BrabA.00044.a
Zinc-containing alcohol dehydrogenase superfamily:Zinc-containing alcohol dehydrogenase
| CENTER ID: | BrabA.00044.a |
| ORGANISM: | Brucella melitensis biovar Abortus 2308 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.00044.a.A1.GE25380 | Full length( BrabA.00044.a ) | 1 | 344 | ||
| BrabA.00044.a.B1.GE25476 | Full length( BrabA.00044.a ) | 1 | 344 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.00044.a.A1.PW25418 | Full length( BrabA.00044.a ) | 1 | 344 | ||
| BrabA.00044.a.B1.PW25519 | Full length( BrabA.00044.a ) | 1 | 344 |
Structures
3MEQ
DEPOSITED: 3/31/2010
DETERMINATION: XRay
CLONE: BrabA.00044.a.B1.GE25476
PROTEIN: BrabA.00044.a.B1.PW25519
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126661 |
| PATRIC ID: | fig|359391.4.peg.213 |
| RefSeq: | YP_413690 |
| UniProt: | Q2YP66 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAKTMKAAVV RAFGKPLTID EVPIPQPGPG QIQVAIQASG VCHTDLHAAE GDWPVKPNPP FIPGHEGVGF VSAVGSGVKH VKEGDRVGIP WLYTACGHCR HCLGGWETLC EEQLNTGYSV NGGFAEYVVA DPNFVGHLPK NIDFNEIAPV LCAGVTVYKG LKVTDTKPGD WVVISGIGGL GHMAVQYARA MGLNVAAVDI DDRKLDLARR LGATVTVNAK TVADPAAYIR KETDGGAQGV LVTAVSPKAF EQALGMVARG GTVSLNGLPP GDFPLSIFNM VLNGVTVRGS IVGTRLDLQE SLDFAADGKV KATIQTGKLE DINAIFDDMR QGNIEGRIVM DLTQ
NT Sequence
ATGGCAAAGA CGATGAAAGC TGCGGTCGTG CGTGCCTTCG GCAAACCGCT GACGATTGAT GAAGTGCCGA TACCTCAACC CGGTCCCGGC CAGATTCAGG TTGCCATTCA GGCTTCCGGC GTATGCCATA CCGACCTTCA TGCCGCCGAG GGCGATTGGC CGGTAAAACC CAATCCGCCC TTCATTCCGG GTCATGAGGG TGTCGGTTTC GTTTCCGCCG TGGGCAGCGG CGTCAAGCAT GTCAAGGAAG GCGACCGTGT CGGCATTCCA TGGCTTTACA CTGCCTGCGG CCATTGCCGC CATTGCCTTG GTGGCTGGGA AACGCTTTGC GAGGAACAGC TCAATACCGG CTATTCCGTC AATGGCGGCT TTGCCGAATA TGTGGTGGCC GATCCGAATT TCGTCGGCCA CTTGCCGAAG AATATCGATT TCAACGAGAT TGCGCCGGTG CTCTGCGCGG GCGTGACGGT CTATAAGGGC CTCAAGGTCA CGGATACGAA GCCCGGTGAC TGGGTGGTCA TTTCAGGCAT AGGCGGGCTC GGCCACATGG CTGTGCAATA TGCCAGGGCC ATGGGGCTGA ATGTTGCCGC CGTCGATATT GACGACCGCA AGCTCGATCT CGCCCGCAGA CTTGGCGCAA CCGTTACGGT CAACGCTAAG ACGGTTGCCG ACCCGGCAGC CTATATTCGC AAGGAAACCG ATGGCGGTGC GCAAGGCGTG CTGGTGACGG CGGTTTCGCC CAAGGCTTTT GAACAGGCGC TGGGCATGGT TGCGCGCGGC GGTACTGTTT CGCTGAATGG CCTGCCGCCT GGCGATTTCC CGCTTTCCAT CTTCAACATG GTGCTCAATG GCGTAACGGT GCGCGGCTCC ATCGTGGGGA CGCGGCTTGA TTTGCAGGAA TCGCTCGATT TTGCAGCCGA CGGCAAGGTC AAGGCCACCA TCCAAACCGG CAAGCTGGAA GACATCAACG CCATCTTCGA CGATATGCGG CAAGGCAATA TCGAAGGCCG CATCGTGATG GACCTGACTC AA
Details for BrabA.00044.a.A1.GE25380
| HARVESTED ON: | 6/30/2008 |
| SEQUENCED ON: | 8/18/2010 |
| EXPECTED MW: | 38kDa |
| OBSERVED MW: | 39kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAKTMKAAV VRAFGKPLTI DEVPIPQPGP GQIQVAIQAS GVCHTDLHAA EGDWPVKPNP PFIPGHEGVG FVSAVGSGVK HVKEGDRVGI PWLYTACGHC RHCLGGWETL CEEQLNTGYS VNGGFAEYVV ADPNFVGHLP KNIDFNEIAP VLCAGVTVYK GLKVTDTKPG DWVVISGIGG LGHMAVQYAR AMGLNVAAVD IDDRKLDLAR RLGATVTVNA KTVADPAAYI RKETDGGAQG VLVTAVSPKA FEQALGMVAR GGTVSLNGLP PGDFPLSIFN MVLNGVTVRG SIVGTRLDLQ ESLDFAADGK VKATIQTGKL EDINAIFDDM RQGNIEGRIV MDLTQ
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcaa agacgatgaa agctgcggtc gtgcgtgcct tcggcaaacc gctgacgatt gatgaagtgc cgatacctca acccggtccc ggccagattc aggttgccat tcaggcttcc ggcgtatgcc ataccgacct tcatgccgcc gagggcgatt ggccggtaaa acccaatccg cccttcattc cgggtcatga gggtgtcggt ttcgtttccg ccgtgggcag cggcgtcaag catgtcaagg aaggcgaccg tgtcggcatt ccatggcttt acactgcctg cggccattgc cgccattgcc ttggtggctg ggaaacgctt tgcgaggaac agctcaatac cggctattcc gtcaatggcg gctttgccga atatgtggtg gccgatccga atttcgtcgg ccacttgccg aagaatatcg atttcaacga gattgcgccg gtgctctgcg cgggcgtgac ggtctataag ggcctcaagg tcacggatac gaagcccggt gactgggtgg tcatttcagg cataggcggg ctcggccaca tggctgtgca atatgccagg gccatggggc tgaatgttgc cgccgtcgat attgacgacc gcaagctcga tctcgcccgc agacttggcg caaccgttac ggtcaacgct aagacggttg ccgacccggc agcctatatt cgcaaggaaa ccgatggcgg tgcgcaaggc gtgctggtga cggcggtttc gcccaaggct tttgaacagg cgctgggcat ggttgcgcgc ggcggtactg tttcgctgaa tggcctgccg cctggcgatt tcccgctttc catcttcaac atggtgctca atggcgtaac ggtgcgcggc tccatcgtgg ggacgcggct tgatttgcag gaatcgctcg attttgcagc cgacggcaag gtcaaggcca ccatccaaac cggcaagctg gaagacatca acgccatctt cgacgatatg cggcaaggca atatcgaagg ccgcatcgtg atggacctga ctcaataata a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAKTMKAAV VRAFGKPLTI DEVPIPQPGP GQIQVAIQAS GVCHTDLHAA EGDWPVKPNP PFIPGHEGVG FVSAVGSGVK HVKEGDRVGI PWLYTACGHC RHCLGGWETL CEEQLNTGYS VNGGFAEYVV ADPNFVGHLP KNIDFNEIAP VLCAGVTVYK GLKVTDTKPG DWVVISGIGG LGHMAVQYAR AMGLNVAAVD IDDRKLDLAR RLGATVTVNA KTVADPAAYI RKETDGGAQG VLVTAVSPKA FEQALGMVAR GGTVSLNGLP PGDFPLSIFN MVLNGVTVRG SIVGTRLDLQ ESLDFAADGK VKATIQTGKL EDINAIFDDM RQGNIEGRIV MDLTQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcaaagacg atgaaagctg cggtcgtgcg tgccttcggc aaaccgctga cgattgatga agtgccgata cctcaacccg gtcccggcca gattcaggtt gccattcagg cttccggcgt atgccatacc gaccttcatg ccgccgaggg cgattggccg gtaaaaccca atccgccctt cattccgggt catgagggtg tcggtttcgt ttccgccgtg ggcagcggcg tcaagcatgt caaggaaggc gaccgtgtcg gcattccatg gctttacact gcctgcggcc attgccgcca ttgccttggt ggctgggaaa cgctttgcga ggaacagctc aataccggct attccgtcaa tggcggcttt gccgaatatg tggtggccga tccgaatttc gtcggccact tgccgaagaa tatcgatttc aacgagattg cgccggtgct ctgcgcgggc gtgacggtct ataagggcct caaggtcacg gatacgaagc ccggtgactg ggtggtcatt tcaggcatag gcgggctcgg ccacatggct gtgcaatatg ccagggccat ggggctgaat gttgccgccg tcgatattga cgaccgcaag ctcgatctcg cccgcagact tggcgcaacc gttacggtca acgctaagac ggttgccgac ccggcagcct atattcgcaa ggaaaccgat ggcggtgcgc aaggcgtgct ggtgacggcg gtttcgccca aggcttttga acaggcgctg ggcatggttg cgcgcggcgg tactgtttcg ctgaatggcc tgccgcctgg cgatttcccg ctttccatct tcaacatggt gctcaatggc gtaacggtgc gcggctccat cgtggggacg cggcttgatt tgcaggaatc gctcgatttt gcagccgacg gcaaggtcaa ggccaccatc caaaccggca agctggaaga catcaacgcc atcttcgacg atatgcggca aggcaatatc gaaggccgca tcgtgatgga cctgactcaa tagaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for BrabA.00044.a.B1.GE25476
| HARVESTED ON: | 7/11/2008 |
| SEQUENCED ON: | 7/28/2010 |
| EXPECTED MW: | 37kDa |
| OBSERVED MW: | 39kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMA KTMKAAVVRA FGKPLTIDEV PIPQPGPGQI QVAIQASGVC HTDLHAAEGD WPVKPNPPFI PGHEGVGFVS AVGSGVKHVK EGDRVGIPWL YTACGHCRHC LGGWETLCEE QLNTGYSVNG GFAEYVVADP NFVGHLPKNI DFNEIAPVLC AGVTVYKGLK VTDTKPGDWV VISGIGGLGH MAVQYARAMG LNVAAVDIDD RKLDLARRLG ATVTVNAKTV ADPAAYIRKE TDGGAQGVLV TAVSPKAFEQ ALGMVARGGT VSLNGLPPGD FPLSIFNMVL NGVTVRGSIV GTRLDLQESL DFAADGKVKA TIQTGKLEDI NAIFDDMRQG NIEGRIVMDL TQ
Validated NT Sequence
atggctcacc accaccacca ccatatggca aagacgatga aagctgcggt cgtgcgtgcc ttcggcaaac cgctgacgat tgatgaagtg ccgatacctc aacccggtcc cggccagatt caggttgcca ttcaggcttc cggcgtatgc cataccgacc ttcatgccgc cgagggcgat tggccggtaa aacccaatcc gcccttcatt ccgggtcatg agggtgtcgg tttcgtttcc gccgtgggca gcggcgtcaa gcatgtcaag gaaggcgacc gtgtcggcat tccatggctt tacactgcct gcggccattg ccgccattgc cttggtggct gggaaacgct ttgcgaggaa cagctcaata ccggctattc cgtcaatggc ggctttgccg aatatgtggt ggccgatccg aatttcgtcg gccacttgcc gaagaatatc gatttcaacg agattgcgcc ggtgctctgc gcgggcgtga cggtctataa gggcctcaag gtcacggata cgaagcccgg tgactgggtg gtcatttcag gcataggcgg gctcggccac atggctgtgc aatatgccag ggccatgggg ctgaatgttg ccgccgtcga tattgacgac cgcaagctcg atctcgcccg cagacttggc gcaaccgtta cggtcaacgc taagacggtt gccgacccgg cagcctatat tcgcaaggaa accgatggcg gtgcgcaagg cgtgctggtg acggcggttt cgcccaaggc ttttgaacag gcgctgggca tggttgcgcg cggcggtact gtttcgctga atggcctgcc gcctggcgat ttcccgcttt ccatcttcaa catggtgctc aatggcgtaa cggtgcgcgg ctccatcgtg gggacgcggc ttgatttgca ggaatcgctc gattttgcag ccgacggcaa ggtcaaggcc accatccaaa ccggcaagct ggaagacatc aacgccatct tcgacgatat gcggcaaggc aatatcgaag gccgcatcgt gatggacctg actcaataag tgagtaagat
Expected Protein Sequence
MAHHHHHHMA KTMKAAVVRA FGKPLTIDEV PIPQPGPGQI QVAIQASGVC HTDLHAAEGD WPVKPNPPFI PGHEGVGFVS AVGSGVKHVK EGDRVGIPWL YTACGHCRHC LGGWETLCEE QLNTGYSVNG GFAEYVVADP NFVGHLPKNI DFNEIAPVLC AGVTVYKGLK VTDTKPGDWV VISGIGGLGH MAVQYARAMG LNVAAVDIDD RKLDLARRLG ATVTVNAKTV ADPAAYIRKE TDGGAQGVLV TAVSPKAFEQ ALGMVARGGT VSLNGLPPGD FPLSIFNMVL NGVTVRGSIV GTRLDLQESL DFAADGKVKA TIQTGKLEDI NAIFDDMRQG NIEGRIVMDL TQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcaa agacgatgaa agctgcggtc gtgcgtgcct tcggcaaacc gctgacgatt gatgaagtgc cgatacctca acccggtccc ggccagattc aggttgccat tcaggcttcc ggcgtatgcc ataccgacct tcatgccgcc gagggcgatt ggccggtaaa acccaatccg cccttcattc cgggtcatga gggtgtcggt ttcgtttccg ccgtgggcag cggcgtcaag catgtcaagg aaggcgaccg tgtcggcatt ccatggcttt acactgcctg cggccattgc cgccattgcc ttggtggctg ggaaacgctt tgcgaggaac agctcaatac cggctattcc gtcaatggcg gctttgccga atatgtggtg gccgatccga atttcgtcgg ccacttgccg aagaatatcg atttcaacga gattgcgccg gtgctctgcg cgggcgtgac ggtctataag ggcctcaagg tcacggatac gaagcccggt gactgggtgg tcatttcagg cataggcggg ctcggccaca tggctgtgca atatgccagg gccatggggc tgaatgttgc cgccgtcgat attgacgacc gcaagctcga tctcgcccgc agacttggcg caaccgttac ggtcaacgct aagacggttg ccgacccggc agcctatatt cgcaaggaaa ccgatggcgg tgcgcaaggc gtgctggtga cggcggtttc gcccaaggct tttgaacagg cgctgggcat ggttgcgcgc ggcggtactg tttcgctgaa tggcctgccg cctggcgatt tcccgctttc catcttcaac atggtgctca atggcgtaac ggtgcgcggc tccatcgtgg ggacgcggct tgatttgcag gaatcgctcg attttgcagc cgacggcaag gtcaaggcca ccatccaaac cggcaagctg gaagacatca acgccatctt cgacgatatg cggcaaggca atatcgaagg ccgcatcgtg atggacctga ctcaatagtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for BrabA.00044.a.A1.PW25418
| PURIFICATION DATe: | 12/28/2009 |
| CONCENTRATION: | 21.11mg/ml |
| OBSERVED MW: | 37kDa |
| EXPRESSION LEVEL: | data unavailable |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAKTMKAAV VRAFGKPLTI DEVPIPQPGP GQIQVAIQAS GVCHTDLHAA EGDWPVKPNP PFIPGHEGVG FVSAVGSGVK HVKEGDRVGI PWLYTACGHC RHCLGGWETL CEEQLNTGYS VNGGFAEYVV ADPNFVGHLP KNIDFNEIAP VLCAGVTVYK GLKVTDTKPG DWVVISGIGG LGHMAVQYAR AMGLNVAAVD IDDRKLDLAR RLGATVTVNA KTVADPAAYI RKETDGGAQG VLVTAVSPKA FEQALGMVAR GGTVSLNGLP PGDFPLSIFN MVLNGVTVRG SIVGTRLDLQ ESLDFAADGK VKATIQTGKL EDINAIFDDM RQGNIEGRIV MDLTQ
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcaa agacgatgaa agctgcggtc gtgcgtgcct tcggcaaacc gctgacgatt gatgaagtgc cgatacctca acccggtccc ggccagattc aggttgccat tcaggcttcc ggcgtatgcc ataccgacct tcatgccgcc gagggcgatt ggccggtaaa acccaatccg cccttcattc cgggtcatga gggtgtcggt ttcgtttccg ccgtgggcag cggcgtcaag catgtcaagg aaggcgaccg tgtcggcatt ccatggcttt acactgcctg cggccattgc cgccattgcc ttggtggctg ggaaacgctt tgcgaggaac agctcaatac cggctattcc gtcaatggcg gctttgccga atatgtggtg gccgatccga atttcgtcgg ccacttgccg aagaatatcg atttcaacga gattgcgccg gtgctctgcg cgggcgtgac ggtctataag ggcctcaagg tcacggatac gaagcccggt gactgggtgg tcatttcagg cataggcggg ctcggccaca tggctgtgca atatgccagg gccatggggc tgaatgttgc cgccgtcgat attgacgacc gcaagctcga tctcgcccgc agacttggcg caaccgttac ggtcaacgct aagacggttg ccgacccggc agcctatatt cgcaaggaaa ccgatggcgg tgcgcaaggc gtgctggtga cggcggtttc gcccaaggct tttgaacagg cgctgggcat ggttgcgcgc ggcggtactg tttcgctgaa tggcctgccg cctggcgatt tcccgctttc catcttcaac atggtgctca atggcgtaac ggtgcgcggc tccatcgtgg ggacgcggct tgatttgcag gaatcgctcg attttgcagc cgacggcaag gtcaaggcca ccatccaaac cggcaagctg gaagacatca acgccatctt cgacgatatg cggcaaggca atatcgaagg ccgcatcgtg atggacctga ctcaataata a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAKTMKAAV VRAFGKPLTI DEVPIPQPGP GQIQVAIQAS GVCHTDLHAA EGDWPVKPNP PFIPGHEGVG FVSAVGSGVK HVKEGDRVGI PWLYTACGHC RHCLGGWETL CEEQLNTGYS VNGGFAEYVV ADPNFVGHLP KNIDFNEIAP VLCAGVTVYK GLKVTDTKPG DWVVISGIGG LGHMAVQYAR AMGLNVAAVD IDDRKLDLAR RLGATVTVNA KTVADPAAYI RKETDGGAQG VLVTAVSPKA FEQALGMVAR GGTVSLNGLP PGDFPLSIFN MVLNGVTVRG SIVGTRLDLQ ESLDFAADGK VKATIQTGKL EDINAIFDDM RQGNIEGRIV MDLTQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCAAAGACG ATGAAAGCTG CGGTCGTGCG TGCCTTCGGC AAACCGCTGA CGATTGATGA AGTGCCGATA CCTCAACCCG GTCCCGGCCA GATTCAGGTT GCCATTCAGG CTTCCGGCGT ATGCCATACC GACCTTCATG CCGCCGAGGG CGATTGGCCG GTAAAACCCA ATCCGCCCTT CATTCCGGGT CATGAGGGTG TCGGTTTCGT TTCCGCCGTG GGCAGCGGCG TCAAGCATGT CAAGGAAGGC GACCGTGTCG GCATTCCATG GCTTTACACT GCCTGCGGCC ATTGCCGCCA TTGCCTTGGT GGCTGGGAAA CGCTTTGCGA GGAACAGCTC AATACCGGCT ATTCCGTCAA TGGCGGCTTT GCCGAATATG TGGTGGCCGA TCCGAATTTC GTCGGCCACT TGCCGAAGAA TATCGATTTC AACGAGATTG CGCCGGTGCT CTGCGCGGGC GTGACGGTCT ATAAGGGCCT CAAGGTCACG GATACGAAGC CCGGTGACTG GGTGGTCATT TCAGGCATAG GCGGGCTCGG CCACATGGCT GTGCAATATG CCAGGGCCAT GGGGCTGAAT GTTGCCGCCG TCGATATTGA CGACCGCAAG CTCGATCTCG CCCGCAGACT TGGCGCAACC GTTACGGTCA ACGCTAAGAC GGTTGCCGAC CCGGCAGCCT ATATTCGCAA GGAAACCGAT GGCGGTGCGC AAGGCGTGCT GGTGACGGCG GTTTCGCCCA AGGCTTTTGA ACAGGCGCTG GGCATGGTTG CGCGCGGCGG TACTGTTTCG CTGAATGGCC TGCCGCCTGG CGATTTCCCG CTTTCCATCT TCAACATGGT GCTCAATGGC GTAACGGTGC GCGGCTCCAT CGTGGGGACG CGGCTTGATT TGCAGGAATC GCTCGATTTT GCAGCCGACG GCAAGGTCAA GGCCACCATC CAAACCGGCA AGCTGGAAGA CATCAACGCC ATCTTCGACG ATATGCGGCA AGGCAATATC GAAGGCCGCA TCGTGATGGA CCTGACTCAA TAGAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA
Details for BrabA.00044.a.B1.PW25519
| PURIFICATION DATe: | 1/19/2010 |
| CONCENTRATION: | 29.31mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 12 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA KTMKAAVVRA FGKPLTIDEV PIPQPGPGQI QVAIQASGVC HTDLHAAEGD WPVKPNPPFI PGHEGVGFVS AVGSGVKHVK EGDRVGIPWL YTACGHCRHC LGGWETLCEE QLNTGYSVNG GFAEYVVADP NFVGHLPKNI DFNEIAPVLC AGVTVYKGLK VTDTKPGDWV VISGIGGLGH MAVQYARAMG LNVAAVDIDD RKLDLARRLG ATVTVNAKTV ADPAAYIRKE TDGGAQGVLV TAVSPKAFEQ ALGMVARGGT VSLNGLPPGD FPLSIFNMVL NGVTVRGSIV GTRLDLQESL DFAADGKVKA TIQTGKLEDI NAIFDDMRQG NIEGRIVMDL TQ
Validated NT Sequence
atggctcacc accaccacca ccatatggca aagacgatga aagctgcggt cgtgcgtgcc ttcggcaaac cgctgacgat tgatgaagtg ccgatacctc aacccggtcc cggccagatt caggttgcca ttcaggcttc cggcgtatgc cataccgacc ttcatgccgc cgagggcgat tggccggtaa aacccaatcc gcccttcatt ccgggtcatg agggtgtcgg tttcgtttcc gccgtgggca gcggcgtcaa gcatgtcaag gaaggcgacc gtgtcggcat tccatggctt tacactgcct gcggccattg ccgccattgc cttggtggct gggaaacgct ttgcgaggaa cagctcaata ccggctattc cgtcaatggc ggctttgccg aatatgtggt ggccgatccg aatttcgtcg gccacttgcc gaagaatatc gatttcaacg agattgcgcc ggtgctctgc gcgggcgtga cggtctataa gggcctcaag gtcacggata cgaagcccgg tgactgggtg gtcatttcag gcataggcgg gctcggccac atggctgtgc aatatgccag ggccatgggg ctgaatgttg ccgccgtcga tattgacgac cgcaagctcg atctcgcccg cagacttggc gcaaccgtta cggtcaacgc taagacggtt gccgacccgg cagcctatat tcgcaaggaa accgatggcg gtgcgcaagg cgtgctggtg acggcggttt cgcccaaggc ttttgaacag gcgctgggca tggttgcgcg cggcggtact gtttcgctga atggcctgcc gcctggcgat ttcccgcttt ccatcttcaa catggtgctc aatggcgtaa cggtgcgcgg ctccatcgtg gggacgcggc ttgatttgca ggaatcgctc gattttgcag ccgacggcaa ggtcaaggcc accatccaaa ccggcaagct ggaagacatc aacgccatct tcgacgatat gcggcaaggc aatatcgaag gccgcatcgt gatggacctg actcaataag tgagtaagat
Expressed Protein Sequence
MAHHHHHHMA KTMKAAVVRA FGKPLTIDEV PIPQPGPGQI QVAIQASGVC HTDLHAAEGD WPVKPNPPFI PGHEGVGFVS AVGSGVKHVK EGDRVGIPWL YTACGHCRHC LGGWETLCEE QLNTGYSVNG GFAEYVVADP NFVGHLPKNI DFNEIAPVLC AGVTVYKGLK VTDTKPGDWV VISGIGGLGH MAVQYARAMG LNVAAVDIDD RKLDLARRLG ATVTVNAKTV ADPAAYIRKE TDGGAQGVLV TAVSPKAFEQ ALGMVARGGT VSLNGLPPGD FPLSIFNMVL NGVTVRGSIV GTRLDLQESL DFAADGKVKA TIQTGKLEDI NAIFDDMRQG NIEGRIVMDL TQ
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCAA AGACGATGAA AGCTGCGGTC GTGCGTGCCT TCGGCAAACC GCTGACGATT GATGAAGTGC CGATACCTCA ACCCGGTCCC GGCCAGATTC AGGTTGCCAT TCAGGCTTCC GGCGTATGCC ATACCGACCT TCATGCCGCC GAGGGCGATT GGCCGGTAAA ACCCAATCCG CCCTTCATTC CGGGTCATGA GGGTGTCGGT TTCGTTTCCG CCGTGGGCAG CGGCGTCAAG CATGTCAAGG AAGGCGACCG TGTCGGCATT CCATGGCTTT ACACTGCCTG CGGCCATTGC CGCCATTGCC TTGGTGGCTG GGAAACGCTT TGCGAGGAAC AGCTCAATAC CGGCTATTCC GTCAATGGCG GCTTTGCCGA ATATGTGGTG GCCGATCCGA ATTTCGTCGG CCACTTGCCG AAGAATATCG ATTTCAACGA GATTGCGCCG GTGCTCTGCG CGGGCGTGAC GGTCTATAAG GGCCTCAAGG TCACGGATAC GAAGCCCGGT GACTGGGTGG TCATTTCAGG CATAGGCGGG CTCGGCCACA TGGCTGTGCA ATATGCCAGG GCCATGGGGC TGAATGTTGC CGCCGTCGAT ATTGACGACC GCAAGCTCGA TCTCGCCCGC AGACTTGGCG CAACCGTTAC GGTCAACGCT AAGACGGTTG CCGACCCGGC AGCCTATATT CGCAAGGAAA CCGATGGCGG TGCGCAAGGC GTGCTGGTGA CGGCGGTTTC GCCCAAGGCT TTTGAACAGG CGCTGGGCAT GGTTGCGCGC GGCGGTACTG TTTCGCTGAA TGGCCTGCCG CCTGGCGATT TCCCGCTTTC CATCTTCAAC ATGGTGCTCA ATGGCGTAAC GGTGCGCGGC TCCATCGTGG GGACGCGGCT TGATTTGCAG GAATCGCTCG ATTTTGCAGC CGACGGCAAG GTCAAGGCCA CCATCCAAAC CGGCAAGCTG GAAGACATCA ACGCCATCTT CGACGATATG CGGCAAGGCA ATATCGAAGG CCGCATCGTG ATGGACCTGA CTCAATAGTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT