BrabA.00036.a

Acetyl-CoA carboxylase, biotin carboxylase:Carbamoyl-phosphate synthase L chain, ATP-binding:Carbamoyl-phosphate synthetase l...

CENTER ID: BrabA.00036.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.00036.a.B1.PW25515 Full length( BrabA.00036.a ) 1 452
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_126626
PATRIC ID: fig|359391.4.peg.961
RefSeq: YP_414349
UniProt: Q2YNP3
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFQKILIANR GEIALRVLRA CKELGIKTVA VHSTADADAM HVRLADESVC IGPPPSRDSY LNIHQIVAAC EITGADAIHP GYGFLSENAK FAEILEAHDI TFIGPTASHI RIMGDKIEAK RTAKRLGIPV VPGSDGGVTD EVEAARIAKE IGYPVIIKAS AGGGGRGMKV ARSAEELPIA LATARSEAGA AFGDDAVYIE KYLEKPRHIE VQVMGDGAGN AVHLGERDCS LQRRHQKVWE EGNSPALNAE AREKIGMICA NACAELGYRG AGTIEFLYED GEFYFIEMNT RLQVEHPVTE AITGIDLVHE QIRVAAGLGL SVKQKDIRFS GHAIECRINA EDPLTFAPSP GLITHYHTPG GLGVRVDSGV YSGYRIPPYY DSLIGKLIVH GRNRVECMMR LRRALDEFVV DGVKTTLPLF QDLISNQDIA NGDYDIHWLE KYLANKSKNE SA
NT Sequence
ATGTTTCAAA AGATACTCAT AGCCAATCGT GGCGAAATCG CTCTTCGCGT GCTCAGGGCT TGCAAGGAGC TGGGCATAAA GACGGTCGCC GTTCATTCCA CGGCAGATGC CGACGCAATG CATGTGCGCC TGGCAGATGA GAGCGTATGC ATCGGACCGC CGCCCTCACG CGACAGCTAT CTGAACATCC ATCAGATCGT GGCCGCCTGC GAAATCACCG GCGCGGATGC AATTCACCCC GGCTACGGCT TCCTTTCGGA AAATGCAAAA TTCGCCGAAA TCCTTGAAGC CCATGACATT ACCTTCATCG GCCCCACTGC TTCGCATATC CGCATCATGG GCGACAAGAT CGAAGCCAAG CGCACCGCAA AGCGCCTCGG CATCCCGGTC GTTCCGGGTT CGGACGGCGG CGTGACGGAT GAAGTCGAAG CAGCCCGTAT CGCCAAGGAA ATCGGCTATC CGGTCATCAT CAAGGCTTCG GCCGGCGGCG GTGGTCGTGG CATGAAGGTC GCCCGCAGCG CGGAAGAGCT TCCGATCGCA CTTGCGACGG CACGTTCTGA AGCTGGCGCG GCCTTTGGCG ACGATGCGGT CTACATCGAA AAATATCTCG AAAAACCGCG CCATATCGAA GTTCAGGTCA TGGGGGACGG TGCGGGCAAT GCCGTCCATC TGGGCGAACG CGACTGCTCG CTGCAACGCC GTCACCAGAA GGTCTGGGAA GAGGGCAACT CCCCCGCCCT CAATGCGGAA GCGCGCGAAA AGATCGGCAT GATCTGCGCC AATGCCTGCG CAGAGCTTGG CTATCGCGGC GCAGGCACGA TCGAATTTCT CTATGAAGAT GGAGAATTCT ATTTCATCGA AATGAATACG CGCCTTCAGG TGGAACATCC GGTTACGGAA GCGATCACCG GCATCGATCT TGTGCATGAG CAGATTCGCG TTGCCGCAGG CCTTGGTCTT TCGGTGAAGC AGAAGGATAT CCGCTTTTCC GGCCATGCCA TCGAGTGCCG TATCAATGCG GAAGATCCGC TGACCTTCGC ACCGTCACCG GGCCTCATCA CGCATTATCA TACGCCGGGC GGGCTTGGCG TACGCGTCGA TTCCGGCGTT TATTCCGGCT ATCGCATTCC ACCCTACTAT GACAGCCTGA TCGGCAAGCT CATCGTGCAT GGACGTAACC GCGTCGAATG CATGATGCGT CTGCGCCGCG CGCTGGATGA GTTCGTGGTG GATGGGGTAA AGACCACCCT GCCCCTGTTT CAGGATCTGA TTTCCAATCA GGATATCGCC AATGGCGATT ATGACATCCA CTGGTTGGAA AAGTACCTGG CCAACAAGAG CAAGAACGAA AGCGCC
Details for BrabA.00036.a.B1.PW25515
PURIFICATION DATe: 1/19/2010
CONCENTRATION: 24.03mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 16
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMF QKILIANRGE IALRVLRACK ELGIKTVAVH STADADAMHV RLADESVCIG PPPSRDSYLN IHQIVAACEI TGADAIHPGY GFLSENAKFA EILEAHDITF IGPTASHIRI MGDKIEAKRT AKRLGIPVVP GSDGGVTDEV EAARIAKEIG YPVIIKASAG GGGRGMKVAR SAEELPIALA TARSEAGAAF GDDAVYIEKY LEKPRHIEVQ VMGDGAGNAV HLGERDCSLQ RRHQKVWEEG NSPALNAEAR EKIGMICANA CAELGYRGAG TIEFLYEDGE FYFIEMNTRL QVEHPVTEAI TGIDLVHEQI RVAAGLGLSV KQKDIRFSGH AIECRINAED PLTFAPSPGL ITHYHTPGGL GVRVDSGVYS GYRIPPYYDS LIGKLIVHGR NRVECMMRLR RALDEFVVDG VKTTLPLFQD LISXXDIANG DYDIHWLEKY LAN
Validated NT Sequence
ttaagaagga ganataccat ggctcaccac caccaccacc atatgtttca aaagatactc atagccaatc gtggcgaaat cgctctacgc gtgctcaggg cttgcaagga gctgggcata aagacggtcg ccgttcattc cacggcagat gccgacgcaa tgcatgtgcg cctggcagat gagagcgtat gcatcggacc gccgccctca cgcgacagct atctgaacat ccatcagatc gtggccgcct gcgaaatcac cggcgcggat gcaattcacc ccggctacgg cttcctttcg gaaaatgcaa aattcgccga aatccttgaa gcccatgaca ttaccttcat cggccccact gcttcgcata tccgcatcat gggcgacaag atcgaagcca agcgcaccgc aaagcgcctc ggcatcccgg tcgttccggg ttcggacggc ggcgtgacgg atgaagtcga agcagcccgt atcgccaagg aaatcggcta tccggtcatc atcaaggctt cggccggcgg cggtggtcgt ggcatgaagg tcgcccgcag cgcggaagag cttccgatcg cacttgcgac ggcacgttct gaagctggcg cggcctttgg tgacgatgcg gtctacatcg aaaaatatct cgaaaaaccg cgccatatcg aagttcaggt catgggggac ggtgcgggca atgccgtcca tctgggcgaa cgcgactgct cgctgcaacg ccgtcaccag aaggtctggg aagagggcaa ctcccccgcc ctcaatgcgg aagcgcgcga aaagatcggc atgatctgcg ccaatgcctg cgcagagctt ggctatcgcg gcgcaggcac gatcgaattt ctctatgaag atggagaatt ctatttcatc gaaatgaata cgcgccttca ggtggaacat ccggttacgg aagcgatcac cggcatcgat cttgtgcatg agcagattcg cgttgccgca ggccttggtc tttcggtgaa gcagaaggat atccgctttt ccggccatgc catcgagtgc cgtatcaatg cggaagatcc gctgaccttc gcaccgtcac cgggcctcat cacgcattat catacgccgg gcgggcttgg cgtacgcgtc gattccggcg tttattccgg ctatcgcatt ccaccctact atgacagcct gatcggcaag ctcatcgtgc atggacgtaa ccgcgtcgaa tgcatgatgc gtctgcgccg cgcgctggat gagttcgtgg tggatggggt aaagaccacc ctgcccctgt ttcaggatct gatttccann cnggatatcg ccaatggcga ttatgacatc cactggttgg aaaagtacct ggccaacaag
Expressed Protein Sequence
MAHHHHHHMF QKILIANRGE IALRVLRACK ELGIKTVAVH STADADAMHV RLADESVCIG PPPSRDSYLN IHQIVAACEI TGADAIHPGY GFLSENAKFA EILEAHDITF IGPTASHIRI MGDKIEAKRT AKRLGIPVVP GSDGGVTDEV EAARIAKEIG YPVIIKASAG GGGRGMKVAR SAEELPIALA TARSEAGAAF GDDAVYIEKY LEKPRHIEVQ VMGDGAGNAV HLGERDCSLQ RRHQKVWEEG NSPALNAEAR EKIGMICANA CAELGYRGAG TIEFLYEDGE FYFIEMNTRL QVEHPVTEAI TGIDLVHEQI RVAAGLGLSV KQKDIRFSGH AIECRINAED PLTFAPSPGL ITHYHTPGGL GVRVDSGVYS GYRIPPYYDS LIGKLIVHGR NRVECMMRLR RALDEFVVDG VKTTLPLFQD LISNQDIANG DYDIHWLEKY LANKSKNESA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTTC AAAAGATACT CATAGCCAAT CGTGGCGAAA TCGCTCTTCG CGTGCTCAGG GCTTGCAAGG AGCTGGGCAT AAAGACGGTC GCCGTTCATT CCACGGCAGA TGCCGACGCA ATGCATGTGC GCCTGGCAGA TGAGAGCGTA TGCATCGGAC CGCCGCCCTC ACGCGACAGC TATCTGAACA TCCATCAGAT CGTGGCCGCC TGCGAAATCA CCGGCGCGGA TGCAATTCAC CCCGGCTACG GCTTCCTTTC GGAAAATGCA AAATTCGCCG AAATCCTTGA AGCCCATGAC ATTACCTTCA TCGGCCCCAC TGCTTCGCAT ATCCGCATCA TGGGCGACAA GATCGAAGCC AAGCGCACCG CAAAGCGCCT CGGCATCCCG GTCGTTCCGG GTTCGGACGG CGGCGTGACG GATGAAGTCG AAGCAGCCCG TATCGCCAAG GAAATCGGCT ATCCGGTCAT CATCAAGGCT TCGGCCGGCG GCGGTGGTCG TGGCATGAAG GTCGCCCGCA GCGCGGAAGA GCTTCCGATC GCACTTGCGA CGGCACGTTC TGAAGCTGGC GCGGCCTTTG GCGACGATGC GGTCTACATC GAAAAATATC TCGAAAAACC GCGCCATATC GAAGTTCAGG TCATGGGGGA CGGTGCGGGC AATGCCGTCC ATCTGGGCGA ACGCGACTGC TCGCTGCAAC GCCGTCACCA GAAGGTCTGG GAAGAGGGCA ACTCCCCCGC CCTCAATGCG GAAGCGCGCG AAAAGATCGG CATGATCTGC GCCAATGCCT GCGCAGAGCT TGGCTATCGC GGCGCAGGCA CGATCGAATT TCTCTATGAA GATGGAGAAT TCTATTTCAT CGAAATGAAT ACGCGCCTTC AGGTGGAACA TCCGGTTACG GAAGCGATCA CCGGCATCGA TCTTGTGCAT GAGCAGATTC GCGTTGCCGC AGGCCTTGGT CTTTCGGTGA AGCAGAAGGA TATCCGCTTT TCCGGCCATG CCATCGAGTG CCGTATCAAT GCGGAAGATC CGCTGACCTT CGCACCGTCA CCGGGCCTCA TCACGCATTA TCATACGCCG GGCGGGCTTG GCGTACGCGT CGATTCCGGC GTTTATTCCG GCTATCGCAT TCCACCCTAC TATGACAGCC TGATCGGCAA GCTCATCGTG CATGGACGTA ACCGCGTCGA ATGCATGATG CGTCTGCGCC GCGCGCTGGA TGAGTTCGTG GTGGATGGGG TAAAGACCAC CCTGCCCCTG TTTCAGGATC TGATTTCCAA TCAGGATATC GCCAATGGCG ATTATGACAT CCACTGGTTG GAAAAGTACC TGGCCAACAA GAGCAAGAAC GAAAGCGCCT GATGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT