BrabA.00052.a
Glyceraldehyde 3-phosphate dehydrogenase:TrkA potassium uptake protein:Glyceraldehyde-3-phosphate dehydrogenase, type I
| CENTER ID: | BrabA.00052.a |
| ORGANISM: | Brucella melitensis biovar Abortus 2308 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.00052.a.A1.GE25222 | Full length( BrabA.00052.a ) | 1 | 335 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.00052.a.D11.PD00018 | Full length( BrabA.00052.a ) | 1 | 335 |
Structures
3DOC
DEPOSITED: 7/3/2008
DETERMINATION: XRay
CLONE:
PROTEIN: BrabA.00052.a.D11.PD00018
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126628 |
| PATRIC ID: | fig|359391.4.peg.1802 |
| RefSeq: | YP_415091 |
| UniProt: | Q2YRE0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAVRVAINGF GRIGRNILRA IVESGRTDIQ VVAINDLGPV ETNAHLLRYD SVHGRFPKEV EVAGDTIDVG YGPIKVHAVR NPAELPWKEE NVDIALECTG IFTSRDKAAL HLEAGAKRVI VSAPADGADL TVVYGVNNDK LTKDHLVISN ASCTTNCLAP VAQVLNDTIG IEKGFMTTIH SYTGDQPTLD TMHKDLYRAR AAALSMIPTS TGAAKAVGLV LPELKGKLDG VAIRVPTPNV SVVDLTFIAK RETTVEEVNN AIREAANGRL KGILGYTDEK LVSHDFNHDS HSSVFHTDQT KVMDGTMVRI LSWYDNEWGF SSRMSDTAVA LGKLI
NT Sequence
ATGGCAGTTC GCGTCGCAAT CAACGGTTTT GGCCGCATTG GCCGTAACAT CCTTCGCGCC ATCGTGGAAT CGGGGCGCAC CGACATTCAG GTCGTCGCCA TCAACGATCT CGGCCCGGTC GAAACCAACG CACATCTTCT GCGTTATGAC AGCGTTCATG GCCGTTTCCC CAAGGAAGTG GAGGTTGCAG GCGATACGAT CGATGTTGGC TACGGCCCGA TCAAGGTTCA TGCCGTCCGC AACCCGGCCG AACTGCCGTG GAAGGAAGAA AACGTCGATA TCGCCCTTGA ATGCACCGGC ATTTTCACCT CGCGCGACAA GGCAGCACTT CATCTTGAAG CTGGCGCCAA GCGCGTCATC GTCTCCGCTC CCGCAGACGG TGCCGATCTC ACCGTCGTCT ATGGTGTCAA CAACGACAAG CTGACGAAGG ACCATCTGGT CATCTCCAAC GCTTCGTGTA CCACCAACTG CCTTGCGCCG GTGGCTCAGG TTCTCAACGA TACTATCGGT ATCGAAAAGG GCTTCATGAC CACGATCCAC TCCTATACGG GCGACCAGCC GACGCTGGAC ACCATGCACA AGGATCTCTA CCGCGCCCGC GCCGCTGCCC TTTCCATGAT CCCGACCTCG ACGGGTGCGG CCAAGGCCGT CGGTCTCGTT CTGCCGGAAC TGAAAGGCAA GCTCGACGGC GTTGCCATTC GCGTCCCGAC CCCAAATGTC TCGGTCGTTG ATCTCACCTT CATCGCCAAG CGTGAAACCA CCGTTGAAGA AGTCAACAAT GCGATCCGCG AAGCCGCCAA TGGCCGCCTC AAGGGCATTC TCGGCTATAC CGATGAGAAG CTCGTCTCGC ACGACTTCAA CCACGATTCC CATTCCTCGG TCTTCCACAC CGACCAGACC AAGGTTATGG ACGGCACCAT GGTGCGTATC CTGTCGTGGT ACGACAATGA ATGGGGCTTC TCCAGCCGCA TGAGCGACAC CGCCGTCGCT TTGGGCAAGC TGATC
Details for BrabA.00052.a.A1.GE25222
| HARVESTED ON: | 5/6/2008 |
| SEQUENCED ON: | 6/16/2009 |
| EXPECTED MW: | 38kDa |
| OBSERVED MW: | 39kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 89 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAVRVAING FGRIGRNILR AIVESGRTDI QVVAINDLGP VETNAHLLRY DSVHGRFPKE VEVAGDTIDV GYGPIKVHAV RNPAELPWKE ENVDIALECT GIFTSRDKAA LHLEAGAKRV IVSAPADGAD LTVVYGVNND KLTKDHLVIS NASCTTNCLA PVAQVLNDTI GIEKGFMTTI HSYTGDQPTL DTMHKDLYRA RAAALSMIPT STGAAKAVGL VLPELKGKLD GVAIRVPTPN VSVVDLTFIA KRETTVEEVN NAIREAANGR LXGILGYTDX KLVSHDFNHD XXSSVFHTD
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcag ttcgcgtcgc aatcaacggt tttggccgca ttggccgtaa catccttcgc gccatcgtgg aatcggggcg caccgacatt caggtcgtcg ccatcaacga tctcggcccg gtcgaaacca acgcacatct tctgcgttat gacagcgttc atggccgttt ccccaaggaa gtggaggttg caggcgatac gatcgatgtt ggctacggcc cgatcaaggt tcatgccgtc cgcaacccgg ccgaactgcc gtggaaggaa gaaaacgtcg atatcgccct tgaatgcacc ggcattttca cctcgcgcga caaggcagca cttcatcttg aagctggcgc caagcgcgtc atcgtctccg ctcccgcaga cggtgccgat ctcaccgtcg tctatggtgt caacaacgac aagctgacga aggaccatct ggtcatctcc aacgcttcgt gtaccaccaa ctgccttgcg ccggtggctc aggttctcaa cgatactatc ggtatcgaaa agggcttcat gaccacgatc cactcctata cgggcgacca gccgacgctg gacaccatgc acaaggatct ctaccgcgcc cgcgccgctg ccctttccat gatcccgacc tcgacgggtg cggccaaggc cgtcggtctc gttctgccgg aactgaaagg caagctcgac ggcgttgcca ttcgcgtccc gaccccnaat gtctcggtcg ttgatctcac cttcatcgcc aagcgtgaaa ccaccgttga agaagtcaac aatgcgatcc gcgaagccgc caatggncgc ctcangggna ttctcggcta taccgatgan aagctcgtct cgcacgactt caaccacgat tnccnttcct cggtcttcca caccgacca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAVRVAING FGRIGRNILR AIVESGRTDI QVVAINDLGP VETNAHLLRY DSVHGRFPKE VEVAGDTIDV GYGPIKVHAV RNPAELPWKE ENVDIALECT GIFTSRDKAA LHLEAGAKRV IVSAPADGAD LTVVYGVNND KLTKDHLVIS NASCTTNCLA PVAQVLNDTI GIEKGFMTTI HSYTGDQPTL DTMHKDLYRA RAAALSMIPT STGAAKAVGL VLPELKGKLD GVAIRVPTPN VSVVDLTFIA KRETTVEEVN NAIREAANGR LKGILGYTDE KLVSHDFNHD SHSSVFHTDQ TKVMDGTMVR ILSWYDNEWG FSSRMSDTAV ALGKLI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcagttcgc gtcgcaatca acggttttgg ccgcattggc cgtaacatcc ttcgcgccat cgtggaatcg gggcgcaccg acattcaggt cgtcgccatc aacgatctcg gcccggtcga aaccaacgca catcttctgc gttatgacag cgttcatggc cgtttcccca aggaagtgga ggttgcaggc gatacgatcg atgttggcta cggcccgatc aaggttcatg ccgtccgcaa cccggccgaa ctgccgtgga aggaagaaaa cgtcgatatc gcccttgaat gcaccggcat tttcacctcg cgcgacaagg cagcacttca tcttgaagct ggcgccaagc gcgtcatcgt ctccgctccc gcagacggtg ccgatctcac cgtcgtctat ggtgtcaaca acgacaagct gacgaaggac catctggtca tctccaacgc ttcgtgtacc accaactgcc ttgcgccggt ggctcaggtt ctcaacgata ctatcggtat cgaaaagggc ttcatgacca cgatccactc ctatacgggc gaccagccga cgctggacac catgcacaag gatctctacc gcgcccgcgc cgctgccctt tccatgatcc cgacctcgac gggtgcggcc aaggccgtcg gtctcgttct gccggaactg aaaggcaagc tcgacggcgt tgccattcgc gtcccgaccc caaatgtctc ggtcgttgat ctcaccttca tcgccaagcg tgaaaccacc gttgaagaag tcaacaatgc gatccgcgaa gccgccaatg gccgcctcaa gggcattctc ggctataccg atgagaagct cgtctcgcac gacttcaacc acgattccca ttcctcggtc ttccacaccg accagaccaa ggttatggac ggcaccatgg tgcgtatcct gtcgtggtac gacaatgaat ggggcttctc cagccgcatg agcgacaccg ccgtcgcttt gggcaagctg atctgaaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BrabA.00052.a.D11.PD00018
| PURIFICATION DATe: | 6/11/2008 |
| CONCENTRATION: | 19.6mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 60 |
| VIAL VOLUME: | 50µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSMAV RVAINGFGRI GRNILRAIVE SGRTDIQVVA INDLGPVETN AHLLRYDSVH GRFPKEVEVA GDTIDVGYGP IKVHAVRNPA ELPWKEENVD IALECTGIFT SRDKAALHLE AGAKRVIVSA PADGADLTVV YGVNNDKLTK DHLVISNASC TTNCLAPVAQ VLNDTIGIEK GFMTTIHSYT GDQPTLDTMH KDLYRARAAA LSMIPTSTGA AKAVGLVLPE LKGKLDGVAI RVPTPNVSVV DLTFIAKRET TVEEVNNAIR EAANGRLKGI LGYTDEKLVS HDFNHDSHSS VFHTDQTKVM DGTMVRILSW YDNEWGFSSR MSDTAVALGK LI
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCCACC ACCACCACCA CCATAGCGGC GAAGTAAAAC CGGAAGTGAA GCCGGAGACC CACATCAACC TGAAGGTTAG CGACGGTAGC AGCGAAATCT TCTTCAAGAT TAAGAAGACC ACCCCGCTGC GTCGCCTGAT GGAAGCATTC GCTAAACGCC AGGGCAAGGA AATGGATTCC CTGCGCTTTC TGTACGACGG TATCCGTATT CAGGCAGACC AGACTCCGGA AGATCTGGAC ATGGAAGATA ACGACATTAT CGAAGCACAC CGTGAACAAA TCGGTGGATC CATGGCAGTT CGCGTGGCCA TCAACGGTTT CGGCCGCATT GGCCGTAACA TCCTTCGTGC AATTGTGGAA TCCGGCCGTA CTGATATTCA GGTAGTTGCT ATCAACGACT TGGGCCCTGT TGAAACCAAC GCCCATTTAC TCCGTTACGA CTCCGTACAC GGCCGTTTCC CGAAAGAGGT TGAAGTAGCT GGCGACACCA TCGACGTGGG TTACGGTCCG ATCAAAGTCC ACGCAGTGCG TAACCCGGCC GAACTGCCGT GGAAGGAGGA GAATGTTGAC ATAGCTCTGG AGTGTACCGG CATCTTTACT AGCCGTGATA AAGCTGCACT CCACCTCGAG GCTGGCGCTA AACGTGTTAT AGTTTCCGCA CCAGCAGATG GCGCTGACCT TACCGTGGTT TACGGCGTTA ACAACGATAA ACTGACCAAA GACCACTTAG TGATCTCTAA CGCTTCTTGT ACTACGAACT GCCTCGCACC GGTGGCACAG GTTCTGAACG ATACTATCGG TATTGAGAAA GGCTTCATGA CTACTATCCA CAGCTACACC GGTGATCAGC CGACTCTCGA TACGATGCAC AAAGATCTCT ACCGCGCTCG CGCTGCAGCT CTCTCGATGA TCCCAACTAG CACTGGTGCA GCCAAGGCCG TTGGTCTGGT ACTCCCTGAG CTGAAGGGCA AACTCGACGG CGTTGCTATC CGCGTGCCTA CTCCGAATGT GTCCGTTGTT GACCTGACCT TTATTGCAAA GCGTGAAACC ACCGTTGAGG AAGTTAACAA CGCGATCCGT GAAGCAGCCA ACGGTCGTCT GAAAGGTATC CTTGGCTACA CCGACGAGAA ACTGGTATCT CACGACTTCA ACCACGATTC TCACTCTTCC GTGTTCCACA CTGATCAAAC TAAAGTTATG GATGGTACTA TGGTTCGCAT CCTCTCCTGG TACGATAATG AATGGGGTTT CTCTTCCCGC ATGTCTGATA CTGCCGTAGC CCTGGGTAAG CTGATCTAAA AGCTTGCGGC CGCACTCGAG CACCACCACC ACCACCACTG AGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGAT