BrabA.00008.a

Glycine hydroxymethyltransferase

CENTER ID: BrabA.00008.a
ORGANISM: Brucella melitensis biovar Abortus 2308
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: diffraction
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.00008.a.A1.GE25211 Full length( BrabA.00008.a ) 1 438
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrabA.00008.a.A1.PS00360 Full length( BrabA.00008.a ) 1 438
BrabA.00008.a.A1.PW25249 Full length( BrabA.00008.a ) 1 438
BrabA.00008.a.A1.PW27081 Full length( BrabA.00008.a ) 1 438

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|359391.4.peg.820
RefSeq: YP_414220
UniProt: Q2YN95

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSQANAATKA YSDVFFNASL EDIDPEIFGA IRNELGRQRH EIELIASENI VSRAVLEAQG SILTNKYAEG YPGKRYYGGC QYVDVVEELA IERAKKLFGA EFANVQPNSG SQMNQAVFLA LLQPGDTFMG LDLNSGGHLT HGSPVNMSGK WFNVVSYGVR KDDHLLDMDE VARLARENKP KLILAGGTAY SRIWDWKRFR EIADEVGAYL MVDMAHIAGL VAGGQHPSPV PHAHVCTTTT HKSLRGPRGG MILTNDADIA KKINSAVFPG LQGGPLMHVI AGKAVAFAEA LKPEFKLYAK NVVDNARALA EELKSHGLDI VSGGTDNHLM LVDLRPKNAT GKRAEAALGR ANITCNKNGI PFDPEKPFVT SGVRLGTPAG TTRGFGVAEF KEIGSLIAEV LDGLKVANSD EGNAAVEQAV KEKVIALTGR FPMYGYQG
NT Sequence
ATGTCTCAAG CCAACGCCGC CACGAAAGCG TATTCGGACG TTTTCTTCAA TGCAAGCCTC GAGGACATCG ATCCCGAGAT TTTCGGTGCA ATCCGCAACG AACTTGGTCG CCAGCGCCAT GAAATCGAGC TGATCGCTTC GGAAAACATC GTTTCGCGCG CTGTTCTGGA AGCACAGGGT TCCATCCTCA CCAACAAATA TGCCGAAGGC TATCCGGGCA AGCGCTATTA TGGCGGCTGC CAGTATGTCG ATGTCGTGGA AGAACTGGCG ATCGAACGCG CCAAGAAGCT TTTCGGTGCC GAATTCGCCA ATGTTCAGCC GAATTCCGGC AGCCAGATGA ATCAGGCGGT TTTCCTCGCG CTGCTTCAGC CAGGCGATAC CTTCATGGGC CTCGACCTTA ATTCAGGTGG CCACCTGACC CACGGTTCGC CGGTCAATAT GTCCGGCAAG TGGTTCAATG TCGTGTCCTA CGGCGTGCGC AAGGACGATC ATCTGCTCGA CATGGACGAA GTCGCCCGCC TCGCACGCGA AAACAAGCCC AAGCTTATCC TTGCAGGCGG CACGGCCTAT TCGCGTATCT GGGACTGGAA GCGTTTCCGC GAGATCGCCG ATGAAGTGGG CGCTTATCTC ATGGTCGATA TGGCGCATAT TGCCGGTCTC GTGGCTGGCG GTCAGCATCC GTCGCCGGTT CCCCATGCCC ATGTCTGCAC CACGACAACG CACAAATCGC TTCGCGGTCC GCGCGGCGGC ATGATCCTCA CCAATGATGC CGATATTGCC AAGAAGATCA ATTCGGCTGT CTTCCCCGGC CTTCAGGGCG GCCCGCTGAT GCATGTCATC GCCGGTAAGG CTGTTGCCTT TGCCGAGGCG TTGAAGCCTG AGTTCAAGCT CTATGCGAAG AATGTGGTGG ATAACGCCCG TGCGCTTGCC GAGGAATTGA AGTCGCACGG CCTCGACATT GTTTCGGGCG GCACCGACAA CCATCTCATG CTGGTCGATC TGCGCCCGAA GAACGCAACC GGCAAGCGGG CGGAAGCCGC TCTCGGACGC GCCAACATCA CCTGCAACAA GAACGGCATT CCCTTCGACC CTGAAAAGCC GTTTGTGACC TCCGGCGTCC GTCTTGGCAC GCCTGCCGGC ACCACGCGCG GCTTCGGCGT TGCGGAATTC AAGGAAATCG GTTCGCTGAT CGCCGAGGTT CTCGACGGTT TGAAGGTCGC CAATTCCGAC GAAGGCAATG CGGCTGTCGA ACAGGCCGTG AAGGAAAAGG TCATCGCTCT GACCGGCCGT TTCCCGATGT ACGGCTATCA GGGA
Details for BrabA.00008.a.A1.GE25211
HARVESTED ON: 5/6/2008
SEQUENCED ON: 7/29/2008
EXPECTED MW: 49kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSQANAATK AYSDVFFNAS LEDIDPEIFG AIRNELGRQR HEIELIASEN IVSRAVLEAQ GSILTNKYAE GYPGKRYYGG CQYVDVVEEL AIERAKKLFG AEFANVQPNS GSQMNQAVFL ALLQPGDTFM GLDLNSGGHL THGSPVNMSG KWFNVVSYGV RKDDHLLDMD EVARLARENK PKLILAGGTA YSRIWDWKRF REIADEVGAY LMVDMAHIAG LVAGGQHPSP VPHAHVCTTT THKSLRGPRG GMILTNDADI AKKINSAVFP GLQGGPLMHV IAGKAVAFAE ALKPEFKLYA KNVVDNARAL AEELKSHGLD IVSGGTDNHL MLVDLRPKNA TGKRAEAALG RANITCNKNG IPFDPEKPFV TSGVRLGTPA GTTRGFGVAE FKEIGSLIAE VLDGLKVANS DEGNAAVEQA VKEKVIALTG RFPMYG
Validated NT Sequence
tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgtctca agccaacgcc gccacgaaag cgtattcgga cgttttcttc aatgcaagcc tcgaggacat cgatcccgag attttcggtg caatccgcaa cgaacttggt cgccagcgcc atgaaatcga gctgatcgct tcggaaaaca tcgtttcgcg cgctgttctg gaagcacagg gttccatcct caccaacaaa tatgccgaag gctatccggg caagcgctat tatggcggct gccagtatgt cgatgtcgtg gaagaactgg cgatcgaacg cgccaagaag ctgttcggtg ccgaattcgc caatgttcag ccgaattccg gcagccagat gaatcaggcg gttttcctcg cgctgcttca gccaggcgat accttcatgg gcctcgacct taattcaggt ggccacctga cccacggttc gccggtcaat atgtccggca agtggttcaa tgtcgtgtcc tacggcgtgc gcaaggacga tcatctgctc gacatggacg aagtcgcccg cctcgcacgc gaaaacaagc ccaagctgat ccttgcaggc ggcacggcct attcgcgtat ctgggactgg aagcgtttcc gcgagatcgc cgatgaagtg ggcgcctatc tcatggtcga tatggcgcat attgccggtc tcgtggctgg cggtcagcat ccgtcgccgg ttccccatgc ccatgtctgc accacgacaa cgcacaaatc gcttcgcggt ccgcgcggcg gcatgatcct caccaatgat gccgatattg ccaagaagat caattcggct gtcttccccg gccttcaggg cggcccgctg atgcatgtca tcgccggtaa ggctgttgcc tttgccgagg cgttgaagcc tgagttcaag ctctatgcga agaatgtggt ggataacgcc cgtgcgcttg ccgaggaatt gaagtcgcac ggcctcgaca ttgtttcggg cggcaccgac aaccatctca tgctggtcga tctgcgcccg aagaacgcaa ccggcaagcg ggcggaagcc gctctcggac gcgccaacat cacctgcaac aagaacggca ttcccttcga ccctgaaaag ccgtttgtga cctccggcgt ccgtcttggc acgcctgccg gcaccacgcg cggcttcggc gttgcggaat tcaaggaaat cggttcgctg atcgccgagg ttctcgacgg tttgaaggtc gccaattccg acgaaggcaa tgcggctgtc gaacaggccg tgaaggaaaa ggtcatcgct ctgaccggcc gtttcccgat gtacggctat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSQANAATK AYSDVFFNAS LEDIDPEIFG AIRNELGRQR HEIELIASEN IVSRAVLEAQ GSILTNKYAE GYPGKRYYGG CQYVDVVEEL AIERAKKLFG AEFANVQPNS GSQMNQAVFL ALLQPGDTFM GLDLNSGGHL THGSPVNMSG KWFNVVSYGV RKDDHLLDMD EVARLARENK PKLILAGGTA YSRIWDWKRF REIADEVGAY LMVDMAHIAG LVAGGQHPSP VPHAHVCTTT THKSLRGPRG GMILTNDADI AKKINSAVFP GLQGGPLMHV IAGKAVAFAE ALKPEFKLYA KNVVDNARAL AEELKSHGLD IVSGGTDNHL MLVDLRPKNA TGKRAEAALG RANITCNKNG IPFDPEKPFV TSGVRLGTPA GTTRGFGVAE FKEIGSLIAE VLDGLKVANS DEGNAAVEQA VKEKVIALTG RFPMYGYQG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctcaagcc aacgccgcca cgaaagcgta ttcggacgtt ttcttcaatg caagcctcga ggacatcgat cccgagattt tcggtgcaat ccgcaacgaa cttggtcgcc agcgccatga aatcgagctg atcgcttcgg aaaacatcgt ttcgcgcgct gttctggaag cacagggttc catcctcacc aacaaatatg ccgaaggcta tccgggcaag cgctattatg gcggctgcca gtatgtcgat gtcgtggaag aactggcgat cgaacgcgcc aagaagctgt tcggtgccga attcgccaat gttcagccga attccggcag ccagatgaat caggcggttt tcctcgcgct gcttcagcca ggcgatacct tcatgggcct cgaccttaat tcaggtggcc acctgaccca cggttcgccg gtcaatatgt ccggcaagtg gttcaatgtc gtgtcctacg gcgtgcgcaa ggacgatcat ctgctcgaca tggacgaagt cgcccgcctc gcacgcgaaa acaagcccaa gctgatcctt gcaggcggca cggcctattc gcgtatctgg gactggaagc gtttccgcga gatcgccgat gaagtgggcg cctatctcat ggtcgatatg gcgcatattg ccggtctcgt ggctggcggt cagcatccgt cgccggttcc ccatgcccat gtctgcacca cgacaacgca caaatcgctt cgcggtccgc gcggcggcat gatcctcacc aatgatgccg atattgccaa gaagatcaat tcggctgtct tccccggcct tcagggcggc ccgctgatgc atgtcatcgc cggtaaggct gttgcctttg ccgaggcgtt gaagcctgag ttcaagctct atgcgaagaa tgtggtggat aacgcccgtg cgcttgccga ggaattgaag tcgcacggcc tcgacattgt ttcgggcggc accgacaacc atctcatgct ggtcgatctg cgcccgaaga acgcaaccgg caagcgggcg gaagccgctc tcggacgcgc caacatcacc tgcaacaaga acggcattcc cttcgaccct gaaaagccgt ttgtgacctc cggcgtccgt cttggcacgc ctgccggcac cacgcgcggc ttcggcgttg cggaattcaa ggaaatcggt tcgctgatcg ccgaggttct cgacggtttg aaggtcgcca attccgacga aggcaatgcg gctgtcgaac aggccgtgaa ggaaaaggtc atcgctctga ccggccgttt cccgatgtac ggctatcagg gataaaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BrabA.00008.a.A1.PS00360
PURIFICATION DATe: 9/21/2009
CONCENTRATION: 37.18mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 10
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSQANAATK AYSDVFFNAS LEDIDPEIFG AIRNELGRQR HEIELIASEN IVSRAVLEAQ GSILTNKYAE GYPGKRYYGG CQYVDVVEEL AIERAKKLFG AEFANVQPNS GSQMNQAVFL ALLQPGDTFM GLDLNSGGHL THGSPVNMSG KWFNVVSYGV RKDDHLLDMD EVARLARENK PKLILAGGTA YSRIWDWKRF REIADEVGAY LMVDMAHIAG LVAGGQHPSP VPHAHVCTTT THKSLRGPRG GMILTNDADI AKKINSAVFP GLQGGPLMHV IAGKAVAFAE ALKPEFKLYA KNVVDNARAL AEELKSHGLD IVSGGTDNHL MLVDLRPKNA TGKRAEAALG RANITCNKNG IPFDPEKPFV TSGVRLGTPA GTTRGFGVAE FKEIGSLIAE VLDGLKVANS DEGNAAVEQA VKEKVIALTG RFPMYG
Validated NT Sequence
tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgtctca agccaacgcc gccacgaaag cgtattcgga cgttttcttc aatgcaagcc tcgaggacat cgatcccgag attttcggtg caatccgcaa cgaacttggt cgccagcgcc atgaaatcga gctgatcgct tcggaaaaca tcgtttcgcg cgctgttctg gaagcacagg gttccatcct caccaacaaa tatgccgaag gctatccggg caagcgctat tatggcggct gccagtatgt cgatgtcgtg gaagaactgg cgatcgaacg cgccaagaag ctgttcggtg ccgaattcgc caatgttcag ccgaattccg gcagccagat gaatcaggcg gttttcctcg cgctgcttca gccaggcgat accttcatgg gcctcgacct taattcaggt ggccacctga cccacggttc gccggtcaat atgtccggca agtggttcaa tgtcgtgtcc tacggcgtgc gcaaggacga tcatctgctc gacatggacg aagtcgcccg cctcgcacgc gaaaacaagc ccaagctgat ccttgcaggc ggcacggcct attcgcgtat ctgggactgg aagcgtttcc gcgagatcgc cgatgaagtg ggcgcctatc tcatggtcga tatggcgcat attgccggtc tcgtggctgg cggtcagcat ccgtcgccgg ttccccatgc ccatgtctgc accacgacaa cgcacaaatc gcttcgcggt ccgcgcggcg gcatgatcct caccaatgat gccgatattg ccaagaagat caattcggct gtcttccccg gccttcaggg cggcccgctg atgcatgtca tcgccggtaa ggctgttgcc tttgccgagg cgttgaagcc tgagttcaag ctctatgcga agaatgtggt ggataacgcc cgtgcgcttg ccgaggaatt gaagtcgcac ggcctcgaca ttgtttcggg cggcaccgac aaccatctca tgctggtcga tctgcgcccg aagaacgcaa ccggcaagcg ggcggaagcc gctctcggac gcgccaacat cacctgcaac aagaacggca ttcccttcga ccctgaaaag ccgtttgtga cctccggcgt ccgtcttggc acgcctgccg gcaccacgcg cggcttcggc gttgcggaat tcaaggaaat cggttcgctg atcgccgagg ttctcgacgg tttgaaggtc gccaattccg acgaaggcaa tgcggctgtc gaacaggccg tgaaggaaaa ggtcatcgct ctgaccggcc gtttcccgat gtacggctat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSQANAATK AYSDVFFNAS LEDIDPEIFG AIRNELGRQR HEIELIASEN IVSRAVLEAQ GSILTNKYAE GYPGKRYYGG CQYVDVVEEL AIERAKKLFG AEFANVQPNS GSQMNQAVFL ALLQPGDTFM GLDLNSGGHL THGSPVNMSG KWFNVVSYGV RKDDHLLDMD EVARLARENK PKLILAGGTA YSRIWDWKRF REIADEVGAY LMVDMAHIAG LVAGGQHPSP VPHAHVCTTT THKSLRGPRG GMILTNDADI AKKINSAVFP GLQGGPLMHV IAGKAVAFAE ALKPEFKLYA KNVVDNARAL AEELKSHGLD IVSGGTDNHL MLVDLRPKNA TGKRAEAALG RANITCNKNG IPFDPEKPFV TSGVRLGTPA GTTRGFGVAE FKEIGSLIAE VLDGLKVANS DEGNAAVEQA VKEKVIALTG RFPMYGYQG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCTCAAGCC AACGCCGCCA CGAAAGCGTA TTCGGACGTT TTCTTCAATG CAAGCCTCGA GGACATCGAT CCCGAGATTT TCGGTGCAAT CCGCAACGAA CTTGGTCGCC AGCGCCATGA AATCGAGCTG ATCGCTTCGG AAAACATCGT TTCGCGCGCT GTTCTGGAAG