BrabA.00010.c
possible enoyl-(acyl-carrier-protein) reductase
| CENTER ID: | BrabA.00010.c |
| ORGANISM: | Brucella melitensis biovar Abortus 2308 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.00010.c.A1.GE25365 | Full length( BrabA.00010.c ) | 1 | 272 | ||
| BrabA.00010.c.A1.GE25365 | Full length( BrabA.00010.c ) | 1 | 272 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.00010.c.A1.PS00047 | Full length( BrabA.00010.c ) | 1 | 272 |
Structures
5KOI
DEPOSITED: 6/30/2016
DETERMINATION: XRay
CLONE: BrabA.00010.c.A1.GE25365
PROTEIN: BrabA.00010.c.A1.PS00047
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_130466 |
| PATRIC ID: | fig|359391.4.peg.2261 |
| RefSeq: | YP_415492 |
| UniProt: | Q2YQR0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEGLMQGKRG LIMGVANNHS LAWGIAKQLA AQGAELAFTY QGDALGKRVK PLAEQVGSDF VLPCDVEDIA TVDAVFEEIE KKWGGLDFLV HAIGFSDKTE LKGRYADVTT RENFSRTMVI SAYSFTEVAQ RAEKLMKDGG SILTLTYGGS TRTIPNYNVM GVAKAALEAM VRYLAADYGP QGIRVNAISA GPVRTLAGAG IGDARAIFSY QRRNSPLRRT VDIDDVGKSA VYLLSDLSSG VTGEIHFVDS GYNIVSMPTL EELKSSDSER GE
NT Sequence
ATGGAAGGTT TGATGCAAGG AAAACGCGGC CTCATCATGG GGGTCGCGAA CAATCACTCA CTGGCCTGGG GCATTGCAAA ACAGCTCGCC GCACAGGGCG CCGAACTGGC CTTCACCTAT CAGGGTGATG CGCTGGGCAA GCGCGTGAAA CCTCTTGCCG AACAGGTCGG CTCGGATTTC GTGCTGCCTT GCGACGTGGA GGACATCGCC ACCGTCGATG CCGTTTTCGA AGAAATCGAG AAGAAGTGGG GCGGTCTCGA TTTCCTCGTG CACGCTATCG GTTTTTCCGA CAAGACGGAA TTGAAGGGCC GCTATGCAGA TGTCACCACG CGCGAGAATT TCAGCCGCAC GATGGTCATC TCCGCCTATT CCTTCACAGA AGTGGCGCAG CGCGCTGAAA AGCTCATGAA GGACGGCGGC TCGATCCTGA CGCTGACCTA TGGCGGATCG ACCCGAACGA TCCCCAATTA CAACGTGATG GGTGTTGCCA AGGCCGCTCT CGAGGCCATG GTGCGCTATC TTGCAGCCGA TTACGGCCCG CAGGGCATCC GCGTCAACGC GATTTCCGCA GGCCCCGTGC GCACGCTTGC TGGCGCCGGG ATCGGTGATG CCCGCGCGAT TTTCAGCTAC CAGCGCCGCA ACTCGCCGCT GCGCCGCACC GTCGATATCG ATGATGTCGG CAAATCGGCA GTCTATCTTC TGTCCGATCT TTCAAGCGGT GTGACCGGCG AAATCCATTT CGTTGATTCC GGCTATAACA TCGTTTCGAT GCCGACGCTG GAAGAGCTGA AAAGTTCGGA CTCCGAACGC GGAGAA
Details for BrabA.00010.c.A1.GE25365
| HARVESTED ON: | 6/30/2008 |
| SEQUENCED ON: | 3/16/2012 |
| EXPECTED MW: | 31kDa |
| OBSERVED MW: | 32kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMEGLMQGKR GLIMGVANNH SLAWGIAKQL AAQGAELAFT YQGDALGKRV KPLAEQVGSD FVLPCDVEDI ATVDAVFEEI EKKWGGLDFL VHAIGFSDKT ELKGRYADVT TRENFSRTMV ISAYSFTEVA QRAEKLMKDG GSILTLTYGG STRTIPNYNV MGVAKAALEA MVRYLAADYG PQGIRVNAIS AGPVRTLAGA GIGDARAIFS YQRRNSPLRR TVDIDDVGKS AVYLLSDLSS GVTGEIHFVX SGYNIVSMPT LEELKSSDSE RGXX
Validated NT Sequence
tttaagaagg agnnatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggaagg tttgatgcaa ggaaaacgcg gcctcatcat gggggtcgcg aacaatcact cactggcctg gggcattgca aaacagctcg ccgcacaggg cgccgaactg gccttcacct atcagggtga tgcgctgggc aagcgcgtga aacctcttgc cgaacaggtc ggctcggatt tcgtgctgcc ttgcgacgtg gaggacatcg ccaccgtcga tgccgttttc gaagaaatcg agaagaagtg gggcggtctc gatttcctcg tgcacgctat cggtttttcc gacaagacgg aattgaaggg ccgctatgca gatgtcacca cgcgcgagaa tttcagccgc acgatggtca tctccgccta ttccttcaca gaagtggcgc agcgcgctga aaagctcatg aaggacggcg gctcgatcct gacgctgacc tatggcggat cgacccgaac gatccccaat tacaacgtga tgggtgttgc caaggccgct ctcgaggcca tggtgcgcta tcttgcagcc gattacggcc cgcagggcat ccgcgtcaac gcgatttccg caggccccgt gcgcacgctt gctggcgccg ggatcggtga tgcccgcgcg attttcagct accagcgccg caactcgccg ctgcgccgca ccgtcgatat cgatgatgtc ggcaaatcgg cagtctatct tctgtccgat ctttcaagcg gtgtgaccgg cgaaatccat ttcgttgant ccggctataa catcgtttcg atgccnacgc tggaagagct gaaaagttcg gactccgaac gcgganaata ntaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMEGLMQGKR GLIMGVANNH SLAWGIAKQL AAQGAELAFT YQGDALGKRV KPLAEQVGSD FVLPCDVEDI ATVDAVFEEI EKKWGGLDFL VHAIGFSDKT ELKGRYADVT TRENFSRTMV ISAYSFTEVA QRAEKLMKDG GSILTLTYGG STRTIPNYNV MGVAKAALEA MVRYLAADYG PQGIRVNAIS AGPVRTLAGA GIGDARAIFS YQRRNSPLRR TVDIDDVGKS AVYLLSDLSS GVTGEIHFVD SGYNIVSMPT LEELKSSDSE RGE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaaggtttg atgcaaggaa aacgcggcct catcatgggg gtcgcgaaca atcactcact ggcctggggc attgcaaaac agctcgccgc acagggcgcc gaactggcct tcacctatca gggtgatgcg ctgggcaagc gcgtgaaacc tcttgccgaa caggtcggct cggatttcgt gctgccttgc gacgtggagg acatcgccac cgtcgatgcc gttttcgaag aaatcgagaa gaagtggggc ggtctcgatt tcctcgtgca cgctatcggt ttttccgaca agacggaatt gaagggccgc tatgcagatg tcaccacgcg cgagaatttc agccgcacga tggtcatctc cgcctattcc ttcacagaag tggcgcagcg cgctgaaaag ctcatgaagg acggcggctc gatcctgacg ctgacctatg gcggatcgac ccgaacgatc cccaattaca acgtgatggg tgttgccaag gccgctctcg aggccatggt gcgctatctt gcagccgatt acggcccgca gggcatccgc gtcaacgcga tttccgcagg ccccgtgcgc acgcttgctg gcgccgggat cggtgatgcc cgcgcgattt tcagctacca gcgccgcaac tcgccgctgc gccgcaccgt cgatatcgat gatgtcggca aatcggcagt ctatcttctg tccgatcttt caagcggtgt gaccggcgaa atccatttcg ttgattccgg ctataacatc gtttcgatgc cgacgctgga agagctgaaa agttcggact ccgaacgcgg agaataaaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for BrabA.00010.c.A1.GE25365
| HARVESTED ON: | 6/30/2008 |
| SEQUENCED ON: | 3/16/2012 |
| EXPECTED MW: | 31kDa |
| OBSERVED MW: | 32kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMEGLMQGKR GLIMGVANNH SLAWGIAKQL AAQGAELAFT YQGDALGKRV KPLAEQVGSD FVLPCDVEDI ATVDAVFEEI EKKWGGLDFL VHAIGFSDKT ELKGRYADVT TRENFSRTMV ISAYSFTEVA QRAEKLMKDG GSILTLTYGG STRTIPNYNV MGVAKAALEA MVRYLAADYG PQGIRVNAIS AGPVRTLAGA GIGDARAIFS YQRRNSPLRR TVDIDDVGKS AVYLLSDLSS GVTGEIHFVX SGYNIVSMPT LEELKSSDSE RGXX
Validated NT Sequence
tttaagaagg agnnatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggaagg tttgatgcaa ggaaaacgcg gcctcatcat gggggtcgcg aacaatcact cactggcctg gggcattgca aaacagctcg ccgcacaggg cgccgaactg gccttcacct atcagggtga tgcgctgggc aagcgcgtga aacctcttgc cgaacaggtc ggctcggatt tcgtgctgcc ttgcgacgtg gaggacatcg ccaccgtcga tgccgttttc gaagaaatcg agaagaagtg gggcggtctc gatttcctcg tgcacgctat cggtttttcc gacaagacgg aattgaaggg ccgctatgca gatgtcacca cgcgcgagaa tttcagccgc acgatggtca tctccgccta ttccttcaca gaagtggcgc agcgcgctga aaagctcatg aaggacggcg gctcgatcct gacgctgacc tatggcggat cgacccgaac gatccccaat tacaacgtga tgggtgttgc caaggccgct ctcgaggcca tggtgcgcta tcttgcagcc gattacggcc cgcagggcat ccgcgtcaac gcgatttccg caggccccgt gcgcacgctt gctggcgccg ggatcggtga tgcccgcgcg attttcagct accagcgccg caactcgccg ctgcgccgca ccgtcgatat cgatgatgtc ggcaaatcgg cagtctatct tctgtccgat ctttcaagcg gtgtgaccgg cgaaatccat ttcgttgant ccggctataa catcgtttcg atgccnacgc tggaagagct gaaaagttcg gactccgaac gcgganaata ntaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMEGLMQGKR GLIMGVANNH SLAWGIAKQL AAQGAELAFT YQGDALGKRV KPLAEQVGSD FVLPCDVEDI ATVDAVFEEI EKKWGGLDFL VHAIGFSDKT ELKGRYADVT TRENFSRTMV ISAYSFTEVA QRAEKLMKDG GSILTLTYGG STRTIPNYNV MGVAKAALEA MVRYLAADYG PQGIRVNAIS AGPVRTLAGA GIGDARAIFS YQRRNSPLRR TVDIDDVGKS AVYLLSDLSS GVTGEIHFVD SGYNIVSMPT LEELKSSDSE RGE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaaggtttg atgcaaggaa aacgcggcct catcatgggg gtcgcgaaca atcactcact ggcctggggc attgcaaaac agctcgccgc acagggcgcc gaactggcct tcacctatca gggtgatgcg ctgggcaagc gcgtgaaacc tcttgccgaa caggtcggct cggatttcgt gctgccttgc gacgtggagg acatcgccac cgtcgatgcc gttttcgaag aaatcgagaa gaagtggggc ggtctcgatt tcctcgtgca cgctatcggt ttttccgaca agacggaatt gaagggccgc tatgcagatg tcaccacgcg cgagaatttc agccgcacga tggtcatctc cgcctattcc ttcacagaag tggcgcagcg cgctgaaaag ctcatgaagg acggcggctc gatcctgacg ctgacctatg gcggatcgac ccgaacgatc cccaattaca acgtgatggg tgttgccaag gccgctctcg aggccatggt gcgctatctt gcagccgatt acggcccgca gggcatccgc gtcaacgcga tttccgcagg ccccgtgcgc acgcttgctg gcgccgggat cggtgatgcc cgcgcgattt tcagctacca gcgccgcaac tcgccgctgc gccgcaccgt cgatatcgat gatgtcggca aatcggcagt ctatcttctg tccgatcttt caagcggtgt gaccggcgaa atccatttcg ttgattccgg ctataacatc gtttcgatgc cgacgctgga agagctgaaa agttcggact ccgaacgcgg agaataaaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for BrabA.00010.c.A1.PS00047
| PURIFICATION DATe: | 7/24/2008 |
| CONCENTRATION: | 72.4mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 50µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMEGLMQGKR GLIMGVANNH SLAWGIAKQL AAQGAELAFT YQGDALGKRV KPLAEQVGSD FVLPCDVEDI ATVDAVFEEI EKKWGGLDFL VHAIGFSDKT ELKGRYADVT TRENFSRTMV ISAYSFTEVA QRAEKLMKDG GSILTLTYGG STRTIPNYNV MGVAKAALEA MVRYLAADYG PQGIRVNAIS AGPVRTLAGA GIGDARAIFS YQRRNSPLRR TVDIDDVGKS AVYLLSDLSS GVTGEIHFVX SGYNIVSMPT LEELKSSDSE RGXX
Validated NT Sequence
tttaagaagg agnnatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggaagg tttgatgcaa ggaaaacgcg gcctcatcat gggggtcgcg aacaatcact cactggcctg gggcattgca aaacagctcg ccgcacaggg cgccgaactg gccttcacct atcagggtga tgcgctgggc aagcgcgtga aacctcttgc cgaacaggtc ggctcggatt tcgtgctgcc ttgcgacgtg gaggacatcg ccaccgtcga tgccgttttc gaagaaatcg agaagaagtg gggcggtctc gatttcctcg tgcacgctat cggtttttcc gacaagacgg aattgaaggg ccgctatgca gatgtcacca cgcgcgagaa tttcagccgc acgatggtca tctccgccta ttccttcaca gaagtggcgc agcgcgctga aaagctcatg aaggacggcg gctcgatcct gacgctgacc tatggcggat cgacccgaac gatccccaat tacaacgtga tgggtgttgc caaggccgct ctcgaggcca tggtgcgcta tcttgcagcc gattacggcc cgcagggcat ccgcgtcaac gcgatttccg caggccccgt gcgcacgctt gctggcgccg ggatcggtga tgcccgcgcg attttcagct accagcgccg caactcgccg ctgcgccgca ccgtcgatat cgatgatgtc ggcaaatcgg cagtctatct tctgtccgat ctttcaagcg gtgtgaccgg cgaaatccat ttcgttgant ccggctataa catcgtttcg atgccnacgc tggaagagct gaaaagttcg gactccgaac gcgganaata ntaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMEGLMQGKR GLIMGVANNH SLAWGIAKQL AAQGAELAFT YQGDALGKRV KPLAEQVGSD FVLPCDVEDI ATVDAVFEEI EKKWGGLDFL VHAIGFSDKT ELKGRYADVT TRENFSRTMV ISAYSFTEVA QRAEKLMKDG GSILTLTYGG STRTIPNYNV MGVAKAALEA MVRYLAADYG PQGIRVNAIS AGPVRTLAGA GIGDARAIFS YQRRNSPLRR TVDIDDVGKS AVYLLSDLSS GVTGEIHFVD SGYNIVSMPT LEELKSSDSE RGE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGAAGGTTTG ATGCAAGGAA AACGCGGCCT CATCATGGGG GTCGCGAACA ATCACTCACT GGCCTGGGGC ATTGCAAAAC AGCTCGCCGC ACAGGGCGCC GAACTGGCCT TCACCTATCA GGGTGATGCG CTGGGCAAGC GCGTGAAACC TCTTGCCGAA CAGGTCGGCT CGGATTTCGT GCTGCCTTGC GACGTGGAGG ACATCGCCAC CGTCGATGCC GTTTTCGAAG AAATCGAGAA GAAGTGGGGC GGTCTCGATT TCCTCGTGCA CGCTATCGGT TTTTCCGACA AGACGGAATT GAAGGGCCGC TATGCAGATG TCACCACGCG CGAGAATTTC AGCCGCACGA TGGTCATCTC CGCCTATTCC TTCACAGAAG TGGCGCAGCG CGCTGAAAAG CTCATGAAGG ACGGCGGCTC GATCCTGACG CTGACCTATG GCGGATCGAC CCGAACGATC CCCAATTACA ACGTGATGGG TGTTGCCAAG GCCGCTCTCG AGGCCATGGT GCGCTATCTT GCAGCCGATT ACGGCCCGCA GGGCATCCGC GTCAACGCGA TTTCCGCAGG CCCCGTGCGC ACGCTTGCTG GCGCCGGGAT CGGTGATGCC CGCGCGATTT TCAGCTACCA GCGCCGCAAC TCGCCGCTGC GCCGCACCGT CGATATCGAT GATGTCGGCA AATCGGCAGT CTATCTTCTG TCCGATCTTT CAAGCGGTGT GACCGGCGAA ATCCATTTCG TTGATTCCGG CTATAACATC GTTTCGATGC CGACGCTGGA AGAGCTGAAA AGTTCGGACT CCGAACGCGG AGAATAAAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA