BrabA.00010.e
7-alpha-hydroxysteroid dehydrogenase
| CENTER ID: | BrabA.00010.e |
| ORGANISM: | Brucella melitensis biovar Abortus 2308 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.00010.e.A1.GE25556 | Full length( BrabA.00010.e ) | 1 | 255 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
3GAF
DEPOSITED: 2/17/2009
DETERMINATION: XRay
CLONE: BrabA.00010.e.A1.GE25556
PROTEIN: BrabA.00010.e.A1.PW25601
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_129079 |
| PATRIC ID: | fig|359391.4.peg.1693 |
| RefSeq: | YP_414992.1 |
| UniProt: | Q2YQ97 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSYESPFHLN DAVAIVTGAA AGIGRAIAGT FAKAGASVVV TDLKSEGAEA VAAAIRQAGG KAIGLECNVT DEQHREAVIK AALDQFGKIT VLVNNAGGGG PKPFDMPMSD FEWAFKLNLF SLFRLSQLAA PHMQKAGGGA ILNISSMAGE NTNVRMASYG SSKAAVNHLT RNIAFDVGPM GIRVNAIAPG AIKTDALATV LTPEIERAML KHTPLGRLGE AQDIANAALF LCSPAAAWIS GQVLTVSGGG VQELD
NT Sequence
ATGTCCTATG AGAGCCCGTT CCACCTGAAT GATGCTGTTG CAATCGTCAC CGGAGCGGCG GCAGGTATCG GACGGGCCAT CGCCGGAACC TTCGCCAAGG CTGGTGCTTC GGTTGTCGTA ACCGACCTTA AAAGCGAAGG CGCTGAAGCC GTTGCAGCCG CAATAAGGCA AGCTGGCGGC AAGGCCATCG GGCTTGAATG CAACGTAACC GACGAGCAGC ATCGCGAAGC CGTCATCAAA GCGGCCCTCG ATCAGTTCGG CAAAATCACT GTTCTGGTCA ACAATGCGGG TGGAGGCGGC CCCAAGCCCT TCGATATGCC GATGAGCGAC TTTGAATGGG CCTTCAAGCT CAATCTCTTT TCCCTGTTTC GCCTGTCGCA ATTGGCTGCC CCACACATGC AGAAGGCTGG CGGCGGCGCA ATTCTCAATA TTTCGTCCAT GGCCGGAGAA AATACCAATG TCCGGATGGC CTCCTATGGT TCGTCCAAGG CGGCGGTCAA TCACCTCACC CGCAACATTG CCTTCGATGT CGGCCCAATG GGCATTCGCG TGAATGCCAT CGCACCCGGC GCGATCAAGA CCGATGCGCT CGCAACCGTT CTGACGCCTG AAATCGAACG CGCCATGCTC AAGCACACGC CGCTTGGCCG CCTCGGCGAA GCGCAGGATA TCGCCAATGC GGCGCTGTTC CTCTGTTCGC CCGCCGCAGC ATGGATCAGC GGTCAGGTTC TCACGGTTTC CGGCGGCGGC GTGCAGGAAC TCGAC
Details for BrabA.00010.e.A1.GE25556
| HARVESTED ON: | 9/10/2008 |
| SEQUENCED ON: | 7/28/2010 |
| EXPECTED MW: | 28kDa |
| OBSERVED MW: | 28kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 96 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSYESPFHL NDAVAIVTGA AAGIGRAIAG TFAKAGASVV VTDLKSEGAE AVAAAIRQAG GKAIGLECNV TDEQHREAVI KAALDQFGKI TVLVNNAGGG GPKPFDMPMS DFEWAFKLNL FSLFRLSQLA APHMQKACGG AILNISSMAG ENTNVRMASY GSSKAAVNHL TRNIAFDVGP MGIRVNAIAP GAIKTDALAT VLTPEIERAM LKHTPLGRLG EXXDIXXAAL FLCSPAAAWX XGXXLTVSGG GVQXLX
Validated NT Sequence
ttaactttaa gaaggagana taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg tcctatgaga gcccgttcca cctgaatgat gctgttgcaa tcgtcaccgg agcggcggca ggtatcggac gggccatcgc cggaaccttc gccaaggctg gtgcttcggt tgtcgtaacc gaccttaaaa gcgaaggcgc tgaagccgtt gcagccgcaa taaggcaagc tggcggcaag gccatcgggc ttgaatgcaa cgtaaccgac gagcagcatc gcgaagccgt catcaaagcg gccctcgatc agttcggcaa aatcactgtt ctggtcaaca atgcgggtgg aggcggcccc aagcccttcg atatgccgat gagcgacttt gaatgggcct tcaagctcaa tctcttttcc ctgtttcgcc tgtcgcaatt ggctgcccca cacatgcaga aggcttgcgg cggcgcaatt ctcaatattt cgtccatggc cggagaaaat accaatgtcc ggatggcctc ctatggttcg tccaaggcgg cggtcaatca cctcacccgc aacattgcct tcgatgtcgg cccaatgggc attcgcgtga atgccatcgc acccggcgcg atcaagaccg atgcgctcgc aaccgttctg acgcctgaaa tcgaacgcgc catgctcaag cacacgccgc ttggccgcct cggcgaancg cangatatcn ccnatgcggc gctgttcctc tgttcgcccg ccgcagcatg gnncancggt cnggntctca cggtttccgg cggcggcgtg cagnaactcn actaataaan agcacgaaca agttctgcag ccnancttct cgaggatncn gctgctaaca aagcccgaaa gnnanctgag tnnnctgctg cnncgctgan nanantanca nnaccccnnn gnnnnnaann gnnnnnnngg gtttt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSYESPFHL NDAVAIVTGA AAGIGRAIAG TFAKAGASVV VTDLKSEGAE AVAAAIRQAG GKAIGLECNV TDEQHREAVI KAALDQFGKI TVLVNNAGGG GPKPFDMPMS DFEWAFKLNL FSLFRLSQLA APHMQKAGGG AILNISSMAG ENTNVRMASY GSSKAAVNHL TRNIAFDVGP MGIRVNAIAP GAIKTDALAT VLTPEIERAM LKHTPLGRLG EAQDIANAAL FLCSPAAAWI SGQVLTVSGG GVQELD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcctatgag agcccgttcc acctgaatga tgctgttgca atcgtcaccg gagcggcggc aggtatcgga cgggccatcg ccggaacctt cgccaaggct ggtgcttcgg ttgtcgtaac cgaccttaaa agcgaaggcg ctgaagccgt tgcagccgca ataaggcaag ctggcggcaa ggccatcggg cttgaatgca acgtaaccga cgagcagcat cgcgaagccg tcatcaaagc ggccctcgat cagttcggca aaatcactgt tctggtcaac aatgcgggtg gaggcggccc caagcccttc gatatgccga tgagcgactt tgaatgggcc ttcaagctca atctcttttc cctgtttcgc ctgtcgcaat tggctgcccc acacatgcag aaggctggcg gcggcgcaat tctcaatatt tcgtccatgg ccggagaaaa taccaatgtc cggatggcct cctatggttc gtccaaggcg gcggtcaatc acctcacccg caacattgcc ttcgatgtcg gcccaatggg cattcgcgtg aatgccatcg cacccggcgc gatcaagacc gatgcgctcg caaccgttct gacgcctgaa atcgaacgcg ccatgctcaa gcacacgccg cttggccgcc tcggcgaagc gcaggatatc gccaatgcgg cgctgttcct ctgttcgccc gccgcagcat ggatcagcgg tcaggttctc acggtttccg gcggcggcgt gcaggaactc gacaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa