BrabA.00019.a
Aldo/keto reductase
| CENTER ID: | BrabA.00019.a |
| ORGANISM: | Brucella melitensis biovar Abortus 2308 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.00019.a.A1.GE25369 | Full length( BrabA.00019.a ) | 1 | 294 | ||
| BrabA.00019.a.A2.GE28005 | Created from Decode construct for feature BrabA.00019.a | 1 | 279 | ||
| BrabA.00019.a.A3.GE28006 | Created from Decode construct for feature BrabA.00019.a | 19 | 294 | ||
| BrabA.00019.a.A4.GE28007 | Created from Decode construct for feature BrabA.00019.a | 19 | 279 | ||
| BrabA.00019.a.A5.GE28008 | Created from Decode construct for feature BrabA.00019.a | 24 | 294 | ||
| BrabA.00019.a.A6.GE28009 | Created from Decode construct for feature BrabA.00019.a | 24 | 279 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.00019.a.A2.PW27898 | Created from Decode construct for feature BrabA.00019.a | 1 | 279 | ||
| BrabA.00019.a.A3.PW27899 | Created from Decode construct for feature BrabA.00019.a | 19 | 294 | ||
| BrabA.00019.a.A4.PW27900 | Created from Decode construct for feature BrabA.00019.a | 19 | 279 | ||
| BrabA.00019.a.A5.PW27901 | Created from Decode construct for feature BrabA.00019.a | 24 | 294 | ||
| BrabA.00019.a.A6.PW27902 | Created from Decode construct for feature BrabA.00019.a | 24 | 279 |
Structures
3O0K
DEPOSITED: 7/19/2010
DETERMINATION: XRay
CLONE: BrabA.00019.a.A4.GE28007
PROTEIN: BrabA.00019.a.A4.PW27900
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126583 |
| PATRIC ID: | fig|359391.4.peg.2461 |
| RefSeq: | YP_418407 |
| UniProt: | Q2YII2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MWWPAPRKTA QKISGKGYIM TVPTVKLNDG NHIPQLGYGV WQISNDEAVS AVSEALKAGY RHIDTATIYG NEEGVGKAIN GSGIARADIF LTTKLWNSDQ GYESTLKAFD TSLKKLGTDY VDLYLIHWPM PSKDLFMETW RAFIKLKEEG RVKSIGVSNF RTADLERLIK ESGVTPVLNQ IELHPQFQQD ELRLFHGKHD IATEAWSPLG QGKLLEDPTL KSIAEKHAKS VAQIILRWHI ETGNIVIPKS ITPARIKENF DIFDFTLNGT DHDAITKLDM ADGRIGPNPS VFSA
NT Sequence
ATGTGGTGGC CAGCGCCCCG GAAAACGGCG CAAAAGATAA GTGGGAAAGG ATATATCATG ACCGTACCGA CGGTTAAACT GAACGACGGC AATCATATTC CGCAGCTCGG TTACGGTGTC TGGCAGATTA GCAATGATGA AGCGGTGAGC GCAGTCAGCG AGGCGCTGAA AGCCGGTTAT CGACACATCG ACACCGCCAC CATTTACGGC AATGAAGAAG GCGTCGGCAA GGCGATCAAC GGTTCGGGCA TCGCGCGCGC AGACATCTTC CTGACCACCA AGCTCTGGAA CAGCGATCAG GGCTATGAAT CCACGCTCAA GGCTTTCGAC ACCAGCCTGA AGAAGCTGGG CACCGACTAT GTGGATCTCT ATCTGATCCA CTGGCCCATG CCATCGAAAG ACCTGTTCAT GGAAACATGG CGCGCCTTCA TCAAACTGAA GGAAGAAGGC CGCGTCAAAT CCATCGGTGT ATCGAATTTC CGCACTGCCG ATCTTGAGCG CCTCATCAAG GAAAGCGGCG TTACGCCGGT CCTGAACCAG ATCGAGTTGC ACCCCCAGTT CCAGCAGGAT GAACTGCGCC TGTTCCATGG CAAGCACGAC ATCGCAACGG AAGCCTGGAG CCCGCTCGGC CAGGGCAAAC TTCTGGAAGA CCCGACGCTG AAATCGATTG CCGAAAAGCA CGCAAAGTCG GTGGCACAGA TCATTTTGCG CTGGCACATC GAGACCGGGA ATATCGTGAT CCCGAAATCG ATCACACCTG CCCGCATCAA GGAAAATTTC GATATTTTCG ACTTCACGCT GAACGGCACC GACCACGATG CCATCACCAA GCTTGACATG GCGGATGGCC GCATCGGGCC GAACCCGAGT GTTTTTTCAG CA
Details for BrabA.00019.a.A1.GE25369
| HARVESTED ON: | 6/30/2008 |
| SEQUENCED ON: | 6/16/2009 |
| EXPECTED MW: | 35kDa |
| OBSERVED MW: | 35kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 89 |
| PERCENT COVERAGE: | 94 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMWWPAPRKT AQKISGKGYI MTVPTVKLND GNHIPQLGYG VWQISNDEAV SAVSEALKAG YRHIDTATIY GNEEGVGKAI NGSGIARADI FLTTKLWNSD QGYESTLKAF DTSLKKLGTD YVDLYLIHWP MPSKDLFMET WRAFIKLKEE GRVKSIGVSN FRTADLERLI KESGVTPVLN QIELHPQFQQ DELRLFHGKH DIATEAWSPL GQGKLLEXPT LKSIAEKHAK SVAQIILRWH IETGXYRDPE IDHTCPHQGK FRYFRLHAER HRPRCXHQA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtggt ggccagcgcc ccggaaaacg gcgcaaaaga taagtgggaa aggatatatc atgaccgtac cgacggttaa actgaacgac ggcaatcata ttccgcagct cggttacggt gtctggcaga ttagcaatga tgaagcggtg agcgcagtca gcgaggcgct gaaagccggt tatcgacaca tcgacaccgc caccatttac ggcaatgaag aaggcgtcgg caaggcgatc aacggttcgg gcatcgcgcg cgcagacatc ttcctgacca ccaagctctg gaacagcgat cagggctatg aatccacgct caaggctttc gacaccagcc tgaagaagct gggcaccgac tatgtggatc tctatctgat ccactggccc atgccatcga aagacctgtt catggaaaca tggcgcgcct tcatcaaact gaaggaagaa ggccgcgtca aatccatcgg tgtatcgaat ttccgcactg ccgatcttga gcgcctcatc aaggaaagcg gcgttacgcc ggtcctgaac cagatcgagt tgcaccccca gttccagcag gatgaactgc gcctgttcca tggcaagcac gacatcgcaa cggaagcctg gagcccgctc ggccagggca aacttctgga aganccgacg ctgaaatcga ttgccgaaaa gcacgcaaag tcngtggcac agatcatttt gcgctggcac atcgagaccg ggnaatatcg tgatcccgaa atcgatcaca cctgcccgca tcaaggaaaa tttcgatatt ttcgacttca cgctgaacgg caccgaccac gatgccntca ccaagcttga c
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMWWPAPRKT AQKISGKGYI MTVPTVKLND GNHIPQLGYG VWQISNDEAV SAVSEALKAG YRHIDTATIY GNEEGVGKAI NGSGIARADI FLTTKLWNSD QGYESTLKAF DTSLKKLGTD YVDLYLIHWP MPSKDLFMET WRAFIKLKEE GRVKSIGVSN FRTADLERLI KESGVTPVLN QIELHPQFQQ DELRLFHGKH DIATEAWSPL GQGKLLEDPT LKSIAEKHAK SVAQIILRWH IETGNIVIPK SITPARIKEN FDIFDFTLNG TDHDAITKLD MADGRIGPNP SVFSA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtggtggcca gcgccccgga aaacggcgca aaagataagt gggaaaggat atatcatgac cgtaccgacg gttaaactga acgacggcaa tcatattccg cagctcggtt acggtgtctg gcagattagc aatgatgaag cggtgagcgc agtcagcgag gcgctgaaag ccggttatcg acacatcgac accgccacca tttacggcaa tgaagaaggc gtcggcaagg cgatcaacgg ttcgggcatc gcgcgcgcag acatcttcct gaccaccaag ctctggaaca gcgatcaggg ctatgaatcc acgctcaagg ctttcgacac cagcctgaag aagctgggca ccgactatgt ggatctctat ctgatccact ggcccatgcc atcgaaagac ctgttcatgg aaacatggcg cgccttcatc aaactgaagg aagaaggccg cgtcaaatcc atcggtgtat cgaatttccg cactgccgat cttgagcgcc tcatcaagga aagcggcgtt acgccggtcc tgaaccagat cgagttgcac ccccagttcc agcaggatga actgcgcctg ttccatggca agcacgacat cgcaacggaa gcctggagcc cgctcggcca gggcaaactt ctggaagacc cgacgctgaa atcgattgcc gaaaagcacg caaagtcggt ggcacagatc attttgcgct ggcacatcga gaccgggaat atcgtgatcc cgaaatcgat cacacctgcc cgcatcaagg aaaatttcga tattttcgac ttcacgctga acggcaccga ccacgatgcc atcaccaagc ttgacatggc ggatggccgc atcgggccga acccgagtgt tttttcagca taaaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for BrabA.00019.a.A2.GE28005
| HARVESTED ON: | 12/7/2009 |
| SEQUENCED ON: | 12/23/2009 |
| EXPECTED MW: | 33kDa |
| OBSERVED MW: | 34kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMWWPAPRKT AQKISGKGYI MTVPTVKLND GNHIPQLGYG VWQISNDEAV SAVSEALKAG YRHIDTATIY GNEEGVGKAI NGSGIARADI FLTTKLWNSD QGYESTLKAF DTSLKKLGTD YVDLYLIHWP MPSKDLFMET WRAFIKLKEE GRVKSIGVSN FRTADLERLI KESGVTPVLN QIELHPQFQQ DELRLFHGKH DIATEAWSPL GQGKLLEDPT LKSIAEKHAK SVAQIILRWH IETGNIVIPK SITPARIKEN FDIFDFTLNG TDHDAITKLD
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtggt ggccagcgcc ccggaaaacg gcgcaaaaga taagtgggaa aggatatatc atgaccgtac cgacggttaa actgaacgac ggcaatcata ttccgcagct cggttacggt gtctggcaga ttagcaatga tgaagcggtg agcgcagtca gcgaggcgct gaaagccggt tatcgacaca tcgacaccgc caccatttac ggcaatgaag aaggcgtcgg caaggcgatc aacggttcgg gcatcgcgcg cgcagacatc ttcctgacca ccaagctctg gaacagcgat cagggctatg aatccacgct caaggctttc gacaccagcc tgaagaagct gggcaccgac tatgtggatc tctatctgat ccactggccc atgccatcga aagacctgtt catggaaaca tggcgcgcct tcatcaaact gaaggaagaa ggccgcgtca aatccatcgg tgtatcgaat ttccgcactg ccgatcttga gcgcctcatc aaggaaagcg gcgttacgcc ggtcctgaac cagatcgagt tgcaccccca gttccagcag gatgaactgc gcctgttcca tggcaagcac gacatcgcaa cggaagcctg gagcccgctc ggccagggca aacttctgga agacccgacg ctgaaatcga ttgccgaaaa gcacgcaaag tcggtggcac agatcatttt gcgctggcac atcgagaccg ggaatatcgt gatcccgaaa tcgatcacac ctgcccgcat caaggaaaat ttcgatattt tcgacttcac gctgaacggc accgaccacg atgccatcac caagcttgac taataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMWWPAPRKT AQKISGKGYI MTVPTVKLND GNHIPQLGYG VWQISNDEAV SAVSEALKAG YRHIDTATIY GNEEGVGKAI NGSGIARADI FLTTKLWNSD QGYESTLKAF DTSLKKLGTD YVDLYLIHWP MPSKDLFMET WRAFIKLKEE GRVKSIGVSN FRTADLERLI KESGVTPVLN QIELHPQFQQ DELRLFHGKH DIATEAWSPL GQGKLLEDPT LKSIAEKHAK SVAQIILRWH IETGNIVIPK SITPARIKEN FDIFDFTLNG TDHDAITKLD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtggtggcca gcgccccgga aaacggcgca aaagataagt gggaaaggat atatcatgac cgtaccgacg gttaaactga acgacggcaa tcatattccg cagctcggtt acggtgtctg gcagattagc aatgatgaag cggtgagcgc agtcagcgag gcgctgaaag ccggttatcg acacatcgac accgccacca tttacggcaa tgaagaaggc gtcggcaagg cgatcaacgg ttcgggcatc gcgcgcgcag acatcttcct gaccaccaag ctctggaaca gcgatcaggg ctatgaatcc acgctcaagg ctttcgacac cagcctgaag aagctgggca ccgactatgt ggatctctat ctgatccact ggcccatgcc atcgaaagac ctgttcatgg aaacatggcg cgccttcatc aaactgaagg aagaaggccg cgtcaaatcc atcggtgtat cgaatttccg cactgccgat cttgagcgcc tcatcaagga aagcggcgtt acgccggtcc tgaaccagat cgagttgcac ccccagttcc agcaggatga actgcgcctg ttccatggca agcacgacat cgcaacggaa gcctggagcc cgctcggcca gggcaaactt ctggaagacc cgacgctgaa atcgattgcc gaaaagcacg caaagtcggt ggcacagatc attttgcgct ggcacatcga gaccgggaat atcgtgatcc cgaaatcgat cacacctgcc cgcatcaagg aaaatttcga tattttcgac ttcacgctga acggcaccga ccacgatgcc atcaccaagc ttgacaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BrabA.00019.a.A3.GE28006
| HARVESTED ON: | 12/7/2009 |
| SEQUENCED ON: | 12/23/2009 |
| EXPECTED MW: | 33kDa |
| OBSERVED MW: | 33kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMIMTVPTVK LNDGNHIPQL GYGVWQISND EAVSAVSEAL KAGYRHIDTA TIYGNEEGVG KAINGSGIAR ADIFLTTKLW NSDQGYESTL KAFDTSLKKL GTDYVDLYLI HWPMPSKDLF METWRAFIKL KEEGRVKSIG VSNFRTADLE RLIKESGVTP VLNQIELHPQ FQQDELRLFH GKHDIATEAW SPLGQGKLLE DPTLKSIAEK HAKSVAQIIL RWHIETGNIV IPKSITPARI KENFDIFDFT LNGTDHDAIT KLDMADGRIG PNPSVFSA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgatca tgaccgtacc gacggttaaa ctgaacgacg gcaatcatat tccgcagctc ggttacggtg tctggcagat tagcaatgat gaagcggtga gcgcagtcag cgaggcgctg aaagccggtt atcgacacat cgacaccgcc accatttacg gcaatgaaga aggcgtcggc aaggcgatca acggttcggg catcgcgcgc gcagacatct tcctgaccac caagctctgg aacagcgatc agggctatga atccacgctc aaggctttcg acaccagcct gaagaagctg ggcaccgact atgtggatct ctatctgatc cactggccca tgccatcgaa agacctgttc atggaaacat ggcgcgcctt catcaaactg aaggaagaag gccgcgtcaa atccatcggt gtatcgaatt tccgcactgc cgatcttgag cgcctcatca aggaaagcgg cgttacgccg gtcctgaacc agatcgagtt gcacccccag ttccagcagg atgaactgcg cctgttccat ggcaagcacg acatcgcaac ggaagcctgg agcccgctcg gccagggcaa acttctggaa gacccgacgc tgaaatcgat tgccgaaaag cacgcaaagt cggtggcaca gatcattttg cgctggcaca tcgagaccgg gaatatcgtg atcccgaaat cgatcacacc tgcccgcatc aaggaaaatt tcgatatttt cgacttcacg ctgaacggca ccgaccacga tgccatcacc aagcttgaca tggcggatgg ccgcatcggg ccgaacccga gtgttttttc agcataataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIMTVPTVK LNDGNHIPQL GYGVWQISND EAVSAVSEAL KAGYRHIDTA TIYGNEEGVG KAINGSGIAR ADIFLTTKLW NSDQGYESTL KAFDTSLKKL GTDYVDLYLI HWPMPSKDLF METWRAFIKL KEEGRVKSIG VSNFRTADLE RLIKESGVTP VLNQIELHPQ FQQDELRLFH GKHDIATEAW SPLGQGKLLE DPTLKSIAEK HAKSVAQIIL RWHIETGNIV IPKSITPARI KENFDIFDFT LNGTDHDAIT KLDMADGRIG PNPSVFSA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatcatgacc gtaccgacgg ttaaactgaa cgacggcaat catattccgc agctcggtta cggtgtctgg cagattagca atgatgaagc ggtgagcgca gtcagcgagg cgctgaaagc cggttatcga cacatcgaca ccgccaccat ttacggcaat gaagaaggcg tcggcaaggc gatcaacggt tcgggcatcg cgcgcgcaga catcttcctg accaccaagc tctggaacag cgatcagggc tatgaatcca cgctcaaggc tttcgacacc agcctgaaga agctgggcac cgactatgtg gatctctatc tgatccactg gcccatgcca tcgaaagacc tgttcatgga aacatggcgc gccttcatca aactgaagga agaaggccgc gtcaaatcca tcggtgtatc gaatttccgc actgccgatc ttgagcgcct catcaaggaa agcggcgtta cgccggtcct gaaccagatc gagttgcacc cccagttcca gcaggatgaa ctgcgcctgt tccatggcaa gcacgacatc gcaacggaag cctggagccc gctcggccag ggcaaacttc tggaagaccc gacgctgaaa tcgattgccg aaaagcacgc aaagtcggtg gcacagatca ttttgcgctg gcacatcgag accgggaata tcgtgatccc gaaatcgatc acacctgccc gcatcaagga aaatttcgat attttcgact tcacgctgaa cggcaccgac cacgatgcca tcaccaagct tgacatggcg gatggccgca tcgggccgaa cccgagtgtt ttttcagcaa aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for BrabA.00019.a.A4.GE28007
| HARVESTED ON: | 12/7/2009 |
| SEQUENCED ON: | 12/23/2009 |
| EXPECTED MW: | 31kDa |
| OBSERVED MW: | 32kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMIMTVPTVK LNDGNHIPQL GYGVWQISND EAVSAVSEAL KAGYRHIDTA TIYGNEEGVG KAINGSGIAR ADIFLTTKLW NSDQGYESTL KAFDTSLKKL GTDYVDLYLI HWPMPSKDLF METWRAFIKL KEEGRVKSIG VSNFRTADLE RLIKESGVTP VLNQIELHPQ FQQDELRLFH GKHDIATEAW SPLGQGKLLE DPTLKSIAEK HAKSVAQIIL RWHIETGNIV IPKSITPARI KENFDIFDFT LNGTDHDAIT KLD
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgatca tgaccgtacc gacggttaaa ctgaacgacg gcaatcatat tccgcagctc ggttacggtg tctggcagat tagcaatgat gaagcggtga gcgcagtcag cgaggcgctg aaagccggtt atcgacacat cgacaccgcc accatttacg gcaatgaaga aggcgtcggc aaggcgatca acggttcggg catcgcgcgc gcagacatct tcctgaccac caagctctgg aacagcgatc agggctatga atccacgctc aaggctttcg acaccagcct gaagaagctg ggcaccgact atgtggatct ctatctgatc cactggccca tgccatcgaa agacctgttc atggaaacat ggcgcgcctt catcaaactg aaggaagaag gccgcgtcaa atccatcggt gtatcgaatt tccgcactgc cgatcttgag cgcctcatca aggaaagcgg cgttacgccg gtcctgaacc agatcgagtt gcacccccag ttccagcagg atgaactgcg cctgttccat ggcaagcacg acatcgcaac ggaagcctgg agcccgctcg gccagggcaa acttctggaa gacccgacgc tgaaatcgat tgccgaaaag cacgcaaagt cggtggcaca gatcattttg cgctggcaca tcgagaccgg gaatatcgtg atcccgaaat cgatcacacc tgcccgcatc aaggaaaatt tcgatatttt cgacttcacg ctgaacggca ccgaccacga tgccatcacc aagcttgact aataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIMTVPTVK LNDGNHIPQL GYGVWQISND EAVSAVSEAL KAGYRHIDTA TIYGNEEGVG KAINGSGIAR ADIFLTTKLW NSDQGYESTL KAFDTSLKKL GTDYVDLYLI HWPMPSKDLF METWRAFIKL KEEGRVKSIG VSNFRTADLE RLIKESGVTP VLNQIELHPQ FQQDELRLFH GKHDIATEAW SPLGQGKLLE DPTLKSIAEK HAKSVAQIIL RWHIETGNIV IPKSITPARI KENFDIFDFT LNGTDHDAIT KLD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatcatgacc gtaccgacgg ttaaactgaa cgacggcaat catattccgc agctcggtta cggtgtctgg cagattagca atgatgaagc ggtgagcgca gtcagcgagg cgctgaaagc cggttatcga cacatcgaca ccgccaccat ttacggcaat gaagaaggcg tcggcaaggc gatcaacggt tcgggcatcg cgcgcgcaga catcttcctg accaccaagc tctggaacag cgatcagggc tatgaatcca cgctcaaggc tttcgacacc agcctgaaga agctgggcac cgactatgtg gatctctatc tgatccactg gcccatgcca tcgaaagacc tgttcatgga aacatggcgc gccttcatca aactgaagga agaaggccgc gtcaaatcca tcggtgtatc gaatttccgc actgccgatc ttgagcgcct catcaaggaa agcggcgtta cgccggtcct gaaccagatc gagttgcacc cccagttcca gcaggatgaa ctgcgcctgt tccatggcaa gcacgacatc gcaacggaag cctggagccc gctcggccag ggcaaacttc tggaagaccc gacgctgaaa tcgattgccg aaaagcacgc aaagtcggtg gcacagatca ttttgcgctg gcacatcgag accgggaata tcgtgatccc gaaatcgatc acacctgccc gcatcaagga aaatttcgat attttcgact tcacgctgaa cggcaccgac cacgatgcca tcaccaagct tgacaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for BrabA.00019.a.A5.GE28008
| HARVESTED ON: | 12/7/2009 |
| SEQUENCED ON: | 12/23/2009 |
| EXPECTED MW: | 32kDa |
| OBSERVED MW: | 33kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTVKLNDGN HIPQLGYGVW QISNDEAVSA VSEALKAGYR HIDTATIYGN EEGVGKAING SGIARADIFL TTKLWNSDQG YESTLKAFDT SLKKLGTDYV DLYLIHWPMP SKDLFMETWR AFIKLKEEGR VKSIGVSNFR TADLERLIKE SGVTPVLNQI ELHPQFQQDE LRLFHGKHDI ATEAWSPLGQ GKLLEDPTLK SIAEKHAKSV AQIILRWHIE TGNIVIPKSI TPARIKENFD IFDFTLNGTD HDAITKLDMA DGRIGPNPSV FSA
Validated NT Sequence
taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg acggttaaac tgaacgacgg caatcatatt ccgcagctcg gttacggtgt ctggcagatt agcaatgatg aagcggtgag cgcagtcagc gaggcgctga aagccggtta tcgacacatc gacaccgcca ccatttacgg caatgaagaa ggcgtcggca aggcgatcaa cggttcgggc atcgcgcgcg cagacatctt cctgaccacc aagctctgga acagcgatca gggctatgaa tccacgctca aggctttcga caccagcctg aagaagctgg gcaccgacta tgtggatctc tatctgatcc actggcccat gccatcgaaa gacctgttca tggaaacatg gcgcgccttc atcaaactga aggaagaagg ccgcgtcaaa tccatcggtg tatcgaattt ccgcactgcc gatcttgagc gcctcatcaa ggaaagcggc gttacgccgg tcctgaacca gatcgagttg cacccccagt tccagcagga tgaactgcgc ctgttccatg gcaagcacga catcgcaacg gaagcctgga gcccgctcgg ccagggcaaa cttctggaag acccgacgct gaaatcgatt gccgaaaagc acgcaaagtc ggtggcacag atcattttgc gctggcacat cgagaccggg aatatcgtga tcccgaaatc gatcacacct gcccgcatca aggaaaattt cgatattttc gacttcacgc tgaacggcac cgaccacgat gccatcacca agcttgacat ggcggatggc cgcatcgggc cgaacccgag tgttttttca gcataataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTVKLNDGN HIPQLGYGVW QISNDEAVSA VSEALKAGYR HIDTATIYGN EEGVGKAING SGIARADIFL TTKLWNSDQG YESTLKAFDT SLKKLGTDYV DLYLIHWPMP SKDLFMETWR AFIKLKEEGR VKSIGVSNFR TADLERLIKE SGVTPVLNQI ELHPQFQQDE LRLFHGKHDI ATEAWSPLGQ GKLLEDPTLK SIAEKHAKSV AQIILRWHIE TGNIVIPKSI TPARIKENFD IFDFTLNGTD HDAITKLDMA DGRIGPNPSV FSA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacggttaaa ctgaacgacg gcaatcatat tccgcagctc ggttacggtg tctggcagat tagcaatgat gaagcggtga gcgcagtcag cgaggcgctg aaagccggtt atcgacacat cgacaccgcc accatttacg gcaatgaaga aggcgtcggc aaggcgatca acggttcggg catcgcgcgc gcagacatct tcctgaccac caagctctgg aacagcgatc agggctatga atccacgctc aaggctttcg acaccagcct gaagaagctg ggcaccgact atgtggatct ctatctgatc cactggccca tgccatcgaa agacctgttc atggaaacat ggcgcgcctt catcaaactg aaggaagaag gccgcgtcaa atccatcggt gtatcgaatt tccgcactgc cgatcttgag cgcctcatca aggaaagcgg cgttacgccg gtcctgaacc agatcgagtt gcacccccag ttccagcagg atgaactgcg cctgttccat ggcaagcacg acatcgcaac ggaagcctgg agcccgctcg gccagggcaa acttctggaa gacccgacgc tgaaatcgat tgccgaaaag cacgcaaagt cggtggcaca gatcattttg cgctggcaca tcgagaccgg gaatatcgtg atcccgaaat cgatcacacc tgcccgcatc aaggaaaatt tcgatatttt cgacttcacg ctgaacggca ccgaccacga tgccatcacc aagcttgaca tggcggatgg ccgcatcggg ccgaacccga gtgttttttc agcaaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for BrabA.00019.a.A6.GE28009
| HARVESTED ON: | 12/7/2009 |
| SEQUENCED ON: | 12/23/2009 |
| EXPECTED MW: | 31kDa |
| OBSERVED MW: | 31kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTVKLNDGN HIPQLGYGVW QISNDEAVSA VSEALKAGYR HIDTATIYGN EEGVGKAING SGIARADIFL TTKLWNSDQG YESTLKAFDT SLKKLGTDYV DLYLIHWPMP SKDLFMETWR AFIKLKEEGR VKSIGVSNFR TADLERLIKE SGVTPVLNQI ELHPQFQQDE LRLFHGKHDI ATEAWSPLGQ GKLLEDPTLK SIAEKHAKSV AQIILRWHIE TGNIVIPKSI TPARIKENFD IFDFTLNGTD HDAITKLD
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacgg ttaaactgaa cgacggcaat catattccgc agctcggtta cggtgtctgg cagattagca atgatgaagc ggtgagcgca gtcagcgagg cgctgaaagc cggttatcga cacatcgaca ccgccaccat ttacggcaat gaagaaggcg tcggcaaggc gatcaacggt tcgggcatcg cgcgcgcaga catcttcctg accaccaagc tctggaacag cgatcagggc tatgaatcca cgctcaaggc tttcgacacc agcctgaaga agctgggcac cgactatgtg gatctctatc tgatccactg gcccatgcca tcgaaagacc tgttcatgga aacatggcgc gccttcatca aactgaagga agaaggccgc gtcaaatcca tcggtgtatc gaatttccgc actgccgatc ttgagcgcct catcaaggaa agcggcgtta cgccggtcct gaaccagatc gagttgcacc cccagttcca gcaggatgaa ctgcgcctgt tccatggcaa gcacgacatc gcaacggaag cctggagccc gctcggccag ggcaaacttc tggaagaccc gacgctgaaa tcgattgccg aaaagcacgc aaagtcggtg gcacagatca ttttgcgctg gcacatcgag accgggaata tcgtgatccc gaaatcgatc acacctgccc gcatcaagga aaatttcgat attttcgact tcacgctgaa cggcaccgac cacgatgcca tcaccaagct tgactaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTVKLNDGN HIPQLGYGVW QISNDEAVSA VSEALKAGYR HIDTATIYGN EEGVGKAING SGIARADIFL TTKLWNSDQG YESTLKAFDT SLKKLGTDYV DLYLIHWPMP SKDLFMETWR AFIKLKEEGR VKSIGVSNFR TADLERLIKE SGVTPVLNQI ELHPQFQQDE LRLFHGKHDI ATEAWSPLGQ GKLLEDPTLK SIAEKHAKSV AQIILRWHIE TGNIVIPKSI TPARIKENFD IFDFTLNGTD HDAITKLD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacggttaaa ctgaacgacg gcaatcatat tccgcagctc ggttacggtg tctggcagat tagcaatgat gaagcggtga gcgcagtcag cgaggcgctg aaagccggtt atcgacacat cgacaccgcc accatttacg gcaatgaaga aggcgtcggc aaggcgatca acggttcggg catcgcgcgc gcagacatct tcctgaccac caagctctgg aacagcgatc agggctatga atccacgctc aaggctttcg acaccagcct gaagaagctg ggcaccgact atgtggatct ctatctgatc cactggccca tgccatcgaa agacctgttc atggaaacat ggcgcgcctt catcaaactg aaggaagaag gccgcgtcaa atccatcggt gtatcgaatt tccgcactgc cgatcttgag cgcctcatca aggaaagcgg cgttacgccg gtcctgaacc agatcgagtt gcacccccag ttccagcagg atgaactgcg cctgttccat ggcaagcacg acatcgcaac ggaagcctgg agcccgctcg gccagggcaa acttctggaa gacccgacgc tgaaatcgat tgccgaaaag cacgcaaagt cggtggcaca gatcattttg cgctggcaca tcgagaccgg gaatatcgtg atcccgaaat cgatcacacc tgcccgcatc aaggaaaatt tcgatatttt cgacttcacg ctgaacggca ccgaccacga tgccatcacc aagcttgaca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for BrabA.00019.a.A2.PW27898
| PURIFICATION DATe: | 2/1/2010 |
| CONCENTRATION: | 26mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 8 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMWWPAPRKT AQKISGKGYI MTVPTVKLND GNHIPQLGYG VWQISNDEAV SAVSEALKAG YRHIDTATIY GNEEGVGKAI NGSGIARADI FLTTKLWNSD QGYESTLKAF DTSLKKLGTD YVDLYLIHWP MPSKDLFMET WRAFIKLKEE GRVKSIGVSN FRTADLERLI KESGVTPVLN QIELHPQFQQ DELRLFHGKH DIATEAWSPL GQGKLLEDPT LKSIAEKHAK SVAQIILRWH IETGNIVIPK SITPARIKEN FDIFDFTLNG TDHDAITKLD
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtggt ggccagcgcc ccggaaaacg gcgcaaaaga taagtgggaa aggatatatc atgaccgtac cgacggttaa actgaacgac ggcaatcata ttccgcagct cggttacggt gtctggcaga ttagcaatga tgaagcggtg agcgcagtca gcgaggcgct gaaagccggt tatcgacaca tcgacaccgc caccatttac ggcaatgaag aaggcgtcgg caaggcgatc aacggttcgg gcatcgcgcg cgcagacatc ttcctgacca ccaagctctg gaacagcgat cagggctatg aatccacgct caaggctttc gacaccagcc tgaagaagct gggcaccgac tatgtggatc tctatctgat ccactggccc atgccatcga aagacctgtt catggaaaca tggcgcgcct tcatcaaact gaaggaagaa ggccgcgtca aatccatcgg tgtatcgaat ttccgcactg ccgatcttga gcgcctcatc aaggaaagcg gcgttacgcc ggtcctgaac cagatcgagt tgcaccccca gttccagcag gatgaactgc gcctgttcca tggcaagcac gacatcgcaa cggaagcctg gagcccgctc ggccagggca aacttctgga agacccgacg ctgaaatcga ttgccgaaaa gcacgcaaag tcggtggcac agatcatttt gcgctggcac atcgagaccg ggaatatcgt gatcccgaaa tcgatcacac ctgcccgcat caaggaaaat ttcgatattt tcgacttcac gctgaacggc accgaccacg atgccatcac caagcttgac taataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMWWPAPRKT AQKISGKGYI MTVPTVKLND GNHIPQLGYG VWQISNDEAV SAVSEALKAG YRHIDTATIY GNEEGVGKAI NGSGIARADI FLTTKLWNSD QGYESTLKAF DTSLKKLGTD YVDLYLIHWP MPSKDLFMET WRAFIKLKEE GRVKSIGVSN FRTADLERLI KESGVTPVLN QIELHPQFQQ DELRLFHGKH DIATEAWSPL GQGKLLEDPT LKSIAEKHAK SVAQIILRWH IETGNIVIPK SITPARIKEN FDIFDFTLNG TDHDAITKLD
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTGGTGGCCA GCGCCCCGGA AAACGGCGCA AAAGATAAGT GGGAAAGGAT ATATCATGAC CGTACCGACG GTTAAACTGA ACGACGGCAA TCATATTCCG CAGCTCGGTT ACGGTGTCTG GCAGATTAGC AATGATGAAG CGGTGAGCGC AGTCAGCGAG GCGCTGAAAG CCGGTTATCG ACACATCGAC ACCGCCACCA TTTACGGCAA TGAAGAAGGC GTCGGCAAGG CGATCAACGG TTCGGGCATC GCGCGCGCAG ACATCTTCCT GACCACCAAG CTCTGGAACA GCGATCAGGG CTATGAATCC ACGCTCAAGG CTTTCGACAC CAGCCTGAAG AAGCTGGGCA CCGACTATGT GGATCTCTAT CTGATCCACT GGCCCATGCC ATCGAAAGAC CTGTTCATGG AAACATGGCG CGCCTTCATC AAACTGAAGG AAGAAGGCCG CGTCAAATCC ATCGGTGTAT CGAATTTCCG CACTGCCGAT CTTGAGCGCC TCATCAAGGA AAGCGGCGTT ACGCCGGTCC TGAACCAGAT CGAGTTGCAC CCCCAGTTCC AGCAGGATGA ACTGCGCCTG TTCCATGGCA AGCACGACAT CGCAACGGAA GCCTGGAGCC CGCTCGGCCA GGGCAAACTT CTGGAAGACC CGACGCTGAA ATCGATTGCC GAAAAGCACG CAAAGTCGGT GGCACAGATC ATTTTGCGCT GGCACATCGA GACCGGGAAT ATCGTGATCC CGAAATCGAT CACACCTGCC CGCATCAAGG AAAATTTCGA TATTTTCGAC TTCACGCTGA ACGGCACCGA CCACGATGCC ATCACCAAGC TTGACAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA
Details for BrabA.00019.a.A3.PW27899
| PURIFICATION DATe: | 2/1/2010 |
| CONCENTRATION: | 28.94mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 12 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMIMTVPTVK LNDGNHIPQL GYGVWQISND EAVSAVSEAL KAGYRHIDTA TIYGNEEGVG KAINGSGIAR ADIFLTTKLW NSDQGYESTL KAFDTSLKKL GTDYVDLYLI HWPMPSKDLF METWRAFIKL KEEGRVKSIG VSNFRTADLE RLIKESGVTP VLNQIELHPQ FQQDELRLFH GKHDIATEAW SPLGQGKLLE DPTLKSIAEK HAKSVAQIIL RWHIETGNIV IPKSITPARI KENFDIFDFT LNGTDHDAIT KLDMADGRIG PNPSVFSA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgatca tgaccgtacc gacggttaaa ctgaacgacg gcaatcatat tccgcagctc ggttacggtg tctggcagat tagcaatgat gaagcggtga gcgcagtcag cgaggcgctg aaagccggtt atcgacacat cgacaccgcc accatttacg gcaatgaaga aggcgtcggc aaggcgatca acggttcggg catcgcgcgc gcagacatct tcctgaccac caagctctgg aacagcgatc agggctatga atccacgctc aaggctttcg acaccagcct gaagaagctg ggcaccgact atgtggatct ctatctgatc cactggccca tgccatcgaa agacctgttc atggaaacat ggcgcgcctt catcaaactg aaggaagaag gccgcgtcaa atccatcggt gtatcgaatt tccgcactgc cgatcttgag cgcctcatca aggaaagcgg cgttacgccg gtcctgaacc agatcgagtt gcacccccag ttccagcagg atgaactgcg cctgttccat ggcaagcacg acatcgcaac ggaagcctgg agcccgctcg gccagggcaa acttctggaa gacccgacgc tgaaatcgat tgccgaaaag cacgcaaagt cggtggcaca gatcattttg cgctggcaca tcgagaccgg gaatatcgtg atcccgaaat cgatcacacc tgcccgcatc aaggaaaatt tcgatatttt cgacttcacg ctgaacggca ccgaccacga tgccatcacc aagcttgaca tggcggatgg ccgcatcggg ccgaacccga gtgttttttc agcataataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIMTVPTVK LNDGNHIPQL GYGVWQISND EAVSAVSEAL KAGYRHIDTA TIYGNEEGVG KAINGSGIAR ADIFLTTKLW NSDQGYESTL KAFDTSLKKL GTDYVDLYLI HWPMPSKDLF METWRAFIKL KEEGRVKSIG VSNFRTADLE RLIKESGVTP VLNQIELHPQ FQQDELRLFH GKHDIATEAW SPLGQGKLLE DPTLKSIAEK HAKSVAQIIL RWHIETGNIV IPKSITPARI KENFDIFDFT LNGTDHDAIT KLDMADGRIG PNPSVFSA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GATCATGACC GTACCGACGG TTAAACTGAA CGACGGCAAT CATATTCCGC AGCTCGGTTA CGGTGTCTGG CAGATTAGCA ATGATGAAGC GGTGAGCGCA GTCAGCGAGG CGCTGAAAGC CGGTTATCGA CACATCGACA CCGCCACCAT TTACGGCAAT GAAGAAGGCG TCGGCAAGGC GATCAACGGT TCGGGCATCG CGCGCGCAGA CATCTTCCTG ACCACCAAGC TCTGGAACAG CGATCAGGGC TATGAATCCA CGCTCAAGGC TTTCGACACC AGCCTGAAGA AGCTGGGCAC CGACTATGTG GATCTCTATC TGATCCACTG GCCCATGCCA TCGAAAGACC TGTTCATGGA AACATGGCGC GCCTTCATCA AACTGAAGGA AGAAGGCCGC GTCAAATCCA TCGGTGTATC GAATTTCCGC ACTGCCGATC TTGAGCGCCT CATCAAGGAA AGCGGCGTTA CGCCGGTCCT GAACCAGATC GAGTTGCACC CCCAGTTCCA GCAGGATGAA CTGCGCCTGT TCCATGGCAA GCACGACATC GCAACGGAAG CCTGGAGCCC GCTCGGCCAG GGCAAACTTC TGGAAGACCC GACGCTGAAA TCGATTGCCG AAAAGCACGC AAAGTCGGTG GCACAGATCA TTTTGCGCTG GCACATCGAG ACCGGGAATA TCGTGATCCC GAAATCGATC ACACCTGCCC GCATCAAGGA AAATTTCGAT ATTTTCGACT TCACGCTGAA CGGCACCGAC CACGATGCCA TCACCAAGCT TGACATGGCG GATGGCCGCA TCGGGCCGAA CCCGAGTGTT TTTTCAGCAA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A
Details for BrabA.00019.a.A4.PW27900
| PURIFICATION DATe: | 2/1/2010 |
| CONCENTRATION: | 25.56mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 16 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMIMTVPTVK LNDGNHIPQL GYGVWQISND EAVSAVSEAL KAGYRHIDTA TIYGNEEGVG KAINGSGIAR ADIFLTTKLW NSDQGYESTL KAFDTSLKKL GTDYVDLYLI HWPMPSKDLF METWRAFIKL KEEGRVKSIG VSNFRTADLE RLIKESGVTP VLNQIELHPQ FQQDELRLFH GKHDIATEAW SPLGQGKLLE DPTLKSIAEK HAKSVAQIIL RWHIETGNIV IPKSITPARI KENFDIFDFT LNGTDHDAIT KLD
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgatca tgaccgtacc gacggttaaa ctgaacgacg gcaatcatat tccgcagctc ggttacggtg tctggcagat tagcaatgat gaagcggtga gcgcagtcag cgaggcgctg aaagccggtt atcgacacat cgacaccgcc accatttacg gcaatgaaga aggcgtcggc aaggcgatca acggttcggg catcgcgcgc gcagacatct tcctgaccac caagctctgg aacagcgatc agggctatga atccacgctc aaggctttcg acaccagcct gaagaagctg ggcaccgact atgtggatct ctatctgatc cactggccca tgccatcgaa agacctgttc atggaaacat ggcgcgcctt catcaaactg aaggaagaag gccgcgtcaa atccatcggt gtatcgaatt tccgcactgc cgatcttgag cgcctcatca aggaaagcgg cgttacgccg gtcctgaacc agatcgagtt gcacccccag ttccagcagg atgaactgcg cctgttccat ggcaagcacg acatcgcaac ggaagcctgg agcccgctcg gccagggcaa acttctggaa gacccgacgc tgaaatcgat tgccgaaaag cacgcaaagt cggtggcaca gatcattttg cgctggcaca tcgagaccgg gaatatcgtg atcccgaaat cgatcacacc tgcccgcatc aaggaaaatt tcgatatttt cgacttcacg ctgaacggca ccgaccacga tgccatcacc aagcttgact aataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIMTVPTVK LNDGNHIPQL GYGVWQISND EAVSAVSEAL KAGYRHIDTA TIYGNEEGVG KAINGSGIAR ADIFLTTKLW NSDQGYESTL KAFDTSLKKL GTDYVDLYLI HWPMPSKDLF METWRAFIKL KEEGRVKSIG VSNFRTADLE RLIKESGVTP VLNQIELHPQ FQQDELRLFH GKHDIATEAW SPLGQGKLLE DPTLKSIAEK HAKSVAQIIL RWHIETGNIV IPKSITPARI KENFDIFDFT LNGTDHDAIT KLD
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GATCATGACC GTACCGACGG TTAAACTGAA CGACGGCAAT CATATTCCGC AGCTCGGTTA CGGTGTCTGG CAGATTAGCA ATGATGAAGC GGTGAGCGCA GTCAGCGAGG CGCTGAAAGC CGGTTATCGA CACATCGACA CCGCCACCAT TTACGGCAAT GAAGAAGGCG TCGGCAAGGC GATCAACGGT TCGGGCATCG CGCGCGCAGA CATCTTCCTG ACCACCAAGC TCTGGAACAG CGATCAGGGC TATGAATCCA CGCTCAAGGC TTTCGACACC AGCCTGAAGA AGCTGGGCAC CGACTATGTG GATCTCTATC TGATCCACTG GCCCATGCCA TCGAAAGACC TGTTCATGGA AACATGGCGC GCCTTCATCA AACTGAAGGA AGAAGGCCGC GTCAAATCCA TCGGTGTATC GAATTTCCGC ACTGCCGATC TTGAGCGCCT CATCAAGGAA AGCGGCGTTA CGCCGGTCCT GAACCAGATC GAGTTGCACC CCCAGTTCCA GCAGGATGAA CTGCGCCTGT TCCATGGCAA GCACGACATC GCAACGGAAG CCTGGAGCCC GCTCGGCCAG GGCAAACTTC TGGAAGACCC GACGCTGAAA TCGATTGCCG AAAAGCACGC AAAGTCGGTG GCACAGATCA TTTTGCGCTG GCACATCGAG ACCGGGAATA TCGTGATCCC GAAATCGATC ACACCTGCCC GCATCAAGGA AAATTTCGAT ATTTTCGACT TCACGCTGAA CGGCACCGAC CACGATGCCA TCACCAAGCT TGACAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA
Details for BrabA.00019.a.A5.PW27901
| PURIFICATION DATe: | 2/1/2010 |
| CONCENTRATION: | 27.07mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 17 |
| VIAL VOLUME: | data unavailable |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTVKLNDGN HIPQLGYGVW QISNDEAVSA VSEALKAGYR HIDTATIYGN EEGVGKAING SGIARADIFL TTKLWNSDQG YESTLKAFDT SLKKLGTDYV DLYLIHWPMP SKDLFMETWR AFIKLKEEGR VKSIGVSNFR TADLERLIKE SGVTPVLNQI ELHPQFQQDE LRLFHGKHDI ATEAWSPLGQ GKLLEDPTLK SIAEKHAKSV AQIILRWHIE TGNIVIPKSI TPARIKENFD IFDFTLNGTD HDAITKLDMA DGRIGPNPSV FSA
Validated NT Sequence
taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg acggttaaac tgaacgacgg caatcatatt ccgcagctcg gttacggtgt ctggcagatt agcaatgatg aagcggtgag cgcagtcagc gaggcgctga aagccggtta tcgacacatc gacaccgcca ccatttacgg caatgaagaa ggcgtcggca aggcgatcaa cggttcgggc atcgcgcgcg cagacatctt cctgaccacc aagctctgga acagcgatca gggctatgaa tccacgctca aggctttcga caccagcctg aagaagctgg gcaccgacta tgtggatctc tatctgatcc actggcccat gccatcgaaa gacctgttca tggaaacatg gcgcgccttc atcaaactga aggaagaagg ccgcgtcaaa tccatcggtg tatcgaattt ccgcactgcc gatcttgagc gcctcatcaa ggaaagcggc gttacgccgg tcctgaacca gatcgagttg cacccccagt tccagcagga tgaactgcgc ctgttccatg gcaagcacga catcgcaacg gaagcctgga gcccgctcgg ccagggcaaa cttctggaag acccgacgct gaaatcgatt gccgaaaagc acgcaaagtc ggtggcacag atcattttgc gctggcacat cgagaccggg aatatcgtga tcccgaaatc gatcacacct gcccgcatca aggaaaattt cgatattttc gacttcacgc tgaacggcac cgaccacgat gccatcacca agcttgacat ggcggatggc cgcatcgggc cgaacccgag tgttttttca gcataataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTVKLNDGN HIPQLGYGVW QISNDEAVSA VSEALKAGYR HIDTATIYGN EEGVGKAING SGIARADIFL TTKLWNSDQG YESTLKAFDT SLKKLGTDYV DLYLIHWPMP SKDLFMETWR AFIKLKEEGR VKSIGVSNFR TADLERLIKE SGVTPVLNQI ELHPQFQQDE LRLFHGKHDI ATEAWSPLGQ GKLLEDPTLK SIAEKHAKSV AQIILRWHIE TGNIVIPKSI TPARIKENFD IFDFTLNGTD HDAITKLDMA DGRIGPNPSV FSA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACGGTTAAA CTGAACGACG GCAATCATAT TCCGCAGCTC GGTTACGGTG TCTGGCAGAT TAGCAATGAT GAAGCGGTGA GCGCAGTCAG CGAGGCGCTG AAAGCCGGTT ATCGACACAT CGACACCGCC ACCATTTACG GCAATGAAGA AGGCGTCGGC AAGGCGATCA ACGGTTCGGG CATCGCGCGC GCAGACATCT TCCTGACCAC CAAGCTCTGG AACAGCGATC AGGGCTATGA ATCCACGCTC AAGGCTTTCG ACACCAGCCT GAAGAAGCTG GGCACCGACT ATGTGGATCT CTATCTGATC CACTGGCCCA TGCCATCGAA AGACCTGTTC ATGGAAACAT GGCGCGCCTT CATCAAACTG AAGGAAGAAG GCCGCGTCAA ATCCATCGGT GTATCGAATT TCCGCACTGC CGATCTTGAG CGCCTCATCA AGGAAAGCGG CGTTACGCCG GTCCTGAACC AGATCGAGTT GCACCCCCAG TTCCAGCAGG ATGAACTGCG CCTGTTCCAT GGCAAGCACG ACATCGCAAC GGAAGCCTGG AGCCCGCTCG GCCAGGGCAA ACTTCTGGAA GACCCGACGC TGAAATCGAT TGCCGAAAAG CACGCAAAGT CGGTGGCACA GATCATTTTG CGCTGGCACA TCGAGACCGG GAATATCGTG ATCCCGAAAT CGATCACACC TGCCCGCATC AAGGAAAATT TCGATATTTT CGACTTCACG CTGAACGGCA CCGACCACGA TGCCATCACC AAGCTTGACA TGGCGGATGG CCGCATCGGG CCGAACCCGA GTGTTTTTTC AGCAAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA
Details for BrabA.00019.a.A6.PW27902
| PURIFICATION DATe: | 2/1/2010 |
| CONCENTRATION: | 26.14mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 11 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTVKLNDGN HIPQLGYGVW QISNDEAVSA VSEALKAGYR HIDTATIYGN EEGVGKAING SGIARADIFL TTKLWNSDQG YESTLKAFDT SLKKLGTDYV DLYLIHWPMP SKDLFMETWR AFIKLKEEGR VKSIGVSNFR TADLERLIKE SGVTPVLNQI ELHPQFQQDE LRLFHGKHDI ATEAWSPLGQ GKLLEDPTLK SIAEKHAKSV AQIILRWHIE TGNIVIPKSI TPARIKENFD IFDFTLNGTD HDAITKLD
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacgg ttaaactgaa cgacggcaat catattccgc agctcggtta cggtgtctgg cagattagca atgatgaagc ggtgagcgca gtcagcgagg cgctgaaagc cggttatcga cacatcgaca ccgccaccat ttacggcaat gaagaaggcg tcggcaaggc gatcaacggt tcgggcatcg cgcgcgcaga catcttcctg accaccaagc tctggaacag cgatcagggc tatgaatcca cgctcaaggc tttcgacacc agcctgaaga agctgggcac cgactatgtg gatctctatc tgatccactg gcccatgcca tcgaaagacc tgttcatgga aacatggcgc gccttcatca aactgaagga agaaggccgc gtcaaatcca tcggtgtatc gaatttccgc actgccgatc ttgagcgcct catcaaggaa agcggcgtta cgccggtcct gaaccagatc gagttgcacc cccagttcca gcaggatgaa ctgcgcctgt tccatggcaa gcacgacatc gcaacggaag cctggagccc gctcggccag ggcaaacttc tggaagaccc gacgctgaaa tcgattgccg aaaagcacgc aaagtcggtg gcacagatca ttttgcgctg gcacatcgag accgggaata tcgtgatccc gaaatcgatc acacctgccc gcatcaagga aaatttcgat attttcgact tcacgctgaa cggcaccgac cacgatgcca tcaccaagct tgactaataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTVKLNDGN HIPQLGYGVW QISNDEAVSA VSEALKAGYR HIDTATIYGN EEGVGKAING SGIARADIFL TTKLWNSDQG YESTLKAFDT SLKKLGTDYV DLYLIHWPMP SKDLFMETWR AFIKLKEEGR VKSIGVSNFR TADLERLIKE SGVTPVLNQI ELHPQFQQDE LRLFHGKHDI ATEAWSPLGQ GKLLEDPTLK SIAEKHAKSV AQIILRWHIE TGNIVIPKSI TPARIKENFD IFDFTLNGTD HDAITKLD
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACGGTTAAA CTGAACGACG GCAATCATAT TCCGCAGCTC GGTTACGGTG TCTGGCAGAT TAGCAATGAT GAAGCGGTGA GCGCAGTCAG CGAGGCGCTG AAAGCCGGTT ATCGACACAT CGACACCGCC ACCATTTACG GCAATGAAGA AGGCGTCGGC AAGGCGATCA ACGGTTCGGG CATCGCGCGC GCAGACATCT TCCTGACCAC CAAGCTCTGG AACAGCGATC AGGGCTATGA ATCCACGCTC AAGGCTTTCG ACACCAGCCT GAAGAAGCTG GGCACCGACT ATGTGGATCT CTATCTGATC CACTGGCCCA TGCCATCGAA AGACCTGTTC ATGGAAACAT GGCGCGCCTT CATCAAACTG AAGGAAGAAG GCCGCGTCAA ATCCATCGGT GTATCGAATT TCCGCACTGC CGATCTTGAG CGCCTCATCA AGGAAAGCGG CGTTACGCCG GTCCTGAACC AGATCGAGTT GCACCCCCAG TTCCAGCAGG ATGAACTGCG CCTGTTCCAT GGCAAGCACG ACATCGCAAC GGAAGCCTGG AGCCCGCTCG GCCAGGGCAA ACTTCTGGAA GACCCGACGC TGAAATCGAT TGCCGAAAAG CACGCAAAGT CGGTGGCACA GATCATTTTG CGCTGGCACA TCGAGACCGG GAATATCGTG ATCCCGAAAT CGATCACACC TGCCCGCATC AAGGAAAATT TCGATATTTT CGACTTCACG CTGAACGGCA CCGACCACGA TGCCATCACC AAGCTTGACA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A