BrabA.00027.a

Acyl-CoA dehydrogenase:Acyl-CoA dehydrogenase, C-terminal:Acyl-CoA dehydrogenase, central domain:Acyl-CoA dehydrogenase, N-te...

CENTER ID: BrabA.00027.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: in PDB
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BrabA.00027.a.A1.GE25216 Full length( BrabA.00027.a ) 1 394
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BrabA.00027.a.D11.PD00015 Full length( BrabA.00027.a ) 1 394
BrabA.00027.a.A1.PS00026 Full length( BrabA.00027.a ) 1 394
BrabA.00027.a.A1.PW26983 Full length( BrabA.00027.a ) 1 394
Structures
4W9U
DEPOSITED: 8/27/2014
DETERMINATION: XRay
CLONE:
PROTEIN: BrabA.00027.a.D11.PD00015
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_129299
PATRIC ID: fig|359391.4.peg.1157
RefSeq: YP_414502
UniProt: Q2YPZ4
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRAAFAWED PFLLEEQLTE DERMIRDSAK AFASDVLLPR VEKAYLEETT DPELFHLMGQ AGLLGVTLPE DYGAANASYV AYGLVAREVE RIDSGYRSMM SVQSSLVMYP IYAYGSDEQR KKYLPGLVSG ELIGCFGLTE PDAGSDPAGM KTRAEKIDGG YRLSGSKMWI SNSPIADVFV VWAKSAAHDN AIRGFILEKG MKGLSAPKIG GKLSLRASIT GEIVMDGVEV SEDAILPNVS GLKGPFGCLN RARYGISWGV LGAAEDCWFR ARQYGLDRKQ FNKPLAGTQL YQKKLADMQT EIALGIQASL RVGRLFDEGK MAPEMISIVK RNNCGKALDI ARQARDMHGG NGIQIEYHVM RHAQNLETVN TYEGTHDVHA LILGRAQTGI QAFF
NT Sequence
ATGTCACGTG CGGCTTTTGC CTGGGAAGAT CCGTTTTTGC TGGAGGAGCA ACTGACCGAA GACGAGCGCA TGATCCGCGA CTCGGCCAAG GCCTTTGCAA GCGACGTTCT TCTGCCTCGT GTCGAAAAAG CCTATCTGGA AGAAACCACC GACCCGGAAC TGTTTCACCT CATGGGGCAG GCGGGGCTTC TGGGCGTAAC GCTGCCGGAA GACTATGGCG CGGCCAATGC AAGCTATGTA GCCTATGGGC TGGTGGCGCG CGAAGTGGAG CGCATTGATT CCGGATATCG TTCCATGATG AGCGTGCAAT CGTCGCTCGT CATGTATCCG ATCTATGCCT ATGGTTCGGA TGAACAGCGC AAGAAATATC TTCCCGGCCT GGTTTCGGGT GAATTGATTG GCTGTTTCGG CCTTACCGAA CCCGATGCGG GTTCCGACCC GGCTGGCATG AAAACCCGCG CCGAAAAGAT CGATGGCGGC TATCGCCTCA GCGGCTCGAA GATGTGGATT TCCAATTCAC CGATTGCCGA TGTGTTCGTC GTCTGGGCAA AATCGGCTGC GCATGACAAC GCCATTCGCG GCTTCATTCT TGAAAAGGGC ATGAAGGGAC TTTCAGCACC GAAGATCGGG GGCAAGCTTT CGCTGCGCGC TTCTATCACC GGCGAGATCG TGATGGATGG GGTTGAAGTT TCCGAAGACG CAATCCTGCC GAATGTTTCC GGTCTCAAGG GCCCGTTTGG CTGTCTCAAC CGCGCGCGCT ATGGCATTTC GTGGGGTGTG CTCGGGGCTG CTGAAGATTG CTGGTTCCGT GCGCGCCAAT ATGGTCTGGA CCGCAAGCAA TTCAACAAGC CGCTGGCTGG CACGCAGCTT TACCAGAAGA AGCTTGCCGA TATGCAGACG GAAATCGCGC TTGGGATTCA GGCATCGCTT CGCGTTGGCC GCCTGTTCGA TGAAGGCAAG ATGGCGCCCG AAATGATTTC TATCGTCAAG CGCAACAATT GCGGCAAGGC TCTGGATATT GCCCGCCAGG CCCGTGATAT GCATGGCGGC AACGGTATCC AGATTGAATA TCACGTCATG CGCCACGCGC AGAATCTGGA AACCGTCAAC ACCTATGAGG GCACGCACGA CGTTCACGCG CTGATTCTCG GCCGTGCCCA GACCGGCATT CAGGCGTTCT TC
Details for BrabA.00027.a.A1.GE25216
HARVESTED ON: 5/6/2008
SEQUENCED ON: 6/16/2009
EXPECTED MW: 45kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSRAAFAWE DPFLLEEQLT EDERMIRDSA KAFASDVLLP RVEKAYLEET TDPELFHLMG QAGLLGVTLP EDYGAANASY VAYGLVAREV ERIDSGYRSM MSVQSSLVMY PIYAYGSDEQ RKKYLPGLVS GELIGCFGLT EPDAGSDPAG MKTRAEKIDG GYRLSGSKMW ISNSPIADVF VVWAKSAAHD NAIRGFILEK GMKGLSAPKI GGKLSLRASI TGEIVMDGVE VSEDAILPNV SGLKGPFGCL NRARYGISWG VLGAAEDCWF RARQYGLXXK QFNKPLAGTQ LYQKKLADMQ TEIALGIQAS LRVGRLFDEG KMAPEMISIV KRNNCGKALD IARQARDMHG GNGIQIEYHV MRHAQNLETV NTYEGTHDXH ALILGRAQTG IQAFF
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtcac gtgcggcttt tgcctgggaa gatccgtttt tgctggagga gcaactgacc gaagacgagc gcatgatccg cgactcggcc aaggcctttg caagcgacgt tcttctgcct cgtgtcgaaa aagcctatct ggaagaaacc accgacccgg aactgtttca cctcatgggg caggcggggc ttctgggcgt aacgctgccg gaagactatg gcgcggccaa tgcaagctat gtagcctatg ggctggtggc gcgcgaagtg gagcgcattg attccggata tcgttccatg atgagcgtgc aatcgtcgct cgtcatgtat ccgatctatg cctatggttc ggatgaacag cgcaagaaat atcttcccgg cctggtttcg ggtgaattga ttggctgttt cggccttacc gaacccgatg cgggttccga cccggctggc atgaaaaccc gcgccgaaaa gatcgatggc ggctatcgcc tcagcggctc gaagatgtgg atttccaatt caccgattgc cgatgtgttc gtcgtctggg caaaatcggc tgcgcatgac aacgccattc gcggcttcat tcttgaaaag ggcatgaagg gactttcagc accgaagatc gggggcaagc tgtcgctgcg cgcttctatc accggcgaga tcgtgatgga tggggttgaa gtttccgaag acgcaatcct gccgaatgtt tccggtctca agggcccgtt tggctgtctc aaccgcgcgc gctatggcat ttcgtggggt gtgctcgggg ctgctgaaga ttgctggttc cgtgcgcgcc aatatggtct gganngcaag caattcaaca agccgctggc tggcacgcag ctttaccaga agaagctggc cgatatgcag acggaaatcg cgcttgggat tcaggcatcg cttcgcgttg gccgcctgtt cgatgaaggc aagatggcgc ccgaaatgat ttctatcgtc aagcgcaaca attgcggcaa ggctctggat attgcccgcc aggcccgtga tatgcatggc ggcaacggta tccagattga atatcacgtc atgcgccacg cgcagaatct ggaaaccgtc aacacctatg agggcacgca cgacgntcac gcgctgattc tcggccgtgc ccagaccggc attcaggcgt tcttctaatn a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSRAAFAWE DPFLLEEQLT EDERMIRDSA KAFASDVLLP RVEKAYLEET TDPELFHLMG QAGLLGVTLP EDYGAANASY VAYGLVAREV ERIDSGYRSM MSVQSSLVMY PIYAYGSDEQ RKKYLPGLVS GELIGCFGLT EPDAGSDPAG MKTRAEKIDG GYRLSGSKMW ISNSPIADVF VVWAKSAAHD NAIRGFILEK GMKGLSAPKI GGKLSLRASI TGEIVMDGVE VSEDAILPNV SGLKGPFGCL NRARYGISWG VLGAAEDCWF RARQYGLDRK QFNKPLAGTQ LYQKKLADMQ TEIALGIQAS LRVGRLFDEG KMAPEMISIV KRNNCGKALD IARQARDMHG GNGIQIEYHV MRHAQNLETV NTYEGTHDVH ALILGRAQTG IQAFF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcacgtgcg gcttttgcct gggaagatcc gtttttgctg gaggagcaac tgaccgaaga cgagcgcatg atccgcgact cggccaaggc ctttgcaagc gacgttcttc tgcctcgtgt cgaaaaagcc tatctggaag aaaccaccga cccggaactg tttcacctca tggggcaggc ggggcttctg ggcgtaacgc tgccggaaga ctatggcgcg gccaatgcaa gctatgtagc ctatgggctg gtggcgcgcg aagtggagcg cattgattcc ggatatcgtt ccatgatgag cgtgcaatcg tcgctcgtca tgtatccgat ctatgcctat ggttcggatg aacagcgcaa gaaatatctt cccggcctgg tttcgggtga attgattggc tgtttcggcc ttaccgaacc cgatgcgggt tccgacccgg ctggcatgaa aacccgcgcc gaaaagatcg atggcggcta tcgcctcagc ggctcgaaga tgtggatttc caattcaccg attgccgatg tgttcgtcgt ctgggcaaaa tcggctgcgc atgacaacgc cattcgcggc ttcattcttg aaaagggcat gaagggactt tcagcaccga agatcggggg caagctgtcg ctgcgcgctt ctatcaccgg cgagatcgtg atggatgggg ttgaagtttc cgaagacgca atcctgccga atgtttccgg tctcaagggc ccgtttggct gtctcaaccg cgcgcgctat ggcatttcgt ggggtgtgct cggggctgct gaagattgct ggttccgtgc gcgccaatat ggtctggacc gcaagcaatt caacaagccg ctggctggca cgcagcttta ccagaagaag ctggccgata tgcagacgga aatcgcgctt gggattcagg catcgcttcg cgttggccgc ctgttcgatg aaggcaagat ggcgcccgaa atgatttcta tcgtcaagcg caacaattgc ggcaaggctc tggatattgc ccgccaggcc cgtgatatgc atggcggcaa cggtatccag attgaatatc acgtcatgcg ccacgcgcag aatctggaaa ccgtcaacac ctatgagggc acgcacgacg ttcacgcgct gattctcggc cgtgcccaga ccggcattca ggcgttcttc tgaaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for BrabA.00027.a.D11.PD00015
PURIFICATION DATe: 6/4/2008
CONCENTRATION: 10.5mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 57
VIAL VOLUME: 50µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSMSR AAFAWEDPFL LEEQLTEDER MIRDSAKAFA SDVLLPRVEK AYLEETTDPE LFHLMGQAGL LGVTLPEDYG AANASYVAYG LVAREVERID SGYRSMMSVQ SSLVMYPIYA YGSDEQRKKY LPGLVSGELI GCFGLTEPDA GSDPAGMKTR AEKIDGGYRL SGSKMWISNS PIADVFVVWA KSAAHDNAIR GFILEKGMKG LSAPKIGGKL SLRASITGEI VMDGVEVSED AILPNVSGLK GPFGCLNRAR YGISWGVLGA AEDCWFRARQ YGLDRKQFNK PLAGTQLYQK KLADMQTEIA LGIQASLRVG RLFDEGKMAP EMISIVKRNN CGKALDIARQ ARDMHGGNGI QIEYHVMRHA QNLETVNTYE GTHDVHALIL GRAQTGIQAF F
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCCACC ACCACCACCA CCATAGCGGC GAAGTAAAAC CGGAAGTGAA GCCGGAGACC CACATCAACC TGAAGGTTAG CGACGGTAGC AGCGAAATCT TCTTCAAGAT TAAGAAGACC ACCCCGCTGC GTCGCCTGAT GGAAGCATTC GCTAAACGCC AGGGCAAGGA AATGGATTCC CTGCGCTTTC TGTACGACGG TATCCGTATT CAGGCAGACC AGACTCCGGA AGATCTGGAC ATGGAAGATA ACGACATTAT CGAAGCACAC CGTGAACAAA TCGGTGGATC CATGAGCCGT GCTGCCTTTG CCTGGGAGGA CCCATTTCTG CTGGAGGAGC AACTCACTGA AGATGAACGC ATGATCCGCG ACTCCGCTAA AGCCTTCGCA TCTGATGTGT TACTGCCGCG TGTTGAGAAA GCATACCTCG AAGAAACTAC CGATCCTGAA CTGTTTCATC TGATGGGTCA GGCCGGCCTG CTGGGCGTTA CTCTGCCTGA AGACTACGGT GCTGCCAACG CTTCTTACGT AGCTTACGGC CTCGTTGCTC GCGAAGTTGA ACGTATTGAT TCTGGCTACC GCTCCATGAT GTCTGTACAA TCGTCCCTCG TTATGTATCC GATCTACGCA TATGGTTCGG ACGAACAGCG TAAGAAATAC CTGCCTGGCC TGGTCTCTGG TGAACTGATT GGCTGCTTCG GCCTGACCGA ACCTGATGCT GGTTCTGATC CGGCTGGCAT GAAGACTCGT GCCGAGAAGA TTGACGGCGG CTACCGTCTG TCTGGTTCTA AGATGTGGAT CAGCAACTCT CCTATTGCCG ACGTCTTTGT AGTTTGGGCC AAATCCGCTG CACACGATAA CGCTATCCGT GGCTTTATCC TCGAGAAAGG CATGAAGGGC CTGAGCGCTC CTAAGATTGG CGGCAAGCTG TCCCTGCGTG CATCCATTAC CGGCGAGATC GTTATGGACG GTGTGGAGGT AAGCGAAGAC GCAATCCTGC CTAACGTTTC TGGTCTGAAA GGCCCGTTCG GCTGTCTGAA CCGTGCTCGT TACGGCATCT CCTGGGGTGT TCTGGGCGCA GCTGAAGACT GCTGGTTTCG CGCTCGTCAG TACGGCCTTG ACCGTAAGCA ATTCAACAAA CCGTTAGCTG GTACCCAGTT ATACCAGAAA AAACTGGCAG ACATGCAGAC CGAAATCGCT CTCGGCATTC AGGCTAGCCT GCGTGTAGGC CGTCTGTTCG ACGAAGGCAA AATGGCACCT GAGATGATCA GCATCGTTAA GCGTAACAAT TGCGGCAAGG CTCTTGACAT CGCTCGTCAA GCTCGTGACA TGCACGGCGG CAACGGTATC CAGATCGAAT ACCATGTTAT GCGTCACGCC CAGAACCTCG AAACCGTTAA CACCTACGAA GGTACTCACG ACGTGCACGC TCTCATCCTG GGTCGTGCTC AGACTGGTAT CCAGGCTTTC TTCTAAAAGC TTGCGGCCGC ACTCGAGCAC CACCACCACC ACCACTGAGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGAT
Details for BrabA.00027.a.A1.PS00026
PURIFICATION DATe: 6/19/2008
CONCENTRATION: 23.85mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 16
VIAL VOLUME: 100µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSRAAFAWE DPFLLEEQLT EDERMIRDSA KAFASDVLLP RVEKAYLEET TDPELFHLMG QAGLLGVTLP EDYGAANASY VAYGLVAREV ERIDSGYRSM MSVQSSLVMY PIYAYGSDEQ RKKYLPGLVS GELIGCFGLT EPDAGSDPAG MKTRAEKIDG GYRLSGSKMW ISNSPIADVF VVWAKSAAHD NAIRGFILEK GMKGLSAPKI GGKLSLRASI TGEIVMDGVE VSEDAILPNV SGLKGPFGCL NRARYGISWG VLGAAEDCWF RARQYGLXXK QFNKPLAGTQ LYQKKLADMQ TEIALGIQAS LRVGRLFDEG KMAPEMISIV KRNNCGKALD IARQARDMHG GNGIQIEYHV MRHAQNLETV NTYEGTHDXH ALILGRAQTG IQAFF
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtcac gtgcggcttt tgcctgggaa gatccgtttt tgctggagga gcaactgacc gaagacgagc gcatgatccg cgactcggcc aaggcctttg caagcgacgt tcttctgcct cgtgtcgaaa aagcctatct ggaagaaacc accgacccgg aactgtttca cctcatgggg caggcggggc ttctgggcgt aacgctgccg gaagactatg gcgcggccaa tgcaagctat gtagcctatg ggctggtggc gcgcgaagtg gagcgcattg attccggata tcgttccatg atgagcgtgc aatcgtcgct cgtcatgtat ccgatctatg cctatggttc ggatgaacag cgcaagaaat atcttcccgg cctggtttcg ggtgaattga ttggctgttt cggccttacc gaacccgatg cgggttccga cccggctggc atgaaaaccc gcgccgaaaa gatcgatggc ggctatcgcc tcagcggctc gaagatgtgg atttccaatt caccgattgc cgatgtgttc gtcgtctggg caaaatcggc tgcgcatgac aacgccattc gcggcttcat tcttgaaaag ggcatgaagg gactttcagc accgaagatc gggggcaagc tgtcgctgcg cgcttctatc accggcgaga tcgtgatgga tggggttgaa gtttccgaag acgcaatcct gccgaatgtt tccggtctca agggcccgtt tggctgtctc aaccgcgcgc gctatggcat ttcgtggggt gtgctcgggg ctgctgaaga ttgctggttc cgtgcgcgcc aatatggtct gganngcaag caattcaaca agccgctggc tggcacgcag ctttaccaga agaagctggc cgatatgcag acggaaatcg cgcttgggat tcaggcatcg cttcgcgttg gccgcctgtt cgatgaaggc aagatggcgc ccgaaatgat ttctatcgtc aagcgcaaca attgcggcaa ggctctggat attgcccgcc aggcccgtga tatgcatggc ggcaacggta tccagattga atatcacgtc atgcgccacg cgcagaatct ggaaaccgtc aacacctatg agggcacgca cgacgntcac gcgctgattc tcggccgtgc ccagaccggc attcaggcgt tcttctaatn a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSRAAFAWE DPFLLEEQLT EDERMIRDSA KAFASDVLLP RVEKAYLEET TDPELFHLMG QAGLLGVTLP EDYGAANASY VAYGLVAREV ERIDSGYRSM MSVQSSLVMY PIYAYGSDEQ RKKYLPGLVS GELIGCFGLT EPDAGSDPAG MKTRAEKIDG GYRLSGSKMW ISNSPIADVF VVWAKSAAHD NAIRGFILEK GMKGLSAPKI GGKLSLRASI TGEIVMDGVE VSEDAILPNV SGLKGPFGCL NRARYGISWG VLGAAEDCWF RARQYGLDRK QFNKPLAGTQ LYQKKLADMQ TEIALGIQAS LRVGRLFDEG KMAPEMISIV KRNNCGKALD IARQARDMHG GNGIQIEYHV MRHAQNLETV NTYEGTHDVH ALILGRAQTG IQAFF
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCACGTGCG GCTTTTGCCT GGGAAGATCC GTTTTTGCTG GAGGAGCAAC TGACCGAAGA CGAGCGCATG ATCCGCGACT CGGCCAAGGC CTTTGCAAGC GACGTTCTTC TGCCTCGTGT CGAAAAAGCC TATCTGGAAG AAACCACCGA CCCGGAACTG TTTCACCTCA TGGGGCAGGC GGGGCTTCTG GGCGTAACGC TGCCGGAAGA CTATGGCGCG GCCAATGCAA GCTATGTAGC CTATGGGCTG GTGGCGCGCG AAGTGGAGCG CATTGATTCC GGATATCGTT CCATGATGAG CGTGCAATCG TCGCTCGTCA TGTATCCGAT CTATGCCTAT GGTTCGGATG AACAGCGCAA GAAATATCTT CCCGGCCTGG TTTCGGGTGA ATTGATTGGC TGTTTCGGCC TTACCGAACC CGATGCGGGT TCCGACCCGG CTGGCATGAA AACCCGCGCC GAAAAGATCG ATGGCGGCTA TCGCCTCAGC GGCTCGAAGA TGTGGATTTC CAATTCACCG ATTGCCGATG TGTTCGTCGT CTGGGCAAAA TCGGCTGCGC ATGACAACGC CATTCGCGGC TTCATTCTTG AAAAGGGCAT GAAGGGACTT TCAGCACCGA AGATCGGGGG CAAGCTGTCG CTGCGCGCTT CTATCACCGG CGAGATCGTG ATGGATGGGG TTGAAGTTTC CGAAGACGCA ATCCTGCCGA ATGTTTCCGG TCTCAAGGGC CCGTTTGGCT GTCTCAACCG CGCGCGCTAT GGCATTTCGT GGGGTGTGCT CGGGGCTGCT GAAGATTGCT GGTTCCGTGC GCGCCAATAT GGTCTGGACC GCAAGCAATT CAACAAGCCG CTGGCTGGCA CGCAGCTTTA CCAGAAGAAG CTGGCCGATA TGCAGACGGA AATCGCGCTT GGGATTCAGG CATCGCTTCG CGTTGGCCGC CTGTTCGATG AAGGCAAGAT GGCGCCCGAA ATGATTTCTA TCGTCAAGCG CAACAATTGC GGCAAGGCTC TGGATATTGC CCGCCAGGCC CGTGATATGC ATGGCGGCAA CGGTATCCAG ATTGAATATC ACGTCATGCG CCACGCGCAG AATCTGGAAA CCGTCAACAC CTATGAGGGC ACGCACGACG TTCACGCGCT GATTCTCGGC CGTGCCCAGA CCGGCATTCA GGCGTTCTTC TGAAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA
Details for BrabA.00027.a.A1.PW26983
PURIFICATION DATe: 6/1/2009
CONCENTRATION: 29.14mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 29
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSRAAFAWE DPFLLEEQLT EDERMIRDSA KAFASDVLLP RVEKAYLEET TDPELFHLMG QAGLLGVTLP EDYGAANASY VAYGLVAREV ERIDSGYRSM MSVQSSLVMY PIYAYGSDEQ RKKYLPGLVS GELIGCFGLT EPDAGSDPAG MKTRAEKIDG GYRLSGSKMW ISNSPIADVF VVWAKSAAHD NAIRGFILEK GMKGLSAPKI GGKLSLRASI TGEIVMDGVE VSEDAILPNV SGLKGPFGCL NRARYGISWG VLGAAEDCWF RARQYGLXXK QFNKPLAGTQ LYQKKLADMQ TEIALGIQAS LRVGRLFDEG KMAPEMISIV KRNNCGKALD IARQARDMHG GNGIQIEYHV MRHAQNLETV NTYEGTHDXH ALILGRAQTG IQAFF
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtcac gtgcggcttt tgcctgggaa gatccgtttt tgctggagga gcaactgacc gaagacgagc gcatgatccg cgactcggcc aaggcctttg caagcgacgt tcttctgcct cgtgtcgaaa aagcctatct ggaagaaacc accgacccgg aactgtttca cctcatgggg caggcggggc ttctgggcgt aacgctgccg gaagactatg gcgcggccaa tgcaagctat gtagcctatg ggctggtggc gcgcgaagtg gagcgcattg attccggata tcgttccatg atgagcgtgc aatcgtcgct cgtcatgtat ccgatctatg cctatggttc ggatgaacag cgcaagaaat atcttcccgg cctggtttcg ggtgaattga ttggctgttt cggccttacc gaacccgatg cgggttccga cccggctggc atgaaaaccc gcgccgaaaa gatcgatggc ggctatcgcc tcagcggctc gaagatgtgg atttccaatt caccgattgc cgatgtgttc gtcgtctggg caaaatcggc tgcgcatgac aacgccattc gcggcttcat tcttgaaaag ggcatgaagg gactttcagc accgaagatc gggggcaagc tgtcgctgcg cgcttctatc accggcgaga tcgtgatgga tggggttgaa gtttccgaag acgcaatcct gccgaatgtt tccggtctca agggcccgtt tggctgtctc aaccgcgcgc gctatggcat ttcgtggggt gtgctcgggg ctgctgaaga ttgctggttc cgtgcgcgcc aatatggtct gganngcaag caattcaaca agccgctggc tggcacgcag ctttaccaga agaagctggc cgatatgcag acggaaatcg cgcttgggat tcaggcatcg cttcgcgttg gccgcctgtt cgatgaaggc aagatggcgc ccgaaatgat ttctatcgtc aagcgcaaca attgcggcaa ggctctggat attgcccgcc aggcccgtga tatgcatggc ggcaacggta tccagattga atatcacgtc atgcgccacg cgcagaatct ggaaaccgtc aacacctatg agggcacgca cgacgntcac gcgctgattc tcggccgtgc ccagaccggc attcaggcgt tcttctaatn a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSRAAFAWE DPFLLEEQLT EDERMIRDSA KAFASDVLLP RVEKAYLEET TDPELFHLMG QAGLLGVTLP EDYGAANASY VAYGLVAREV ERIDSGYRSM MSVQSSLVMY PIYAYGSDEQ RKKYLPGLVS GELIGCFGLT EPDAGSDPAG MKTRAEKIDG GYRLSGSKMW ISNSPIADVF VVWAKSAAHD NAIRGFILEK GMKGLSAPKI GGKLSLRASI TGEIVMDGVE VSEDAILPNV SGLKGPFGCL NRARYGISWG VLGAAEDCWF RARQYGLDRK QFNKPLAGTQ LYQKKLADMQ TEIALGIQAS LRVGRLFDEG KMAPEMISIV KRNNCGKALD IARQARDMHG GNGIQIEYHV MRHAQNLETV NTYEGTHDVH ALILGRAQTG IQAFF
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCACGTGCG GCTTTTGCCT GGGAAGATCC GTTTTTGCTG GAGGAGCAAC TGACCGAAGA CGAGCGCATG ATCCGCGACT CGGCCAAGGC CTTTGCAAGC GACGTTCTTC TGCCTCGTGT CGAAAAAGCC TATCTGGAAG AAACCACCGA CCCGGAACTG TTTCACCTCA TGGGGCAGGC GGGGCTTCTG GGCGTAACGC TGCCGGAAGA CTATGGCGCG GCCAATGCAA GCTATGTAGC CTATGGGCTG GTGGCGCGCG AAGTGGAGCG CATTGATTCC GGATATCGTT CCATGATGAG CGTGCAATCG TCGCTCGTCA TGTATCCGAT CTATGCCTAT GGTTCGGATG AACAGCGCAA GAAATATCTT CCCGGCCTGG TTTCGGGTGA ATTGATTGGC TGTTTCGGCC TTACCGAACC CGATGCGGGT TCCGACCCGG CTGGCATGAA AACCCGCGCC GAAAAGATCG ATGGCGGCTA TCGCCTCAGC GGCTCGAAGA TGTGGATTTC CAATTCACCG ATTGCCGATG TGTTCGTCGT CTGGGCAAAA TCGGCTGCGC ATGACAACGC CATTCGCGGC TTCATTCTTG AAAAGGGCAT GAAGGGACTT TCAGCACCGA AGATCGGGGG CAAGCTGTCG CTGCGCGCTT CTATCACCGG CGAGATCGTG ATGGATGGGG TTGAAGTTTC CGAAGACGCA ATCCTGCCGA ATGTTTCCGG TCTCAAGGGC CCGTTTGGCT GTCTCAACCG CGCGCGCTAT GGCATTTCGT GGGGTGTGCT CGGGGCTGCT GAAGATTGCT GGTTCCGTGC GCGCCAATAT GGTCTGGACC GCAAGCAATT CAACAAGCCG CTGGCTGGCA CGCAGCTTTA CCAGAAGAAG CTGGCCGATA TGCAGACGGA AATCGCGCTT GGGATTCAGG CATCGCTTCG CGTTGGCCGC CTGTTCGATG AAGGCAAGAT GGCGCCCGAA ATGATTTCTA TCGTCAAGCG CAACAATTGC GGCAAGGCTC TGGATATTGC CCGCCAGGCC CGTGATATGC ATGGCGGCAA CGGTATCCAG ATTGAATATC ACGTCATGCG CCACGCGCAG AATCTGGAAA CCGTCAACAC CTATGAGGGC ACGCACGACG TTCACGCGCT GATTCTCGGC CGTGCCCAGA CCGGCATTCA GGCGTTCTTC TGAAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA