BrabA.00057.j

ABC transporter, ATP-binding protein

CENTER ID: BrabA.00057.j
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.00057.j.B1.GE37700 Full length( BrabA.00057.j ) 1 355
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruMel86222_2723
BV-BRC: fig|359391.4.peg.2723
RefSeq: YP_418625.1
UniProt: Q2YJ39

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQAASLPFNE IRLERMSRAF GSHNALKSIT LSISKGEFIA LLGPSGCGKS TALNCIAGLL ATTGGGIYID EKRIDTLKPE DRGFGMVFQN YALFPHMSVR ENVGFGLKMR GTPKSEIRKR VDEAIGMVRL NGQEEKLPGQ LSGGQQQRVA IARAIVIEPP LVLMDEPLSN LDAKLRLEMR AEIRRIHNQL GSTTIYVTHD QDEALSLADR IVVLRDGEIR QVGVPSDLYE RPDHLDVAEF MGYRNRLPGK VTAIDGERAT ISIGGSLIAG IARVPLAIGQ DVVLAARPDD VATGPASPAA LQAKVETIEY RGRDFVGTAN MSDGHNLVFH ASAHAELGSA VSLTVDPNRA LIFPA
NT Sequence
ATGCAAGCAG CATCTTTGCC CTTCAATGAA ATTCGGCTCG AAAGGATGAG CCGTGCTTTT GGGTCACATA ATGCACTCAA AAGCATTACG CTGTCGATCA GCAAGGGTGA GTTCATTGCA TTGCTGGGCC CTTCGGGCTG CGGAAAATCA ACGGCTTTGA ACTGTATTGC CGGTCTGCTG GCTACCACCG GTGGCGGCAT CTATATCGAT GAAAAGCGCA TCGATACATT GAAGCCGGAA GACCGTGGCT TTGGGATGGT GTTTCAGAAT TATGCGCTGT TTCCACATAT GAGCGTCCGC GAGAATGTGG GCTTCGGCCT GAAAATGCGT GGCACACCGA AGTCAGAAAT CCGAAAACGT GTAGATGAAG CCATAGGAAT GGTTCGTCTC AATGGTCAGG AAGAAAAACT GCCCGGCCAG CTTTCAGGCG GTCAGCAACA ACGGGTTGCA ATTGCCCGCG CCATCGTCAT CGAACCGCCA TTGGTTCTTA TGGATGAGCC GCTTTCCAAT CTCGATGCCA AACTGCGCCT GGAAATGCGG GCCGAAATTC GCCGCATTCA CAATCAGCTT GGCTCGACCA CGATTTATGT CACGCACGAT CAGGATGAGG CGCTCTCGCT TGCAGATCGT ATAGTGGTGC TGCGCGATGG CGAGATCCGC CAGGTTGGCG TGCCCAGCGA TCTTTATGAG CGTCCCGACC ATTTGGACGT CGCCGAATTT ATGGGATATC GCAATCGCCT CCCCGGTAAG GTGACGGCAA TTGATGGAGA GCGGGCAACT ATATCCATCG GCGGTTCGCT GATTGCAGGT ATTGCCCGCG TACCTCTTGC CATAGGTCAG GATGTCGTGC TTGCAGCGCG CCCCGATGAC GTCGCTACGG GACCTGCAAG CCCAGCGGCC CTTCAAGCAA AAGTCGAGAC GATCGAATAT CGCGGACGTG ATTTTGTGGG CACCGCGAAC ATGTCAGACG GGCATAATCT TGTGTTTCAC GCCTCCGCCC ACGCTGAACT AGGAAGTGCA GTTTCTCTGA CGGTCGACCC AAACCGTGCC CTGATTTTCC CGGCG
Details for BrabA.00057.j.B1.GE37700
HARVESTED ON: 2/5/2014
SEQUENCED ON: 2/6/2014
EXPECTED MW: 39kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 85
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMQ AASLPFNEIR LERMSRAFGS HNALKSITLS ISKGEFIALL GPSGCGKSTA LNCIAGLLAT TGGGIYIDEK RIDTLKPEDR GFGMVFQNYA LFPHMSVREN VGFGLKMRGT PKSEIRKRVD EAIGMVRLNG QEEKLPGQLS GGQQQRVAIA RAIVIEPPLV LMDEPLSNLD AKLRLEMRAE IRRIHNQLGS TTIYVTHDQD EALSLADRIV VLRDGEIRQV GVPSDLYERP DHLDVAEFMG YRNRLPGKVT AIDGERATIS IGGSLIAGIA RVPLAIGXMS CLQRAPMTSL RDLQAQRPFK QKSRRSNIAD VIXGHRXMSX GHILXTLRXA DXXCXXLXXT QRALIXRXXD
Validated NT Sequence
ggnnncncta gaatattttg tttaacttta agaaggagat ataccatggc tcaccaccac caccaccata tgcaagcagc atctttgccc ttcaatgaaa ttcggctcga aaggatgagc cgtgcttttg ggtcacataa tgcactcaaa agcattacgc tgtcgatcag caagggtgag ttcattgcat tgctgggccc ttcgggctgc ggaaaatcaa cggctttgaa ctgtattgcc ggtctgctgg ctaccaccgg tggcggcatc tatatcgatg aaaagcgcat cgatacattg aagccggaag accgtggctt tgggatggtg tttcagaatt atgcgctgtt tccacatatg agcgtccgcg agaatgtggg cttcggcctg aaaatgcgtg gcacaccgaa gtcagaaatc cgaaaacgtg tagatgaagc cataggaatg gttcgtctca atggtcagga agaaaaactg cccggccagc tttcaggcgg tcagcaacaa cgggttgcaa ttgcccgcgc catcgtcatc gaaccgccat tggttcttat ggatgagccg ctttccaatc tcgatgccaa actgcgcctg gaaatgcggg ccgaaattcg ccgcattcac aatcagcttg gctcgaccac gatttatgtc acgcacgatc aggatgaggc gctctcgctt gcagatcgta tagtggtgct gcgcgatggc gagatccgcc aggttggcgt gcccagcgat ctttatgagc gtcccgacca tttggacgtc gccgaattta tgggatatcg caatcgcctc cccggtaagg tgacggcaat tgatggagag cgggcaacta tatccatcgg cggttcgctg attgcaggta ttgcccgcgt acctcttgcc ataggtcnga tgtcgtgctt gcagcgcgcc ccgatgacgt cgctacggga cctgcaagcc cagcggccct tcaagcaaaa gtcgagacga tcgaatatcg cggacgtgat ttnngggcac cgcgnnatgt cngangggca tatcttgntn acgctccgcc ncgctgactn nnagtgcann nctctgangn cgacccaacg tgccctgatt tnccggcnta ntgata
Expected Protein Sequence
MAHHHHHHMQ AASLPFNEIR LERMSRAFGS HNALKSITLS ISKGEFIALL GPSGCGKSTA LNCIAGLLAT TGGGIYIDEK RIDTLKPEDR GFGMVFQNYA LFPHMSVREN VGFGLKMRGT PKSEIRKRVD EAIGMVRLNG QEEKLPGQLS GGQQQRVAIA RAIVIEPPLV LMDEPLSNLD AKLRLEMRAE IRRIHNQLGS TTIYVTHDQD EALSLADRIV VLRDGEIRQV GVPSDLYERP DHLDVAEFMG YRNRLPGKVT AIDGERATIS IGGSLIAGIA RVPLAIGQDV VLAARPDDVA TGPASPAALQ AKVETIEYRG RDFVGTANMS DGHNLVFHAS AHAELGSAVS LTVDPNRALI FPA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaag cagcatcttt gcccttcaat gaaattcggc tcgaaaggat gagccgtgct tttgggtcac ataatgcact caaaagcatt acgctgtcga tcagcaaggg tgagttcatt gcattgctgg gcccttcggg ctgcggaaaa tcaacggctt tgaactgtat tgccggtctg ctggctacca ccggtggcgg catctatatc gatgaaaagc gcatcgatac attgaagccg gaagaccgtg gctttgggat ggtgtttcag aattatgcgc tgtttccaca tatgagcgtc cgcgagaatg tgggcttcgg cctgaaaatg cgtggcacac cgaagtcaga aatccgaaaa cgtgtagatg aagccatagg aatggttcgt ctcaatggtc aggaagaaaa actgcccggc cagctttcag gcggtcagca acaacgggtt gcaattgccc gcgccatcgt catcgaaccg ccattggttc ttatggatga gccgctttcc aatctcgatg ccaaactgcg cctggaaatg cgggccgaaa ttcgccgcat tcacaatcag cttggctcga ccacgattta tgtcacgcac gatcaggatg aggcgctctc gcttgcagat cgtatagtgg tgctgcgcga tggcgagatc cgccaggttg gcgtgcccag cgatctttat gagcgtcccg accatttgga cgtcgccgaa tttatgggat atcgcaatcg cctccccggt aaggtgacgg caattgatgg agagcgggca actatatcca tcggcggttc gctgattgca ggtattgccc gcgtacctct tgccataggt caggatgtcg tgcttgcagc gcgccccgat gacgtcgcta cgggacctgc aagcccagcg gcccttcaag caaaagtcga gacgatcgaa tatcgcggac gtgattttgt gggcaccgcg aacatgtcag acgggcataa tcttgtgttt cacgcctccg cccacgctga actaggaagt gcagtttctc tgacggtcga cccaaaccgt gccctgattt tcccggcgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat