SSGCID: Target Page

BrabA.00039.a

Metallopeptidase family M24:Methionine aminopeptidase:Methionine aminopeptidase, subfamily 1

CENTER ID:	BrabA.00039.a
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.00039.a.A1.GE25220	Full length( BrabA.00039.a )		1	275

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_126721
PATRIC ID:	fig\|359391.4.peg.1353
RefSeq:	YP_414683
UniProt:	Q2YRG0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVTYIEATSA PVKYTGQIRL YGPEAFEAMR KVCNLTARCL DALNDIVKPG VTTNEIDRFV FEFGMDHDAF PATLNYRGYT KSTCTSINHV VCHGIPNDKP LREGDIVNID VTYLLDGWHG DSSRMYAVGE IKRASERLLE VTYESLLRGI AAVKPGAKTG AIGAAIQTYA ESERCSVVRD FCGHGVGRLF HDAPNILHYG TPNEGVEIKE GMIFTIEPMI NLGKPHVKVL ADGWTAVTRD RSLTAQYEHT VGVTRDGCEI FTLSPANIFG PPSMR

NT Sequence

ATGGTAACCT ATATCGAAGC GACAAGCGCA CCCGTGAAAT ATACAGGCCA GATCAGGCTC TACGGCCCCG AAGCCTTCGA GGCGATGCGC AAGGTCTGCA ACCTGACGGC ACGTTGCCTT GATGCGCTGA ACGATATCGT CAAACCCGGC GTGACCACCA ATGAGATCGA CCGTTTCGTA TTTGAATTCG GCATGGACCA CGATGCGTTT CCGGCAACGC TGAATTATCG CGGCTACACG AAATCGACCT GCACATCGAT CAACCATGTG GTCTGCCACG GCATCCCGAA CGACAAGCCT CTGCGCGAAG GCGATATCGT CAATATCGAC GTGACTTATC TGCTTGACGG CTGGCACGGA GATTCCAGCC GCATGTATGC GGTTGGTGAA ATCAAGCGCG CTTCCGAACG TCTGCTGGAA GTGACCTATG AAAGCCTTCT TCGCGGTATC GCCGCCGTCA AGCCGGGTGC GAAGACCGGC GCCATCGGCG CGGCGATCCA GACCTATGCC GAAAGCGAAC GCTGCTCGGT GGTGCGCGAT TTCTGCGGTC ATGGCGTCGG CCGGCTTTTC CATGATGCGC CGAATATCCT GCACTACGGC ACCCCCAATG AAGGTGTCGA GATCAAGGAA GGCATGATCT TCACGATCGA GCCGATGATC AATCTGGGCA AACCGCATGT GAAGGTTCTT GCCGATGGCT GGACGGCGGT GACGCGGGAC CGCTCCTTGA CCGCACAATA TGAACACACG GTCGGCGTCA CCAGGGATGG CTGCGAGATT TTCACGCTTT CTCCGGCCAA TATCTTTGGC CCGCCTTCCA TGCGG

Details for BrabA.00039.a.A1.GE25220

HARVESTED ON:	5/6/2008
SEQUENCED ON:	6/16/2009
EXPECTED MW:	32kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVTYIEATS APVKYTGQIR LYGPEAFEAM RKVCNLTARC LDALNDIVKP GVTTNEIDRF VFEFGMDHDA FPATLNYRGY TKSTCTSINH VVCHGIPNDK PLREGDIVNI DVTYLLDGWH GDSSRMYAVG EIKRASERLL EVTYESLLRG IAAVKPGAKT GAIGAAIQTY AESERCSVVR DFCGHGVGRL FHDAPNILHY GTPNEGVEIK EGMIFTIEPM INLGKPHVKV LADGWTAVTR DRSLTAQYEH TVGVTRDGCE IFTLSPANIF GPPSMR

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtaa cctatatcga agcgacaagc gcacccgtga aatatacagg ccagatcagg ctctacggcc ccgaagcctt cgaggcgatg cgcaaggtct gcaacctgac ggcacgttgc cttgatgcgc tgaacgatat cgtcaaaccc ggcgtgacca ccaatgagat cgaccgtttc gtatttgaat tcggcatgga ccacgatgcg tttccggcaa cgctgaatta tcgcggctac acgaaatcga cctgcacatc gatcaaccat gtggtctgcc acggcatccc gaacgacaag cctctgcgcg aaggcgatat cgtcaatatc gacgtgactt atctgcttga cggctggcac ggagattcca gccgcatgta tgcggttggt gaaatcaagc gcgcttccga acgtctgctg gaagtgacct atgaaagcct tcttcgcggt atcgccgccg tcaagccggg tgcgaagacc ggcgccatcg gcgcggcgat ccagacctat gccgaaagcg aacgctgctc ggtggtgcgc gatttctgcg gtcatggcgt cggccggctt ttccatgatg cgccgaatat cctgcactac ggcaccccca atgaaggtgt cgagatcaag gaaggcatga tcttcacgat cgagccgatg atcaatctgg gcaaaccgca tgtgaaggtt cttgccgatg gctggacggc ggtgacgcgg gaccgctcct tgaccgcaca atatgaacac acggtcggcg tcaccaggga tggctgcgag attttcacgc tttctccggc caatatcttt ggcccgcctt ccatgcggta ataa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtaacctat atcgaagcga caagcgcacc cgtgaaatat acaggccaga tcaggctcta cggccccgaa gccttcgagg cgatgcgcaa ggtctgcaac ctgacggcac gttgccttga tgcgctgaac gatatcgtca aacccggcgt gaccaccaat gagatcgacc gtttcgtatt tgaattcggc atggaccacg atgcgtttcc ggcaacgctg aattatcgcg gctacacgaa atcgacctgc acatcgatca accatgtggt ctgccacggc atcccgaacg acaagcctct gcgcgaaggc gatatcgtca atatcgacgt gacttatctg cttgacggct ggcacggaga ttccagccgc atgtatgcgg ttggtgaaat caagcgcgct tccgaacgtc tgctggaagt gacctatgaa agccttcttc gcggtatcgc cgccgtcaag ccgggtgcga agaccggcgc catcggcgcg gcgatccaga cctatgccga aagcgaacgc tgctcggtgg tgcgcgattt ctgcggtcat ggcgtcggcc ggcttttcca tgatgcgccg aatatcctgc actacggcac ccccaatgaa ggtgtcgaga tcaaggaagg catgatcttc acgatcgagc cgatgatcaa tctgggcaaa ccgcatgtga aggttcttgc cgatggctgg acggcggtga cgcgggaccg ctccttgacc gcacaatatg aacacacggt cggcgtcacc agggatggct gcgagatttt cacgctttct ccggccaata tctttggccc gccttccatg cggtgaaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa