BrabA.00021.a

Amino acid-binding ACT:D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain:D-isomer specific 2-hydroxyacid dehydr...

CENTER ID: BrabA.00021.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: in PDB
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BrabA.00021.a.A1.GE25371 Full length( BrabA.00021.a ) 1 412
BrabA.00021.a.B1.GE25467 Full length( BrabA.00021.a ) 1 412
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BrabA.00021.a.B1.PS00091 Full length( BrabA.00021.a ) 1 412
BrabA.00021.a.A1.PW27469 Full length( BrabA.00021.a ) 1 412

Structures

3K5P
DEPOSITED: 10/7/2009
DETERMINATION: XRay
CLONE: BrabA.00021.a.A1.GE25371
PROTEIN: BrabA.00021.a.A1.PW27469

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_127006
PATRIC ID: fig|359391.4.peg.3078
RefSeq: YP_418952
UniProt: Q2YK82

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTERLSLSRD RINVLLLEGI SQTAVEYFKS SGYTNVTHLP KALDKADLIK AISSAHIIGI RSRTQLTEEI FAAANRLIAV GCFSVGTNQV ELKAARKRGI PVFNAPFSNT RSVAELVIGE IIMLMRRIFP RSVSAHAGGW EKTAIGSREV RGKTLGIVGY GNIGSQVGNL AESLGMTVRY YDTSDKLQYG NVKPAASLDE LLKTSDVVSL HVPSSKSTSK LITEAKLRKM KKGAFLINNA RGSVVDLEAL AKVLQEGHLA GAAIDVFPVE PASNGERFST PLQGLENVIL TPHIGGSTEE AQERIGTEVT RKLVEYSDVG STVGAVNFPQ VQLPPRPTGT RFMHVHENRP GILNSLMNVF SHHHINIASQ FLQTDGEVGY LVMEADGVGE ASDAVLQEIR EIPGTIRARL LY
NT Sequence
ATGACCGAAC GCCTCTCCCT CTCCCGTGAT CGCATCAATG TTCTTCTTCT GGAAGGCATC AGCCAGACCG CCGTGGAATA TTTCAAATCC TCCGGCTACA CCAATGTCAC ACATCTGCCG AAAGCATTGG ACAAGGCCGA TCTGATCAAG GCGATTTCTT CTGCGCATAT CATCGGCATT CGCTCGCGTA CGCAATTGAC CGAGGAGATT TTCGCCGCTG CCAACCGTCT GATCGCAGTC GGCTGCTTTT CGGTCGGCAC CAATCAGGTG GAACTGAAAG CTGCCCGCAA GCGCGGTATC CCCGTCTTCA ATGCGCCATT CTCCAACACG CGCTCGGTTG CCGAACTGGT GATTGGCGAG ATCATCATGC TGATGCGCCG CATTTTCCCG CGTTCCGTTT CAGCCCATGC GGGTGGCTGG GAAAAGACAG CCATCGGAAG CCGCGAAGTG CGCGGCAAGA CGCTCGGCAT CGTCGGCTAC GGCAATATCG GCTCGCAGGT CGGCAATCTG GCTGAAAGCC TCGGTATGAC CGTGCGTTAT TACGACACGT CCGACAAGCT GCAATATGGC AATGTGAAGC CTGCGGCGAG CCTTGATGAA CTTCTGAAAA CTTCCGATGT GGTGAGTCTG CACGTCCCGT CGAGCAAATC CACCTCGAAG CTCATCACCG AAGCCAAGCT GCGCAAGATG AAGAAGGGCG CTTTCCTCAT CAATAATGCG CGCGGTTCCG TTGTCGATCT CGAAGCCTTG GCCAAGGTTT TGCAGGAGGG TCATCTTGCG GGCGCGGCGA TCGATGTTTT CCCTGTGGAG CCGGCCTCCA ATGGCGAGAG GTTCTCAACC CCGCTTCAGG GGCTGGAGAA TGTCATTCTC ACGCCGCATA TCGGCGGCTC GACCGAGGAA GCGCAGGAAC GTATCGGTAC GGAAGTCACC CGCAAGCTGG TCGAATATTC CGATGTCGGC TCCACTGTGG GCGCCGTCAA TTTTCCGCAG GTGCAATTGC CGCCACGCCC GACCGGCACG CGCTTCATGC ATGTGCATGA AAACCGTCCG GGTATTCTGA ACAGCCTCAT GAACGTGTTT TCGCACCACC ATATCAATAT CGCCAGCCAG TTTCTCCAGA CGGATGGCGA GGTTGGCTAT CTCGTGATGG AAGCCGATGG CGTGGGCGAA GCAAGCGATG CCGTCTTGCA GGAAATCCGC GAAATTCCCG GCACGATTCG CGCTCGCCTG CTATAC
Details for BrabA.00021.a.A1.GE25371
HARVESTED ON: 6/30/2008
SEQUENCED ON: 6/16/2009
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 77
PERCENT COVERAGE: 84
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTERLSLSR DRINVLLLEG ISQTAVEYFK SSGYTNVTHL PKALDKADLI KAISSAHIIG IRSRTQLTEE IFAAANRLIA VGCFSVGTNQ VELKAARKRG IPVFNAPFSN TRSVAELVIG EIIMLMRRIF PRSVSAHAGG WEKTAIGSRE VRGKTLGIVG YGNIGSQVGN LAESLGMTVR YYDTSDKLQY GNVKPAASLD ELLKTSDVVS LHVPSSKSXS KLITEAKLRK MXXGAFLINN ARGSXVDLEA LXKVLXGGSS CGRGDXCFPC GAGXXXXEVL NPASGXGECX SHXXYRRLXX GXXXXYRXXS XXQXXXXXXX XXXXGRXQXX AXXIAXXXDX HXXXAXA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaccg aacgcctctc cctctcccgt gatcgcatca atgttcttct tctggaaggc atcagccaga ccgccgtgga atatttcaaa tcctccggct acaccaatgt cacacatctg ccgaaagcat tggacaaggc cgatctgatc aaggcgattt cttctgcgca tatcatcggc attcgctcgc gtacgcaatt gaccgaggag attttcgccg ctgccaaccg tctgatcgca gtcggctgct tttcggtcgg caccaatcag gtggaactga aagctgcccg caagcgcggt atccccgtct tcaatgcgcc attctccaac acgcgctcgg ttgccgaact ggtgattggc gagatcatca tgctgatgcg ccgcattttc ccgcgttccg tttcagccca tgcgggtggc tgggaaaaga cagccatcgg aagccgcgaa gtgcgcggca agacgctcgg catcgtcggc tacggcaata tcggctcgca ggtcggcaat ctggctgaaa gcctcggtat gaccgtgcgt tattacgaca cgtccgacaa gctgcaatat ggcaatgtga agcctgcggc gagccttgat gaacttctga aaacttccga tgtggtgagt ctgcacgtcc cgtcgagcaa atccncctcg aagctcatca ccgaagccaa gctgcgcaag atgaanaang gcgctttcct catcaataat gcgcgcggtt ccnatgtcga tctcgaagcc ttggncaagg ttttgcnagg agggtcatct tgcgggcgcg gcgatcnatg ttttccctgt ggagccggnn tncnnnggnn agaggttctc aaccccgctt cnggnnntgg agaatgtcnt tctcacgncn catatcggcg gctcgnnngn ggnagnncag nnnngtatcg gtanngaagt cancnncaan nngnnngaan nttnngnnnn nnnntncnct gngggcgccn ncaantnncn gcagnnncaa ttgccncnnc nnnngacnng cacgngnntc angcatgngc atgaaaaccg nnnnnntatt ctgnacagcc ncatgaacgn gttttcgcnn caccatnnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTERLSLSR DRINVLLLEG ISQTAVEYFK SSGYTNVTHL PKALDKADLI KAISSAHIIG IRSRTQLTEE IFAAANRLIA VGCFSVGTNQ VELKAARKRG IPVFNAPFSN TRSVAELVIG EIIMLMRRIF PRSVSAHAGG WEKTAIGSRE VRGKTLGIVG YGNIGSQVGN LAESLGMTVR YYDTSDKLQY GNVKPAASLD ELLKTSDVVS LHVPSSKSTS KLITEAKLRK MKKGAFLINN ARGSVVDLEA LAKVLQEGHL AGAAIDVFPV EPASNGERFS TPLQGLENVI LTPHIGGSTE EAQERIGTEV TRKLVEYSDV GSTVGAVNFP QVQLPPRPTG TRFMHVHENR PGILNSLMNV FSHHHINIAS QFLQTDGEVG YLVMEADGVG EASDAVLQEI REIPGTIRAR LLY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgaacgc ctctccctct cccgtgatcg catcaatgtt cttcttctgg aaggcatcag ccagaccgcc gtggaatatt tcaaatcctc cggctacacc aatgtcacac atctgccgaa agcattggac aaggccgatc tgatcaaggc gatttcttct gcgcatatca tcggcattcg ctcgcgtacg caattgaccg aggagatttt cgccgctgcc aaccgtctga tcgcagtcgg ctgcttttcg gtcggcacca atcaggtgga actgaaagct gcccgcaagc gcggtatccc cgtcttcaat gcgccattct ccaacacgcg ctcggttgcc gaactggtga ttggcgagat catcatgctg atgcgccgca ttttcccgcg ttccgtttca gcccatgcgg gtggctggga aaagacagcc atcggaagcc gcgaagtgcg cggcaagacg ctcggcatcg tcggctacgg caatatcggc tcgcaggtcg gcaatctggc tgaaagcctc ggtatgaccg tgcgttatta cgacacgtcc gacaagctgc aatatggcaa tgtgaagcct gcggcgagcc ttgatgaact tctgaaaact tccgatgtgg tgagtctgca cgtcccgtcg agcaaatcca cctcgaagct catcaccgaa gccaagctgc gcaagatgaa gaagggcgct ttcctcatca ataatgcgcg cggttccgtt gtcgatctcg aagccttggc caaggttttg caggagggtc atcttgcggg cgcggcgatc gatgttttcc ctgtggagcc ggcctccaat ggcgagaggt tctcaacccc gcttcagggg ctggagaatg tcattctcac gccgcatatc ggcggctcga ccgaggaagc gcaggaacgt atcggtacgg aagtcacccg caagctggtc gaatattccg atgtcggctc cactgtgggc gccgtcaatt ttccgcaggt gcaattgccg ccacgcccga ccggcacgcg cttcatgcat gtgcatgaaa accgtccggg tattctgaac agcctcatga acgtgttttc gcaccaccat atcaatatcg ccagccagtt tctccagacg gatggcgagg ttggctatct cgtgatggaa gccgatggcg tgggcgaagc aagcgatgcc gtcttgcagg aaatccgcga aattcccggc acgattcgcg ctcgcctgct atactgaaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for BrabA.00021.a.B1.GE25467
HARVESTED ON: 7/11/2008
SEQUENCED ON: 3/6/2009
EXPECTED MW: 45kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMT ERLSLSRDRI NVLLLEGISQ TAVEYFKSSG YTNVTHLPKA LDKADLIKAI SSAHIIGIRS RTQLTEEIFA AANRLIAVGC FSVGTNQVEL KAARKRGIPV FNAPFSNTRS VAELVIGEII MLMRRIFPRS VSAHAGGWEK TAIGSREVRG KTLGIVGYGN IGSQVGNLAE SLGMTVRYYD TSDKLQYGNV KPAASLDELL KTSDVVSLHV PSSKSTSKLI TEAKLRKMKK GAFLINNARG SDVDLEALAK VLQEGHLAGA AIDVFPVEPA SNGERFSTPL QGLENVILTP HIGGSTEEAQ ERIGTEVTRK LVEYSDVGST VGAVNFPQVQ LPPRPTGTRF MHVHENRPGI LNSLMNVFSH HHINIASQFL QTDGEVGYLV MEADGVGEAS DAVLQEIREI PGTIRARLLY
Validated NT Sequence
atggctcacc accaccacca ccatatgacc gaacgcctct ccctctcccg tgatcgcatc aatgttcttc ttctggaagg catcagccag accgccgtgg aatatttcaa atcctccggc tacaccaatg tcacacatct gccgaaagca ttggacaagg ccgatctgat caaggcgatt tcttctgcgc atatcatcgg cattcgctcg cgtacgcaat tgaccgagga gattttcgcc gctgccaacc gtctgatcgc agtcggctgc ttttcggtcg gcaccaatca ggtggaactg aaagctgccc gcaagcgcgg tatccccgtc ttcaatgcgc cattctccaa cacgcgctcg gttgccgaac tggtgattgg cgagatcatc atgctgatgc gccgcatttt cccgcgttcc gtttcagccc atgcgggtgg ctgggaaaag acagccatcg gaagccgcga agtgcgcggc aagacgctcg gcatcgtcgg ctacggcaat atcggctcgc aggtcggcaa tctggctgaa agcctcggta tgaccgtgcg ttattacgac acgtccgaca agctgcaata tggcaatgtg aagcctgcgg cgagccttga tgaacttctg aaaacttccg atgtggtgag tctgcacgtc ccgtcgagca aatccacctc gaagctcatc accgaagcca agctgcgcaa gatgaagaag ggcgctttcc tcatcaataa tgcgcgcggt tccgatgtcg atctcgaagc cttggccaag gttttgcagg agggtcatct tgcgggcgcg gcgatcgatg ttttccctgt ggagccggcc tccaatggcg agaggttctc aaccccgctt caggggctgg agaatgtcat tctcacgccg catatcggcg gctcgaccga ggaagcgcag gaacgtatcg gtacggaagt cacccgcaag ctggtcgaat attccgatgt cggctccact gtgggcgccg tcaattttcc gcaggtgcaa ttgccgccac gcccgaccgg cacgcgcttc atgcatgtgc atgaaaaccg tccgggtatt ctgaacagcc tcatgaacgt gttttcgcac caccatatca atatcgccag ccagtttctc cagacggatg gcgaggttgg ctatctcgtg atggaagccg atggcgtggg cgaagcaagc gatgccgtct tgcaggaaat ccgcgaaatt cccggcacga ttcgcgctcg cctgctatac taagtgagta agat
Expected Protein Sequence
MAHHHHHHMT ERLSLSRDRI NVLLLEGISQ TAVEYFKSSG YTNVTHLPKA LDKADLIKAI SSAHIIGIRS RTQLTEEIFA AANRLIAVGC FSVGTNQVEL KAARKRGIPV FNAPFSNTRS VAELVIGEII MLMRRIFPRS VSAHAGGWEK TAIGSREVRG KTLGIVGYGN IGSQVGNLAE SLGMTVRYYD TSDKLQYGNV KPAASLDELL KTSDVVSLHV PSSKSTSKLI TEAKLRKMKK GAFLINNARG SVVDLEALAK VLQEGHLAGA AIDVFPVEPA SNGERFSTPL QGLENVILTP HIGGSTEEAQ ERIGTEVTRK LVEYSDVGST VGAVNFPQVQ LPPRPTGTRF MHVHENRPGI LNSLMNVFSH HHINIASQFL QTDGEVGYLV MEADGVGEAS DAVLQEIREI PGTIRARLLY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccg aacgcctctc cctctcccgt gatcgcatca atgttcttct tctggaaggc atcagccaga ccgccgtgga atatttcaaa tcctccggct acaccaatgt cacacatctg ccgaaagcat tggacaaggc cgatctgatc aaggcgattt cttctgcgca tatcatcggc attcgctcgc gtacgcaatt gaccgaggag attttcgccg ctgccaaccg tctgatcgca gtcggctgct tttcggtcgg caccaatcag gtggaactga aagctgcccg caagcgcggt atccccgtct tcaatgcgcc attctccaac acgcgctcgg ttgccgaact ggtgattggc gagatcatca tgctgatgcg ccgcattttc ccgcgttccg tttcagccca tgcgggtggc tgggaaaaga cagccatcgg aagccgcgaa gtgcgcggca agacgctcgg catcgtcggc tacggcaata tcggctcgca ggtcggcaat ctggctgaaa gcctcggtat gaccgtgcgt tattacgaca cgtccgacaa gctgcaatat ggcaatgtga agcctgcggc gagccttgat gaacttctga aaacttccga tgtggtgagt ctgcacgtcc cgtcgagcaa atccacctcg aagctcatca ccgaagccaa gctgcgcaag atgaagaagg gcgctttcct catcaataat gcgcgcggtt ccgttgtcga tctcgaagcc ttggccaagg ttttgcagga gggtcatctt gcgggcgcgg cgatcgatgt tttccctgtg gagccggcct ccaatggcga gaggttctca accccgcttc aggggctgga gaatgtcatt ctcacgccgc atatcggcgg ctcgaccgag gaagcgcagg aacgtatcgg tacggaagtc acccgcaagc tggtcgaata ttccgatgtc ggctccactg tgggcgccgt caattttccg caggtgcaat tgccgccacg cccgaccggc acgcgcttca tgcatgtgca tgaaaaccgt ccgggtattc tgaacagcct catgaacgtg ttttcgcacc accatatcaa tatcgccagc cagtttctcc agacggatgg cgaggttggc tatctcgtga tggaagccga tggcgtgggc gaagcaagcg atgccgtctt gcaggaaatc cgcgaaattc ccggcacgat tcgcgctcgc ctgctatact gatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for BrabA.00021.a.B1.PS00091
PURIFICATION DATe: 10/21/2008
CONCENTRATION: 22.34mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 17
VIAL VOLUME: 100µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT ERLSLSRDRI NVLLLEGISQ TAVEYFKSSG YTNVTHLPKA LDKADLIKAI SSAHIIGIRS RTQLTEEIFA AANRLIAVGC FSVGTNQVEL KAARKRGIPV FNAPFSNTRS VAELVIGEII MLMRRIFPRS VSAHAGGWEK TAIGSREVRG KTLGIVGYGN IGSQVGNLAE SLGMTVRYYD TSDKLQYGNV KPAASLDELL KTSDVVSLHV PSSKSTSKLI TEAKLRKMKK GAFLINNARG SDVDLEALAK VLQEGHLAGA AIDVFPVEPA SNGERFSTPL QGLENVILTP HIGGSTEEAQ ERIGTEVTRK LVEYSDVGST VGAVNFPQVQ LPPRPTGTRF MHVHENRPGI LNSLMNVFSH HHINIASQFL QTDGEVGYLV MEADGVGEAS DAVLQEIREI PGTIRARLLY
Validated NT Sequence
atggctcacc accaccacca ccatatgacc gaacgcctct ccctctcccg tgatcgcatc aatgttcttc ttctggaagg catcagccag accgccgtgg aatatttcaa atcctccggc tacaccaatg tcacacatct gccgaaagca ttggacaagg ccgatctgat caaggcgatt tcttctgcgc atatcatcgg cattcgctcg cgtacgcaat tgaccgagga gattttcgcc gctgccaacc gtctgatcgc agtcggctgc ttttcggtcg gcaccaatca ggtggaactg aaagctgccc gcaagcgcgg tatccccgtc ttcaatgcgc cattctccaa cacgcgctcg gttgccgaac tggtgattgg cgagatcatc atgctgatgc gccgcatttt cccgcgttcc gtttcagccc atgcgggtgg ctgggaaaag acagccatcg gaagccgcga agtgcgcggc aagacgctcg gcatcgtcgg ctacggcaat atcggctcgc aggtcggcaa tctggctgaa agcctcggta tgaccgtgcg ttattacgac acgtccgaca agctgcaata tggcaatgtg aagcctgcgg cgagccttga tgaacttctg aaaacttccg atgtggtgag tctgcacgtc ccgtcgagca aatccacctc gaagctcatc accgaagcca agctgcgcaa gatgaagaag ggcgctttcc tcatcaataa tgcgcgcggt tccgatgtcg atctcgaagc cttggccaag gttttgcagg agggtcatct tgcgggcgcg gcgatcgatg ttttccctgt ggagccggcc tccaatggcg agaggttctc aaccccgctt caggggctgg agaatgtcat tctcacgccg catatcggcg gctcgaccga ggaagcgcag gaacgtatcg gtacggaagt cacccgcaag ctggtcgaat attccgatgt cggctccact gtgggcgccg tcaattttcc gcaggtgcaa ttgccgccac gcccgaccgg cacgcgcttc atgcatgtgc atgaaaaccg tccgggtatt ctgaacagcc tcatgaacgt gttttcgcac caccatatca atatcgccag ccagtttctc cagacggatg gcgaggttgg ctatctcgtg atggaagccg atggcgtggg cgaagcaagc gatgccgtct tgcaggaaat ccgcgaaatt cccggcacga ttcgcgctcg cctgctatac taagtgagta agat
Expressed Protein Sequence
MAHHHHHHMT ERLSLSRDRI NVLLLEGISQ TAVEYFKSSG YTNVTHLPKA LDKADLIKAI SSAHIIGIRS RTQLTEEIFA AANRLIAVGC FSVGTNQVEL KAARKRGIPV FNAPFSNTRS VAELVIGEII MLMRRIFPRS VSAHAGGWEK TAIGSREVRG KTLGIVGYGN IGSQVGNLAE SLGMTVRYYD TSDKLQYGNV KPAASLDELL KTSDVVSLHV PSSKSTSKLI TEAKLRKMKK GAFLINNARG SVVDLEALAK VLQEGHLAGA AIDVFPVEPA SNGERFSTPL QGLENVILTP HIGGSTEEAQ ERIGTEVTRK LVEYSDVGST VGAVNFPQVQ LPPRPTGTRF MHVHENRPGI LNSLMNVFSH HHINIASQFL QTDGEVGYLV MEADGVGEAS DAVLQEIREI PGTIRARLLY
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACCG AACGCCTCTC CCTCTCCCGT GATCGCATCA ATGTTCTTCT TCTGGAAGGC ATCAGCCAGA CCGCCGTGGA ATATTTCAAA TCCTCCGGCT ACACCAATGT CACACATCTG CCGAAAGCAT TGGACAAGGC CGATCTGATC AAGGCGATTT CTTCTGCGCA TATCATCGGC ATTCGCTCGC GTACGCAATT GACCGAGGAG ATTTTCGCCG CTGCCAACCG TCTGATCGCA GTCGGCTGCT TTTCGGTCGG CACCAATCAG GTGGAACTGA AAGCTGCCCG CAAGCGCGGT ATCCCCGTCT TCAATGCGCC ATTCTCCAAC ACGCGCTCGG TTGCCGAACT GGTGATTGGC GAGATCATCA TGCTGATGCG CCGCATTTTC CCGCGTTCCG TTTCAGCCCA TGCGGGTGGC TGGGAAAAGA CAGCCATCGG AAGCCGCGAA GTGCGCGGCA AGACGCTCGG CATCGTCGGC TACGGCAATA TCGGCTCGCA GGTCGGCAAT CTGGCTGAAA GCCTCGGTAT GACCGTGCGT TATTACGACA CGTCCGACAA GCTGCAATAT GGCAATGTGA AGCCTGCGGC GAGCCTTGAT GAACTTCTGA AAACTTCCGA TGTGGTGAGT CTGCACGTCC CGTCGAGCAA ATCCACCTCG AAGCTCATCA CCGAAGCCAA GCTGCGCAAG ATGAAGAAGG GCGCTTTCCT CATCAATAAT GCGCGCGGTT CCGTTGTCGA TCTCGAAGCC TTGGCCAAGG TTTTGCAGGA GGGTCATCTT GCGGGCGCGG CGATCGATGT TTTCCCTGTG GAGCCGGCCT CCAATGGCGA GAGGTTCTCA ACCCCGCTTC AGGGGCTGGA GAATGTCATT CTCACGCCGC ATATCGGCGG CTCGACCGAG GAAGCGCAGG AACGTATCGG TACGGAAGTC ACCCGCAAGC TGGTCGAATA TTCCGATGTC GGCTCCACTG TGGGCGCCGT CAATTTTCCG CAGGTGCAAT TGCCGCCACG CCCGACCGGC ACGCGCTTCA TGCATGTGCA TGAAAACCGT CCGGGTATTC TGAACAGCCT CATGAACGTG TTTTCGCACC ACCATATCAA TATCGCCAGC CAGTTTCTCC AGACGGATGG CGAGGTTGGC TATCTCGTGA TGGAAGCCGA TGGCGTGGGC GAAGCAAGCG ATGCCGTCTT GCAGGAAATC CGCGAAATTC CCGGCACGAT TCGCGCTCGC CTGCTATACT GATGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT
Details for BrabA.00021.a.A1.PW27469
PURIFICATION DATe: 7/14/2009
CONCENTRATION: 27.99mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 13
VIAL VOLUME: 200µl
PERCENT IDENTITY: 77
PERCENT COVERAGE: 84
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTERLSLSR DRINVLLLEG ISQTAVEYFK SSGYTNVTHL PKALDKADLI KAISSAHIIG IRSRTQLTEE IFAAANRLIA VGCFSVGTNQ VELKAARKRG IPVFNAPFSN TRSVAELVIG EIIMLMRRIF PRSVSAHAGG WEKTAIGSRE VRGKTLGIVG YGNIGSQVGN LAESLGMTVR YYDTSDKLQY GNVKPAASLD ELLKTSDVVS LHVPSSKSXS KLITEAKLRK MXXGAFLINN ARGSXVDLEA LXKVLXGGSS CGRGDXCFPC GAGXXXXEVL NPASGXGECX SHXXYRRLXX GXXXXYRXXS XXQXXXXXXX XXXXGRXQXX AXXIAXXXDX HXXXAXA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaccg aacgcctctc cctctcccgt gatcgcatca atgttcttct tctggaaggc atcagccaga ccgccgtgga atatttcaaa tcctccggct acaccaatgt cacacatctg ccgaaagcat tggacaaggc cgatctgatc aaggcgattt cttctgcgca tatcatcggc attcgctcgc gtacgcaatt gaccgaggag attttcgccg ctgccaaccg tctgatcgca gtcggctgct tttcggtcgg caccaatcag gtggaactga aagctgcccg caagcgcggt atccccgtct tcaatgcgcc attctccaac acgcgctcgg ttgccgaact ggtgattggc gagatcatca tgctgatgcg ccgcattttc ccgcgttccg tttcagccca tgcgggtggc tgggaaaaga cagccatcgg aagccgcgaa gtgcgcggca agacgctcgg catcgtcggc tacggcaata tcggctcgca ggtcggcaat ctggctgaaa gcctcggtat gaccgtgcgt tattacgaca cgtccgacaa gctgcaatat ggcaatgtga agcctgcggc gagccttgat gaacttctga aaacttccga tgtggtgagt ctgcacgtcc cgtcgagcaa atccncctcg aagctcatca ccgaagccaa gctgcgcaag atgaanaang gcgctttcct catcaataat gcgcgcggtt ccnatgtcga tctcgaagcc ttggncaagg ttttgcnagg agggtcatct tgcgggcgcg gcgatcnatg ttttccctgt ggagccggnn tncnnnggnn agaggttctc aaccccgctt cnggnnntgg agaatgtcnt tctcacgncn catatcggcg gctcgnnngn ggnagnncag nnnngtatcg gtanngaagt cancnncaan nngnnngaan nttnngnnnn nnnntncnct gngggcgccn ncaantnncn gcagnnncaa ttgccncnnc nnnngacnng cacgngnntc angcatgngc atgaaaaccg nnnnnntatt ctgnacagcc ncatgaacgn gttttcgcnn caccatnnna
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTERLSLSR DRINVLLLEG ISQTAVEYFK SSGYTNVTHL PKALDKADLI KAISSAHIIG IRSRTQLTEE IFAAANRLIA VGCFSVGTNQ VELKAARKRG IPVFNAPFSN TRSVAELVIG EIIMLMRRIF PRSVSAHAGG WEKTAIGSRE VRGKTLGIVG YGNIGSQVGN LAESLGMTVR YYDTSDKLQY GNVKPAASLD ELLKTSDVVS LHVPSSKSTS KLITEAKLRK MKKGAFLINN ARGSVVDLEA LAKVLQEGHL AGAAIDVFPV EPASNGERFS TPLQGLENVI LTPHIGGSTE EAQERIGTEV TRKLVEYSDV GSTVGAVNFP QVQLPPRPTG TRFMHVHENR PGILNSLMNV FSHHHINIAS QFLQTDGEVG YLVMEADGVG EASDAVLQEI REIPGTIRAR LLY
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCGAACGC CTCTCCCTCT CCCGTGATCG CATCAATGTT CTTCTTCTGG AAGGCATCAG CCAGACCGCC GTGGAATATT TCAAATCCTC CGGCTACACC AATGTCACAC ATCTGCCGAA AGCATTGGAC AAGGCCGATC TGATCAAGGC GATTTCTTCT GCGCATATCA TCGGCATTCG CTCGCGTACG CAATTGACCG AGGAGATTTT CGCCGCTGCC AACCGTCTGA TCGCAGTCGG CTGCTTTTCG GTCGGCACCA ATCAGGTGGA ACTGAAAGCT GCCCGCAAGC GCGGTATCCC CGTCTTCAAT GCGCCATTCT CCAACACGCG CTCGGTTGCC GAACTGGTGA TTGGCGAGAT CATCATGCTG ATGCGCCGCA TTTTCCCGCG TTCCGTTTCA GCCCATGCGG GTGGCTGGGA AAAGACAGCC ATCGGAAGCC GCGAAGTGCG CGGCAAGACG CTCGGCATCG TCGGCTACGG CAATATCGGC TCGCAGGTCG GCAATCTGGC TGAAAGCCTC GGTATGACCG TGCGTTATTA CGACACGTCC GACAAGCTGC AATATGGCAA TGTGAAGCCT GCGGCGAGCC TTGATGAACT TCTGAAAACT TCCGATGTGG TGAGTCTGCA CGTCCCGTCG AGCAAATCCA CCTCGAAGCT CATCACCGAA GCCAAGCTGC GCAAGATGAA GAAGGGCGCT TTCCTCATCA ATAATGCGCG CGGTTCCGTT GTCGATCTCG AAGCCTTGGC CAAGGTTTTG CAGGAGGGTC ATCTTGCGGG CGCGGCGATC GATGTTTTCC CTGTGGAGCC GGCCTCCAAT GGCGAGAGGT TCTCAACCCC GCTTCAGGGG CTGGAGAATG TCATTCTCAC GCCGCATATC GGCGGCTCGA CCGAGGAAGC GCAGGAACGT ATCGGTACGG AAGTCACCCG CAAGCTGGTC GAATATTCCG ATGTCGGCTC CACTGTGGGC GCCGTCAATT TTCCGCAGGT GCAATTGCCG CCACGCCCGA CCGGCACGCG CTTCATGCAT GTGCATGAAA ACCGTCCGGG TATTCTGAAC AGCCTCATGA ACGTGTTTTC GCACCACCAT ATCAATATCG CCAGCCAGTT TCTCCAGACG GATGGCGAGG TTGGCTATCT CGTGATGGAA GCCGATGGCG TGGGCGAAGC AAGCGATGCC GTCTTGCAGG AAATCCGCGA AATTCCCGGC ACGATTCGCG CTCGCCTGCT ATACTGAAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA