BrabA.00010.a

Short-chain dehydrogenase/reductase SDR:Glucose/ribitol dehydrogenase

CENTER ID: BrabA.00010.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: in PDB
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BrabA.00010.a.A1.GE25363 Full length( BrabA.00010.a ) 1 272
BrabA.00010.a.B1.GE25459 Full length( BrabA.00010.a ) 1 272
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BrabA.00010.a.A1.PS00048 Full length( BrabA.00010.a ) 1 272

Structures

3GRK
DEPOSITED: 3/25/2009
DETERMINATION: XRay
CLONE: BrabA.00010.a.A1.GE25363
PROTEIN: BrabA.00010.a.A1.PS00048

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_130466
PATRIC ID: fig|359391.4.peg.468
RefSeq: YP_413907
UniProt: Q2YMC8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTAQSGLLQG KRGLILGVAN NRSIAWGIAK AAREAGAELA FTYQGDALKK RVEPLAEELG AFVAGHCDVA DAASIDAVFE TLEKKWGKLD FLVHAIGFSD KDELTGRYID TSEANFTNTM LISVYSLTAV SRRAEKLMAD GGSILTLTYY GAEKVMPNYN VMGVAKAALE ASVKYLAVDL GPQNIRVNAI SAGPIKTLAA SGIGDFRYIL KWNEYNAPLR RTVTIDEVGD VGLYFLSDLS RSVTGEVHHA DSGYHVIGMK AVDAPDISVV KD
NT Sequence
ATGACCGCAC AGTCTGGTTT GTTGCAGGGC AAGCGTGGAT TGATTCTAGG CGTGGCCAAT AATCGCTCTA TCGCTTGGGG CATTGCAAAA GCCGCCCGTG AAGCTGGCGC AGAGCTTGCA TTCACCTATC AGGGTGACGC ATTGAAGAAG CGTGTGGAGC CGCTGGCCGA GGAGCTTGGC GCCTTCGTCG CCGGACATTG CGACGTCGCC GATGCGGCGA GCATCGATGC GGTTTTTGAA ACGCTTGAGA AGAAGTGGGG CAAGCTCGAC TTCCTGGTGC ACGCAATCGG TTTCTCCGAC AAGGACGAGC TGACCGGCCG CTATATCGAT ACGTCGGAAG CCAATTTCAC CAATACGATG CTGATTTCGG TCTATTCGCT GACGGCGGTT TCGCGCCGCG CGGAAAAGCT GATGGCCGAT GGCGGATCGA TTCTGACGCT GACCTATTAC GGCGCTGAAA AGGTCATGCC GAATTACAAT GTCATGGGCG TGGCCAAGGC AGCCCTTGAG GCGAGCGTGA AATATCTGGC CGTCGATCTT GGCCCCCAGA ACATTCGCGT CAATGCAATC TCGGCTGGCC CCATCAAGAC GCTTGCCGCT TCCGGCATTG GTGACTTCCG CTATATCCTG AAGTGGAACG AATATAATGC GCCGCTGCGC CGCACCGTCA CGATCGACGA GGTGGGTGAT GTCGGCCTTT ATTTCCTTTC GGACCTGTCG CGTTCGGTTA CAGGCGAAGT GCACCATGCC GACAGCGGCT ATCATGTCAT TGGCATGAAA GCGGTTGACG CACCGGATAT CTCGGTCGTG AAAGAC
Details for BrabA.00010.a.A1.GE25363
HARVESTED ON: 6/30/2008
SEQUENCED ON: 6/16/2009
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 95
PERCENT COVERAGE: 96
Validated AA Sequence
MGTLEAQTQG PGSMTAQSGL LQGKRGLILG VANNRSIAWG IAKAAREAGA ELAFTYQGDA LKKRVEPLAE ELGAFVAGHC DVADAASIDA VFETLEKKWG KLDFLVHAIG FSDKDELTGR YIDTSEANFT NTMLISVYSL TAVSRRAEKL MADGGSILTL TYYGAEKVMP NYNVMGVAKA ALEASVKYLA VDLGPQNIRV NAISAGPIKT LAASGIXDFR YILKXNEYNA PLRXXVTIDX VGDVGLYFLS XXSRSVXGEV HHADSXYHXI GXKXVXAPDX SVV
Validated NT Sequence
tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgaccgc acagtctggt ttgttgcagg gcaagcgtgg attgattcta ggcgtggcca ataatcgctc tatcgcttgg ggcattgcaa aagccgcccg tgaagctggc gcagagcttg cattcaccta tcagggtgac gcattgaaga agcgtgtgga gccgctggcc gaggagcttg gcgccttcgt cgccggacat tgcgacgtcg ccgatgcggc gagcatcgat gcggtttttg aaacgcttga gaagaagtgg ggcaagctcg acttcctggt gcacgcaatc ggtttctccg acaaggacga gctgaccggc cgctatatcg atacgtcgga agccaatttc accaatacga tgctgatttc ggtctattcg ctgacggcgg tttcgcgccg cgcggaaaag ctgatggccg atggcggatc gattctgacg ctgacctatt acggcgctga aaaggtcatg ccgaattaca atgtcatggg cgtggccaag gcagcccttg aggcgagcgt gaaatatctg gccgtcgatc ttggccccca gaacattcgc gtcaatgcaa tctcggctgg ccccatcaag acgcttgccg cttccggcat tgntgacttc cgctatatcc tgaagtgnaa cgaatataat gcgccgctgc gccncancgt cacgatcgac gangtgggtg atgtcggcct ttatttcctt tcgnanntgt cgcgttcggt tncnggcgaa gtgcaccatg ccgacagcng ctatcatgnc attggnnnga aancggttga ngcaccggat nnctcggtcg tga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTAQSGLLQ GKRGLILGVA NNRSIAWGIA KAAREAGAEL AFTYQGDALK KRVEPLAEEL GAFVAGHCDV ADAASIDAVF ETLEKKWGKL DFLVHAIGFS DKDELTGRYI DTSEANFTNT MLISVYSLTA VSRRAEKLMA DGGSILTLTY YGAEKVMPNY NVMGVAKAAL EASVKYLAVD LGPQNIRVNA ISAGPIKTLA ASGIGDFRYI LKWNEYNAPL RRTVTIDEVG DVGLYFLSDL SRSVTGEVHH ADSGYHVIGM KAVDAPDISV VKD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgcacag tctggtttgt tgcagggcaa gcgtggattg attctaggcg tggccaataa tcgctctatc gcttggggca ttgcaaaagc cgcccgtgaa gctggcgcag agcttgcatt cacctatcag ggtgacgcat tgaagaagcg tgtggagccg ctggccgagg agcttggcgc cttcgtcgcc ggacattgcg acgtcgccga tgcggcgagc atcgatgcgg tttttgaaac gcttgagaag aagtggggca agctcgactt cctggtgcac gcaatcggtt tctccgacaa ggacgagctg accggccgct atatcgatac gtcggaagcc aatttcacca atacgatgct gatttcggtc tattcgctga cggcggtttc gcgccgcgcg gaaaagctga tggccgatgg cggatcgatt ctgacgctga cctattacgg cgctgaaaag gtcatgccga attacaatgt catgggcgtg gccaaggcag cccttgaggc gagcgtgaaa tatctggccg tcgatcttgg cccccagaac attcgcgtca atgcaatctc ggctggcccc atcaagacgc ttgccgcttc cggcattggt gacttccgct atatcctgaa gtggaacgaa tataatgcgc cgctgcgccg caccgtcacg atcgacgagg tgggtgatgt cggcctttat ttcctttcgg acctgtcgcg ttcggttaca ggcgaagtgc accatgccga cagcggctat catgtcattg gcatgaaagc ggttgacgca ccggatatct cggtcgtgaa agactgaaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for BrabA.00010.a.B1.GE25459
HARVESTED ON: 7/11/2008
SEQUENCED ON: 3/6/2009
EXPECTED MW: 30kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMT AQSGLLQGKR GLILGVANNR SIAWGIAKAA REAGAELAFT YQGDALKKRV EPLAEELGAF VAGHCDVADA ASIDAVFETL EKKWGKLDFL VHAIGFSDKD ELTGRYIDTS EANFTNTMLI SVYSLTAVSR RAEKLMADGG SILTLTYYGA EKVMPNYNVM GVAKAALEAS VKYLAVDLGP QNIRVNAISA GPIKTLAASG IGDFRYILKW NEYNAPLRRT VTIDEVGDVG LYFLSDLSRS VTGEVHHADS GYHVIGMKAV DAPDISVVKD
Validated NT Sequence
atggctcacc accaccacca ccatatgacc gcacagtctg gtttgttgca gggcaagcgt ggattgattc taggcgtggc caataatcgc tctatcgctt ggggcattgc aaaagccgcc cgtgaagctg gcgcagagct tgcattcacc tatcagggtg acgcattgaa gaagcgtgtg gagccgctgg ccgaggagct tggcgccttc gtcgccggac attgcgacgt cgccgatgcg gcgagcatcg atgcggtttt tgaaacgctt gagaagaagt ggggcaagct cgacttcctg gtgcacgcaa tcggtttctc cgacaaggac gagctgaccg gccgctatat cgatacgtcg gaagccaatt tcaccaatac gatgctgatt tcggtctatt cgctgacggc ggtttcgcgc cgcgcggaaa agctgatggc cgatggcgga tcgattctga cgctgaccta ttacggcgct gaaaaggtca tgccgaatta caatgtcatg ggcgtggcca aggcagccct tgaggcgagc gtgaaatatc tggccgtcga tcttggcccc cagaacattc gcgtcaatgc aatctcggct ggccccatca agacgcttgc cgcttccggc attggtgact tccgctatat cctgaagtgg aacgaatata atgcgccgct gcgccgcacc gtcacgatcg acgaggtggg tgatgtcggc ctttatttcc tttcggacct gtcgcgttcg gttacaggcg aagtgcacca tgccgacagc ggctatcatg tcattggcat gaaagcggtt gacgcaccgg atatctcggt cgtgaaagac taagtgagta agat
Expected Protein Sequence
MAHHHHHHMT AQSGLLQGKR GLILGVANNR SIAWGIAKAA REAGAELAFT YQGDALKKRV EPLAEELGAF VAGHCDVADA ASIDAVFETL EKKWGKLDFL VHAIGFSDKD ELTGRYIDTS EANFTNTMLI SVYSLTAVSR RAEKLMADGG SILTLTYYGA EKVMPNYNVM GVAKAALEAS VKYLAVDLGP QNIRVNAISA GPIKTLAASG IGDFRYILKW NEYNAPLRRT VTIDEVGDVG LYFLSDLSRS VTGEVHHADS GYHVIGMKAV DAPDISVVKD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccg cacagtctgg tttgttgcag ggcaagcgtg gattgattct aggcgtggcc aataatcgct ctatcgcttg gggcattgca aaagccgccc gtgaagctgg cgcagagctt gcattcacct atcagggtga cgcattgaag aagcgtgtgg agccgctggc cgaggagctt ggcgccttcg tcgccggaca ttgcgacgtc gccgatgcgg cgagcatcga tgcggttttt gaaacgcttg agaagaagtg gggcaagctc gacttcctgg tgcacgcaat cggtttctcc gacaaggacg agctgaccgg ccgctatatc gatacgtcgg aagccaattt caccaatacg atgctgattt cggtctattc gctgacggcg gtttcgcgcc gcgcggaaaa gctgatggcc gatggcggat cgattctgac gctgacctat tacggcgctg aaaaggtcat gccgaattac aatgtcatgg gcgtggccaa ggcagccctt gaggcgagcg tgaaatatct ggccgtcgat cttggccccc agaacattcg cgtcaatgca atctcggctg gccccatcaa gacgcttgcc gcttccggca ttggtgactt ccgctatatc ctgaagtgga acgaatataa tgcgccgctg cgccgcaccg tcacgatcga cgaggtgggt gatgtcggcc tttatttcct ttcggacctg tcgcgttcgg ttacaggcga agtgcaccat gccgacagcg gctatcatgt cattggcatg aaagcggttg acgcaccgga tatctcggtc gtgaaagact gatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for BrabA.00010.a.A1.PS00048
PURIFICATION DATe: 7/31/2008
CONCENTRATION: 47.25mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 100µl
PERCENT IDENTITY: 95
PERCENT COVERAGE: 96
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMTAQSGL LQGKRGLILG VANNRSIAWG IAKAAREAGA ELAFTYQGDA LKKRVEPLAE ELGAFVAGHC DVADAASIDA VFETLEKKWG KLDFLVHAIG FSDKDELTGR YIDTSEANFT NTMLISVYSL TAVSRRAEKL MADGGSILTL TYYGAEKVMP NYNVMGVAKA ALEASVKYLA VDLGPQNIRV NAISAGPIKT LAASGIXDFR YILKXNEYNA PLRXXVTIDX VGDVGLYFLS XXSRSVXGEV HHADSXYHXI GXKXVXAPDX SVV
Validated NT Sequence
tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgaccgc acagtctggt ttgttgcagg gcaagcgtgg attgattcta ggcgtggcca ataatcgctc tatcgcttgg ggcattgcaa aagccgcccg tgaagctggc gcagagcttg cattcaccta tcagggtgac gcattgaaga agcgtgtgga gccgctggcc gaggagcttg gcgccttcgt cgccggacat tgcgacgtcg ccgatgcggc gagcatcgat gcggtttttg aaacgcttga gaagaagtgg ggcaagctcg acttcctggt gcacgcaatc ggtttctccg acaaggacga gctgaccggc cgctatatcg atacgtcgga agccaatttc accaatacga tgctgatttc ggtctattcg ctgacggcgg tttcgcgccg cgcggaaaag ctgatggccg atggcggatc gattctgacg ctgacctatt acggcgctga aaaggtcatg ccgaattaca atgtcatggg cgtggccaag gcagcccttg aggcgagcgt gaaatatctg gccgtcgatc ttggccccca gaacattcgc gtcaatgcaa tctcggctgg ccccatcaag acgcttgccg cttccggcat tgntgacttc cgctatatcc tgaagtgnaa cgaatataat gcgccgctgc gccncancgt cacgatcgac gangtgggtg atgtcggcct ttatttcctt tcgnanntgt cgcgttcggt tncnggcgaa gtgcaccatg ccgacagcng ctatcatgnc attggnnnga aancggttga ngcaccggat nnctcggtcg tga
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTAQSGLLQ GKRGLILGVA NNRSIAWGIA KAAREAGAEL AFTYQGDALK KRVEPLAEEL GAFVAGHCDV ADAASIDAVF ETLEKKWGKL DFLVHAIGFS DKDELTGRYI DTSEANFTNT MLISVYSLTA VSRRAEKLMA DGGSILTLTY YGAEKVMPNY NVMGVAKAAL EASVKYLAVD LGPQNIRVNA ISAGPIKTLA ASGIGDFRYI LKWNEYNAPL RRTVTIDEVG DVGLYFLSDL SRSVTGEVHH ADSGYHVIGM KAVDAPDISV VKD
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCGCACAG TCTGGTTTGT TGCAGGGCAA GCGTGGATTG ATTCTAGGCG TGGCCAATAA TCGCTCTATC GCTTGGGGCA TTGCAAAAGC CGCCCGTGAA GCTGGCGCAG AGCTTGCATT CACCTATCAG GGTGACGCAT TGAAGAAGCG TGTGGAGCCG CTGGCCGAGG AGCTTGGCGC CTTCGTCGCC GGACATTGCG ACGTCGCCGA TGCGGCGAGC ATCGATGCGG TTTTTGAAAC GCTTGAGAAG AAGTGGGGCA AGCTCGACTT CCTGGTGCAC GCAATCGGTT TCTCCGACAA GGACGAGCTG ACCGGCCGCT ATATCGATAC GTCGGAAGCC AATTTCACCA ATACGATGCT GATTTCGGTC TATTCGCTGA CGGCGGTTTC GCGCCGCGCG GAAAAGCTGA TGGCCGATGG CGGATCGATT CTGACGCTGA CCTATTACGG CGCTGAAAAG GTCATGCCGA ATTACAATGT CATGGGCGTG GCCAAGGCAG CCCTTGAGGC GAGCGTGAAA TATCTGGCCG TCGATCTTGG CCCCCAGAAC ATTCGCGTCA ATGCAATCTC GGCTGGCCCC ATCAAGACGC TTGCCGCTTC CGGCATTGGT GACTTCCGCT ATATCCTGAA GTGGAACGAA TATAATGCGC CGCTGCGCCG CACCGTCACG ATCGACGAGG TGGGTGATGT CGGCCTTTAT TTCCTTTCGG ACCTGTCGCG TTCGGTTACA GGCGAAGTGC ACCATGCCGA CAGCGGCTAT CATGTCATTG GCATGAAAGC GGTTGACGCA CCGGATATCT CGGTCGTGAA AGACTGAAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA