BrabA.00047.a
Fumarate lyase:Delta crystallin
| CENTER ID: | BrabA.00047.a |
| ORGANISM: | Brucella melitensis biovar Abortus 2308 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.00047.a.A1.GE25221 | Full length( BrabA.00047.a ) | 1 | 483 | ||
| BrabA.00047.a.A2.GE28030 | Created from Decode construct for feature BrabA.00047.a | 1 | 466 | ||
| BrabA.00047.a.A3.GE28031 | Created from Decode construct for feature BrabA.00047.a | 1 | 479 | ||
| BrabA.00047.a.A4.GE28032 | Created from Decode construct for feature BrabA.00047.a | 11 | 483 | ||
| BrabA.00047.a.A5.GE28033 | Created from Decode construct for feature BrabA.00047.a | 11 | 466 | ||
| BrabA.00047.a.A6.GE28034 | Created from Decode construct for feature BrabA.00047.a | 11 | 479 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.00047.a.D19.PD00037 | Full length( BrabA.00047.a ) | 1 | 483 | ||
| BrabA.00047.a.A4.PS00510 | Created from Decode construct for feature BrabA.00047.a | 11 | 483 | ||
| BrabA.00047.a.A5.PS00511 | Created from Decode construct for feature BrabA.00047.a | 11 | 466 | ||
| BrabA.00047.a.A6.PS00513 | Created from Decode construct for feature BrabA.00047.a | 11 | 479 | ||
| BrabA.00047.a.A3.PS00519 | Created from Decode construct for feature BrabA.00047.a | 1 | 479 | ||
| BrabA.00047.a.A2.PS00524 | Created from Decode construct for feature BrabA.00047.a | 1 | 466 |
Structures
3OCF
DEPOSITED: 8/9/2010
DETERMINATION: XRay
CLONE: BrabA.00047.a.A5.GE28033
PROTEIN: BrabA.00047.a.A5.PS00511
3OCE
DEPOSITED: 8/9/2010
DETERMINATION: XRay
CLONE: BrabA.00047.a.A6.GE28034
PROTEIN: BrabA.00047.a.A6.PS00513
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127258 |
| PATRIC ID: | fig|359391.4.peg.2039 |
| RefSeq: | YP_415293 |
| UniProt: | Q2YLW1 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTLENIHPL TRREQDSLGE RDIPMDAYFG IQTLRAVENF SLSDVALNHI PALVRALAMV KKAAATANYK LRQLPEPKYA AIVAACDDII DGLLMEQFVV DVFQGGAGTS SNMNANEVIA NRALEHLGRP RGDYQTIHPN DDVNMSQSTN DVYPTAVRLA LLLSQNQVQT ALHRLIAAFE AKGREFATVI KIGRTQLQDA VPITLGQEFE AFAATLREDT ARLEEVAALF REVNLGGTAI GTRINASHAY AEQAIVELSQ ISGIELKATG NLVEASWDTG AFVTFSGILR RIAVKLSKIA NDLRLLSSGP RSGLGEIRLP AVQPGSSIMP GKVNPVIPES VNQVCYQVIG NDLTVTMAAE SGQLQLNAFE PLIVYNILSS MRLLGRAMTN LAERCVDGIE ANVERCRAGA EESISLATAL VPVVGYARAA EIAKQALASG QTVMEVAISK GLDASALTIM LDPLRMAFPP ETHDKEAHHA SVG
NT Sequence
ATGACAACCC TGGAAAATAT TCATCCCCTG ACACGGCGCG AGCAGGATTC CCTTGGGGAG CGCGATATTC CCATGGATGC CTATTTCGGC ATCCAGACCC TACGGGCAGT TGAAAACTTC TCGCTTTCCG ATGTTGCGCT GAACCATATT CCTGCCCTCG TTCGCGCCCT TGCAATGGTC AAGAAAGCGG CTGCCACCGC CAATTACAAG CTGCGGCAGC TGCCGGAGCC CAAATATGCG GCCATCGTCG CCGCCTGCGA CGACATCATT GACGGGCTTT TGATGGAGCA ATTCGTCGTG GATGTGTTTC AGGGCGGCGC GGGCACCTCA AGCAATATGA ACGCAAATGA GGTTATCGCA AACCGCGCCC TTGAACATCT GGGGCGGCCG CGAGGCGACT ACCAGACGAT CCATCCCAAT GATGACGTCA ACATGTCGCA GTCCACCAAT GATGTGTATC CAACCGCGGT AAGGCTGGCG CTTCTTCTCA GCCAGAACCA GGTTCAGACG GCGCTGCACC GGCTGATTGC CGCCTTTGAA GCAAAGGGGC GGGAATTCGC GACGGTCATC AAGATCGGGC GCACACAGTT GCAGGATGCG GTTCCGATCA CTCTTGGACA GGAGTTCGAG GCATTTGCCG CAACGTTGCG GGAGGACACA GCCCGGCTGG AGGAGGTTGC CGCGCTTTTC CGCGAAGTAA ACCTCGGTGG AACCGCTATC GGCACGCGCA TCAACGCCTC GCACGCCTAT GCCGAACAGG CCATCGTGGA ACTTTCGCAG ATTTCCGGTA TCGAACTGAA AGCCACCGGC AATCTCGTGG AAGCGAGCTG GGATACCGGT GCGTTCGTCA CCTTTTCCGG GATATTGCGC CGGATCGCAG TCAAGCTTTC CAAGATCGCC AACGATCTGC GGCTACTCTC AAGCGGGCCG CGAAGCGGCC TTGGTGAAAT CCGCCTGCCT GCGGTTCAGC CGGGATCGTC GATCATGCCG GGAAAGGTCA ATCCGGTCAT ACCGGAATCG GTCAATCAGG TCTGCTATCA GGTGATCGGC AATGACCTAA CTGTAACCAT GGCAGCCGAA AGCGGGCAAT TGCAGCTCAA CGCGTTCGAG CCGCTGATCG TCTACAATAT CCTCAGTTCC ATGCGACTGC TCGGCCGCGC CATGACCAAT CTGGCCGAAC GTTGCGTCGA CGGAATTGAA GCCAATGTGG AACGCTGTCG CGCAGGCGCG GAGGAAAGCA TTTCGCTTGC AACCGCCCTC GTCCCGGTCG TGGGCTATGC CAGGGCGGCA GAGATCGCCA AGCAGGCGCT TGCCTCAGGG CAGACCGTCA TGGAAGTGGC GATTTCCAAG GGGCTGGACG CATCTGCGCT CACGATAATG CTTGACCCCT TGAGAATGGC ATTCCCGCCG GAAACGCACG ACAAGGAGGC ACATCATGCA TCTGTCGGC
Details for BrabA.00047.a.A1.GE25221
| HARVESTED ON: | 5/6/2008 |
| SEQUENCED ON: | 6/16/2009 |
| EXPECTED MW: | 54kDa |
| OBSERVED MW: | 55kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 97 |
| PERCENT COVERAGE: | 60 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTTLENIHP LTRREQDSLG ERDIPMDAYF GIQTLRAVEN FSLSDVALNH IPALVRALAM VKKAAATANY KLRQLPEPKY AAIVAACDDI IDGLLMEQFV VDVFQGGAGT SSNMNANEVI ANRALEHLGR PRGDYQTIHP NDDVNMSQST NDVYPTAVRL ALLLSQNQVQ TALHRLIAAF EAKGREFATV IKIGRTQLQD AVPITLGQEF EAFAATLRED TARLEEVAAL FREVNLGGTA IGTRINASHA YAEQAIXELS QXSGIELKAX GNLXXXSWXX GXFVT
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacaa ccctggaaaa tattcatccc ctgacacggc gcgagcagga ttcccttggg gagcgcgata ttcccatgga tgcctatttc ggcatccaga ccctacgggc agttgaaaac ttctcgcttt ccgatgttgc gctgaaccat attcctgccc tcgttcgcgc ccttgcaatg gtcaagaaag cggctgccac cgccaattac aagctgcggc agctgccgga gcccaaatat gcggccatcg tcgccgcctg cgacgacatc attgacgggc ttttgatgga gcaattcgtc gtggatgtgt ttcagggcgg cgcgggcacc tcaagcaata tgaacgcaaa tgaggttatc gcaaaccgcg ccctcgagca tctggggcgg ccgcgaggcg actaccagac gatccatccc aatgatgacg tcaacatgtc gcagtccacc aatgatgtgt atccaaccgc ggtaaggctg gcgcttcttc tcagccagaa ccaggttcag acggcgctgc accggctgat tgccgccttt gaagcaaagg ggcgggaatt cgcgacggtc atcaagatcg ggcgcacaca gttgcaggat gcggttccga tcactcttgg acaggagttc gaggcatttg ccgcaacgtt gcgggaggac acagcccggc tggaggaggt tgccgcgctt ttccgcgaag taaacctcgg tggaaccgct atcggcacgc gcatcaacgc ctcgcacgcc tatgccgaac aggccatcgn ggaactttcg caganttccg gtatcgaact gaaagccncc ggcaatctcg ngnnancgag ctgggananc ggnncgttcg tcaccttt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTTLENIHP LTRREQDSLG ERDIPMDAYF GIQTLRAVEN FSLSDVALNH IPALVRALAM VKKAAATANY KLRQLPEPKY AAIVAACDDI IDGLLMEQFV VDVFQGGAGT SSNMNANEVI ANRALEHLGR PRGDYQTIHP NDDVNMSQST NDVYPTAVRL ALLLSQNQVQ TALHRLIAAF EAKGREFATV IKIGRTQLQD AVPITLGQEF EAFAATLRED TARLEEVAAL FREVNLGGTA IGTRINASHA YAEQAIVELS QISGIELKAT GNLVEASWDT GAFVTFSGIL RRIAVKLSKI ANDLRLLSSG PRSGLGEIRL PAVQPGSSIM PGKVNPVIPE SVNQVCYQVI GNDLTVTMAA ESGQLQLNAF EPLIVYNILS SMRLLGRAMT NLAERCVDGI EANVERCRAG AEESISLATA LVPVVGYARA AEIAKQALAS GQTVMEVAIS KGLDASALTI MLDPLRMAFP PETHDKEAHH ASVG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacaaccctg gaaaatattc atcccctgac acggcgcgag caggattccc ttggggagcg cgatattccc atggatgcct atttcggcat ccagacccta cgggcagttg aaaacttctc gctttccgat gttgcgctga accatattcc tgccctcgtt cgcgcccttg caatggtcaa gaaagcggct gccaccgcca attacaagct gcggcagctg ccggagccca aatatgcggc catcgtcgcc gcctgcgacg acatcattga cgggcttttg atggagcaat tcgtcgtgga tgtgtttcag ggcggcgcgg gcacctcaag caatatgaac gcaaatgagg ttatcgcaaa ccgcgccctc gagcatctgg ggcggccgcg aggcgactac cagacgatcc atcccaatga tgacgtcaac atgtcgcagt ccaccaatga tgtgtatcca accgcggtaa ggctggcgct tcttctcagc cagaaccagg ttcagacggc gctgcaccgg ctgattgccg cctttgaagc aaaggggcgg gaattcgcga cggtcatcaa gatcgggcgc acacagttgc aggatgcggt tccgatcact cttggacagg agttcgaggc atttgccgca acgttgcggg aggacacagc ccggctggag gaggttgccg cgcttttccg cgaagtaaac ctcggtggaa ccgctatcgg cacgcgcatc aacgcctcgc acgcctatgc cgaacaggcc atcgtggaac tttcgcagat ttccggtatc gaactgaaag ccaccggcaa tctcgtggaa gcgagctggg ataccggtgc gttcgtcacc ttttccggga tattgcgccg gatcgcagtc aagctgtcca agatcgccaa cgatctgcgg ctactctcaa gcgggccgcg aagcggcctt ggtgaaatcc gcctgcctgc ggttcagccg ggatcgtcga tcatgccggg aaaggtcaat ccggtcatac cggaatcggt caatcaggtc tgctatcagg tgatcggcaa tgacctaact gtaaccatgg cagccgaaag cgggcaattg cagctcaacg cgttcgagcc gctgatcgtc tacaatatcc tcagttccat gcgactgctc ggccgcgcca tgaccaatct ggccgaacgt tgcgtcgacg gaattgaagc caatgtggaa cgctgtcgcg caggcgcgga ggaaagcatt tcgcttgcaa ccgccctcgt cccggtcgtg ggctatgcca gggcggcaga gatcgccaag caggcgcttg cctcagggca gaccgtcatg gaagtggcga tttccaaggg gctggacgca tctgcgctca cgataatgct tgaccccttg agaatggcat tcccgccgga aacgcacgac aaggaggcac atcatgcatc tgtcggctaa aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for BrabA.00047.a.A2.GE28030
| HARVESTED ON: | 12/7/2009 |
| SEQUENCED ON: | 12/23/2009 |
| EXPECTED MW: | 52kDa |
| OBSERVED MW: | 48kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTTLENIHP LTRREQDSLG ERDIPMDAYF GIQTLRAVEN FSLSDVALNH IPALVRALAM VKKAAATANY KLRQLPEPKY AAIVAACDDI IDGLLMEQFV VDVFQGGAGT SSNMNANEVI ANRALEHLGR PRGDYQTIHP NDDVNMSQST NDVYPTAVRL ALLLSQNQVQ TALHRLIAAF EAKGREFATV IKIGRTQLQD AVPITLGQEF EAFAATLRED TARLEEVAAL FREVNLGGTA IGTRINASHA YAEQAIVELS QISGIELKAT GNLVEASWDT GAFVTFSGIL RRIAVKLSKI ANDLRLLSSG PRSGLGEIRL PAVQPGSSIM PGKVNPVIPE SVNQVCYQVI GNDLTVTMAA ESGQLQLNAF EPLIVYNILS SMRLLGRAMT NLAERCVDGI EANVERCRAG AEESISLATA LVPVVGYARA AEIAKQALAS GQTVMEVAIS KGLDASALTI MLDPLRM
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacaaccctg gaaaatattc atcccctgac acggcgcgag caggattccc ttggggagcg cgatattccc atggatgcct atttcggcat ccagacccta cgggcagttg aaaacttctc gctttccgat gttgcgctga accatattcc tgccctcgtt cgcgcccttg caatggtcaa gaaagcggct gccaccgcca attacaagct gcggcagctg ccggagccca aatatgcggc catcgtcgcc gcctgcgacg acatcattga cgggcttttg atggagcaat tcgtcgtgga tgtgtttcag ggcggcgcgg gcacctcaag caatatgaac gcaaatgagg ttatcgcaaa ccgcgccctt gaacatctgg ggcggccgcg aggcgactac cagacgatcc atcccaatga tgacgtcaac atgtcgcagt ccaccaatga tgtgtatcca accgcggtaa ggctggcgct tcttctcagc cagaaccagg ttcagacggc gctgcaccgg ctgattgccg cctttgaagc aaaggggcgg gaattcgcga cggtcatcaa gatcgggcgc acacagttgc aggatgcggt tccgatcact cttggacagg agttcgaggc atttgccgca acgttgcggg aggacacagc ccggctggag gaggttgccg cgcttttccg cgaagtaaac ctcggtggaa ccgctatcgg cacgcgcatc aacgcctcgc acgcctatgc cgaacaggcc atcgtggaac tttcgcagat ttccggtatc gaactgaaag ccaccggcaa tctcgtggaa gcgagctggg ataccggtgc gttcgtcacc ttttccggga tattgcgccg gatcgcagtc aagctttcca agatcgccaa cgatctgcgg ctactctcaa gcgggccgcg aagcggcctt ggtgaaatcc gcctgcctgc ggttcagccg ggatcgtcga tcatgccggg aaaggtcaat ccggtcatac cggaatcggt caatcaggtc tgctatcagg tgatcggcaa tgacctaact gtaaccatgg cagccgaaag cgggcaattg cagctcaacg cgttcgagcc gctgatcgtc tacaatatcc tcagttccat gcgactgctc ggccgcgcca tgaccaatct ggccgaacgt tgcgtcgacg gaattgaagc caatgtggaa cgctgtcgcg caggcgcgga ggaaagcatt tcgcttgcaa ccgccctcgt cccggtcgtg ggctatgcca gggcggcaga gatcgccaag caggcgcttg cctcagggca gaccgtcatg gaagtggcga tttccaaggg gctggacgca tctgcgctca cgataatgct tgaccccttg agaatgaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BrabA.00047.a.A3.GE28031
| HARVESTED ON: | 12/7/2009 |
| SEQUENCED ON: | 12/23/2009 |
| EXPECTED MW: | 53kDa |
| OBSERVED MW: | 49kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 76 |
| PERCENT COVERAGE: | 80 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTTLENIHP LTRREQDSLG ERDIPMDAYF GIQTLRAVEN FSLSDVALNH IPALVRALAM VKKAAATANY KLRQLPEPKY AAIVAACDDI IDGLLMEQFV VDVFQGGAGT SSNMNANEVI ANRALEHLGR PRGDYQTIHP NDDVNMSQST NDVYPTAVRL ALLLSQNQVQ TALHRLIAAF EAKGREFATV IKIGRTQLQD AVPITLGQEF EAFAATLRXD TARLEEVAAL FREVNLGGTA IGTRINASHA YAEQAIVELS QISGIELKAT GXLVXRAGIP VRSSPFPGYC AGSQSSFXRS PTICGXSQAG XEXXLVKSAX LXXXXXRXSX XXXXSXXXXX XXXXXXXXXS XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX X
Validated NT Sequence
annnnnttnn nttnacttta agaaggagan ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacaaccctg gaaaatattc atcccctgac acggcgcgag caggattccc ttggggagcg cgatattccc atggatgcct atttcggcat ccagacccta cgggcagttg aaaacttctc gctttccgat gttgcgctga accatattcc tgccctcgtt cgcgcccttg caatggtcaa gaaagcggct gccaccgcca attacaagct gcggcagctg ccggagccca aatatgcggc catcgtcgcc gcctgcgacg acatcattga cgggcttttg atggagcaat tcgtcgtgga tgtgtttcag ggcggcgcgg gcacctcaag caatatgaac gcaaatgagg ttatcgcaaa ccgcgccctt gaacatctgg ggcggccgcg aggcgactac cagacgatcc atcccaatga tgacgtcaac atgtcgcagt ccaccaatga tgtgtatcca accgcggtaa ggctggcgct tcttctcagc cagaaccagg ttcagacggc gctgcaccgg ctgattgccg cctttgaagc aaaggggcgg gaattcgcga cggtcatcaa gatcgggcgc acacagttgc aggatgcggt tccgatcact cttggacagg agttcgaggc atttgccgca acgttgcggg angacacagc ccggctggag gaggttgccg cgcttttccg cgaagtaaac ctcggtggaa ccgctatcgg cacgcgcatc aacgcctcgc acgcctatgc cgaacaggcc atcgtggaac tttcgcagat ttccggtatc gaactgaaag ccaccggcan tctcgtnnng cgagctggga taccggtgcg ttcgtcacct tttccgggat attgcgccgg atcgcagtca agctttncaa gatcgccaac gatctgcggc tantctcaag cgggcnncga ancnnnnttg gtgaaatccg ccnnnctnnn gntcanccng gnncgtcnat catgnnnnna angncnatcc ngtnnnancn gnannngtca ncnngnnnnn nnnnnnngnn gatcngnnan nnanntnnnn nnnnnntgnn nnnnnnnnnn ngnnnannnn nnnnnnnnnn nnnnnntnnn nnnnnncnnt tnnnnnnnnn nnnnnnnnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTTLENIHP LTRREQDSLG ERDIPMDAYF GIQTLRAVEN FSLSDVALNH IPALVRALAM VKKAAATANY KLRQLPEPKY AAIVAACDDI IDGLLMEQFV VDVFQGGAGT SSNMNANEVI ANRALEHLGR PRGDYQTIHP NDDVNMSQST NDVYPTAVRL ALLLSQNQVQ TALHRLIAAF EAKGREFATV IKIGRTQLQD AVPITLGQEF EAFAATLRED TARLEEVAAL FREVNLGGTA IGTRINASHA YAEQAIVELS QISGIELKAT GNLVEASWDT GAFVTFSGIL RRIAVKLSKI ANDLRLLSSG PRSGLGEIRL PAVQPGSSIM PGKVNPVIPE SVNQVCYQVI GNDLTVTMAA ESGQLQLNAF EPLIVYNILS SMRLLGRAMT NLAERCVDGI EANVERCRAG AEESISLATA LVPVVGYARA AEIAKQALAS GQTVMEVAIS KGLDASALTI MLDPLRMAFP PETHDKEAHH
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacaaccctg gaaaatattc atcccctgac acggcgcgag caggattccc ttggggagcg cgatattccc atggatgcct atttcggcat ccagacccta cgggcagttg aaaacttctc gctttccgat gttgcgctga accatattcc tgccctcgtt cgcgcccttg caatggtcaa gaaagcggct gccaccgcca attacaagct gcggcagctg ccggagccca aatatgcggc catcgtcgcc gcctgcgacg acatcattga cgggcttttg atggagcaat tcgtcgtgga tgtgtttcag ggcggcgcgg gcacctcaag caatatgaac gcaaatgagg ttatcgcaaa ccgcgccctt gaacatctgg ggcggccgcg aggcgactac cagacgatcc atcccaatga tgacgtcaac atgtcgcagt ccaccaatga tgtgtatcca accgcggtaa ggctggcgct tcttctcagc cagaaccagg ttcagacggc gctgcaccgg ctgattgccg cctttgaagc aaaggggcgg gaattcgcga cggtcatcaa gatcgggcgc acacagttgc aggatgcggt tccgatcact cttggacagg agttcgaggc atttgccgca acgttgcggg aggacacagc ccggctggag gaggttgccg cgcttttccg cgaagtaaac ctcggtggaa ccgctatcgg cacgcgcatc aacgcctcgc acgcctatgc cgaacaggcc atcgtggaac tttcgcagat ttccggtatc gaactgaaag ccaccggcaa tctcgtggaa gcgagctggg ataccggtgc gttcgtcacc ttttccggga tattgcgccg gatcgcagtc aagctttcca agatcgccaa cgatctgcgg ctactctcaa gcgggccgcg aagcggcctt ggtgaaatcc gcctgcctgc ggttcagccg ggatcgtcga tcatgccggg aaaggtcaat ccggtcatac cggaatcggt caatcaggtc tgctatcagg tgatcggcaa tgacctaact gtaaccatgg cagccgaaag cgggcaattg cagctcaacg cgttcgagcc gctgatcgtc tacaatatcc tcagttccat gcgactgctc ggccgcgcca tgaccaatct ggccgaacgt tgcgtcgacg gaattgaagc caatgtggaa cgctgtcgcg caggcgcgga ggaaagcatt tcgcttgcaa ccgccctcgt cccggtcgtg ggctatgcca gggcggcaga gatcgccaag caggcgcttg cctcagggca gaccgtcatg gaagtggcga tttccaaggg gctggacgca tctgcgctca cgataatgct tgaccccttg agaatggcat tcccgccgga aacgcacgac aaggaggcac atcataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BrabA.00047.a.A4.GE28032
| HARVESTED ON: | 12/7/2009 |
| SEQUENCED ON: | 12/23/2009 |
| EXPECTED MW: | 53kDa |
| OBSERVED MW: | 49kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTRREQDSL GERDIPMDAY FGIQTLRAVE NFSLSDVALN HIPALVRALA MVKKAAATAN YKLRQLPEPK YAAIVAACDD IIDGLLMEQF VVDVFQGGAG TSSNMNANEV IANRALEHLG RPRGDYQTIH PNDDVNMSQS TNDVYPTAVR LALLLSQNQV QTALHRLIAA FEAKGREFAT VIKIGRTQLQ DAVPITLGQE FEAFAATLRE DTARLEEVAA LFREVNLGGT AIGTRINASH AYAEQAIVEL SQISGIELKA TGNLVEASWD TGAFVTFSGI LRRIAVKLSK IANDLRLLSS GPRSGLGEIR LPAVQPGSSI MPGKVNPVIP ESVNQVCYQV IGNDLTVTMA AESGQLQLNA FEPLIVYNIL SSMRLLGRAM TNLAERCVDG IEANVERCRA GAEESISLAT ALVPVVGYAR AAEIAKQALA SGQTVMEVAI SKGLDASALT IMLDPLRMAF PPETHDKEAH HASVG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacacggcgc gagcaggatt cccttgggga gcgcgatatt cccatggatg cctatttcgg catccagacc ctacgggcag ttgaaaactt ctcgctttcc gatgttgcgc tgaaccatat tcctgccctc gttcgcgccc ttgcaatggt caagaaagcg gctgccaccg ccaattacaa gctgcggcag ctgccggagc ccaaatatgc ggccatcgtc gccgcctgcg acgacatcat tgacgggctt ttgatggagc aattcgtcgt ggatgtgttt cagggcggcg cgggcacctc aagcaatatg aacgcaaatg aggttatcgc aaaccgcgcc cttgaacatc tggggcggcc gcgaggcgac taccagacga tccatcccaa tgatgacgtc aacatgtcgc agtccaccaa tgatgtgtat ccaaccgcgg taaggctggc gcttcttctc agccagaacc aggttcagac ggcgctgcac cggctgattg ccgcctttga agcaaagggg cgggaattcg cgacggtcat caagatcggg cgcacacagt tgcaggatgc ggttccgatc actcttggac aggagttcga ggcatttgcc gcaacgttgc gggaggacac agcccggctg gaggaggttg ccgcgctttt ccgcgaagta aacctcggtg gaaccgctat cggcacgcgc atcaacgcct cgcacgccta tgccgaacag gccatcgtgg aactttcgca gatttccggt atcgaactga aagccaccgg caatctcgtg gaagcgagct gggataccgg tgcgttcgtc accttttccg ggatattgcg ccggatcgca gtcaagcttt ccaagatcgc caacgatctg cggctactct caagcgggcc gcgaagcggc cttggtgaaa tccgcctgcc tgcggttcag ccgggatcgt cgatcatgcc gggaaaggtc aatccggtca taccggaatc ggtcaatcag gtctgctatc aggtgatcgg caatgaccta actgtaacca tggcagccga aagcgggcaa ttgcagctca acgcgttcga gccgctgatc gtctacaata tcctcagttc catgcgactg ctcggccgcg ccatgaccaa tctggccgaa cgttgcgtcg acggaattga agccaatgtg gaacgctgtc gcgcaggcgc ggaggaaagc atttcgcttg caaccgccct cgtcccggtc gtgggctatg ccagggcggc agagatcgcc aagcaggcgc ttgcctcagg gcagaccgtc atggaagtgg cgatttccaa ggggctggac gcatctgcgc tcacgataat gcttgacccc ttgagaatgg cattcccgcc ggaaacgcac gacaaggagg cacatcatgc atctgtcggc aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for BrabA.00047.a.A5.GE28033
| HARVESTED ON: | 12/7/2009 |
| SEQUENCED ON: | 12/23/2009 |
| EXPECTED MW: | 51kDa |
| OBSERVED MW: | 48kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 28 |
Validated AA Sequence
MPGKVNPVIP ESVNQVCYQV IGNDLTVTMA AESGQLQLNA FEPLIVYNIL SSMRLLGRAM TNLAERCVDG IEANVERCRA GAEESISLAT ALVPVVGYAR AAEIAKQALA SGQTVMEVAI SKGLDASALT IMLDPLRM
Validated NT Sequence
cnnnnttcgg gctttgttag cagccggatc ctcgagaagc ttggctgcag aacttgttcg tgctgtttat tacattctca aggggtcaag cattatcgtg agcgcagatg cgtccagccc cttggaaatc gccacttcca tgacggtctg ccctgaggca agcgcctgct tggcgatctc tgccgccctg gcatagccca cgaccgggac gagggcggtt gcaagcgaaa tgctttcctc cgcgcctgcg cgacagcgtt ccacattggc ttcaattccg tcgacgcaac gttcggccag attggtcatg gcgcggccga gcagtcgcat ggaactgagg atattgtaga cgatcagcgg ctcgaacgcg ttgagctgca attgcccgct ttcggctgcc atggttacag ttaggtcatt gccgatcacc tgatagcaga cctgattgac cgattccggt atgaccggat tgacctttcc cggcatgatc gacgatcccg gctgaaccgc aggcaggcgg atttcaccaa ggccgcttcg cggcccgctt gagagtagcc gcagatcgtt ggcgatcttg gaaagcttga ctgcgatccg gcgcaatatc ccggaaaagg tgacgaacgc accggtatcc cagctcgctt ccacgagatt gccggtggct ttcagttcga taccggaaat ctgcgaaagt tccacgatgg cctgttcggc ataggcgtgc gaggcgttga tgcgcgtgcc gatagcggtt ccaccgaggt ttacttcgcg gaaaagcgcg gcaacctcct ccagccgggc tgtgtcctcc cgcaacgttg cggcaaatgc ctcgaactcc tgtccaagag tgatcggaac cgcatcctgc aactgtgtgc gcccgatctt gatgaccgtc gcgaattccc gcccctttgc ttcaaaggcg gcaatcagcc ggtgcagcgc cgtctgaacc tggnnctggc tgananaanc gccagcctta ccgnggnngg ntacncatcn ttggnggnct gnnacatgnt gacgtcntca tnnggntggn tcgnnnggna nnnnccncnn nnnncccnnt cnnngnnnnn nnnnnnngan nncnncnntn nnntnnnant nnnnnnnnnn nnncnnannn nnncncnnnn nannnnnnnn nnnnncnnnn nnnnnnnnnn nnngnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnannnnn nnnngnnnnn nnnnnncncn nnnnnnt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTRREQDSL GERDIPMDAY FGIQTLRAVE NFSLSDVALN HIPALVRALA MVKKAAATAN YKLRQLPEPK YAAIVAACDD IIDGLLMEQF VVDVFQGGAG TSSNMNANEV IANRALEHLG RPRGDYQTIH PNDDVNMSQS TNDVYPTAVR LALLLSQNQV QTALHRLIAA FEAKGREFAT VIKIGRTQLQ DAVPITLGQE FEAFAATLRE DTARLEEVAA LFREVNLGGT AIGTRINASH AYAEQAIVEL SQISGIELKA TGNLVEASWD TGAFVTFSGI LRRIAVKLSK IANDLRLLSS GPRSGLGEIR LPAVQPGSSI MPGKVNPVIP ESVNQVCYQV IGNDLTVTMA AESGQLQLNA FEPLIVYNIL SSMRLLGRAM TNLAERCVDG IEANVERCRA GAEESISLAT ALVPVVGYAR AAEIAKQALA SGQTVMEVAI SKGLDASALT IMLDPLRM
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacacggcgc gagcaggatt cccttgggga gcgcgatatt cccatggatg cctatttcgg catccagacc ctacgggcag ttgaaaactt ctcgctttcc gatgttgcgc tgaaccatat tcctgccctc gttcgcgccc ttgcaatggt caagaaagcg gctgccaccg ccaattacaa gctgcggcag ctgccggagc ccaaatatgc ggccatcgtc gccgcctgcg acgacatcat tgacgggctt ttgatggagc aattcgtcgt ggatgtgttt cagggcggcg cgggcacctc aagcaatatg aacgcaaatg aggttatcgc aaaccgcgcc cttgaacatc tggggcggcc gcgaggcgac taccagacga tccatcccaa tgatgacgtc aacatgtcgc agtccaccaa tgatgtgtat ccaaccgcgg taaggctggc gcttcttctc agccagaacc aggttcagac ggcgctgcac cggctgattg ccgcctttga agcaaagggg cgggaattcg cgacggtcat caagatcggg cgcacacagt tgcaggatgc ggttccgatc actcttggac aggagttcga ggcatttgcc gcaacgttgc gggaggacac agcccggctg gaggaggttg ccgcgctttt ccgcgaagta aacctcggtg gaaccgctat cggcacgcgc atcaacgcct cgcacgccta tgccgaacag gccatcgtgg aactttcgca gatttccggt atcgaactga aagccaccgg caatctcgtg gaagcgagct gggataccgg tgcgttcgtc accttttccg ggatattgcg ccggatcgca gtcaagcttt ccaagatcgc caacgatctg cggctactct caagcgggcc gcgaagcggc cttggtgaaa tccgcctgcc tgcggttcag ccgggatcgt cgatcatgcc gggaaaggtc aatccggtca taccggaatc ggtcaatcag gtctgctatc aggtgatcgg caatgaccta actgtaacca tggcagccga aagcgggcaa ttgcagctca acgcgttcga gccgctgatc gtctacaata tcctcagttc catgcgactg ctcggccgcg ccatgaccaa tctggccgaa cgttgcgtcg acggaattga agccaatgtg gaacgctgtc gcgcaggcgc ggaggaaagc atttcgcttg caaccgccct cgtcccggtc gtgggctatg ccagggcggc agagatcgcc aagcaggcgc ttgcctcagg gcagaccgtc atggaagtgg cgatttccaa ggggctggac gcatctgcgc tcacgataat gcttgacccc ttgagaatga aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for BrabA.00047.a.A6.GE28034
| HARVESTED ON: | 12/7/2009 |
| SEQUENCED ON: | 12/23/2009 |
| EXPECTED MW: | 52kDa |
| OBSERVED MW: | 49kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with sequence variation |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTRREQDSL GERDIPMDAY FGIQTLRAVE NFSLSDVALN HIPALVRALA MVKKAAATAN YKLRQLPEPK YAAIVAACDD IIDGLLMEQF VVDVFQGGAG TSSNMNANEV IANRALEHLG RPRGDYQTIH PNDDVNMSQS TNDVYPTAVR LALLLSQNQV QTALHRLIAA FEAKGREFAT VIKIGRTQLQ DAVPITLGQE FEAFAATLRE DTARLEEVAA LFREVNLGGT AIGTRINASH AYAEQAIVEL SQISGIELKA TGNLVEASWD TGAFVTFSGI LRRIAVKLSK IANDLRLLSS GPRSGLGEIR LPAVQPGSSI MPGKVNPVIP ESVNQVCYQV IGNDLTVTMA AESGQLQLNA FEPLIVYNIL SSMRLLGRAM TNLAERCVDG IEANVERCRA GAEESISLAT ALVPVVGYAR AAEIAKQALA SGQTVMEVAI SKGLDASALT IMLDPLRMAF PPETHDK
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacac ggcgcgagca ggattccctt ggggagcgcg atattcccat ggatgcctat ttcggcatcc agaccctacg ggcagttgaa aacttctcgc tttccgatgt tgcgctgaac catattcctg ccctcgttcg cgcccttgca atggtcaaga aagcggctgc caccgccaat tacaagctgc ggcagctgcc ggagcccaaa tatgcggcca tcgtcgccgc ctgcgacgac atcattgacg ggcttttgat ggagcaattc gtcgtggatg tgtttcaggg cggcgcgggc acctcaagca atatgaacgc aaatgaggtt atcgcaaacc gcgcccttga acatctgggg cggccgcgag gcgactacca gacgatccat cccaatgatg acgtcaacat gtcgcagtcc accaatgatg tgtatccaac cgcggtaagg ctggcgcttc ttctcagcca gaaccaggtt cagacggcgc tgcaccggct gattgccgcc tttgaagcaa aggggcggga attcgcgacg gtcatcaaga tcgggcgcac acagttgcag gatgcggttc cgatcactct tggacaggag ttcgaggcat ttgccgcaac gttgcgggag gacacagccc ggctggagga ggttgccgcg cttttccgcg aagtaaacct cggtggaacc gctatcggca cgcgcatcaa cgcctcgcac gcctatgccg aacaggccat cgtggaactt tcgcagattt ccggtatcga actgaaagcc accggcaatc tcgtggaagc gagctgggat accggtgcgt tcgtcacctt ttccgggata ttgcgccgga tcgcagtcaa gctttccaag atcgccaacg atctgcggct actctcaagc gggccgcgaa gcggccttgg tgaaatccgc ctgcctgcgg ttcagccggg atcgtcgatc atgccgggaa aggtcaatcc ggtcataccg gaatcggtca atcaggtctg ctatcaggtg atcggcaatg acctaactgt aaccatggca gccgaaagcg ggcaattgca gctcaacgcg ttcgagccgc tgatcgtcta caatatcctc agttccatgc gactgctcgg ccgcgccatg accaatctgg ccgaacgttg cgtcgacgga attgaagcca atgtggaacg ctgtcgcgca ggcgcggagg aaagcatttc gcttgcaacc gccctcgtcc cggtcgtggg ctatgccagg gcggcagaga tcgccaagca ggcgcttgcc tcagggcaga ccgtcatgga agtggcgatt tccaaggggc tggacgcatc tgcgctcacg ataatgcttg accccttgag aatggcattc ccgccggaaa cgcacgacaa gaag
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTRREQDSL GERDIPMDAY FGIQTLRAVE NFSLSDVALN HIPALVRALA MVKKAAATAN YKLRQLPEPK YAAIVAACDD IIDGLLMEQF VVDVFQGGAG TSSNMNANEV IANRALEHLG RPRGDYQTIH PNDDVNMSQS TNDVYPTAVR LALLLSQNQV QTALHRLIAA FEAKGREFAT VIKIGRTQLQ DAVPITLGQE FEAFAATLRE DTARLEEVAA LFREVNLGGT AIGTRINASH AYAEQAIVEL SQISGIELKA TGNLVEASWD TGAFVTFSGI LRRIAVKLSK IANDLRLLSS GPRSGLGEIR LPAVQPGSSI MPGKVNPVIP ESVNQVCYQV IGNDLTVTMA AESGQLQLNA FEPLIVYNIL SSMRLLGRAM TNLAERCVDG IEANVERCRA GAEESISLAT ALVPVVGYAR AAEIAKQALA SGQTVMEVAI SKGLDASALT IMLDPLRMAF PPETHDKEAH H
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacacggcgc gagcaggatt cccttgggga gcgcgatatt cccatggatg cctatttcgg catccagacc ctacgggcag ttgaaaactt ctcgctttcc gatgttgcgc tgaaccatat tcctgccctc gttcgcgccc ttgcaatggt caagaaagcg gctgccaccg ccaattacaa gctgcggcag ctgccggagc ccaaatatgc ggccatcgtc gccgcctgcg acgacatcat tgacgggctt ttgatggagc aattcgtcgt ggatgtgttt cagggcggcg cgggcacctc aagcaatatg aacgcaaatg aggttatcgc aaaccgcgcc cttgaacatc tggggcggcc gcgaggcgac taccagacga tccatcccaa tgatgacgtc aacatgtcgc agtccaccaa tgatgtgtat ccaaccgcgg taaggctggc gcttcttctc agccagaacc aggttcagac ggcgctgcac cggctgattg ccgcctttga agcaaagggg cgggaattcg cgacggtcat caagatcggg cgcacacagt tgcaggatgc ggttccgatc actcttggac aggagttcga ggcatttgcc gcaacgttgc gggaggacac agcccggctg gaggaggttg ccgcgctttt ccgcgaagta aacctcggtg gaaccgctat cggcacgcgc atcaacgcct cgcacgccta tgccgaacag gccatcgtgg aactttcgca gatttccggt atcgaactga aagccaccgg caatctcgtg gaagcgagct gggataccgg tgcgttcgtc accttttccg ggatattgcg ccggatcgca gtcaagcttt ccaagatcgc caacgatctg cggctactct caagcgggcc gcgaagcggc cttggtgaaa tccgcctgcc tgcggttcag ccgggatcgt cgatcatgcc gggaaaggtc aatccggtca taccggaatc ggtcaatcag gtctgctatc aggtgatcgg caatgaccta actgtaacca tggcagccga aagcgggcaa ttgcagctca acgcgttcga gccgctgatc gtctacaata tcctcagttc catgcgactg ctcggccgcg ccatgaccaa tctggccgaa cgttgcgtcg acggaattga agccaatgtg gaacgctgtc gcgcaggcgc ggaggaaagc atttcgcttg caaccgccct cgtcccggtc gtgggctatg ccagggcggc agagatcgcc aagcaggcgc ttgcctcagg gcagaccgtc atggaagtgg cgatttccaa ggggctggac gcatctgcgc tcacgataat gcttgacccc ttgagaatgg cattcccgcc ggaaacgcac gacaaggagg cacatcataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for BrabA.00047.a.D19.PD00037
| PURIFICATION DATe: | 3/12/2009 |
| CONCENTRATION: | 33.6mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSMTT LENIHPLTRR EQDSLGERDI PMDAYFGIQT LRAVENFSLS DVALNHIPAL VRALAMVKKA AATANYKLRQ LPEPKYAAIV AACDDIIDGL LMEQFVVDVF QGGAGTSSNM NANEVIANRA LEHLGRPRGD YQTIHPNDDV NMSQSTNDVY PTAVRLALLL SQNQVQTALH RLIAAFEAKG REFATVIKIG RTQLQDAVPI TLGQEFEAFA ATLREDTARL EEVAALFREV NLGGTAIGTR INASHAYAEQ AIVELSQISG IELKATGNLV EASWDTGAFV TFSGILRRIA VKLSKIANDL RLLSSGPRSG LGEIRLPAVQ PGSSIMPGKV NPVIPESVNQ VCYQVIGNDL TVTMAAESGQ LQLNAFEPLI VYNILSSMRL LGRAMTNLAE RCVDGIEANV ERCRAGAEES ISLATALVPV VGYARAAEIA KQALASGQTV MEVAISKGLD ASALTIMLDP LRMAFPPETH DKEAHHASVG
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCCACC ACCACCACCA CCATAGCGGC GAAGTAAAAC CGGAAGTGAA GCCGGAGACC CACATCAACC TGAAGGTTAG CGACGGTAGC AGCGAAATCT TCTTCAAGAT TAAGAAGACC ACCCCGCTGC GTCGCCTGAT GGAAGCATTC GCTAAACGCC AGGGCAAGGA AATGGATTCC CTGCGCTTTC TGTACGACGG TATCCGTATT CAGGCAGACC AGACTCCGGA AGATCTGGAC ATGGAAGATA ACGACATTAT CGAAGCACAC CGTGAACAAA TCGGTGGATC CATGACAACC CTGGAAAATA TTCATCCCCT GACACGGCGC GAGCAGGATT CCCTTGGGGA GCGCGATATT CCCATGGATG CCTATTTCGG CATCCAGACC CTACGGGCAG TTGAAAACTT CTCGCTTTCC GATGTTGCGC TGAACCATAT TCCTGCCCTC GTTCGCGCCC TTGCAATGGT CAAGAAAGCG GCTGCCACCG CCAATTACAA GCTGCGGCAG CTGCCGGAGC CCAAATATGC GGCCATCGTC GCCGCCTGCG ACGACATCAT TGACGGGCTT TTGATGGAGC AATTCGTCGT GGATGTGTTT CAGGGCGGCG CGGGCACCTC AAGCAATATG AACGCAAATG AGGTTATCGC AAACCGCGCC CTCGAGCATC TGGGGCGGCC GCGAGGCGAC TACCAGACGA TCCATCCCAA TGATGACGTC AACATGTCGC AGTCCACCAA TGATGTGTAT CCAACCGCGG TAAGGCTGGC GCTTCTTCTC AGCCAGAACC AGGTTCAGAC GGCGCTGCAC CGGCTGATTG CCGCCTTTGA AGCAAAGGGG CGGGAATTCG CGACGGTCAT CAAGATCGGG CGCACACAGT TGCAGGATGC GGTTCCGATC ACTCTTGGAC AGGAGTTCGA GGCATTTGCC GCAACGTTGC GGGAGGACAC AGCCCGGCTG GAGGAGGTTG CCGCGCTTTT CCGCGAAGTA AACCTCGGTG GAACCGCTAT CGGCACGCGC ATCAACGCCT CGCACGCCTA TGCCGAACAG GCCATCGTGG AACTTTCGCA GATTTCCGGT ATCGAACTGA AAGCCACCGG CAATCTCGTG GAAGCGAGCT GGGATACCGG TGCGTTCGTC ACCTTTTCCG GGATATTGCG CCGGATCGCA GTCAAGCTGT CCAAGATCGC CAACGATCTG CGGCTACTCT CAAGCGGGCC GCGAAGCGGC CTTGGTGAAA TCCGCCTGCC TGCGGTTCAG CCGGGATCGT CGATCATGCC GGGAAAGGTC AATCCGGTCA TACCGGAATC GGTCAATCAG GTCTGCTATC AGGTGATCGG CAATGACCTA ACTGTAACCA TGGCAGCCGA AAGCGGGCAA TTGCAGCTCA ACGCGTTCGA GCCGCTGATC GTCTACAATA TCCTCAGTTC CATGCGACTG CTCGGCCGCG CCATGACCAA TCTGGCCGAA CGTTGCGTCG ACGGAATTGA AGCCAATGTG GAACGCTGTC GCGCAGGCGC GGAGGAAAGC ATTTCGCTTG CAACCGCCCT CGTCCCGGTC GTGGGCTATG CCAGGGCGGC AGAGATCGCC AAGCAGGCGC TTGCCTCAGG GCAGACCGTC ATGGAAGTGG CGATTTCCAA GGGGCTGGAC GCATCTGCGC TCACGATAAT GCTTGACCCC TTGAGAATGG CATTCCCGCC GGAAACGCAC GACAAGGAGG CACATCATGC ATCTGTCGGC TAATAAAAGC TTGCGGCCGC ACTCGAGCAC CACCACCACC ACCACTGAGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGAT
Details for BrabA.00047.a.A4.PS00510
| PURIFICATION DATe: | 3/4/2010 |
| CONCENTRATION: | 25.88mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 21 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTRREQDSL GERDIPMDAY FGIQTLRAVE NFSLSDVALN HIPALVRALA MVKKAAATAN YKLRQLPEPK YAAIVAACDD IIDGLLMEQF VVDVFQGGAG TSSNMNANEV IANRALEHLG RPRGDYQTIH PNDDVNMSQS TNDVYPTAVR LALLLSQNQV QTALHRLIAA FEAKGREFAT VIKIGRTQLQ DAVPITLGQE FEAFAATLRE DTARLEEVAA LFREVNLGGT AIGTRINASH AYAEQAIVEL SQISGIELKA TGNLVEASWD TGAFVTFSGI LRRIAVKLSK IANDLRLLSS GPRSGLGEIR LPAVQPGSSI MPGKVNPVIP ESVNQVCYQV IGNDLTVTMA AESGQLQLNA FEPLIVYNIL SSMRLLGRAM TNLAERCVDG IEANVERCRA GAEESISLAT ALVPVVGYAR AAEIAKQALA SGQTVMEVAI SKGLDASALT IMLDPLRMAF PPETHDKEAH HASVG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACACGGCGC GAGCAGGATT CCCTTGGGGA GCGCGATATT CCCATGGATG CCTATTTCGG CATCCAGACC CTACGGGCAG TTGAAAACTT CTCGCTTTCC GATGTTGCGC TGAACCATAT TCCTGCCCTC GTTCGCGCCC TTGCAATGGT CAAGAAAGCG GCTGCCACCG CCAATTACAA GCTGCGGCAG CTGCCGGAGC CCAAATATGC GGCCATCGTC GCCGCCTGCG ACGACATCAT TGACGGGCTT TTGATGGAGC AATTCGTCGT GGATGTGTTT CAGGGCGGCG CGGGCACCTC AAGCAATATG AACGCAAATG AGGTTATCGC AAACCGCGCC CTTGAACATC TGGGGCGGCC GCGAGGCGAC TACCAGACGA TCCATCCCAA TGATGACGTC AACATGTCGC AGTCCACCAA TGATGTGTAT CCAACCGCGG TAAGGCTGGC GCTTCTTCTC AGCCAGAACC AGGTTCAGAC GGCGCTGCAC CGGCTGATTG CCGCCTTTGA AGCAAAGGGG CGGGAATTCG CGACGGTCAT CAAGATCGGG CGCACACAGT TGCAGGATGC GGTTCCGATC ACTCTTGGAC AGGAGTTCGA GGCATTTGCC GCAACGTTGC GGGAGGACAC AGCCCGGCTG GAGGAGGTTG CCGCGCTTTT CCGCGAAGTA AACCTCGGTG GAACCGCTAT CGGCACGCGC ATCAACGCCT CGCACGCCTA TGCCGAACAG GCCATCGTGG AACTTTCGCA GATTTCCGGT ATCGAACTGA AAGCCACCGG CAATCTCGTG GAAGCGAGCT GGGATACCGG TGCGTTCGTC ACCTTTTCCG GGATATTGCG CCGGATCGCA GTCAAGCTTT CCAAGATCGC CAACGATCTG CGGCTACTCT CAAGCGGGCC GCGAAGCGGC CTTGGTGAAA TCCGCCTGCC TGCGGTTCAG CCGGGATCGT CGATCATGCC GGGAAAGGTC AATCCGGTCA TACCGGAATC GGTCAATCAG GTCTGCTATC AGGTGATCGG CAATGACCTA ACTGTAACCA TGGCAGCCGA AAGCGGGCAA TTGCAGCTCA ACGCGTTCGA GCCGCTGATC GTCTACAATA TCCTCAGTTC CATGCGACTG CTCGGCCGCG CCATGACCAA TCTGGCCGAA CGTTGCGTCG ACGGAATTGA AGCCAATGTG GAACGCTGTC GCGCAGGCGC GGAGGAAAGC ATTTCGCTTG CAACCGCCCT CGTCCCGGTC GTGGGCTATG CCAGGGCGGC AGAGATCGCC AAGCAGGCGC TTGCCTCAGG GCAGACCGTC ATGGAAGTGG CGATTTCCAA GGGGCTGGAC GCATCTGCGC TCACGATAAT GCTTGACCCC TTGAGAATGG CATTCCCGCC GGAAACGCAC GACAAGGAGG CACATCATGC ATCTGTCGGC AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA
Details for BrabA.00047.a.A5.PS00511
| PURIFICATION DATe: | 3/4/2010 |
| CONCENTRATION: | 86.6mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 28 |
Protocol Notes
notes unavailable
Validated AA Sequence
MPGKVNPVIP ESVNQVCYQV IGNDLTVTMA AESGQLQLNA FEPLIVYNIL SSMRLLGRAM TNLAERCVDG IEANVERCRA GAEESISLAT ALVPVVGYAR AAEIAKQALA SGQTVMEVAI SKGLDASALT IMLDPLRM
Validated NT Sequence
cnnnnttcgg gctttgttag cagccggatc ctcgagaagc ttggctgcag aacttgttcg tgctgtttat tacattctca aggggtcaag cattatcgtg agcgcagatg cgtccagccc cttggaaatc gccacttcca tgacggtctg ccctgaggca agcgcctgct tggcgatctc tgccgccctg gcatagccca cgaccgggac gagggcggtt gcaagcgaaa tgctttcctc cgcgcctgcg cgacagcgtt ccacattggc ttcaattccg tcgacgcaac gttcggccag attggtcatg gcgcggccga gcagtcgcat ggaactgagg atattgtaga cgatcagcgg ctcgaacgcg ttgagctgca attgcccgct ttcggctgcc atggttacag ttaggtcatt gccgatcacc tgatagcaga cctgattgac cgattccggt atgaccggat tgacctttcc cggcatgatc gacgatcccg gctgaaccgc aggcaggcgg atttcaccaa ggccgcttcg cggcccgctt gagagtagcc gcagatcgtt ggcgatcttg gaaagcttga ctgcgatccg gcgcaatatc ccggaaaagg tgacgaacgc accggtatcc cagctcgctt ccacgagatt gccggtggct ttcagttcga taccggaaat ctgcgaaagt tccacgatgg cctgttcggc ataggcgtgc gaggcgttga tgcgcgtgcc gatagcggtt ccaccgaggt ttacttcgcg gaaaagcgcg gcaacctcct ccagccgggc tgtgtcctcc cgcaacgttg cggcaaatgc ctcgaactcc tgtccaagag tgatcggaac cgcatcctgc aactgtgtgc gcccgatctt gatgaccgtc gcgaattccc gcccctttgc ttcaaaggcg gcaatcagcc ggtgcagcgc cgtctgaacc tggnnctggc tgananaanc gccagcctta ccgnggnngg ntacncatcn ttggnggnct gnnacatgnt gacgtcntca tnnggntggn tcgnnnggna nnnnccncnn nnnncccnnt cnnngnnnnn nnnnnnngan nncnncnntn nnntnnnant nnnnnnnnnn nnncnnannn nnncncnnnn nannnnnnnn nnnnncnnnn nnnnnnnnnn nnngnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnannnnn nnnngnnnnn nnnnnncncn nnnnnnt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTRREQDSL GERDIPMDAY FGIQTLRAVE NFSLSDVALN HIPALVRALA MVKKAAATAN YKLRQLPEPK YAAIVAACDD IIDGLLMEQF VVDVFQGGAG TSSNMNANEV IANRALEHLG RPRGDYQTIH PNDDVNMSQS TNDVYPTAVR LALLLSQNQV QTALHRLIAA FEAKGREFAT VIKIGRTQLQ DAVPITLGQE FEAFAATLRE DTARLEEVAA LFREVNLGGT AIGTRINASH AYAEQAIVEL SQISGIELKA TGNLVEASWD TGAFVTFSGI LRRIAVKLSK IANDLRLLSS GPRSGLGEIR LPAVQPGSSI MPGKVNPVIP ESVNQVCYQV IGNDLTVTMA AESGQLQLNA FEPLIVYNIL SSMRLLGRAM TNLAERCVDG IEANVERCRA GAEESISLAT ALVPVVGYAR AAEIAKQALA SGQTVMEVAI SKGLDASALT IMLDPLRM
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACACGGCGC GAGCAGGATT CCCTTGGGGA GCGCGATATT CCCATGGATG CCTATTTCGG CATCCAGACC CTACGGGCAG TTGAAAACTT CTCGCTTTCC GATGTTGCGC TGAACCATAT TCCTGCCCTC GTTCGCGCCC TTGCAATGGT CAAGAAAGCG GCTGCCACCG CCAATTACAA GCTGCGGCAG CTGCCGGAGC CCAAATATGC GGCCATCGTC GCCGCCTGCG ACGACATCAT TGACGGGCTT TTGATGGAGC AATTCGTCGT GGATGTGTTT CAGGGCGGCG CGGGCACCTC AAGCAATATG AACGCAAATG AGGTTATCGC AAACCGCGCC CTTGAACATC TGGGGCGGCC GCGAGGCGAC TACCAGACGA TCCATCCCAA TGATGACGTC AACATGTCGC AGTCCACCAA TGATGTGTAT CCAACCGCGG TAAGGCTGGC GCTTCTTCTC AGCCAGAACC AGGTTCAGAC GGCGCTGCAC CGGCTGATTG CCGCCTTTGA AGCAAAGGGG CGGGAATTCG CGACGGTCAT CAAGATCGGG CGCACACAGT TGCAGGATGC GGTTCCGATC ACTCTTGGAC AGGAGTTCGA GGCATTTGCC GCAACGTTGC GGGAGGACAC AGCCCGGCTG GAGGAGGTTG CCGCGCTTTT CCGCGAAGTA AACCTCGGTG GAACCGCTAT CGGCACGCGC ATCAACGCCT CGCACGCCTA TGCCGAACAG GCCATCGTGG AACTTTCGCA GATTTCCGGT ATCGAACTGA AAGCCACCGG CAATCTCGTG GAAGCGAGCT GGGATACCGG TGCGTTCGTC ACCTTTTCCG GGATATTGCG CCGGATCGCA GTCAAGCTTT CCAAGATCGC CAACGATCTG CGGCTACTCT CAAGCGGGCC GCGAAGCGGC CTTGGTGAAA TCCGCCTGCC TGCGGTTCAG CCGGGATCGT CGATCATGCC GGGAAAGGTC AATCCGGTCA TACCGGAATC GGTCAATCAG GTCTGCTATC AGGTGATCGG CAATGACCTA ACTGTAACCA TGGCAGCCGA AAGCGGGCAA TTGCAGCTCA ACGCGTTCGA GCCGCTGATC GTCTACAATA TCCTCAGTTC CATGCGACTG CTCGGCCGCG CCATGACCAA TCTGGCCGAA CGTTGCGTCG ACGGAATTGA AGCCAATGTG GAACGCTGTC GCGCAGGCGC GGAGGAAAGC ATTTCGCTTG CAACCGCCCT CGTCCCGGTC GTGGGCTATG CCAGGGCGGC AGAGATCGCC AAGCAGGCGC TTGCCTCAGG GCAGACCGTC ATGGAAGTGG CGATTTCCAA GGGGCTGGAC GCATCTGCGC TCACGATAAT GCTTGACCCC TTGAGAATGA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A
Details for BrabA.00047.a.A6.PS00513
| PURIFICATION DATe: | 3/10/2010 |
| CONCENTRATION: | 46.3mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTRREQDSL GERDIPMDAY FGIQTLRAVE NFSLSDVALN HIPALVRALA MVKKAAATAN YKLRQLPEPK YAAIVAACDD IIDGLLMEQF VVDVFQGGAG TSSNMNANEV IANRALEHLG RPRGDYQTIH PNDDVNMSQS TNDVYPTAVR LALLLSQNQV QTALHRLIAA FEAKGREFAT VIKIGRTQLQ DAVPITLGQE FEAFAATLRE DTARLEEVAA LFREVNLGGT AIGTRINASH AYAEQAIVEL SQISGIELKA TGNLVEASWD TGAFVTFSGI LRRIAVKLSK IANDLRLLSS GPRSGLGEIR LPAVQPGSSI MPGKVNPVIP ESVNQVCYQV IGNDLTVTMA AESGQLQLNA FEPLIVYNIL SSMRLLGRAM TNLAERCVDG IEANVERCRA GAEESISLAT ALVPVVGYAR AAEIAKQALA SGQTVMEVAI SKGLDASALT IMLDPLRMAF PPETHDK
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacac ggcgcgagca ggattccctt ggggagcgcg atattcccat ggatgcctat ttcggcatcc agaccctacg ggcagttgaa aacttctcgc tttccgatgt tgcgctgaac catattcctg ccctcgttcg cgcccttgca atggtcaaga aagcggctgc caccgccaat tacaagctgc ggcagctgcc ggagcccaaa tatgcggcca tcgtcgccgc ctgcgacgac atcattgacg ggcttttgat ggagcaattc gtcgtggatg tgtttcaggg cggcgcgggc acctcaagca atatgaacgc aaatgaggtt atcgcaaacc gcgcccttga acatctgggg cggccgcgag gcgactacca gacgatccat cccaatgatg acgtcaacat gtcgcagtcc accaatgatg tgtatccaac cgcggtaagg ctggcgcttc ttctcagcca gaaccaggtt cagacggcgc tgcaccggct gattgccgcc tttgaagcaa aggggcggga attcgcgacg gtcatcaaga tcgggcgcac acagttgcag gatgcggttc cgatcactct tggacaggag ttcgaggcat ttgccgcaac gttgcgggag gacacagccc ggctggagga ggttgccgcg cttttccgcg aagtaaacct cggtggaacc gctatcggca cgcgcatcaa cgcctcgcac gcctatgccg aacaggccat cgtggaactt tcgcagattt ccggtatcga actgaaagcc accggcaatc tcgtggaagc gagctgggat accggtgcgt tcgtcacctt ttccgggata ttgcgccgga tcgcagtcaa gctttccaag atcgccaacg atctgcggct actctcaagc gggccgcgaa gcggccttgg tgaaatccgc ctgcctgcgg ttcagccggg atcgtcgatc atgccgggaa aggtcaatcc ggtcataccg gaatcggtca atcaggtctg ctatcaggtg atcggcaatg acctaactgt aaccatggca gccgaaagcg ggcaattgca gctcaacgcg ttcgagccgc tgatcgtcta caatatcctc agttccatgc gactgctcgg ccgcgccatg accaatctgg ccgaacgttg cgtcgacgga attgaagcca atgtggaacg ctgtcgcgca ggcgcggagg aaagcatttc gcttgcaacc gccctcgtcc cggtcgtggg ctatgccagg gcggcagaga tcgccaagca ggcgcttgcc tcagggcaga ccgtcatgga agtggcgatt tccaaggggc tggacgcatc tgcgctcacg ataatgcttg accccttgag aatggcattc ccgccggaaa cgcacgacaa gaag
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTRREQDSL GERDIPMDAY FGIQTLRAVE NFSLSDVALN HIPALVRALA MVKKAAATAN YKLRQLPEPK YAAIVAACDD IIDGLLMEQF VVDVFQGGAG TSSNMNANEV IANRALEHLG RPRGDYQTIH PNDDVNMSQS TNDVYPTAVR LALLLSQNQV QTALHRLIAA FEAKGREFAT VIKIGRTQLQ DAVPITLGQE FEAFAATLRE DTARLEEVAA LFREVNLGGT AIGTRINASH AYAEQAIVEL SQISGIELKA TGNLVEASWD TGAFVTFSGI LRRIAVKLSK IANDLRLLSS GPRSGLGEIR LPAVQPGSSI MPGKVNPVIP ESVNQVCYQV IGNDLTVTMA AESGQLQLNA FEPLIVYNIL SSMRLLGRAM TNLAERCVDG IEANVERCRA GAEESISLAT ALVPVVGYAR AAEIAKQALA SGQTVMEVAI SKGLDASALT IMLDPLRMAF PPETHDKEAH H
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACACGGCGC GAGCAGGATT CCCTTGGGGA GCGCGATATT CCCATGGATG CCTATTTCGG CATCCAGACC CTACGGGCAG TTGAAAACTT CTCGCTTTCC GATGTTGCGC TGAACCATAT TCCTGCCCTC GTTCGCGCCC TTGCAATGGT CAAGAAAGCG GCTGCCACCG CCAATTACAA GCTGCGGCAG CTGCCGGAGC CCAAATATGC GGCCATCGTC GCCGCCTGCG ACGACATCAT TGACGGGCTT TTGATGGAGC AATTCGTCGT GGATGTGTTT CAGGGCGGCG CGGGCACCTC AAGCAATATG AACGCAAATG AGGTTATCGC AAACCGCGCC CTTGAACATC TGGGGCGGCC GCGAGGCGAC TACCAGACGA TCCATCCCAA TGATGACGTC AACATGTCGC AGTCCACCAA TGATGTGTAT CCAACCGCGG TAAGGCTGGC GCTTCTTCTC AGCCAGAACC AGGTTCAGAC GGCGCTGCAC CGGCTGATTG CCGCCTTTGA AGCAAAGGGG CGGGAATTCG CGACGGTCAT CAAGATCGGG CGCACACAGT TGCAGGATGC GGTTCCGATC ACTCTTGGAC AGGAGTTCGA GGCATTTGCC GCAACGTTGC GGGAGGACAC AGCCCGGCTG GAGGAGGTTG CCGCGCTTTT CCGCGAAGTA AACCTCGGTG GAACCGCTAT CGGCACGCGC ATCAACGCCT CGCACGCCTA TGCCGAACAG GCCATCGTGG AACTTTCGCA GATTTCCGGT ATCGAACTGA AAGCCACCGG CAATCTCGTG GAAGCGAGCT GGGATACCGG TGCGTTCGTC ACCTTTTCCG GGATATTGCG CCGGATCGCA GTCAAGCTTT CCAAGATCGC CAACGATCTG CGGCTACTCT CAAGCGGGCC GCGAAGCGGC CTTGGTGAAA TCCGCCTGCC TGCGGTTCAG CCGGGATCGT CGATCATGCC GGGAAAGGTC AATCCGGTCA TACCGGAATC GGTCAATCAG GTCTGCTATC AGGTGATCGG CAATGACCTA ACTGTAACCA TGGCAGCCGA AAGCGGGCAA TTGCAGCTCA ACGCGTTCGA GCCGCTGATC GTCTACAATA TCCTCAGTTC CATGCGACTG CTCGGCCGCG CCATGACCAA TCTGGCCGAA CGTTGCGTCG ACGGAATTGA AGCCAATGTG GAACGCTGTC GCGCAGGCGC GGAGGAAAGC ATTTCGCTTG CAACCGCCCT CGTCCCGGTC GTGGGCTATG CCAGGGCGGC AGAGATCGCC AAGCAGGCGC TTGCCTCAGG GCAGACCGTC ATGGAAGTGG CGATTTCCAA GGGGCTGGAC GCATCTGCGC TCACGATAAT GCTTGACCCC TTGAGAATGG CATTCCCGCC GGAAACGCAC GACAAGGAGG CACATCATAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA
Details for BrabA.00047.a.A3.PS00519
| PURIFICATION DATe: | 3/10/2010 |
| CONCENTRATION: | 44.1mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 76 |
| PERCENT COVERAGE: | 80 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTTLENIHP LTRREQDSLG ERDIPMDAYF GIQTLRAVEN FSLSDVALNH IPALVRALAM VKKAAATANY KLRQLPEPKY AAIVAACDDI IDGLLMEQFV VDVFQGGAGT SSNMNANEVI ANRALEHLGR PRGDYQTIHP NDDVNMSQST NDVYPTAVRL ALLLSQNQVQ TALHRLIAAF EAKGREFATV IKIGRTQLQD AVPITLGQEF EAFAATLRXD TARLEEVAAL FREVNLGGTA IGTRINASHA YAEQAIVELS QISGIELKAT GXLVXRAGIP VRSSPFPGYC AGSQSSFXRS PTICGXSQAG XEXXLVKSAX LXXXXXRXSX XXXXSXXXXX XXXXXXXXXS XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX X
Validated NT Sequence
annnnnttnn nttnacttta agaaggagan ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacaaccctg gaaaatattc atcccctgac acggcgcgag caggattccc ttggggagcg cgatattccc atggatgcct atttcggcat ccagacccta cgggcagttg aaaacttctc gctttccgat gttgcgctga accatattcc tgccctcgtt cgcgcccttg caatggtcaa gaaagcggct gccaccgcca attacaagct gcggcagctg ccggagccca aatatgcggc catcgtcgcc gcctgcgacg acatcattga cgggcttttg atggagcaat tcgtcgtgga tgtgtttcag ggcggcgcgg gcacctcaag caatatgaac gcaaatgagg ttatcgcaaa ccgcgccctt gaacatctgg ggcggccgcg aggcgactac cagacgatcc atcccaatga tgacgtcaac atgtcgcagt ccaccaatga tgtgtatcca accgcggtaa ggctggcgct tcttctcagc cagaaccagg ttcagacggc gctgcaccgg ctgattgccg cctttgaagc aaaggggcgg gaattcgcga cggtcatcaa gatcgggcgc acacagttgc aggatgcggt tccgatcact cttggacagg agttcgaggc atttgccgca acgttgcggg angacacagc ccggctggag gaggttgccg cgcttttccg cgaagtaaac ctcggtggaa ccgctatcgg cacgcgcatc aacgcctcgc acgcctatgc cgaacaggcc atcgtggaac tttcgcagat ttccggtatc gaactgaaag ccaccggcan tctcgtnnng cgagctggga taccggtgcg ttcgtcacct tttccgggat attgcgccgg atcgcagtca agctttncaa gatcgccaac gatctgcggc tantctcaag cgggcnncga ancnnnnttg gtgaaatccg ccnnnctnnn gntcanccng gnncgtcnat catgnnnnna angncnatcc ngtnnnancn gnannngtca ncnngnnnnn nnnnnnngnn gatcngnnan nnanntnnnn nnnnnntgnn nnnnnnnnnn ngnnnannnn nnnnnnnnnn nnnnnntnnn nnnnnncnnt tnnnnnnnnn nnnnnnnnna
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTTLENIHP LTRREQDSLG ERDIPMDAYF GIQTLRAVEN FSLSDVALNH IPALVRALAM VKKAAATANY KLRQLPEPKY AAIVAACDDI IDGLLMEQFV VDVFQGGAGT SSNMNANEVI ANRALEHLGR PRGDYQTIHP NDDVNMSQST NDVYPTAVRL ALLLSQNQVQ TALHRLIAAF EAKGREFATV IKIGRTQLQD AVPITLGQEF EAFAATLRED TARLEEVAAL FREVNLGGTA IGTRINASHA YAEQAIVELS QISGIELKAT GNLVEASWDT GAFVTFSGIL RRIAVKLSKI ANDLRLLSSG PRSGLGEIRL PAVQPGSSIM PGKVNPVIPE SVNQVCYQVI GNDLTVTMAA ESGQLQLNAF EPLIVYNILS SMRLLGRAMT NLAERCVDGI EANVERCRAG AEESISLATA LVPVVGYARA AEIAKQALAS GQTVMEVAIS KGLDASALTI MLDPLRMAFP PETHDKEAHH
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACAACCCTG GAAAATATTC ATCCCCTGAC ACGGCGCGAG CAGGATTCCC TTGGGGAGCG CGATATTCCC ATGGATGCCT ATTTCGGCAT CCAGACCCTA CGGGCAGTTG AAAACTTCTC GCTTTCCGAT GTTGCGCTGA ACCATATTCC TGCCCTCGTT CGCGCCCTTG CAATGGTCAA GAAAGCGGCT GCCACCGCCA ATTACAAGCT GCGGCAGCTG CCGGAGCCCA AATATGCGGC CATCGTCGCC GCCTGCGACG ACATCATTGA CGGGCTTTTG ATGGAGCAAT TCGTCGTGGA TGTGTTTCAG GGCGGCGCGG GCACCTCAAG CAATATGAAC GCAAATGAGG TTATCGCAAA CCGCGCCCTT GAACATCTGG GGCGGCCGCG AGGCGACTAC CAGACGATCC ATCCCAATGA TGACGTCAAC ATGTCGCAGT CCACCAATGA TGTGTATCCA ACCGCGGTAA GGCTGGCGCT TCTTCTCAGC CAGAACCAGG TTCAGACGGC GCTGCACCGG CTGATTGCCG CCTTTGAAGC AAAGGGGCGG GAATTCGCGA CGGTCATCAA GATCGGGCGC ACACAGTTGC AGGATGCGGT TCCGATCACT CTTGGACAGG AGTTCGAGGC ATTTGCCGCA ACGTTGCGGG AGGACACAGC CCGGCTGGAG GAGGTTGCCG CGCTTTTCCG CGAAGTAAAC CTCGGTGGAA CCGCTATCGG CACGCGCATC AACGCCTCGC ACGCCTATGC CGAACAGGCC ATCGTGGAAC TTTCGCAGAT TTCCGGTATC GAACTGAAAG CCACCGGCAA TCTCGTGGAA GCGAGCTGGG ATACCGGTGC GTTCGTCACC TTTTCCGGGA TATTGCGCCG GATCGCAGTC AAGCTTTCCA AGATCGCCAA CGATCTGCGG CTACTCTCAA GCGGGCCGCG AAGCGGCCTT GGTGAAATCC GCCTGCCTGC GGTTCAGCCG GGATCGTCGA TCATGCCGGG AAAGGTCAAT CCGGTCATAC CGGAATCGGT CAATCAGGTC TGCTATCAGG TGATCGGCAA TGACCTAACT GTAACCATGG CAGCCGAAAG CGGGCAATTG CAGCTCAACG CGTTCGAGCC GCTGATCGTC TACAATATCC TCAGTTCCAT GCGACTGCTC GGCCGCGCCA TGACCAATCT GGCCGAACGT TGCGTCGACG GAATTGAAGC CAATGTGGAA CGCTGTCGCG CAGGCGCGGA GGAAAGCATT TCGCTTGCAA CCGCCCTCGT CCCGGTCGTG GGCTATGCCA GGGCGGCAGA GATCGCCAAG CAGGCGCTTG CCTCAGGGCA GACCGTCATG GAAGTGGCGA TTTCCAAGGG GCTGGACGCA TCTGCGCTCA CGATAATGCT TGACCCCTTG AGAATGGCAT TCCCGCCGGA AACGCACGAC AAGGAGGCAC ATCATAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA
Details for BrabA.00047.a.A2.PS00524
| PURIFICATION DATe: | 3/5/2010 |
| CONCENTRATION: | 79.59mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTTLENIHP LTRREQDSLG ERDIPMDAYF GIQTLRAVEN FSLSDVALNH IPALVRALAM VKKAAATANY KLRQLPEPKY AAIVAACDDI IDGLLMEQFV VDVFQGGAGT SSNMNANEVI ANRALEHLGR PRGDYQTIHP NDDVNMSQST NDVYPTAVRL ALLLSQNQVQ TALHRLIAAF EAKGREFATV IKIGRTQLQD AVPITLGQEF EAFAATLRED TARLEEVAAL FREVNLGGTA IGTRINASHA YAEQAIVELS QISGIELKAT GNLVEASWDT GAFVTFSGIL RRIAVKLSKI ANDLRLLSSG PRSGLGEIRL PAVQPGSSIM PGKVNPVIPE SVNQVCYQVI GNDLTVTMAA ESGQLQLNAF EPLIVYNILS SMRLLGRAMT NLAERCVDGI EANVERCRAG AEESISLATA LVPVVGYARA AEIAKQALAS GQTVMEVAIS KGLDASALTI MLDPLRM
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACAACCCTG GAAAATATTC ATCCCCTGAC ACGGCGCGAG CAGGATTCCC TTGGGGAGCG CGATATTCCC ATGGATGCCT ATTTCGGCAT CCAGACCCTA CGGGCAGTTG AAAACTTCTC GCTTTCCGAT GTTGCGCTGA ACCATATTCC TGCCCTCGTT CGCGCCCTTG CAATGGTCAA GAAAGCGGCT GCCACCGCCA ATTACAAGCT GCGGCAGCTG CCGGAGCCCA AATATGCGGC CATCGTCGCC GCCTGCGACG ACATCATTGA CGGGCTTTTG ATGGAGCAAT TCGTCGTGGA TGTGTTTCAG GGCGGCGCGG GCACCTCAAG CAATATGAAC GCAAATGAGG TTATCGCAAA CCGCGCCCTT GAACATCTGG GGCGGCCGCG AGGCGACTAC CAGACGATCC ATCCCAATGA TGACGTCAAC ATGTCGCAGT CCACCAATGA TGTGTATCCA ACCGCGGTAA GGCTGGCGCT TCTTCTCAGC CAGAACCAGG TTCAGACGGC GCTGCACCGG CTGATTGCCG CCTTTGAAGC AAAGGGGCGG GAATTCGCGA CGGTCATCAA GATCGGGCGC ACACAGTTGC AGGATGCGGT TCCGATCACT CTTGGACAGG AGTTCGAGGC ATTTGCCGCA ACGTTGCGGG AGGACACAGC CCGGCTGGAG GAGGTTGCCG CGCTTTTCCG CGAAGTAAAC CTCGGTGGAA CCGCTATCGG CACGCGCATC AACGCCTCGC ACGCCTATGC CGAACAGGCC ATCGTGGAAC TTTCGCAGAT TTCCGGTATC GAACTGAAAG CCACCGGCAA TCTCGTGGAA GCGAGCTGGG ATACCGGTGC GTTCGTCACC TTTTCCGGGA TATTGCGCCG GATCGCAGTC AAGCTTTCCA AGATCGCCAA CGATCTGCGG CTACTCTCAA GCGGGCCGCG AAGCGGCCTT GGTGAAATCC GCCTGCCTGC GGTTCAGCCG GGATCGTCGA TCATGCCGGG AAAGGTCAAT CCGGTCATAC CGGAATCGGT CAATCAGGTC TGCTATCAGG TGATCGGCAA TGACCTAACT GTAACCATGG CAGCCGAAAG CGGGCAATTG CAGCTCAACG CGTTCGAGCC GCTGATCGTC TACAATATCC TCAGTTCCAT GCGACTGCTC GGCCGCGCCA TGACCAATCT GGCCGAACGT TGCGTCGACG GAATTGAAGC CAATGTGGAA CGCTGTCGCG CAGGCGCGGA GGAAAGCATT TCGCTTGCAA CCGCCCTCGT CCCGGTCGTG GGCTATGCCA GGGCGGCAGA GATCGCCAAG CAGGCGCTTG CCTCAGGGCA GACCGTCATG GAAGTGGCGA TTTCCAAGGG GCTGGACGCA TCTGCGCTCA CGATAATGCT TGACCCCTTG AGAATGAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA