BrabA.00030.a

CbxX/CfqX superfamily:ATP/GTP-binding site motif A (P-loop):Bacterial chromosomal replication initiator protein, DnaA:AAA ATP...

CENTER ID: BrabA.00030.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BrabA.00030.a.A1.GE25218 Full length( BrabA.00030.a ) 1 424
BrabA.00030.a.A2.GE28015 Created from Decode construct for feature BrabA.00030.a 1 404
BrabA.00030.a.A3.GE28016 Created from Decode construct for feature BrabA.00030.a 1 411
BrabA.00030.a.A4.GE28017 Created from Decode construct for feature BrabA.00030.a 64 424
BrabA.00030.a.A6.GE28019 Created from Decode construct for feature BrabA.00030.a 64 411
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BrabA.00030.a.A3.PS00504 Created from Decode construct for feature BrabA.00030.a 1 411
BrabA.00030.a.A6.PS00505 Created from Decode construct for feature BrabA.00030.a 64 411
BrabA.00030.a.A2.PS00506 Created from Decode construct for feature BrabA.00030.a 1 404
BrabA.00030.a.A4.PS00508 Created from Decode construct for feature BrabA.00030.a 64 424
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_128011
PATRIC ID: fig|359391.4.peg.1180
RefSeq: YP_414524
UniProt: Q2YPX2
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSKVSNGGGD SKNTLYCSFC GKSQHEVRKL IAGPTVFICD ECVELCMDII REENKSSMVK SREGVPTPQE IMAVLDDYVI GQKDAKRVLS VAVHNHYKRL AHQSKNSDIE LAKSNILLVG PTGCGKTYLA QTLARIIDVP FIMADATTLT EAGYVGEDVE NIILKLLQAA DYNVERAQRG IVYIDEVDKI SRKSDNPSIT RDVSGEGVQQ ALLKIMEGTV ASVPPQGGRK HPQQEFLQVD TTNILFICGG AFAGLDRIIS ARGEKTSIGF GATVKSVDER RIGEVFKELE PEDLLKFGLI PEFVGRLPVI ATLEDLDVDA LVQILTEPKN ALVKQYQRLF DMENVELVFH DDALRAIANK AVERKTGARG LRSIMEKILL DTMFELPTLE GVREVVISGD VVDGSARPLY IYAERQDEKG NVSA
NT Sequence
ATGAGCAAAG TCAGCAACGG CGGCGGCGAT TCCAAGAATA CGCTTTATTG CTCGTTCTGT GGCAAAAGCC AGCATGAAGT GCGCAAGCTG ATTGCAGGCC CGACCGTTTT CATCTGCGAC GAATGCGTCG AACTCTGCAT GGACATCATC CGTGAGGAAA ACAAATCCTC CATGGTGAAG TCGCGTGAGG GAGTACCGAC GCCGCAGGAA ATCATGGCCG TCCTTGACGA TTACGTCATC GGCCAGAAGG ATGCCAAGCG CGTTCTGTCG GTTGCGGTTC ACAATCATTA CAAGCGTCTG GCGCATCAGT CCAAGAACAG CGATATCGAA CTGGCGAAGT CGAACATTCT GCTCGTCGGC CCGACGGGCT GCGGCAAGAC CTATCTTGCC CAGACGCTTG CCCGCATCAT CGATGTGCCG TTCATCATGG CGGATGCGAC GACGCTGACG GAAGCCGGCT ATGTCGGCGA AGATGTGGAG AACATCATCC TGAAGCTGCT TCAGGCCGCC GATTATAATG TGGAGCGCGC GCAGCGCGGC ATCGTCTATA TTGATGAAGT GGACAAGATC AGCCGCAAGT CGGACAATCC ATCCATTACC CGTGACGTAT CGGGCGAGGG TGTGCAGCAG GCCCTGCTGA AGATCATGGA AGGCACTGTC GCTTCCGTGC CGCCGCAGGG TGGGCGCAAG CATCCGCAGC AGGAATTCCT GCAGGTGGAT ACGACAAACA TCCTGTTTAT CTGCGGTGGT GCTTTTGCGG GTCTCGACCG TATCATTTCC GCTCGCGGCG AGAAGACCTC CATCGGCTTT GGCGCTACGG TCAAGTCCGT CGATGAGCGT CGTATCGGTG AAGTCTTCAA GGAATTGGAA CCGGAAGACC TGTTGAAATT CGGCCTGATT CCGGAATTTG TCGGTCGTCT GCCTGTCATC GCTACGCTCG AAGACCTCGA CGTCGATGCT CTGGTCCAGA TTCTGACCGA GCCGAAGAAT GCGCTCGTGA AGCAGTATCA GCGCCTGTTC GACATGGAAA ATGTCGAACT GGTGTTCCAT GACGATGCGT TGCGCGCGAT TGCCAACAAG GCTGTCGAAC GCAAGACGGG TGCACGCGGC CTGCGTTCGA TCATGGAAAA GATTCTGCTC GATACCATGT TTGAACTGCC GACGCTGGAA GGCGTTCGCG AAGTTGTGAT TTCGGGCGAT GTGGTGGATG GAAGCGCCCG CCCGCTATAC ATCTACGCCG AGCGGCAGGA CGAAAAGGGC AACGTTTCAG CC
Details for BrabA.00030.a.A1.GE25218
HARVESTED ON: 5/6/2008
SEQUENCED ON: 6/16/2009
EXPECTED MW: 48kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSKVSNGGG DSKNTLYCSF CGKSQHEVRK LIAGPTVFIC DECVELCMDI IREENKSSMV KSREGVPTPQ EIMAVLDDYV IGQKDAKRVL SVAVHNHYKR LAHQSKNSDI ELAKSNILLV GPTGCGKTYL AQTLARIIDV PFIMADATTL TEAGYVGEDV ENIILKLLQA ADYNVERAQR GIVYIDEVDK ISRKSDNPSI TRDVSGEGVQ QALLKIMEGT VASVPPQGGR KHPQQEFLQV DTTNILFICG GAFAGLDRII SARGEKTSIG FGATVKSVDE RRIGEVFKEL EPEDLLKFGL IPEFVGRLPV IATLEDLDVD ALVQILTEPK NALVKQYQRL FDMENVELVF HDDALRAIAN KAVERKTGAR GLRSIMEKIL LDTMFELPTL EGVREVVISG DVVDGSARPL YIYAERQDEK GNVSA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcaaagtc agcaacggcg gcggcgattc caagaatacg ctttattgct cgttctgtgg caaaagccag catgaagtgc gcaagctgat tgcaggcccg accgttttca tctgcgacga atgcgtcgaa ctctgcatgg acatcatccg tgaggaaaac aaatcctcca tggtgaagtc gcgtgaggga gtaccgacgc cgcaggaaat catggccgtc cttgacgatt acgtcatcgg ccagaaggat gccaagcgcg ttctgtcggt tgcggttcac aatcattaca agcgtctggc gcatcagtcc aagaacagcg atatcgaact ggcgaagtcg aacattctgc tcgtcggccc gacgggctgc ggcaagacct atcttgccca gacgcttgcc cgcatcatcg atgtgccgtt catcatggcg gatgcgacga cgctgacgga agccggctat gtcggcgaag atgtggagaa catcatcctg aagctgcttc aggccgccga ttataatgtg gagcgcgcgc agcgcggcat cgtctatatt gatgaagtgg acaagatcag ccgcaagtcg gacaatccat ccattacccg tgacgtatcg ggcgagggtg tgcagcaggc cctgctgaag atcatggaag gcactgtcgc ttccgtgccg ccgcagggtg ggcgcaagca tccgcagcag gaattcctgc aggtggatac gacaaacatc ctgtttatct gcggtggtgc ttttgcgggt ctcgaccgta tcatttccgc tcgcggcgag aagacctcca tcggctttgg cgctacggtc aagtccgtcg atgagcgtcg tatcggtgaa gtcttcaagg aattggaacc ggaagacctg ttgaaattcg gcctgattcc ggaatttgtc ggtcgtctgc ctgtcatcgc tacgctcgaa gacctcgacg tcgatgctct ggtccagatt ctgaccgagc cgaagaatgc gctcgtgaag cagtatcagc gcctgttcga catggaaaat gtcgaactgg tgttccatga cgatgcgttg cgcgcgattg ccaacaaggc tgtcgaacgc aagacgggtg cacgcggcct gcgttcgatc atggaaaaga ttctgctcga taccatgttt gaactgccga cgctggaagg cgttcgcgaa gttgtgattt cgggcgatgt ggtggatgga agcgcccgcc cgctatacat ctacgccgag cggcaggacg aaaagggcaa cgtttcagcc tgaaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for BrabA.00030.a.A2.GE28015
HARVESTED ON: 12/7/2009
SEQUENCED ON: 12/23/2009
EXPECTED MW: 46kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSKVSNGGG DSKNTLYCSF CGKSQHEVRK LIAGPTVFIC DECVELCMDI IREENKSSMV KSREGVPTPQ EIMAVLDDYV IGQKDAKRVL SVAVHNHYKR LAHQSKNSDI ELAKSNILLV GPTGCGKTYL AQTLARIIDV PFIMADATTL TEAGYVGEDV ENIILKLLQA ADYNVERAQR GIVYIDEVXK ISRKSDNPSI TRDVSGEGVQ QALLKIMEGT VASVPPQGGR KHPQQEFLQV DTTNILFICG GAFAGLDRII SARGEKTSIG FGATVKSVDE RRIGEVFKEL EPEDLLKFGL IPEFVGRLPV IATLEDLDVD ALVQILTEPK NALVKQYQRL FDMENVELVF HDDALRAIAN KAVERKTGAR GLRSIMEKIL LDTMFELPTL EGVREVVISG DVVDG
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSKVSNGGG DSKNTLYCSF CGKSQHEVRK LIAGPTVFIC DECVELCMDI IREENKSSMV KSREGVPTPQ EIMAVLDDYV IGQKDAKRVL SVAVHNHYKR LAHQSKNSDI ELAKSNILLV GPTGCGKTYL AQTLARIIDV PFIMADATTL TEAGYVGEDV ENIILKLLQA ADYNVERAQR GIVYIDEVDK ISRKSDNPSI TRDVSGEGVQ QALLKIMEGT VASVPPQGGR KHPQQEFLQV DTTNILFICG GAFAGLDRII SARGEKTSIG FGATVKSVDE RRIGEVFKEL EPEDLLKFGL IPEFVGRLPV IATLEDLDVD ALVQILTEPK NALVKQYQRL FDMENVELVF HDDALRAIAN KAVERKTGAR GLRSIMEKIL LDTMFELPTL EGVREVVISG DVVDG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcaaagtc agcaacggcg gcggcgattc caagaatacg ctttattgct cgttctgtgg caaaagccag catgaagtgc gcaagctgat tgcaggcccg accgttttca tctgcgacga atgcgtcgaa ctctgcatgg acatcatccg tgaggaaaac aaatcctcca tggtgaagtc gcgtgaggga gtaccgacgc cgcaggaaat catggccgtc cttgacgatt acgtcatcgg ccagaaggat gccaagcgcg ttctgtcggt tgcggttcac aatcattaca agcgtctggc gcatcagtcc aagaacagcg atatcgaact ggcgaagtcg aacattctgc tcgtcggccc gacgggctgc ggcaagacct atcttgccca gacgcttgcc cgcatcatcg atgtgccgtt catcatggcg gatgcgacga cgctgacgga agccggctat gtcggcgaag atgtggagaa catcatcctg aagctgcttc aggccgccga ttataatgtg gagcgcgcgc agcgcggcat cgtctatatt gatgaagtgg acaagatcag ccgcaagtcg gacaatccat ccattacccg tgacgtatcg ggcgagggtg tgcagcaggc cctgctgaag atcatggaag gcactgtcgc ttccgtgccg ccgcagggtg ggcgcaagca tccgcagcag gaattcctgc aggtggatac gacaaacatc ctgtttatct gcggtggtgc ttttgcgggt ctcgaccgta tcatttccgc tcgcggcgag aagacctcca tcggctttgg cgctacggtc aagtccgtcg atgagcgtcg tatcggtgaa gtcttcaagg aattggaacc ggaagacctg ttgaaattcg gcctgattcc ggaatttgtc ggtcgtctgc ctgtcatcgc tacgctcgaa gacctcgacg tcgatgctct ggtccagatt ctgaccgagc cgaagaatgc gctcgtgaag cagtatcagc gcctgttcga catggaaaat gtcgaactgg tgttccatga cgatgcgttg cgcgcgattg ccaacaaggc tgtcgaacgc aagacgggtg cacgcggcct gcgttcgatc atggaaaaga ttctgctcga taccatgttt gaactgccga cgctggaagg cgttcgcgaa gttgtgattt cgggcgatgt ggtggatgga aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for BrabA.00030.a.A3.GE28016
HARVESTED ON: 12/7/2009
SEQUENCED ON: 12/23/2009
EXPECTED MW: 47kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 67
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSKVSNGGG DSKNTLYCSF CGKSQHEVRK LIAGPTVFIC DECVELCMDI IREENKSSMV KSREGVPTPQ EIMAVLDDYV IGQKDAKRVL SVAVHNHYKR LAHQSKNSDI ELAKSNILLV GPTGCGKTYL AQTLARIIDV PFIMADATTL TEAGYVGEDV ENIILKLLQA ADYNVERAQR GIVYIDEVDK ISRKSDNPSI TRDVSGEGVQ QALLKIMEGT VASVPPXGXR KHPQXEFLQV DTTNILFIXG GAFAGLDRII SARGXKXSXG FGA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagca aagtcagcaa cggcggcggc gattccaaga atacgcttta ttgctcgttc tgtggcaaaa gccagcatga agtgcgcaag ctgattgcag gcccgaccgt tttcatctgc gacgaatgcg tcgaactctg catggacatc atccgtgagg aaaacaaatc ctccatggtg aagtcgcgtg agggagtacc gacgccgcag gaaatcatgg ccgtccttga cgattacgtc atcggccaga aggatgccaa gcgcgttctg tcggttgcgg ttcacaatca ttacaagcgt ctggcgcatc agtccaagaa cagcgatatc gaactggcga agtcgaacat tctgctcgtc ggcccgacgg gctgcggcaa gacctatctt gcccagacgc ttgcccgcat catcgatgtg ccgttcatca tggcggatgc gacgacgctg acggaagccg gctatgtcgg cgaagatgtg gagaacatca tcctgaagct gcttcaggcc gccgattata atgtggagcg cgcgcagcgc ggcatcgtct atattgatga agtggacaag atcagccgca agtcggacaa tccatccatt acccgtgacg tatcgggcga gggtgtgcag caggccctgc tgaagatcat ggaaggcact gtcgcttccg tgccgccgca nggtgngcgc aagcatccgc agcangaatt cctgcaggtg gatacnacaa acatcctgtt tatctgnggn ggtgcttttg cgggnctcga ccgtatcatt tccgctcgcg gcganaagan ctccntcggn tttggcgcta c
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSKVSNGGG DSKNTLYCSF CGKSQHEVRK LIAGPTVFIC DECVELCMDI IREENKSSMV KSREGVPTPQ EIMAVLDDYV IGQKDAKRVL SVAVHNHYKR LAHQSKNSDI ELAKSNILLV GPTGCGKTYL AQTLARIIDV PFIMADATTL TEAGYVGEDV ENIILKLLQA ADYNVERAQR GIVYIDEVDK ISRKSDNPSI TRDVSGEGVQ QALLKIMEGT VASVPPQGGR KHPQQEFLQV DTTNILFICG GAFAGLDRII SARGEKTSIG FGATVKSVDE RRIGEVFKEL EPEDLLKFGL IPEFVGRLPV IATLEDLDVD ALVQILTEPK NALVKQYQRL FDMENVELVF HDDALRAIAN KAVERKTGAR GLRSIMEKIL LDTMFELPTL EGVREVVISG DVVDGSARPL YI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcaaagtc agcaacggcg gcggcgattc caagaatacg ctttattgct cgttctgtgg caaaagccag catgaagtgc gcaagctgat tgcaggcccg accgttttca tctgcgacga atgcgtcgaa ctctgcatgg acatcatccg tgaggaaaac aaatcctcca tggtgaagtc gcgtgaggga gtaccgacgc cgcaggaaat catggccgtc cttgacgatt acgtcatcgg ccagaaggat gccaagcgcg ttctgtcggt tgcggttcac aatcattaca agcgtctggc gcatcagtcc aagaacagcg atatcgaact ggcgaagtcg aacattctgc tcgtcggccc gacgggctgc ggcaagacct atcttgccca gacgcttgcc cgcatcatcg atgtgccgtt catcatggcg gatgcgacga cgctgacgga agccggctat gtcggcgaag atgtggagaa catcatcctg aagctgcttc aggccgccga ttataatgtg gagcgcgcgc agcgcggcat cgtctatatt gatgaagtgg acaagatcag ccgcaagtcg gacaatccat ccattacccg tgacgtatcg ggcgagggtg tgcagcaggc cctgctgaag atcatggaag gcactgtcgc ttccgtgccg ccgcagggtg ggcgcaagca tccgcagcag gaattcctgc aggtggatac gacaaacatc ctgtttatct gcggtggtgc ttttgcgggt ctcgaccgta tcatttccgc tcgcggcgag aagacctcca tcggctttgg cgctacggtc aagtccgtcg atgagcgtcg tatcggtgaa gtcttcaagg aattggaacc ggaagacctg ttgaaattcg gcctgattcc ggaatttgtc ggtcgtctgc ctgtcatcgc tacgctcgaa gacctcgacg tcgatgctct ggtccagatt ctgaccgagc cgaagaatgc gctcgtgaag cagtatcagc gcctgttcga catggaaaat gtcgaactgg tgttccatga cgatgcgttg cgcgcgattg ccaacaaggc tgtcgaacgc aagacgggtg cacgcggcct gcgttcgatc atggaaaaga ttctgctcga taccatgttt gaactgccga cgctggaagg cgttcgcgaa gttgtgattt cgggcgatgt ggtggatgga agcgcccgcc cgctatacat caaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for BrabA.00030.a.A4.GE28017
HARVESTED ON: 12/7/2009
SEQUENCED ON: 12/23/2009
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 85
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGVPTPQEI MAVLDDYVIG QKDAKRVLSV AVHNHYKRLA HQSKNSDIEL AKSNILLVGP TGCGKTYLAQ TLARIIDVPF IMADATTLTE AGYVGEDVEN IILKLLQAAD YNVERAQRGI VYIDEVDKIS RKSDNPSITR DVSGEGVQQA LLKIMEGTVA SVPPQGGRKH PQQEFLQVDT TNILFICGGA FAGLDRIISA RGEKTSIGFG ATVKSVDERR IGEVFKELEP EDLLKFGLIP EFVGRLPVIA TLEDLDVDAL VQILTEPKNA LVKQYQRLFD MENXELXXHD DALRAIXNXX VERKXGA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgggag taccgacgcc gcaggaaatc atggccgtcc ttgacgatta cgtcatcggc cagaaggatg ccaagcgcgt tctgtcggtt gcggttcaca atcattacaa gcgtctggcg catcagtcca agaacagcga tatcgaactg gcgaagtcga acattctgct cgtcggcccg acgggctgcg gcaagaccta tcttgcccag acgcttgccc gcatcatcga tgtgccgttc atcatggcgg atgcgacgac gctgacggaa gccggctatg tcggcgaaga tgtggagaac atcatcctga agctgcttca ggccgccgat tataatgtgg agcgcgcgca gcgcggcatc gtctatattg atgaagtgga caagatcagc cgcaagtcgg acaatccatc cattacccgt gacgtatcgg gcgagggtgt gcagcaggcc ctgctgaaga tcatggaagg cactgtcgct tccgtgccgc cgcagggtgg gcgcaagcat ccgcagcagg aattcctgca ggtggatacg acaaacatcc tgtttatctg cggtggtgct tttgcgggtc tcgaccgtat catttccgct cgcggcgaga agacctccat cggctttggc gctacggtca agtccgtcga tgagcgtcgt atcggtgaag tcttcaagga attggaaccg gaagacctgt tgaaattcgg cctgattccg gaatttgtcg gtcgtctgcc tgtcatcgct acgctcgaag acctcgacgt cgatgctctg gtccagattc tgaccgagcc gaagaatgcg ctcgtgaagc agtatcagcg cctgttcgac atggaaaatg ncgaactggn gntccatgac gatgcgttgc gcgcgattgn caacanngnt gtcgaacgca agangggngc ncgc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGVPTPQEI MAVLDDYVIG QKDAKRVLSV AVHNHYKRLA HQSKNSDIEL AKSNILLVGP TGCGKTYLAQ TLARIIDVPF IMADATTLTE AGYVGEDVEN IILKLLQAAD YNVERAQRGI VYIDEVDKIS RKSDNPSITR DVSGEGVQQA LLKIMEGTVA SVPPQGGRKH PQQEFLQVDT TNILFICGGA FAGLDRIISA RGEKTSIGFG ATVKSVDERR IGEVFKELEP EDLLKFGLIP EFVGRLPVIA TLEDLDVDAL VQILTEPKNA LVKQYQRLFD MENVELVFHD DALRAIANKA VERKTGARGL RSIMEKILLD TMFELPTLEG VREVVISGDV VDGSARPLYI YAERQDEKGN VSA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggagtaccg acgccgcagg aaatcatggc cgtccttgac gattacgtca tcggccagaa ggatgccaag cgcgttctgt cggttgcggt tcacaatcat tacaagcgtc tggcgcatca gtccaagaac agcgatatcg aactggcgaa gtcgaacatt ctgctcgtcg gcccgacggg ctgcggcaag acctatcttg cccagacgct tgcccgcatc atcgatgtgc cgttcatcat ggcggatgcg acgacgctga cggaagccgg ctatgtcggc gaagatgtgg agaacatcat cctgaagctg cttcaggccg ccgattataa tgtggagcgc gcgcagcgcg gcatcgtcta tattgatgaa gtggacaaga tcagccgcaa gtcggacaat ccatccatta cccgtgacgt atcgggcgag ggtgtgcagc aggccctgct gaagatcatg gaaggcactg tcgcttccgt gccgccgcag ggtgggcgca agcatccgca gcaggaattc ctgcaggtgg atacgacaaa catcctgttt atctgcggtg gtgcttttgc gggtctcgac cgtatcattt ccgctcgcgg cgagaagacc tccatcggct ttggcgctac ggtcaagtcc gtcgatgagc gtcgtatcgg tgaagtcttc aaggaattgg aaccggaaga cctgttgaaa ttcggcctga ttccggaatt tgtcggtcgt ctgcctgtca tcgctacgct cgaagacctc gacgtcgatg ctctggtcca gattctgacc gagccgaaga atgcgctcgt gaagcagtat cagcgcctgt tcgacatgga aaatgtcgaa ctggtgttcc atgacgatgc gttgcgcgcg attgccaaca aggctgtcga acgcaagacg ggtgcacgcg gcctgcgttc gatcatggaa aagattctgc tcgataccat gtttgaactg ccgacgctgg aaggcgttcg cgaagttgtg atttcgggcg atgtggtgga tggaagcgcc cgcccgctat acatctacgc cgagcggcag gacgaaaagg gcaacgtttc agccaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for BrabA.00030.a.A6.GE28019
HARVESTED ON: 12/7/2009
SEQUENCED ON: 12/23/2009
EXPECTED MW: 40kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGVPTPQEI MAVLDDYVIG QKDAKRVLSV AVHNHYKRLA HQSKNSDIEL AKSNILLVGP TGCGKTYLAQ TLARIIDVPF IMADATTLTE AGYVGEDVEN IILKLLQAAD YNVERAQRGI VYIDEVDKIS RKSDNPSITR DVSGEGVQQA LLKIMEGTVA SVPPQGGRKH PQQEFLQVDT TNILFICGGA FAGLDRIISA RGEKTSIGFG ATVKSVDERR IGEVFKELEP EDLLKFGLIP EFVGRLPVIA TLEDLDVDAL VQILTEPKNA LVKQYQRLFD MENVELVFHD DALRAIANKA VERKTGARGL RSIMEKILLD TMFELPTLEG VREVVISGDV VDGSARPL
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgggag taccgacgcc gcaggaaatc atggccgtcc ttgacgatta cgtcatcggc cagaaggatg ccaagcgcgt tctgtcggtt gcggttcaca atcattacaa gcgtctggcg catcagtcca agaacagcga tatcgaactg gcgaagtcga acattctgct cgtcggcccg acgggctgcg gcaagaccta tcttgcccag acgcttgccc gcatcatcga tgtgccgttc atcatggcgg atgcgacgac gctgacggaa gccggctatg tcggcgaaga tgtggagaac atcatcctga agctgcttca ggccgccgat tataatgtgg agcgcgcgca gcgcggcatc gtctatattg atgaagtgga caagatcagc cgcaagtcgg acaatccatc cattacccgt gacgtatcgg gcgagggtgt gcagcaggcc ctgctgaaga tcatggaagg cactgtcgct tccgtgccgc cgcagggtgg gcgcaagcat ccgcagcagg aattcctgca ggtggatacg acaaacatcc tgtttatctg cggtggtgct tttgcgggtc tcgaccgtat catttccgct cgcggcgaga agacctccat cggctttggc gctacggtca agtccgtcga tgagcgtcgt atcggtgaag tcttcaagga attggaaccg gaagacctgt tgaaattcgg cctgattccg gaatttgtcg gtcgtctgcc tgtcatcgct acgctcgaag acctcgacgt cgatgctctg gtccagattc tgaccgagcc gaagaatgcg ctcgtgaagc agtatcagcg cctgttcgac atggaaaatg tcgaactggt gttccatgac gatgcgttgc gcgcgattgc caacaaggct gtcgaacgca agacgggtgc acgcggcctg cgttcgatca tggaaaagat tctgctcgat accatgtttg aactgccgac gctggaaggc gttcgcgaag ttgtgatttc gggcgatgtg gtggatggaa gcgcccgccc gctat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGVPTPQEI MAVLDDYVIG QKDAKRVLSV AVHNHYKRLA HQSKNSDIEL AKSNILLVGP TGCGKTYLAQ TLARIIDVPF IMADATTLTE AGYVGEDVEN IILKLLQAAD YNVERAQRGI VYIDEVDKIS RKSDNPSITR DVSGEGVQQA LLKIMEGTVA SVPPQGGRKH PQQEFLQVDT TNILFICGGA FAGLDRIISA RGEKTSIGFG ATVKSVDERR IGEVFKELEP EDLLKFGLIP EFVGRLPVIA TLEDLDVDAL VQILTEPKNA LVKQYQRLFD MENVELVFHD DALRAIANKA VERKTGARGL RSIMEKILLD TMFELPTLEG VREVVISGDV VDGSARPLYI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggagtaccg acgccgcagg aaatcatggc cgtccttgac gattacgtca tcggccagaa ggatgccaag cgcgttctgt cggttgcggt tcacaatcat tacaagcgtc tggcgcatca gtccaagaac agcgatatcg aactggcgaa gtcgaacatt ctgctcgtcg gcccgacggg ctgcggcaag acctatcttg cccagacgct tgcccgcatc atcgatgtgc cgttcatcat ggcggatgcg acgacgctga cggaagccgg ctatgtcggc gaagatgtgg agaacatcat cctgaagctg cttcaggccg ccgattataa tgtggagcgc gcgcagcgcg gcatcgtcta tattgatgaa gtggacaaga tcagccgcaa gtcggacaat ccatccatta cccgtgacgt atcgggcgag ggtgtgcagc aggccctgct gaagatcatg gaaggcactg tcgcttccgt gccgccgcag ggtgggcgca agcatccgca gcaggaattc ctgcaggtgg atacgacaaa catcctgttt atctgcggtg gtgcttttgc gggtctcgac cgtatcattt ccgctcgcgg cgagaagacc tccatcggct ttggcgctac ggtcaagtcc gtcgatgagc gtcgtatcgg tgaagtcttc aaggaattgg aaccggaaga cctgttgaaa ttcggcctga ttccggaatt tgtcggtcgt ctgcctgtca tcgctacgct cgaagacctc gacgtcgatg ctctggtcca gattctgacc gagccgaaga atgcgctcgt gaagcagtat cagcgcctgt tcgacatgga aaatgtcgaa ctggtgttcc atgacgatgc gttgcgcgcg attgccaaca aggctgtcga acgcaagacg ggtgcacgcg gcctgcgttc gatcatggaa aagattctgc tcgataccat gtttgaactg ccgacgctgg aaggcgttcg cgaagttgtg atttcgggcg atgtggtgga tggaagcgcc cgcccgctat acatcaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BrabA.00030.a.A3.PS00504
PURIFICATION DATe: 2/25/2010
CONCENTRATION: 17.65mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 100µl
PERCENT IDENTITY: 97
PERCENT COVERAGE: 67
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSKVSNGGG DSKNTLYCSF CGKSQHEVRK LIAGPTVFIC DECVELCMDI IREENKSSMV KSREGVPTPQ EIMAVLDDYV IGQKDAKRVL SVAVHNHYKR LAHQSKNSDI ELAKSNILLV GPTGCGKTYL AQTLARIIDV PFIMADATTL TEAGYVGEDV ENIILKLLQA ADYNVERAQR GIVYIDEVDK ISRKSDNPSI TRDVSGEGVQ QALLKIMEGT VASVPPXGXR KHPQXEFLQV DTTNILFIXG GAFAGLDRII SARGXKXSXG FGA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagca aagtcagcaa cggcggcggc gattccaaga atacgcttta ttgctcgttc tgtggcaaaa gccagcatga agtgcgcaag ctgattgcag gcccgaccgt tttcatctgc gacgaatgcg tcgaactctg catggacatc atccgtgagg aaaacaaatc ctccatggtg aagtcgcgtg agggagtacc gacgccgcag gaaatcatgg ccgtccttga cgattacgtc atcggccaga aggatgccaa gcgcgttctg tcggttgcgg ttcacaatca ttacaagcgt ctggcgcatc agtccaagaa cagcgatatc gaactggcga agtcgaacat tctgctcgtc ggcccgacgg gctgcggcaa gacctatctt gcccagacgc ttgcccgcat catcgatgtg ccgttcatca tggcggatgc gacgacgctg acggaagccg gctatgtcgg cgaagatgtg gagaacatca tcctgaagct gcttcaggcc gccgattata atgtggagcg cgcgcagcgc ggcatcgtct atattgatga agtggacaag atcagccgca agtcggacaa tccatccatt acccgtgacg tatcgggcga gggtgtgcag caggccctgc tgaagatcat ggaaggcact gtcgcttccg tgccgccgca nggtgngcgc aagcatccgc agcangaatt cctgcaggtg gatacnacaa acatcctgtt tatctgnggn ggtgcttttg cgggnctcga ccgtatcatt tccgctcgcg gcganaagan ctccntcggn tttggcgcta c
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSKVSNGGG DSKNTLYCSF CGKSQHEVRK LIAGPTVFIC DECVELCMDI IREENKSSMV KSREGVPTPQ EIMAVLDDYV IGQKDAKRVL SVAVHNHYKR LAHQSKNSDI ELAKSNILLV GPTGCGKTYL AQTLARIIDV PFIMADATTL TEAGYVGEDV ENIILKLLQA ADYNVERAQR GIVYIDEVDK ISRKSDNPSI TRDVSGEGVQ QALLKIMEGT VASVPPQGGR KHPQQEFLQV DTTNILFICG GAFAGLDRII SARGEKTSIG FGATVKSVDE RRIGEVFKEL EPEDLLKFGL IPEFVGRLPV IATLEDLDVD ALVQILTEPK NALVKQYQRL FDMENVELVF HDDALRAIAN KAVERKTGAR GLRSIMEKIL LDTMFELPTL EGVREVVISG DVVDGSARPL YI
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCAAAGTC AGCAACGGCG GCGGCGATTC CAAGAATACG CTTTATTGCT CGTTCTGTGG CAAAAGCCAG CATGAAGTGC GCAAGCTGAT TGCAGGCCCG ACCGTTTTCA TCTGCGACGA ATGCGTCGAA CTCTGCATGG ACATCATCCG TGAGGAAAAC AAATCCTCCA TGGTGAAGTC GCGTGAGGGA GTACCGACGC CGCAGGAAAT CATGGCCGTC CTTGACGATT ACGTCATCGG CCAGAAGGAT GCCAAGCGCG TTCTGTCGGT TGCGGTTCAC AATCATTACA AGCGTCTGGC GCATCAGTCC AAGAACAGCG ATATCGAACT GGCGAAGTCG AACATTCTGC TCGTCGGCCC GACGGGCTGC GGCAAGACCT ATCTTGCCCA GACGCTTGCC CGCATCATCG ATGTGCCGTT CATCATGGCG GATGCGACGA CGCTGACGGA AGCCGGCTAT GTCGGCGAAG ATGTGGAGAA CATCATCCTG AAGCTGCTTC AGGCCGCCGA TTATAATGTG GAGCGCGCGC AGCGCGGCAT CGTCTATATT GATGAAGTGG ACAAGATCAG CCGCAAGTCG GACAATCCAT CCATTACCCG TGACGTATCG GGCGAGGGTG TGCAGCAGGC CCTGCTGAAG ATCATGGAAG GCACTGTCGC TTCCGTGCCG CCGCAGGGTG GGCGCAAGCA TCCGCAGCAG GAATTCCTGC AGGTGGATAC GACAAACATC CTGTTTATCT GCGGTGGTGC TTTTGCGGGT CTCGACCGTA TCATTTCCGC TCGCGGCGAG AAGACCTCCA TCGGCTTTGG CGCTACGGTC AAGTCCGTCG ATGAGCGTCG TATCGGTGAA GTCTTCAAGG AATTGGAACC GGAAGACCTG TTGAAATTCG GCCTGATTCC GGAATTTGTC GGTCGTCTGC CTGTCATCGC TACGCTCGAA GACCTCGACG TCGATGCTCT GGTCCAGATT CTGACCGAGC CGAAGAATGC GCTCGTGAAG CAGTATCAGC GCCTGTTCGA CATGGAAAAT GTCGAACTGG TGTTCCATGA CGATGCGTTG CGCGCGATTG CCAACAAGGC TGTCGAACGC AAGACGGGTG CACGCGGCCT GCGTTCGATC ATGGAAAAGA TTCTGCTCGA TACCATGTTT GAACTGCCGA CGCTGGAAGG CGTTCGCGAA GTTGTGATTT CGGGCGATGT GGTGGATGGA AGCGCCCGCC CGCTATACAT CAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA
Details for BrabA.00030.a.A6.PS00505
PURIFICATION DATe: 3/1/2010
CONCENTRATION: 25.27mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGVPTPQEI MAVLDDYVIG QKDAKRVLSV AVHNHYKRLA HQSKNSDIEL AKSNILLVGP TGCGKTYLAQ TLARIIDVPF IMADATTLTE AGYVGEDVEN IILKLLQAAD YNVERAQRGI VYIDEVDKIS RKSDNPSITR DVSGEGVQQA LLKIMEGTVA SVPPQGGRKH PQQEFLQVDT TNILFICGGA FAGLDRIISA RGEKTSIGFG ATVKSVDERR IGEVFKELEP EDLLKFGLIP EFVGRLPVIA TLEDLDVDAL VQILTEPKNA LVKQYQRLFD MENVELVFHD DALRAIANKA VERKTGARGL RSIMEKILLD TMFELPTLEG VREVVISGDV VDGSARPL
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgggag taccgacgcc gcaggaaatc atggccgtcc ttgacgatta cgtcatcggc cagaaggatg ccaagcgcgt tctgtcggtt gcggttcaca atcattacaa gcgtctggcg catcagtcca agaacagcga tatcgaactg gcgaagtcga acattctgct cgtcggcccg acgggctgcg gcaagaccta tcttgcccag acgcttgccc gcatcatcga tgtgccgttc atcatggcgg atgcgacgac gctgacggaa gccggctatg tcggcgaaga tgtggagaac atcatcctga agctgcttca ggccgccgat tataatgtgg agcgcgcgca gcgcggcatc gtctatattg atgaagtgga caagatcagc cgcaagtcgg acaatccatc cattacccgt gacgtatcgg gcgagggtgt gcagcaggcc ctgctgaaga tcatggaagg cactgtcgct tccgtgccgc cgcagggtgg gcgcaagcat ccgcagcagg aattcctgca ggtggatacg acaaacatcc tgtttatctg cggtggtgct tttgcgggtc tcgaccgtat catttccgct cgcggcgaga agacctccat cggctttggc gctacggtca agtccgtcga tgagcgtcgt atcggtgaag tcttcaagga attggaaccg gaagacctgt tgaaattcgg cctgattccg gaatttgtcg gtcgtctgcc tgtcatcgct acgctcgaag acctcgacgt cgatgctctg gtccagattc tgaccgagcc gaagaatgcg ctcgtgaagc agtatcagcg cctgttcgac atggaaaatg tcgaactggt gttccatgac gatgcgttgc gcgcgattgc caacaaggct gtcgaacgca agacgggtgc acgcggcctg cgttcgatca tggaaaagat tctgctcgat accatgtttg aactgccgac gctggaaggc gttcgcgaag ttgtgatttc gggcgatgtg gtggatggaa gcgcccgccc gctat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGVPTPQEI MAVLDDYVIG QKDAKRVLSV AVHNHYKRLA HQSKNSDIEL AKSNILLVGP TGCGKTYLAQ TLARIIDVPF IMADATTLTE AGYVGEDVEN IILKLLQAAD YNVERAQRGI VYIDEVDKIS RKSDNPSITR DVSGEGVQQA LLKIMEGTVA SVPPQGGRKH PQQEFLQVDT TNILFICGGA FAGLDRIISA RGEKTSIGFG ATVKSVDERR IGEVFKELEP EDLLKFGLIP EFVGRLPVIA TLEDLDVDAL VQILTEPKNA LVKQYQRLFD MENVELVFHD DALRAIANKA VERKTGARGL RSIMEKILLD TMFELPTLEG VREVVISGDV VDGSARPLYI
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGAGTACCG ACGCCGCAGG AAATCATGGC CGTCCTTGAC GATTACGTCA TCGGCCAGAA GGATGCCAAG CGCGTTCTGT CGGTTGCGGT TCACAATCAT TACAAGCGTC TGGCGCATCA GTCCAAGAAC AGCGATATCG AACTGGCGAA GTCGAACATT CTGCTCGTCG GCCCGACGGG CTGCGGCAAG ACCTATCTTG CCCAGACGCT TGCCCGCATC ATCGATGTGC CGTTCATCAT GGCGGATGCG ACGACGCTGA CGGAAGCCGG CTATGTCGGC GAAGATGTGG AGAACATCAT CCTGAAGCTG CTTCAGGCCG CCGATTATAA TGTGGAGCGC GCGCAGCGCG GCATCGTCTA TATTGATGAA GTGGACAAGA TCAGCCGCAA GTCGGACAAT CCATCCATTA CCCGTGACGT ATCGGGCGAG GGTGTGCAGC AGGCCCTGCT GAAGATCATG GAAGGCACTG TCGCTTCCGT GCCGCCGCAG GGTGGGCGCA AGCATCCGCA GCAGGAATTC CTGCAGGTGG ATACGACAAA CATCCTGTTT ATCTGCGGTG GTGCTTTTGC GGGTCTCGAC CGTATCATTT CCGCTCGCGG CGAGAAGACC TCCATCGGCT TTGGCGCTAC GGTCAAGTCC GTCGATGAGC GTCGTATCGG TGAAGTCTTC AAGGAATTGG AACCGGAAGA CCTGTTGAAA TTCGGCCTGA TTCCGGAATT TGTCGGTCGT CTGCCTGTCA TCGCTACGCT CGAAGACCTC GACGTCGATG CTCTGGTCCA GATTCTGACC GAGCCGAAGA ATGCGCTCGT GAAGCAGTAT CAGCGCCTGT TCGACATGGA AAATGTCGAA CTGGTGTTCC ATGACGATGC GTTGCGCGCG ATTGCCAACA AGGCTGTCGA ACGCAAGACG GGTGCACGCG GCCTGCGTTC GATCATGGAA AAGATTCTGC TCGATACCAT GTTTGAACTG CCGACGCTGG AAGGCGTTCG CGAAGTTGTG ATTTCGGGCG ATGTGGTGGA TGGAAGCGCC CGCCCGCTAT ACATCAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA
Details for BrabA.00030.a.A2.PS00506
PURIFICATION DATe: 2/26/2010
CONCENTRATION: 18.95mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSKVSNGGG DSKNTLYCSF CGKSQHEVRK LIAGPTVFIC DECVELCMDI IREENKSSMV KSREGVPTPQ EIMAVLDDYV IGQKDAKRVL SVAVHNHYKR LAHQSKNSDI ELAKSNILLV GPTGCGKTYL AQTLARIIDV PFIMADATTL TEAGYVGEDV ENIILKLLQA ADYNVERAQR GIVYIDEVXK ISRKSDNPSI TRDVSGEGVQ QALLKIMEGT VASVPPQGGR KHPQQEFLQV DTTNILFICG GAFAGLDRII SARGEKTSIG FGATVKSVDE RRIGEVFKEL EPEDLLKFGL IPEFVGRLPV IATLEDLDVD ALVQILTEPK NALVKQYQRL FDMENVELVF HDDALRAIAN KAVERKTGAR GLRSIMEKIL LDTMFELPTL EGVREVVISG DVVDG
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSKVSNGGG DSKNTLYCSF CGKSQHEVRK LIAGPTVFIC DECVELCMDI IREENKSSMV KSREGVPTPQ EIMAVLDDYV IGQKDAKRVL SVAVHNHYKR LAHQSKNSDI ELAKSNILLV GPTGCGKTYL AQTLARIIDV PFIMADATTL TEAGYVGEDV ENIILKLLQA ADYNVERAQR GIVYIDEVDK ISRKSDNPSI TRDVSGEGVQ QALLKIMEGT VASVPPQGGR KHPQQEFLQV DTTNILFICG GAFAGLDRII SARGEKTSIG FGATVKSVDE RRIGEVFKEL EPEDLLKFGL IPEFVGRLPV IATLEDLDVD ALVQILTEPK NALVKQYQRL FDMENVELVF HDDALRAIAN KAVERKTGAR GLRSIMEKIL LDTMFELPTL EGVREVVISG DVVDG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCAAAGTC AGCAACGGCG GCGGCGATTC CAAGAATACG CTTTATTGCT CGTTCTGTGG CAAAAGCCAG CATGAAGTGC GCAAGCTGAT TGCAGGCCCG ACCGTTTTCA TCTGCGACGA ATGCGTCGAA CTCTGCATGG ACATCATCCG TGAGGAAAAC AAATCCTCCA TGGTGAAGTC GCGTGAGGGA GTACCGACGC CGCAGGAAAT CATGGCCGTC CTTGACGATT ACGTCATCGG CCAGAAGGAT GCCAAGCGCG TTCTGTCGGT TGCGGTTCAC AATCATTACA AGCGTCTGGC GCATCAGTCC AAGAACAGCG ATATCGAACT GGCGAAGTCG AACATTCTGC TCGTCGGCCC GACGGGCTGC GGCAAGACCT ATCTTGCCCA GACGCTTGCC CGCATCATCG ATGTGCCGTT CATCATGGCG GATGCGACGA CGCTGACGGA AGCCGGCTAT GTCGGCGAAG ATGTGGAGAA CATCATCCTG AAGCTGCTTC AGGCCGCCGA TTATAATGTG GAGCGCGCGC AGCGCGGCAT CGTCTATATT GATGAAGTGG ACAAGATCAG CCGCAAGTCG GACAATCCAT CCATTACCCG TGACGTATCG GGCGAGGGTG TGCAGCAGGC CCTGCTGAAG ATCATGGAAG GCACTGTCGC TTCCGTGCCG CCGCAGGGTG GGCGCAAGCA TCCGCAGCAG GAATTCCTGC AGGTGGATAC GACAAACATC CTGTTTATCT GCGGTGGTGC TTTTGCGGGT CTCGACCGTA TCATTTCCGC TCGCGGCGAG AAGACCTCCA TCGGCTTTGG CGCTACGGTC AAGTCCGTCG ATGAGCGTCG TATCGGTGAA GTCTTCAAGG AATTGGAACC GGAAGACCTG TTGAAATTCG GCCTGATTCC GGAATTTGTC GGTCGTCTGC CTGTCATCGC TACGCTCGAA GACCTCGACG TCGATGCTCT GGTCCAGATT CTGACCGAGC CGAAGAATGC GCTCGTGAAG CAGTATCAGC GCCTGTTCGA CATGGAAAAT GTCGAACTGG TGTTCCATGA CGATGCGTTG CGCGCGATTG CCAACAAGGC TGTCGAACGC AAGACGGGTG CACGCGGCCT GCGTTCGATC ATGGAAAAGA TTCTGCTCGA TACCATGTTT GAACTGCCGA CGCTGGAAGG CGTTCGCGAA GTTGTGATTT CGGGCGATGT GGTGGATGGA AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA
Details for BrabA.00030.a.A4.PS00508
PURIFICATION DATe: 2/24/2010
CONCENTRATION: 20.4mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 11
VIAL VOLUME: 100µl
PERCENT IDENTITY: 97
PERCENT COVERAGE: 85
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGVPTPQEI MAVLDDYVIG QKDAKRVLSV AVHNHYKRLA HQSKNSDIEL AKSNILLVGP TGCGKTYLAQ TLARIIDVPF IMADATTLTE AGYVGEDVEN IILKLLQAAD YNVERAQRGI VYIDEVDKIS RKSDNPSITR DVSGEGVQQA LLKIMEGTVA SVPPQGGRKH PQQEFLQVDT TNILFICGGA FAGLDRIISA RGEKTSIGFG ATVKSVDERR IGEVFKELEP EDLLKFGLIP EFVGRLPVIA TLEDLDVDAL VQILTEPKNA LVKQYQRLFD MENXELXXHD DALRAIXNXX VERKXGA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgggag taccgacgcc gcaggaaatc atggccgtcc ttgacgatta cgtcatcggc cagaaggatg ccaagcgcgt tctgtcggtt gcggttcaca atcattacaa gcgtctggcg catcagtcca agaacagcga tatcgaactg gcgaagtcga acattctgct cgtcggcccg acgggctgcg gcaagaccta tcttgcccag acgcttgccc gcatcatcga tgtgccgttc atcatggcgg atgcgacgac gctgacggaa gccggctatg tcggcgaaga tgtggagaac atcatcctga agctgcttca ggccgccgat tataatgtgg agcgcgcgca gcgcggcatc gtctatattg atgaagtgga caagatcagc cgcaagtcgg acaatccatc cattacccgt gacgtatcgg gcgagggtgt gcagcaggcc ctgctgaaga tcatggaagg cactgtcgct tccgtgccgc cgcagggtgg gcgcaagcat ccgcagcagg aattcctgca ggtggatacg acaaacatcc tgtttatctg cggtggtgct tttgcgggtc tcgaccgtat catttccgct cgcggcgaga agacctccat cggctttggc gctacggtca agtccgtcga tgagcgtcgt atcggtgaag tcttcaagga attggaaccg gaagacctgt tgaaattcgg cctgattccg gaatttgtcg gtcgtctgcc tgtcatcgct acgctcgaag acctcgacgt cgatgctctg gtccagattc tgaccgagcc gaagaatgcg ctcgtgaagc agtatcagcg cctgttcgac atggaaaatg ncgaactggn gntccatgac gatgcgttgc gcgcgattgn caacanngnt gtcgaacgca agangggngc ncgc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGVPTPQEI MAVLDDYVIG QKDAKRVLSV AVHNHYKRLA HQSKNSDIEL AKSNILLVGP TGCGKTYLAQ TLARIIDVPF IMADATTLTE AGYVGEDVEN IILKLLQAAD YNVERAQRGI VYIDEVDKIS RKSDNPSITR DVSGEGVQQA LLKIMEGTVA SVPPQGGRKH PQQEFLQVDT TNILFICGGA FAGLDRIISA RGEKTSIGFG ATVKSVDERR IGEVFKELEP EDLLKFGLIP EFVGRLPVIA TLEDLDVDAL VQILTEPKNA LVKQYQRLFD MENVELVFHD DALRAIANKA VERKTGARGL RSIMEKILLD TMFELPTLEG VREVVISGDV VDGSARPLYI YAERQDEKGN VSA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGAGTACCG ACGCCGCAGG AAATCATGGC CGTCCTTGAC GATTACGTCA TCGGCCAGAA GGATGCCAAG CGCGTTCTGT CGGTTGCGGT TCACAATCAT TACAAGCGTC TGGCGCATCA GTCCAAGAAC AGCGATATCG AACTGGCGAA GTCGAACATT CTGCTCGTCG GCCCGACGGG CTGCGGCAAG ACCTATCTTG CCCAGACGCT TGCCCGCATC ATCGATGTGC CGTTCATCAT GGCGGATGCG ACGACGCTGA CGGAAGCCGG CTATGTCGGC GAAGATGTGG AGAACATCAT CCTGAAGCTG CTTCAGGCCG CCGATTATAA TGTGGAGCGC GCGCAGCGCG GCATCGTCTA TATTGATGAA GTGGACAAGA TCAGCCGCAA GTCGGACAAT CCATCCATTA CCCGTGACGT ATCGGGCGAG GGTGTGCAGC AGGCCCTGCT GAAGATCATG GAAGGCACTG TCGCTTCCGT GCCGCCGCAG GGTGGGCGCA AGCATCCGCA GCAGGAATTC CTGCAGGTGG ATACGACAAA CATCCTGTTT ATCTGCGGTG GTGCTTTTGC GGGTCTCGAC CGTATCATTT CCGCTCGCGG CGAGAAGACC TCCATCGGCT TTGGCGCTAC GGTCAAGTCC GTCGATGAGC GTCGTATCGG TGAAGTCTTC AAGGAATTGG AACCGGAAGA CCTGTTGAAA TTCGGCCTGA TTCCGGAATT TGTCGGTCGT CTGCCTGTCA TCGCTACGCT CGAAGACCTC GACGTCGATG CTCTGGTCCA GATTCTGACC GAGCCGAAGA ATGCGCTCGT GAAGCAGTAT CAGCGCCTGT TCGACATGGA AAATGTCGAA CTGGTGTTCC ATGACGATGC GTTGCGCGCG ATTGCCAACA AGGCTGTCGA ACGCAAGACG GGTGCACGCG GCCTGCGTTC GATCATGGAA AAGATTCTGC TCGATACCAT GTTTGAACTG CCGACGCTGG AAGGCGTTCG CGAAGTTGTG ATTTCGGGCG ATGTGGTGGA TGGAAGCGCC CGCCCGCTAT ACATCTACGC CGAGCGGCAG GACGAAAAGG GCAACGTTTC AGCCAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA