BrabA.00006.a
Precorrin-8X methylmutase CbiC/CobH
| CENTER ID: | BrabA.00006.a |
| ORGANISM: | Brucella melitensis biovar Abortus 2308 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.00006.a.A1.GE25210 | Full length( BrabA.00006.a ) | 1 | 208 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.00006.a.A1.PS00031 | Full length( BrabA.00006.a ) | 1 | 208 |
Structures
3E7D
DEPOSITED: 8/18/2008
DETERMINATION: XRay
CLONE: BrabA.00006.a.A1.GE25210
PROTEIN: BrabA.00006.a.A1.PS00031
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_136504 |
| PATRIC ID: | fig|359391.4.peg.1358 |
| RefSeq: | YP_414688 |
| UniProt: | Q2YRF5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDYIRDGQA IYDRSFAIIR AEADLRHIPA DLEKLAVRVI HACGMVDVAN DLAFSEGAGK AGRNALLAGA PILCDARMVA EGITRSRLPA DNRVIYTLSD PSVPELAKKI GNTRSAAALD LWLPHIEGSI VAIGNAPTAL FRLFELLDAG APKPALIIGM PVGFVGAAES KDELAANSRG VPYVIVRGRR GGSAMTAAAV NALASERE
NT Sequence
ATGACAGACT ATATCCGTGA CGGACAGGCC ATTTACGACC GATCCTTCGC TATTATCCGC GCCGAAGCCG ATCTCCGTCA CATTCCAGCC GATCTTGAAA AGCTTGCCGT TCGCGTGATT CACGCCTGCG GCATGGTGGA TGTGGCAAAC GATCTTGCCT TCTCGGAAGG TGCAGGCAAA GCCGGGCGCA ATGCTCTTCT GGCAGGCGCG CCGATCCTCT GCGATGCGCG CATGGTGGCC GAAGGCATCA CCCGCTCGCG CCTTCCCGCC GATAATCGCG TGATCTACAC CCTGAGCGAC CCATCCGTGC CGGAACTTGC AAAAAAGATC GGCAACACCC GCTCCGCAGC CGCGCTTGAT CTCTGGCTCC CGCATATTGA AGGCAGCATT GTCGCCATCG GCAATGCGCC GACCGCCCTC TTCCGCCTGT TCGAGCTTCT CGATGCAGGG GCACCGAAGC CCGCACTTAT CATTGGAATG CCGGTGGGCT TTGTGGGAGC GGCGGAATCG AAGGACGAGC TTGCCGCCAA CAGCCGCGGC GTGCCTTACG TGATCGTGCG CGGGCGGCGC GGCGGCAGCG CCATGACGGC AGCCGCGGTC AACGCGCTTG CTTCGGAGCG TGAA
Details for BrabA.00006.a.A1.GE25210
| HARVESTED ON: | 5/6/2008 |
| SEQUENCED ON: | 3/16/2012 |
| EXPECTED MW: | 24kDa |
| OBSERVED MW: | 25kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTDYIRDGQ AIYDRSFAII RAEADLRHIP ADLEKLAVRV IHACGMVDVA NDLAFSEGAG KAGRNALLAG APILCDARMV AEGITRSRLP ADNRVIYTLS DPSVPELAKK IGNTRSAAAL DLWLPHIEGS IVAIGNAPTA LFRLFELLDA GAPKPALIIG MPVGFVGAAE SKDELAANSR GVPYVIVRGR RGGSAMTAAA VNALASERE
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacag actatatccg tgacggacag gccatttacg accgatcctt cgctattatc cgcgccgaag ccgatctccg tcacattcca gccgatcttg aaaagctggc cgttcgcgtg attcacgcct gcggcatggt ggatgtggca aacgatcttg ccttctcgga aggtgcaggc aaagccgggc gcaatgctct tctggcaggc gcgccgatcc tctgcgatgc gcgcatggtg gccgaaggca tcacccgctc gcgccttccc gccgataatc gcgtgatcta caccctgagc gacccatccg tgccggaact tgcaaaaaag atcggcaaca cccgctccgc agccgcgctt gatctctggc tcccgcatat tgaaggcagc attgtcgcca tcggcaatgc gccgaccgcc ctcttccgcc tgttcgagct tctcgatgca ggggcaccga agcccgcact tatcattgga atgccggtgg gctttgtggg agcggcggaa tcgaaggacg agcttgccgc caacagccgc ggcgtgccat atgttatcgt gcgcgggcgg cgcggcggca gcgccatgac ggcagccgcg gtcaacgcgc ttgcttcgga gcgtgaataa taa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDYIRDGQ AIYDRSFAII RAEADLRHIP ADLEKLAVRV IHACGMVDVA NDLAFSEGAG KAGRNALLAG APILCDARMV AEGITRSRLP ADNRVIYTLS DPSVPELAKK IGNTRSAAAL DLWLPHIEGS IVAIGNAPTA LFRLFELLDA GAPKPALIIG MPVGFVGAAE SKDELAANSR GVPYVIVRGR RGGSAMTAAA VNALASERE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacagactat atccgtgacg gacaggccat ttacgaccga tccttcgcta ttatccgcgc cgaagccgat ctccgtcaca ttccagccga tcttgaaaag ctggccgttc gcgtgattca cgcctgcggc atggtggatg tggcaaacga tcttgccttc tcggaaggtg caggcaaagc cgggcgcaat gctcttctgg caggcgcgcc gatcctctgc gatgcgcgca tggtggccga aggcatcacc cgctcgcgcc ttcccgccga taatcgcgtg atctacaccc tgagcgaccc atccgtgccg gaacttgcaa aaaagatcgg caacacccgc tccgcagccg cgcttgatct ctggctcccg catattgaag gcagcattgt cgccatcggc aatgcgccga ccgccctctt ccgcctgttc gagcttctcg atgcaggggc accgaagccc gcacttatca ttggaatgcc ggtgggcttt gtgggagcgg cggaatcgaa ggacgagctt gccgccaaca gccgcggcgt gccatatgtt atcgtgcgcg ggcggcgcgg cggcagcgcc atgacggcag ccgcggtcaa cgcgcttgct tcggagcgtg aataaaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BrabA.00006.a.A1.PS00031
| PURIFICATION DATe: | 6/24/2008 |
| CONCENTRATION: | 19.96mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTDYIRDGQ AIYDRSFAII RAEADLRHIP ADLEKLAVRV IHACGMVDVA NDLAFSEGAG KAGRNALLAG APILCDARMV AEGITRSRLP ADNRVIYTLS DPSVPELAKK IGNTRSAAAL DLWLPHIEGS IVAIGNAPTA LFRLFELLDA GAPKPALIIG MPVGFVGAAE SKDELAANSR GVPYVIVRGR RGGSAMTAAA VNALASERE
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacag actatatccg tgacggacag gccatttacg accgatcctt cgctattatc cgcgccgaag ccgatctccg tcacattcca gccgatcttg aaaagctggc cgttcgcgtg attcacgcct gcggcatggt ggatgtggca aacgatcttg ccttctcgga aggtgcaggc aaagccgggc gcaatgctct tctggcaggc gcgccgatcc tctgcgatgc gcgcatggtg gccgaaggca tcacccgctc gcgccttccc gccgataatc gcgtgatcta caccctgagc gacccatccg tgccggaact tgcaaaaaag atcggcaaca cccgctccgc agccgcgctt gatctctggc tcccgcatat tgaaggcagc attgtcgcca tcggcaatgc gccgaccgcc ctcttccgcc tgttcgagct tctcgatgca ggggcaccga agcccgcact tatcattgga atgccggtgg gctttgtggg agcggcggaa tcgaaggacg agcttgccgc caacagccgc ggcgtgccat atgttatcgt gcgcgggcgg cgcggcggca gcgccatgac ggcagccgcg gtcaacgcgc ttgcttcgga gcgtgaataa taa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDYIRDGQ AIYDRSFAII RAEADLRHIP ADLEKLAVRV IHACGMVDVA NDLAFSEGAG KAGRNALLAG APILCDARMV AEGITRSRLP ADNRVIYTLS DPSVPELAKK IGNTRSAAAL DLWLPHIEGS IVAIGNAPTA LFRLFELLDA GAPKPALIIG MPVGFVGAAE SKDELAANSR GVPYVIVRGR RGGSAMTAAA VNALASERE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACAGACTAT ATCCGTGACG GACAGGCCAT TTACGACCGA TCCTTCGCTA TTATCCGCGC CGAAGCCGAT CTCCGTCACA TTCCAGCCGA TCTTGAAAAG CTGGCCGTTC GCGTGATTCA CGCCTGCGGC ATGGTGGATG TGGCAAACGA TCTTGCCTTC TCGGAAGGTG CAGGCAAAGC CGGGCGCAAT GCTCTTCTGG CAGGCGCGCC GATCCTCTGC GATGCGCGCA TGGTGGCCGA AGGCATCACC CGCTCGCGCC TTCCCGCCGA TAATCGCGTG ATCTACACCC TGAGCGACCC ATCCGTGCCG GAACTTGCAA AAAAGATCGG CAACACCCGC TCCGCAGCCG CGCTTGATCT CTGGCTCCCG CATATTGAAG GCAGCATTGT CGCCATCGGC AATGCGCCGA CCGCCCTCTT CCGCCTGTTC GAGCTTCTCG ATGCAGGGGC ACCGAAGCCC GCACTTATCA TTGGAATGCC GGTGGGCTTT GTGGGAGCGG CGGAATCGAA GGACGAGCTT GCCGCCAACA GCCGCGGCGT GCCATATGTT ATCGTGCGCG GGCGGCGCGG CGGCAGCGCC ATGACGGCAG CCGCGGTCAA CGCGCTTGCT TCGGAGCGTG AATAAAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA