BrabA.00020.a
Aldehyde dehydrogenase
| CENTER ID: | BrabA.00020.a |
| ORGANISM: | Brucella melitensis biovar Abortus 2308 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.00020.a.A1.GE25370 | Full length( BrabA.00020.a ) | 1 | 483 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.00020.a.A1.PS00050 | Full length( BrabA.00020.a ) | 1 | 483 |
Structures
3EK1
DEPOSITED: 9/18/2008
DETERMINATION: XRay
CLONE: BrabA.00020.a.A1.GE25370
PROTEIN: BrabA.00020.a.A1.PS00050
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127004 |
| PATRIC ID: | fig|359391.4.peg.1719 |
| RefSeq: | YP_415006 |
| UniProt: | Q2YRI3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLALKDPSLL KSQCLVNGRW IDAADGTTIK VTNPADGSVI GTVPSLSVAT IKEAIDASAK ALSGWAAKTA KERAGILRKW FDLIIANADD IALIMTSEQG KPLAEARGEV LYAASFIEWF AEEAKRVYGD TIPAPQNGQR LTVIRQPVGV TAAITPWNFP AAMITRKAAP ALAAGCTMIV RPADLTPLTA LALGVLAEKA GIPAGVLQIV TGKAREIGAE LTSNDTVRKL SFTGSTEVGR LLMAQCAPTI KRISLELGGN APFIVFDDAD LDAAVDGAMV SKYRNAGQTC VCANRIYVQR GVYDKFAEKL AAKVKELKVG NGTEPGVVIG PMIEEKAITK VKAHIEDAVS KGAKLITGGK ELGGLFFEPG ILTGVTSDML VAKEETFGPL APLFAFDTEE EVIAQANDTI FGLAAYFYTE NFSRAIRVSE ALEYGMVGHN TGLISNEVAP FGGVKQSGLG REGSKYGIEE YLETKYICSA YKR
NT Sequence
ATGCTTGCTT TGAAAGACCC TTCGCTCCTT AAATCGCAAT GCCTCGTGAA CGGTCGCTGG ATCGACGCCG CAGATGGCAC GACGATCAAA GTGACAAACC CGGCTGACGG CTCCGTCATC GGCACGGTTC CCTCCCTGTC TGTTGCTACG ATCAAGGAAG CCATCGACGC CTCTGCCAAG GCGCTTTCCG GTTGGGCGGC GAAAACAGCC AAGGAACGCG CGGGCATTCT GCGCAAATGG TTCGACCTTA TCATCGCCAA TGCCGACGAT ATCGCTCTTA TAATGACGTC GGAGCAGGGA AAACCGCTTG CGGAAGCCCG CGGCGAGGTT CTTTATGCGG CATCCTTCAT TGAGTGGTTC GCGGAAGAAG CAAAGCGTGT CTATGGCGAC ACGATCCCTG CACCGCAGAA CGGCCAGCGC CTGACCGTCA TCCGCCAGCC TGTCGGTGTC ACGGCGGCGA TTACGCCGTG GAATTTCCCG GCGGCAATGA TTACCCGCAA GGCTGCTCCG GCGCTTGCAG CAGGCTGCAC CATGATCGTG CGCCCCGCCG ACCTCACCCC GCTGACCGCA CTGGCGCTTG GTGTTCTGGC CGAAAAGGCA GGCATTCCGG CTGGCGTGTT GCAGATCGTG ACTGGCAAGG CCCGCGAAAT CGGCGCGGAA CTCACCAGCA ATGACACCGT GCGCAAACTT TCCTTCACCG GATCGACGGA AGTGGGGCGC CTTCTGATGG CGCAGTGCGC CCCGACCATC AAGCGCATCT CGCTGGAGCT TGGCGGCAAC GCGCCCTTCA TCGTGTTCGA CGATGCCGAT CTTGATGCCG CCGTGGATGG CGCGATGGTT TCCAAATATC GCAATGCGGG CCAGACCTGC GTTTGCGCCA ACCGCATTTA TGTACAGCGC GGCGTTTACG ACAAGTTCGC CGAAAAGCTT GCGGCCAAGG TCAAGGAACT GAAGGTCGGC AACGGCACGG AACCCGGCGT CGTCATCGGC CCGATGATCG AAGAAAAAGC CATCACCAAG GTGAAGGCGC ATATTGAAGA CGCGGTTTCC AAGGGCGCAA AGCTCATCAC AGGCGGCAAG GAACTCGGCG GCCTGTTCTT CGAGCCGGGC ATCCTGACCG GCGTGACCTC CGATATGCTG GTCGCCAAGG AAGAAACCTT CGGCCCACTC GCTCCGCTTT TTGCCTTCGA CACGGAAGAA GAAGTCATTG CCCAGGCCAA TGACACGATC TTCGGTCTTG CCGCTTATTT CTATACGGAA AATTTCAGCC GTGCGATCCG GGTCAGCGAA GCGCTGGAAT ATGGCATGGT CGGCCACAAT ACCGGCCTCA TCTCCAATGA AGTCGCACCC TTTGGCGGCG TAAAGCAGTC TGGCCTCGGC CGCGAAGGCT CCAAATATGG TATCGAGGAA TATCTCGAAA CCAAATATAT CTGTAGCGCC TATAAGCGG
Details for BrabA.00020.a.A1.GE25370
| HARVESTED ON: | 6/30/2008 |
| SEQUENCED ON: | 6/16/2009 |
| EXPECTED MW: | 53kDa |
| OBSERVED MW: | 53kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 52 |
Validated AA Sequence
MGTLEAQTQG PGSMLALKDP SLLKSQCLVN GRWIDAADGT TIKVTNPADG SVIGTVPSLS VATIKEAIDA SAKALSGWAA KTAKERAGIL RKWFDLIIAN ADDIALIMTS EQGKPLAEAR GEVLYAASFI EWFAEEAKRV YGDTIPAPQN GQRLTVIRQP VGVTAAITPW NFPAAMITRK AAPALAAGCT MIVRPADLTP LTALALGVLA XKAGIPAGVL QIVTGKAREI GAELTSNDTV RKLSFTGSTE VGRLLMAQCA PTIKRIS
Validated NT Sequence
gctnatcacc atncaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgcttgct ttgaaagacc cttcgctcct taaatcgcaa tgcctcgtga acggtcgctg gatcgacgcc gcagatggca cgacgatcaa agtgacaaac ccggctgacg gctccgtcat cggcacggtt ccctccctgt ctgttgctac gatcaaggaa gccatcgacg cctctgccaa ggcgctttcc ggttgggcgg cgaaaacagc caaggaacgc gcgggcattc tgcgcaaatg gttcgacctt atcatcgcca atgccgacga tatcgctctt ataatgacgt cggagcaggg aaaaccgctt gcggaagccc gcggcgaggt tctttatgcg gcatccttca ttgagtggtt cgcggaagaa gcaaagcgtg tctatggcga cacgatccct gcaccgcaga acggccagcg cctgaccgtc atccgccagc ctgtcggtgt cacggcggcg attacgccgt ggaatttccc ggcggcaatg attacccgca aggctgctcc ggcgcttgca gcaggctgca ccatgatcgt gcgccccgcc gacctcaccc cgctgaccgc actggcgctt ggtgttctgg ccganaaggc aggcattccg gctggcgtgt tgcagatcgt gactggcaag gcccgcgaaa tcggcgcgga actcaccagc aatgacaccg tgcgcaaact ttccttcacc ggatcgacng aagtggggcg ncttctgatg gcgcagtgcg ccccgaccat caagcgcatc tcgc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLALKDPSL LKSQCLVNGR WIDAADGTTI KVTNPADGSV IGTVPSLSVA TIKEAIDASA KALSGWAAKT AKERAGILRK WFDLIIANAD DIALIMTSEQ GKPLAEARGE VLYAASFIEW FAEEAKRVYG DTIPAPQNGQ RLTVIRQPVG VTAAITPWNF PAAMITRKAA PALAAGCTMI VRPADLTPLT ALALGVLAEK AGIPAGVLQI VTGKAREIGA ELTSNDTVRK LSFTGSTEVG RLLMAQCAPT IKRISLELGG NAPFIVFDDA DLDAAVDGAM VSKYRNAGQT CVCANRIYVQ RGVYDKFAEK LAAKVKELKV GNGTEPGVVI GPMIEEKAIT KVKAHIEDAV SKGAKLITGG KELGGLFFEP GILTGVTSDM LVAKEETFGP LAPLFAFDTE EEVIAQANDT IFGLAAYFYT ENFSRAIRVS EALEYGMVGH NTGLISNEVA PFGGVKQSGL GREGSKYGIE EYLETKYICS AYKR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcttgctttg aaagaccctt cgctccttaa atcgcaatgc ctcgtgaacg gtcgctggat cgacgccgca gatggcacga cgatcaaagt gacaaacccg gctgacggct ccgtcatcgg cacggttccc tccctgtctg ttgctacgat caaggaagcc atcgacgcct ctgccaaggc gctttccggt tgggcggcga aaacagccaa ggaacgcgcg ggcattctgc gcaaatggtt cgaccttatc atcgccaatg ccgacgatat cgctcttata atgacgtcgg agcagggaaa accgcttgcg gaagcccgcg gcgaggttct ttatgcggca tccttcattg agtggttcgc ggaagaagca aagcgtgtct atggcgacac gatccctgca ccgcagaacg gccagcgcct gaccgtcatc cgccagcctg tcggtgtcac ggcggcgatt acgccgtgga atttcccggc ggcaatgatt acccgcaagg ctgctccggc gcttgcagca ggctgcacca tgatcgtgcg ccccgccgac ctcaccccgc tgaccgcact ggcgcttggt gttctggccg aaaaggcagg cattccggct ggcgtgttgc agatcgtgac tggcaaggcc cgcgaaatcg gcgcggaact caccagcaat gacaccgtgc gcaaactttc cttcaccgga tcgacggaag tggggcgcct tctgatggcg cagtgcgccc cgaccatcaa gcgcatctcg ctggagcttg gcggcaacgc gcccttcatc gtgttcgacg atgccgatct tgatgccgcc gtggatggcg cgatggtttc caaatatcgc aatgcgggcc agacctgcgt ttgcgccaac cgcatttatg tacagcgcgg cgtttacgac aagttcgccg aaaagcttgc ggccaaggtc aaggaactga aggtcggcaa cggcacggaa cccggcgtcg tcatcggccc gatgatcgaa gaaaaagcca tcaccaaggt gaaggcgcat attgaagacg cggtttccaa gggcgcaaag ctcatcacag gcggcaagga actcggcggc ctgttcttcg agccgggcat cctgaccggc gtgacctccg atatgctggt cgccaaggaa gaaaccttcg gcccactcgc tccgcttttt gccttcgaca cggaagaaga agtcattgcc caggccaatg acacgatctt cggtcttgcc gcttatttct atacggaaaa tttcagccgt gcgatccggg tcagcgaagc gctggaatat ggcatggtcg gccacaatac cggcctcatc tccaatgaag tcgcaccctt tggcggcgta aagcagtctg gcctcggccg cgaaggctcc aaatatggta tcgaggaata tctcgaaacc aaatatatct gtagcgccta taagcggtga aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for BrabA.00020.a.A1.PS00050
| PURIFICATION DATe: | 7/30/2008 |
| CONCENTRATION: | 34.7mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 52 |
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMLALKDP SLLKSQCLVN GRWIDAADGT TIKVTNPADG SVIGTVPSLS VATIKEAIDA SAKALSGWAA KTAKERAGIL RKWFDLIIAN ADDIALIMTS EQGKPLAEAR GEVLYAASFI EWFAEEAKRV YGDTIPAPQN GQRLTVIRQP VGVTAAITPW NFPAAMITRK AAPALAAGCT MIVRPADLTP LTALALGVLA XKAGIPAGVL QIVTGKAREI GAELTSNDTV RKLSFTGSTE VGRLLMAQCA PTIKRIS
Validated NT Sequence
gctnatcacc atncaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgcttgct ttgaaagacc cttcgctcct taaatcgcaa tgcctcgtga acggtcgctg gatcgacgcc gcagatggca cgacgatcaa agtgacaaac ccggctgacg gctccgtcat cggcacggtt ccctccctgt ctgttgctac gatcaaggaa gccatcgacg cctctgccaa ggcgctttcc ggttgggcgg cgaaaacagc caaggaacgc gcgggcattc tgcgcaaatg gttcgacctt atcatcgcca atgccgacga tatcgctctt ataatgacgt cggagcaggg aaaaccgctt gcggaagccc gcggcgaggt tctttatgcg gcatccttca ttgagtggtt cgcggaagaa gcaaagcgtg tctatggcga cacgatccct gcaccgcaga acggccagcg cctgaccgtc atccgccagc ctgtcggtgt cacggcggcg attacgccgt ggaatttccc ggcggcaatg attacccgca aggctgctcc ggcgcttgca gcaggctgca ccatgatcgt gcgccccgcc gacctcaccc cgctgaccgc actggcgctt ggtgttctgg ccganaaggc aggcattccg gctggcgtgt tgcagatcgt gactggcaag gcccgcgaaa tcggcgcgga actcaccagc aatgacaccg tgcgcaaact ttccttcacc ggatcgacng aagtggggcg ncttctgatg gcgcagtgcg ccccgaccat caagcgcatc tcgc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLALKDPSL LKSQCLVNGR WIDAADGTTI KVTNPADGSV IGTVPSLSVA TIKEAIDASA KALSGWAAKT AKERAGILRK WFDLIIANAD DIALIMTSEQ GKPLAEARGE VLYAASFIEW FAEEAKRVYG DTIPAPQNGQ RLTVIRQPVG VTAAITPWNF PAAMITRKAA PALAAGCTMI VRPADLTPLT ALALGVLAEK AGIPAGVLQI VTGKAREIGA ELTSNDTVRK LSFTGSTEVG RLLMAQCAPT IKRISLELGG NAPFIVFDDA DLDAAVDGAM VSKYRNAGQT CVCANRIYVQ RGVYDKFAEK LAAKVKELKV GNGTEPGVVI GPMIEEKAIT KVKAHIEDAV SKGAKLITGG KELGGLFFEP GILTGVTSDM LVAKEETFGP LAPLFAFDTE EEVIAQANDT IFGLAAYFYT ENFSRAIRVS EALEYGMVGH NTGLISNEVA PFGGVKQSGL GREGSKYGIE EYLETKYICS AYKR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCTTGCTTTG AAAGACCCTT CGCTCCTTAA ATCGCAATGC CTCGTGAACG GTCGCTGGAT CGACGCCGCA GATGGCACGA CGATCAAAGT GACAAACCCG GCTGACGGCT CCGTCATCGG CACGGTTCCC TCCCTGTCTG TTGCTACGAT CAAGGAAGCC ATCGACGCCT CTGCCAAGGC GCTTTCCGGT TGGGCGGCGA AAACAGCCAA GGAACGCGCG GGCATTCTGC GCAAATGGTT CGACCTTATC ATCGCCAATG CCGACGATAT CGCTCTTATA ATGACGTCGG AGCAGGGAAA ACCGCTTGCG GAAGCCCGCG GCGAGGTTCT TTATGCGGCA TCCTTCATTG AGTGGTTCGC GGAAGAAGCA AAGCGTGTCT ATGGCGACAC GATCCCTGCA CCGCAGAACG GCCAGCGCCT GACCGTCATC CGCCAGCCTG TCGGTGTCAC GGCGGCGATT ACGCCGTGGA ATTTCCCGGC GGCAATGATT ACCCGCAAGG CTGCTCCGGC GCTTGCAGCA GGCTGCACCA TGATCGTGCG CCCCGCCGAC CTCACCCCGC TGACCGCACT GGCGCTTGGT GTTCTGGCCG AAAAGGCAGG CATTCCGGCT GGCGTGTTGC AGATCGTGAC TGGCAAGGCC CGCGAAATCG GCGCGGAACT CACCAGCAAT GACACCGTGC GCAAACTTTC CTTCACCGGA TCGACGGAAG TGGGGCGCCT TCTGATGGCG CAGTGCGCCC CGACCATCAA GCGCATCTCG CTGGAGCTTG GCGGCAACGC GCCCTTCATC GTGTTCGACG ATGCCGATCT TGATGCCGCC GTGGATGGCG CGATGGTTTC CAAATATCGC AATGCGGGCC AGACCTGCGT TTGCGCCAAC CGCATTTATG TACAGCGCGG CGTTTACGAC AAGTTCGCCG AAAAGCTTGC GGCCAAGGTC AAGGAACTGA AGGTCGGCAA CGGCACGGAA CCCGGCGTCG TCATCGGCCC GATGATCGAA GAAAAAGCCA TCACCAAGGT GAAGGCGCAT ATTGAAGACG CGGTTTCCAA GGGCGCAAAG CTCATCACAG GCGGCAAGGA ACTCGGCGGC CTGTTCTTCG AGCCGGGCAT CCTGACCGGC GTGACCTCCG ATATGCTGGT CGCCAAGGAA GAAACCTTCG GCCCACTCGC TCCGCTTTTT GCCTTCGACA CGGAAGAAGA AGTCATTGCC CAGGCCAATG ACACGATCTT CGGTCTTGCC GCTTATTTCT ATACGGAAAA TTTCAGCCGT GCGATCCGGG TCAGCGAAGC GCTGGAATAT GGCATGGTCG GCCACAATAC CGGCCTCATC TCCAATGAAG TCGCACCCTT TGGCGGCGTA AAGCAGTCTG GCCTCGGCCG CGAAGGCTCC AAATATGGTA TCGAGGAATA TCTCGAAACC AAATATATCT GTAGCGCCTA TAAGCGGTGA AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA