SSGCID: Target Page

BrabA.00010.k

3-hydroxyacyl-CoA dehydrogenase

CENTER ID:	BrabA.00010.k
ORGANISM:	Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrabA.00010.k.B1.GE37692	Full length( BrabA.00010.k )		1	209

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruMel86222_3084

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MALGASARFQ RTDVASDTDG KAAIAAAIEA FSRIDVLVNC AGVAPGEKVL GREGAHKLET FTRTISINLI GTFNMLRLAA EAMAKNEPGQ GGERGVIINT ASVAAFDGQI GQAAYSASKG GVAAMTLPVA RELARHGIRV MTIAPGIFKT PMMAGMPQEV QDALGASVPF PPRLGEPAEY AALVRHIVEN QMLNGEVIRL DGALRMAAK

NT Sequence

TTGGCGCTTG GCGCTTCGGC CAGATTCCAG CGCACAGACG TCGCAAGCGA TACCGACGGC AAGGCTGCAA TTGCAGCAGC GATTGAAGCT TTTAGCCGCA TCGACGTACT GGTCAATTGC GCCGGCGTCG CGCCGGGCGA AAAGGTGCTT GGCCGCGAAG GCGCACACAA GCTTGAAACC TTCACCCGCA CCATTTCGAT CAACCTGATC GGCACCTTCA ACATGCTGCG CCTTGCAGCC GAAGCCATGG CGAAGAACGA ACCCGGCCAG GGCGGCGAGC GCGGTGTGAT CATCAACACG GCTTCCGTTG CCGCTTTTGA CGGCCAGATC GGCCAGGCTG CCTATTCGGC CTCCAAGGGT GGCGTTGCGG CCATGACGCT ACCCGTCGCG CGCGAACTTG CCCGCCACGG CATTCGTGTG ATGACCATTG CGCCCGGCAT TTTCAAGACC CCGATGATGG CAGGAATGCC GCAGGAAGTG CAGGACGCGC TGGGTGCTTC CGTGCCTTTC CCACCGCGTC TTGGCGAACC GGCGGAATAT GCCGCCCTCG TCCGCCATAT CGTGGAAAAC CAGATGTTGA ACGGCGAAGT GATCCGTCTC GACGGCGCAC TGCGTATGGC CGCCAAG

Details for BrabA.00010.k.B1.GE37692

HARVESTED ON:	2/5/2014
SEQUENCED ON:	2/6/2014
EXPECTED MW:	23kDa
OBSERVED MW:	23kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML ALGASARFQR TDVASDTDGK AAIAAAIEAF SRIDVLVNCA GVAPGEKVLG REGAHKLETF TRTISINLIG TFNMLRLAAE AMAKNEPGQG GERGVIINTA SVAAFDGQIG QAAYSASKGG VAAMTLPVAR ELARHGIRVM TIAPGIFKTP MMAGMPQEVQ DALGASVPFP PRLGEPAEYA ALVRHIVENQ MLNGEVIRLD GALRMAAK

Validated NT Sequence

ctctagatnn ttttgtttaa ctttaagaag gagatatacc atggctcacc accaccacca ccatatgttg gcgcttggcg cttcggccag attccagcgc acagacgtcg caagcgatac cgacggcaag gctgcaattg cagcagcgat tgaagctttt agccgcatcg acgtactggt caattgcgcc ggcgtcgcgc cgggcgaaaa ggtgcttggc cgcgaaggcg cacacaagct tgaaaccttc acccgcacca tttcgatcaa cctgatcggc accttcaaca tgctgcgcct tgcagccgaa gccatggcga agaacgaacc cggccagggc ggcgagcgcg gtgtgatcat caacacggct tccgttgccg cttttgacgg ccagatcggc caggctgcct attcggcctc caagggtggc gttgcggcca tgacgctacc cgtcgcgcgc gaacttgccc gccacggcat tcgtgtgatg accattgcgc ccggcatttt caagaccccg atgatggcag gaatgccgca ggaagtgcag gacgcgctgg gtgcttccgt gcctttccca ccgcgtcttg gcgaaccggc ggaatatgcc gccctcgtcc gccatatcgt ggaaaaccag atgttgaacg gcgaagtgat ccgtctcgac ggcgcactgc gtatggccgc caagtaagtg agtaagatag gatccggctg tannaccccc caaaaaggaa aaanncaagn nnnaannggc gccaccgagt ttggcccctg ccgctctaac ggtcctgggg gctgcccatc tannacaacg aacgcgcccc cccccgcgca ctgcggctag atgattccgc canattgctc gacncccccn tggttgggct tgcccctgca cgttgaccac atcacnnaac ttgcacgcca gctctagacc tggtcgcatc gctgantagg ctngccccgc cactccgcaa gcccttgntt cgccgtccgn ntnnnccccg gaaaanngaa aatacccccc acccnncaca ncctnccggn caaccaancc ncnaaantgc tttccacgtt ncnggcctag gaccacccgg taantgagta aataagganc gnnnnncccc

Expected Protein Sequence

MAHHHHHHMA LGASARFQRT DVASDTDGKA AIAAAIEAFS RIDVLVNCAG VAPGEKVLGR EGAHKLETFT RTISINLIGT FNMLRLAAEA MAKNEPGQGG ERGVIINTAS VAAFDGQIGQ AAYSASKGGV AAMTLPVARE LARHGIRVMT IAPGIFKTPM MAGMPQEVQD ALGASVPFPP RLGEPAEYAA LVRHIVENQM LNGEVIRLDG ALRMAAK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttggcgc ttggcgcttc ggccagattc cagcgcacag acgtcgcaag cgataccgac ggcaaggctg caattgcagc agcgattgaa gcttttagcc gcatcgacgt actggtcaat tgcgccggcg tcgcgccggg cgaaaaggtg cttggccgcg aaggcgcaca caagcttgaa accttcaccc gcaccatttc gatcaacctg atcggcacct tcaacatgct gcgccttgca gccgaagcca tggcgaagaa cgaacccggc cagggcggcg agcgcggtgt gatcatcaac acggcttccg ttgccgcttt tgacggccag atcggccagg ctgcctattc ggcctccaag ggtggcgttg cggccatgac gctacccgtc gcgcgcgaac ttgcccgcca cggcattcgt gtgatgacca ttgcgcccgg cattttcaag accccgatga tggcaggaat gccgcaggaa gtgcaggacg cgctgggtgc ttccgtgcct ttcccaccgc gtcttggcga accggcggaa tatgccgccc tcgtccgcca tatcgtggaa aaccagatgt tgaacggcga agtgatccgt ctcgacggcg cactgcgtat ggccgccaag tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat