BrabA.00032.a
S-adenosyl-L-homocysteine hydrolase:ATP/GTP-binding site motif A (P-loop)
CENTER ID:
BrabA.00032.a
ORGANISM:
Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:
Brucellosis
CURRENT STATUS:
in PDB
COMMUNITY REQUEST:
False
NIH RISK GROUP:
3
SELECT AGENT:
True
NIH PRIORITY
pathogens category:
IIIB
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO
AA
START
AA
STOP
ORDER
MATERIAL
BrabA.00032.a.A2.GE28020
Created from Decode construct for feature BrabA.00032.a
info
1
453
BrabA.00032.a.A2.GE28020
BrabA.00032.a.A3.GE28021
Created from Decode construct for feature BrabA.00032.a
info
4
466
BrabA.00032.a.A3.GE28021
BrabA.00032.a.A4.GE28022
Created from Decode construct for feature BrabA.00032.a
info
4
453
BrabA.00032.a.A4.GE28022
BrabA.00032.a.A5.GE28023
Created from Decode construct for feature BrabA.00032.a
info
8
466
BrabA.00032.a.A5.GE28023
BrabA.00032.a.A6.GE28024
Created from Decode construct for feature BrabA.00032.a
info
8
453
BrabA.00032.a.A6.GE28024
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis . Get sequence information
using the button in the "info" column.
Proteins
CENTER REFERENCE ID
DOMAIN/REGION DESCRIPTION
INFO
AA START
AA STOP
ORDER MATERIAL
BrabA.00032.a.A5.PS00514
Created from Decode construct for feature BrabA.00032.a
info
8
466
PS00514
BrabA.00032.a.A2.PS00516
Created from Decode construct for feature BrabA.00032.a
info
1
453
PS00516
BrabA.00032.a.A6.PS00517
Created from Decode construct for feature BrabA.00032.a
info
8
453
PS00517
BrabA.00032.a.A4.PS00520
Created from Decode construct for feature BrabA.00032.a
info
4
453
PS00520
BrabA.00032.a.A3.PS00530
Created from Decode construct for feature BrabA.00032.a
info
4
466
PS00530
BrabA.00032.a.B1.PW25512
Full length( BrabA.00032.a )
info
1
466
PW25512
BrabA.00032.a.B1.PW29481
Full length( BrabA.00032.a )
info
1
466
PW29481
Structures
3N58
DEPOSITED: 5/24/2010
DETERMINATION: XRay
CLONE: BrabA.00032.a.A5.GE28023
PROTEIN: BrabA.00032.a.A5.PS00514
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTASQDFVVK DISLADWGRK ELDIAETEMP GLMAAREEFG KSQPLKGARI SGSLHMTIQT AVLIETLKVL GAEVRWASCN IFSTQDHAAA AIAATGTPVF AVKGETLEEY WTYTDQIFQW PDGEPSNMIL DDGGDATMYI LIGARAEAGE DVLSNPQSEE EEVLFAQIKK RMAATPGFFT KQRAAIKGVT EETTTGVNRL YQLQKKGLLP FPAINVNDSV TKSKFDNKYG CKESLVDGIR RGTDVMMAGK VAVVCGYGDV GKGSAQSLAG AGARVKVTEV DPICALQAAM DGFEVVTLDD AASTADIVVT TTGNKDVITI DHMRKMKDMC IVGNIGHFDN EIQVAALRNL KWTNVKPQVD LIEFPDGKRL ILLSEGRLLN LGNATGHPSF VMSASFTNQV LGQIELFTRT DAYKNEVYVL PKHLDEKVAR LHLDKLGAKL TVLSEEQAAY IGVTPQGPFK SEHYRY
NT Sequence
ATGACCGCAA GCCAAGATTT TGTTGTCAAG GATATTAGCC TGGCCGATTG GGGCCGCAAG GAACTCGACA TCGCAGAAAC CGAAATGCCG GGCCTGATGG CTGCCCGCGA GGAATTCGGC AAGTCGCAGC CGCTGAAGGG CGCGCGCATT TCCGGCTCGC TGCACATGAC CATTCAAACT GCCGTTCTCA TTGAGACGCT GAAGGTTCTG GGTGCCGAAG TGCGCTGGGC TTCCTGCAAC ATTTTCTCCA CGCAGGATCA CGCGGCGGCG GCGATTGCGG CAACCGGCAC CCCGGTTTTC GCCGTCAAGG GCGAGACGCT TGAAGAATAC TGGACCTATA CCGACCAGAT CTTCCAGTGG CCGGATGGCG AGCCGTCCAA CATGATCCTC GATGATGGCG GCGACGCCAC CATGTATATT CTCATCGGCG CGCGCGCCGA GGCTGGCGAG GACGTCCTGT CCAATCCGCA GAGCGAGGAA GAAGAAGTTC TTTTCGCGCA GATCAAGAAG CGCATGGCCG CAACGCCGGG CTTCTTCACG AAGCAGCGCG CCGCAATCAA GGGCGTGACG GAAGAAACGA CCACCGGCGT CAACCGCCTC TATCAGTTGC AGAAGAAGGG TCTCCTGCCC TTCCCCGCCA TCAACGTCAA TGACAGTGTG ACCAAGTCGA AGTTCGACAA CAAGTATGGC TGCAAGGAAT CGCTGGTGGA TGGCATTCGT CGCGGCACCG ACGTCATGAT GGCCGGCAAG GTTGCCGTCG TCTGCGGCTA TGGTGATGTC GGCAAAGGCT CTGCCCAGTC GCTCGCCGGT GCCGGCGCTC GCGTCAAGGT CACGGAAGTC GATCCGATCT GCGCGCTTCA GGCCGCGATG GACGGCTTCG AGGTCGTGAC CCTTGATGAT GCCGCCTCCA CCGCCGATAT CGTCGTGACG ACCACCGGCA ACAAGGACGT CATCACGATC GACCATATGC GCAAGATGAA AGACATGTGC ATCGTCGGCA ATATCGGCCA CTTCGACAAT GAAATTCAGG TCGCAGCCCT GCGCAACCTG AAATGGACCA ATGTGAAGCC GCAGGTCGAT CTGATCGAGT TCCCCGACGG CAAGCGCCTC ATCCTTCTTT CGGAAGGCCG CCTGCTCAAT CTGGGCAATG CGACGGGCCA TCCAAGCTTT GTCATGTCGG CTTCCTTCAC CAATCAGGTG TTGGGCCAGA TCGAACTTTT CACCCGCACC GATGCCTATA AGAACGAAGT CTATGTGCTG CCGAAGCACC TTGATGAAAA GGTCGCTCGT CTTCATCTCG ACAAGCTTGG CGCAAAGCTG ACGGTACTTT CCGAGGAACA GGCTGCCTAT ATCGGCGTCA CTCCACAGGG TCCGTTCAAG TCGGAACACT ACAGGTAT
Details for BrabA.00032.a.A2.GE28020
HARVESTED ON:
12/7/2009
SEQUENCED ON:
12/23/2009
EXPECTED MW:
51kDa
OBSERVED MW:
51kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Good (10-50)
TOTAL EXPRESSION LEVEL:
Moderate Expression
SOLUBLE EXPRESSION LEVEL
High Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass
PERCENT IDENTITY:
96
PERCENT COVERAGE:
59
Details for BrabA.00032.a.A3.GE28021
HARVESTED ON:
12/7/2009
SEQUENCED ON:
12/23/2009
EXPECTED MW:
52kDa
OBSERVED MW:
52kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Good (10-50)
TOTAL EXPRESSION LEVEL:
Low Expression
SOLUBLE EXPRESSION LEVEL
Low Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for BrabA.00032.a.A4.GE28022
HARVESTED ON:
12/7/2009
SEQUENCED ON:
12/23/2009
EXPECTED MW:
51kDa
OBSERVED MW:
52kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Good (10-50)
TOTAL EXPRESSION LEVEL:
Low Expression
SOLUBLE EXPRESSION LEVEL
Low Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass
PERCENT IDENTITY:
92
PERCENT COVERAGE:
48
Details for BrabA.00032.a.A5.GE28023
HARVESTED ON:
12/7/2009
SEQUENCED ON:
12/23/2009
EXPECTED MW:
52kDa
OBSERVED MW:
52kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Good (10-50)
TOTAL EXPRESSION LEVEL:
Moderate Expression
SOLUBLE EXPRESSION LEVEL
High Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for BrabA.00032.a.A6.GE28024
HARVESTED ON:
12/7/2009
SEQUENCED ON:
12/23/2009
EXPECTED MW:
50kDa
OBSERVED MW:
51kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Good (10-50)
TOTAL EXPRESSION LEVEL:
Moderate Expression
SOLUBLE EXPRESSION LEVEL
High Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass with incomplete coverage
PERCENT IDENTITY:
100
PERCENT COVERAGE:
95
Details for BrabA.00032.a.A5.PS00514
PURIFICATION DATe:
3/12/2010
CONCENTRATION:
79.84mg/ml
OBSERVED MW:
data unavailable
EXPRESSION LEVEL:
High Expression
PROTEIN PURIFICATION BUFFER:
20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
4
VIAL VOLUME:
200µl
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for BrabA.00032.a.A2.PS00516
PURIFICATION DATe:
3/12/2010
CONCENTRATION:
99.05mg/ml
OBSERVED MW:
data unavailable
EXPRESSION LEVEL:
High Expression
PROTEIN PURIFICATION BUFFER:
20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
10
VIAL VOLUME:
100µl
PERCENT IDENTITY:
96
PERCENT COVERAGE:
59
Details for BrabA.00032.a.A6.PS00517
PURIFICATION DATe:
3/12/2010
CONCENTRATION:
37.41mg/ml
OBSERVED MW:
data unavailable
EXPRESSION LEVEL:
High Expression
PROTEIN PURIFICATION BUFFER:
20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
7
VIAL VOLUME:
200µl
PERCENT IDENTITY:
100
PERCENT COVERAGE:
95
Details for BrabA.00032.a.A4.PS00520
PURIFICATION DATe:
3/11/2010
CONCENTRATION:
22.35mg/ml
OBSERVED MW:
data unavailable
EXPRESSION LEVEL:
Moderate Expression
PROTEIN PURIFICATION BUFFER:
20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
7
VIAL VOLUME:
200µl
PERCENT IDENTITY:
92
PERCENT COVERAGE:
48
Details for BrabA.00032.a.A3.PS00530
PURIFICATION DATe:
3/11/2010
CONCENTRATION:
18.1mg/ml
OBSERVED MW:
data unavailable
EXPRESSION LEVEL:
High Expression
PROTEIN PURIFICATION BUFFER:
20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
9
VIAL VOLUME:
100µl
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for BrabA.00032.a.B1.PW25512
PURIFICATION DATe:
4/19/2010
CONCENTRATION:
27mg/ml
OBSERVED MW:
data unavailable
EXPRESSION LEVEL:
Moderate Expression
PROTEIN PURIFICATION BUFFER:
25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
12
VIAL VOLUME:
200µl
PERCENT IDENTITY:
80
PERCENT COVERAGE:
84
Details for BrabA.00032.a.B1.PW29481
PURIFICATION DATe:
4/19/2010
CONCENTRATION:
29.93mg/ml
OBSERVED MW:
data unavailable
EXPRESSION LEVEL:
Moderate Expression
PROTEIN PURIFICATION BUFFER:
25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
4
VIAL VOLUME:
100µl
PERCENT IDENTITY:
80
PERCENT COVERAGE:
84