BrabA.00057.o

Glycerol-3-phosphate ABC transporter, ATP-binding protein UgpC (TC 3.A.1.1.3)

CENTER ID: BrabA.00057.o
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.00057.o.B1.GE37705 Full length( BrabA.00057.o ) 1 392
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruMel86222_3448
BV-BRC: fig|359391.4.peg.3448
RefSeq: YP_419265.1
UniProt: Q2YJB9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTLVLRDIG KSYGGNRVLD GISIEAAEGE FIALVGPSGC GKSTLLRILA GLDHADEGSV ELAGCNITGA HPADRNIAMV FQSYALYPHL TAAQNIAVPL AMRDLTVMER LPVIGAFLPG QRDKRAAIQK QVRATAASLK IAPLLDRKPG QMSGGQRQRV ALARALVRQP GAFLMDEPLS NLDANLRVHT RAEIVELHRR AGVPTIYVTH DQAEALSMAD RVAVMMGGKL LQIDTPGAIY NEPRHIEVAQ FIGTPRINIL EALVSDNGVV HFVGRAILQG VAAHSGSNIR IGIRPEHFQL RPHDKTGIAS TVSRIEFLGS EVLAYLKVPG AGQDLTVKLS PEDARDIVVG LPAGLHFDAS KAYLFGEGGL RLRQPAGNIV KLASPAKATA HV
NT Sequence
GTGAGCACAC TCGTCCTTCG CGACATTGGC AAATCCTACG GGGGAAATCG CGTTCTCGAT GGCATTTCGA TCGAAGCCGC AGAAGGCGAA TTCATCGCCC TCGTCGGCCC GTCCGGCTGC GGCAAGAGCA CATTGCTGCG TATTCTTGCC GGGCTCGATC ATGCGGACGA AGGAAGCGTG GAGCTTGCCG GGTGCAACAT AACCGGCGCC CACCCCGCCG ACCGGAACAT TGCTATGGTG TTCCAGTCCT ATGCACTCTA TCCCCATCTG ACAGCGGCGC AGAACATCGC CGTGCCGCTG GCGATGCGCG ATCTGACCGT AATGGAACGC CTGCCGGTGA TCGGCGCCTT CCTGCCCGGC CAGCGCGACA AGCGCGCCGC CATCCAGAAG CAGGTCAGGG CAACCGCGGC CAGCCTCAAG ATCGCCCCCC TGCTCGACCG CAAGCCGGGC CAGATGTCCG GTGGCCAGCG CCAGCGTGTG GCATTGGCCC GCGCACTTGT CCGCCAGCCC GGCGCATTTC TGATGGACGA ACCGCTGTCC AATCTCGATG CCAATCTGCG CGTTCACACG CGCGCAGAAA TCGTCGAACT GCATCGCCGC GCCGGTGTGC CCACCATCTA TGTGACCCAC GATCAGGCTG AAGCACTCAG CATGGCCGAC CGCGTTGCGG TGATGATGGG CGGCAAACTG CTCCAGATCG ACACGCCTGG CGCGATCTAT AACGAGCCGC GCCATATCGA GGTCGCGCAA TTCATCGGCA CGCCGCGCAT CAATATTCTG GAAGCATTGG TCAGCGATAA CGGCGTGGTG CATTTTGTAG GCCGCGCCAT CCTTCAGGGC GTCGCCGCCC ATTCCGGCAG CAATATTCGT ATTGGCATTC GTCCTGAACA TTTTCAGCTC AGGCCGCACG ACAAGACAGG CATTGCAAGC ACCGTTTCAC GGATCGAATT CCTCGGCTCG GAAGTCCTTG CCTATCTCAA GGTCCCCGGC GCCGGGCAGG ATCTCACGGT GAAGCTTTCA CCGGAAGACG CCCGCGACAT CGTCGTCGGC CTGCCTGCGG GTCTGCATTT TGATGCCAGC AAGGCTTATC TCTTCGGGGA AGGTGGCCTG CGCCTGCGCC AGCCCGCCGG AAATATCGTG AAGCTTGCAT CCCCGGCAAA GGCTACCGCT CATGTC
Details for BrabA.00057.o.B1.GE37705
HARVESTED ON: 2/5/2014
SEQUENCED ON: 2/6/2014
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 85
PERCENT COVERAGE: 90
Validated AA Sequence
MAHHHHHHMV STLVLRDIGK SYGGNRVLDG ISIEAAEGEF IALVGPSGCG KSTLLRILAG LDHADEGSVE LAGCNITGAH PADRNIAMVF QSYALYPHLT AAQNIAVPLA MRDLTVMERL PVIGAFLPGQ RDKRAAIQKQ VRATAASLKI APLLDRKPGQ MSGGQRQRVA LARALVRQPG AFLMDEPLSN LDANLRVHTR AEIVELHRRA GVPTIYVTHD QAEALSMADR VAVMMGGKLL QIDTPGAIYN EPRHIEVAQF IGTPRINILE ALVSDNGVVH FVAAPSFXRR RPFRQHIRIG IRPEHFQLXR TTDXMQAPFH DRXXXEVLAY LKSXXXXLTX ASXXXXXXXG XXXWXLXQQA X
Validated NT Sequence
cnnnngnann ncnctagaan attttgttta actttaagaa ggagatatac catggctcac caccaccacc accatatggt gagcacactc gtccttcgcg acattggcaa atcctacggg ggaaatcgcg ttctcgatgg catttcgatc gaagccgcag aaggcgaatt catcgccctc gtcggcccgt ccggctgcgg caagagcaca ttgctgcgta ttcttgccgg gctcgatcat gcggacgaag gaagcgtgga gcttgccggg tgcaacataa ccggcgccca ccccgccgac cggaacattg ctatggtgtt ccagtcctat gcactctatc cccatctgac agcggcgcag aacatcgccg tgccgctggc gatgcgcgat ctgaccgtaa tggaacgcct gccggtgatc ggcgccttcc tgcccggcca gcgcgacaag cgcgccgcca tccagaagca ggtcagggca accgcggcca gcctcaagat cgcccccctg ctcgaccgca agccgggcca gatgtccggt ggccagcgcc agcgtgtggc attggcccgc gcacttgtcc gccagcccgg cgcatttctg atggacgaac cgctgtccaa tctcgatgcc aatctgcgcg ttcacacgcg cgcagaaatc gtcgaactgc atcgccgcgc cggtgtgccc accatctatg tgacccacga tcaggctgaa gcactcagca tggccgaccg cgttgcggtg atgatgggcg gcaaactgct ccagatcgac acgcctggcg cgatctataa cgagccgcgc catatcgagg tcgcgcaatt catcggcacg ccgcgcatca atattctgga agcattggtc agcgataacg gcgtggtgca ttttgtngcc gcgccatcct tcnggcgtcg ccgcccattc cggcagcata ttcgtattgg cattcgtcct gaacattttc agctcngncg cacgacagac nnnatgcaag caccgtttca cgatcgaatn nnnnncgaag tccttgccta tctcaagtcc ngnnnnnnng anctcacgnn agcttcacnn nannnnnnnn atntcntngg ctnctgnnnn tggcntttgn tncagcaagc tatnnnt
Expected Protein Sequence
MAHHHHHHMS TLVLRDIGKS YGGNRVLDGI SIEAAEGEFI ALVGPSGCGK STLLRILAGL DHADEGSVEL AGCNITGAHP ADRNIAMVFQ SYALYPHLTA AQNIAVPLAM RDLTVMERLP VIGAFLPGQR DKRAAIQKQV RATAASLKIA PLLDRKPGQM SGGQRQRVAL ARALVRQPGA FLMDEPLSNL DANLRVHTRA EIVELHRRAG VPTIYVTHDQ AEALSMADRV AVMMGGKLLQ IDTPGAIYNE PRHIEVAQFI GTPRINILEA LVSDNGVVHF VGRAILQGVA AHSGSNIRIG IRPEHFQLRP HDKTGIASTV SRIEFLGSEV LAYLKVPGAG QDLTVKLSPE DARDIVVGLP AGLHFDASKA YLFGEGGLRL RQPAGNIVKL ASPAKATAHV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgagca cactcgtcct tcgcgacatt ggcaaatcct acgggggaaa tcgcgttctc gatggcattt cgatcgaagc cgcagaaggc gaattcatcg ccctcgtcgg cccgtccggc tgcggcaaga gcacattgct gcgtattctt gccgggctcg atcatgcgga cgaaggaagc gtggagcttg ccgggtgcaa cataaccggc gcccaccccg ccgaccggaa cattgctatg gtgttccagt cctatgcact ctatccccat ctgacagcgg cgcagaacat cgccgtgccg ctggcgatgc gcgatctgac cgtaatggaa cgcctgccgg tgatcggcgc cttcctgccc ggccagcgcg acaagcgcgc cgccatccag aagcaggtca gggcaaccgc ggccagcctc aagatcgccc ccctgctcga ccgcaagccg ggccagatgt ccggtggcca gcgccagcgt gtggcattgg cccgcgcact tgtccgccag cccggcgcat ttctgatgga cgaaccgctg tccaatctcg atgccaatct gcgcgttcac acgcgcgcag aaatcgtcga actgcatcgc cgcgccggtg tgcccaccat ctatgtgacc cacgatcagg ctgaagcact cagcatggcc gaccgcgttg cggtgatgat gggcggcaaa ctgctccaga tcgacacgcc tggcgcgatc tataacgagc cgcgccatat cgaggtcgcg caattcatcg gcacgccgcg catcaatatt ctggaagcat tggtcagcga taacggcgtg gtgcattttg taggccgcgc catccttcag ggcgtcgccg cccattccgg cagcaatatt cgtattggca ttcgtcctga acattttcag ctcaggccgc acgacaagac aggcattgca agcaccgttt cacggatcga attcctcggc tcggaagtcc ttgcctatct caaggtcccc ggcgccgggc aggatctcac ggtgaagctt tcaccggaag acgcccgcga catcgtcgtc ggcctgcctg cgggtctgca ttttgatgcc agcaaggctt atctcttcgg ggaaggtggc ctgcgcctgc gccagcccgc cggaaatatc gtgaagcttg catccccggc aaaggctacc gctcatgtct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat