BrabA.00027.c

Butyryl-CoA dehydrogenase (EC 1.3.99.2)

CENTER ID: BrabA.00027.c
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.00027.c.B1.GE37694 Full length( BrabA.00027.c ) 1 385
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.00027.c.B1.PS02049 Full length( BrabA.00027.c ) 1 385

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruMel86222_1387
BV-BRC: fig|359391.4.peg.1387
RefSeq: YP_414712.1
UniProt: Q2YQK0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDTADQNQFE LNEDQCAIQE MAQAFAADRI APQALQWDRD KHFPVDILRE TGPLGMGGIY VRDDVGGSGL KRLDAVLIFE ALATACPTFS AFLSIHNMAA WMIDTFGNEE QRQRFLPQLT SMEWLASYCL TEPGSGSDAA ALKTRAVRDG DHYIVNGAKQ FISGAGSTDL YVTMVRTGED GPKGISTLVV PKDAPGLSFG ANEYKMGWNA QPTRTVIFDN CRVPVENRLG DEGVGFKIAM PGLDGGRLNI AACSLGGAQA ATAKALEYCA ERKAFGQTID RFQALQFRLA DMETELAASR MLLYTAASKL DRKTHDAGKW SAMAKRFVTD TSFNVANEAL QLLGGYGYLH DYGIEKLVRD LRVHQILEGT NEIMRVIIAR HMIGR
NT Sequence
ATGGATACTG CCGATCAGAA CCAGTTTGAA CTGAATGAGG ATCAATGCGC CATTCAGGAG ATGGCGCAGG CCTTTGCCGC CGACCGGATC GCACCGCAGG CCCTGCAATG GGACCGCGAC AAGCATTTCC CCGTCGATAT CCTGCGTGAG ACAGGGCCGC TCGGCATGGG CGGCATTTAC GTGCGCGACG ATGTGGGTGG ATCGGGGCTG AAACGGCTTG ATGCGGTGCT TATTTTTGAG GCGCTCGCCA CGGCTTGCCC GACATTCTCG GCCTTTCTGT CCATCCATAA CATGGCCGCA TGGATGATCG ACACCTTTGG CAATGAGGAA CAGCGCCAGC GCTTCCTGCC GCAACTGACT TCCATGGAAT GGCTGGCAAG CTATTGCCTG ACGGAGCCGG GTTCGGGTTC CGACGCGGCA GCACTCAAGA CCCGCGCCGT GCGCGACGGC GACCATTATA TCGTCAACGG CGCAAAGCAG TTCATATCGG GTGCGGGTTC CACCGATCTC TATGTTACCA TGGTGCGTAC CGGCGAGGAC GGCCCAAAAG GCATTTCCAC GCTCGTTGTG CCTAAAGATG CACCGGGCCT GTCTTTCGGC GCCAATGAAT ATAAAATGGG CTGGAATGCC CAGCCGACCC GCACGGTTAT TTTTGACAAC TGCCGCGTGC CGGTGGAAAA TCGTTTGGGC GATGAAGGCG TCGGCTTCAA AATCGCCATG CCGGGCCTTG ATGGCGGGCG CCTCAATATT GCGGCCTGTT CGCTTGGCGG TGCGCAAGCT GCAACGGCGA AAGCGCTGGA ATATTGCGCC GAGCGCAAGG CTTTCGGACA AACGATCGAT CGTTTTCAGG CCTTGCAGTT CCGCCTAGCC GATATGGAAA CAGAATTGGC CGCCTCGCGC ATGCTTCTTT ATACGGCCGC AAGCAAGCTC GACCGCAAGA CCCATGACGC AGGCAAATGG TCAGCCATGG CGAAACGCTT CGTGACCGAT ACGAGCTTCA ATGTTGCCAA CGAAGCCCTC CAGCTTCTGG GCGGTTACGG CTATCTTCAC GATTATGGCA TCGAGAAACT GGTGCGCGAT CTGCGCGTTC ATCAAATTCT TGAAGGCACC AACGAAATCA TGCGCGTCAT CATCGCCCGT CACATGATTG GCCGT
Details for BrabA.00027.c.B1.GE37694
HARVESTED ON: 2/5/2014
SEQUENCED ON: 2/6/2014
EXPECTED MW: 43kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 90
Validated AA Sequence
MAQAFAADRI APQALQWDRD KHFPVDILRE TGPLGMGGIY VRDDVGGSGL KRLDAVLIFE ALATACPTFS AFLSIHNMAA WMIDTFGNEE QRQRFLPQLT SMEWLASYCL TEPGSGSDAA ALKTRAVRDG DHYIVNGAKQ FISGAGSTDL YVTMVRTGED GPKGISTLVV PKDAPGLSFG ANEYKMGWNA QPTRTVIFDN CRVPVENRLG DEGVGFKIAM PGLDGGRLNI AACSLGGAQA ATAKALEYCA ERKAFGQTID RFQALQFRLA DMETELAASR MLLYTAASKL DRKTHDAGKW SAMAKRFVTD TSFNVANEAL QLLGGYGYLH DYGIEKLVRD LRVHQILEGT NEIMR
Validated NT Sequence
acgcgcatga tttcgttggt gccttcaaga atttgatgaa cgcgcagatc gcgcaccagt ttctcgatgc cataatcgtg aagatagccg taaccgccca gaagctggag ggcttcgttg gcaacattga agctcgtatc ggtcacgaag cgtttcgcca tggctgacca tttgcctgcg tcatgggtct tgcggtcgag cttgcttgcg gccgtataaa gaagcatgcg cgaggcggcc aattctgttt ccatatcggc taggcggaac tgcaaggcct gaaaacgatc gatcgtttgt ccgaaagcct tgcgctcggc gcaatattcc agcgctttcg ccgttgcagc ttgcgcaccg ccaagcgaac aggccgcaat attgaggcgc ccgccatcaa ggcccggcat ggcgattttg aagccgacgc cttcatcgcc caaacgattt tccaccggca cgcggcagtt gtcaaaaata accgtgcggg tcggctgggc attccagccc attttatatt cattggcgcc gaaagacagg cccggtgcat ctttaggcac aacgagcgtg gaaatgcctt ttgggccgtc ctcgccggta cgcaccatgg taacatagag atcggtggaa cccgcacccg atatgaactg ctttgcgccg ttgacgatat aatggtcgcc gtcgcgcacg gcgcgggtct tgagtgctgc cgcgtcggaa cccgaacccg gctccgtcag gcaatagctt gccagccatt ccatggaagt cagttgcggc aggaagcgct ggcgctgttc ctcattgcca aaggtgtcga tcatccatgc ggccatgtta tggatggaca gaaaggccga gaatgtcggg caagccgtgg cgagcgcctc aaaaataagc accgcatcaa gccgtttcag ccccgatcca cccacatcgt cgcgcacgta aatgccgccc atgccgagcg gccctgtctc acgcaggata tcgacgggga aatgcttgtc gcggtcccat tgcagggcct gcggtgcgat ccggtcggcg gcaaaggcct gcgccatctc ctgaatggcg cattgatcct cattcagttc aaactggttc tgatcggca
Expected Protein Sequence
MAHHHHHHMD TADQNQFELN EDQCAIQEMA QAFAADRIAP QALQWDRDKH FPVDILRETG PLGMGGIYVR DDVGGSGLKR LDAVLIFEAL ATACPTFSAF LSIHNMAAWM IDTFGNEEQR QRFLPQLTSM EWLASYCLTE PGSGSDAAAL KTRAVRDGDH YIVNGAKQFI SGAGSTDLYV TMVRTGEDGP KGISTLVVPK DAPGLSFGAN EYKMGWNAQP TRTVIFDNCR VPVENRLGDE GVGFKIAMPG LDGGRLNIAA CSLGGAQAAT AKALEYCAER KAFGQTIDRF QALQFRLADM ETELAASRML LYTAASKLDR KTHDAGKWSA MAKRFVTDTS FNVANEALQL LGGYGYLHDY GIEKLVRDLR VHQILEGTNE IMRVIIARHM IGR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggata ctgccgatca gaaccagttt gaactgaatg aggatcaatg cgccattcag gagatggcgc aggcctttgc cgccgaccgg atcgcaccgc aggccctgca atgggaccgc gacaagcatt tccccgtcga tatcctgcgt gagacagggc cgctcggcat gggcggcatt tacgtgcgcg acgatgtggg tggatcgggg ctgaaacggc ttgatgcggt gcttattttt gaggcgctcg ccacggcttg cccgacattc tcggcctttc tgtccatcca taacatggcc gcatggatga tcgacacctt tggcaatgag gaacagcgcc agcgcttcct gccgcaactg acttccatgg aatggctggc aagctattgc ctgacggagc cgggttcggg ttccgacgcg gcagcactca agacccgcgc cgtgcgcgac ggcgaccatt atatcgtcaa cggcgcaaag cagttcatat cgggtgcggg ttccaccgat ctctatgtta ccatggtgcg taccggcgag gacggcccaa aaggcatttc cacgctcgtt gtgcctaaag atgcaccggg cctgtctttc ggcgccaatg aatataaaat gggctggaat gcccagccga cccgcacggt tatttttgac aactgccgcg tgccggtgga aaatcgtttg ggcgatgaag gcgtcggctt caaaatcgcc atgccgggcc ttgatggcgg gcgcctcaat attgcggcct gttcgcttgg cggtgcgcaa gctgcaacgg cgaaagcgct ggaatattgc gccgagcgca aggctttcgg acaaacgatc gatcgttttc aggccttgca gttccgccta gccgatatgg aaacagaatt ggccgcctcg cgcatgcttc tttatacggc cgcaagcaag ctcgaccgca agacccatga cgcaggcaaa tggtcagcca tggcgaaacg cttcgtgacc gatacgagct tcaatgttgc caacgaagcc ctccagcttc tgggcggtta cggctatctt cacgattatg gcatcgagaa actggtgcgc gatctgcgcg ttcatcaaat tcttgaaggc accaacgaaa tcatgcgcgt catcatcgcc cgtcacatga ttggccgttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for BrabA.00027.c.B1.PS02049
PURIFICATION DATe: 4/30/2014
CONCENTRATION: 33.5mg/ml
OBSERVED MW: 42kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 90
Protocol Notes
notes unavailable
Validated AA Sequence
MAQAFAADRI APQALQWDRD KHFPVDILRE TGPLGMGGIY VRDDVGGSGL KRLDAVLIFE ALATACPTFS AFLSIHNMAA WMIDTFGNEE QRQRFLPQLT SMEWLASYCL TEPGSGSDAA ALKTRAVRDG DHYIVNGAKQ FISGAGSTDL YVTMVRTGED GPKGISTLVV PKDAPGLSFG ANEYKMGWNA QPTRTVIFDN CRVPVENRLG DEGVGFKIAM PGLDGGRLNI AACSLGGAQA ATAKALEYCA ERKAFGQTID RFQALQFRLA DMETELAASR MLLYTAASKL DRKTHDAGKW SAMAKRFVTD TSFNVANEAL QLLGGYGYLH DYGIEKLVRD LRVHQILEGT NEIMR
Validated NT Sequence
acgcgcatga tttcgttggt gccttcaaga atttgatgaa cgcgcagatc gcgcaccagt ttctcgatgc cataatcgtg aagatagccg taaccgccca gaagctggag ggcttcgttg gcaacattga agctcgtatc ggtcacgaag cgtttcgcca tggctgacca tttgcctgcg tcatgggtct tgcggtcgag cttgcttgcg gccgtataaa gaagcatgcg cgaggcggcc aattctgttt ccatatcggc taggcggaac tgcaaggcct gaaaacgatc gatcgtttgt ccgaaagcct tgcgctcggc gcaatattcc agcgctttcg ccgttgcagc ttgcgcaccg ccaagcgaac aggccgcaat attgaggcgc ccgccatcaa ggcccggcat ggcgattttg aagccgacgc cttcatcgcc caaacgattt tccaccggca cgcggcagtt gtcaaaaata accgtgcggg tcggctgggc attccagccc attttatatt cattggcgcc gaaagacagg cccggtgcat ctttaggcac aacgagcgtg gaaatgcctt ttgggccgtc ctcgccggta cgcaccatgg taacatagag atcggtggaa cccgcacccg atatgaactg ctttgcgccg ttgacgatat aatggtcgcc gtcgcgcacg gcgcgggtct tgagtgctgc cgcgtcggaa cccgaacccg gctccgtcag gcaatagctt gccagccatt ccatggaagt cagttgcggc aggaagcgct ggcgctgttc ctcattgcca aaggtgtcga tcatccatgc ggccatgtta tggatggaca gaaaggccga gaatgtcggg caagccgtgg cgagcgcctc aaaaataagc accgcatcaa gccgtttcag ccccgatcca cccacatcgt cgcgcacgta aatgccgccc atgccgagcg gccctgtctc acgcaggata tcgacgggga aatgcttgtc gcggtcccat tgcagggcct gcggtgcgat ccggtcggcg gcaaaggcct gcgccatctc ctgaatggcg cattgatcct cattcagttc aaactggttc tgatcggca
Expressed Protein Sequence
MAHHHHHHMD TADQNQFELN EDQCAIQEMA QAFAADRIAP QALQWDRDKH FPVDILRETG PLGMGGIYVR DDVGGSGLKR LDAVLIFEAL ATACPTFSAF LSIHNMAAWM IDTFGNEEQR QRFLPQLTSM EWLASYCLTE PGSGSDAAAL KTRAVRDGDH YIVNGAKQFI SGAGSTDLYV TMVRTGEDGP KGISTLVVPK DAPGLSFGAN EYKMGWNAQP TRTVIFDNCR VPVENRLGDE GVGFKIAMPG LDGGRLNIAA CSLGGAQAAT AKALEYCAER KAFGQTIDRF QALQFRLADM ETELAASRML LYTAASKLDR KTHDAGKWSA MAKRFVTDTS FNVANEALQL LGGYGYLHDY GIEKLVRDLR VHQILEGTNE IMRVIIARHM IGR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGATA CTGCCGATCA GAACCAGTTT GAACTGAATG AGGATCAATG CGCCATTCAG GAGATGGCGC AGGCCTTTGC CGCCGACCGG ATCGCACCGC AGGCCCTGCA ATGGGACCGC GACAAGCATT TCCCCGTCGA TATCCTGCGT GAGACAGGGC CGCTCGGCAT GGGCGGCATT TACGTGCGCG ACGATGTGGG TGGATCGGGG CTGAAACGGC TTGATGCGGT GCTTATTTTT GAGGCGCTCG CCACGGCTTG CCCGACATTC TCGGCCTTTC TGTCCATCCA TAACATGGCC GCATGGATGA TCGACACCTT TGGCAATGAG GAACAGCGCC AGCGCTTCCT GCCGCAACTG ACTTCCATGG AATGGCTGGC AAGCTATTGC CTGACGGAGC CGGGTTCGGG TTCCGACGCG GCAGCACTCA AGACCCGCGC CGTGCGCGAC GGCGACCATT ATATCGTCAA CGGCGCAAAG CAGTTCATAT CGGGTGCGGG TTCCACCGAT CTCTATGTTA CCATGGTGCG TACCGGCGAG GACGGCCCAA AAGGCATTTC CACGCTCGTT GTGCCTAAAG ATGCACCGGG CCTGTCTTTC GGCGCCAATG AATATAAAAT GGGCTGGAAT GCCCAGCCGA CCCGCACGGT TATTTTTGAC AACTGCCGCG TGCCGGTGGA AAATCGTTTG GGCGATGAAG GCGTCGGCTT CAAAATCGCC ATGCCGGGCC TTGATGGCGG GCGCCTCAAT ATTGCGGCCT GTTCGCTTGG CGGTGCGCAA GCTGCAACGG CGAAAGCGCT GGAATATTGC GCCGAGCGCA AGGCTTTCGG ACAAACGATC GATCGTTTTC AGGCCTTGCA GTTCCGCCTA GCCGATATGG AAACAGAATT GGCCGCCTCG CGCATGCTTC TTTATACGGC CGCAAGCAAG CTCGACCGCA AGACCCATGA CGCAGGCAAA TGGTCAGCCA TGGCGAAACG CTTCGTGACC GATACGAGCT TCAATGTTGC CAACGAAGCC CTCCAGCTTC TGGGCGGTTA CGGCTATCTT CACGATTATG GCATCGAGAA ACTGGTGCGC GATCTGCGCG TTCATCAAAT TCTTGAAGGC ACCAACGAAA TCATGCGCGT CATCATCGCC CGTCACATGA TTGGCCGTTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT