BrabA.00057.g

ATP/GTP-binding site motif A (P-loop):ABC transporter:AAA ATPase

CENTER ID: BrabA.00057.g
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.00057.g.B1.GE27579 Full length( BrabA.00057.g ) 1 244
BrabA.00057.g.B1.GE27152 Full length( BrabA.00057.g ) 1 244
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_130962
PATRIC ID: fig|359391.4.peg.3461
RefSeq: YP_419277.1
UniProt: Q2YJA7
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTALLEVRNL TKRFGGLTAV NNVSFTLNKG EILGFIGPNG AGKTTVVGLI SGSIYPSSGE VYFKGARIDT VPMYRRAHMG IGRTFQIMKP FPGLSVLDNV AVGAMFGRKE AHHGLTAARE QARANLDFVG LGNAIDKRAE ELGGSDRKRL ELAKALAMQL ELLLCDEVMA GLNLVELEEV IEVIRKVRDS GVTILVIEHV MKAIKSLSDR LMMLHHGEKI AEGSTDAVLS NAVVIEAYLG KKRA
NT Sequence
ATGACCGCCC TGCTGGAAGT CAGGAACCTT ACCAAACGCT TTGGGGGGCT GACAGCAGTC AACAATGTCT CTTTCACGTT GAACAAGGGA GAAATATTGG GCTTCATCGG GCCGAATGGC GCTGGCAAGA CAACAGTGGT GGGGCTCATC AGCGGTTCGA TCTATCCGTC ATCGGGCGAG GTCTATTTCA AGGGCGCGCG CATCGACACC GTGCCGATGT ACAGGCGCGC TCATATGGGA ATTGGCCGCA CCTTTCAGAT CATGAAGCCT TTTCCCGGCC TGTCCGTACT GGATAATGTC GCTGTCGGCG CCATGTTCGG ACGCAAGGAA GCCCATCATG GCCTCACCGC CGCGCGTGAG CAGGCGCGCG CCAATCTGGA CTTCGTGGGG CTCGGCAATG CCATCGACAA GCGCGCCGAA GAATTGGGAG GCTCGGATCG CAAGCGGCTG GAACTGGCCA AGGCGCTCGC CATGCAGCTC GAACTTCTGC TGTGTGATGA AGTCATGGCG GGGCTTAATC TGGTCGAGCT TGAGGAAGTC ATCGAAGTCA TCCGCAAGGT ACGCGATTCA GGCGTGACCA TTCTCGTGAT CGAACATGTC ATGAAAGCGA TCAAGAGCCT GTCCGACCGG TTAATGATGC TGCACCACGG CGAAAAAATA GCGGAAGGCA GTACCGATGC GGTCCTGTCC AACGCGGTCG TGATCGAAGC CTATCTGGGA AAGAAGAGAG CA
Details for BrabA.00057.g.B1.GE27579
HARVESTED ON: 8/17/2009
SEQUENCED ON: 9/29/2009
EXPECTED MW: 27kDa
OBSERVED MW: 25kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMT ALLEVRNLTK RFGGLTAVNN VSFTLNKGEI LGFIGPNGAG KTTVVGLISG SIYPSSGEVY FKGARIDTVP MYRRAHMGIG RTFQIMKPFP GLSVLDNVAV GAMFGRKEAH HGLTAAREQA RANLDFVGLG NAIDKRAEEL GGSDRKRLEL AKALAMQLEL LLCDEVMAGL NLVELEEVIE VIRKVRDSGV TILVIEHVMK AIKSLSDRLM MLHHGEKIAE GSTDAVLSNA VVIEAYLGKK RA
Validated NT Sequence
atggctcacc accaccacca ccatatgacc gccctgctgg aagtcaggaa ccttaccaaa cgctttgggg ggctgacagc agtcaacaat gtctctttca cgttgaacaa gggagaaata ttaggcttca tcgggccgaa tggcgctggc aagacaacag tggtggggct catcagcggt tcgatctatc cgtcatcggg cgaggtctat ttcaagggcg cgcgcatcga caccgtgccg atgtacaggc gcgctcatat gggaattggc cgcacctttc agatcatgaa gccttttccc ggcctgtccg tactggataa tgtcgctgtc ggcgccatgt tcggacgcaa ggaagcccat catggcctca ccgccgcgcg tgagcaggcg cgcgccaatc tggacttcgt ggggctcggc aatgccatcg acaagcgcgc cgaagaattg ggaggctcgg atcgcaagcg gctggaactg gccaaggcgc tcgccatgca gctcgaactt ctgctgtgtg atgaagtcat ggcggggctt aatctggtcg agcttgagga agtcatcgaa gtcatccgca aggtacgcga ttcaggcgtg accattctcg tgatcgaaca tgtcatgaaa gcgatcaaga gcctgtccga ccggttaatg atgctgcacc acggcgaaaa aatagcggaa ggcagtaccg atgcggtcct gtccaacgcg gtcgtgatcg aagcctatct gggaaagaag agagcataag tgagtaagat
Expected Protein Sequence
MAHHHHHHMT ALLEVRNLTK RFGGLTAVNN VSFTLNKGEI LGFIGPNGAG KTTVVGLISG SIYPSSGEVY FKGARIDTVP MYRRAHMGIG RTFQIMKPFP GLSVLDNVAV GAMFGRKEAH HGLTAAREQA RANLDFVGLG NAIDKRAEEL GGSDRKRLEL AKALAMQLEL LLCDEVMAGL NLVELEEVIE VIRKVRDSGV TILVIEHVMK AIKSLSDRLM MLHHGEKIAE GSTDAVLSNA VVIEAYLGKK RA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccg ccctgctgga agtcaggaac cttaccaaac gctttggggg gctgacagca gtcaacaatg tctctttcac gttgaacaag ggagaaatat tgggcttcat cgggccgaat ggcgctggca agacaacagt ggtggggctc atcagcggtt cgatctatcc gtcatcgggc gaggtctatt tcaagggcgc gcgcatcgac accgtgccga tgtacaggcg cgctcatatg ggaattggcc gcacctttca gatcatgaag ccttttcccg gcctgtccgt actggataat gtcgctgtcg gcgccatgtt cggacgcaag gaagcccatc atggcctcac cgccgcgcgt gagcaggcgc gcgccaatct ggacttcgtg gggctcggca atgccatcga caagcgcgcc gaagaattgg gaggctcgga tcgcaagcgg ctggaactgg ccaaggcgct cgccatgcag ctcgaacttc tgctgtgtga tgaagtcatg gcggggctta atctggtcga gcttgaggaa gtcatcgaag tcatccgcaa ggtacgcgat tcaggcgtga ccattctcgt gatcgaacat gtcatgaaag cgatcaagag cctgtccgac cggttaatga tgctgcacca cggcgaaaaa atagcggaag gcagtaccga tgcggtcctg tccaacgcgg tcgtgatcga agcctatctg ggaaagaaga gagcatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for BrabA.00057.g.B1.GE27152
HARVESTED ON: 7/20/2009
SEQUENCED ON: 7/28/2009
EXPECTED MW: 27kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMT ALLEVRNLTK RFGGLTAVNN VSFTLNKGEI LGFIGPNGAG KTTVVGLISG SIYPSSGEVY FKGARIDTVP MYRRAHMGIG RTFQIMKPFP GLSVLDNVAV GAMFGRKEAH HGLTAAREQA RANLDFVGLG NAIDKRAEEL GGSDRKRLEL AKALAMQLEL LLCDEVMAGL NLVELEEVIE VIRKVRDSGV TILVIEHVMK AIKSLSDRLM MLHHGEKIAE GSTDAVLSNA VVIEAYLGKK RA
Validated NT Sequence
atggctcacc accaccacca ccatatgacc gccctgctgg aagtcaggaa ccttaccaaa cgctttgggg ggctgacagc agtcaacaat gtctctttca cgttgaacaa gggagaaata ttaggcttca tcgggccgaa tggcgctggc aagacaacag tggtggggct catcagcggt tcgatctatc cgtcatcggg cgaggtctat ttcaagggcg cgcgcatcga caccgtgccg atgtacaggc gcgctcatat gggaattggc cgcacctttc agatcatgaa gccttttccc ggcctgtccg tactggataa tgtcgctgtc ggcgccatgt tcggacgcaa ggaagcccat catggcctca ccgccgcgcg tgagcaggcg cgcgccaatc tggacttcgt ggggctcggc aatgccatcg acaagcgcgc cgaagaattg ggaggctcgg atcgcaagcg gctggaactg gccaaggcgc tcgccatgca gctcgaactt ctgctgtgtg atgaagtcat ggcggggctt aatctggtcg agcttgagga agtcatcgaa gtcatccgca aggtacgcga ttcaggcgtg accattctcg tgatcgaaca tgtcatgaaa gcgatcaaga gcctgtccga ccggttaatg atgctgcacc acggcgaaaa aatagcggaa ggcagtaccg atgcggtcct gtccaacgcg gtcgtgatcg aagcctatct gggaaagaag agagcataag tgagtaagat
Expected Protein Sequence
MAHHHHHHMT ALLEVRNLTK RFGGLTAVNN VSFTLNKGEI LGFIGPNGAG KTTVVGLISG SIYPSSGEVY FKGARIDTVP MYRRAHMGIG RTFQIMKPFP GLSVLDNVAV GAMFGRKEAH HGLTAAREQA RANLDFVGLG NAIDKRAEEL GGSDRKRLEL AKALAMQLEL LLCDEVMAGL NLVELEEVIE VIRKVRDSGV TILVIEHVMK AIKSLSDRLM MLHHGEKIAE GSTDAVLSNA VVIEAYLGKK RA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccg ccctgctgga agtcaggaac cttaccaaac gctttggggg gctgacagca gtcaacaatg tctctttcac gttgaacaag ggagaaatat tgggcttcat cgggccgaat ggcgctggca agacaacagt ggtggggctc atcagcggtt cgatctatcc gtcatcgggc gaggtctatt tcaagggcgc gcgcatcgac accgtgccga tgtacaggcg cgctcatatg ggaattggcc gcacctttca gatcatgaag ccttttcccg gcctgtccgt actggataat gtcgctgtcg gcgccatgtt cggacgcaag gaagcccatc atggcctcac cgccgcgcgt gagcaggcgc gcgccaatct ggacttcgtg gggctcggca atgccatcga caagcgcgcc gaagaattgg gaggctcgga tcgcaagcgg ctggaactgg ccaaggcgct cgccatgcag ctcgaacttc tgctgtgtga tgaagtcatg gcggggctta atctggtcga gcttgaggaa gtcatcgaag tcatccgcaa ggtacgcgat tcaggcgtga ccattctcgt gatcgaacat gtcatgaaag cgatcaagag cctgtccgac cggttaatga tgctgcacca cggcgaaaaa atagcggaag gcagtaccga tgcggtcctg tccaacgcgg tcgtgatcga agcctatctg ggaaagaaga gagcatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat