BrabA.00053.a

CbxX/CfqX superfamily:ATP/GTP-binding site motif A (P-loop):AAA ATPase:Heat shock protein HslVU

CENTER ID: BrabA.00053.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: in PDB
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BrabA.00053.a.A1.GE25382 Full length( BrabA.00053.a ) 1 434
BrabA.00053.a.A2.GE28035 Created from Decode construct for feature BrabA.00053.a 1 418
BrabA.00053.a.A3.GE28036 Created from Decode construct for feature BrabA.00053.a 1 427
BrabA.00053.a.A4.GE28037 Created from Decode construct for feature BrabA.00053.a 3 434
BrabA.00053.a.A5.GE28038 Created from Decode construct for feature BrabA.00053.a 3 418
BrabA.00053.a.A6.GE28039 Created from Decode construct for feature BrabA.00053.a 3 427
BrabA.00053.a.AE2.GE43126 Created from Decode construct for feature BrabA.00053.a 1 418
BrabA.00053.a.AE5.GE43127 Created from Decode construct for feature BrabA.00053.a 3 418
BrabA.00053.a.B5.GE43036 Created from Decode construct for feature BrabA.00053.a 3 418
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BrabA.00053.a.A1.PS00057 Full length( BrabA.00053.a ) 1 434
BrabA.00053.a.A5.PS00515 Created from Decode construct for feature BrabA.00053.a 3 418
BrabA.00053.a.A4.PS00518 Created from Decode construct for feature BrabA.00053.a 3 434
BrabA.00053.a.A3.PS00521 Created from Decode construct for feature BrabA.00053.a 1 427
BrabA.00053.a.A6.PS00531 Created from Decode construct for feature BrabA.00053.a 3 427
BrabA.00053.a.A2.PS00548 Created from Decode construct for feature BrabA.00053.a 1 418
Structures
7MHB
DEPOSITED: 4/15/2021
DETERMINATION: XRay
CLONE: BrabA.00053.a.AE5.GE43127
PROTEIN: BrabA.00053.a.AE5.PW38673
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_129971
PATRIC ID: fig|359391.4.peg.2164
RefSeq: YP_415408
UniProt: Q2YQZ4
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSNFSPREIV SELDRFIIGQ KDAKRAVAIA LRNRWRRQQL EGQMREEVMP KNILMIGPTG VGKTEISRRL AKLAGAPFVK VEATKFTEVG YVGRDVEQII RDLVEIAITL VREKRREDVK AKAHLNAEER VLDALVGKTA SPATRDSFRK KLRNGEMDDK EIEIEVSDSG ASPNFEIPGM PGANIGVLNI SDMLGKAMGG RTKTRKTTVK DSYPILINDE SDKLLDQDQI VQEALRVSED EGIVFIDEID KIAAREGGSG AGVSREGVQR DLLPLVEGTT VATKYGPVKT DHILFITSGA FHVSKPSDLL PELQGRLPIR VELSALTRED FRRILTETEA SLIKQYIALM ETEEVKLEFS DDAIDALADI AVDLNATVEN IGARRLQTVI EKVLDEISFT APDKAGATFI IDAAYVKEKI GGLAKNTDLS RFIL
NT Sequence
ATGAGCAATT TTTCTCCCCG TGAAATCGTT TCGGAGCTTG ACCGCTTCAT CATCGGCCAG AAGGACGCCA AGCGCGCGGT GGCCATTGCG CTGCGCAATC GCTGGCGCCG CCAGCAGCTT GAAGGGCAGA TGCGCGAAGA GGTCATGCCG AAGAATATCC TGATGATCGG GCCGACCGGC GTGGGCAAAA CGGAAATCTC GCGCCGTCTG GCCAAGCTTG CAGGCGCGCC CTTCGTCAAG GTGGAGGCCA CCAAATTTAC CGAAGTGGGC TATGTCGGCC GCGATGTGGA GCAGATCATC CGCGATCTGG TCGAGATCGC CATCACGCTT GTGCGTGAAA AGCGCCGCGA GGACGTGAAG GCCAAGGCGC ATCTCAACGC CGAGGAACGG GTTCTGGACG CGCTCGTGGG CAAGACGGCC AGCCCCGCCA CCCGTGACAG CTTCCGCAAG AAGCTGCGCA ATGGCGAGAT GGACGACAAG GAAATCGAGA TTGAGGTTTC CGATTCCGGC GCCAGCCCGA ACTTCGAGAT TCCCGGCATG CCGGGCGCGA ATATCGGCGT GCTCAATATT TCCGACATGC TGGGCAAGGC CATGGGCGGG CGCACCAAGA CGCGCAAGAC CACGGTGAAG GATTCCTACC CCATCCTCAT CAATGACGAA TCCGACAAGC TGCTCGATCA GGACCAGATC GTGCAGGAGG CGCTGCGCGT GAGCGAGGAT GAAGGCATCG TCTTCATCGA TGAGATTGAC AAGATCGCCG CGCGCGAAGG CGGGAGCGGG GCAGGCGTCT CGCGTGAAGG CGTGCAGCGC GACCTGCTAC CGCTTGTCGA AGGCACAACG GTCGCGACCA AATACGGGCC GGTGAAGACG GATCACATTC TTTTCATCAC CTCAGGCGCG TTCCATGTCT CCAAGCCATC CGATCTTCTG CCGGAATTGC AGGGCCGCCT GCCCATCCGC GTGGAACTTT CCGCGCTGAC GCGGGAGGAT TTCCGCCGCA TTCTGACGGA AACCGAGGCA AGCCTCATCA AGCAGTATAT TGCGCTCATG GAAACGGAAG AGGTGAAGCT TGAATTCTCC GACGACGCGA TTGATGCGCT GGCCGATATC GCGGTCGATC TCAACGCGAC GGTGGAGAAT ATCGGCGCCC GCCGCCTGCA AACCGTGATA GAGAAGGTGC TGGACGAAAT TTCCTTCACC GCGCCGGACA AGGCCGGAGC AACCTTCATC ATCGACGCAG CCTATGTGAA GGAGAAGATC GGCGGCCTTG CCAAAAACAC CGATCTTTCG CGCTTCATTC TT
Details for BrabA.00053.a.A1.GE25382
HARVESTED ON: 6/30/2008
SEQUENCED ON: 6/16/2009
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSNFSPREI VSELDRFIIG QKDAKRAVAI ALRNRWRRQQ LEGQMREEVM PKNILMIGPT GVGKTEISRR LAKLAGAPFV KVEATKFTEV GYVGRDVEQI IRDLVEIAIT LVREKRREDV KAKAHLNAEE RVLDALVGKT ASPATRDSFR KKLRNGEMDD KEIEIEVSDS GASPNFEIPG MPGANIGVLN ISDMLGKAMG GRTKTRKTTV KDSYPILIND ESDKLLDQDQ IVQEALRVSE DEGIVFIDEI DKIAAREGGS GAGVSREGVQ RDLLPLVEGT TVATKYGPVK TDHILFITSG AFHVSKPSDL LPELQGRLPI RVELSALTRE DFRRILTETE ASLIKQYIAL METEEVKLEF SDDAIDALAD IAVDLNATVE NIGARRLQTV IEKVLDEISF TAPDKAGATF IIDAAYVKEK IGGLAKNTDL SRFIL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcaatttt tctccccgtg aaatcgtttc ggagcttgac cgcttcatca tcggccagaa ggacgccaag cgcgcggtgg ccattgcgct gcgcaatcgc tggcgccgcc agcagcttga agggcagatg cgcgaagagg tcatgccgaa gaatatcctg atgatcgggc cgaccggcgt gggcaaaacg gaaatctcgc gccgtctggc caagcttgca ggcgcgccct tcgtcaaggt ggaggccacc aaatttaccg aagtgggcta tgtcggccgc gatgtggagc agatcatccg cgatctggtc gagatcgcca tcacgcttgt gcgtgaaaag cgccgcgagg acgtgaaggc caaggcgcat ctcaacgccg aggaacgggt tctggacgcg ctcgtgggca agacggccag ccccgccacc cgtgacagct tccgcaagaa gctgcgcaat ggcgagatgg acgacaagga aatcgagatt gaggtttccg attccggcgc cagcccgaac ttcgagattc ccggcatgcc gggcgcgaat atcggcgtgc tcaatatttc cgacatgctg ggcaaggcca tgggcgggcg caccaagacg cgcaagacca cggtgaagga ttcctacccc atcctcatca atgacgaatc cgacaagctg ctcgatcagg accagatcgt gcaggaggcg ctgcgcgtga gcgaggatga aggcatcgtc ttcatcgatg agattgacaa gatcgccgcg cgcgaaggcg ggagcggggc aggcgtctcg cgtgaaggcg tgcagcgcga cctgctaccg cttgtcgaag gcacaacggt cgcgaccaaa tacgggccgg tgaagacgga tcacattctt ttcatcacct caggcgcgtt ccatgtctcc aagccatccg atcttctgcc ggaattgcag ggccgcctgc ccatccgcgt ggaactttcc gcgctgacgc gggaggattt ccgccgcatt ctgacggaaa ccgaggcaag cctcatcaag cagtatattg cgctcatgga aacggaagag gtgaagcttg aattctccga cgacgcgatt gatgcgctgg ccgatatcgc ggtcgatctc aacgcgacgg tggagaatat cggcgcccgc cgcctgcaaa ccgtgataga gaaggtgctg gacgaaattt ccttcaccgc gccggacaag gccggagcaa ccttcatcat cgacgcagcc tatgtgaagg agaagatcgg cggccttgcc aaaaacaccg atctttcgcg cttcattctt taaaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for BrabA.00053.a.A2.GE28035
HARVESTED ON: 12/7/2009
SEQUENCED ON: 12/23/2009
EXPECTED MW: 48kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSNFSPREI VSELDRFIIG QKDAKRAVAI ALRNRWRRQQ LEGQMREEVM PKNILMIGPT GVGKTEISRR LAKLAGAPFV KVEATKFTEV GYVGRDVEQI IRDLVEIAIT LVREKRREDV KAKAHLNAEE RVLDALVGKT ASPATRDSFR KKLRNGEMDD KEIEIEVSDS GASPNFEIPG MPGANIGVLN ISDMLGKAMG GRTKTRKTTV KDSYPILIND ESDKLLDQDQ IVQEALRVSE DEGIVFIDEI DKIAAREGGS GAGVSREGVQ RDLLPLVEGT TVATKYGPVK TDHILFITSG AFHVSKPSDL LPELQGRLPI RVELSALTRE DFRRILTETE ASLIKQYIAL METEEVKLEF SDDAIDALAD IAVDLNATVE NIGARRLQTV IEKVLDEISF TAPDKAGATF IIDAAYVKE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcaatttt tctccccgtg aaatcgtttc ggagcttgac cgcttcatca tcggccagaa ggacgccaag cgcgcggtgg ccattgcgct gcgcaatcgc tggcgccgcc agcagcttga agggcagatg cgcgaagagg tcatgccgaa gaatatcctg atgatcgggc cgaccggcgt gggcaaaacg gaaatctcgc gccgtctggc caagcttgca ggcgcgccct tcgtcaaggt ggaggccacc aaatttaccg aagtgggcta tgtcggccgc gatgtggagc agatcatccg cgatctggtc gagatcgcca tcacgcttgt gcgtgaaaag cgccgcgagg acgtgaaggc caaggcgcat ctcaacgccg aggaacgggt tctggacgcg ctcgtgggca agacggccag ccccgccacc cgtgacagct tccgcaagaa gctgcgcaat ggcgagatgg acgacaagga aatcgagatt gaggtttccg attccggcgc cagcccgaac ttcgagattc ccggcatgcc gggcgcgaat atcggcgtgc tcaatatttc cgacatgctg ggcaaggcca tgggcgggcg caccaagacg cgcaagacca cggtgaagga ttcctacccc atcctcatca atgacgaatc cgacaagctg ctcgatcagg accagatcgt gcaggaggcg ctgcgcgtga gcgaggatga aggcatcgtc ttcatcgatg agattgacaa gatcgccgcg cgcgaaggcg ggagcggggc aggcgtctcg cgtgaaggcg tgcagcgcga cctgctaccg cttgtcgaag gcacaacggt cgcgaccaaa tacgggccgg tgaagacgga tcacattctt ttcatcacct caggcgcgtt ccatgtctcc aagccatccg atcttctgcc ggaattgcag ggccgcctgc ccatccgcgt ggaactttcc gcgctgacgc gggaggattt ccgccgcatt ctgacggaaa ccgaggcaag cctcatcaag cagtatattg cgctcatgga aacggaagag gtgaagcttg aattctccga cgacgcgatt gatgcgctgg ccgatatcgc ggtcgatctc aacgcgacgg tggagaatat cggcgcccgc cgcctgcaaa ccgtgataga gaaggtgctg gacgaaattt ccttcaccgc gccggacaag gccggagcaa ccttcatcat cgacgcagcc tatgtgaagg agaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for BrabA.00053.a.A3.GE28036
HARVESTED ON: 12/7/2009
SEQUENCED ON: 12/23/2009
EXPECTED MW: 49kDa
OBSERVED MW: 60kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 93
PERCENT COVERAGE: 66
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSNFSPREI VSELDRFIIG QKDAKRAVAI ALRNRWRRQQ LEGQMREEVM PKNILMIGPT GVGKTEISRR LAKLAGAPFV KVEATKFTEV GYVGRDVEQI IRDLVEIAIT LVREKRREDV KAKAHLNAEE RVLDALVGKT ASPATRDSFR KKLRNGEMDD KEIEIEVSDS GASPNFEIPG MPGANIGVLN ISDMLGKAMG GRTKTRKTTV KDSYPILIND ESDXLLDQDQ IVXXALRVSE DEGIVFIDEX DKIAAREGGS GAXSRVXAXS ATCYRLS
Validated NT Sequence
ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcaatttt tctccccgtg aaatcgtttc ggagcttgac cgcttcatca tcggccagaa ggacgccaag cgcgcggtgg ccattgcgct gcgcaatcgc tggcgccgcc agcagcttga agggcagatg cgcgaagagg tcatgccgaa gaatatcctg atgatcgggc cgaccggcgt gggcaaaacg gaaatctcgc gccgtctggc caagcttgca ggcgcgccct tcgtcaaggt ggaggccacc aaatttaccg aagtgggcta tgtcggccgc gatgtggagc agatcatccg cgatctggtc gagatcgcca tcacgcttgt gcgtgaaaag cgccgcgagg acgtgaaggc caaggcgcat ctcaacgccg aggaacgggt tctggacgcg ctcgtgggca agacggccag ccccgccacc cgtgacagct tccgcaagaa gctgcgcaat ggcgagatgg acgacaagga aatcgagatt gaggtttccg attccggcgc cagcccgaac ttcgagattc ccggcatgcc gggcgcgaat atcggcgtgc tcaatatttc cgacatgctg ggcaaggcca tgggcgggcg caccaagacg cgcaagacca cggtgaagga ttcctacccc atcctcatca atgacgaatc cgacnagctg ctcgatcagg accagatcgt gcangangcg ctgcgcgtga gcgaggatga aggcatcgtc ttcatcgatg agantgacaa gatcgccgcg cgcgaaggcg ggagcggggc nnngtctcgc gtgaangcgt gnagcgcgac ctgctaccgc ttgtcna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSNFSPREI VSELDRFIIG QKDAKRAVAI ALRNRWRRQQ LEGQMREEVM PKNILMIGPT GVGKTEISRR LAKLAGAPFV KVEATKFTEV GYVGRDVEQI IRDLVEIAIT LVREKRREDV KAKAHLNAEE RVLDALVGKT ASPATRDSFR KKLRNGEMDD KEIEIEVSDS GASPNFEIPG MPGANIGVLN ISDMLGKAMG GRTKTRKTTV KDSYPILIND ESDKLLDQDQ IVQEALRVSE DEGIVFIDEI DKIAAREGGS GAGVSREGVQ RDLLPLVEGT TVATKYGPVK TDHILFITSG AFHVSKPSDL LPELQGRLPI RVELSALTRE DFRRILTETE ASLIKQYIAL METEEVKLEF SDDAIDALAD IAVDLNATVE NIGARRLQTV IEKVLDEISF TAPDKAGATF IIDAAYVKEK IGGLAKNT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcaatttt tctccccgtg aaatcgtttc ggagcttgac cgcttcatca tcggccagaa ggacgccaag cgcgcggtgg ccattgcgct gcgcaatcgc tggcgccgcc agcagcttga agggcagatg cgcgaagagg tcatgccgaa gaatatcctg atgatcgggc cgaccggcgt gggcaaaacg gaaatctcgc gccgtctggc caagcttgca ggcgcgccct tcgtcaaggt ggaggccacc aaatttaccg aagtgggcta tgtcggccgc gatgtggagc agatcatccg cgatctggtc gagatcgcca tcacgcttgt gcgtgaaaag cgccgcgagg acgtgaaggc caaggcgcat ctcaacgccg aggaacgggt tctggacgcg ctcgtgggca agacggccag ccccgccacc cgtgacagct tccgcaagaa gctgcgcaat ggcgagatgg acgacaagga aatcgagatt gaggtttccg attccggcgc cagcccgaac ttcgagattc ccggcatgcc gggcgcgaat atcggcgtgc tcaatatttc cgacatgctg ggcaaggcca tgggcgggcg caccaagacg cgcaagacca cggtgaagga ttcctacccc atcctcatca atgacgaatc cgacaagctg ctcgatcagg accagatcgt gcaggaggcg ctgcgcgtga gcgaggatga aggcatcgtc ttcatcgatg agattgacaa gatcgccgcg cgcgaaggcg ggagcggggc aggcgtctcg cgtgaaggcg tgcagcgcga cctgctaccg cttgtcgaag gcacaacggt cgcgaccaaa tacgggccgg tgaagacgga tcacattctt ttcatcacct caggcgcgtt ccatgtctcc aagccatccg atcttctgcc ggaattgcag ggccgcctgc ccatccgcgt ggaactttcc gcgctgacgc gggaggattt ccgccgcatt ctgacggaaa ccgaggcaag cctcatcaag cagtatattg cgctcatgga aacggaagag gtgaagcttg aattctccga cgacgcgatt gatgcgctgg ccgatatcgc ggtcgatctc aacgcgacgg tggagaatat cggcgcccgc cgcctgcaaa ccgtgataga gaaggtgctg gacgaaattt ccttcaccgc gccggacaag gccggagcaa ccttcatcat cgacgcagcc tatgtgaagg agaagatcgg cggccttgcc aaaaacacca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for BrabA.00053.a.A4.GE28037
HARVESTED ON: 12/7/2009
SEQUENCED ON: 12/23/2009
EXPECTED MW: 50kDa
OBSERVED MW: 60kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNFSPREIV SELDRFIIGQ KDAKRAVAIA LRNRWRRQQL EGQMREEVMP KNILMIGPTG VGKTEISRRL AKLAGAPFVK VEATKFTEVG YVGRDVEQII RDLVEIAITL VREKRREDVK AKAHLNAEER VLDALVGKTA SPATRDSFRK KLRNGEMDDK EIEIEVSDSG ASPNFEIPGM PGANIGVLNI SDMLGKAMGG RTKTRKTTVK DSYPILINDE SDKLLDQDQI VQEALRVSED EGIVFIDEID KIAAREGGSG AGVSREGVQR DLLPLVEGTT VATKYGPVKT DHILFITSGA FHVSKPSDLL PELQGRLPIR VELSALTRED FRRILTETEA SLIKQYIALM ETEEVKLEFS DDAIDALADI AVDLNATVEN IGARRLQTVI EKVLDEISFT APDKAGATFI IDAAYVKEKI GGLAKNTDLS RFIL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaatttttct ccccgtgaaa tcgtttcgga gcttgaccgc ttcatcatcg gccagaagga cgccaagcgc gcggtggcca ttgcgctgcg caatcgctgg cgccgccagc agcttgaagg gcagatgcgc gaagaggtca tgccgaagaa tatcctgatg atcgggccga ccggcgtggg caaaacggaa atctcgcgcc gtctggccaa gcttgcaggc gcgcccttcg tcaaggtgga ggccaccaaa tttaccgaag tgggctatgt cggccgcgat gtggagcaga tcatccgcga tctggtcgag atcgccatca cgcttgtgcg tgaaaagcgc cgcgaggacg tgaaggccaa ggcgcatctc aacgccgagg aacgggttct ggacgcgctc gtgggcaaga cggccagccc cgccacccgt gacagcttcc gcaagaagct gcgcaatggc gagatggacg acaaggaaat cgagattgag gtttccgatt ccggcgccag cccgaacttc gagattcccg gcatgccggg cgcgaatatc ggcgtgctca atatttccga catgctgggc aaggccatgg gcgggcgcac caagacgcgc aagaccacgg tgaaggattc ctaccccatc ctcatcaatg acgaatccga caagctgctc gatcaggacc agatcgtgca ggaggcgctg cgcgtgagcg aggatgaagg catcgtcttc atcgatgaga ttgacaagat cgccgcgcgc gaaggcggga gcggggcagg cgtctcgcgt gaaggcgtgc agcgcgacct gctaccgctt gtcgaaggca caacggtcgc gaccaaatac gggccggtga agacggatca cattcttttc atcacctcag gcgcgttcca tgtctccaag ccatccgatc ttctgccgga attgcagggc cgcctgccca tccgcgtgga actttccgcg ctgacgcggg aggatttccg ccgcattctg acggaaaccg aggcaagcct catcaagcag tatattgcgc tcatggaaac ggaagaggtg aagcttgaat tctccgacga cgcgattgat gcgctggccg atatcgcggt cgatctcaac gcgacggtgg agaatatcgg cgcccgccgc ctgcaaaccg tgatagagaa ggtgctggac gaaatttcct tcaccgcgcc ggacaaggcc ggagcaacct tcatcatcga cgcagcctat gtgaaggaga agatcggcgg ccttgccaaa aacaccgatc tttcgcgctt cattcttaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for BrabA.00053.a.A5.GE28038
HARVESTED ON: 12/7/2009
SEQUENCED ON: 12/23/2009
EXPECTED MW: 48kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 59
Validated AA Sequence
MDDKEIEIEV SDSGASPNFE IPGMPGANIG VLNISDMLGK AMGGRTKTRK TTVKDSYPIL INDESDKLLD QDQIVQEALR VSEDEGIVFI DEIDKIAARE GGSGAGVSRE GVQRDLLPLV EGTTVATKYG PVKTDHILFI TSGAFHVSKP SDLLPELQGR LPIRVELSAL TREDFRRILT ETEASLIKQY IALMETEEVK LEFSDDAIDA LADIAVDLNA TVENIGARRL QTVIEKVLDE ISFTAPDKAG ATFIIDAAYV
Validated NT Sequence
tcacataggc tgcgtcgatg atgaaggttg ctccggcctt gtccggcgcg gtgaaggaaa tttcgtccag caccttctct atcacggttt gcaggcggcg ggcgccgata ttctccaccg tcgcgttgag atcgaccgcg atatcggcca gcgcatcaat cgcgtcgtcg gagaattcaa gcttcacctc ttccgtttcc atgagcgcaa tatactgctt gatgaggctt gcctcggttt ccgtcagaat gcggcggaaa tcctcccgcg tcagcgcgga aagttccacg cggatgggca ggcggccctg caattccggc agaagatcgg atggcttgga gacatggaac gcgcctgagg tgatgaaaag aatgtgatcc gtcttcaccg gcccgtattt ggtcgcgacc gttgtgcctt cgacaagcgg tagcaggtcg cgctgcacgc cttcacgcga gacgcctgcc ccgctcccgc cttcgcgcgc ggcgatcttg tcaatctcat cgatgaagac gatgccttca tcctcgctca cgcgcagcgc ctcctgcacg atctggtcct gatcgagcag cttgtcggat tcgtcattga tgaggatggg gtaggaatcc ttcaccgtgg tcttgcgcgt cttggtgcgc ccgcccatgg ccttgcccag catgtcggaa atattgagca cgccgatatt cgcgcccggc atgccgggaa tctcgaagtt cgggctggcg ccggaatcgg aaacctcaat ctcgatttcc ttgtcgtcca tctcgccatt gcgcagcttc ttgcggaagc tgtcacgggt ggcggggctg gncngtcttg nccacgancg cgtccagaac ccgttcctcg gcgttgagan gcgccttgnn nttcacgtcc ncncggcgct tttcacgcac aagcgtgatg gc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNFSPREIV SELDRFIIGQ KDAKRAVAIA LRNRWRRQQL EGQMREEVMP KNILMIGPTG VGKTEISRRL AKLAGAPFVK VEATKFTEVG YVGRDVEQII RDLVEIAITL VREKRREDVK AKAHLNAEER VLDALVGKTA SPATRDSFRK KLRNGEMDDK EIEIEVSDSG ASPNFEIPGM PGANIGVLNI SDMLGKAMGG RTKTRKTTVK DSYPILINDE SDKLLDQDQI VQEALRVSED EGIVFIDEID KIAAREGGSG AGVSREGVQR DLLPLVEGTT VATKYGPVKT DHILFITSGA FHVSKPSDLL PELQGRLPIR VELSALTRED FRRILTETEA SLIKQYIALM ETEEVKLEFS DDAIDALADI AVDLNATVEN IGARRLQTVI EKVLDEISFT APDKAGATFI IDAAYVKE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaatttttct ccccgtgaaa tcgtttcgga gcttgaccgc ttcatcatcg gccagaagga cgccaagcgc gcggtggcca ttgcgctgcg caatcgctgg cgccgccagc agcttgaagg gcagatgcgc gaagaggtca tgccgaagaa tatcctgatg atcgggccga ccggcgtggg caaaacggaa atctcgcgcc gtctggccaa gcttgcaggc gcgcccttcg tcaaggtgga ggccaccaaa tttaccgaag tgggctatgt cggccgcgat gtggagcaga tcatccgcga tctggtcgag atcgccatca cgcttgtgcg tgaaaagcgc cgcgaggacg tgaaggccaa ggcgcatctc aacgccgagg aacgggttct ggacgcgctc gtgggcaaga cggccagccc cgccacccgt gacagcttcc gcaagaagct gcgcaatggc gagatggacg acaaggaaat cgagattgag gtttccgatt ccggcgccag cccgaacttc gagattcccg gcatgccggg cgcgaatatc ggcgtgctca atatttccga catgctgggc aaggccatgg gcgggcgcac caagacgcgc aagaccacgg tgaaggattc ctaccccatc ctcatcaatg acgaatccga caagctgctc gatcaggacc agatcgtgca ggaggcgctg cgcgtgagcg aggatgaagg catcgtcttc atcgatgaga ttgacaagat cgccgcgcgc gaaggcggga gcggggcagg cgtctcgcgt gaaggcgtgc agcgcgacct gctaccgctt gtcgaaggca caacggtcgc gaccaaatac gggccggtga agacggatca cattcttttc atcacctcag gcgcgttcca tgtctccaag ccatccgatc ttctgccgga attgcagggc cgcctgccca tccgcgtgga actttccgcg ctgacgcggg aggatttccg ccgcattctg acggaaaccg aggcaagcct catcaagcag tatattgcgc tcatggaaac ggaagaggtg aagcttgaat tctccgacga cgcgattgat gcgctggccg atatcgcggt cgatctcaac gcgacggtgg agaatatcgg cgcccgccgc ctgcaaaccg tgatagagaa ggtgctggac gaaatttcct tcaccgcgcc ggacaaggcc ggagcaacct tcatcatcga cgcagcctat gtgaaggaga aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for BrabA.00053.a.A6.GE28039
HARVESTED ON: 12/7/2009
SEQUENCED ON: 12/23/2009
EXPECTED MW: 49kDa
OBSERVED MW: 51kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MNFSPREIVS ELDRFIIGQK DAKRAVAIAL RNRWRRQQLE GQMREEVMPK NILMIGPTGV GKTEISRRLA KLAGAPFVKV EATKFTEVGY VGRDVEQIIR DLVEIAITLV REKRREDVKA KAHLNAEERV LDALVGKTAS PATRDSFRKK LRNGEMDDKE IEIEVSDSGA SPNFEIPGMP GANIGVLNIS DMLGKAMGGR TKTRKTTVKD SYPILINDES DKLLDQDQIV QEALRVSEDE GIVFIDEIDK IAAREGGSGA GVSREGVQRD LLPLVEGTTV ATKYGPVKTD HILFITSGAF HVSKPSDLLP ELQGRLPIRV ELSALTREDF RRILTETEAS LIKQYIALME TEEVKLEFSD DAIDALADIA VDLNATVENI GARRLQTVIE KVLDEISFTA PDKAGATFII DAAYVKEKIG GLAK
Validated NT Sequence
ttttggcaag gccgccgatc ttctccttca cataggctgc gtcgatgatg aaggttgctc cggccttgtc cggcgcggtg aaggaaattt cgtccagcac cttctctatc acggtttgca ggcggcgggc gccgatattc tccaccgtcg cgttgagatc gaccgcgata tcggccagcg catcaatcgc gtcgtcggag aattcaagct tcacctcttc cgtttccatg agcgcaatat actgcttgat gaggcttgcc tcggtttccg tcagaatgcg gcggaaatcc tcccgcgtca gcgcggaaag ttccacgcgg atgggcaggc ggccctgcaa ttccggcaga agatcggatg gcttggagac atggaacgcg cctgaggtga tgaaaagaat gtgatccgtc ttcaccggcc cgtatttggt cgcgaccgtt gtgccttcga caagcggtag caggtcgcgc tgcacgcctt cacgcgagac gcctgccccg ctcccgcctt cgcgcgcggc gatcttgtca atctcatcga tgaagacgat gccttcatcc tcgctcacgc gcagcgcctc ctgcacgatc tggtcctgat cgagcagctt gtcggattcg tcattgatga ggatggggta ggaatccttc accgtggtct tgcgcgtctt ggtgcgcccg cccatggcct tgcccagcat gtcggaaata ttgagcacgc cgatattcgc gcccggcatg ccgggaatct cgaagttcgg gctggcgccg gaatcggaaa cctcaatctc gatttccttg tcgtccatct cgccattgcg cagcttcttg cggaagctgt cacgggtggc ggggctggcc gtcttgccca cgagcgcgtc cagaacccgt tcctcggcgt tgagatgcgc cttggccttc acgtcctcgc ggcgcttttc acgcacaagc gtgatggcga tctcgaccag atcgcggatg atctgctcca catcgcggcc gacatagccc acttcggtaa atttggtggc ctccaccttg acgaagggcg cgcctgcaag cttggccaga cggcgcgaga tttccgtttt gcccacgccg gtcggcccga tcatcaggat attcttcggc atgacctctt cgcgcatctg cccttcaagc tgctggcggc gccagcgatt gcgcagcgca atggccaccg cgcgcttggc gtccttctgg ccgatgatga agcggtcaag ctccgaaacg atttcacggg gagaaaaatt catcgaacca ggaccctggg tctgagcttc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNFSPREIV SELDRFIIGQ KDAKRAVAIA LRNRWRRQQL EGQMREEVMP KNILMIGPTG VGKTEISRRL AKLAGAPFVK VEATKFTEVG YVGRDVEQII RDLVEIAITL VREKRREDVK AKAHLNAEER VLDALVGKTA SPATRDSFRK KLRNGEMDDK EIEIEVSDSG ASPNFEIPGM PGANIGVLNI SDMLGKAMGG RTKTRKTTVK DSYPILINDE SDKLLDQDQI VQEALRVSED EGIVFIDEID KIAAREGGSG AGVSREGVQR DLLPLVEGTT VATKYGPVKT DHILFITSGA FHVSKPSDLL PELQGRLPIR VELSALTRED FRRILTETEA SLIKQYIALM ETEEVKLEFS DDAIDALADI AVDLNATVEN IGARRLQTVI EKVLDEISFT APDKAGATFI IDAAYVKEKI GGLAKNT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaatttttct ccccgtgaaa tcgtttcgga gcttgaccgc ttcatcatcg gccagaagga cgccaagcgc gcggtggcca ttgcgctgcg caatcgctgg cgccgccagc agcttgaagg gcagatgcgc gaagaggtca tgccgaagaa tatcctgatg atcgggccga ccggcgtggg caaaacggaa atctcgcgcc gtctggccaa gcttgcaggc gcgcccttcg tcaaggtgga ggccaccaaa tttaccgaag tgggctatgt cggccgcgat gtggagcaga tcatccgcga tctggtcgag atcgccatca cgcttgtgcg tgaaaagcgc cgcgaggacg tgaaggccaa ggcgcatctc aacgccgagg aacgggttct ggacgcgctc gtgggcaaga cggccagccc cgccacccgt gacagcttcc gcaagaagct gcgcaatggc gagatggacg acaaggaaat cgagattgag gtttccgatt ccggcgccag cccgaacttc gagattcccg gcatgccggg cgcgaatatc ggcgtgctca atatttccga catgctgggc aaggccatgg gcgggcgcac caagacgcgc aagaccacgg tgaaggattc ctaccccatc ctcatcaatg acgaatccga caagctgctc gatcaggacc agatcgtgca ggaggcgctg cgcgtgagcg aggatgaagg catcgtcttc atcgatgaga ttgacaagat cgccgcgcgc gaaggcggga gcggggcagg cgtctcgcgt gaaggcgtgc agcgcgacct gctaccgctt gtcgaaggca caacggtcgc gaccaaatac gggccggtga agacggatca cattcttttc atcacctcag gcgcgttcca tgtctccaag ccatccgatc ttctgccgga attgcagggc cgcctgccca tccgcgtgga actttccgcg ctgacgcggg aggatttccg ccgcattctg acggaaaccg aggcaagcct catcaagcag tatattgcgc tcatggaaac ggaagaggtg aagcttgaat tctccgacga cgcgattgat gcgctggccg atatcgcggt cgatctcaac gcgacggtgg agaatatcgg cgcccgccgc ctgcaaaccg tgatagagaa ggtgctggac gaaatttcct tcaccgcgcc ggacaaggcc ggagcaacct tcatcatcga cgcagcctat gtgaaggaga agatcggcgg ccttgccaaa aacaccaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BrabA.00053.a.AE2.GE43126
HARVESTED ON: 5/17/2019
SEQUENCED ON: 5/24/2019
EXPECTED MW: 47kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MSNFSPREIV SELDRFIIGQ KDAKRAVAIA LRNRWRRQQL EGQMREEVMP KNILMIGPTG VGKTEISRRL AKLAGAPFVK VEATKFTEVG YVGRDVEQII RDLVEIAITL VREKRREDVK AKAHLNAEER VLDALVGKTA SPATRDSFRK KLRNGEMDDK EIEIEVSDSG ASPNFEIPGM PGANIGVLNI SDMLGKAMGG RTKTRKTTVK DSYPILINDE SDKLLDQDQI VQEALRVSED EGIVFIDEID KIAAREGGSG AGVSREGVQR DLLPLVEGTT VATKYGPVKT DHILFITSGA FHVSKPSDLL PELQGRLPIR VELSALTRED FRRILTETEA SLIKQYIALM ETEEVKLEFS DDAIDALADI AVDLNATVEN IGARRLQTVI EKVLDEISFT APDKAGATFI IDAAYVKEGH HHHHH
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccat gagcaatttt tctccccgtg aaatcgtttc ggagcttgac cgcttcatca tcggccagaa ggacgccaag cgcgcggtgg ccattgcgct gcgcaatcgc tggcgccgcc agcagcttga agggcagatg cgcgaagagg tcatgccgaa gaatatcctg atgatcgggc cgaccggcgt gggcaaaacg gaaatctcgc gccgtctggc caagcttgca ggcgcgccct tcgtcaaggt ggaggccacc aaatttaccg aagtgggcta tgtcggccgc gatgtggagc agatcatccg cgatctggtc gagatcgcca tcacgcttgt gcgtgaaaag cgccgcgagg acgtgaaggc caaggcgcat ctcaacgccg aggaacgggt tctggacgcg ctcgtgggca agacggccag ccccgccacc cgtgacagct tccgcaagaa gctgcgcaat ggcgagatgg acgacaagga aatcgagatt gaggtttccg attccggcgc cagcccgaac ttcgagattc ccggcatgcc gggcgcgaat atcggcgtgc tcaatatttc cgacatgctg ggcaaggcca tgggcgggcg caccaagacg cgcaagacca cggtgaagga ttcctacccc atcctcatca atgacgaatc cgacaagctg ctcgatcagg accagatcgt gcaggaggcg ctgcgcgtga gcgaggatga aggcatcgtc ttcatcgatg agattgacaa gatcgccgcg cgcgaaggcg ggagcggggc aggcgtctcg cgtgaaggcg tgcagcgcga cctgctaccg cttgtcgaag gcacaacggt cgcgaccaaa tacgggccgg tgaagacgga tcacattctt ttcatcacct caggcgcgtt ccatgtctcc aagccatccg atcttctgcc ggaattgcag ggccgcctgc ccatccgcgt ggaactttcc gcgctgacgc gggaggattt ccgccgcatt ctgacggaaa ccgaggcaag cctcatcaag cagtatattg cgctcatgga aacggaagag gtgaagcttg aattctccga cgacgcgatt gatgcgctgg ccgatatcgc ggtcgatctc aacgcgacgg tggagaatat cggcgcccgc cgcctgcaaa ccgtgataga gaaggtgctg gacgaaattt ccttcaccgc gccggacaag gccggagcaa ccttcatcat cgacgcagcc tatgtgaagg aggggcacca ccatcatcat cattaacgga tccgaattcg agctccgtcg acaagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat
Details for BrabA.00053.a.AE5.GE43127
HARVESTED ON: 5/17/2019
SEQUENCED ON: 5/24/2019
EXPECTED MW: 47kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: data unavailable
Validated AA Sequence
MNFSPREIVS ELDRFIIGQK DAKRAVAIAL RNRWRRQQLE GQMREEVMPK NILMIGPTGV GKTEISRRLA KLAGAPFVKV EATKFTEVGY VGRDVEQIIR DLVEIAITLV REKRREDVKA TAHLNAEERV LDALVGKTAS PATRDSFRKK LRNGEMDDKE IEIEVSDSGA SPNFEIPGMP GANIGVLNIS DMLGKAMGGR TKTRKTTVKD SYPILINDES DKLLDQDQIV QEALRVSEDE GIVFIDEIDK IAAREGGSGA GVSREGVQRD LLPLVEGTTV ATKYGPVKTD HILFITSGAF HVSKPSDLLP ELQGRLPIRV ELSALTREDF RRILTETEAS LIKQYIALME TEEVKLEFSD DAIDALADIA VDLNATVENI GARRLQTVIE KVLDEISFTA PDKAGATFII DAAYVKEAGH HHHHH
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
NFSPREIVSE LDRFIIGQKD AKRAVAIALR NRWRRQQLEG QMREEVMPKN ILMIGPTGVG KTEISRRLAK LAGAPFVKVE ATKFTEVGYV GRDVEQIIRD LVEIAITLVR EKRREDVKAK AHLNAEERVL DALVGKTASP ATRDSFRKKL RNGEMDDKEI EIEVSDSGAS PNFEIPGMPG ANIGVLNISD MLGKAMGGRT KTRKTTVKDS YPILINDESD KLLDQDQIVQ EALRVSEDEG IVFIDEIDKI AAREGGSGAG VSREGVQRDL LPLVEGTTVA TKYGPVKTDH ILFITSGAFH VSKPSDLLPE LQGRLPIRVE LSALTREDFR RILTETEASL IKQYIALMET EEVKLEFSDD AIDALADIAV DLNATVENIG ARRLQTVIEK VLDEISFTAP DKAGATFIID AAYVKEGHHH HHH
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccaa tttttctccc cgtgaaatcg tttcggagct tgaccgcttc atcatcggcc agaaggacgc caagcgcgcg gtggccattg cgctgcgcaa tcgctggcgc cgccagcagc ttgaagggca gatgcgcgaa gaggtcatgc cgaagaatat cctgatgatc gggccgaccg gcgtgggcaa aacggaaatc tcgcgccgtc tggccaagct tgcaggcgcg cccttcgtca aggtggaggc caccaaattt accgaagtgg gctatgtcgg ccgcgatgtg gagcagatca tccgcgatct ggtcgagatc gccatcacgc ttgtgcgtga aaagcgccgc gaggacgtga aggccaaggc gcatctcaac gccgaggaac gggttctgga cgcgctcgtg ggcaagacgg ccagccccgc cacccgtgac agcttccgca agaagctgcg caatggcgag atggacgaca aggaaatcga gattgaggtt tccgattccg gcgccagccc gaacttcgag attcccggca tgccgggcgc gaatatcggc gtgctcaata tttccgacat gctgggcaag gccatgggcg ggcgcaccaa gacgcgcaag accacggtga aggattccta ccccatcctc atcaatgacg aatccgacaa gctgctcgat caggaccaga tcgtgcagga ggcgctgcgc gtgagcgagg atgaaggcat cgtcttcatc gatgagattg acaagatcgc cgcgcgcgaa ggcgggagcg gggcaggcgt ctcgcgtgaa ggcgtgcagc gcgacctgct accgcttgtc gaaggcacaa cggtcgcgac caaatacggg ccggtgaaga cggatcacat tcttttcatc acctcaggcg cgttccatgt ctccaagcca tccgatcttc tgccggaatt gcagggccgc ctgcccatcc gcgtggaact ttccgcgctg acgcgggagg atttccgccg cattctgacg gaaaccgagg caagcctcat caagcagtat attgcgctca tggaaacgga agaggtgaag cttgaattct ccgacgacgc gattgatgcg ctggccgata tcgcggtcga tctcaacgcg acggtggaga atatcggcgc ccgccgcctg caaaccgtga tagagaaggt gctggacgaa atttccttca ccgcgccgga caaggccgga gcaaccttca tcatcgacgc agcctatgtg aaggaggggc accaccatca tcatcattaa cggatccgaa ttcgagctcc gtcgacaagc ttgcggccgc actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggat
Details for BrabA.00053.a.B5.GE43036
HARVESTED ON: 4/19/2019
SEQUENCED ON: 4/23/2019
EXPECTED MW: 47kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHNF SPREIVSELD RFIIGQKDAK RAVAIALRNR WRRQQLEGQM REEVMPKNIL MIGPTGVGKT EISRRLAKLA GAPFVKVEAT KFTEVGYVGR DVEQIIRDLV EIAITLVREK RREDVKAKAH LNAEERVLDA LVGKTASPAT RDSFRKKLRN GEMDDKEIEI EVSDSGASPN FEIPGMPGAN IGVLNISDML GKAMGGRTKT RKTTVKDSYP ILINDESDKL LDQDQIVQEA LRVSEDEGIV FIDEIDKIAA REGGSGAGVS REGVQRDLLP LVEGTTVATK YGPVKTDHIL FITSGAFHVS KPSDLLPELQ GRLPIRVELS ALTREDFRRI LTETEASLIK QYIALMETEE VKLEFSDDAI DALADIAVDL NATVENIGAR RLQTVIEKVL DEISFTAPDK AGATFIIDAA YVKE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataattttt ctccccgtga aatcgtttcg gagcttgacc gcttcatcat cggccagaag gacgccaagc gcgcggtggc cattgcgctg cgcaatcgct ggcgccgcca gcagcttgaa gggcagatgc gcgaagaggt catgccgaag aatatcctga tgatcgggcc gaccggcgtg ggcaaaacgg aaatctcgcg ccgtctggcc aagcttgcag gcgcgccctt cgtcaaggtg gaggccacca aatttaccga agtgggctat gtcggccgcg atgtggagca gatcatccgc gatctggtcg agatcgccat cacgcttgtg cgtgaaaagc gccgcgagga cgtgaaggcc aaggcgcatc tcaacgccga ggaacgggtt ctggacgcgc tcgtgggcaa gacggccagc cccgccaccc gtgacagctt ccgcaagaag ctgcgcaatg gcgagatgga cgacaaggaa atcgagattg aggtttccga ttccggcgcc agcccgaact tcgagattcc cggcatgccg ggcgcgaata tcggcgtgct caatatttcc gacatgctgg gcaaggccat gggcgggcgc accaagacgc gcaagaccac ggtgaaggat tcctacccca tcctcatcaa tgacgaatcc gacaagctgc tcgatcagga ccagatcgtg caggaggcgc tgcgcgtgag cgaggatgaa ggcatcgtct tcatcgatga gattgacaag atcgccgcgc gcgaaggcgg gagcggggca ggcgtctcgc gtgaaggcgt gcagcgcgac ctgctaccgc ttgtcgaagg cacaacggtc gcgaccaaat acgggccggt gaagacggat cacattcttt tcatcacctc aggcgcgttc catgtctcca agccatccga tcttctgccg gaattgcagg gccgcctgcc catccgcgtg gaactttccg cgctgacgcg ggaggatttc cgccgcattc tgacggaaac cgaggcaagc ctcatcaagc agtatattgc gctcatggaa acggaagagg tgaagcttga attctccgac gacgcgattg atgcgctggc cgatatcgcg gtcgatctca acgcgacggt ggagaatatc ggcgcccgcc gcctgcaaac cgtgatagag aaggtgctgg acgaaatttc cttcaccgcg ccggacaagg ccggagcaac cttcatcatc gacgcagcct atgtgaagga gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for BrabA.00053.a.A1.PS00057
PURIFICATION DATe: 8/6/2008
CONCENTRATION: 35.2mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 100µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSNFSPREI VSELDRFIIG QKDAKRAVAI ALRNRWRRQQ LEGQMREEVM PKNILMIGPT GVGKTEISRR LAKLAGAPFV KVEATKFTEV GYVGRDVEQI IRDLVEIAIT LVREKRREDV KAKAHLNAEE RVLDALVGKT ASPATRDSFR KKLRNGEMDD KEIEIEVSDS GASPNFEIPG MPGANIGVLN ISDMLGKAMG GRTKTRKTTV KDSYPILIND ESDKLLDQDQ IVQEALRVSE DEGIVFIDEI DKIAAREGGS GAGVSREGVQ RDLLPLVEGT TVATKYGPVK TDHILFITSG AFHVSKPSDL LPELQGRLPI RVELSALTRE DFRRILTETE ASLIKQYIAL METEEVKLEF SDDAIDALAD IAVDLNATVE NIGARRLQTV IEKVLDEISF TAPDKAGATF IIDAAYVKEK IGGLAKNTDL SRFIL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCAATTTT TCTCCCCGTG AAATCGTTTC GGAGCTTGAC CGCTTCATCA TCGGCCAGAA GGACGCCAAG CGCGCGGTGG CCATTGCGCT GCGCAATCGC TGGCGCCGCC AGCAGCTTGA AGGGCAGATG CGCGAAGAGG TCATGCCGAA GAATATCCTG ATGATCGGGC CGACCGGCGT GGGCAAAACG GAAATCTCGC GCCGTCTGGC CAAGCTTGCA GGCGCGCCCT TCGTCAAGGT GGAGGCCACC AAATTTACCG AAGTGGGCTA TGTCGGCCGC GATGTGGAGC AGATCATCCG CGATCTGGTC GAGATCGCCA TCACGCTTGT GCGTGAAAAG CGCCGCGAGG ACGTGAAGGC CAAGGCGCAT CTCAACGCCG AGGAACGGGT TCTGGACGCG CTCGTGGGCA AGACGGCCAG CCCCGCCACC CGTGACAGCT TCCGCAAGAA GCTGCGCAAT GGCGAGATGG ACGACAAGGA AATCGAGATT GAGGTTTCCG ATTCCGGCGC CAGCCCGAAC TTCGAGATTC CCGGCATGCC GGGCGCGAAT ATCGGCGTGC TCAATATTTC CGACATGCTG GGCAAGGCCA TGGGCGGGCG CACCAAGACG CGCAAGACCA CGGTGAAGGA TTCCTACCCC ATCCTCATCA ATGACGAATC CGACAAGCTG CTCGATCAGG ACCAGATCGT GCAGGAGGCG CTGCGCGTGA GCGAGGATGA AGGCATCGTC TTCATCGATG AGATTGACAA GATCGCCGCG CGCGAAGGCG GGAGCGGGGC AGGCGTCTCG CGTGAAGGCG TGCAGCGCGA CCTGCTACCG CTTGTCGAAG GCACAACGGT CGCGACCAAA TACGGGCCGG TGAAGACGGA TCACATTCTT TTCATCACCT CAGGCGCGTT CCATGTCTCC AAGCCATCCG ATCTTCTGCC GGAATTGCAG GGCCGCCTGC CCATCCGCGT GGAACTTTCC GCGCTGACGC GGGAGGATTT CCGCCGCATT CTGACGGAAA CCGAGGCAAG CCTCATCAAG CAGTATATTG CGCTCATGGA AACGGAAGAG GTGAAGCTTG AATTCTCCGA CGACGCGATT GATGCGCTGG CCGATATCGC GGTCGATCTC AACGCGACGG TGGAGAATAT CGGCGCCCGC CGCCTGCAAA CCGTGATAGA GAAGGTGCTG GACGAAATTT CCTTCACCGC GCCGGACAAG GCCGGAGCAA CCTTCATCAT CGACGCAGCC TATGTGAAGG AGAAGATCGG CGGCCTTGCC AAAAACACCG ATCTTTCGCG CTTCATTCTT TAAAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA
Details for BrabA.00053.a.A5.PS00515
PURIFICATION DATe: 3/15/2010
CONCENTRATION: 30.75mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 59
Protocol Notes
notes unavailable
Validated AA Sequence
MDDKEIEIEV SDSGASPNFE IPGMPGANIG VLNISDMLGK AMGGRTKTRK TTVKDSYPIL INDESDKLLD QDQIVQEALR VSEDEGIVFI DEIDKIAARE GGSGAGVSRE GVQRDLLPLV EGTTVATKYG PVKTDHILFI TSGAFHVSKP SDLLPELQGR LPIRVELSAL TREDFRRILT ETEASLIKQY IALMETEEVK LEFSDDAIDA LADIAVDLNA TVENIGARRL QTVIEKVLDE ISFTAPDKAG ATFIIDAAYV
Validated NT Sequence
tcacataggc tgcgtcgatg atgaaggttg ctccggcctt gtccggcgcg gtgaaggaaa tttcgtccag caccttctct atcacggttt gcaggcggcg ggcgccgata ttctccaccg tcgcgttgag atcgaccgcg atatcggcca gcgcatcaat cgcgtcgtcg gagaattcaa gcttcacctc ttccgtttcc atgagcgcaa tatactgctt gatgaggctt gcctcggttt ccgtcagaat gcggcggaaa tcctcccgcg tcagcgcgga aagttccacg cggatgggca ggcggccctg caattccggc agaagatcgg atggcttgga gacatggaac gcgcctgagg tgatgaaaag aatgtgatcc gtcttcaccg gcccgtattt ggtcgcgacc gttgtgcctt cgacaagcgg tagcaggtcg cgctgcacgc cttcacgcga gacgcctgcc ccgctcccgc cttcgcgcgc ggcgatcttg tcaatctcat cgatgaagac gatgccttca tcctcgctca cgcgcagcgc ctcctgcacg atctggtcct gatcgagcag cttgtcggat tcgtcattga tgaggatggg gtaggaatcc ttcaccgtgg tcttgcgcgt cttggtgcgc ccgcccatgg ccttgcccag catgtcggaa atattgagca cgccgatatt cgcgcccggc atgccgggaa tctcgaagtt cgggctggcg ccggaatcgg aaacctcaat ctcgatttcc ttgtcgtcca tctcgccatt gcgcagcttc ttgcggaagc tgtcacgggt ggcggggctg gncngtcttg nccacgancg cgtccagaac ccgttcctcg gcgttgagan gcgccttgnn nttcacgtcc ncncggcgct tttcacgcac aagcgtgatg gc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNFSPREIV SELDRFIIGQ KDAKRAVAIA LRNRWRRQQL EGQMREEVMP KNILMIGPTG VGKTEISRRL AKLAGAPFVK VEATKFTEVG YVGRDVEQII RDLVEIAITL VREKRREDVK AKAHLNAEER VLDALVGKTA SPATRDSFRK KLRNGEMDDK EIEIEVSDSG ASPNFEIPGM PGANIGVLNI SDMLGKAMGG RTKTRKTTVK DSYPILINDE SDKLLDQDQI VQEALRVSED EGIVFIDEID KIAAREGGSG AGVSREGVQR DLLPLVEGTT VATKYGPVKT DHILFITSGA FHVSKPSDLL PELQGRLPIR VELSALTRED FRRILTETEA SLIKQYIALM ETEEVKLEFS DDAIDALADI AVDLNATVEN IGARRLQTVI EKVLDEISFT APDKAGATFI IDAAYVKE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAATTTTTCT CCCCGTGAAA TCGTTTCGGA GCTTGACCGC TTCATCATCG GCCAGAAGGA CGCCAAGCGC GCGGTGGCCA TTGCGCTGCG CAATCGCTGG CGCCGCCAGC AGCTTGAAGG GCAGATGCGC GAAGAGGTCA TGCCGAAGAA TATCCTGATG ATCGGGCCGA CCGGCGTGGG CAAAACGGAA ATCTCGCGCC GTCTGGCCAA GCTTGCAGGC GCGCCCTTCG TCAAGGTGGA GGCCACCAAA TTTACCGAAG TGGGCTATGT CGGCCGCGAT GTGGAGCAGA TCATCCGCGA TCTGGTCGAG ATCGCCATCA CGCTTGTGCG TGAAAAGCGC CGCGAGGACG TGAAGGCCAA GGCGCATCTC AACGCCGAGG AACGGGTTCT GGACGCGCTC GTGGGCAAGA CGGCCAGCCC CGCCACCCGT GACAGCTTCC GCAAGAAGCT GCGCAATGGC GAGATGGACG ACAAGGAAAT CGAGATTGAG GTTTCCGATT CCGGCGCCAG CCCGAACTTC GAGATTCCCG GCATGCCGGG CGCGAATATC GGCGTGCTCA ATATTTCCGA CATGCTGGGC AAGGCCATGG GCGGGCGCAC CAAGACGCGC AAGACCACGG TGAAGGATTC CTACCCCATC CTCATCAATG ACGAATCCGA CAAGCTGCTC GATCAGGACC AGATCGTGCA GGAGGCGCTG CGCGTGAGCG AGGATGAAGG CATCGTCTTC ATCGATGAGA TTGACAAGAT CGCCGCGCGC GAAGGCGGGA GCGGGGCAGG CGTCTCGCGT GAAGGCGTGC AGCGCGACCT GCTACCGCTT GTCGAAGGCA CAACGGTCGC GACCAAATAC GGGCCGGTGA AGACGGATCA CATTCTTTTC ATCACCTCAG GCGCGTTCCA TGTCTCCAAG CCATCCGATC TTCTGCCGGA ATTGCAGGGC CGCCTGCCCA TCCGCGTGGA ACTTTCCGCG CTGACGCGGG AGGATTTCCG CCGCATTCTG ACGGAAACCG AGGCAAGCCT CATCAAGCAG TATATTGCGC TCATGGAAAC GGAAGAGGTG AAGCTTGAAT TCTCCGACGA CGCGATTGAT GCGCTGGCCG ATATCGCGGT CGATCTCAAC GCGACGGTGG AGAATATCGG CGCCCGCCGC CTGCAAACCG TGATAGAGAA GGTGCTGGAC GAAATTTCCT TCACCGCGCC GGACAAGGCC GGAGCAACCT TCATCATCGA CGCAGCCTAT GTGAAGGAGA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A
Details for BrabA.00053.a.A4.PS00518
PURIFICATION DATe: 3/12/2010
CONCENTRATION: 21.76mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNFSPREIV SELDRFIIGQ KDAKRAVAIA LRNRWRRQQL EGQMREEVMP KNILMIGPTG VGKTEISRRL AKLAGAPFVK VEATKFTEVG YVGRDVEQII RDLVEIAITL VREKRREDVK AKAHLNAEER VLDALVGKTA SPATRDSFRK KLRNGEMDDK EIEIEVSDSG ASPNFEIPGM PGANIGVLNI SDMLGKAMGG RTKTRKTTVK DSYPILINDE SDKLLDQDQI VQEALRVSED EGIVFIDEID KIAAREGGSG AGVSREGVQR DLLPLVEGTT VATKYGPVKT DHILFITSGA FHVSKPSDLL PELQGRLPIR VELSALTRED FRRILTETEA SLIKQYIALM ETEEVKLEFS DDAIDALADI AVDLNATVEN IGARRLQTVI EKVLDEISFT APDKAGATFI IDAAYVKEKI GGLAKNTDLS RFIL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAATTTTTCT CCCCGTGAAA TCGTTTCGGA GCTTGACCGC TTCATCATCG GCCAGAAGGA CGCCAAGCGC GCGGTGGCCA TTGCGCTGCG CAATCGCTGG CGCCGCCAGC AGCTTGAAGG GCAGATGCGC GAAGAGGTCA TGCCGAAGAA TATCCTGATG ATCGGGCCGA CCGGCGTGGG CAAAACGGAA ATCTCGCGCC GTCTGGCCAA GCTTGCAGGC GCGCCCTTCG TCAAGGTGGA GGCCACCAAA TTTACCGAAG TGGGCTATGT CGGCCGCGAT GTGGAGCAGA TCATCCGCGA TCTGGTCGAG ATCGCCATCA CGCTTGTGCG TGAAAAGCGC CGCGAGGACG TGAAGGCCAA GGCGCATCTC AACGCCGAGG AACGGGTTCT GGACGCGCTC GTGGGCAAGA CGGCCAGCCC CGCCACCCGT GACAGCTTCC GCAAGAAGCT GCGCAATGGC GAGATGGACG ACAAGGAAAT CGAGATTGAG GTTTCCGATT CCGGCGCCAG CCCGAACTTC GAGATTCCCG GCATGCCGGG CGCGAATATC GGCGTGCTCA ATATTTCCGA CATGCTGGGC AAGGCCATGG GCGGGCGCAC CAAGACGCGC AAGACCACGG TGAAGGATTC CTACCCCATC CTCATCAATG ACGAATCCGA CAAGCTGCTC GATCAGGACC AGATCGTGCA GGAGGCGCTG CGCGTGAGCG AGGATGAAGG CATCGTCTTC ATCGATGAGA TTGACAAGAT CGCCGCGCGC GAAGGCGGGA GCGGGGCAGG CGTCTCGCGT GAAGGCGTGC AGCGCGACCT GCTACCGCTT GTCGAAGGCA CAACGGTCGC GACCAAATAC GGGCCGGTGA AGACGGATCA CATTCTTTTC ATCACCTCAG GCGCGTTCCA TGTCTCCAAG CCATCCGATC TTCTGCCGGA ATTGCAGGGC CGCCTGCCCA TCCGCGTGGA ACTTTCCGCG CTGACGCGGG AGGATTTCCG CCGCATTCTG ACGGAAACCG AGGCAAGCCT CATCAAGCAG TATATTGCGC TCATGGAAAC GGAAGAGGTG AAGCTTGAAT TCTCCGACGA CGCGATTGAT GCGCTGGCCG ATATCGCGGT CGATCTCAAC GCGACGGTGG AGAATATCGG CGCCCGCCGC CTGCAAACCG TGATAGAGAA GGTGCTGGAC GAAATTTCCT TCACCGCGCC GGACAAGGCC GGAGCAACCT TCATCATCGA CGCAGCCTAT GTGAAGGAGA AGATCGGCGG CCTTGCCAAA AACACCGATC TTTCGCGCTT CATTCTTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA
Details for BrabA.00053.a.A3.PS00521
PURIFICATION DATe: 3/12/2010
CONCENTRATION: 35.42mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 11
VIAL VOLUME: 200µl
PERCENT IDENTITY: 93
PERCENT COVERAGE: 66
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSNFSPREI VSELDRFIIG QKDAKRAVAI ALRNRWRRQQ LEGQMREEVM PKNILMIGPT GVGKTEISRR LAKLAGAPFV KVEATKFTEV GYVGRDVEQI IRDLVEIAIT LVREKRREDV KAKAHLNAEE RVLDALVGKT ASPATRDSFR KKLRNGEMDD KEIEIEVSDS GASPNFEIPG MPGANIGVLN ISDMLGKAMG GRTKTRKTTV KDSYPILIND ESDXLLDQDQ IVXXALRVSE DEGIVFIDEX DKIAAREGGS GAXSRVXAXS ATCYRLS
Validated NT Sequence
ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcaatttt tctccccgtg aaatcgtttc ggagcttgac cgcttcatca tcggccagaa ggacgccaag cgcgcggtgg ccattgcgct gcgcaatcgc tggcgccgcc agcagcttga agggcagatg cgcgaagagg tcatgccgaa gaatatcctg atgatcgggc cgaccggcgt gggcaaaacg gaaatctcgc gccgtctggc caagcttgca ggcgcgccct tcgtcaaggt ggaggccacc aaatttaccg aagtgggcta tgtcggccgc gatgtggagc agatcatccg cgatctggtc gagatcgcca tcacgcttgt gcgtgaaaag cgccgcgagg acgtgaaggc caaggcgcat ctcaacgccg aggaacgggt tctggacgcg ctcgtgggca agacggccag ccccgccacc cgtgacagct tccgcaagaa gctgcgcaat ggcgagatgg acgacaagga aatcgagatt gaggtttccg attccggcgc cagcccgaac ttcgagattc ccggcatgcc gggcgcgaat atcggcgtgc tcaatatttc cgacatgctg ggcaaggcca tgggcgggcg caccaagacg cgcaagacca cggtgaagga ttcctacccc atcctcatca atgacgaatc cgacnagctg ctcgatcagg accagatcgt gcangangcg ctgcgcgtga gcgaggatga aggcatcgtc ttcatcgatg agantgacaa gatcgccgcg cgcgaaggcg ggagcggggc nnngtctcgc gtgaangcgt gnagcgcgac ctgctaccgc ttgtcna
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSNFSPREI VSELDRFIIG QKDAKRAVAI ALRNRWRRQQ LEGQMREEVM PKNILMIGPT GVGKTEISRR LAKLAGAPFV KVEATKFTEV GYVGRDVEQI IRDLVEIAIT LVREKRREDV KAKAHLNAEE RVLDALVGKT ASPATRDSFR KKLRNGEMDD KEIEIEVSDS GASPNFEIPG MPGANIGVLN ISDMLGKAMG GRTKTRKTTV KDSYPILIND ESDKLLDQDQ IVQEALRVSE DEGIVFIDEI DKIAAREGGS GAGVSREGVQ RDLLPLVEGT TVATKYGPVK TDHILFITSG AFHVSKPSDL LPELQGRLPI RVELSALTRE DFRRILTETE ASLIKQYIAL METEEVKLEF SDDAIDALAD IAVDLNATVE NIGARRLQTV IEKVLDEISF TAPDKAGATF IIDAAYVKEK IGGLAKNT
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCAATTTT TCTCCCCGTG AAATCGTTTC GGAGCTTGAC CGCTTCATCA TCGGCCAGAA GGACGCCAAG CGCGCGGTGG CCATTGCGCT GCGCAATCGC TGGCGCCGCC AGCAGCTTGA AGGGCAGATG CGCGAAGAGG TCATGCCGAA GAATATCCTG ATGATCGGGC CGACCGGCGT GGGCAAAACG GAAATCTCGC GCCGTCTGGC CAAGCTTGCA GGCGCGCCCT TCGTCAAGGT GGAGGCCACC AAATTTACCG AAGTGGGCTA TGTCGGCCGC GATGTGGAGC AGATCATCCG CGATCTGGTC GAGATCGCCA TCACGCTTGT GCGTGAAAAG CGCCGCGAGG ACGTGAAGGC CAAGGCGCAT CTCAACGCCG AGGAACGGGT TCTGGACGCG CTCGTGGGCA AGACGGCCAG CCCCGCCACC CGTGACAGCT TCCGCAAGAA GCTGCGCAAT GGCGAGATGG ACGACAAGGA AATCGAGATT GAGGTTTCCG ATTCCGGCGC CAGCCCGAAC TTCGAGATTC CCGGCATGCC GGGCGCGAAT ATCGGCGTGC TCAATATTTC CGACATGCTG GGCAAGGCCA TGGGCGGGCG CACCAAGACG CGCAAGACCA CGGTGAAGGA TTCCTACCCC ATCCTCATCA ATGACGAATC CGACAAGCTG CTCGATCAGG ACCAGATCGT GCAGGAGGCG CTGCGCGTGA GCGAGGATGA AGGCATCGTC TTCATCGATG AGATTGACAA GATCGCCGCG CGCGAAGGCG GGAGCGGGGC AGGCGTCTCG CGTGAAGGCG TGCAGCGCGA CCTGCTACCG CTTGTCGAAG GCACAACGGT CGCGACCAAA TACGGGCCGG TGAAGACGGA TCACATTCTT TTCATCACCT CAGGCGCGTT CCATGTCTCC AAGCCATCCG ATCTTCTGCC GGAATTGCAG GGCCGCCTGC CCATCCGCGT GGAACTTTCC GCGCTGACGC GGGAGGATTT CCGCCGCATT CTGACGGAAA CCGAGGCAAG CCTCATCAAG CAGTATATTG CGCTCATGGA AACGGAAGAG GTGAAGCTTG AATTCTCCGA CGACGCGATT GATGCGCTGG CCGATATCGC GGTCGATCTC AACGCGACGG TGGAGAATAT CGGCGCCCGC CGCCTGCAAA CCGTGATAGA GAAGGTGCTG GACGAAATTT CCTTCACCGC GCCGGACAAG GCCGGAGCAA CCTTCATCAT CGACGCAGCC TATGTGAAGG AGAAGATCGG CGGCCTTGCC AAAAACACCA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A
Details for BrabA.00053.a.A6.PS00531
PURIFICATION DATe: 3/12/2010
CONCENTRATION: 29.6mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 19
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MNFSPREIVS ELDRFIIGQK DAKRAVAIAL RNRWRRQQLE GQMREEVMPK NILMIGPTGV GKTEISRRLA KLAGAPFVKV EATKFTEVGY VGRDVEQIIR DLVEIAITLV REKRREDVKA KAHLNAEERV LDALVGKTAS PATRDSFRKK LRNGEMDDKE IEIEVSDSGA SPNFEIPGMP GANIGVLNIS DMLGKAMGGR TKTRKTTVKD SYPILINDES DKLLDQDQIV QEALRVSEDE GIVFIDEIDK IAAREGGSGA GVSREGVQRD LLPLVEGTTV ATKYGPVKTD HILFITSGAF HVSKPSDLLP ELQGRLPIRV ELSALTREDF RRILTETEAS LIKQYIALME TEEVKLEFSD DAIDALADIA VDLNATVENI GARRLQTVIE KVLDEISFTA PDKAGATFII DAAYVKEKIG GLAK
Validated NT Sequence
ttttggcaag gccgccgatc ttctccttca cataggctgc gtcgatgatg aaggttgctc cggccttgtc cggcgcggtg aaggaaattt cgtccagcac cttctctatc acggtttgca ggcggcgggc gccgatattc tccaccgtcg cgttgagatc gaccgcgata tcggccagcg catcaatcgc gtcgtcggag aattcaagct tcacctcttc cgtttccatg agcgcaatat actgcttgat gaggcttgcc tcggtttccg tcagaatgcg gcggaaatcc tcccgcgtca gcgcggaaag ttccacgcgg atgggcaggc ggccctgcaa ttccggcaga agatcggatg gcttggagac atggaacgcg cctgaggtga tgaaaagaat gtgatccgtc ttcaccggcc cgtatttggt cgcgaccgtt gtgccttcga caagcggtag caggtcgcgc tgcacgcctt cacgcgagac gcctgccccg ctcccgcctt cgcgcgcggc gatcttgtca atctcatcga tgaagacgat gccttcatcc tcgctcacgc gcagcgcctc ctgcacgatc tggtcctgat cgagcagctt gtcggattcg tcattgatga ggatggggta ggaatccttc accgtggtct tgcgcgtctt ggtgcgcccg cccatggcct tgcccagcat gtcggaaata ttgagcacgc cgatattcgc gcccggcatg ccgggaatct cgaagttcgg gctggcgccg gaatcggaaa cctcaatctc gatttccttg tcgtccatct cgccattgcg cagcttcttg cggaagctgt cacgggtggc ggggctggcc gtcttgccca cgagcgcgtc cagaacccgt tcctcggcgt tgagatgcgc cttggccttc acgtcctcgc ggcgcttttc acgcacaagc gtgatggcga tctcgaccag atcgcggatg atctgctcca catcgcggcc gacatagccc acttcggtaa atttggtggc ctccaccttg acgaagggcg cgcctgcaag cttggccaga cggcgcgaga tttccgtttt gcccacgccg gtcggcccga tcatcaggat attcttcggc atgacctctt cgcgcatctg cccttcaagc tgctggcggc gccagcgatt gcgcagcgca atggccaccg cgcgcttggc gtccttctgg ccgatgatga agcggtcaag ctccgaaacg atttcacggg gagaaaaatt catcgaacca ggaccctggg tctgagcttc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNFSPREIV SELDRFIIGQ KDAKRAVAIA LRNRWRRQQL EGQMREEVMP KNILMIGPTG VGKTEISRRL AKLAGAPFVK VEATKFTEVG YVGRDVEQII RDLVEIAITL VREKRREDVK AKAHLNAEER VLDALVGKTA SPATRDSFRK KLRNGEMDDK EIEIEVSDSG ASPNFEIPGM PGANIGVLNI SDMLGKAMGG RTKTRKTTVK DSYPILINDE SDKLLDQDQI VQEALRVSED EGIVFIDEID KIAAREGGSG AGVSREGVQR DLLPLVEGTT VATKYGPVKT DHILFITSGA FHVSKPSDLL PELQGRLPIR VELSALTRED FRRILTETEA SLIKQYIALM ETEEVKLEFS DDAIDALADI AVDLNATVEN IGARRLQTVI EKVLDEISFT APDKAGATFI IDAAYVKEKI GGLAKNT
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAATTTTTCT CCCCGTGAAA TCGTTTCGGA GCTTGACCGC TTCATCATCG GCCAGAAGGA CGCCAAGCGC GCGGTGGCCA TTGCGCTGCG CAATCGCTGG CGCCGCCAGC AGCTTGAAGG GCAGATGCGC GAAGAGGTCA TGCCGAAGAA TATCCTGATG ATCGGGCCGA CCGGCGTGGG CAAAACGGAA ATCTCGCGCC GTCTGGCCAA GCTTGCAGGC GCGCCCTTCG TCAAGGTGGA GGCCACCAAA TTTACCGAAG TGGGCTATGT CGGCCGCGAT GTGGAGCAGA TCATCCGCGA TCTGGTCGAG ATCGCCATCA CGCTTGTGCG TGAAAAGCGC CGCGAGGACG TGAAGGCCAA GGCGCATCTC AACGCCGAGG AACGGGTTCT GGACGCGCTC GTGGGCAAGA CGGCCAGCCC CGCCACCCGT GACAGCTTCC GCAAGAAGCT GCGCAATGGC GAGATGGACG ACAAGGAAAT CGAGATTGAG GTTTCCGATT CCGGCGCCAG CCCGAACTTC GAGATTCCCG GCATGCCGGG CGCGAATATC GGCGTGCTCA ATATTTCCGA CATGCTGGGC AAGGCCATGG GCGGGCGCAC CAAGACGCGC AAGACCACGG TGAAGGATTC CTACCCCATC CTCATCAATG ACGAATCCGA CAAGCTGCTC GATCAGGACC AGATCGTGCA GGAGGCGCTG CGCGTGAGCG AGGATGAAGG CATCGTCTTC ATCGATGAGA TTGACAAGAT CGCCGCGCGC GAAGGCGGGA GCGGGGCAGG CGTCTCGCGT GAAGGCGTGC AGCGCGACCT GCTACCGCTT GTCGAAGGCA CAACGGTCGC GACCAAATAC GGGCCGGTGA AGACGGATCA CATTCTTTTC ATCACCTCAG GCGCGTTCCA TGTCTCCAAG CCATCCGATC TTCTGCCGGA ATTGCAGGGC CGCCTGCCCA TCCGCGTGGA ACTTTCCGCG CTGACGCGGG AGGATTTCCG CCGCATTCTG ACGGAAACCG AGGCAAGCCT CATCAAGCAG TATATTGCGC TCATGGAAAC GGAAGAGGTG AAGCTTGAAT TCTCCGACGA CGCGATTGAT GCGCTGGCCG ATATCGCGGT CGATCTCAAC GCGACGGTGG AGAATATCGG CGCCCGCCGC CTGCAAACCG TGATAGAGAA GGTGCTGGAC GAAATTTCCT TCACCGCGCC GGACAAGGCC GGAGCAACCT TCATCATCGA CGCAGCCTAT GTGAAGGAGA AGATCGGCGG CCTTGCCAAA AACACCAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA
Details for BrabA.00053.a.A2.PS00548
PURIFICATION DATe: 4/7/2010
CONCENTRATION: 34.8mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSNFSPREI VSELDRFIIG QKDAKRAVAI ALRNRWRRQQ LEGQMREEVM PKNILMIGPT GVGKTEISRR LAKLAGAPFV KVEATKFTEV GYVGRDVEQI IRDLVEIAIT LVREKRREDV KAKAHLNAEE RVLDALVGKT ASPATRDSFR KKLRNGEMDD KEIEIEVSDS GASPNFEIPG MPGANIGVLN ISDMLGKAMG GRTKTRKTTV KDSYPILIND ESDKLLDQDQ IVQEALRVSE DEGIVFIDEI DKIAAREGGS GAGVSREGVQ RDLLPLVEGT TVATKYGPVK TDHILFITSG AFHVSKPSDL LPELQGRLPI RVELSALTRE DFRRILTETE ASLIKQYIAL METEEVKLEF SDDAIDALAD IAVDLNATVE NIGARRLQTV IEKVLDEISF TAPDKAGATF IIDAAYVKE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCAATTTT TCTCCCCGTG AAATCGTTTC GGAGCTTGAC CGCTTCATCA TCGGCCAGAA GGACGCCAAG CGCGCGGTGG CCATTGCGCT GCGCAATCGC TGGCGCCGCC AGCAGCTTGA AGGGCAGATG CGCGAAGAGG TCATGCCGAA GAATATCCTG ATGATCGGGC CGACCGGCGT GGGCAAAACG GAAATCTCGC GCCGTCTGGC CAAGCTTGCA GGCGCGCCCT TCGTCAAGGT GGAGGCCACC AAATTTACCG AAGTGGGCTA TGTCGGCCGC GATGTGGAGC AGATCATCCG CGATCTGGTC GAGATCGCCA TCACGCTTGT GCGTGAAAAG CGCCGCGAGG ACGTGAAGGC CAAGGCGCAT CTCAACGCCG AGGAACGGGT TCTGGACGCG CTCGTGGGCA AGACGGCCAG CCCCGCCACC CGTGACAGCT TCCGCAAGAA GCTGCGCAAT GGCGAGATGG ACGACAAGGA AATCGAGATT GAGGTTTCCG ATTCCGGCGC CAGCCCGAAC TTCGAGATTC CCGGCATGCC GGGCGCGAAT ATCGGCGTGC TCAATATTTC CGACATGCTG GGCAAGGCCA TGGGCGGGCG CACCAAGACG CGCAAGACCA CGGTGAAGGA TTCCTACCCC ATCCTCATCA ATGACGAATC CGACAAGCTG CTCGATCAGG ACCAGATCGT GCAGGAGGCG CTGCGCGTGA GCGAGGATGA AGGCATCGTC TTCATCGATG AGATTGACAA GATCGCCGCG CGCGAAGGCG GGAGCGGGGC AGGCGTCTCG CGTGAAGGCG TGCAGCGCGA CCTGCTACCG CTTGTCGAAG GCACAACGGT CGCGACCAAA TACGGGCCGG TGAAGACGGA TCACATTCTT TTCATCACCT CAGGCGCGTT CCATGTCTCC AAGCCATCCG ATCTTCTGCC GGAATTGCAG GGCCGCCTGC CCATCCGCGT GGAACTTTCC GCGCTGACGC GGGAGGATTT CCGCCGCATT CTGACGGAAA CCGAGGCAAG CCTCATCAAG CAGTATATTG CGCTCATGGA AACGGAAGAG GTGAAGCTTG AATTCTCCGA CGACGCGATT GATGCGCTGG CCGATATCGC GGTCGATCTC AACGCGACGG TGGAGAATAT CGGCGCCCGC CGCCTGCAAA CCGTGATAGA GAAGGTGCTG GACGAAATTT CCTTCACCGC GCCGGACAAG GCCGGAGCAA CCTTCATCAT CGACGCAGCC TATGTGAAGG AGAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA