BrabA.00014.a
Isocitrate lyase and phosphorylmutase:Isocitrate lyase
| CENTER ID: | BrabA.00014.a |
| ORGANISM: | Brucella melitensis biovar Abortus 2308 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.00014.a.A1.GE25213 | Full length( BrabA.00014.a ) | 1 | 429 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.00014.a.A1.PW38950 | Full length( BrabA.00014.a ) | 1 | 429 |
Structures
3E5B
DEPOSITED: 8/13/2008
DETERMINATION: XRay
CLONE: BrabA.00014.a.A1.GE25213
PROTEIN: BrabA.00014.a.A1.PW25251
3EOL
DEPOSITED: 9/28/2008
DETERMINATION: XRay
CLONE: BrabA.00014.a.A1.GE25213
PROTEIN: BrabA.00014.a.A1.PW25251
3OQ8
DEPOSITED: 9/2/2010
DETERMINATION: XRay
CLONE: BrabA.00014.a.A1.GE25213
PROTEIN: BrabA.00014.a.A1.PW25251
3P0X
DEPOSITED: 9/29/2010
DETERMINATION: XRay
CLONE: BrabA.00014.a.A1.GE25213
PROTEIN: BrabA.00014.a.A1.PW25251
7RBX
DEPOSITED: 7/6/2021
DETERMINATION: XRay
CLONE: BrabA.00014.a.A1.GE25213
PROTEIN: BrabA.00014.a.A1.PW38950
Publications by SSGCID
Aconitate decarboxylase 1 participates in the control of pulmonary Brucella infection in mice
Abendroth J, Edwards T, Demars A, Vitali A, Comein A, Carlier E, Azouz A, Goriely S, Smout J, Flamand V, Van Gysel M, Wouters J, Machelart A, Hoffmann E, Brodin P, De Bolle X, Muraille E
PLoS Pathog. - 2021
volume 17, issue 9,
PMID: 34525130; PMCID: PMC8443048
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_129572 |
| PATRIC ID: | fig|359391.4.peg.1690 |
| RefSeq: | YP_414989 |
| UniProt: | Q2YQA0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDFYSLIPS APKGRFDGIE RAHTAEDVKR LRGSVEIKYS LAEMGANRLW KLIHEEDFVN ALGALSGNQA MQMVRAGLKA IYLSGWQVAA DANTASAMYP DQSLYPANAG PELAKRINRT LQRADQIETA EGKGLSVDTW FAPIVADAEA GFGGPLDAFE IMKAYIEAGA AGVHFEDQLA SEKKCGHLGG KVLIPTAAHI RNLNAARLAA DVMGTPTLIV ARTDAEAAKL LTSDIDERDQ PFVDYEAGRT AEGFYQVKNG IEPCIARAIA YAPYCDLIWM ETSKPDLAQA RRFAEAVHKA HPGKLLAYNC SPSFNWKKNL DDATIAKFQR ELGAMGYKFQ FITLAGFHQL NYGMFELARG YKDRQMAAYS ELQQAEFAAE ADGYTATKHQ REVGTGYFDA VSLAITGGQS STTAMKESTE TAQFKPAAE
NT Sequence
ATGACAGATT TTTACAGCCT CATCCCTTCG GCACCCAAGG GCCGCTTCGA CGGCATTGAA CGTGCGCATA CGGCCGAAGA CGTGAAAAGG CTGCGCGGTT CGGTGGAGAT AAAGTATTCG CTGGCCGAAA TGGGTGCAAA CCGCCTCTGG AAGCTGATCC ACGAGGAAGA TTTCGTCAAT GCGCTGGGCG CGCTTTCCGG CAACCAGGCC ATGCAGATGG TTCGCGCCGG GTTGAAGGCG ATCTACCTTT CCGGCTGGCA GGTTGCAGCA GACGCCAACA CGGCATCAGC CATGTATCCC GACCAGTCGC TCTATCCGGC CAATGCCGGG CCAGAGCTTG CAAAGCGCAT CAACCGCACG CTGCAACGCG CCGACCAGAT CGAAACCGCC GAAGGCAAGG GGCTTTCGGT TGACACATGG TTCGCGCCCA TCGTGGCGGA TGCCGAGGCA GGCTTTGGCG GCCCGCTGGA TGCCTTCGAG ATCATGAAAG CCTATATCGA GGCCGGTGCT GCGGGCGTTC ACTTTGAAGA CCAGCTTGCT TCGGAAAAGA AGTGCGGCCA TCTGGGCGGC AAGGTTCTGA TCCCGACCGC AGCGCACATC CGCAACCTCA ACGCGGCACG CCTTGCAGCC GACGTCATGG GCACGCCGAC CCTCATCGTG GCGCGCACGG ATGCGGAAGC AGCCAAGCTT CTGACGTCGG ATATCGACGA GCGCGACCAG CCTTTCGTCG ATTACGAAGC AGGCCGCACG GCGGAAGGCT TCTATCAGGT GAAGAATGGC ATCGAGCCAT GCATCGCCCG TGCGATTGCC TATGCGCCTT ATTGCGATCT GATCTGGATG GAAACGTCCA AGCCTGATCT CGCACAGGCC CGTCGCTTCG CGGAAGCCGT GCACAAGGCG CATCCGGGCA AGCTGCTCGC CTATAACTGC TCGCCGTCGT TCAACTGGAA AAAGAACCTC GACGACGCGA CGATTGCCAA GTTCCAGCGC GAGCTGGGCG CGATGGGCTA CAAGTTCCAG TTCATCACGC TGGCTGGCTT CCACCAGCTC AACTACGGCA TGTTCGAGCT GGCACGCGGC TACAAGGATC GTCAGATGGC GGCTTATTCC GAGCTGCAAC AGGCGGAATT TGCAGCCGAA GCCGATGGCT ACACGGCAAC CAAGCACCAG CGCGAAGTCG GCACCGGCTA TTTCGATGCC GTGTCGCTCG CAATCACCGG CGGCCAGTCT TCGACCACCG CCATGAAGGA ATCGACCGAA ACCGCACAGT TCAAGCCGGC TGCCGAG
Details for BrabA.00014.a.A1.GE25213
| HARVESTED ON: | 5/6/2008 |
| SEQUENCED ON: | 3/16/2012 |
| EXPECTED MW: | 49kDa |
| OBSERVED MW: | 50kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 68 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTDFYSLIP SAPKGRFDGI ERAHTAEDVK RLRGSVEIKY SLAEMGANRL WKLIHEEDFV NALGALSGNQ AMQMVRAGLK AIYLSGWQVA ADANTASAMY PDQSLYPANA GPELAKRINR TLQRADQIET AEGKGLSVDT WFAPIVADAE AGFGGPLDAF EIMKAYIEAG AAGVHFEDQL ASEKKCGHLG GKVLIPTAAH IRNLNAARLA ADVMGTPTLI VARTDAEAAK LLTSDIDERD QPFVDYEAXR TAEGFYXXKN GIEPCIARAI AYAPYCDLIW METSKPDLA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacag atttttacag cctcatccct tcggcaccca agggccgctt cgacggcatt gaacgtgcgc atacggccga agacgtgaaa aggctgcgcg gttcggtgga gataaagtat tcgctggccg aaatgggtgc aaaccgcctc tggaagctga tccacgagga agatttcgtc aatgcgctgg gcgcgctttc cggcaaccag gccatgcaga tggttcgcgc cgggttgaag gcgatctacc tttccggctg gcaggttgca gcagacgcca acacggcatc agccatgtat cccgaccagt cgctctatcc ggccaatgcc gggccagagc ttgcaaagcg catcaaccgc acgctgcaac gcgccgacca gatcgaaacc gccgaaggca aggggctttc ggttgacaca tggttcgcgc ccatcgtggc ggatgccgag gcaggctttg gcggcccgct ggatgccttc gagatcatga aagcctatat cgaggccggc gccgcgggcg ttcactttga agaccagctt gcttcggaaa agaagtgcgg ccatctgggc ggcaaggttc tgatcccgac cgcagcgcac atccgcaacc tcaacgcggc acgccttgca gccgacgtca tgggcacgcc gaccctcatc gtggcgcgca cggatgcgga agcagccaag ctgctgacgt cggacatcga cgagcgcgac cagcctttcg tcgattacga agcannncgc acggcggaag gnttctatca ngngaagaat ggcatcgagc catgcatcgc ccgtgcgatt gcctatgcgc cttattgcga tctgatctgg atggaaacgt ccaagcctga tctcgcac
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDFYSLIP SAPKGRFDGI ERAHTAEDVK RLRGSVEIKY SLAEMGANRL WKLIHEEDFV NALGALSGNQ AMQMVRAGLK AIYLSGWQVA ADANTASAMY PDQSLYPANA GPELAKRINR TLQRADQIET AEGKGLSVDT WFAPIVADAE AGFGGPLDAF EIMKAYIEAG AAGVHFEDQL ASEKKCGHLG GKVLIPTAAH IRNLNAARLA ADVMGTPTLI VARTDAEAAK LLTSDIDERD QPFVDYEAGR TAEGFYQVKN GIEPCIARAI AYAPYCDLIW METSKPDLAQ ARRFAEAVHK AHPGKLLAYN CSPSFNWKKN LDDATIAKFQ RELGAMGYKF QFITLAGFHQ LNYGMFELAR GYKDRQMAAY SELQQAEFAA EADGYTATKH QREVGTGYFD AVSLAITGGQ SSTTAMKEST ETAQFKPAAE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacagatttt tacagcctca tcccttcggc acccaagggc cgcttcgacg gcattgaacg tgcgcatacg gccgaagacg tgaaaaggct gcgcggttcg gtggagataa agtattcgct ggccgaaatg ggtgcaaacc gcctctggaa gctgatccac gaggaagatt tcgtcaatgc gctgggcgcg ctttccggca accaggccat gcagatggtt cgcgccgggt tgaaggcgat ctacctttcc ggctggcagg ttgcagcaga cgccaacacg gcatcagcca tgtatcccga ccagtcgctc tatccggcca atgccgggcc agagcttgca aagcgcatca accgcacgct gcaacgcgcc gaccagatcg aaaccgccga aggcaagggg ctttcggttg acacatggtt cgcgcccatc gtggcggatg ccgaggcagg ctttggcggc ccgctggatg ccttcgagat catgaaagcc tatatcgagg ccggcgccgc gggcgttcac tttgaagacc agcttgcttc ggaaaagaag tgcggccatc tgggcggcaa ggttctgatc ccgaccgcag cgcacatccg caacctcaac gcggcacgcc ttgcagccga cgtcatgggc acgccgaccc tcatcgtggc gcgcacggat gcggaagcag ccaagctgct gacgtcggac atcgacgagc gcgaccagcc tttcgtcgat tacgaagcag gccgcacggc ggaaggcttc tatcaggtga agaatggcat cgagccatgc atcgcccgtg cgattgccta tgcgccttat tgcgatctga tctggatgga aacgtccaag cctgatctcg cacaggcccg tcgcttcgcg gaagccgtgc acaaggcgca tccgggcaag ctgctcgcct ataactgctc gccgtcgttc aactggaaaa agaacctcga cgacgcgacg attgccaagt tccagcgcga gctgggcgcg atgggctaca agttccagtt catcacgctg gctggcttcc accagctcaa ctacggcatg ttcgagctgg cacgcggcta caaggatcgt cagatggcgg cttattccga gctgcaacag gcggaatttg cagccgaagc cgatggctac acggcaacca agcaccagcg cgaagtcggc accggctatt tcgatgccgt gtcgctcgca atcaccggcg gccagtcttc gaccaccgcc atgaaggaat cgaccgaaac cgcacagttc aagccggctg ccgagtagaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for BrabA.00014.a.A1.PW38950
| PURIFICATION DATe: | 3/2/2021 |
| CONCENTRATION: | 102mg/ml |
| OBSERVED MW: | 49kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 8 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 68 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTDFYSLIP SAPKGRFDGI ERAHTAEDVK RLRGSVEIKY SLAEMGANRL WKLIHEEDFV NALGALSGNQ AMQMVRAGLK AIYLSGWQVA ADANTASAMY PDQSLYPANA GPELAKRINR TLQRADQIET AEGKGLSVDT WFAPIVADAE AGFGGPLDAF EIMKAYIEAG AAGVHFEDQL ASEKKCGHLG GKVLIPTAAH IRNLNAARLA ADVMGTPTLI VARTDAEAAK LLTSDIDERD QPFVDYEAXR TAEGFYXXKN GIEPCIARAI AYAPYCDLIW METSKPDLA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacag atttttacag cctcatccct tcggcaccca agggccgctt cgacggcatt gaacgtgcgc atacggccga agacgtgaaa aggctgcgcg gttcggtgga gataaagtat tcgctggccg aaatgggtgc aaaccgcctc tggaagctga tccacgagga agatttcgtc aatgcgctgg gcgcgctttc cggcaaccag gccatgcaga tggttcgcgc cgggttgaag gcgatctacc tttccggctg gcaggttgca gcagacgcca acacggcatc agccatgtat cccgaccagt cgctctatcc ggccaatgcc gggccagagc ttgcaaagcg catcaaccgc acgctgcaac gcgccgacca gatcgaaacc gccgaaggca aggggctttc ggttgacaca tggttcgcgc ccatcgtggc ggatgccgag gcaggctttg gcggcccgct ggatgccttc gagatcatga aagcctatat cgaggccggc gccgcgggcg ttcactttga agaccagctt gcttcggaaa agaagtgcgg ccatctgggc ggcaaggttc tgatcccgac cgcagcgcac atccgcaacc tcaacgcggc acgccttgca gccgacgtca tgggcacgcc gaccctcatc gtggcgcgca cggatgcgga agcagccaag ctgctgacgt cggacatcga cgagcgcgac cagcctttcg tcgattacga agcannncgc acggcggaag gnttctatca ngngaagaat ggcatcgagc catgcatcgc ccgtgcgatt gcctatgcgc cttattgcga tctgatctgg atggaaacgt ccaagcctga tctcgcac
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDFYSLIP SAPKGRFDGI ERAHTAEDVK RLRGSVEIKY SLAEMGANRL WKLIHEEDFV NALGALSGNQ AMQMVRAGLK AIYLSGWQVA ADANTASAMY PDQSLYPANA GPELAKRINR TLQRADQIET AEGKGLSVDT WFAPIVADAE AGFGGPLDAF EIMKAYIEAG AAGVHFEDQL ASEKKCGHLG GKVLIPTAAH IRNLNAARLA ADVMGTPTLI VARTDAEAAK LLTSDIDERD QPFVDYEAGR TAEGFYQVKN GIEPCIARAI AYAPYCDLIW METSKPDLAQ ARRFAEAVHK AHPGKLLAYN CSPSFNWKKN LDDATIAKFQ RELGAMGYKF QFITLAGFHQ LNYGMFELAR GYKDRQMAAY SELQQAEFAA EADGYTATKH QREVGTGYFD AVSLAITGGQ SSTTAMKEST ETAQFKPAAE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACAGATTTT TACAGCCTCA TCCCTTCGGC ACCCAAGGGC CGCTTCGACG GCATTGAACG TGCGCATACG GCCGAAGACG TGAAAAGGCT GCGCGGTTCG GTGGAGATAA AGTATTCGCT GGCCGAAATG GGTGCAAACC GCCTCTGGAA GCTGATCCAC GAGGAAGATT TCGTCAATGC GCTGGGCGCG CTTTCCGGCA ACCAGGCCAT GCAGATGGTT CGCGCCGGGT TGAAGGCGAT CTACCTTTCC GGCTGGCAGG TTGCAGCAGA CGCCAACACG GCATCAGCCA TGTATCCCGA CCAGTCGCTC TATCCGGCCA ATGCCGGGCC AGAGCTTGCA AAGCGCATCA ACCGCACGCT GCAACGCGCC GACCAGATCG AAACCGCCGA AGGCAAGGGG CTTTCGGTTG ACACATGGTT CGCGCCCATC GTGGCGGATG CCGAGGCAGG CTTTGGCGGC CCGCTGGATG CCTTCGAGAT CATGAAAGCC TATATCGAGG CCGGCGCCGC GGGCGTTCAC TTTGAAGACC AGCTTGCTTC GGAAAAGAAG TGCGGCCATC TGGGCGGCAA GGTTCTGATC CCGACCGCAG CGCACATCCG CAACCTCAAC GCGGCACGCC TTGCAGCCGA CGTCATGGGC ACGCCGACCC TCATCGTGGC GCGCACGGAT GCGGAAGCAG CCAAGCTGCT GACGTCGGAC ATCGACGAGC GCGACCAGCC TTTCGTCGAT TACGAAGCAG GCCGCACGGC GGAAGGCTTC TATCAGGTGA AGAATGGCAT CGAGCCATGC ATCGCCCGTG CGATTGCCTA TGCGCCTTAT TGCGATCTGA TCTGGATGGA AACGTCCAAG CCTGATCTCG CACAGGCCCG TCGCTTCGCG GAAGCCGTGC ACAAGGCGCA TCCGGGCAAG CTGCTCGCCT ATAACTGCTC GCCGTCGTTC AACTGGAAAA AGAACCTCGA CGACGCGACG ATTGCCAAGT TCCAGCGCGA GCTGGGCGCG ATGGGCTACA AGTTCCAGTT CATCACGCTG GCTGGCTTCC ACCAGCTCAA CTACGGCATG TTCGAGCTGG CACGCGGCTA CAAGGATCGT CAGATGGCGG CTTATTCCGA GCTGCAACAG GCGGAATTTG CAGCCGAAGC CGATGGCTAC ACGGCAACCA AGCACCAGCG CGAAGTCGGC ACCGGCTATT TCGATGCCGT GTCGCTCGCA ATCACCGGCG GCCAGTCTTC GACCACCGCC ATGAAGGAAT CGACCGAAAC CGCACAGTTC AAGCCGGCTG CCGAGTAGAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA