BrabA.00028.a
Ferritin:Bacterioferritin
| CENTER ID: | BrabA.00028.a |
| ORGANISM: | Brucella melitensis biovar Abortus 2308 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.00028.a.A1.GE25589 | Full length( BrabA.00028.a ) | 1 | 161 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
3FVB
DEPOSITED: 1/15/2009
DETERMINATION: XRay
CLONE: BrabA.00028.a.A1.GE25589
PROTEIN: BrabA.00028.a.A1.PW25634
8SQP
DEPOSITED: 5/4/2023
DETERMINATION: XRay
CLONE: BrabA.00028.a.A1.GE25589
PROTEIN: BrabA.00028.a.A1.PW39164
8SQQ
DEPOSITED: 5/4/2023
DETERMINATION: XRay
CLONE: BrabA.00028.a.A1.GE25589
PROTEIN: BrabA.00028.a.A1.PW39165
8SQO
DEPOSITED: 5/4/2023
DETERMINATION: XRay
CLONE: BrabA.00028.a.A1.GE25589
PROTEIN: BrabA.00028.a.A1.PW39164
8SQR
DEPOSITED: 5/4/2023
DETERMINATION: XRay
CLONE: BrabA.00028.a.A1.GE25589
PROTEIN: BrabA.00028.a.A1.PW39164
8SQT
DEPOSITED: 5/4/2023
DETERMINATION: XRay
CLONE: BrabA.00028.a.A1.GE25589
PROTEIN: BrabA.00028.a.A1.PW39164
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_135716 |
| PATRIC ID: | fig|359391.4.peg.2971 |
| RefSeq: | YP_418850 |
| UniProt: | Q2YKI4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKGEPKVIER LNEALFLELG AVNQYWLHYR LLNDWGYTRL AKKEREESIE EMHHADKLID RIIFLEGFPN LQTVSPLRIG QNVKEVLEAD LKGEYDARAS YKESREICDK LGDYVSKQLF DELLADEEGH IDFLETQLDL LAKIGGERYG QLNAAPADEA E
NT Sequence
ATGAAAGGCG AACCAAAGGT CATCGAGCGG CTTAACGAGG CACTGTTTCT TGAGCTCGGT GCGGTAAACC AGTATTGGCT GCACTACCGT CTTCTCAACG ATTGGGGTTA CACGCGCCTT GCAAAGAAGG AACGCGAGGA ATCCATCGAG GAAATGCATC ACGCCGACAA GCTGATTGAT CGCATTATCT TCCTTGAAGG CTTTCCGAAC CTCCAGACCG TTTCGCCGTT GCGCATTGGC CAGAATGTGA AGGAAGTTCT CGAAGCTGAC CTCAAGGGTG AATATGACGC TCGCGCTTCG TATAAGGAAT CGCGCGAAAT CTGCGACAAG CTCGGCGACT ATGTGTCGAA GCAGCTTTTC GACGAACTTC TGGCCGATGA AGAAGGCCAT ATCGACTTCC TTGAAACCCA GCTTGACCTT CTCGCCAAGA TCGGCGGAGA ACGCTATGGC CAGCTTAACG CGGCGCCCGC CGACGAAGCT GAG
Details for BrabA.00028.a.A1.GE25589
| HARVESTED ON: | 9/10/2008 |
| SEQUENCED ON: | 5/1/2015 |
| EXPECTED MW: | 20kDa |
| OBSERVED MW: | 21kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKGEPKVIE RLNEALFLEL GAVNQYWLHY RLLNDWGYTR LAKKEREESI EEMHHADKLI DRIIFLEGFP NLQTVSPLRI GQNVKEVLEA DLKGEYDARA SYKESREICD KLGDYVSKQL FDELLADEEG HIDFLETQLD LLAKIGGERY GQLNAAPADE AE
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaag gcgaaccaaa ggtcatcgag cggcttaacg aggcactgtt tcttgagctc ggtgcggtaa accagtattg gctgcactac cgtcttctca acgattgggg ttacacgcgc cttgcaaaga aggaacgcga ggaatccatc gaggaaatgc atcacgccga caagctgatt gatcgcatta tcttccttga aggctttccg aacctccaga ccgtttcgcc gttgcgcatt ggccagaatg tgaaggaagt tctcgaagct gacctcaagg gtgaatatga cgctcgcgct tcgtataagg aatcgcgcga aatctgcgac aagctcggcg actatgtgtc gaagcagctt ttcgacgaac ttctggccga tgaagaaggc catatcgact tccttgaaac ccagcttgac cttctcgcca agatcggcgg agaacgctat ggccagctta acgcggcgcc cgccgacgaa gctgagtaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKGEPKVIE RLNEALFLEL GAVNQYWLHY RLLNDWGYTR LAKKEREESI EEMHHADKLI DRIIFLEGFP NLQTVSPLRI GQNVKEVLEA DLKGEYDARA SYKESREICD KLGDYVSKQL FDELLADEEG HIDFLETQLD LLAKIGGERY GQLNAAPADE AE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaaggcgaa ccaaaggtca tcgagcggct taacgaggca ctgtttcttg agctcggtgc ggtaaaccag tattggctgc actaccgtct tctcaacgat tggggttaca cgcgccttgc aaagaaggaa cgcgaggaat ccatcgagga aatgcatcac gccgacaagc tgattgatcg cattatcttc cttgaaggct ttccgaacct ccagaccgtt tcgccgttgc gcattggcca gaatgtgaag gaagttctcg aagctgacct caagggtgaa tatgacgctc gcgcttcgta taaggaatcg cgcgaaatct gcgacaagct cggcgactat gtgtcgaagc agcttttcga cgaacttctg gccgatgaag aaggccatat cgacttcctt gaaacccagc ttgaccttct cgccaagatc ggcggagaac gctatggcca gcttaacgcg gcgcccgccg acgaagctga gtaaaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa