BrabA.00005.a
Lactate/malate dehydrogenase:L-lactate dehydrogenase:TrkA potassium uptake protein
| CENTER ID: | BrabA.00005.a |
| ORGANISM: | Brucella melitensis biovar Abortus 2308 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.00005.a.A1.GE25209 | Full length( BrabA.00005.a ) | 1 | 320 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.00005.a.A1.PW25247 | Full length( BrabA.00005.a ) | 1 | 320 |
Structures
3GVH
DEPOSITED: 3/31/2009
DETERMINATION: XRay
CLONE: BrabA.00005.a.A1.GE25209
PROTEIN: BrabA.00005.a.A1.PW25247
3GVI
DEPOSITED: 3/31/2009
DETERMINATION: XRay
CLONE: BrabA.00005.a.A1.GE25209
PROTEIN: BrabA.00005.a.A1.PW25247
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126911 |
| PATRIC ID: | fig|359391.4.peg.2007 |
| RefSeq: | YP_415266 |
| UniProt: | Q2YLR9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MARNKIALIG SGMIGGTLAH LAGLKELGDV VLFDIAEGTP QGKGLDIAES SPVDGFDAKF TGANDYAAIE GADVVIVTAG VPRKPGMSRD DLLGINLKVM EQVGAGIKKY APEAFVICIT NPLDAMVWAL QKFSGLPAHK VVGMAGVLDS ARFRYFLSEE FNVSVEDVTV FVLGGHGDSM VPLARYSTVA GIPLPDLVKM GWTSQDKLDK IIQRTRDGGA EIVGLLKTGS AFYAPAASAI QMAESYLKDK KRVLPVAAQL SGQYGVKDMY VGVPTVIGAN GVERIIEIDL DKDEKAQFDK SVASVAGLCE ACIGIAPSLK
NT Sequence
ATGGCACGCA ACAAGATTGC CCTCATCGGC TCCGGCATGA TCGGCGGTAC GCTCGCTCAC CTGGCCGGTC TGAAGGAACT CGGTGACGTC GTCCTTTTCG ACATTGCGGA AGGCACCCCG CAGGGCAAGG GGCTGGATAT CGCCGAATCT TCTCCGGTCG ATGGTTTCGA TGCGAAGTTC ACTGGCGCGA ACGATTACGC CGCCATTGAA GGCGCAGACG TCGTCATCGT CACCGCAGGC GTGCCGCGCA AGCCGGGCAT GAGCCGCGAC GATCTCCTGG GCATCAACCT GAAGGTGATG GAACAGGTGG GCGCGGGCAT CAAGAAATAT GCGCCGGAAG CTTTCGTCAT CTGCATCACC AACCCGCTCG ACGCCATGGT CTGGGCGCTG CAGAAGTTCT CCGGCCTTCC GGCCCACAAG GTTGTCGGCA TGGCTGGCGT TCTCGACAGC GCCCGCTTCC GTTATTTCCT CTCGGAAGAA TTCAACGTTT CGGTCGAGGA CGTCACGGTA TTCGTGCTGG GTGGCCACGG CGATTCGATG GTTCCGCTGG CGCGCTACTC GACCGTTGCC GGCATTCCGC TGCCTGATCT CGTCAAGATG GGCTGGACCA GCCAGGACAA GCTCGACAAG ATCATCCAGC GCACCCGTGA CGGCGGCGCG GAAATCGTGG GCCTTCTCAA GACCGGCTCG GCTTTCTACG CTCCGGCGGC ATCGGCCATC CAGATGGCTG AATCCTACCT CAAGGACAAG AAGCGCGTCC TGCCGGTCGC AGCCCAGCTT TCGGGCCAGT ATGGCGTGAA GGACATGTAT GTGGGCGTTC CGACCGTGAT CGGCGCCAAT GGCGTGGAGC GCATCATCGA GATCGATCTC GACAAGGACG AGAAGGCGCA GTTCGACAAG TCGGTGGCCT CCGTCGCCGG TCTGTGCGAA GCCTGCATCG GTATTGCTCC GTCGCTGAAA
Details for BrabA.00005.a.A1.GE25209
| HARVESTED ON: | 11/19/2008 |
| SEQUENCED ON: | 6/16/2009 |
| EXPECTED MW: | 35kDa |
| OBSERVED MW: | 36kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMARNKIALI GSGMIGGTLA HLAGLKELGD VVLFDIAEGT PQGKGLDIAE SSPVDGFDAK FTGANDYAAI EGADVVIVTA GVPRKPGMSR DDLLGINLKV MEQVGAGIKK YAPEAFVICI TNPLDAMVWA LQKFSGLPAH KVVGMAGVLD SARFRYFLSE EFNVSVEDVT VFVLGGHGDS MVPLARYSTV AGIPLPDLVK MGWTSQDKLD KIIQRTRDGG AEIVGLLKTG SAFYAPAASA IQMAESYLKD KKRVLPVAAQ LSGQYGVKDM YVGVPTVIGA NGVERIIEID LDKDEKAQFD KSVASVAGLC EACIGIAPSL K
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcac gcaacaagat tgccctcatc ggctccggca tgatcggcgg tacgctcgct cacctggccg gtctgaagga actcggtgac gtcgtccttt tcgacattgc ggaaggcacc ccgcagggca aggggctgga tatcgccgaa tcttctccgg tcgatggttt cgatgcgaag ttcactggcg cgaacgatta cgccgccatt gaaggcgcag acgtcgtcat cgtcaccgca ggcgtgccgc gcaagccggg catgagccgc gacgatctcc tgggcatcaa cctgaaggtg atggaacagg tgggcgcggg catcaagaaa tatgcgccgg aagcattcgt catctgcatc accaacccgc tcgacgccat ggtctgggcg ctgcagaagt tctccggcct tccggcccac aaggttgtcg gcatggctgg cgttctcgac agcgcccgct tccgttattt cctctcggaa gaattcaacg tttcggtcga ggacgtcacg gtattcgtgc tgggtggcca cggcgattcg atggttccgc tggcgcgcta ctcgaccgtt gccggcattc cgctgcctga tctcgtcaag atgggctgga ccagccagga caagctcgac aagatcatcc agcgcacccg tgacggcggc gcggaaatcg tgggccttct caagaccggc tcggctttct acgctccggc ggcatcggcc atccagatgg ctgaatccta cctcaaggac aagaagcgcg tcctgccggt cgcagcccag ctttcgggcc agtatggcgt gaaggacatg tatgtgggcg ttccgaccgt gatcggtgcc aatggcgtgg agcgcatcat cgagatcgat ctcgacaagg acgagaaggc gcagttcgac aagtcggtgg cctccgtcgc cggtctgtgc gaagcctgca tcggtattgc tccgtcgctg aaataataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMARNKIALI GSGMIGGTLA HLAGLKELGD VVLFDIAEGT PQGKGLDIAE SSPVDGFDAK FTGANDYAAI EGADVVIVTA GVPRKPGMSR DDLLGINLKV MEQVGAGIKK YAPEAFVICI TNPLDAMVWA LQKFSGLPAH KVVGMAGVLD SARFRYFLSE EFNVSVEDVT VFVLGGHGDS MVPLARYSTV AGIPLPDLVK MGWTSQDKLD KIIQRTRDGG AEIVGLLKTG SAFYAPAASA IQMAESYLKD KKRVLPVAAQ LSGQYGVKDM YVGVPTVIGA NGVERIIEID LDKDEKAQFD KSVASVAGLC EACIGIAPSL K
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcacgcaac aagattgccc tcatcggctc cggcatgatc ggcggtacgc tcgctcacct ggccggtctg aaggaactcg gtgacgtcgt ccttttcgac attgcggaag gcaccccgca gggcaagggg ctggatatcg ccgaatcttc tccggtcgat ggtttcgatg cgaagttcac tggcgcgaac gattacgccg ccattgaagg cgcagacgtc gtcatcgtca ccgcaggcgt gccgcgcaag ccgggcatga gccgcgacga tctcctgggc atcaacctga aggtgatgga acaggtgggc gcgggcatca agaaatatgc gccggaagca ttcgtcatct gcatcaccaa cccgctcgac gccatggtct gggcgctgca gaagttctcc ggccttccgg cccacaaggt tgtcggcatg gctggcgttc tcgacagcgc ccgcttccgt tatttcctct cggaagaatt caacgtttcg gtcgaggacg tcacggtatt cgtgctgggt ggccacggcg attcgatggt tccgctggcg cgctactcga ccgttgccgg cattccgctg cctgatctcg tcaagatggg ctggaccagc caggacaagc tcgacaagat catccagcgc acccgtgacg gcggcgcgga aatcgtgggc cttctcaaga ccggctcggc tttctacgct ccggcggcat cggccatcca gatggctgaa tcctacctca aggacaagaa gcgcgtcctg ccggtcgcag cccagctttc gggccagtat ggcgtgaagg acatgtatgt gggcgttccg accgtgatcg gtgccaatgg cgtggagcgc atcatcgaga tcgatctcga caaggacgag aaggcgcagt tcgacaagtc ggtggcctcc gtcgccggtc tgtgcgaagc ctgcatcggt attgctccgt cgctgaaata aaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for BrabA.00005.a.A1.PW25247
| PURIFICATION DATe: | 6/12/2008 |
| CONCENTRATION: | 28.5mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 24 |
| VIAL VOLUME: | 50µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMARNKIALI GSGMIGGTLA HLAGLKELGD VVLFDIAEGT PQGKGLDIAE SSPVDGFDAK FTGANDYAAI EGADVVIVTA GVPRKPGMSR DDLLGINLKV MEQVGAGIKK YAPEAFVICI TNPLDAMVWA LQKFSGLPAH KVVGMAGVLD SARFRYFLSE EFNVSVEDVT VFVLGGHGDS MVPLARYSTV AGIPLPDLVK MGWTSQDKLD KIIQRTRDGG AEIVGLLKTG SAFYAPAASA IQMAESYLKD KKRVLPVAAQ LSGQYGVKDM YVGVPTVIGA NGVERIIEID LDKDEKAQFD KSVASVAGLC EACIGIAPSL K
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcac gcaacaagat tgccctcatc ggctccggca tgatcggcgg tacgctcgct cacctggccg gtctgaagga actcggtgac gtcgtccttt tcgacattgc ggaaggcacc ccgcagggca aggggctgga tatcgccgaa tcttctccgg tcgatggttt cgatgcgaag ttcactggcg cgaacgatta cgccgccatt gaaggcgcag acgtcgtcat cgtcaccgca ggcgtgccgc gcaagccggg catgagccgc gacgatctcc tgggcatcaa cctgaaggtg atggaacagg tgggcgcggg catcaagaaa tatgcgccgg aagcattcgt catctgcatc accaacccgc tcgacgccat ggtctgggcg ctgcagaagt tctccggcct tccggcccac aaggttgtcg gcatggctgg cgttctcgac agcgcccgct tccgttattt cctctcggaa gaattcaacg tttcggtcga ggacgtcacg gtattcgtgc tgggtggcca cggcgattcg atggttccgc tggcgcgcta ctcgaccgtt gccggcattc cgctgcctga tctcgtcaag atgggctgga ccagccagga caagctcgac aagatcatcc agcgcacccg tgacggcggc gcggaaatcg tgggccttct caagaccggc tcggctttct acgctccggc ggcatcggcc atccagatgg ctgaatccta cctcaaggac aagaagcgcg tcctgccggt cgcagcccag ctttcgggcc agtatggcgt gaaggacatg tatgtgggcg ttccgaccgt gatcggtgcc aatggcgtgg agcgcatcat cgagatcgat ctcgacaagg acgagaaggc gcagttcgac aagtcggtgg cctccgtcgc cggtctgtgc gaagcctgca tcggtattgc tccgtcgctg aaataataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMARNKIALI GSGMIGGTLA HLAGLKELGD VVLFDIAEGT PQGKGLDIAE SSPVDGFDAK FTGANDYAAI EGADVVIVTA GVPRKPGMSR DDLLGINLKV MEQVGAGIKK YAPEAFVICI TNPLDAMVWA LQKFSGLPAH KVVGMAGVLD SARFRYFLSE EFNVSVEDVT VFVLGGHGDS MVPLARYSTV AGIPLPDLVK MGWTSQDKLD KIIQRTRDGG AEIVGLLKTG SAFYAPAASA IQMAESYLKD KKRVLPVAAQ LSGQYGVKDM YVGVPTVIGA NGVERIIEID LDKDEKAQFD KSVASVAGLC EACIGIAPSL K
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCACGCAAC AAGATTGCCC TCATCGGCTC CGGCATGATC GGCGGTACGC TCGCTCACCT GGCCGGTCTG AAGGAACTCG GTGACGTCGT CCTTTTCGAC ATTGCGGAAG GCACCCCGCA GGGCAAGGGG CTGGATATCG CCGAATCTTC TCCGGTCGAT GGTTTCGATG CGAAGTTCAC TGGCGCGAAC GATTACGCCG CCATTGAAGG CGCAGACGTC GTCATCGTCA CCGCAGGCGT GCCGCGCAAG CCGGGCATGA GCCGCGACGA TCTCCTGGGC ATCAACCTGA AGGTGATGGA ACAGGTGGGC GCGGGCATCA AGAAATATGC GCCGGAAGCA TTCGTCATCT GCATCACCAA CCCGCTCGAC GCCATGGTCT GGGCGCTGCA GAAGTTCTCC GGCCTTCCGG CCCACAAGGT TGTCGGCATG GCTGGCGTTC TCGACAGCGC CCGCTTCCGT TATTTCCTCT CGGAAGAATT CAACGTTTCG GTCGAGGACG TCACGGTATT CGTGCTGGGT GGCCACGGCG ATTCGATGGT TCCGCTGGCG CGCTACTCGA CCGTTGCCGG CATTCCGCTG CCTGATCTCG TCAAGATGGG CTGGACCAGC CAGGACAAGC TCGACAAGAT CATCCAGCGC ACCCGTGACG GCGGCGCGGA AATCGTGGGC CTTCTCAAGA CCGGCTCGGC TTTCTACGCT CCGGCGGCAT CGGCCATCCA GATGGCTGAA TCCTACCTCA AGGACAAGAA GCGCGTCCTG CCGGTCGCAG CCCAGCTTTC GGGCCAGTAT GGCGTGAAGG ACATGTATGT GGGCGTTCCG ACCGTGATCG GTGCCAATGG CGTGGAGCGC ATCATCGAGA TCGATCTCGA CAAGGACGAG AAGGCGCAGT TCGACAAGTC GGTGGCCTCC GTCGCCGGTC TGTGCGAAGC CTGCATCGGT ATTGCTCCGT CGCTGAAATA AAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA