GACGGCGCCT ACCTTGCGGA ATTGCTTCTA CAAAAAGGTT ATGCAGTACA CGGGATTAAG CGTCGCACCT CGCAATTTAA CACAGCACGT ATCGATCATC TGTACCATGA TCCGCACGAG CAGGGTGTCG ATCTAACACT TCATCATGGC GATCTAACAG ATACGTCAAG CTTGGTTCGA ATCATGCAGC TCGTTCGGCC GGATGAAGTT TATAACCTTG GCGCACAAAG CCATGTTGCT GTTTCATTTG AGGAGCCGGA GTATACTGCG AACTCAGACG CACTAGGTGC CCTTCGTCTG CTAGAGGCGA TCCGAATTCT TGGAATGGAG AAGCAGACCC GGTACTACCA AGCATCCACC TCTGAGCTTT ATGGTCTGGT TCAAGAAATC CCGCAAAAAG AAACGACGCC TTTTTACCCT CGGTCGCCCT ATGCCGCAGC GAAGCTTTAT GCCTATTGGA TTACGGTAAA CTACCGAGAG GCGTATGGTA TATATGCTTG TAATGGCATA CTTTTTAACC ATGAATCTCC ACTTAGAGGA GAGACTTTTG TCACGAGAAA AATTACTCGC GCTTTAGCTA GAATTAAGAT CGGTAATCAG AATCGCTTAT ACTTAGGGAA TTTGGACTCA TTACGCGACT GGGGGCATGC TCGGGACTAC GTCGAGATGC AGTGGTTAAT GCTTCAACAG GATCAACCAG AAGATTTTGT AATAGCAACT GGTAAACAAT ATTCAGTTCG AGAATTTGTC ACGCTCGCTG CTAAAGACAT AGGCATTGAG ATGCGATGGG AAGGTAGCGG TGAAGCCGAG AAGGGTTATG ATGCCAAGAC CGGTGCATGT ATTGTCGAGG TCGATCCTAG ATACTTTCGA CCAGCCGAGG TGGAAACTCT TCTGGGAGAT GCGACCAAGG CGCATCAAAA ACTGGGCTGG AAGCCCAAAA TCTCTTTTGA GACGCTTGTA TCTGAGATGA TAGCAGAAGA CTTAGCGGCG GCGGCTCGTG AGAATTTGCT GCGCGAACAT GGTCACCAAT TTTTCGCCCC TAAGGAATGA AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA
Details for BrabA.00085.a.A1.PS00349
| PURIFICATION DATe: | 9/8/2009 |
| CONCENTRATION: | 26.5mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVKKVALIT GITGQDGAYL AELLLQKGYA VHGIKRRTSQ FNTARIDHLY HDPHEQGVDL TLHHGDLTDT SSLVRIMQLV RPDEVYNLGA QSHVAVSFEE PEYTANSDAL GALRLLEAIR ILGMEKQTRY YQASTSELYG LVQEIPQKET TPFYPRSPYA AAKLYAYWIT VNYREAYGIY ACNGILFNHE SPLRGETFVT RKITRALARI KIGNQNRLYL GNLDSLRDWG HARDYVEMQW LMLQQDQPED FVIATGKQYS VREFVTLAAK DIGIEMRWEG SGEAEKGYDA KTGACIVEVD PRYFRPAEVE TLLGDATKAH QKLGWKPKIS FETLVSEMIA EDLAAAAREN LLREHGHQFF AP
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga agaaagtcgc acttatcaca ggtatcaccg gccaagacgg cgcctacctt gcggaattgc ttctacaaaa aggttatgca gtacacggga ttaagcgtcg cacctcgcaa tttaacacag cacgtatcga tcatctgtac catgatccgc acgagcaggg tgtcgatcta acacttcatc atggcgatct aacagatacg tcaagcttgg ttcgaatcat gcagctcgtt cggccggatg aagtttataa ccttggcgca caaagccatg ttgctgtttc atttgaggag ccggagtata ctgcgaactc agacgcacta ggtgcccttc gtctgctaga ggcgatccga attcttggaa tggagaagca gacccggtac taccaagcat ccacctctga gctttatggt ctggttcaag aaatcccgca aaaagaaacg acgccttttt accctcggtc gccctatgcc gcagcgaagc tttatgccta ttggattacg gtaaactacc gagaggcgta tggtatatat gcttgtaatg gcatactttt taaccatgaa tctccactta gaggagagac ttttgtcacg agaaaaatta ctcgcgcttt agctagaatt aagatcggta atcagaatcg cttatactta gggaatttgg actcattacg cgactggggg catgctcggg actacgtcga gatgcagtgg ttaatgcttc aacaggatca accagaagat tttgtaatag caactggtaa acaatattca gttcgagaat ttgtcacgct cgctgctaaa gacataggca ttgagatgcg atgggaaggt agcggtgaag ccgagaaggg ttatgatgcc aagaccggtg catgtattgt cgaggtcgat cctagatact ttcgaccagc cgaggtggaa actcttctgg gagatgcgac caaggcgcat caaaaactgg gctggaagcc caaaatctct tttgagacgc ttgtatctga gatgatagca gaagacttag cggcggcggc tcgtgagaat ttgctgcgcg aacatggtca ccaatttttc gccccta
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVKKVALIT GITGQDGAYL AELLLQKGYA VHGIKRRTSQ FNTARIDHLY HDPHEQGVDL TLHHGDLTDT SSLVRIMQLV RPDEVYNLGA QSHVAVSFEE PEYTANSDAL GALRLLEAIR ILGMEKQTRY YQASTSELYG LVQEIPQKET TPFYPRSPYA AAKLYAYWIT VNYREAYGIY ACNGILFNHE SPLRGETFVT RKITRALARI KIGNQNRLYL GNLDSLRDWG HARDYVEMQW LMLQQDQPED FVIATGKQYS VREFVTLAAK DIGIEMRWEG SGEAEKGYDA KTGACIVEVD PRYFRPAEVE TLLGDATKAH QKLGWKPKIS FETLVSEMIA EDLAAAAREN LLREHGHQFF APKE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAAGAAA GTCGCACTTA TCACAGGTAT CACCGGCCAA GACGGCGCCT ACCTTGCGGA ATTGCTTCTA CAAAAAGGTT ATGCAGTACA CGGGATTAAG CGTCGCACCT CGCAATTTAA CACAGCACGT ATCGATCATC TGTACCATGA TCCGCACGAG CAGGGTGTCG ATCTAACACT TCATCATGGC GATCTAACAG ATACGTCAAG CTTGGTTCGA ATCATGCAGC TCGTTCGGCC GGATGAAGTT TATAACCTTG GCGCACAAAG CCATGTTGCT GTTTCATTTG AGGAGCCGGA GTATACTGCG AACTCAGACG CACTAGGTGC CCTTCGTCTG CTAGAGGCGA TCCGAATTCT TGGAATGGAG AAGCAGACCC GGTACTACCA AGCATCCACC TCTGAGCTTT ATGGTCTGGT TCAAGAAATC CCGCAAAAAG AAACGACGCC TTTTTACCCT CGGTCGCCCT ATGCCGCAGC GAAGCTTTAT GCCTATTGGA TTACGGTAAA CTACCGAGAG GCGTATGGTA TATATGCTTG TAATGGCATA CTTTTTAACC ATGAATCTCC ACTTAGAGGA GAGACTTTTG TCACGAGAAA AATTACTCGC GCTTTAGCTA GAATTAAGAT CGGTAATCAG AATCGCTTAT ACTTAGGGAA TTTGGACTCA TTACGCGACT GGGGGCATGC TCGGGACTAC GTCGAGATGC AGTGGTTAAT GCTTCAACAG GATCAACCAG AAGATTTTGT AATAGCAACT GGTAAACAAT ATTCAGTTCG AGAATTTGTC ACGCTCGCTG CTAAAGACAT AGGCATTGAG ATGCGATGGG AAGGTAGCGG TGAAGCCGAG AAGGGTTATG ATGCCAAGAC CGGTGCATGT ATTGTCGAGG TCGATCCTAG ATACTTTCGA CCAGCCGAGG TGGAAACTCT TCTGGGAGAT GCGACCAAGG CGCATCAAAA ACTGGGCTGG AAGCCCAAAA TCTCTTTTGA GACGCTTGTA TCTGAGATGA TAGCAGAAGA CTTAGCGGCG GCGGCTCGTG AGAATTTGCT GCGCGAACAT GGTCACCAAT TTTTCGCCCC TAAGGAATGA AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA
Details for BrabA.00085.a.AE1.PW38674
| PURIFICATION DATe: | 7/28/2019 |
| CONCENTRATION: | 58.8mg/ml |
| OBSERVED MW: | 40kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 10 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MVKKVALITG ITGQDGAYLA ELLLQKGYAV HGIKRRTSQF NTARIDHLYH DPHEQGVDLT LHHGDLTDTS SLVRIMQLVR PDEVYNLGAQ SHVAVSFEEP EYTANSDALG ALRLLEAIRI LGMEKQTRYY QASTSELYGL VQEIPQKETT PFYPRSPYAA AKLYAYWITV NYREAYGIYA CNGILFNHES PLRGETFVTR KITRALARIK IGNQNRLYLG NLDSLRDWGH ARDYVEMQWL MLQQDQPEDF VIATGKQYSV XEFVTLAAKD IGIEMRWEGS GEAEKGYDAK TGACIVEVDP RYFRPAEVEX LLGDATKAHQ KLGWKPKISF ETLVSEMIAE DLAAAARENX LREHGHQFFA XKEXGHHHHH H
Validated NT Sequence
tccgttaatg atgatgatgg tggtgcccnn cttccttnnn ggcgaaaaat tggtgaccat gttcgcgcag naaattctca cgagccgccg ccgctaagtc ttctgctatc atctcagata caagcgtctc aaaagagatt ttgggcttcc agcccagttt ttgatgcgcc ttggtcgcat ctcccagaag agnttccacc tcggctggtc gaaagtatct aggatcgacc tcgacaatac atgcaccggt cttggcatca taacccttct cggcttcacc gctaccttcc catcgcatct caatgcctat gtctttagca gcgagcgtga caaattctng aactgaatat tgtttaccag ttgctattac aaaatcttct ggttgatcct gttgaagcat taaccactgc atctcgacgt agtcccgagc atgcccccag tcgcgtaatg agtccaaatt ccctaagtat aagcgattct gattaccgat cttaattcta gctaaagcgc gagtaatttt tctcgtgaca aaagtctctc ctctaagtgg agattcatgg ttaaaaagta tgccattaca agcatatata ccatacgcct ctcggtagtt taccgtaatc caataggcat aaagcttcgc tgcggcatag ggcgaccgag ggtaaaaagg cgtcgtttct ttttgcggga tttcttgaac cagaccataa agctcagagg tggatgcttg gtagtaccgg gtctgcttct ccattccaag aattcggatc gcctctagca gacgaagggc acctagtgcg tctgagttcg cagtatactc cggctcctca aatgaaacag caacatggct ttgtgcgcca aggttataaa cttcatccgg ccgaacgagc tgcatgattc gaaccaagct tgacgtatct gttagatcgc catgatgaag tgttagatcg acaccctgct cgtgcggatc atggtacaga tgatcgatac gtgctgtgtt aaattgcgag gtgcgacgct taatcccgtg tactgcataa cctttttgta gaagcaattc cgcaaggtag gcgccgtctt ggccggtgat acctgtgata agtgcgactt tcttcaccat gggagaga
Expressed Protein Sequence
MKKVALITGI TGQDGAYLAE LLLQKGYAVH GIKRRTSQFN TARIDHLYHD PHEQGVDLTL HHGDLTDTSS LVRIMQLVRP DEVYNLGAQS HVAVSFEEPE YTANSDALGA LRLLEAIRIL GMEKQTRYYQ ASTSELYGLV QEIPQKETTP FYPRSPYAAA KLYAYWITVN YREAYGIYAC NGILFNHESP LRGETFVTRK ITRALARIKI GNQNRLYLGN LDSLRDWGHA RDYVEMQWLM LQQDQPEDFV IATGKQYSVR EFVTLAAKDI GIEMRWEGSG EAEKGYDAKT GACIVEVDPR YFRPAEVETL LGDATKAHQK LGWKPKISFE TLVSEMIAED LAAAARENLL REHGHQFFAP KEGHHHHHH
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACATTAACGC TTACAATTTA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATTTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAG ATCCCGGTGC CTAATGAGTG AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA GATTGTGCAC CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC GACACCACCA CGCTGGCACC CAGTTGATCG GCGCGAGATT TAATCGCCGC GACAATTTGC GACGGCGCGT GCAGGGCCAG ACTGGAGGTG GCAACGCCAA TCAGCAACGA CTGTTTGCCC GCCAGTTGTT GTGCCACGCG GTTGGGAATG TAATTCAGCT CCGCCATCGC CGCTTCCACT TTTTCCCGCG TTTTCGCAGA AACGTGGCTG GCCTGGTTCA CCACGCGGGA AACGGTCTGA TAAGAGACAC CGGCATACTC TGCGACATCG TATAACGTTA CTGGTTTCAC ATTCACCACC CTGAATTGAC TCTCTTCCGG GCGCTATCAT GCCATACCGC GAAAGGTTTT GCGCCATTCG ATGGTGTCCG GGATCTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA GAAATAATTT TGTTTAACTT TAAGAAGGAG TCTCTCCCGT GAAGAAAGTC GCACTTATCA CAGGTATCAC CGGCCAAGAC GGCGCCTACC TTGCGGAATT GCTTCTACAA AAAGGTTATG CAGTACACGG GATTAAGCGT CGCACCTCGC AATTTAACAC AGCACGTATC GATCATCTGT ACCATGATCC GCACGAGCAG GGTGTCGATC TAACACTTCA TCATGGCGAT CTAACAGATA CGTCAAGCTT GGTTCGAATC ATGCAGCTCG TTCGGCCGGA TGAAGTTTAT AACCTTGGCG CACAAAGCCA TGTTGCTGTT TCATTTGAGG AGCCGGAGTA TACTGCGAAC TCAGACGCAC TAGGTGCCCT TCGTCTGCTA GAGGCGATCC GAATTCTTGG AATGGAGAAG CAGACCCGGT ACTACCAAGC ATCCACCTCT GAGCTTTATG GTCTGGTTCA AGAAATCCCG CAAAAAGAAA CGACGCCTTT TTACCCTCGG TCGCCCTATG CCGCAGCGAA GCTTTATGCC TATTGGATTA CGGTAAACTA CCGAGAGGCG TATGGTATAT ATGCTTGTAA TGGCATACTT TTTAACCATG AATCTCCACT TAGAGGAGAG ACTTTTGTCA CGAGAAAAAT TACTCGCGCT TTAGCTAGAA TTAAGATCGG TAATCAGAAT CGCTTATACT TAGGGAATTT GGACTCATTA CGCGACTGGG GGCATGCTCG GGACTACGTC GAGATGCAGT GGTTAATGCT TCAACAGGAT CAACCAGAAG ATTTTGTAAT AGCAACTGGT AAACAATATT CAGTTCGAGA ATTTGTCACG CTCGCTGCTA AAGACATAGG CATTGAGATG CGATGGGAAG GTAGCGGTGA AGCCGAGAAG GGTTATGATG CCAAGACCGG TGCATGTATT GTCGAGGTCG ATCCTAGATA CTTTCGACCA GCCGAGGTGG AAACTCTTCT GGGAGATGCG ACCAAGGCGC ATCAAAAACT GGGCTGGAAG CCCAAAATCT CTTTTGAGAC GCTTGTATCT GAGATGATAG CAGAAGACTT AGCGGCGGCG GCTCGTGAGA ATTTGCTGCG CGAACATGGT CACCAATTTT TCGCCCCTAA GGAAGGGCAC CACCATCATC ATCATTAACG GATCCGAATT CGAGCTCCGT CGACAAGCTT GCGGCCGCAC TCGAGCACCA CCACCACCAC CACTGAGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG AT