CACAGGGTTC CATCCTCACC AACAAATATG CCGAAGGCTA TCCGGGCAAG CGCTATTATG GCGGCTGCCA GTATGTCGAT GTCGTGGAAG AACTGGCGAT CGAACGCGCC AAGAAGCTGT TCGGTGCCGA ATTCGCCAAT GTTCAGCCGA ATTCCGGCAG CCAGATGAAT CAGGCGGTTT TCCTCGCGCT GCTTCAGCCA GGCGATACCT TCATGGGCCT CGACCTTAAT TCAGGTGGCC ACCTGACCCA CGGTTCGCCG GTCAATATGT CCGGCAAGTG GTTCAATGTC GTGTCCTACG GCGTGCGCAA GGACGATCAT CTGCTCGACA TGGACGAAGT CGCCCGCCTC GCACGCGAAA ACAAGCCCAA GCTGATCCTT GCAGGCGGCA CGGCCTATTC GCGTATCTGG GACTGGAAGC GTTTCCGCGA GATCGCCGAT GAAGTGGGCG CCTATCTCAT GGTCGATATG GCGCATATTG CCGGTCTCGT GGCTGGCGGT CAGCATCCGT CGCCGGTTCC CCATGCCCAT GTCTGCACCA CGACAACGCA CAAATCGCTT CGCGGTCCGC GCGGCGGCAT GATCCTCACC AATGATGCCG ATATTGCCAA GAAGATCAAT TCGGCTGTCT TCCCCGGCCT TCAGGGCGGC CCGCTGATGC ATGTCATCGC CGGTAAGGCT GTTGCCTTTG CCGAGGCGTT GAAGCCTGAG TTCAAGCTCT ATGCGAAGAA TGTGGTGGAT AACGCCCGTG CGCTTGCCGA GGAATTGAAG TCGCACGGCC TCGACATTGT TTCGGGCGGC ACCGACAACC ATCTCATGCT GGTCGATCTG CGCCCGAAGA ACGCAACCGG CAAGCGGGCG GAAGCCGCTC TCGGACGCGC CAACATCACC TGCAACAAGA ACGGCATTCC CTTCGACCCT GAAAAGCCGT TTGTGACCTC CGGCGTCCGT CTTGGCACGC CTGCCGGCAC CACGCGCGGC TTCGGCGTTG CGGAATTCAA GGAAATCGGT TCGCTGATCG CCGAGGTTCT CGACGGTTTG AAGGTCGCCA ATTCCGACGA AGGCAATGCG GCTGTCGAAC AGGCCGTGAA GGAAAAGGTC ATCGCTCTGA CCGGCCGTTT CCCGATGTAC GGCTATCAGG GATAAAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA
Details for BrabA.00008.a.A1.PW25249
PURIFICATION DATe: 6/16/2008
CONCENTRATION: 25.4mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 40
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSQANAATK AYSDVFFNAS LEDIDPEIFG AIRNELGRQR HEIELIASEN IVSRAVLEAQ GSILTNKYAE GYPGKRYYGG CQYVDVVEEL AIERAKKLFG AEFANVQPNS GSQMNQAVFL ALLQPGDTFM GLDLNSGGHL THGSPVNMSG KWFNVVSYGV RKDDHLLDMD EVARLARENK PKLILAGGTA YSRIWDWKRF REIADEVGAY LMVDMAHIAG LVAGGQHPSP VPHAHVCTTT THKSLRGPRG GMILTNDADI AKKINSAVFP GLQGGPLMHV IAGKAVAFAE ALKPEFKLYA KNVVDNARAL AEELKSHGLD IVSGGTDNHL MLVDLRPKNA TGKRAEAALG RANITCNKNG IPFDPEKPFV TSGVRLGTPA GTTRGFGVAE FKEIGSLIAE VLDGLKVANS DEGNAAVEQA VKEKVIALTG RFPMYG
Validated NT Sequence
tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgtctca agccaacgcc gccacgaaag cgtattcgga cgttttcttc aatgcaagcc tcgaggacat cgatcccgag attttcggtg caatccgcaa cgaacttggt cgccagcgcc atgaaatcga gctgatcgct tcggaaaaca tcgtttcgcg cgctgttctg gaagcacagg gttccatcct caccaacaaa tatgccgaag gctatccggg caagcgctat tatggcggct gccagtatgt cgatgtcgtg gaagaactgg cgatcgaacg cgccaagaag ctgttcggtg ccgaattcgc caatgttcag ccgaattccg gcagccagat gaatcaggcg gttttcctcg cgctgcttca gccaggcgat accttcatgg gcctcgacct taattcaggt ggccacctga cccacggttc gccggtcaat atgtccggca agtggttcaa tgtcgtgtcc tacggcgtgc gcaaggacga tcatctgctc gacatggacg aagtcgcccg cctcgcacgc gaaaacaagc ccaagctgat ccttgcaggc ggcacggcct attcgcgtat ctgggactgg aagcgtttcc gcgagatcgc cgatgaagtg ggcgcctatc tcatggtcga tatggcgcat attgccggtc tcgtggctgg cggtcagcat ccgtcgccgg ttccccatgc ccatgtctgc accacgacaa cgcacaaatc gcttcgcggt ccgcgcggcg gcatgatcct caccaatgat gccgatattg ccaagaagat caattcggct gtcttccccg gccttcaggg cggcccgctg atgcatgtca tcgccggtaa ggctgttgcc tttgccgagg cgttgaagcc tgagttcaag ctctatgcga agaatgtggt ggataacgcc cgtgcgcttg ccgaggaatt gaagtcgcac ggcctcgaca ttgtttcggg cggcaccgac aaccatctca tgctggtcga tctgcgcccg aagaacgcaa ccggcaagcg ggcggaagcc gctctcggac gcgccaacat cacctgcaac aagaacggca ttcccttcga ccctgaaaag ccgtttgtga cctccggcgt ccgtcttggc acgcctgccg gcaccacgcg cggcttcggc gttgcggaat tcaaggaaat cggttcgctg atcgccgagg ttctcgacgg tttgaaggtc gccaattccg acgaaggcaa tgcggctgtc gaacaggccg tgaaggaaaa ggtcatcgct ctgaccggcc gtttcccgat gtacggctat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSQANAATK AYSDVFFNAS LEDIDPEIFG AIRNELGRQR HEIELIASEN IVSRAVLEAQ GSILTNKYAE GYPGKRYYGG CQYVDVVEEL AIERAKKLFG AEFANVQPNS GSQMNQAVFL ALLQPGDTFM GLDLNSGGHL THGSPVNMSG KWFNVVSYGV RKDDHLLDMD EVARLARENK PKLILAGGTA YSRIWDWKRF REIADEVGAY LMVDMAHIAG LVAGGQHPSP VPHAHVCTTT THKSLRGPRG GMILTNDADI AKKINSAVFP GLQGGPLMHV IAGKAVAFAE ALKPEFKLYA KNVVDNARAL AEELKSHGLD IVSGGTDNHL MLVDLRPKNA TGKRAEAALG RANITCNKNG IPFDPEKPFV TSGVRLGTPA GTTRGFGVAE FKEIGSLIAE VLDGLKVANS DEGNAAVEQA VKEKVIALTG RFPMYGYQG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCTCAAGCC AACGCCGCCA CGAAAGCGTA TTCGGACGTT TTCTTCAATG CAAGCCTCGA GGACATCGAT CCCGAGATTT TCGGTGCAAT CCGCAACGAA CTTGGTCGCC AGCGCCATGA AATCGAGCTG ATCGCTTCGG AAAACATCGT TTCGCGCGCT GTTCTGGAAG CACAGGGTTC CATCCTCACC AACAAATATG CCGAAGGCTA TCCGGGCAAG CGCTATTATG GCGGCTGCCA GTATGTCGAT GTCGTGGAAG AACTGGCGAT CGAACGCGCC AAGAAGCTGT TCGGTGCCGA ATTCGCCAAT GTTCAGCCGA ATTCCGGCAG CCAGATGAAT CAGGCGGTTT TCCTCGCGCT GCTTCAGCCA GGCGATACCT TCATGGGCCT CGACCTTAAT TCAGGTGGCC ACCTGACCCA CGGTTCGCCG GTCAATATGT CCGGCAAGTG GTTCAATGTC GTGTCCTACG GCGTGCGCAA GGACGATCAT CTGCTCGACA TGGACGAAGT CGCCCGCCTC GCACGCGAAA ACAAGCCCAA GCTGATCCTT GCAGGCGGCA CGGCCTATTC GCGTATCTGG GACTGGAAGC GTTTCCGCGA GATCGCCGAT GAAGTGGGCG CCTATCTCAT GGTCGATATG GCGCATATTG CCGGTCTCGT GGCTGGCGGT CAGCATCCGT CGCCGGTTCC CCATGCCCAT GTCTGCACCA CGACAACGCA CAAATCGCTT CGCGGTCCGC GCGGCGGCAT GATCCTCACC AATGATGCCG ATATTGCCAA GAAGATCAAT TCGGCTGTCT TCCCCGGCCT TCAGGGCGGC CCGCTGATGC ATGTCATCGC CGGTAAGGCT GTTGCCTTTG CCGAGGCGTT GAAGCCTGAG TTCAAGCTCT ATGCGAAGAA TGTGGTGGAT AACGCCCGTG CGCTTGCCGA GGAATTGAAG TCGCACGGCC TCGACATTGT TTCGGGCGGC ACCGACAACC ATCTCATGCT GGTCGATCTG CGCCCGAAGA ACGCAACCGG CAAGCGGGCG GAAGCCGCTC TCGGACGCGC CAACATCACC TGCAACAAGA ACGGCATTCC CTTCGACCCT GAAAAGCCGT TTGTGACCTC CGGCGTCCGT CTTGGCACGC CTGCCGGCAC CACGCGCGGC TTCGGCGTTG CGGAATTCAA GGAAATCGGT TCGCTGATCG CCGAGGTTCT CGACGGTTTG AAGGTCGCCA ATTCCGACGA AGGCAATGCG GCTGTCGAAC AGGCCGTGAA GGAAAAGGTC ATCGCTCTGA CCGGCCGTTT CCCGATGTAC GGCTATCAGG GATAAAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA
Details for BrabA.00008.a.A1.PW27081
PURIFICATION DATe: 6/8/2009
CONCENTRATION: 3.1mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSQANAATK AYSDVFFNAS LEDIDPEIFG AIRNELGRQR HEIELIASEN IVSRAVLEAQ GSILTNKYAE GYPGKRYYGG CQYVDVVEEL AIERAKKLFG AEFANVQPNS GSQMNQAVFL ALLQPGDTFM GLDLNSGGHL THGSPVNMSG KWFNVVSYGV RKDDHLLDMD EVARLARENK PKLILAGGTA YSRIWDWKRF REIADEVGAY LMVDMAHIAG LVAGGQHPSP VPHAHVCTTT THKSLRGPRG GMILTNDADI AKKINSAVFP GLQGGPLMHV IAGKAVAFAE ALKPEFKLYA KNVVDNARAL AEELKSHGLD IVSGGTDNHL MLVDLRPKNA TGKRAEAALG RANITCNKNG IPFDPEKPFV TSGVRLGTPA GTTRGFGVAE FKEIGSLIAE VLDGLKVANS DEGNAAVEQA VKEKVIALTG RFPMYG
Validated NT Sequence
tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgtctca agccaacgcc gccacgaaag cgtattcgga cgttttcttc aatgcaagcc tcgaggacat cgatcccgag attttcggtg caatccgcaa cgaacttggt cgccagcgcc atgaaatcga gctgatcgct tcggaaaaca tcgtttcgcg cgctgttctg gaagcacagg gttccatcct caccaacaaa tatgccgaag gctatccggg caagcgctat tatggcggct gccagtatgt cgatgtcgtg gaagaactgg cgatcgaacg cgccaagaag ctgttcggtg ccgaattcgc caatgttcag ccgaattccg gcagccagat gaatcaggcg gttttcctcg cgctgcttca gccaggcgat accttcatgg gcctcgacct taattcaggt ggccacctga cccacggttc gccggtcaat atgtccggca agtggttcaa tgtcgtgtcc tacggcgtgc gcaaggacga tcatctgctc gacatggacg aagtcgcccg cctcgcacgc gaaaacaagc ccaagctgat ccttgcaggc ggcacggcct attcgcgtat ctgggactgg aagcgtttcc gcgagatcgc cgatgaagtg ggcgcctatc tcatggtcga tatggcgcat attgccggtc tcgtggctgg cggtcagcat ccgtcgccgg ttccccatgc ccatgtctgc accacgacaa cgcacaaatc gcttcgcggt ccgcgcggcg gcatgatcct caccaatgat gccgatattg ccaagaagat caattcggct gtcttccccg gccttcaggg cggcccgctg atgcatgtca tcgccggtaa ggctgttgcc tttgccgagg cgttgaagcc tgagttcaag ctctatgcga agaatgtggt ggataacgcc cgtgcgcttg ccgaggaatt gaagtcgcac ggcctcgaca ttgtttcggg cggcaccgac aaccatctca tgctggtcga tctgcgcccg aagaacgcaa ccggcaagcg ggcggaagcc gctctcggac gcgccaacat cacctgcaac aagaacggca ttcccttcga ccctgaaaag ccgtttgtga cctccggcgt ccgtcttggc acgcctgccg gcaccacgcg cggcttcggc gttgcggaat tcaaggaaat cggttcgctg atcgccgagg ttctcgacgg tttgaaggtc gccaattccg acgaaggcaa tgcggctgtc gaacaggccg tgaaggaaaa ggtcatcgct ctgaccggcc gtttcccgat gtacggctat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSQANAATK AYSDVFFNAS LEDIDPEIFG AIRNELGRQR HEIELIASEN IVSRAVLEAQ GSILTNKYAE GYPGKRYYGG CQYVDVVEEL AIERAKKLFG AEFANVQPNS GSQMNQAVFL ALLQPGDTFM GLDLNSGGHL THGSPVNMSG KWFNVVSYGV RKDDHLLDMD EVARLARENK PKLILAGGTA YSRIWDWKRF REIADEVGAY LMVDMAHIAG LVAGGQHPSP VPHAHVCTTT THKSLRGPRG GMILTNDADI AKKINSAVFP GLQGGPLMHV IAGKAVAFAE ALKPEFKLYA KNVVDNARAL AEELKSHGLD IVSGGTDNHL MLVDLRPKNA TGKRAEAALG RANITCNKNG IPFDPEKPFV TSGVRLGTPA GTTRGFGVAE FKEIGSLIAE VLDGLKVANS DEGNAAVEQA VKEKVIALTG RFPMYGYQG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCTCAAGCC AACGCCGCCA CGAAAGCGTA TTCGGACGTT TTCTTCAATG CAAGCCTCGA GGACATCGAT CCCGAGATTT TCGGTGCAAT CCGCAACGAA CTTGGTCGCC AGCGCCATGA AATCGAGCTG ATCGCTTCGG AAAACATCGT TTCGCGCGCT GTTCTGGAAG CACAGGGTTC CATCCTCACC AACAAATATG CCGAAGGCTA TCCGGGCAAG CGCTATTATG GCGGCTGCCA GTATGTCGAT GTCGTGGAAG AACTGGCGAT CGAACGCGCC AAGAAGCTGT TCGGTGCCGA ATTCGCCAAT GTTCAGCCGA ATTCCGGCAG CCAGATGAAT CAGGCGGTTT TCCTCGCGCT GCTTCAGCCA GGCGATACCT TCATGGGCCT CGACCTTAAT TCAGGTGGCC ACCTGACCCA CGGTTCGCCG GTCAATATGT CCGGCAAGTG GTTCAATGTC GTGTCCTACG GCGTGCGCAA GGACGATCAT CTGCTCGACA TGGACGAAGT CGCCCGCCTC GCACGCGAAA ACAAGCCCAA GCTGATCCTT GCAGGCGGCA CGGCCTATTC GCGTATCTGG GACTGGAAGC GTTTCCGCGA GATCGCCGAT GAAGTGGGCG CCTATCTCAT GGTCGATATG GCGCATATTG CCGGTCTCGT GGCTGGCGGT CAGCATCCGT CGCCGGTTCC CCATGCCCAT GTCTGCACCA CGACAACGCA CAAATCGCTT CGCGGTCCGC GCGGCGGCAT GATCCTCACC AATGATGCCG ATATTGCCAA GAAGATCAAT TCGGCTGTCT TCCCCGGCCT TCAGGGCGGC CCGCTGATGC ATGTCATCGC CGGTAAGGCT GTTGCCTTTG CCGAGGCGTT GAAGCCTGAG TTCAAGCTCT ATGCGAAGAA TGTGGTGGAT AACGCCCGTG CGCTTGCCGA GGAATTGAAG TCGCACGGCC TCGACATTGT TTCGGGCGGC ACCGACAACC ATCTCATGCT GGTCGATCTG CGCCCGAAGA ACGCAACCGG CAAGCGGGCG GAAGCCGCTC TCGGACGCGC CAACATCACC TGCAACAAGA ACGGCATTCC CTTCGACCCT GAAAAGCCGT TTGTGACCTC CGGCGTCCGT CTTGGCACGC CTGCCGGCAC CACGCGCGGC TTCGGCGTTG CGGAATTCAA GGAAATCGGT TCGCTGATCG CCGAGGTTCT CGACGGTTTG AAGGTCGCCA ATTCCGACGA AGGCAATGCG GCTGTCGAAC AGGCCGTGAA GGAAAAGGTC ATCGCTCTGA CCGGCCGTTT CCCGATGTAC GGCTATCAGG GATAAAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA