BrabA.00032.a
S-adenosyl-L-homocysteine hydrolase:ATP/GTP-binding site motif A (P-loop)
| CENTER ID: | BrabA.00032.a |
| ORGANISM: | Brucella melitensis biovar Abortus 2308 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.00032.a.A2.GE28020 | Created from Decode construct for feature BrabA.00032.a | 1 | 453 | ||
| BrabA.00032.a.A3.GE28021 | Created from Decode construct for feature BrabA.00032.a | 4 | 466 | ||
| BrabA.00032.a.A4.GE28022 | Created from Decode construct for feature BrabA.00032.a | 4 | 453 | ||
| BrabA.00032.a.A5.GE28023 | Created from Decode construct for feature BrabA.00032.a | 8 | 466 | ||
| BrabA.00032.a.A6.GE28024 | Created from Decode construct for feature BrabA.00032.a | 8 | 453 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party
can then use to order the clone from a third-party clone manufacturer.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.00032.a.A2.PS00516 | Created from Decode construct for feature BrabA.00032.a | 1 | 453 | ||
| BrabA.00032.a.A3.PS00530 | Created from Decode construct for feature BrabA.00032.a | 4 | 466 | ||
| BrabA.00032.a.A4.PS00520 | Created from Decode construct for feature BrabA.00032.a | 4 | 453 | ||
| BrabA.00032.a.A5.PS00514 | Created from Decode construct for feature BrabA.00032.a | 8 | 466 | ||
| BrabA.00032.a.A6.PS00517 | Created from Decode construct for feature BrabA.00032.a | 8 | 453 | ||
| BrabA.00032.a.B1.PW29481 | Full length( BrabA.00032.a ) | 1 | 466 | ||
| BrabA.00032.a.B1.PW25512 | Full length( BrabA.00032.a ) | 1 | 466 |
Structures
3N58
DEPOSITED: 5/24/2010
DETERMINATION: XRay
CLONE: BrabA.00032.a.A5.GE28023
PROTEIN: BrabA.00032.a.A5.PS00514
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|359391.4.peg.2185 |
| RefSeq: | YP_415424 |
| UniProt: | Q2YQX8 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTASQDFVVK DISLADWGRK ELDIAETEMP GLMAAREEFG KSQPLKGARI SGSLHMTIQT AVLIETLKVL GAEVRWASCN IFSTQDHAAA AIAATGTPVF AVKGETLEEY WTYTDQIFQW PDGEPSNMIL DDGGDATMYI LIGARAEAGE DVLSNPQSEE EEVLFAQIKK RMAATPGFFT KQRAAIKGVT EETTTGVNRL YQLQKKGLLP FPAINVNDSV TKSKFDNKYG CKESLVDGIR RGTDVMMAGK VAVVCGYGDV GKGSAQSLAG AGARVKVTEV DPICALQAAM DGFEVVTLDD AASTADIVVT TTGNKDVITI DHMRKMKDMC IVGNIGHFDN EIQVAALRNL KWTNVKPQVD LIEFPDGKRL ILLSEGRLLN LGNATGHPSF VMSASFTNQV LGQIELFTRT DAYKNEVYVL PKHLDEKVAR LHLDKLGAKL TVLSEEQAAY IGVTPQGPFK SEHYRY
NT Sequence
ATGACCGCAA GCCAAGATTT TGTTGTCAAG GATATTAGCC TGGCCGATTG GGGCCGCAAG GAACTCGACA TCGCAGAAAC CGAAATGCCG GGCCTGATGG CTGCCCGCGA GGAATTCGGC AAGTCGCAGC CGCTGAAGGG CGCGCGCATT TCCGGCTCGC TGCACATGAC CATTCAAACT GCCGTTCTCA TTGAGACGCT GAAGGTTCTG GGTGCCGAAG TGCGCTGGGC TTCCTGCAAC ATTTTCTCCA CGCAGGATCA CGCGGCGGCG GCGATTGCGG CAACCGGCAC CCCGGTTTTC GCCGTCAAGG GCGAGACGCT TGAAGAATAC TGGACCTATA CCGACCAGAT CTTCCAGTGG CCGGATGGCG AGCCGTCCAA CATGATCCTC GATGATGGCG GCGACGCCAC CATGTATATT CTCATCGGCG CGCGCGCCGA GGCTGGCGAG GACGTCCTGT CCAATCCGCA GAGCGAGGAA GAAGAAGTTC TTTTCGCGCA GATCAAGAAG CGCATGGCCG CAACGCCGGG CTTCTTCACG AAGCAGCGCG CCGCAATCAA GGGCGTGACG GAAGAAACGA CCACCGGCGT CAACCGCCTC TATCAGTTGC AGAAGAAGGG TCTCCTGCCC TTCCCCGCCA TCAACGTCAA TGACAGTGTG ACCAAGTCGA AGTTCGACAA CAAGTATGGC TGCAAGGAAT CGCTGGTGGA TGGCATTCGT CGCGGCACCG ACGTCATGAT GGCCGGCAAG GTTGCCGTCG TCTGCGGCTA TGGTGATGTC GGCAAAGGCT CTGCCCAGTC GCTCGCCGGT GCCGGCGCTC GCGTCAAGGT CACGGAAGTC GATCCGATCT GCGCGCTTCA GGCCGCGATG GACGGCTTCG AGGTCGTGAC CCTTGATGAT GCCGCCTCCA CCGCCGATAT CGTCGTGACG ACCACCGGCA ACAAGGACGT CATCACGATC GACCATATGC GCAAGATGAA AGACATGTGC ATCGTCGGCA ATATCGGCCA CTTCGACAAT GAAATTCAGG TCGCAGCCCT GCGCAACCTG AAATGGACCA ATGTGAAGCC GCAGGTCGAT CTGATCGAGT TCCCCGACGG CAAGCGCCTC ATCCTTCTTT CGGAAGGCCG CCTGCTCAAT CTGGGCAATG CGACGGGCCA TCCAAGCTTT GTCATGTCGG CTTCCTTCAC CAATCAGGTG TTGGGCCAGA TCGAACTTTT CACCCGCACC GATGCCTATA AGAACGAAGT CTATGTGCTG CCGAAGCACC TTGATGAAAA GGTCGCTCGT CTTCATCTCG ACAAGCTTGG CGCAAAGCTG ACGGTACTTT CCGAGGAACA GGCTGCCTAT ATCGGCGTCA CTCCACAGGG TCCGTTCAAG TCGGAACACT ACAGGTAT
Details for BrabA.00032.a.A2.GE28020
| HARVESTED ON: | 12/7/2009 |
| SEQUENCED ON: | 12/23/2009 |
| EXPECTED MW: | 51kDa |
| OBSERVED MW: | 51kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 96 |
| PERCENT COVERAGE: | 59 |
Validated AA Sequence
MTASQDFVVK DISLADWGRK ELDIAETEMP GLMAAREEFG KSQPLKGARI SGSLHMTIQT AVLIETLKVL GAEVRWASCN IFSTQDHAAA AIAATGTPVF AVKGETLEEY WTYTDQIFQW PDGEPSNMIL DDGGDATMYI LIGARAEAGE DVLSNPQSEE EEVLFAQIKK RMAATPGFFT KQRAAIKGVX EETTTGVNRL YQLQKKGLLP FPAINVNDSV XKSKFDXXYG CXESLXDGXR RGTDVMMAGK VAVVCGYGDV GKXSAQSLAG AGARVKVTEX X
Validated NT Sequence
aagctcagac ccagggtcct ggttcgatga ccgcaagcca agattttgtt gtcaaggata ttagcctggc cgattggggc cgcaaggaac tcgacatcgc agaaaccgaa atgccgggcc tgatggctgc ccgcgaggaa ttcggcaagt cgcagccgct gaagggcgcg cgcatttccg gctcgctgca catgaccatt caaactgccg ttctcattga gacgctgaag gttctgggtg ccgaagtgcg ctgggcttcc tgcaacattt tctccacgca ggatcacgcg gcggcggcga ttgcggcaac cggcaccccg gttttcgccg tcaagggcga gacgcttgaa gaatactgga cctataccga ccagatcttc cagtggccgg atggcgagcc gtccaacatg atcctcgatg atggcggcga cgccaccatg tatattctca tcggcgcgcg cgccgaggct ggcgaggacg tcctgtccaa tccgcagagc gaggaagaag aagttctttt cgcgcagatc aagaagcgca tggccgcaac gccgggcttc ttcacgaagc agcgcgccgc aatcaagggc gtgncggaag aaacgaccac cggcgtcaac cgcctctatc agttgcagaa gaagggtctc ctgcccttcc ccgccatcaa cgtcaatgac agtgtganca agtcgaagtt cgacnacnag tatggctgcn aggaatcgct ggnggatggc nttcgtcgcg gcaccgacgt catgatggcc ggnaaggttg ccgtcgtctg cggctatggt gatgtcggca aangntctgc ccagtcgctc gccggngccg gcgctcgcgt caaggtcacg gaantcnatc c
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTASQDFVV KDISLADWGR KELDIAETEM PGLMAAREEF GKSQPLKGAR ISGSLHMTIQ TAVLIETLKV LGAEVRWASC NIFSTQDHAA AAIAATGTPV FAVKGETLEE YWTYTDQIFQ WPDGEPSNMI LDDGGDATMY ILIGARAEAG EDVLSNPQSE EEEVLFAQIK KRMAATPGFF TKQRAAIKGV TEETTTGVNR LYQLQKKGLL PFPAINVNDS VTKSKFDNKY GCKESLVDGI RRGTDVMMAG KVAVVCGYGD VGKGSAQSLA GAGARVKVTE VDPICALQAA MDGFEVVTLD DAASTADIVV TTTGNKDVIT IDHMRKMKDM CIVGNIGHFD NEIQVAALRN LKWTNVKPQV DLIEFPDGKR LILLSEGRLL NLGNATGHPS FVMSASFTNQ VLGQIELFTR TDAYKNEVYV LPKHLDEKVA RLHLDKLGAK LTVLSEEQAA YIGV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgcaagc caagattttg ttgtcaagga tattagcctg gccgattggg gccgcaagga actcgacatc gcagaaaccg aaatgccggg cctgatggct gcccgcgagg aattcggcaa gtcgcagccg ctgaagggcg cgcgcatttc cggctcgctg cacatgacca ttcaaactgc cgttctcatt gagacgctga aggttctggg tgccgaagtg cgctgggctt cctgcaacat tttctccacg caggatcacg cggcggcggc gattgcggca accggcaccc cggttttcgc cgtcaagggc gagacgcttg aagaatactg gacctatacc gaccagatct tccagtggcc ggatggcgag ccgtccaaca tgatcctcga tgatggcggc gacgccacca tgtatattct catcggcgcg cgcgccgagg ctggcgagga cgtcctgtcc aatccgcaga gcgaggaaga agaagttctt ttcgcgcaga tcaagaagcg catggccgca acgccgggct tcttcacgaa gcagcgcgcc gcaatcaagg gcgtgacgga agaaacgacc accggcgtca accgcctcta tcagttgcag aagaagggtc tcctgccctt ccccgccatc aacgtcaatg acagtgtgac caagtcgaag ttcgacaaca agtatggctg caaggaatcg ctggtggatg gcattcgtcg cggcaccgac gtcatgatgg ccggcaaggt tgccgtcgtc tgcggctatg gtgatgtcgg caaaggctct gcccagtcgc tcgccggtgc cggcgctcgc gtcaaggtca cggaagtcga tccgatctgc gcgcttcagg ccgcgatgga cggcttcgag gtcgtgaccc ttgatgatgc cgcctccacc gccgatatcg tcgtgacgac caccggcaac aaggacgtca tcacgatcga ccatatgcgc aagatgaaag acatgtgcat cgtcggcaat atcggccact tcgacaatga aattcaggtc gcagccctgc gcaacctgaa atggaccaat gtgaagccgc aggtcgatct gatcgagttc cccgacggca agcgcctcat ccttctttcg gaaggccgcc tgctcaatct gggcaatgcg acgggccatc caagctttgt catgtcggct tccttcacca atcaggtgtt gggccagatc gaacttttca cccgcaccga tgcctataag aacgaagtct atgtgctgcc gaagcacctt gatgaaaagg tcgctcgtct tcatctcgac aagcttggcg caaagctgac ggtactttcc gaggaacagg ctgcctatat cggcgtcaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for BrabA.00032.a.A3.GE28021
| HARVESTED ON: | 12/7/2009 |
| SEQUENCED ON: | 12/23/2009 |
| EXPECTED MW: | 52kDa |
| OBSERVED MW: | 52kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSQDFVVKD ISLADWGRKE LDIAETEMPG LMAAREEFGK SQPLKGARIS GSLHMTIQTA VLIETLKVLG AEVRWASCNI FSTQDHAAAA IAATGTPVFA VKGETLEEYW TYTDQIFQWP DGEPSNMILD DGGDATMYIL IGARAEAGED VLSNPQSEEE EVLFAQIKKR MAATPGFFTK QRAAIKGVTE ETTTGVNRLY QLQKKGLLPF PAINVNDSVT KSKFDNKYGC KESLVDGIRR GTDVMMAGKV AVVCGYGDVG KGSAQSLAGA GARVKVTEVD PICALQAAMD GFEVVTLDDA ASTADIVVTT TGNKDVITID HMRKMKDMCI VGNIGHFDNE IQVAALRNLK WTNVKPQVDL IEFPDGKRLI LLSEGRLLNL GNATGHPSFV MSASFTNQVL GQIELFTRTD AYKNEVYVLP KHLDEKVARL HLDKLGAKLT VLSEEQAAYI GVTPQGPFKS EHYRY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagccaagat tttgttgtca aggatattag cctggccgat tggggccgca aggaactcga catcgcagaa accgaaatgc cgggcctgat ggctgcccgc gaggaattcg gcaagtcgca gccgctgaag ggcgcgcgca tttccggctc gctgcacatg accattcaaa ctgccgttct cattgagacg ctgaaggttc tgggtgccga agtgcgctgg gcttcctgca acattttctc cacgcaggat cacgcggcgg cggcgattgc ggcaaccggc accccggttt tcgccgtcaa gggcgagacg cttgaagaat actggaccta taccgaccag atcttccagt ggccggatgg cgagccgtcc aacatgatcc tcgatgatgg cggcgacgcc accatgtata ttctcatcgg cgcgcgcgcc gaggctggcg aggacgtcct gtccaatccg cagagcgagg aagaagaagt tcttttcgcg cagatcaaga agcgcatggc cgcaacgccg ggcttcttca cgaagcagcg cgccgcaatc aagggcgtga cggaagaaac gaccaccggc gtcaaccgcc tctatcagtt gcagaagaag ggtctcctgc ccttccccgc catcaacgtc aatgacagtg tgaccaagtc gaagttcgac aacaagtatg gctgcaagga atcgctggtg gatggcattc gtcgcggcac cgacgtcatg atggccggca aggttgccgt cgtctgcggc tatggtgatg tcggcaaagg ctctgcccag tcgctcgccg gtgccggcgc tcgcgtcaag gtcacggaag tcgatccgat ctgcgcgctt caggccgcga tggacggctt cgaggtcgtg acccttgatg atgccgcctc caccgccgat atcgtcgtga cgaccaccgg caacaaggac gtcatcacga tcgaccatat gcgcaagatg aaagacatgt gcatcgtcgg caatatcggc cacttcgaca atgaaattca ggtcgcagcc ctgcgcaacc tgaaatggac caatgtgaag ccgcaggtcg atctgatcga gttccccgac ggcaagcgcc tcatccttct ttcggaaggc cgcctgctca atctgggcaa tgcgacgggc catccaagct ttgtcatgtc ggcttccttc accaatcagg tgttgggcca gatcgaactt ttcacccgca ccgatgccta taagaacgaa gtctatgtgc tgccgaagca ccttgatgaa aaggtcgctc gtcttcatct cgacaagctt ggcgcaaagc tgacggtact ttccgaggaa caggctgcct atatcggcgt cactccacag ggtccgttca agtcggaaca ctacaggtat aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for BrabA.00032.a.A4.GE28022
| HARVESTED ON: | 12/7/2009 |
| SEQUENCED ON: | 12/23/2009 |
| EXPECTED MW: | 51kDa |
| OBSERVED MW: | 52kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 92 |
| PERCENT COVERAGE: | 48 |
Validated AA Sequence
MXTLEAQTQG PGSMSQDFVV KDISLADWGR KELDIAETEM PGLMAAREEF GKSQPLKGAR ISGSLHMTIQ TAVLIETLKV LGAEVRWASC NIFSTQDHAA AAIAATGTPV FAVKGETLEE YWTYTDQIFQ WPDGEPSNMI LDDGGDATMY ILIGARAEAG EDVLSNPQSE EEEVLFAXIK KRMAATPGFF TKQRAAXKGV TEETTTGVXP PLSVAEEGSP ALPRXQRQ
Validated NT Sequence
annncnatna ccatnatatg gntaccctgg aagctcagac ccagggtcct ggttcgatga gccaagattt tgttgtcaag gatattagcc tggccgattg gggccgcaag gaactcgaca tcgcagaaac cgaaatgccg ggcctgatgg ctgcccgcga ggaattcggc aagtcgcagc cgctgaaggg cgcgcgcatt tccggctcgc tgcacatgac cattcaaact gccgttctca ttgagacgct gaaggttctg ggtgccgaag tgcgctgggc ttcctgcaac attttctcca cgcaggatca cgcggcggcg gcgattgcgg caaccggcac cccggttttc gccgtcaagg gcgagacgct tgaagaatac tggacctata ccgaccagat cttccagtgg ccggatggcg agccgtccaa catgatcctc gatgatggcg gcgacgccac catgtatatt ctcatcggcg cgcgcgccga ggctggcgag gacgtcctgt ccaatccgca gagcgaggaa gaagaagttc ttttcgcgca natcaagaag cgcatggccg caacgccggg cttcttcacg aagcagcgcg ccgcanncaa gggcgtgacg gaagaaacga ccaccggcgt caanccgcct ctatcagttg cagaagaagg gtctcctgcc cttccccgcc ntcaacgtca atgacagtgt gancaagtcg aagttcgaca acaagtatgg ctgcnnggaa tcngctggng gnatggnatt cgtcgcgnnn ncgantcnng nntggncggn aagggttgcc gncntcngcg nntatggnga tgncgnnnag gnntctngcn cagtcnntng nnngnngccn nngctcgann caaggnnnnn gnannnnnat ccgnatcngn ncnntnnngg nnnnnatggn nngntnnnaa gnnnnnnncn ntngatgnng nnnnncnncc anngnnnnna nnnnnnnnnn nnnnnggnnn nnnngnnnnn nnnnnnnann gnnnnnnncg nnnnnnnnnn gnnnnannnn nnnnnnnnnn cngcnnnnnn nnnnnantaa nnnnnnaann nnnnnnnnnn nnannncnng nnnnnnnnng nnnnngnnnn nngnnanng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSQDFVVKD ISLADWGRKE LDIAETEMPG LMAAREEFGK SQPLKGARIS GSLHMTIQTA VLIETLKVLG AEVRWASCNI FSTQDHAAAA IAATGTPVFA VKGETLEEYW TYTDQIFQWP DGEPSNMILD DGGDATMYIL IGARAEAGED VLSNPQSEEE EVLFAQIKKR MAATPGFFTK QRAAIKGVTE ETTTGVNRLY QLQKKGLLPF PAINVNDSVT KSKFDNKYGC KESLVDGIRR GTDVMMAGKV AVVCGYGDVG KGSAQSLAGA GARVKVTEVD PICALQAAMD GFEVVTLDDA ASTADIVVTT TGNKDVITID HMRKMKDMCI VGNIGHFDNE IQVAALRNLK WTNVKPQVDL IEFPDGKRLI LLSEGRLLNL GNATGHPSFV MSASFTNQVL GQIELFTRTD AYKNEVYVLP KHLDEKVARL HLDKLGAKLT VLSEEQAAYI GV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagccaagat tttgttgtca aggatattag cctggccgat tggggccgca aggaactcga catcgcagaa accgaaatgc cgggcctgat ggctgcccgc gaggaattcg gcaagtcgca gccgctgaag ggcgcgcgca tttccggctc gctgcacatg accattcaaa ctgccgttct cattgagacg ctgaaggttc tgggtgccga agtgcgctgg gcttcctgca acattttctc cacgcaggat cacgcggcgg cggcgattgc ggcaaccggc accccggttt tcgccgtcaa gggcgagacg cttgaagaat actggaccta taccgaccag atcttccagt ggccggatgg cgagccgtcc aacatgatcc tcgatgatgg cggcgacgcc accatgtata ttctcatcgg cgcgcgcgcc gaggctggcg aggacgtcct gtccaatccg cagagcgagg aagaagaagt tcttttcgcg cagatcaaga agcgcatggc cgcaacgccg ggcttcttca cgaagcagcg cgccgcaatc aagggcgtga cggaagaaac gaccaccggc gtcaaccgcc tctatcagtt gcagaagaag ggtctcctgc ccttccccgc catcaacgtc aatgacagtg tgaccaagtc gaagttcgac aacaagtatg gctgcaagga atcgctggtg gatggcattc gtcgcggcac cgacgtcatg atggccggca aggttgccgt cgtctgcggc tatggtgatg tcggcaaagg ctctgcccag tcgctcgccg gtgccggcgc tcgcgtcaag gtcacggaag tcgatccgat ctgcgcgctt caggccgcga tggacggctt cgaggtcgtg acccttgatg atgccgcctc caccgccgat atcgtcgtga cgaccaccgg caacaaggac gtcatcacga tcgaccatat gcgcaagatg aaagacatgt gcatcgtcgg caatatcggc cacttcgaca atgaaattca ggtcgcagcc ctgcgcaacc tgaaatggac caatgtgaag ccgcaggtcg atctgatcga gttccccgac ggcaagcgcc tcatccttct ttcggaaggc cgcctgctca atctgggcaa tgcgacgggc catccaagct ttgtcatgtc ggcttccttc accaatcagg tgttgggcca gatcgaactt ttcacccgca ccgatgccta taagaacgaa gtctatgtgc tgccgaagca ccttgatgaa aaggtcgctc gtcttcatct cgacaagctt ggcgcaaagc tgacggtact ttccgaggaa caggctgcct atatcggcgt caaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for BrabA.00032.a.A5.GE28023
| HARVESTED ON: | 12/7/2009 |
| SEQUENCED ON: | 12/23/2009 |
| EXPECTED MW: | 52kDa |
| OBSERVED MW: | 52kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVVKDISLA DWGRKELDIA ETEMPGLMAA REEFGKSQPL KGARISGSLH MTIQTAVLIE TLKVLGAEVR WASCNIFSTQ DHAAAAIAAT GTPVFAVKGE TLEEYWTYTD QIFQWPDGEP SNMILDDGGD ATMYILIGAR AEAGEDVLSN PQSEEEEVLF AQIKKRMAAT PGFFTKQRAA IKGVTEETTT GVNRLYQLQK KGLLPFPAIN VNDSVTKSKF DNKYGCKESL VDGIRRGTDV MMAGKVAVVC GYGDVGKGSA QSLAGAGARV KVTEVDPICA LQAAMDGFEV VTLDDAASTA DIVVTTTGNK DVITIDHMRK MKDMCIVGNI GHFDNEIQVA ALRNLKWTNV KPQVDLIEFP DGKRLILLSE GRLLNLGNAT GHPSFVMSAS FTNQVLGQIE LFTRTDAYKN EVYVLPKHLD EKVARLHLDK LGAKLTVLSE EQAAYIGVTP QGPFKSEHYR Y
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggttgtcaag gatattagcc tggccgattg gggccgcaag gaactcgaca tcgcagaaac cgaaatgccg ggcctgatgg ctgcccgcga ggaattcggc aagtcgcagc cgctgaaggg cgcgcgcatt tccggctcgc tgcacatgac cattcaaact gccgttctca ttgagacgct gaaggttctg ggtgccgaag tgcgctgggc ttcctgcaac attttctcca cgcaggatca cgcggcggcg gcgattgcgg caaccggcac cccggttttc gccgtcaagg gcgagacgct tgaagaatac tggacctata ccgaccagat cttccagtgg ccggatggcg agccgtccaa catgatcctc gatgatggcg gcgacgccac catgtatatt ctcatcggcg cgcgcgccga ggctggcgag gacgtcctgt ccaatccgca gagcgaggaa gaagaagttc ttttcgcgca gatcaagaag cgcatggccg caacgccggg cttcttcacg aagcagcgcg ccgcaatcaa gggcgtgacg gaagaaacga ccaccggcgt caaccgcctc tatcagttgc agaagaaggg tctcctgccc ttccccgcca tcaacgtcaa tgacagtgtg accaagtcga agttcgacaa caagtatggc tgcaaggaat cgctggtgga tggcattcgt cgcggcaccg acgtcatgat ggccggcaag gttgccgtcg tctgcggcta tggtgatgtc ggcaaaggct ctgcccagtc gctcgccggt gccggcgctc gcgtcaaggt cacggaagtc gatccgatct gcgcgcttca ggccgcgatg gacggcttcg aggtcgtgac ccttgatgat gccgcctcca ccgccgatat cgtcgtgacg accaccggca acaaggacgt catcacgatc gaccatatgc gcaagatgaa agacatgtgc atcgtcggca atatcggcca cttcgacaat gaaattcagg tcgcagccct gcgcaacctg aaatggacca atgtgaagcc gcaggtcgat ctgatcgagt tccccgacgg caagcgcctc atccttcttt cggaaggccg cctgctcaat ctgggcaatg cgacgggcca tccaagcttt gtcatgtcgg cttccttcac caatcaggtg ttgggccaga tcgaactttt cacccgcacc gatgcctata agaacgaagt ctatgtgctg ccgaagcacc ttgatgaaaa ggtcgctcgt cttcatctcg acaagcttgg cgcaaagctg acggtacttt ccgaggaaca ggctgcctat atcggcgtca ctccacaggg tccgttcaag tcggaacact acaggtataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for BrabA.00032.a.A6.GE28024
| HARVESTED ON: | 12/7/2009 |
| SEQUENCED ON: | 12/23/2009 |
| EXPECTED MW: | 50kDa |
| OBSERVED MW: | 51kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 95 |
Validated AA Sequence
MVVKDISLAD WGRKELDIAE TEMPGLMAAR EEFGKSQPLK GARISGSLHM TIQTAVLIET LKVLGAEVRW ASCNIFSTQD HAAAAIAATG TPVFAVKGET LEEYWTYTDQ IFQWPDGEPS NMILDDGGDA TMYILIGARA EAGEDVLSNP QSEEEEVLFA QIKKRMAATP GFFTKQRAAI KGVTEETTTG VNRLYQLQKK GLLPFPAINV NDSVTKSKFD NKYGCKESLV DGIRRGTDVM MAGKVAVVCG YGDVGKGSAQ SLAGAGARVK VTEVDPICAL QAAMDGFEVV TLDDAASTAD IVVTTTGNKD VITIDHMRKM KDMCIVGNIG HFDNEIQVAA LRNLKWTNVK PQVDLIEFPD GKRLILLSEG RLLNLGNATG HPSFVMSASF TNQVLGQIEL FTRTDAYKNE VYVLPKHLDE KVARLHLDKL GAKLTVLSEE QAAYI
Validated NT Sequence
cgatataggc agcctgttcc tcggaaagta ccgtcagctt tgcgccaagc ttgtcgagat gaagacgagc gaccttttca tcaaggtgct tcggcagcac atagacttcg ttcttatagg catcggtgcg ggtgaaaagt tcgatctggc ccaacacctg attggtgaag gaagccgaca tgacaaagct tggatggccc gtcgcattgc ccagattgag caggcggcct tccgaaagaa ggatgaggcg cttgccgtcg gggaactcga tcagatcgac ctgcggcttc acattggtcc atttcaggtt gcgcagggct gcgacctgaa tttcattgtc gaagtggccg atattgccga cgatgcacat gtctttcatc ttgcgcatat ggtcgatcgt gatgacgtcc ttgttgccgg tggtcgtcac gacgatatcg gcggtggagg cggcatcatc aagggtcacg acctcgaagc cgtccatcgc ggcctgaagc gcgcagatcg gatcgacttc cgtgaccttg acgcgagcgc cggcaccggc gagcgactgg gcagagcctt tgccgacatc accatagccg cagacgacgg caaccttgcc ggccatcatg acgtcggtgc cgcgacgaat gccatccacc agcgattcct tgcagccata cttgttgtcg aacttcgact tggtcacact gtcattgacg ttgatggcgg ggaagggcag gagacccttc ttctgcaact gatagaggcg gttgacgccg gtggtcgttt cttccgtcac gcccttgatt gcggcgcgct gcttcgtgaa gaagcccggc gttgcggcca tgcgcttctt gatctgcgcg aaaagaactt cttcttcctc gctctgcgga ttggacagga cgtcctcgcc agcctcggcg cgcgcgccga tgagaatata catggtggcg tcgccgccat catcgaggat catgttggac ggctcgccat ccggccactg gaagatctgg tcggtatagg tccagtattc ttcaagcgtc tcgcccttga cggcgaaaac cggggtgccg gttgccgcaa tcgccgccgc cgcgtgatcc tgcgtggaga aaatgttgca ggaagcccag cgcacttcgg cacccagaac cttcagcgtc tcaatgagaa cggcagtttg aatggtcatg tgcagcgagc cggaaatgcg cgcgcccttc agcggctgcg acttgccgaa ttcctcgcgg gcagccatca ggcccggcat ttcggtttct gcgatgtcga gttccttgcg gccccaatcg gccaggctaa tatccttgac aaccatcgaa ccaggaccct gggt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVVKDISLA DWGRKELDIA ETEMPGLMAA REEFGKSQPL KGARISGSLH MTIQTAVLIE TLKVLGAEVR WASCNIFSTQ DHAAAAIAAT GTPVFAVKGE TLEEYWTYTD QIFQWPDGEP SNMILDDGGD ATMYILIGAR AEAGEDVLSN PQSEEEEVLF AQIKKRMAAT PGFFTKQRAA IKGVTEETTT GVNRLYQLQK KGLLPFPAIN VNDSVTKSKF DNKYGCKESL VDGIRRGTDV MMAGKVAVVC GYGDVGKGSA QSLAGAGARV KVTEVDPICA LQAAMDGFEV VTLDDAASTA DIVVTTTGNK DVITIDHMRK MKDMCIVGNI GHFDNEIQVA ALRNLKWTNV KPQVDLIEFP DGKRLILLSE GRLLNLGNAT GHPSFVMSAS FTNQVLGQIE LFTRTDAYKN EVYVLPKHLD EKVARLHLDK LGAKLTVLSE EQAAYIGV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggttgtcaag gatattagcc tggccgattg gggccgcaag gaactcgaca tcgcagaaac cgaaatgccg ggcctgatgg ctgcccgcga ggaattcggc aagtcgcagc cgctgaaggg cgcgcgcatt tccggctcgc tgcacatgac cattcaaact gccgttctca ttgagacgct gaaggttctg ggtgccgaag tgcgctgggc ttcctgcaac attttctcca cgcaggatca cgcggcggcg gcgattgcgg caaccggcac cccggttttc gccgtcaagg gcgagacgct tgaagaatac tggacctata ccgaccagat cttccagtgg ccggatggcg agccgtccaa catgatcctc gatgatggcg gcgacgccac catgtatatt ctcatcggcg cgcgcgccga ggctggcgag gacgtcctgt ccaatccgca gagcgaggaa gaagaagttc ttttcgcgca gatcaagaag cgcatggccg caacgccggg cttcttcacg aagcagcgcg ccgcaatcaa gggcgtgacg gaagaaacga ccaccggcgt caaccgcctc tatcagttgc agaagaaggg tctcctgccc ttccccgcca tcaacgtcaa tgacagtgtg accaagtcga agttcgacaa caagtatggc tgcaaggaat cgctggtgga tggcattcgt cgcggcaccg acgtcatgat ggccggcaag gttgccgtcg tctgcggcta tggtgatgtc ggcaaaggct ctgcccagtc gctcgccggt gccggcgctc gcgtcaaggt cacggaagtc gatccgatct gcgcgcttca ggccgcgatg gacggcttcg aggtcgtgac ccttgatgat gccgcctcca ccgccgatat cgtcgtgacg accaccggca acaaggacgt catcacgatc gaccatatgc gcaagatgaa agacatgtgc atcgtcggca atatcggcca cttcgacaat gaaattcagg tcgcagccct gcgcaacctg aaatggacca atgtgaagcc gcaggtcgat ctgatcgagt tccccgacgg caagcgcctc atccttcttt cggaaggccg cctgctcaat ctgggcaatg cgacgggcca tccaagcttt gtcatgtcgg cttccttcac caatcaggtg ttgggccaga tcgaactttt cacccgcacc gatgcctata agaacgaagt ctatgtgctg ccgaagcacc ttgatgaaaa ggtcgctcgt cttcatctcg acaagcttgg cgcaaagctg acggtacttt ccgaggaaca ggctgcctat atcggcgtca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for BrabA.00032.a.A2.PS00516
| PURIFICATION DATe: | 3/12/2010 |
| CONCENTRATION: | 99.05mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 10 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 96 |
| PERCENT COVERAGE: | 59 |
Protocol Notes
notes unavailable
Validated AA Sequence
MTASQDFVVK DISLADWGRK ELDIAETEMP GLMAAREEFG KSQPLKGARI SGSLHMTIQT AVLIETLKVL GAEVRWASCN IFSTQDHAAA AIAATGTPVF AVKGETLEEY WTYTDQIFQW PDGEPSNMIL DDGGDATMYI LIGARAEAGE DVLSNPQSEE EEVLFAQIKK RMAATPGFFT KQRAAIKGVX EETTTGVNRL YQLQKKGLLP FPAINVNDSV XKSKFDXXYG CXESLXDGXR RGTDVMMAGK VAVVCGYGDV GKXSAQSLAG AGARVKVTEX X
Validated NT Sequence
aagctcagac ccagggtcct ggttcgatga ccgcaagcca agattttgtt gtcaaggata ttagcctggc cgattggggc cgcaaggaac tcgacatcgc agaaaccgaa atgccgggcc tgatggctgc ccgcgaggaa ttcggcaagt cgcagccgct gaagggcgcg cgcatttccg gctcgctgca catgaccatt caaactgccg ttctcattga gacgctgaag gttctgggtg ccgaagtgcg ctgggcttcc tgcaacattt tctccacgca ggatcacgcg gcggcggcga ttgcggcaac cggcaccccg gttttcgccg tcaagggcga gacgcttgaa gaatactgga cctataccga ccagatcttc cagtggccgg atggcgagcc gtccaacatg atcctcgatg atggcggcga cgccaccatg tatattctca tcggcgcgcg cgccgaggct ggcgaggacg tcctgtccaa tccgcagagc gaggaagaag aagttctttt cgcgcagatc aagaagcgca tggccgcaac gccgggcttc ttcacgaagc agcgcgccgc aatcaagggc gtgncggaag aaacgaccac cggcgtcaac cgcctctatc agttgcagaa gaagggtctc ctgcccttcc ccgccatcaa cgtcaatgac agtgtganca agtcgaagtt cgacnacnag tatggctgcn aggaatcgct ggnggatggc nttcgtcgcg gcaccgacgt catgatggcc ggnaaggttg ccgtcgtctg cggctatggt gatgtcggca aangntctgc ccagtcgctc gccggngccg gcgctcgcgt caaggtcacg gaantcnatc c
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTASQDFVV KDISLADWGR KELDIAETEM PGLMAAREEF GKSQPLKGAR ISGSLHMTIQ TAVLIETLKV LGAEVRWASC NIFSTQDHAA AAIAATGTPV FAVKGETLEE YWTYTDQIFQ WPDGEPSNMI LDDGGDATMY ILIGARAEAG EDVLSNPQSE EEEVLFAQIK KRMAATPGFF TKQRAAIKGV TEETTTGVNR LYQLQKKGLL PFPAINVNDS VTKSKFDNKY GCKESLVDGI RRGTDVMMAG KVAVVCGYGD VGKGSAQSLA GAGARVKVTE VDPICALQAA MDGFEVVTLD DAASTADIVV TTTGNKDVIT IDHMRKMKDM CIVGNIGHFD NEIQVAALRN LKWTNVKPQV DLIEFPDGKR LILLSEGRLL NLGNATGHPS FVMSASFTNQ VLGQIELFTR TDAYKNEVYV LPKHLDEKVA RLHLDKLGAK LTVLSEEQAA YIGV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCGCAAGC CAAGATTTTG TTGTCAAGGA TATTAGCCTG GCCGATTGGG GCCGCAAGGA ACTCGACATC GCAGAAACCG AAATGCCGGG CCTGATGGCT GCCCGCGAGG AATTCGGCAA GTCGCAGCCG CTGAAGGGCG CGCGCATTTC CGGCTCGCTG CACATGACCA TTCAAACTGC CGTTCTCATT GAGACGCTGA AGGTTCTGGG TGCCGAAGTG CGCTGGGCTT CCTGCAACAT TTTCTCCACG CAGGATCACG CGGCGGCGGC GATTGCGGCA ACCGGCACCC CGGTTTTCGC CGTCAAGGGC GAGACGCTTG AAGAATACTG GACCTATACC GACCAGATCT TCCAGTGGCC GGATGGCGAG CCGTCCAACA TGATCCTCGA TGATGGCGGC GACGCCACCA TGTATATTCT CATCGGCGCG CGCGCCGAGG CTGGCGAGGA CGTCCTGTCC AATCCGCAGA GCGAGGAAGA AGAAGTTCTT TTCGCGCAGA TCAAGAAGCG CATGGCCGCA ACGCCGGGCT TCTTCACGAA GCAGCGCGCC GCAATCAAGG GCGTGACGGA AGAAACGACC ACCGGCGTCA ACCGCCTCTA TCAGTTGCAG AAGAAGGGTC TCCTGCCCTT CCCCGCCATC AACGTCAATG ACAGTGTGAC CAAGTCGAAG TTCGACAACA AGTATGGCTG CAAGGAATCG CTGGTGGATG GCATTCGTCG CGGCACCGAC GTCATGATGG CCGGCAAGGT TGCCGTCGTC TGCGGCTATG GTGATGTCGG CAAAGGCTCT GCCCAGTCGC TCGCCGGTGC CGGCGCTCGC GTCAAGGTCA CGGAAGTCGA TCCGATCTGC GCGCTTCAGG CCGCGATGGA CGGCTTCGAG GTCGTGACCC TTGATGATGC CGCCTCCACC GCCGATATCG TCGTGACGAC CACCGGCAAC AAGGACGTCA TCACGATCGA CCATATGCGC AAGATGAAAG ACATGTGCAT CGTCGGCAAT ATCGGCCACT TCGACAATGA AATTCAGGTC GCAGCCCTGC GCAACCTGAA ATGGACCAAT GTGAAGCCGC AGGTCGATCT GATCGAGTTC CCCGACGGCA AGCGCCTCAT CCTTCTTTCG GAAGGCCGCC TGCTCAATCT GGGCAATGCG ACGGGCCATC CAAGCTTTGT CATGTCGGCT TCCTTCACCA ATCAGGTGTT GGGCCAGATC GAACTTTTCA CCCGCACCGA TGCCTATAAG AACGAAGTCT ATGTGCTGCC GAAGCACCTT GATGAAAAGG TCGCTCGTCT TCATCTCGAC AAGCTTGGCG CAAAGCTGAC GGTACTTTCC GAGGAACAGG CTGCCTATAT CGGCGTCAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA
Details for BrabA.00032.a.A3.PS00530
| PURIFICATION DATe: | 3/11/2010 |
| CONCENTRATION: | 18.1mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSQDFVVKD ISLADWGRKE LDIAETEMPG LMAAREEFGK SQPLKGARIS GSLHMTIQTA VLIETLKVLG AEVRWASCNI FSTQDHAAAA IAATGTPVFA VKGETLEEYW TYTDQIFQWP DGEPSNMILD DGGDATMYIL IGARAEAGED VLSNPQSEEE EVLFAQIKKR MAATPGFFTK QRAAIKGVTE ETTTGVNRLY QLQKKGLLPF PAINVNDSVT KSKFDNKYGC KESLVDGIRR GTDVMMAGKV AVVCGYGDVG KGSAQSLAGA GARVKVTEVD PICALQAAMD GFEVVTLDDA ASTADIVVTT TGNKDVITID HMRKMKDMCI VGNIGHFDNE IQVAALRNLK WTNVKPQVDL IEFPDGKRLI LLSEGRLLNL GNATGHPSFV MSASFTNQVL GQIELFTRTD AYKNEVYVLP KHLDEKVARL HLDKLGAKLT VLSEEQAAYI GVTPQGPFKS EHYRY
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCCAAGAT TTTGTTGTCA AGGATATTAG CCTGGCCGAT TGGGGCCGCA AGGAACTCGA CATCGCAGAA ACCGAAATGC CGGGCCTGAT GGCTGCCCGC GAGGAATTCG GCAAGTCGCA GCCGCTGAAG GGCGCGCGCA TTTCCGGCTC GCTGCACATG ACCATTCAAA CTGCCGTTCT CATTGAGACG CTGAAGGTTC TGGGTGCCGA AGTGCGCTGG GCTTCCTGCA ACATTTTCTC CACGCAGGAT CACGCGGCGG CGGCGATTGC GGCAACCGGC ACCCCGGTTT TCGCCGTCAA GGGCGAGACG CTTGAAGAAT ACTGGACCTA TACCGACCAG ATCTTCCAGT GGCCGGATGG CGAGCCGTCC AACATGATCC TCGATGATGG CGGCGACGCC ACCATGTATA TTCTCATCGG CGCGCGCGCC GAGGCTGGCG AGGACGTCCT GTCCAATCCG CAGAGCGAGG AAGAAGAAGT TCTTTTCGCG CAGATCAAGA AGCGCATGGC CGCAACGCCG GGCTTCTTCA CGAAGCAGCG CGCCGCAATC AAGGGCGTGA CGGAAGAAAC GACCACCGGC GTCAACCGCC TCTATCAGTT GCAGAAGAAG GGTCTCCTGC CCTTCCCCGC CATCAACGTC AATGACAGTG TGACCAAGTC GAAGTTCGAC AACAAGTATG GCTGCAAGGA ATCGCTGGTG GATGGCATTC GTCGCGGCAC CGACGTCATG ATGGCCGGCA AGGTTGCCGT CGTCTGCGGC TATGGTGATG TCGGCAAAGG CTCTGCCCAG TCGCTCGCCG GTGCCGGCGC TCGCGTCAAG GTCACGGAAG TCGATCCGAT CTGCGCGCTT CAGGCCGCGA TGGACGGCTT CGAGGTCGTG ACCCTTGATG ATGCCGCCTC CACCGCCGAT ATCGTCGTGA CGACCACCGG CAACAAGGAC GTCATCACGA TCGACCATAT GCGCAAGATG AAAGACATGT GCATCGTCGG CAATATCGGC CACTTCGACA ATGAAATTCA GGTCGCAGCC CTGCGCAACC TGAAATGGAC CAATGTGAAG CCGCAGGTCG ATCTGATCGA GTTCCCCGAC GGCAAGCGCC TCATCCTTCT TTCGGAAGGC CGCCTGCTCA ATCTGGGCAA TGCGACGGGC CATCCAAGCT TTGTCATGTC GGCTTCCTTC ACCAATCAGG TGTTGGGCCA GATCGAACTT TTCACCCGCA CCGATGCCTA TAAGAACGAA GTCTATGTGC TGCCGAAGCA CCTTGATGAA AAGGTCGCTC GTCTTCATCT CGACAAGCTT GGCGCAAAGC TGACGGTACT TTCCGAGGAA CAGGCTGCCT ATATCGGCGT CACTCCACAG GGTCCGTTCA AGTCGGAACA CTACAGGTAT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA
Details for BrabA.00032.a.A4.PS00520
| PURIFICATION DATe: | 3/11/2010 |
| CONCENTRATION: | 22.35mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 92 |
| PERCENT COVERAGE: | 48 |
Protocol Notes
notes unavailable
Validated AA Sequence
MXTLEAQTQG PGSMSQDFVV KDISLADWGR KELDIAETEM PGLMAAREEF GKSQPLKGAR ISGSLHMTIQ TAVLIETLKV LGAEVRWASC NIFSTQDHAA AAIAATGTPV FAVKGETLEE YWTYTDQIFQ WPDGEPSNMI LDDGGDATMY ILIGARAEAG EDVLSNPQSE EEEVLFAXIK KRMAATPGFF TKQRAAXKGV TEETTTGVXP PLSVAEEGSP ALPRXQRQ
Validated NT Sequence
annncnatna ccatnatatg gntaccctgg aagctcagac ccagggtcct ggttcgatga gccaagattt tgttgtcaag gatattagcc tggccgattg gggccgcaag gaactcgaca tcgcagaaac cgaaatgccg ggcctgatgg ctgcccgcga ggaattcggc aagtcgcagc cgctgaaggg cgcgcgcatt tccggctcgc tgcacatgac cattcaaact gccgttctca ttgagacgct gaaggttctg ggtgccgaag tgcgctgggc ttcctgcaac attttctcca cgcaggatca cgcggcggcg gcgattgcgg caaccggcac cccggttttc gccgtcaagg gcgagacgct tgaagaatac tggacctata ccgaccagat cttccagtgg ccggatggcg agccgtccaa catgatcctc gatgatggcg gcgacgccac catgtatatt ctcatcggcg cgcgcgccga ggctggcgag gacgtcctgt ccaatccgca gagcgaggaa gaagaagttc ttttcgcgca natcaagaag cgcatggccg caacgccggg cttcttcacg aagcagcgcg ccgcanncaa gggcgtgacg gaagaaacga ccaccggcgt caanccgcct ctatcagttg cagaagaagg gtctcctgcc cttccccgcc ntcaacgtca atgacagtgt gancaagtcg aagttcgaca acaagtatgg ctgcnnggaa tcngctggng gnatggnatt cgtcgcgnnn ncgantcnng nntggncggn aagggttgcc gncntcngcg nntatggnga tgncgnnnag gnntctngcn cagtcnntng nnngnngccn nngctcgann caaggnnnnn gnannnnnat ccgnatcngn ncnntnnngg nnnnnatggn nngntnnnaa gnnnnnnncn ntngatgnng nnnnncnncc anngnnnnna nnnnnnnnnn nnnnnggnnn nnnngnnnnn nnnnnnnann gnnnnnnncg nnnnnnnnnn gnnnnannnn nnnnnnnnnn cngcnnnnnn nnnnnantaa nnnnnnaann nnnnnnnnnn nnannncnng nnnnnnnnng nnnnngnnnn nngnnanng
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSQDFVVKD ISLADWGRKE LDIAETEMPG LMAAREEFGK SQPLKGARIS GSLHMTIQTA VLIETLKVLG AEVRWASCNI FSTQDHAAAA IAATGTPVFA VKGETLEEYW TYTDQIFQWP DGEPSNMILD DGGDATMYIL IGARAEAGED VLSNPQSEEE EVLFAQIKKR MAATPGFFTK QRAAIKGVTE ETTTGVNRLY QLQKKGLLPF PAINVNDSVT KSKFDNKYGC KESLVDGIRR GTDVMMAGKV AVVCGYGDVG KGSAQSLAGA GARVKVTEVD PICALQAAMD GFEVVTLDDA ASTADIVVTT TGNKDVITID HMRKMKDMCI VGNIGHFDNE IQVAALRNLK WTNVKPQVDL IEFPDGKRLI LLSEGRLLNL GNATGHPSFV MSASFTNQVL GQIELFTRTD AYKNEVYVLP KHLDEKVARL HLDKLGAKLT VLSEEQAAYI GV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCCAAGAT TTTGTTGTCA AGGATATTAG CCTGGCCGAT TGGGGCCGCA AGGAACTCGA CATCGCAGAA ACCGAAATGC CGGGCCTGAT GGCTGCCCGC GAGGAATTCG GCAAGTCGCA GCCGCTGAAG GGCGCGCGCA TTTCCGGCTC GCTGCACATG ACCATTCAAA CTGCCGTTCT CATTGAGACG CTGAAGGTTC TGGGTGCCGA AGTGCGCTGG GCTTCCTGCA ACATTTTCTC CACGCAGGAT CACGCGGCGG CGGCGATTGC GGCAACCGGC ACCCCGGTTT TCGCCGTCAA GGGCGAGACG CTTGAAGAAT ACTGGACCTA TACCGACCAG ATCTTCCAGT GGCCGGATGG CGAGCCGTCC AACATGATCC TCGATGATGG CGGCGACGCC ACCATGTATA TTCTCATCGG CGCGCGCGCC GAGGCTGGCG AGGACGTCCT GTCCAATCCG CAGAGCGAGG AAGAAGAAGT TCTTTTCGCG CAGATCAAGA AGCGCATGGC CGCAACGCCG GGCTTCTTCA CGAAGCAGCG CGCCGCAATC AAGGGCGTGA CGGAAGAAAC GACCACCGGC GTCAACCGCC TCTATCAGTT GCAGAAGAAG GGTCTCCTGC CCTTCCCCGC CATCAACGTC AATGACAGTG TGACCAAGTC GAAGTTCGAC AACAAGTATG GCTGCAAGGA ATCGCTGGTG GATGGCATTC GTCGCGGCAC CGACGTCATG ATGGCCGGCA AGGTTGCCGT CGTCTGCGGC TATGGTGATG TCGGCAAAGG CTCTGCCCAG TCGCTCGCCG GTGCCGGCGC TCGCGTCAAG GTCACGGAAG TCGATCCGAT CTGCGCGCTT CAGGCCGCGA TGGACGGCTT CGAGGTCGTG ACCCTTGATG ATGCCGCCTC CACCGCCGAT ATCGTCGTGA CGACCACCGG CAACAAGGAC GTCATCACGA TCGACCATAT GCGCAAGATG AAAGACATGT GCATCGTCGG CAATATCGGC CACTTCGACA ATGAAATTCA GGTCGCAGCC CTGCGCAACC TGAAATGGAC CAATGTGAAG CCGCAGGTCG ATCTGATCGA GTTCCCCGAC GGCAAGCGCC TCATCCTTCT TTCGGAAGGC CGCCTGCTCA ATCTGGGCAA TGCGACGGGC CATCCAAGCT TTGTCATGTC GGCTTCCTTC ACCAATCAGG TGTTGGGCCA GATCGAACTT TTCACCCGCA CCGATGCCTA TAAGAACGAA GTCTATGTGC TGCCGAAGCA CCTTGATGAA AAGGTCGCTC GTCTTCATCT CGACAAGCTT GGCGCAAAGC TGACGGTACT TTCCGAGGAA CAGGCTGCCT ATATCGGCGT CAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA
Details for BrabA.00032.a.A5.PS00514
| PURIFICATION DATe: | 3/12/2010 |
| CONCENTRATION: | 79.84mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVVKDISLA DWGRKELDIA ETEMPGLMAA REEFGKSQPL KGARISGSLH MTIQTAVLIE TLKVLGAEVR WASCNIFSTQ DHAAAAIAAT GTPVFAVKGE TLEEYWTYTD QIFQWPDGEP SNMILDDGGD ATMYILIGAR AEAGEDVLSN PQSEEEEVLF AQIKKRMAAT PGFFTKQRAA IKGVTEETTT GVNRLYQLQK KGLLPFPAIN VNDSVTKSKF DNKYGCKESL VDGIRRGTDV MMAGKVAVVC GYGDVGKGSA QSLAGAGARV KVTEVDPICA LQAAMDGFEV VTLDDAASTA DIVVTTTGNK DVITIDHMRK MKDMCIVGNI GHFDNEIQVA ALRNLKWTNV KPQVDLIEFP DGKRLILLSE GRLLNLGNAT GHPSFVMSAS FTNQVLGQIE LFTRTDAYKN EVYVLPKHLD EKVARLHLDK LGAKLTVLSE EQAAYIGVTP QGPFKSEHYR Y
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTTGTCAAG GATATTAGCC TGGCCGATTG GGGCCGCAAG GAACTCGACA TCGCAGAAAC CGAAATGCCG GGCCTGATGG CTGCCCGCGA GGAATTCGGC AAGTCGCAGC CGCTGAAGGG CGCGCGCATT TCCGGCTCGC TGCACATGAC CATTCAAACT GCCGTTCTCA TTGAGACGCT GAAGGTTCTG GGTGCCGAAG TGCGCTGGGC TTCCTGCAAC ATTTTCTCCA CGCAGGATCA CGCGGCGGCG GCGATTGCGG CAACCGGCAC CCCGGTTTTC GCCGTCAAGG GCGAGACGCT TGAAGAATAC TGGACCTATA CCGACCAGAT CTTCCAGTGG CCGGATGGCG AGCCGTCCAA CATGATCCTC GATGATGGCG GCGACGCCAC CATGTATATT CTCATCGGCG CGCGCGCCGA GGCTGGCGAG GACGTCCTGT CCAATCCGCA GAGCGAGGAA GAAGAAGTTC TTTTCGCGCA GATCAAGAAG CGCATGGCCG CAACGCCGGG CTTCTTCACG AAGCAGCGCG CCGCAATCAA GGGCGTGACG GAAGAAACGA CCACCGGCGT CAACCGCCTC TATCAGTTGC AGAAGAAGGG TCTCCTGCCC TTCCCCGCCA TCAACGTCAA TGACAGTGTG ACCAAGTCGA AGTTCGACAA CAAGTATGGC TGCAAGGAAT CGCTGGTGGA TGGCATTCGT CGCGGCACCG ACGTCATGAT GGCCGGCAAG GTTGCCGTCG TCTGCGGCTA TGGTGATGTC GGCAAAGGCT CTGCCCAGTC GCTCGCCGGT GCCGGCGCTC GCGTCAAGGT CACGGAAGTC GATCCGATCT GCGCGCTTCA GGCCGCGATG GACGGCTTCG AGGTCGTGAC CCTTGATGAT GCCGCCTCCA CCGCCGATAT CGTCGTGACG ACCACCGGCA ACAAGGACGT CATCACGATC GACCATATGC GCAAGATGAA AGACATGTGC ATCGTCGGCA ATATCGGCCA CTTCGACAAT GAAATTCAGG TCGCAGCCCT GCGCAACCTG AAATGGACCA ATGTGAAGCC GCAGGTCGAT CTGATCGAGT TCCCCGACGG CAAGCGCCTC ATCCTTCTTT CGGAAGGCCG CCTGCTCAAT CTGGGCAATG CGACGGGCCA TCCAAGCTTT GTCATGTCGG CTTCCTTCAC CAATCAGGTG TTGGGCCAGA TCGAACTTTT CACCCGCACC GATGCCTATA AGAACGAAGT CTATGTGCTG CCGAAGCACC TTGATGAAAA GGTCGCTCGT CTTCATCTCG ACAAGCTTGG CGCAAAGCTG ACGGTACTTT CCGAGGAACA GGCTGCCTAT ATCGGCGTCA CTCCACAGGG TCCGTTCAAG TCGGAACACT ACAGGTATAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA
Details for BrabA.00032.a.A6.PS00517
| PURIFICATION DATe: | 3/12/2010 |
| CONCENTRATION: | 37.41mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 95 |
Protocol Notes
notes unavailable
Validated AA Sequence
MVVKDISLAD WGRKELDIAE TEMPGLMAAR EEFGKSQPLK GARISGSLHM TIQTAVLIET LKVLGAEVRW ASCNIFSTQD HAAAAIAATG TPVFAVKGET LEEYWTYTDQ IFQWPDGEPS NMILDDGGDA TMYILIGARA EAGEDVLSNP QSEEEEVLFA QIKKRMAATP GFFTKQRAAI KGVTEETTTG VNRLYQLQKK GLLPFPAINV NDSVTKSKFD NKYGCKESLV DGIRRGTDVM MAGKVAVVCG YGDVGKGSAQ SLAGAGARVK VTEVDPICAL QAAMDGFEVV TLDDAASTAD IVVTTTGNKD VITIDHMRKM KDMCIVGNIG HFDNEIQVAA LRNLKWTNVK PQVDLIEFPD GKRLILLSEG RLLNLGNATG HPSFVMSASF TNQVLGQIEL FTRTDAYKNE VYVLPKHLDE KVARLHLDKL GAKLTVLSEE QAAYI
Validated NT Sequence
cgatataggc agcctgttcc tcggaaagta ccgtcagctt tgcgccaagc ttgtcgagat gaagacgagc gaccttttca tcaaggtgct tcggcagcac atagacttcg ttcttatagg catcggtgcg ggtgaaaagt tcgatctggc ccaacacctg attggtgaag gaagccgaca tgacaaagct tggatggccc gtcgcattgc ccagattgag caggcggcct tccgaaagaa ggatgaggcg cttgccgtcg gggaactcga tcagatcgac ctgcggcttc acattggtcc atttcaggtt gcgcagggct gcgacctgaa tttcattgtc gaagtggccg atattgccga cgatgcacat gtctttcatc ttgcgcatat ggtcgatcgt gatgacgtcc ttgttgccgg tggtcgtcac gacgatatcg gcggtggagg cggcatcatc aagggtcacg acctcgaagc cgtccatcgc ggcctgaagc gcgcagatcg gatcgacttc cgtgaccttg acgcgagcgc cggcaccggc gagcgactgg gcagagcctt tgccgacatc accatagccg cagacgacgg caaccttgcc ggccatcatg acgtcggtgc cgcgacgaat gccatccacc agcgattcct tgcagccata cttgttgtcg aacttcgact tggtcacact gtcattgacg ttgatggcgg ggaagggcag gagacccttc ttctgcaact gatagaggcg gttgacgccg gtggtcgttt cttccgtcac gcccttgatt gcggcgcgct gcttcgtgaa gaagcccggc gttgcggcca tgcgcttctt gatctgcgcg aaaagaactt cttcttcctc gctctgcgga ttggacagga cgtcctcgcc agcctcggcg cgcgcgccga tgagaatata catggtggcg tcgccgccat catcgaggat catgttggac ggctcgccat ccggccactg gaagatctgg tcggtatagg tccagtattc ttcaagcgtc tcgcccttga cggcgaaaac cggggtgccg gttgccgcaa tcgccgccgc cgcgtgatcc tgcgtggaga aaatgttgca ggaagcccag cgcacttcgg cacccagaac cttcagcgtc tcaatgagaa cggcagtttg aatggtcatg tgcagcgagc cggaaatgcg cgcgcccttc agcggctgcg acttgccgaa ttcctcgcgg gcagccatca ggcccggcat ttcggtttct gcgatgtcga gttccttgcg gccccaatcg gccaggctaa tatccttgac aaccatcgaa ccaggaccct gggt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVVKDISLA DWGRKELDIA ETEMPGLMAA REEFGKSQPL KGARISGSLH MTIQTAVLIE TLKVLGAEVR WASCNIFSTQ DHAAAAIAAT GTPVFAVKGE TLEEYWTYTD QIFQWPDGEP SNMILDDGGD ATMYILIGAR AEAGEDVLSN PQSEEEEVLF AQIKKRMAAT PGFFTKQRAA IKGVTEETTT GVNRLYQLQK KGLLPFPAIN VNDSVTKSKF DNKYGCKESL VDGIRRGTDV MMAGKVAVVC GYGDVGKGSA QSLAGAGARV KVTEVDPICA LQAAMDGFEV VTLDDAASTA DIVVTTTGNK DVITIDHMRK MKDMCIVGNI GHFDNEIQVA ALRNLKWTNV KPQVDLIEFP DGKRLILLSE GRLLNLGNAT GHPSFVMSAS FTNQVLGQIE LFTRTDAYKN EVYVLPKHLD EKVARLHLDK LGAKLTVLSE EQAAYIGV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTTGTCAAG GATATTAGCC TGGCCGATTG GGGCCGCAAG GAACTCGACA TCGCAGAAAC CGAAATGCCG GGCCTGATGG CTGCCCGCGA GGAATTCGGC AAGTCGCAGC CGCTGAAGGG CGCGCGCATT TCCGGCTCGC TGCACATGAC CATTCAAACT GCCGTTCTCA TTGAGACGCT GAAGGTTCTG GGTGCCGAAG TGCGCTGGGC TTCCTGCAAC ATTTTCTCCA CGCAGGATCA CGCGGCGGCG GCGATTGCGG CAACCGGCAC CCCGGTTTTC GCCGTCAAGG GCGAGACGCT TGAAGAATAC TGGACCTATA CCGACCAGAT CTTCCAGTGG CCGGATGGCG AGCCGTCCAA CATGATCCTC GATGATGGCG GCGACGCCAC CATGTATATT CTCATCGGCG CGCGCGCCGA GGCTGGCGAG GACGTCCTGT CCAATCCGCA GAGCGAGGAA GAAGAAGTTC TTTTCGCGCA GATCAAGAAG CGCATGGCCG CAACGCCGGG CTTCTTCACG AAGCAGCGCG CCGCAATCAA GGGCGTGACG GAAGAAACGA CCACCGGCGT CAACCGCCTC TATCAGTTGC AGAAGAAGGG TCTCCTGCCC TTCCCCGCCA TCAACGTCAA TGACAGTGTG ACCAAGTCGA AGTTCGACAA CAAGTATGGC TGCAAGGAAT CGCTGGTGGA TGGCATTCGT CGCGGCACCG ACGTCATGAT GGCCGGCAAG GTTGCCGTCG TCTGCGGCTA TGGTGATGTC GGCAAAGGCT CTGCCCAGTC GCTCGCCGGT GCCGGCGCTC GCGTCAAGGT CACGGAAGTC GATCCGATCT GCGCGCTTCA GGCCGCGATG GACGGCTTCG AGGTCGTGAC CCTTGATGAT GCCGCCTCCA CCGCCGATAT CGTCGTGACG ACCACCGGCA ACAAGGACGT CATCACGATC GACCATATGC GCAAGATGAA AGACATGTGC ATCGTCGGCA ATATCGGCCA CTTCGACAAT GAAATTCAGG TCGCAGCCCT GCGCAACCTG AAATGGACCA ATGTGAAGCC GCAGGTCGAT CTGATCGAGT TCCCCGACGG CAAGCGCCTC ATCCTTCTTT CGGAAGGCCG CCTGCTCAAT CTGGGCAATG CGACGGGCCA TCCAAGCTTT GTCATGTCGG CTTCCTTCAC CAATCAGGTG TTGGGCCAGA TCGAACTTTT CACCCGCACC GATGCCTATA AGAACGAAGT CTATGTGCTG CCGAAGCACC TTGATGAAAA GGTCGCTCGT CTTCATCTCG ACAAGCTTGG CGCAAAGCTG ACGGTACTTT CCGAGGAACA GGCTGCCTAT ATCGGCGTCA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A
Details for BrabA.00032.a.B1.PW29481
| PURIFICATION DATe: | 4/19/2010 |
| CONCENTRATION: | 29.93mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 80 |
| PERCENT COVERAGE: | 84 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT ASQDFVVKDI SLADWGRKEL DIAETEMPGL MAAREEFGKS QPLKGARISG SLHMTIQTAV LIETLKVLGA EVRWASCNIF STQDHAAAAI AATGTPVFAV KGETLEEYWT YTDQIFQWPD GEPSNMILDD GGDATMYILI GARAEAGEDV LSNPQSEEEE VLFAQIKKRM AATPGFFTKQ RAAIKGVTEE TTTGVNRLYQ LQKKGLLPFP AINVNDSVTK SKFDNKYGCK ESLVDGIRRG TDVMMAGKVA VVCGYGDVGK GSAQSLAGAG ARVKVTEVDP ICALXAAMDG FXVVTLDDAA SXADIVVTTT GXRTSSXSTI CXXXXCASXX XXXXXXXXXA XXXXXXXXXX XRXDXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXPXXXX
Validated NT Sequence
tnnnnnnttt tnnttaactt taagaaggag atataccatg gctcaccacc accaccacca tatgaccgca agccaagatt ttgttgtcaa ggatattagc ctggccgatt ggggccgcaa ggaactcgac atcgcagaaa ccgaaatgcc gggcctgatg gctgcccgcg aggaattcgg caagtcgcag ccgctgaagg gcgcgcgcat ttccggctcg ctgcacatga ccattcaaac tgccgttctc attgagacgc tgaaggttct gggtgccgaa gtgcgctggg cttcctgcaa cattttctcc acgcaggatc acgcggcggc ggcgattgcg gcaaccggca ccccggtttt cgccgtcaag ggcgagacgc ttgaagaata ctggacctat accgaccaga tcttccagtg gccggatggc gagccgtcca acatgatcct cgatgatggc ggcgacgcca ccatgtatat tctcatcggc gcgcgcgccg aggctggcga ggacgtcctg tccaatccgc agagcgagga agaagaagtt cttttcgcgc agatcaagaa gcgcatggcc gcaacgccgg gcttcttcac gaagcagcgc gccgcaatca agggcgtgac ggaagaaacg accaccggcg tcaaccgcct ctatcagttg cagaagaagg gtctcctgcc cttccccgcc atcaacgtca atgacagtgt gaccaagtcg aagttcgaca acaagtatgg ctgcaaggaa tcgctggtgg atggcattcg tcgcggcacc gacgtcatga tggccggcaa ggttgccgtc gtctgcggct atggtgatgt cggcaaaggc tctgcccagt cgctcgccgg tgccggcgct cgcgtcaagg tcacggaagt cgatccgatc tgcgcgcttc nggccgcgat ggacggcttc naggtcgtga ccctngatga tgccgcctcc nacgccgata tcgtcgtgac naccaccggn ncaaggacgt catcacnatc gaccatatgc nnanagaana natgtgcatc gncngnnnat cnncnnntcg anangnantn ngncgcannc ntnnncnnnn aanngannng nnnnnnnngn cgatntgatc nnntccnang nnagnnncnn nnnntnntnn nnnnnncnnn nnnnnnnnnt tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nannttnnnc cnnnnnnnnn nnnccnt
Expressed Protein Sequence
MAHHHHHHMT ASQDFVVKDI SLADWGRKEL DIAETEMPGL MAAREEFGKS QPLKGARISG SLHMTIQTAV LIETLKVLGA EVRWASCNIF STQDHAAAAI AATGTPVFAV KGETLEEYWT YTDQIFQWPD GEPSNMILDD GGDATMYILI GARAEAGEDV LSNPQSEEEE VLFAQIKKRM AATPGFFTKQ RAAIKGVTEE TTTGVNRLYQ LQKKGLLPFP AINVNDSVTK SKFDNKYGCK ESLVDGIRRG TDVMMAGKVA VVCGYGDVGK GSAQSLAGAG ARVKVTEVDP ICALQAAMDG FEVVTLDDAA STADIVVTTT GNKDVITIDH MRKMKDMCIV GNIGHFDNEI QVAALRNLKW TNVKPQVDLI EFPDGKRLIL LSEGRLLNLG NATGHPSFVM SASFTNQVLG QIELFTRTDA YKNEVYVLPK HLDEKVARLH LDKLGAKLTV LSEEQAAYIG VTPQGPFKSE HYRY
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACCG CAAGCCAAGA TTTTGTTGTC AAGGATATTA GCCTGGCCGA TTGGGGCCGC AAGGAACTCG ACATCGCAGA AACCGAAATG CCGGGCCTGA TGGCTGCCCG CGAGGAATTC GGCAAGTCGC AGCCGCTGAA GGGCGCGCGC ATTTCCGGCT CGCTGCACAT GACCATTCAA ACTGCCGTTC TCATTGAGAC GCTGAAGGTT CTGGGTGCCG AAGTGCGCTG GGCTTCCTGC AACATTTTCT CCACGCAGGA TCACGCGGCG GCGGCGATTG CGGCAACCGG CACCCCGGTT TTCGCCGTCA AGGGCGAGAC GCTTGAAGAA TACTGGACCT ATACCGACCA GATCTTCCAG TGGCCGGATG GCGAGCCGTC CAACATGATC CTCGATGATG GCGGCGACGC CACCATGTAT ATTCTCATCG GCGCGCGCGC CGAGGCTGGC GAGGACGTCC TGTCCAATCC GCAGAGCGAG GAAGAAGAAG TTCTTTTCGC GCAGATCAAG AAGCGCATGG CCGCAACGCC GGGCTTCTTC ACGAAGCAGC GCGCCGCAAT CAAGGGCGTG ACGGAAGAAA CGACCACCGG CGTCAACCGC CTCTATCAGT TGCAGAAGAA GGGTCTCCTG CCCTTCCCCG CCATCAACGT CAATGACAGT GTGACCAAGT CGAAGTTCGA CAACAAGTAT GGCTGCAAGG AATCGCTGGT GGATGGCATT CGTCGCGGCA CCGACGTCAT GATGGCCGGC AAGGTTGCCG TCGTCTGCGG CTATGGTGAT GTCGGCAAAG GCTCTGCCCA GTCGCTCGCC GGTGCCGGCG CTCGCGTCAA GGTCACGGAA GTCGATCCGA TCTGCGCGCT TCAGGCCGCG ATGGACGGCT TCGAGGTCGT GACCCTTGAT GATGCCGCCT CCACCGCCGA TATCGTCGTG ACGACCACCG GCAACAAGGA CGTCATCACG ATCGACCATA TGCGCAAGAT GAAAGACATG TGCATCGTCG GCAATATCGG CCACTTCGAC AATGAAATTC AGGTCGCAGC CCTGCGCAAC CTGAAATGGA CCAATGTGAA GCCGCAGGTC GATCTGATCG AGTTCCCCGA CGGCAAGCGC CTCATCCTTC TTTCGGAAGG CCGCCTGCTC AATCTGGGCA ATGCGACGGG CCATCCAAGC TTTGTCATGT CGGCTTCCTT CACCAATCAG GTGTTGGGCC AGATCGAACT TTTCACCCGC ACCGATGCCT ATAAGAACGA AGTCTATGTG CTGCCGAAGC ACCTTGATGA AAAGGTCGCT CGTCTTCATC TCGACAAGCT TGGCGCAAAG CTGACGGTAC TTTCCGAGGA ACAGGCTGCC TATATCGGCG TCACTCCACA GGGTCCGTTC AAGTCGGAAC ACTACAGGTA TTAATGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT
Details for BrabA.00032.a.B1.PW25512
| PURIFICATION DATe: | 4/19/2010 |
| CONCENTRATION: | 27mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 12 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 80 |
| PERCENT COVERAGE: | 84 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT ASQDFVVKDI SLADWGRKEL DIAETEMPGL MAAREEFGKS QPLKGARISG SLHMTIQTAV LIETLKVLGA EVRWASCNIF STQDHAAAAI AATGTPVFAV KGETLEEYWT YTDQIFQWPD GEPSNMILDD GGDATMYILI GARAEAGEDV LSNPQSEEEE VLFAQIKKRM AATPGFFTKQ RAAIKGVTEE TTTGVNRLYQ LQKKGLLPFP AINVNDSVTK SKFDNKYGCK ESLVDGIRRG TDVMMAGKVA VVCGYGDVGK GSAQSLAGAG ARVKVTEVDP ICALXAAMDG FXVVTLDDAA SXADIVVTTT GXRTSSXSTI CXXXXCASXX XXXXXXXXXA XXXXXXXXXX XRXDXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXPXXXX
Validated NT Sequence
tnnnnnnttt tnnttaactt taagaaggag atataccatg gctcaccacc accaccacca tatgaccgca agccaagatt ttgttgtcaa ggatattagc ctggccgatt ggggccgcaa ggaactcgac atcgcagaaa ccgaaatgcc gggcctgatg gctgcccgcg aggaattcgg caagtcgcag ccgctgaagg gcgcgcgcat ttccggctcg ctgcacatga ccattcaaac tgccgttctc attgagacgc tgaaggttct gggtgccgaa gtgcgctggg cttcctgcaa cattttctcc acgcaggatc acgcggcggc ggcgattgcg gcaaccggca ccccggtttt cgccgtcaag ggcgagacgc ttgaagaata ctggacctat accgaccaga tcttccagtg gccggatggc gagccgtcca acatgatcct cgatgatggc ggcgacgcca ccatgtatat tctcatcggc gcgcgcgccg aggctggcga ggacgtcctg tccaatccgc agagcgagga agaagaagtt cttttcgcgc agatcaagaa gcgcatggcc gcaacgccgg gcttcttcac gaagcagcgc gccgcaatca agggcgtgac ggaagaaacg accaccggcg tcaaccgcct ctatcagttg cagaagaagg gtctcctgcc cttccccgcc atcaacgtca atgacagtgt gaccaagtcg aagttcgaca acaagtatgg ctgcaaggaa tcgctggtgg atggcattcg tcgcggcacc gacgtcatga tggccggcaa ggttgccgtc gtctgcggct atggtgatgt cggcaaaggc tctgcccagt cgctcgccgg tgccggcgct cgcgtcaagg tcacggaagt cgatccgatc tgcgcgcttc nggccgcgat ggacggcttc naggtcgtga ccctngatga tgccgcctcc nacgccgata tcgtcgtgac naccaccggn ncaaggacgt catcacnatc gaccatatgc nnanagaana natgtgcatc gncngnnnat cnncnnntcg anangnantn ngncgcannc ntnnncnnnn aanngannng nnnnnnnngn cgatntgatc nnntccnang nnagnnncnn nnnntnntnn nnnnnncnnn nnnnnnnnnt tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nannttnnnc cnnnnnnnnn nnnccnt
Expressed Protein Sequence
MAHHHHHHMT ASQDFVVKDI SLADWGRKEL DIAETEMPGL MAAREEFGKS QPLKGARISG SLHMTIQTAV LIETLKVLGA EVRWASCNIF STQDHAAAAI AATGTPVFAV KGETLEEYWT YTDQIFQWPD GEPSNMILDD GGDATMYILI GARAEAGEDV LSNPQSEEEE VLFAQIKKRM AATPGFFTKQ RAAIKGVTEE TTTGVNRLYQ LQKKGLLPFP AINVNDSVTK SKFDNKYGCK ESLVDGIRRG TDVMMAGKVA VVCGYGDVGK GSAQSLAGAG ARVKVTEVDP ICALQAAMDG FEVVTLDDAA STADIVVTTT GNKDVITIDH MRKMKDMCIV GNIGHFDNEI QVAALRNLKW TNVKPQVDLI EFPDGKRLIL LSEGRLLNLG NATGHPSFVM SASFTNQVLG QIELFTRTDA YKNEVYVLPK HLDEKVARLH LDKLGAKLTV LSEEQAAYIG VTPQGPFKSE HYRY
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACCG CAAGCCAAGA TTTTGTTGTC AAGGATATTA GCCTGGCCGA TTGGGGCCGC AAGGAACTCG ACATCGCAGA AACCGAAATG CCGGGCCTGA TGGCTGCCCG CGAGGAATTC GGCAAGTCGC AGCCGCTGAA GGGCGCGCGC ATTTCCGGCT CGCTGCACAT GACCATTCAA ACTGCCGTTC TCATTGAGAC GCTGAAGGTT CTGGGTGCCG AAGTGCGCTG GGCTTCCTGC AACATTTTCT CCACGCAGGA TCACGCGGCG GCGGCGATTG CGGCAACCGG CACCCCGGTT TTCGCCGTCA AGGGCGAGAC GCTTGAAGAA TACTGGACCT ATACCGACCA GATCTTCCAG TGGCCGGATG GCGAGCCGTC CAACATGATC CTCGATGATG GCGGCGACGC CACCATGTAT ATTCTCATCG GCGCGCGCGC CGAGGCTGGC GAGGACGTCC TGTCCAATCC GCAGAGCGAG GAAGAAGAAG TTCTTTTCGC GCAGATCAAG AAGCGCATGG CCGCAACGCC GGGCTTCTTC ACGAAGCAGC GCGCCGCAAT CAAGGGCGTG ACGGAAGAAA CGACCACCGG CGTCAACCGC CTCTATCAGT TGCAGAAGAA GGGTCTCCTG CCCTTCCCCG CCATCAACGT CAATGACAGT GTGACCAAGT CGAAGTTCGA CAACAAGTAT GGCTGCAAGG AATCGCTGGT GGATGGCATT CGTCGCGGCA CCGACGTCAT GATGGCCGGC AAGGTTGCCG TCGTCTGCGG CTATGGTGAT GTCGGCAAAG GCTCTGCCCA GTCGCTCGCC GGTGCCGGCG CTCGCGTCAA GGTCACGGAA GTCGATCCGA TCTGCGCGCT TCAGGCCGCG ATGGACGGCT TCGAGGTCGT GACCCTTGAT GATGCCGCCT CCACCGCCGA TATCGTCGTG ACGACCACCG GCAACAAGGA CGTCATCACG ATCGACCATA TGCGCAAGAT GAAAGACATG TGCATCGTCG GCAATATCGG CCACTTCGAC AATGAAATTC AGGTCGCAGC CCTGCGCAAC CTGAAATGGA CCAATGTGAA GCCGCAGGTC GATCTGATCG AGTTCCCCGA CGGCAAGCGC CTCATCCTTC TTTCGGAAGG CCGCCTGCTC AATCTGGGCA ATGCGACGGG CCATCCAAGC TTTGTCATGT CGGCTTCCTT CACCAATCAG GTGTTGGGCC AGATCGAACT TTTCACCCGC ACCGATGCCT ATAAGAACGA AGTCTATGTG CTGCCGAAGC ACCTTGATGA AAAGGTCGCT CGTCTTCATC TCGACAAGCT TGGCGCAAAG CTGACGGTAC TTTCCGAGGA ACAGGCTGCC TATATCGGCG TCACTCCACA GGGTCCGTTC AAGTCGGAAC ACTACAGGTA TTAATGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT