BrabA.00042.a

Elongation factor, GTP-binding:ATP/GTP-binding site motif A (P-loop):Elongation factor Tu, C-terminal:Elongation factor Tu, d...

CENTER ID: BrabA.00042.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.00042.a.A1.GE25379 Full length( BrabA.00042.a ) 1 391
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_126954
PATRIC ID: fig|359391.4.peg.1321
RefSeq: YP_414643
UniProt: Q2YM08
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAKSKFERTK PHVNIGTIGH VDHGKTSLTA AITKFFGEFK AYDQIDAAPE ERARGITIST AHVEYETANR HYAHVDCPGH ADYVKNMITG AAQMDGAILV VSAADGPMPQ TREHILLARQ VGVPAIVVFL NKCDQVDDAE LLELVELEVR ELLSKYEFPG DEIPIIKGSA LAALEDSSKE LGEDAIRNLM DAVDSYIPTP ERPIDQPFLM PIEDVFSISG RGTVVTGRVE RGIVKVGEEV EIVGIKATTK TTVTGVEMFR KLLDQGQAGD NIGALIRGVG REDVERGQVL CKPGSVKPHT KFKAEAYILT KDEGGRHTPF FTNYRPQFYF RTTDVTGVVT LPAGTEMVMP GDNVAMDVTL IVPIAMEEKL RFAIREGGRT VGAGIVSSII E
NT Sequence
ATGGCAAAGA GTAAGTTTGA ACGTACGAAG CCCCACGTTA ACATCGGCAC GATTGGTCAC GTTGACCATG GCAAGACGTC GCTGACGGCG GCGATCACGA AGTTTTTCGG CGAATTCAAG GCGTATGACC AGATCGACGC CGCGCCTGAA GAACGCGCCC GCGGCATCAC GATCTCGACG GCCCACGTCG AGTACGAGAC CGCAAACCGT CACTATGCAC ACGTCGATTG CCCCGGCCAC GCCGACTATG TGAAGAACAT GATCACCGGC GCTGCCCAGA TGGATGGCGC GATCCTGGTG GTTTCGGCTG CTGACGGCCC GATGCCGCAG ACCCGCGAGC ACATCCTGCT TGCCCGTCAG GTTGGCGTTC CGGCGATCGT CGTGTTCCTC AACAAGTGCG ACCAGGTTGA CGATGCAGAA CTGCTCGAAC TGGTTGAACT GGAAGTGCGC GAACTTCTGT CGAAGTACGA ATTCCCCGGC GACGAAATCC CGATCATCAA GGGCTCGGCT CTTGCTGCTC TGGAAGATTC TTCCAAGGAA CTGGGCGAAG ATGCCATCCG CAACCTGATG GACGCGGTTG ACAGCTACAT TCCGACCCCG GAACGCCCGA TCGACCAGCC GTTCCTGATG CCGATCGAAG ACGTGTTCTC GATCTCCGGC CGTGGTACGG TTGTGACGGG TCGCGTTGAG CGCGGTATCG TTAAGGTCGG TGAAGAAGTT GAAATCGTCG GCATCAAGGC GACGACGAAG ACCACGGTTA CCGGCGTTGA AATGTTCCGC AAGCTGCTCG ACCAGGGCCA GGCTGGCGAC AACATTGGCG CGCTGATCCG CGGCGTTGGC CGTGAAGACG TTGAACGCGG CCAGGTTCTC TGCAAGCCGG GTTCTGTGAA GCCGCACACC AAGTTTAAGG CAGAAGCCTA TATTCTGACC AAGGACGAAG GTGGCCGTCA TACGCCGTTC TTCACCAACT ACCGTCCGCA GTTCTACTTC CGTACGACGG ACGTGACGGG TGTTGTGACG CTTCCGGCTG GCACGGAAAT GGTCATGCCT GGCGATAACG TCGCCATGGA CGTTACCCTG ATCGTGCCGA TCGCCATGGA AGAGAAGCTT CGCTTCGCTA TCCGTGAAGG CGGCCGCACC GTCGGTGCCG GCATCGTTTC GTCGATCATC GAG
Details for BrabA.00042.a.A1.GE25379
HARVESTED ON: 6/30/2008
SEQUENCED ON: 6/16/2009
EXPECTED MW: 44kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 73
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAKSKFERT KPHVNIGTIG HVDHGKTSLT AAITKFFGEF KAYDQIDAAP EERARGITIS TAHVEYETAN RHYAHVDCPG HADYVKNMIT GAAQMDGAIL VVSAADGPMP QTREHILLAR QVGVPAIVVF LNKCDQVDDA ELLELVELEV RELLSKYEFP GDEIPIIKGS ALAALEDSSK ELGEDAIRNL MDAVDSYIPT PERPIDQPFL MPIEDVFSIS GRGTVVTGRV ERGIVXVGEE XEIVGIKATT XTTVTGVEMX RKLLDQGXXG DNIGALIRGV GR
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcaa agagtaagtt tgaacgtacg aagccccacg ttaacatcgg cacgattggt cacgttgacc atggcaagac gtcgctgacg gcggcgatca cgaagttttt cggcgaattc aaggcgtatg accagatcga cgccgcgcct gaagaacgcg cccgcggcat cacgatctcg acggcccacg tcgagtacga gaccgcaaac cgtcactatg cacacgtcga ttgccccggc cacgccgact atgtgaagaa catgatcacc ggcgctgccc agatggatgg cgcgatcctg gtggtttcgg ctgctgacgg cccgatgccg cagacccgcg agcacatcct gcttgcccgt caggttggcg ttccggcgat cgtcgtgttc ctcaacaagt gcgaccaggt tgacgatgca gaactgctcg aactggttga actggaagtg cgcgaacttc tgtcgaagta cgaattcccc ggcgacgaaa tcccgatcat caagggctcg gctcttgctg ctctggaaga ttcttccaag gaactgggcg aagatgccat ccgcaacctg atggacgcgg ttgacagcta cattccgacc ccggaacgcc cgatcgacca gccgttcctg atgccgatcg aagacgtgtt ctcgatctcc ggccgtggta cggttgtgac gggtcgcgtt gagcgcggta tcgttaangt cggtgaagaa nttgaaatcg tcggcatcaa ggcgacgacg aanaccacgg ttaccggcgt tgaaatgntc cgcaagctgc tcgaccaggg ccnggntggc gacaacattg gcgcgctgat ccgcggcgtt ggccgtgna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAKSKFERT KPHVNIGTIG HVDHGKTSLT AAITKFFGEF KAYDQIDAAP EERARGITIS TAHVEYETAN RHYAHVDCPG HADYVKNMIT GAAQMDGAIL VVSAADGPMP QTREHILLAR QVGVPAIVVF LNKCDQVDDA ELLELVELEV RELLSKYEFP GDEIPIIKGS ALAALEDSSK ELGEDAIRNL MDAVDSYIPT PERPIDQPFL MPIEDVFSIS GRGTVVTGRV ERGIVKVGEE VEIVGIKATT KTTVTGVEMF RKLLDQGQAG DNIGALIRGV GREDVERGQV LCKPGSVKPH TKFKAEAYIL TKDEGGRHTP FFTNYRPQFY FRTTDVTGVV TLPAGTEMVM PGDNVAMDVT LIVPIAMEEK LRFAIREGGR TVGAGIVSSI IE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcaaagagt aagtttgaac gtacgaagcc ccacgttaac atcggcacga ttggtcacgt tgaccatggc aagacgtcgc tgacggcggc gatcacgaag tttttcggcg aattcaaggc gtatgaccag atcgacgccg cgcctgaaga acgcgcccgc ggcatcacga tctcgacggc ccacgtcgag tacgagaccg caaaccgtca ctatgcacac gtcgattgcc ccggccacgc cgactatgtg aagaacatga tcaccggcgc tgcccagatg gatggcgcga tcctggtggt ttcggctgct gacggcccga tgccgcagac ccgcgagcac atcctgcttg cccgtcaggt tggcgttccg gcgatcgtcg tgttcctcaa caagtgcgac caggttgacg atgcagaact gctcgaactg gttgaactgg aagtgcgcga acttctgtcg aagtacgaat tccccggcga cgaaatcccg atcatcaagg gctcggctct tgctgctctg gaagattctt ccaaggaact gggcgaagat gccatccgca acctgatgga cgcggttgac agctacattc cgaccccgga acgcccgatc gaccagccgt tcctgatgcc gatcgaagac gtgttctcga tctccggccg tggtacggtt gtgacgggtc gcgttgagcg cggtatcgtt aaggtcggtg aagaagttga aatcgtcggc atcaaggcga cgacgaagac cacggttacc ggcgttgaaa tgttccgcaa gctgctcgac cagggccagg ctggcgacaa cattggcgcg ctgatccgcg gcgttggccg tgaagacgtt gaacgcggcc aggttctctg caagccgggt tctgtgaagc cgcacaccaa gtttaaggca gaagcctata ttctgaccaa ggacgaaggt ggccgtcata cgccgttctt caccaactac cgtccgcagt tctacttccg tacgacggac gtgacgggtg ttgtgacgct tccggctggc acggaaatgg tcatgcctgg cgataacgtc gccatggacg ttaccctgat cgtgccgatc gccatggaag agaagcttcg cttcgctatc cgtgaaggcg gccgcaccgt cggtgccggc atcgtttcgt cgatcatcga gtaaaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa