BrabA.00010.d
Short-chain dehydrogenase/reductase SDR:Glucose/ribitol dehydrogenase
| CENTER ID: | BrabA.00010.d |
| ORGANISM: | Brucella melitensis biovar Abortus 2308 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.00010.d.A1.GE25555 | Full length( BrabA.00010.d ) | 1 | 245 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.00010.d.A1.PS00356 | Full length( BrabA.00010.d ) | 1 | 245 | ||
| BrabA.00010.d.A1.PW25600 | Full length( BrabA.00010.d ) | 1 | 245 |
Structures
5T5Q
DEPOSITED: 8/31/2016
DETERMINATION: XRay
CLONE: BrabA.00010.d.A1.GE25555
PROTEIN: BrabA.00010.d.A1.PS00356
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_128170 |
| PATRIC ID: | fig|359391.4.peg.2306 |
| RefSeq: | YP_418276.1 |
| UniProt: | Q2YL80 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKFEFENRTL VLTGANGGIG RAIAELFHAS GANLVLTDLD REGLDAFAAS LGSPERIATI KADASSADDA EKTVALAMER FGGIDFLVPS AGIYQAKPFA EMSDADWHRT ISINLDGVFY LCKRALPALK EDSSIVTLAS LAAYRGAYVN AHYGATKGAM VSMTRALSRE LAPKTRVNGV SPGIIETPMT SELLKTRMDE TMTQTPLKRL GKPSEIASVI AFLCSPAASF VTGETIQVNG GIYMA
NT Sequence
ATGAAATTCG AGTTTGAAAA CCGGACGCTC GTTCTCACTG GCGCAAATGG CGGCATTGGC CGCGCGATTG CCGAACTGTT CCATGCTTCC GGCGCCAATC TGGTTCTGAC TGATCTGGAC AGGGAAGGTC TCGACGCTTT CGCCGCATCG CTGGGCAGCC CGGAACGAAT TGCCACCATC AAGGCCGATG CGTCAAGCGC GGACGACGCC GAAAAGACCG TAGCGCTTGC CATGGAGCGT TTCGGCGGCA TCGATTTCCT TGTGCCATCG GCGGGTATCT ATCAGGCGAA ACCCTTCGCC GAAATGTCGG ATGCCGACTG GCACCGCACG ATTTCGATCA ATCTGGATGG CGTCTTCTAT CTGTGCAAGC GCGCTTTGCC CGCCTTGAAG GAGGATTCCT CCATCGTCAC GCTGGCTTCG CTCGCGGCCT ATCGCGGCGC TTATGTGAAC GCGCATTACG GCGCGACCAA GGGCGCCATG GTTTCCATGA CCCGTGCGCT CTCGCGTGAG CTTGCGCCGA AAACCCGCGT CAATGGCGTT TCCCCCGGCA TCATCGAAAC CCCGATGACC AGCGAGCTTC TCAAGACGCG CATGGATGAA ACCATGACAC AGACGCCGCT GAAGCGGCTT GGCAAACCAT CCGAAATTGC ATCCGTCATT GCCTTCCTCT GTTCACCTGC CGCGAGCTTC GTCACGGGCG AAACGATCCA GGTGAATGGC GGCATCTACA TGGCC
Details for BrabA.00010.d.A1.GE25555
| HARVESTED ON: | 9/10/2008 |
| SEQUENCED ON: | 3/16/2012 |
| EXPECTED MW: | 28kDa |
| OBSERVED MW: | 28kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKFEFENRT LVLTGANGGI GRAIAELFHA SGANLVLTDL DREGLDAFAA SLGSPERIAT IKADASSADD AEKTVALAME RFGGIDFLVP SAGIYQAKPF AEMSDADWHR TISINLDGVF YLCKRALPAL KEDSSIVTLA SLAAYRGAYV NAHYGATKGA MVSMTRALSR ELAPKTRVNG VSPGIIETPM TSELLKTRMD ETMTQTPLKR LGKPSEIASV IAFLCSPAAS FVTGETIQVN GGIYMA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaat tcgagtttga aaaccggacg ctcgttctca ctggcgcaaa tggcggcatt ggccgcgcga ttgccgaact gttccatgct tccggcgcca atctggttct gactgatctg gacagggaag gtctcgacgc tttcgccgca tcgctgggca gcccggaacg aattgccacc atcaaggccg atgcgtcaag cgcggacgac gccgaaaaga ccgtagcgct tgccatggag cgtttcggcg gcatcgattt ccttgtgcca tcggcgggta tctatcaggc gaaacccttc gccgaaatgt cggatgccga ctggcaccgc acgatttcga tcaatctgga tggcgtcttc tatctgtgca agcgcgcttt gcccgccttg aaggaggatt cctccatcgt cacgctggct tcgctcgcgg cctatcgcgg cgcttatgtg aacgcgcatt acggcgcgac caagggcgcc atggtttcca tgacccgtgc gctctcgcgt gagcttgcgc cgaaaacccg cgtcaatggc gtttcccccg gcatcatcga aaccccgatg accagcgagc ttctcaagac gcgcatggat gaaaccatga cacagacgcc gctgaagcgg cttggcaaac catccgaaat tgcatccgtc attgccttcc tctgttcacc tgccgcgagc ttcgtcacgg gcgaaacgat ccaggtgaat ggcggcatct acatggccta ataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKFEFENRT LVLTGANGGI GRAIAELFHA SGANLVLTDL DREGLDAFAA SLGSPERIAT IKADASSADD AEKTVALAME RFGGIDFLVP SAGIYQAKPF AEMSDADWHR TISINLDGVF YLCKRALPAL KEDSSIVTLA SLAAYRGAYV NAHYGATKGA MVSMTRALSR ELAPKTRVNG VSPGIIETPM TSELLKTRMD ETMTQTPLKR LGKPSEIASV IAFLCSPAAS FVTGETIQVN GGIYMA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaattcgag tttgaaaacc ggacgctcgt tctcactggc gcaaatggcg gcattggccg cgcgattgcc gaactgttcc atgcttccgg cgccaatctg gttctgactg atctggacag ggaaggtctc gacgctttcg ccgcatcgct gggcagcccg gaacgaattg ccaccatcaa ggccgatgcg tcaagcgcgg acgacgccga aaagaccgta gcgcttgcca tggagcgttt cggcggcatc gatttccttg tgccatcggc gggtatctat caggcgaaac ccttcgccga aatgtcggat gccgactggc accgcacgat ttcgatcaat ctggatggcg tcttctatct gtgcaagcgc gctttgcccg ccttgaagga ggattcctcc atcgtcacgc tggcttcgct cgcggcctat cgcggcgctt atgtgaacgc gcattacggc gcgaccaagg gcgccatggt ttccatgacc cgtgcgctct cgcgtgagct tgcgccgaaa acccgcgtca atggcgtttc ccccggcatc atcgaaaccc cgatgaccag cgagcttctc aagacgcgca tggatgaaac catgacacag acgccgctga agcggcttgg caaaccatcc gaaattgcat ccgtcattgc cttcctctgt tcacctgccg cgagcttcgt cacgggcgaa acgatccagg tgaatggcgg catctacatg gccaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for BrabA.00010.d.A1.PS00356
| PURIFICATION DATe: | 9/11/2009 |
| CONCENTRATION: | 65.2mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 8 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKFEFENRT LVLTGANGGI GRAIAELFHA SGANLVLTDL DREGLDAFAA SLGSPERIAT IKADASSADD AEKTVALAME RFGGIDFLVP SAGIYQAKPF AEMSDADWHR TISINLDGVF YLCKRALPAL KEDSSIVTLA SLAAYRGAYV NAHYGATKGA MVSMTRALSR ELAPKTRVNG VSPGIIETPM TSELLKTRMD ETMTQTPLKR LGKPSEIASV IAFLCSPAAS FVTGETIQVN GGIYMA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaat tcgagtttga aaaccggacg ctcgttctca ctggcgcaaa tggcggcatt ggccgcgcga ttgccgaact gttccatgct tccggcgcca atctggttct gactgatctg gacagggaag gtctcgacgc tttcgccgca tcgctgggca gcccggaacg aattgccacc atcaaggccg atgcgtcaag cgcggacgac gccgaaaaga ccgtagcgct tgccatggag cgtttcggcg gcatcgattt ccttgtgcca tcggcgggta tctatcaggc gaaacccttc gccgaaatgt cggatgccga ctggcaccgc acgatttcga tcaatctgga tggcgtcttc tatctgtgca agcgcgcttt gcccgccttg aaggaggatt cctccatcgt cacgctggct tcgctcgcgg cctatcgcgg cgcttatgtg aacgcgcatt acggcgcgac caagggcgcc atggtttcca tgacccgtgc gctctcgcgt gagcttgcgc cgaaaacccg cgtcaatggc gtttcccccg gcatcatcga aaccccgatg accagcgagc ttctcaagac gcgcatggat gaaaccatga cacagacgcc gctgaagcgg cttggcaaac catccgaaat tgcatccgtc attgccttcc tctgttcacc tgccgcgagc ttcgtcacgg gcgaaacgat ccaggtgaat ggcggcatct acatggccta ataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKFEFENRT LVLTGANGGI GRAIAELFHA SGANLVLTDL DREGLDAFAA SLGSPERIAT IKADASSADD AEKTVALAME RFGGIDFLVP SAGIYQAKPF AEMSDADWHR TISINLDGVF YLCKRALPAL KEDSSIVTLA SLAAYRGAYV NAHYGATKGA MVSMTRALSR ELAPKTRVNG VSPGIIETPM TSELLKTRMD ETMTQTPLKR LGKPSEIASV IAFLCSPAAS FVTGETIQVN GGIYMA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAATTCGAG TTTGAAAACC GGACGCTCGT TCTCACTGGC GCAAATGGCG GCATTGGCCG CGCGATTGCC GAACTGTTCC ATGCTTCCGG CGCCAATCTG GTTCTGACTG ATCTGGACAG GGAAGGTCTC GACGCTTTCG CCGCATCGCT GGGCAGCCCG GAACGAATTG CCACCATCAA GGCCGATGCG TCAAGCGCGG ACGACGCCGA AAAGACCGTA GCGCTTGCCA TGGAGCGTTT CGGCGGCATC GATTTCCTTG TGCCATCGGC GGGTATCTAT CAGGCGAAAC CCTTCGCCGA AATGTCGGAT GCCGACTGGC ACCGCACGAT TTCGATCAAT CTGGATGGCG TCTTCTATCT GTGCAAGCGC GCTTTGCCCG CCTTGAAGGA GGATTCCTCC ATCGTCACGC TGGCTTCGCT CGCGGCCTAT CGCGGCGCTT ATGTGAACGC GCATTACGGC GCGACCAAGG GCGCCATGGT TTCCATGACC CGTGCGCTCT CGCGTGAGCT TGCGCCGAAA ACCCGCGTCA ATGGCGTTTC CCCCGGCATC ATCGAAACCC CGATGACCAG CGAGCTTCTC AAGACGCGCA TGGATGAAAC CATGACACAG ACGCCGCTGA AGCGGCTTGG CAAACCATCC GAAATTGCAT CCGTCATTGC CTTCCTCTGT TCACCTGCCG CGAGCTTCGT CACGGGCGAA ACGATCCAGG TGAATGGCGG CATCTACATG GCCAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA
Details for BrabA.00010.d.A1.PW25600
| PURIFICATION DATe: | 9/22/2008 |
| CONCENTRATION: | 18.3mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 18 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKFEFENRT LVLTGANGGI GRAIAELFHA SGANLVLTDL DREGLDAFAA SLGSPERIAT IKADASSADD AEKTVALAME RFGGIDFLVP SAGIYQAKPF AEMSDADWHR TISINLDGVF YLCKRALPAL KEDSSIVTLA SLAAYRGAYV NAHYGATKGA MVSMTRALSR ELAPKTRVNG VSPGIIETPM TSELLKTRMD ETMTQTPLKR LGKPSEIASV IAFLCSPAAS FVTGETIQVN GGIYMA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaat tcgagtttga aaaccggacg ctcgttctca ctggcgcaaa tggcggcatt ggccgcgcga ttgccgaact gttccatgct tccggcgcca atctggttct gactgatctg gacagggaag gtctcgacgc tttcgccgca tcgctgggca gcccggaacg aattgccacc atcaaggccg atgcgtcaag cgcggacgac gccgaaaaga ccgtagcgct tgccatggag cgtttcggcg gcatcgattt ccttgtgcca tcggcgggta tctatcaggc gaaacccttc gccgaaatgt cggatgccga ctggcaccgc acgatttcga tcaatctgga tggcgtcttc tatctgtgca agcgcgcttt gcccgccttg aaggaggatt cctccatcgt cacgctggct tcgctcgcgg cctatcgcgg cgcttatgtg aacgcgcatt acggcgcgac caagggcgcc atggtttcca tgacccgtgc gctctcgcgt gagcttgcgc cgaaaacccg cgtcaatggc gtttcccccg gcatcatcga aaccccgatg accagcgagc ttctcaagac gcgcatggat gaaaccatga cacagacgcc gctgaagcgg cttggcaaac catccgaaat tgcatccgtc attgccttcc tctgttcacc tgccgcgagc ttcgtcacgg gcgaaacgat ccaggtgaat ggcggcatct acatggccta ataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKFEFENRT LVLTGANGGI GRAIAELFHA SGANLVLTDL DREGLDAFAA SLGSPERIAT IKADASSADD AEKTVALAME RFGGIDFLVP SAGIYQAKPF AEMSDADWHR TISINLDGVF YLCKRALPAL KEDSSIVTLA SLAAYRGAYV NAHYGATKGA MVSMTRALSR ELAPKTRVNG VSPGIIETPM TSELLKTRMD ETMTQTPLKR LGKPSEIASV IAFLCSPAAS FVTGETIQVN GGIYMA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAATTCGAG TTTGAAAACC GGACGCTCGT TCTCACTGGC GCAAATGGCG GCATTGGCCG CGCGATTGCC GAACTGTTCC ATGCTTCCGG CGCCAATCTG GTTCTGACTG ATCTGGACAG GGAAGGTCTC GACGCTTTCG CCGCATCGCT GGGCAGCCCG GAACGAATTG CCACCATCAA GGCCGATGCG TCAAGCGCGG ACGACGCCGA AAAGACCGTA GCGCTTGCCA TGGAGCGTTT CGGCGGCATC GATTTCCTTG TGCCATCGGC GGGTATCTAT CAGGCGAAAC CCTTCGCCGA AATGTCGGAT GCCGACTGGC ACCGCACGAT TTCGATCAAT CTGGATGGCG TCTTCTATCT GTGCAAGCGC GCTTTGCCCG CCTTGAAGGA GGATTCCTCC ATCGTCACGC TGGCTTCGCT CGCGGCCTAT CGCGGCGCTT ATGTGAACGC GCATTACGGC GCGACCAAGG GCGCCATGGT TTCCATGACC CGTGCGCTCT CGCGTGAGCT TGCGCCGAAA ACCCGCGTCA ATGGCGTTTC CCCCGGCATC ATCGAAACCC CGATGACCAG CGAGCTTCTC AAGACGCGCA TGGATGAAAC CATGACACAG ACGCCGCTGA AGCGGCTTGG CAAACCATCC GAAATTGCAT CCGTCATTGC CTTCCTCTGT TCACCTGCCG CGAGCTTCGT CACGGGCGAA ACGATCCAGG TGAATGGCGG CATCTACATG GCCAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA