BrabA.00010.h

FolM Alternative dihydrofolate reductase 1

CENTER ID: BrabA.00010.h
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.00010.h.B1.GE37690 Full length( BrabA.00010.h ) 1 259
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruMel86222_0749
BV-BRC: fig|359391.4.peg.749
RefSeq: WP_002963842.1
RefSeq: YP_414163.1
UniProt: Q2YN34

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLNDPEARMV ANCPVLVTGG ARRIGKAIVE DLARHGFPVA IHCNRSLDEG EAIVNRINDS GGNACVVQAD LEGDVRGLVK QASDRIGPIR LLVNNASLFQ EDKVGALDMA LWDRHFAVHL KTPVILAEDM PKALPEDQDG LVVNIIDQRV WKLNPQFFSY TLSKTALWNA TRTLAQALAP RIRVNAIAPG PTLPSERQRP EDFERQVSKL PLQRAPELPE FGRTVRYFWE NRSITGQMIA LDGGQHLAWE TPDIAGITE
NT Sequence
GTGTTGAATG ATCCCGAAGC CCGCATGGTT GCAAATTGCC CCGTCCTCGT GACAGGCGGC GCACGGCGTA TTGGGAAGGC GATCGTCGAA GACCTCGCCA GACATGGTTT TCCGGTTGCC ATTCATTGCA ATCGCTCGCT TGATGAAGGC GAGGCAATTG TAAATCGCAT CAACGACAGT GGCGGCAATG CCTGCGTGGT TCAAGCCGAC CTTGAAGGCG ATGTGCGTGG TTTGGTGAAA CAGGCCTCGG ACCGGATCGG CCCCATTCGC CTGCTCGTCA ACAATGCTTC CCTGTTTCAG GAGGATAAGG TCGGCGCCCT TGATATGGCC TTGTGGGACC GGCATTTCGC CGTTCACCTG AAAACGCCTG TGATTCTGGC AGAAGACATG CCCAAAGCCC TGCCGGAAGA TCAGGACGGG CTGGTGGTCA ATATCATCGA CCAGCGCGTG TGGAAGCTCA ATCCGCAGTT CTTTTCCTAT ACGCTGTCCA AAACGGCTTT GTGGAACGCG ACGCGCACGC TGGCGCAGGC TTTGGCGCCA CGCATCCGCG TCAACGCCAT CGCGCCCGGC CCCACCTTGC CCAGCGAGCG CCAGCGGCCG GAAGATTTCG AGCGGCAGGT TAGCAAACTT CCCCTCCAGC GCGCACCGGA ATTGCCGGAA TTCGGGCGTA CGGTTCGCTA TTTCTGGGAA AACCGTTCCA TTACCGGCCA GATGATTGCG CTCGACGGCG GGCAGCACCT GGCATGGGAA ACGCCCGACA TTGCAGGAAT TACCGAA
Details for BrabA.00010.h.B1.GE37690
HARVESTED ON: 2/5/2014
SEQUENCED ON: 2/6/2014
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 70
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMV LNDPEARMVA NCPVLVTGGA RRIGKAIVED LARHGFPVAI HCNRSLDEGE AIVNRINDSG GNACVVQADL EGDVRGLVKQ ASDRIGPIRL LVNNASLFQE DKVGALDMAL WDRHFAVHLK TPVILAEDMP KALPEDQDGL VVNIIDQRVW KLNPQFFSYT LSKRLXGTRR ARWRRXWXHA SASTXXAXXX FPASAXGXXF RXXGXXXPXX XXEXXXRXXV AIXGXRSXPX XXXXXGXXXX KXXFXXPXXX
Validated NT Sequence
cnnnnntctc tagatatttt gnttncttta agaaggagat ataccatggc tcaccaccac caccaccata tggtgttgaa tgatcccgaa gcccgcatgg ttgcaaattg ccccgtcctc gtgacaggcg gcgcacggcg tattgggaag gcgatcgtcg aagacctcgc cagacatggt tttccggttg ccattcattg caatcgctcg cttgatgaag gcgaggcaat tgtaaatcgc atcaacgaca gtggcggcaa tgcctgcgtg gttcaagccg accttgaagg cgatgtgcgt ggtttggtga aacaggcctc ggaccggatc ggccccattc gcctgctcgt caacaatgct tccctgtttc aggaggataa ggtcggcgcc cttgatatgg ccttgtggga ccggcatttc gccgttcacc tgaaaacgcc tgtgattctg gcagaagaca tgcccaaagc cctgccggaa gatcaggacg ggctggtggt caatatcatc gaccagcgcg tgtggaagct caatccgcag ttcttttcct atacgctgtc caaacggctt tntggaacgc gacgcgcacg ctggcgcagg ntttggngcc acgcatccgc gtcaacncnt cnngcccngn ccnncttccc agcgagcgcc agnggnngga nntttcgagn nnnnggtann nanttnccnn cnnnnnnnnc cgaantnnnn natcggnnnn nggtcgctat tnngggaanc cgttcnnnnc cgnnnannnt tgnnnnnngn ggnnnnncnn nnnnnaaanc cnnctttnnn nttcccnaan nnnnnaa
Expected Protein Sequence
MAHHHHHHML NDPEARMVAN CPVLVTGGAR RIGKAIVEDL ARHGFPVAIH CNRSLDEGEA IVNRINDSGG NACVVQADLE GDVRGLVKQA SDRIGPIRLL VNNASLFQED KVGALDMALW DRHFAVHLKT PVILAEDMPK ALPEDQDGLV VNIIDQRVWK LNPQFFSYTL SKTALWNATR TLAQALAPRI RVNAIAPGPT LPSERQRPED FERQVSKLPL QRAPELPEFG RTVRYFWENR SITGQMIALD GGQHLAWETP DIAGITE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgttga atgatcccga agcccgcatg gttgcaaatt gccccgtcct cgtgacaggc ggcgcacggc gtattgggaa ggcgatcgtc gaagacctcg ccagacatgg ttttccggtt gccattcatt gcaatcgctc gcttgatgaa ggcgaggcaa ttgtaaatcg catcaacgac agtggcggca atgcctgcgt ggttcaagcc gaccttgaag gcgatgtgcg tggtttggtg aaacaggcct cggaccggat cggccccatt cgcctgctcg tcaacaatgc ttccctgttt caggaggata aggtcggcgc ccttgatatg gccttgtggg accggcattt cgccgttcac ctgaaaacgc ctgtgattct ggcagaagac atgcccaaag ccctgccgga agatcaggac gggctggtgg tcaatatcat cgaccagcgc gtgtggaagc tcaatccgca gttcttttcc tatacgctgt ccaaaacggc tttgtggaac gcgacgcgca cgctggcgca ggctttggcg ccacgcatcc gcgtcaacgc catcgcgccc ggccccacct tgcccagcga gcgccagcgg ccggaagatt tcgagcggca ggttagcaaa cttcccctcc agcgcgcacc ggaattgccg gaattcgggc gtacggttcg ctatttctgg gaaaaccgtt ccattaccgg ccagatgatt gcgctcgacg gcgggcagca cctggcatgg gaaacgcccg acattgcagg aattaccgaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat