BrabA.00070.a
AP endonuclease, family 2
| CENTER ID: | BrabA.00070.a |
| ORGANISM: | Brucella melitensis biovar Abortus 2308 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
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Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.00070.a.A1.PW25424 | Full length( BrabA.00070.a ) | 1 | 261 | ||
| BrabA.00070.a.B1.PW25525 | Full length( BrabA.00070.a ) | 1 | 261 |
Structures
3NGF
DEPOSITED: 6/11/2010
DETERMINATION: XRay
CLONE: BrabA.00070.a.B1.GE25482
PROTEIN: BrabA.00070.a.B1.PW25525
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_131199 |
| PATRIC ID: | fig|359391.4.peg.2421 |
| RefSeq: | YP_418380 |
| UniProt: | Q2YLA2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPRFAANLST MFNEVPFLER FRLAAEAGFG GVEFLFPYDF DADVIARELK QHNLTQVLFN MPPGDWAAGE RGMAAISGRE QEFRDNVDIA LHYALALDCR TLHAMSGITE GLDRKACEET FIENFRYAAD KLAPHGITVL VEPLNTRNMP GYFIVHQLEA VGLVKRVNRP NVAVQLDLYH AQIMDGDLTR LIEKMNGAFS HVQIASVPDR HEPDEGELNY PYLFSVLESV GYRGWVGCEY NPRGKTESGL AWFAPYRDQS A
NT Sequence
ATGCCGCGTT TTGCTGCCAA TCTGTCGACG ATGTTTAACG AGGTGCCATT CCTCGAACGT TTTAGACTGG CTGCCGAAGC AGGTTTTGGC GGCGTCGAGT TTCTGTTTCC TTATGACTTC GACGCTGATG TCATTGCCAG GGAATTGAAG CAGCACAATC TCACGCAGGT TCTTTTCAAC ATGCCTCCCG GCGATTGGGC TGCAGGCGAA CGCGGCATGG CAGCTATTTC GGGTCGCGAA CAGGAATTCC GCGACAATGT CGATATCGCC CTGCATTATG CATTGGCGCT GGATTGCAGG ACGCTTCACG CCATGTCCGG CATCACGGAA GGGCTGGACC GGAAAGCCTG CGAGGAAACC TTTATCGAAA ACTTCCGCTA TGCGGCAGAC AAGCTTGCTC CGCACGGCAT CACGGTGCTG GTCGAGCCCC TCAATACGCG CAATATGCCC GGCTATTTCA TCGTGCATCA ACTGGAAGCC GTTGGCCTCG TAAAGAGGGT CAACCGCCCC AATGTCGCCG TGCAGCTCGA TCTCTATCAT GCCCAGATCA TGGATGGCGA CCTGACCCGC CTGATCGAGA AAATGAACGG TGCATTCAGC CATGTTCAAA TTGCATCCGT TCCCGACCGG CATGAGCCGG ATGAAGGCGA ATTGAACTAT CCATATCTTT TCTCGGTCCT GGAGAGTGTC GGCTATCGGG GATGGGTGGG ATGCGAATAT AATCCGCGGG GCAAAACGGA AAGCGGCCTC GCCTGGTTTG CCCCATACCG GGATCAAAGC GCA
Details for BrabA.00070.a.A1.PW25424
| PURIFICATION DATe: | 3/8/2010 |
| CONCENTRATION: | 20.3mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 20 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 93 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPRFAANLS TMFNEVPFLE RFRLAAEAGF GGVEFLFPYD FDADVIAREL KQHNLTQVLF NMPPGDWAAG ERGMAAISGR EQEFRDNVDI ALHYALALDC RTLHAMSGIT EGLDRKACEE TFIENFRYAA DKLAPHGITV LVEPLNTRNM PGYFIVHQLE AVGLVKRVNR PNVAVQLDLY HAQIMDGDLT RLIEKMNGAF SHVQIASVPD RHEPDEGELX YPYLFSVLES VGYRGXVGCE YNPA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgccgc gttttgctgc caatctgtcg acgatgttta acgaggtgcc attcctcgaa cgttttagac tggctgccga agcaggtttt ggcggcgtcg agtttctgtt tccttatgac ttcgacgctg atgtcattgc cagggaattg aagcagcaca atctcacgca ggttcttttc aacatgcctc ccggcgattg ggctgcaggc gaacgcggca tggcagctat ttcgggtcgc gaacaggaat tccgcgacaa tgtcgatatc gccctgcatt atgcattggc gctggattgc aggacgcttc acgccatgtc cggcatcacg gaagggctgg accggaaagc ctgcgaggaa acctttatcg aaaacttccg ctatgcggca gacaagcttg ctccgcacgg catcacggtg ctggtcgagc ccctcaatac gcgcaatatg cccggctatt tcatcgtgca tcaactggaa gccgttggcc tcgtaaagag ggtcaaccgc cccaatgtcg ccgtgcagct cgatctctat catgcccaga tcatggatgg cgacctgacc cgcctgatcg agaaaatgaa cggtgcattc agccatgttc aaattgcatc cgtncccgac cggcatgagc cggatgaagg cgaattgaan tatccatatc ttttctcggt cctggagagt gtcggctatc ggggangggt gggatgcgaa tataatccng cgggg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPRFAANLS TMFNEVPFLE RFRLAAEAGF GGVEFLFPYD FDADVIAREL KQHNLTQVLF NMPPGDWAAG ERGMAAISGR EQEFRDNVDI ALHYALALDC RTLHAMSGIT EGLDRKACEE TFIENFRYAA DKLAPHGITV LVEPLNTRNM PGYFIVHQLE AVGLVKRVNR PNVAVQLDLY HAQIMDGDLT RLIEKMNGAF SHVQIASVPD RHEPDEGELN YPYLFSVLES VGYRGWVGCE YNPRGKTESG LAWFAPYRDQ SA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCGCGTTTT GCTGCCAATC TGTCGACGAT GTTTAACGAG GTGCCATTCC TCGAACGTTT TAGACTGGCT GCCGAAGCAG GTTTTGGCGG CGTCGAGTTT CTGTTTCCTT ATGACTTCGA CGCTGATGTC ATTGCCAGGG AATTGAAGCA GCACAATCTC ACGCAGGTTC TTTTCAACAT GCCTCCCGGC GATTGGGCTG CAGGCGAACG CGGCATGGCA GCTATTTCGG GTCGCGAACA GGAATTCCGC GACAATGTCG ATATCGCCCT GCATTATGCA TTGGCGCTGG ATTGCAGGAC GCTTCACGCC ATGTCCGGCA TCACGGAAGG GCTGGACCGG AAAGCCTGCG AGGAAACCTT TATCGAAAAC TTCCGCTATG CGGCAGACAA GCTTGCTCCG CACGGCATCA CGGTGCTGGT CGAGCCCCTC AATACGCGCA ATATGCCCGG CTATTTCATC GTGCATCAAC TGGAAGCCGT TGGCCTCGTA AAGAGGGTCA ACCGCCCCAA TGTCGCCGTG CAGCTCGATC TCTATCATGC CCAGATCATG GATGGCGACC TGACCCGCCT GATCGAGAAA ATGAACGGTG CATTCAGCCA TGTTCAAATT GCATCCGTTC CCGACCGGCA TGAGCCGGAT GAAGGCGAAT TGAACTATCC ATATCTTTTC TCGGTCCTGG AGAGTGTCGG CTATCGGGGA TGGGTGGGAT GCGAATATAA TCCGCGGGGC AAAACGGAAA GCGGCCTCGC CTGGTTTGCC CCATACCGGG ATCAAAGCGC ATGAAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA
Details for BrabA.00070.a.B1.PW25525
| PURIFICATION DATe: | 4/19/2010 |
| CONCENTRATION: | 27.33mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 13 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MPRFAANLST MFNEVPFLER FRLAAEAGFG GVEFLFPYDF DADVIARELK QHNLTQVLFN MPPGDWAAGE RGMAAISGRE QEFRDNVDIA LHYALALDCR TLHAMSGITE GLDRKACEET FIENFRYAAD KLAPHGITVL VEPLNTRNMP GYFIVHQLEA VGLVKRVNRP NVAVQLDLYH AQIMDGDLTR LIEKMNGAFS HVQIASVPDR HEPDEGELNY PYLFSVLESV GYRGWVGCEY NPRGKTESGL AWFAPYRDQS A
Validated NT Sequence
catatgccgc gttttgctgc caatctgtcg acgatgttta acgaggtgcc attcctcgaa cgttttagac tggctgccga agcaggtttt ggcggcgtcg agtttctgtt tccttatgac ttcgacgctg atgtcattgc cagggaattg aagcagcaca atctcacgca ggttcttttc aacatgcctc ccggcgattg ggctgcaggc gaacgcggca tggcagctat ttcgggtcgc gaacaggaat tccgcgacaa tgtcgatatc gccctgcatt atgcattggc gctggattgc aggacgcttc acgccatgtc cggcatcacg gaagggctgg accggaaagc ctgcgaggaa acctttatcg aaaacttccg ctatgcggca gacaagcttg ctccgcacgg catcacggtg ctggtcgagc ccctcaatac gcgcaatatg cccggctatt tcatcgtgca tcaactggaa gccgttggcc tcgtaaagag ggtcaaccgc cccaatgtcg ccgtgcagct cgatctctat catgcccaga tcatggatgg cgacctgacc cgcctgatcg agaaaatgaa cggtgcattc agccatgttc aaattgcatc cgttcccgac cggcatgagc cggatgaagg cgaattgaac tatccatatc ttttctcggt cctggagagt gtcggctatc ggggatgggt gggatgcgaa tataatccgc ggggcaaaac ggaaagcggc ctcgcctggt ttgccccata ccgggatcaa agcgcataag tgagtaagat
Expressed Protein Sequence
MAHHHHHHMP RFAANLSTMF NEVPFLERFR LAAEAGFGGV EFLFPYDFDA DVIARELKQH NLTQVLFNMP PGDWAAGERG MAAISGREQE FRDNVDIALH YALALDCRTL HAMSGITEGL DRKACEETFI ENFRYAADKL APHGITVLVE PLNTRNMPGY FIVHQLEAVG LVKRVNRPNV AVQLDLYHAQ IMDGDLTRLI EKMNGAFSHV QIASVPDRHE PDEGELNYPY LFSVLESVGY RGWVGCEYNP RGKTESGLAW FAPYRDQSA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCGC GTTTTGCTGC CAATCTGTCG ACGATGTTTA ACGAGGTGCC ATTCCTCGAA CGTTTTAGAC TGGCTGCCGA AGCAGGTTTT GGCGGCGTCG AGTTTCTGTT TCCTTATGAC TTCGACGCTG ATGTCATTGC CAGGGAATTG AAGCAGCACA ATCTCACGCA GGTTCTTTTC AACATGCCTC CCGGCGATTG GGCTGCAGGC GAACGCGGCA TGGCAGCTAT TTCGGGTCGC GAACAGGAAT TCCGCGACAA TGTCGATATC GCCCTGCATT ATGCATTGGC GCTGGATTGC AGGACGCTTC ACGCCATGTC CGGCATCACG GAAGGGCTGG ACCGGAAAGC CTGCGAGGAA ACCTTTATCG AAAACTTCCG CTATGCGGCA GACAAGCTTG CTCCGCACGG CATCACGGTG CTGGTCGAGC CCCTCAATAC GCGCAATATG CCCGGCTATT TCATCGTGCA TCAACTGGAA GCCGTTGGCC TCGTAAAGAG GGTCAACCGC CCCAATGTCG CCGTGCAGCT CGATCTCTAT CATGCCCAGA TCATGGATGG CGACCTGACC CGCCTGATCG AGAAAATGAA CGGTGCATTC AGCCATGTTC AAATTGCATC CGTTCCCGAC CGGCATGAGC CGGATGAAGG CGAATTGAAC TATCCATATC TTTTCTCGGT CCTGGAGAGT GTCGGCTATC GGGGATGGGT GGGATGCGAA TATAATCCGC GGGGCAAAAC GGAAAGCGGC CTCGCCTGGT TTGCCCCATA CCGGGATCAA AGCGCATGAT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT