BrabA.00060.a

3' exoribonuclease:Ribonuclease PH

CENTER ID:	BrabA.00060.a
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.00060.a.A1.GE25223	Full length( BrabA.00060.a )		1	238

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_127234
PATRIC ID:	fig\|359391.4.peg.182
RefSeq:	YP_413662
UniProt:	Q2YP33

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRPSKRAADE MRAISFERGV SKHAEGSCLV KFGDTHVLCT ASLEEKVPGW MRNTGKGWVT AEYGMLPRST GERMRREAAA GKQGGRTQEI QRLIGRSLRA VVDMQALGEM QITVDCDVIQ ADGGTRTAAI TGGWVALHEC LRWMEARQMV RVEKVLKDHV AAISCGIYEG VPVLDLDYAE DSVAETDSNF VMTGKGGIVE IQGTAEGVPF SEEEFGALMK LARSGIDRLV SLQKMAVA

NT Sequence

ATGCGTCCAT CCAAGCGTGC TGCCGACGAA ATGCGCGCCA TCTCTTTCGA GCGCGGCGTC TCCAAACATG CGGAAGGCTC CTGCCTGGTC AAGTTTGGCG ACACCCATGT TTTGTGCACG GCAAGCCTTG AGGAAAAAGT GCCGGGCTGG ATGCGCAATA CGGGCAAGGG CTGGGTGACG GCGGAATATG GTATGCTGCC GCGTTCCACC GGCGAGCGGA TGCGCCGCGA GGCGGCGGCG GGCAAGCAGG GCGGGCGCAC GCAGGAAATC CAGCGCCTCA TCGGCCGCTC GCTCCGCGCG GTCGTGGACA TGCAGGCGCT TGGCGAAATG CAGATCACGG TGGACTGCGA TGTGATCCAG GCCGATGGCG GCACGCGCAC GGCGGCTATC ACCGGCGGCT GGGTGGCGCT GCACGAATGC CTGCGCTGGA TGGAAGCGCG CCAGATGGTG CGTGTCGAAA AGGTCCTGAA AGACCATGTA GCCGCCATTT CCTGCGGCAT TTATGAAGGC GTGCCGGTTC TCGATCTCGA CTATGCCGAG GATTCGGTTG CCGAAACCGA CAGCAATTTC GTCATGACCG GCAAGGGCGG CATCGTGGAA ATTCAGGGCA CCGCCGAAGG CGTGCCTTTC TCGGAAGAGG AGTTTGGCGC GCTGATGAAG CTTGCGCGCA GCGGCATCGA TCGTCTCGTA TCGTTGCAGA AAATGGCCGT CGCC

Details for BrabA.00060.a.A1.GE25223

HARVESTED ON:	5/6/2008
SEQUENCED ON:	6/16/2009
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	97
PERCENT COVERAGE:	90

Validated AA Sequence

MRPSKRAADE MRAISFERGV SKHAEGSCLV KFGDTHVLCT ASLEEKVPGW MRNTGKGWVT AEYGMLPRST GERMRREAAA GKQGGRTQEI QRLIGRSLRA VVDMQALGEM QITVDCDVIQ ADGGTRTAAI TGGWVALHEC LRWMEARQMV RVEKVLKDHV AAISCGIYEG VPVLDLDYAE XSVAETDSNF VMTGKGGIVE IQGTAXGVPF SEXXFGALMK LARSXIDRLV SLQK

Validated NT Sequence

caccatcacc atcanntggg taccctggaa gctcagaccc agggtcctgg ttcgatgcgt ccatccaagc gtgctgccga cgaaatgcgc gccatctctt tcgagcgcgg cgtctccaaa catgcggaag gctcctgcct ggtcaagttt ggcgacaccc atgttttgtg cacggcaagc cttgaggaaa aagtgccggg ctggatgcgc aatacgggca agggctgggt gacggcggaa tatggtatgc tgccgcgttc caccggcgag cggatgcgcc gcgaggcggc ggcgggcaag cagggcgggc gcacgcagga aatccagcgc ctcatcggcc gctcgctccg cgcggtcgtg gacatgcagg cgcttggcga aatgcagatc acggtggact gcgatgtgat ccaggccgat ggcggcacgc gcacggcggc tatcaccggc ggctgggtgg cgctgcacga atgcctgcgc tggatggaag cgcgccagat ggtgcgtgtc gaaaaggtcc tgaaagacca tgtagccgcc atttcctgcg gcatttatga aggcgtgccg gttctcgatc tcgactatgc cgaggantcg gttgccgaaa ccgacagcaa tttcgtcatg accggcaagg gcggcatcgt ggaaattcag ggcaccgccn aaggcgtgcc tttctcggaa gangantttg gcgcgctgat gaagctggcg cgcagcgnca tcgatcgtct cgtatcgttg cagaaaatg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMRPSKRAAD EMRAISFERG VSKHAEGSCL VKFGDTHVLC TASLEEKVPG WMRNTGKGWV TAEYGMLPRS TGERMRREAA AGKQGGRTQE IQRLIGRSLR AVVDMQALGE MQITVDCDVI QADGGTRTAA ITGGWVALHE CLRWMEARQM VRVEKVLKDH VAAISCGIYE GVPVLDLDYA EDSVAETDSN FVMTGKGGIV EIQGTAEGVP FSEEEFGALM KLARSGIDRL VSLQKMAVA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgtccatcc aagcgtgctg ccgacgaaat gcgcgccatc tctttcgagc gcggcgtctc caaacatgcg gaaggctcct gcctggtcaa gtttggcgac acccatgttt tgtgcacggc aagccttgag gaaaaagtgc cgggctggat gcgcaatacg ggcaagggct gggtgacggc ggaatatggt atgctgccgc gttccaccgg cgagcggatg cgccgcgagg cggcggcggg caagcagggc gggcgcacgc aggaaatcca gcgcctcatc ggccgctcgc tccgcgcggt cgtggacatg caggcgcttg gcgaaatgca gatcacggtg gactgcgatg tgatccaggc cgatggcggc acgcgcacgg cggctatcac cggcggctgg gtggcgctgc acgaatgcct gcgctggatg gaagcgcgcc agatggtgcg tgtcgaaaag gtcctgaaag accatgtagc cgccatttcc tgcggcattt atgaaggcgt gccggttctc gatctcgact atgccgagga ttcggttgcc gaaaccgaca gcaatttcgt catgaccggc aagggcggca tcgtggaaat tcagggcacc gccgaaggcg tgcctttctc ggaagaggag tttggcgcgc tgatgaagct ggcgcgcagc ggcatcgatc gtctcgtatc gttgcagaaa atggccgtcg cctgaaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa