BrabA.00010.b

Short-chain dehydrogenase/reductase SDR:Glucose/ribitol dehydrogenase

CENTER ID: BrabA.00010.b
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: in PDB
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BrabA.00010.b.A1.GE25364 Full length( BrabA.00010.b ) 1 245
BrabA.00010.b.B1.GE25460 Full length( BrabA.00010.b ) 1 245
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Structures

3EMK
DEPOSITED: 9/24/2008
DETERMINATION: XRay
CLONE: BrabA.00010.b.A1.GE25364
PROTEIN: BrabA.00010.b.A1.PS00049
3ENN
DEPOSITED: 9/25/2008
DETERMINATION: XRay
CLONE: BrabA.00010.b.A1.GE25364
PROTEIN: BrabA.00010.b.A1.PS00049

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_126618
PATRIC ID: fig|359391.4.peg.504
RefSeq: YP_413943
UniProt: Q2YMG6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFDLTGRKAL VTGATGGLGE AIARALHAQG AIVGLHGTRE EKLKELAAEL GERIFVFPAN LSDREAVKAL GQKAEEEMGG VDILVNNAGI TRDGLFVRMS DEDWDAVLTV NLTSVFNLTR ELTHPMMRRR NGRIINITSI VGVTGNPGQA NYCASKAGLI GFSKSLAQEI ASRNVTVNCI APGFIESAMT GKLNEKQKDA IMGNIPMKRM GVGADIAAAV VYLASDEAAY VTGQTLHVNG GMAMI
NT Sequence
ATGTTTGATC TGACTGGCCG CAAGGCTCTG GTGACCGGCG CAACCGGCGG TCTTGGTGAG GCGATTGCCC GCGCGCTGCA CGCGCAGGGC GCAATTGTCG GCCTGCACGG TACGCGCGAG GAAAAGCTGA AGGAATTGGC TGCGGAGCTG GGTGAACGCA TTTTCGTCTT CCCGGCCAAT CTGTCCGACC GTGAAGCCGT CAAGGCGCTT GGCCAGAAGG CCGAAGAGGA AATGGGTGGT GTCGATATTC TGGTCAACAA TGCGGGCATC ACCCGTGATG GCCTTTTCGT GCGCATGAGC GACGAGGATT GGGATGCGGT TCTGACCGTC AATCTGACGT CGGTATTCAA TCTCACGCGC GAACTGACCC ATCCCATGAT GCGCCGCCGC AACGGCCGCA TCATCAACAT CACCTCCATT GTCGGTGTGA CGGGCAATCC GGGTCAGGCA AATTATTGCG CCTCGAAGGC TGGCCTCATC GGCTTCTCCA AGTCGCTGGC GCAAGAGATC GCAAGCCGCA ACGTGACCGT CAATTGCATC GCGCCGGGCT TTATCGAATC GGCCATGACC GGCAAGCTGA ATGAGAAGCA GAAGGACGCG ATCATGGGCA ATATTCCCAT GAAGCGCATG GGGGTCGGCG CCGATATTGC TGCTGCCGTG GTCTATCTTG CAAGCGACGA GGCGGCCTAT GTGACCGGCC AGACCTTGCA TGTAAATGGC GGCATGGCCA TGATT
Details for BrabA.00010.b.A1.GE25364
HARVESTED ON: 6/30/2008
SEQUENCED ON: 6/16/2009
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMFDLTGRKA LVTGATGGLG EAIARALHAQ GAIVGLHGTR EEKLKELAAE LGERIFVFPA NLSDREAVKA LGQKAEEEMG GVDILVNNAG ITRDGLFVRM SDEDWDAVLT VNLTSVFNLT RELTHPMMRR RNGRIINITS IVGVTGNPGQ ANYCASKAGL IGFSKSLAQE IASRNVTVNC IAPGFIESAM TGKLNEKQKD AIMGNIPMKR MGVGAXXAAA VVYLASDXAA YVTGXXLHVN GGMAMI
Validated NT Sequence
ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtttgatctg actggccgca aggctctggt gaccggcgca accggcggtc ttggtgaggc gattgcccgc gcgctgcacg cgcagggcgc aattgtcggc ctgcacggta cgcgcgagga aaagctgaag gaattggctg cggagctggg tgaacgcatt ttcgtcttcc cggccaatct gtccgaccgt gaagccgtca aggcgcttgg ccagaaggcc gaagaggaaa tgggtggtgt cgatattctg gtcaacaatg cgggcatcac ccgtgatggc cttttcgtgc gcatgagcga cgaggattgg gatgcggttc tgaccgtcaa tctgacgtcg gtattcaatc tcacgcgcga actgacccat cccatgatgc gccgccgcaa cggccgcatc atcaacatca cctccattgt cggtgtgacg ggcaatccgg gtcaggcaaa ttattgcgcc tcgaaggctg gcctcatcgg cttctccaag tcgctggcgc aagagatcgc aagccgcaac gtgaccgtca attgcatcgc gccgggcttt atcgaatcgg ccatgaccgg caagctgaat gagaagcaga aggacgcgat catgggcaat attcccatga agcgcatggg ggtcggcgcc natnttgctg ctgccgtggt ctatcttgca agcgacnang cggcctatgt gaccggccng ancttgcatg taaatggcgg catggccatg atttaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFDLTGRKA LVTGATGGLG EAIARALHAQ GAIVGLHGTR EEKLKELAAE LGERIFVFPA NLSDREAVKA LGQKAEEEMG GVDILVNNAG ITRDGLFVRM SDEDWDAVLT VNLTSVFNLT RELTHPMMRR RNGRIINITS IVGVTGNPGQ ANYCASKAGL IGFSKSLAQE IASRNVTVNC IAPGFIESAM TGKLNEKQKD AIMGNIPMKR MGVGADIAAA VVYLASDEAA YVTGQTLHVN GGMAMI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtttgatctg actggccgca aggctctggt gaccggcgca accggcggtc ttggtgaggc gattgcccgc gcgctgcacg cgcagggcgc aattgtcggc ctgcacggta cgcgcgagga aaagctgaag gaattggctg cggagctggg tgaacgcatt ttcgtcttcc cggccaatct gtccgaccgt gaagccgtca aggcgcttgg ccagaaggcc gaagaggaaa tgggtggtgt cgatattctg gtcaacaatg cgggcatcac ccgtgatggc cttttcgtgc gcatgagcga cgaggattgg gatgcggttc tgaccgtcaa tctgacgtcg gtattcaatc tcacgcgcga actgacccat cccatgatgc gccgccgcaa cggccgcatc atcaacatca cctccattgt cggtgtgacg ggcaatccgg gtcaggcaaa ttattgcgcc tcgaaggctg gcctcatcgg cttctccaag tcgctggcgc aagagatcgc aagccgcaac gtgaccgtca attgcatcgc gccgggcttt atcgaatcgg ccatgaccgg caagctgaat gagaagcaga aggacgcgat catgggcaat attcccatga agcgcatggg ggtcggcgcc gatattgctg ctgccgtggt ctatcttgca agcgacgagg cggcctatgt gaccggccag accttgcatg taaatggcgg catggccatg atttgaaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BrabA.00010.b.B1.GE25460
HARVESTED ON: 7/11/2008
SEQUENCED ON: 3/6/2009
EXPECTED MW: 26kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMF DLTGRKALVT GATGGLGEAI ARALHAQGAI VGLHGTREEK LKELAAELGE RIFVFPANLS DREAVKALGQ KAEEEMGGVD ILVNNAGITR DGLFVRMSDE DWDAVLTVNL TSVFNLTREL THPMMRRRNG RIINITSIVG VTGNPGQANY CASKAGLIGF SKSLAQEIAS RNVTVNCIAP GFIESAMTGK LNEKQKDAIM GNIPMKRMGV GADIAAAVVY LASDEAAYVT GQTLHVNGGM AMIK
Validated NT Sequence
tttaagaagg agnnntacca tggctcacca ccaccaccac catatgtttg atctgactgg ccgcaaggct ctggtgaccg gcgcaaccgg cggtcttggt gaggcgattg cccgcgcgct gcacgcgcag ggcgcaattg tcggcctgca cggtacgcgc gaggaaaagc tgaaggaatt ggctgcggag ctgggtgaac gcattttcgt cttcccggcc aatctgtccg accgtgaagc cgtcaaggcg cttggccaga aggccgaaga ggaaatgggt ggtgtcgata ttctggtcaa caatgcgggc atcacccgtg atggcctttt cgtgcgcatg agcgacgagg attgggatgc ggttctgacc gtcaatctga cgtcggtatt caatctcacg cgcgaactga cccatcccat gatgcgccgc cgcaacggcc gcatcatcaa catcacctcc attgtcggtg tgacgggcaa tccgggtcag gcaaattatt gcgcctcgaa ggctggcctc atcggcttct ccaagtcgct ggcgcaagag atcgcaagcc gcaacgtgac cgtcaattgc atcgcgccgg gctttatcga atcggccatg accggcaagc tgaatgagaa gcagaaggac gcgatcatgg gcaatattcc catgaagcgc atgggggtcg gcgccgatat tgctgctgcc gtggtctatc ttgcaagcga cgaggcggcc tatgtgaccg gccagacctt gcatgtaaat ggcggcatgg ccatgattaa gtgagtaaga t
Expected Protein Sequence
MAHHHHHHMF DLTGRKALVT GATGGLGEAI ARALHAQGAI VGLHGTREEK LKELAAELGE RIFVFPANLS DREAVKALGQ KAEEEMGGVD ILVNNAGITR DGLFVRMSDE DWDAVLTVNL TSVFNLTREL THPMMRRRNG RIINITSIVG VTGNPGQANY CASKAGLIGF SKSLAQEIAS RNVTVNCIAP GFIESAMTGK LNEKQKDAIM GNIPMKRMGV GADIAAAVVY LASDEAAYVT GQTLHVNGGM AMI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtttg atctgactgg ccgcaaggct ctggtgaccg gcgcaaccgg cggtcttggt gaggcgattg cccgcgcgct gcacgcgcag ggcgcaattg tcggcctgca cggtacgcgc gaggaaaagc tgaaggaatt ggctgcggag ctgggtgaac gcattttcgt cttcccggcc aatctgtccg accgtgaagc cgtcaaggcg cttggccaga aggccgaaga ggaaatgggt ggtgtcgata ttctggtcaa caatgcgggc atcacccgtg atggcctttt cgtgcgcatg agcgacgagg attgggatgc ggttctgacc gtcaatctga cgtcggtatt caatctcacg cgcgaactga cccatcccat gatgcgccgc cgcaacggcc gcatcatcaa catcacctcc attgtcggtg tgacgggcaa tccgggtcag gcaaattatt gcgcctcgaa ggctggcctc atcggcttct ccaagtcgct ggcgcaagag atcgcaagcc gcaacgtgac cgtcaattgc atcgcgccgg gctttatcga atcggccatg accggcaagc tgaatgagaa gcagaaggac gcgatcatgg gcaatattcc catgaagcgc atgggggtcg gcgccgatat tgctgctgcc gtggtctatc ttgcaagcga cgaggcggcc tatgtgaccg gccagacctt gcatgtaaat ggcggcatgg ccatgatttg atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat