BrabA.00023.a
Inorganic pyrophosphatase:Bacterial/Archaeal inorganic pyrophosphatase
| CENTER ID: | BrabA.00023.a |
| ORGANISM: | Brucella melitensis biovar Abortus 2308 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.00023.a.A1.GE25372 | Full length( BrabA.00023.a ) | 1 | 176 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
3FQ3
DEPOSITED: 1/6/2009
DETERMINATION: XRay
CLONE: BrabA.00023.a.A1.GE25372
PROTEIN: BrabA.00023.a.A1.PS00051
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|359391.4.peg.2076 |
| RefSeq: | YP_415326 |
| UniProt: | Q2YR76 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNIDAISIGS NPPEDVNVII EVPVGGQPIK YEMDKKAGAL IVDRFLYTPM TYPGNYGFVP HTLSEDGDPI DVLVCNTRPL IPGCVINVRP IGVLVMEDNS GKDEKIIAVP SPHLTRRYEK IHDYTDMPEI TLKQIAHFFE HYKDLEPGKW VKIGDWGDED YARKFIVEAI ERAKGK
NT Sequence
ATGAATATTG ATGCCATCTC CATCGGCTCC AATCCTCCTG AAGACGTCAA CGTAATCATT GAAGTGCCTG TCGGCGGCCA GCCGATCAAG TACGAGATGG ACAAGAAGGC CGGCGCGCTT ATCGTCGACC GTTTCCTTTA CACACCCATG ACCTATCCGG GCAATTACGG CTTCGTGCCG CATACGCTTT CGGAAGATGG CGATCCGATC GACGTTCTGG TCTGCAACAC GCGCCCGCTC ATTCCGGGTT GCGTGATCAA TGTGCGCCCG ATCGGCGTTC TGGTCATGGA AGACAATTCG GGCAAGGACG AGAAGATTAT CGCGGTGCCT TCGCCGCATC TTACGCGCCG CTATGAGAAG ATCCACGACT ATACCGATAT GCCGGAGATC ACGCTGAAGC AGATTGCGCA TTTCTTCGAG CACTACAAGG ATCTGGAACC CGGCAAGTGG GTCAAGATCG GCGACTGGGG CGATGAAGAC TATGCCCGCA AATTCATCGT GGAAGCTATC GAGCGCGCCA AGGGCAAG
Details for BrabA.00023.a.A1.GE25372
| HARVESTED ON: | 6/30/2008 |
| SEQUENCED ON: | 6/16/2009 |
| EXPECTED MW: | 22kDa |
| OBSERVED MW: | 23kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 95 |
Validated AA Sequence
MGTLEAQTQG PGSMNIDAIS IGSNPPEDVN VIIEVPVGGQ PIKYEMDKKA GALIVDRFLY TPMTYPGNYG FVPHTLSEDG DPIDVLVCNT RPLIPGCVIN VRPIGVLVME DNSGKDEKII AVPSPHLTRR YEKIHDYTDM PEITLKQIAH FFEHYKDLEP GKWVKIGDWG DEDYARKFIV EAIERAKGK
Validated NT Sequence
tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaatattgat gccatctcca tcggctccaa tcctcctgaa gacgtcaacg taatcattga agtgcctgtc ggcggccagc cgatcaagta cgagatggac aagaaggccg gcgcgcttat cgtcgaccgt ttcctttaca cacccatgac ctatccgggc aattacggct tcgtgccgca tacgctttcg gaagatggcg atccgatcga cgttctggtc tgcaacacgc gcccgctcat tccgggttgc gtgatcaatg tgcgcccgat cggcgttctg gtcatggaag acaattcggg caaggacgag aagattatcg cggtgccttc gccgcatctt acgcgccgct atgagaagat ccacgactat accgatatgc cggagatcac gctgaagcag attgcgcatt tcttcgagca ctacaaggat ctggaacccg gcaagtgggt caagatcggc gactggggcg atgaagacta tgcccgcaaa ttcatcgtgg aagctatcga gcgcgccaag ggcaagtaat aa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNIDAISIG SNPPEDVNVI IEVPVGGQPI KYEMDKKAGA LIVDRFLYTP MTYPGNYGFV PHTLSEDGDP IDVLVCNTRP LIPGCVINVR PIGVLVMEDN SGKDEKIIAV PSPHLTRRYE KIHDYTDMPE ITLKQIAHFF EHYKDLEPGK WVKIGDWGDE DYARKFIVEA IERAKGK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaatattgat gccatctcca tcggctccaa tcctcctgaa gacgtcaacg taatcattga agtgcctgtc ggcggccagc cgatcaagta cgagatggac aagaaggccg gcgcgcttat cgtcgaccgt ttcctttaca cacccatgac ctatccgggc aattacggct tcgtgccgca tacgctttcg gaagatggcg atccgatcga cgttctggtc tgcaacacgc gcccgctcat tccgggttgc gtgatcaatg tgcgcccgat cggcgttctg gtcatggaag acaattcggg caaggacgag aagattatcg cggtgccttc gccgcatctt acgcgccgct atgagaagat ccacgactat accgatatgc cggagatcac gctgaagcag attgcgcatt tcttcgagca ctacaaggat ctggaacccg gcaagtgggt caagatcggc gactggggcg atgaagacta tgcccgcaaa ttcatcgtgg aagctatcga gcgcgccaag ggcaagtaaa aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a