SSGCID: Target Page

BrabA.00057.k

Oligopeptide transport system permease protein OppD (TC 3.A.1.5.1)

CENTER ID:	BrabA.00057.k
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.00057.k.B1.GE37701	Full length( BrabA.00057.k )		1	251

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruMel86222_2751
BV-BRC:	fig\|359391.4.peg.2751
RefSeq:	YP_418648.1
UniProt:	Q2YL36

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSKLVRLEKL SVRYGREMAL QNIDLDIASG EILAVIGESG SGKSTLALAL ASLLPAGAHV TGGMEWAAGR LQPGRDIGFI FQDPASSFDP LMTVGAQLVE TIRAHENSGR GQARAKAIAL LERVHIPAPD ESFHRYPHQF SGGQKQRIAV ALAIAANSRL LIADEPTSAL DTIVQKEITA LLQRLVREDG MTLVFITHDI ALASGLADRI AVFRHGRLIE TGSAAFIVAK PQTDYTKALI AAVPDLEAVH G

NT Sequence

ATGAGCAAGC TCGTTCGTCT TGAAAAGCTT TCAGTGCGCT ATGGGCGTGA AATGGCCCTG CAAAACATTG ATCTCGATAT TGCTTCGGGC GAAATATTGG CCGTGATTGG TGAAAGCGGC TCCGGGAAAA GCACGTTGGC GCTGGCCCTT GCCTCGCTTC TGCCTGCTGG CGCGCATGTT ACCGGCGGCA TGGAATGGGC GGCAGGCCGC TTGCAGCCGG GGCGTGATAT CGGCTTCATA TTTCAGGATC CGGCATCAAG TTTTGATCCG CTGATGACGG TCGGGGCGCA GCTTGTCGAG ACGATCCGCG CTCATGAAAA CTCCGGCAGG GGGCAAGCGA GGGCAAAAGC TATCGCGCTT CTGGAACGTG TACATATTCC TGCACCGGAT GAGAGCTTTC ACCGCTATCC GCACCAGTTT TCCGGCGGGC AGAAGCAGCG CATCGCGGTT GCGCTCGCCA TCGCGGCAAA CTCGCGCCTT CTGATTGCCG ATGAGCCGAC GAGTGCGCTC GATACTATCG TGCAGAAGGA AATAACAGCC CTTTTGCAAA GGCTGGTGCG GGAAGACGGG ATGACGCTCG TTTTCATCAC GCATGATATT GCGCTTGCTT CGGGATTGGC GGATCGCATC GCGGTTTTTC GCCATGGGCG GCTTATCGAA ACGGGAAGTG CGGCTTTTAT TGTGGCCAAG CCGCAGACGG ATTACACGAA GGCGCTTATC GCCGCCGTAC CGGATTTGGA GGCGGTGCAT GGC

Details for BrabA.00057.k.B1.GE37701

HARVESTED ON:	2/5/2014
SEQUENCED ON:	2/6/2014
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	98
PERCENT COVERAGE:	101

Validated AA Sequence

MAHHHHHHMS KLVRLEKLSV RYGREMALQN IDLDIASGEI LAVIGESGSG KSTLALALAS LLPAGAHVTG GMEWAAGRLQ PGRDIGFIFQ DPASSFDPLM TVGAQLVETI RAHENSGRGQ ARAKAIALLE RVHIPAPDES FHRYPHQFSG GQKQRIAVAL AIAANSRLLI ADEPTSALDT IVQKEITALL QRLVREDGMT LVFITHDIAL ASGLADRIAV FRHGRLIETG SAAFIVAKPQ TDYTKALIAA VPDLGGGAWL SE

Validated NT Sequence

cnctagatat tttgtttnct ttaagaagga gatataccat ggctcaccac caccaccacc atatgagcaa gctcgttcgt cttgaaaagc tttcagtgcg ctatgggcgt gaaatggccc tgcaaaacat tgatctcgat attgcttcgg gcgaaatatt ggccgtgatt ggtgaaagcg gctccgggaa aagcacgttg gcgctggccc ttgcctcgct tctgcctgct ggcgcgcatg ttaccggcgg catggaatgg gcggcaggcc gcttgcagcc ggggcgtgat atcggcttca tatttcagga tccggcatca agttttgatc cgctgatgac ggtcggggcg cagcttgtcg agacgatccg cgctcatgaa aactccggca gggggcaagc gagggcaaaa gctatcgcgc ttctggaacg tgtacatatt cctgcaccgg atgagagctt tcaccgctat ccgcaccagt tttccggcgg gcagaagcag cgcatcgcgg ttgcgctcgc catcgcggca aactcgcgcc ttctgattgc cgatgagccg acgagtgcgc tcgatactat cgtgcagaag gaaataacag cccttttgca aaggctggtg cgggaagacg ggatgacgct cgttttcatc acgcatgata ttgcgcttgc ttcgggattg gcggatcgca tcgcggtttt tcgccatggg cggcttatcg aaacgggaag tgcggctttt attgtggcca agccgcagac ggattacacg aaggcgctta tcgccgccgt accggatttg ggaggcggtg catggctaag tgagtaagat anatcnncnc cnnncnnnnn nnnnnnnnaa anaaaanaat angnnanntg tccncncacc tcncgcntan ctcanggtgt catgcttgcc gcgagnccnc gatgacgtca ctncggatct gtancntatc ntccntcann caaagtctan accatcnnnn ntcgctgant nannntatng nnncngnnna cnntgnnnnn ggctaatctn nattnngncn tnnnnnngac anancnnnan nnantnntgn nganccnact ngnnnnnntn cngnnnnata annannnnnn nnnc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagca agctcgttcg tcttgaaaag ctttcagtgc gctatgggcg tgaaatggcc ctgcaaaaca ttgatctcga tattgcttcg ggcgaaatat tggccgtgat tggtgaaagc ggctccggga aaagcacgtt ggcgctggcc cttgcctcgc ttctgcctgc tggcgcgcat gttaccggcg gcatggaatg ggcggcaggc cgcttgcagc cggggcgtga tatcggcttc atatttcagg atccggcatc aagttttgat ccgctgatga cggtcggggc gcagcttgtc gagacgatcc gcgctcatga aaactccggc agggggcaag cgagggcaaa agctatcgcg cttctggaac gtgtacatat tcctgcaccg gatgagagct ttcaccgcta tccgcaccag ttttccggcg ggcagaagca gcgcatcgcg gttgcgctcg ccatcgcggc aaactcgcgc cttctgattg ccgatgagcc gacgagtgcg ctcgatacta tcgtgcagaa ggaaataaca gcccttttgc aaaggctggt gcgggaagac gggatgacgc tcgttttcat cacgcatgat attgcgcttg cttcgggatt ggcggatcgc atcgcggttt ttcgccatgg gcggcttatc gaaacgggaa gtgcggcttt tattgtggcc aagccgcaga cggattacac gaaggcgctt atcgccgccg taccggattt ggaggcggtg catggctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t