BrabA.00002.a
Bacterial transferase hexapeptide repeat:2,3,4,5-tetrahydropyridine-2-carboxylate N-succinyltransferase
| CENTER ID: | BrabA.00002.a |
| ORGANISM: | Brucella melitensis biovar Abortus 2308 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrabA.00002.a.A1.GE25207 | Full length( BrabA.00002.a ) | 1 | 284 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
3EG4
DEPOSITED: 9/10/2008
DETERMINATION: XRay
CLONE: BrabA.00002.a.A1.GE25207
PROTEIN: BrabA.00002.a.A1.PW25245
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|359391.4.peg.3288 |
| RefSeq: | YP_419127 |
| UniProt: | Q2YJQ7 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTKPDLASLE KTIEKAFDER DGINTATRGE VREAVEQSLI LLDRGEVRVA EKQADGNWHV NQWLKKAVLL SFRLNPMEVI KGGPGQSSWW DKVPSKFDGW TANEFEKAGF RAVPNCIVRH SAYIAPNAIL MPSFVNLGAY VDKGAMIDTW ATVGSCAQIG KNVHLSGGVG IGGVLEPMQA GPTIIEDNCF IGARSEVVEG CIVREGSVLG MGVFIGKSTK IVDRATGEVF YGEVPPYSVV VAGTMPGKNV PGENWGPSLY CAVIVKRADE KTRSKTSINE LLRD
NT Sequence
ATGACCAAGC CAGACCTCGC CTCCCTTGAG AAGACAATCG AAAAGGCTTT TGACGAGCGC GACGGTATCA ACACGGCCAC GCGCGGCGAA GTGCGCGAAG CCGTCGAACA GTCGCTCATT CTGCTCGACC GCGGTGAAGT CCGTGTCGCA GAAAAACAGG CGGACGGCAA CTGGCATGTC AACCAATGGC TGAAGAAAGC GGTTCTGCTC TCCTTCCGCC TGAACCCGAT GGAAGTCATC AAGGGCGGCC CCGGCCAGTC CTCCTGGTGG GACAAGGTTC CCTCCAAGTT CGATGGCTGG ACGGCAAACG AATTTGAAAA GGCGGGCTTC CGCGCGGTTC CCAACTGCAT CGTGCGCCAC TCGGCCTATA TCGCGCCCAA TGCCATCCTG ATGCCCTCCT TCGTCAATCT CGGCGCCTAT GTCGATAAAG GCGCCATGAT CGATACATGG GCAACCGTCG GCTCCTGCGC ACAGATTGGC AAAAACGTGC ACCTTTCCGG CGGCGTCGGC ATTGGCGGCG TTCTGGAACC CATGCAGGCG GGCCCCACCA TTATTGAAGA CAACTGCTTC ATCGGCGCGC GTTCGGAAGT GGTCGAAGGC TGCATCGTGC GCGAAGGCTC GGTGCTCGGC ATGGGCGTCT TCATTGGCAA ATCCACCAAG ATCGTGGACC GCGCAACCGG AGAGGTCTTC TATGGCGAAG TGCCTCCCTA TTCGGTCGTG GTTGCTGGCA CCATGCCGGG CAAGAACGTC CCCGGCGAAA ACTGGGGGCC GAGCCTTTAT TGCGCCGTGA TTGTCAAACG CGCGGACGAG AAGACCCGTT CCAAGACCTC GATCAACGAA TTGCTGCGCG AC
Details for BrabA.00002.a.A1.GE25207
| HARVESTED ON: | 5/6/2008 |
| SEQUENCED ON: | 7/29/2008 |
| EXPECTED MW: | 33kDa |
| OBSERVED MW: | 33kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MGTLEAQTQG PGSMTKPDLA SLEKTIEKAF DERDGINTAT RGEVREAVEQ SLILLDRGEV RVAEKQADGN WHVNQWLKKA VLLSFRLNPM EVIKGGPGQS SWWDKVPSKF DGWTANEFEK AGFRAVPNCI VRHSAYIAPN AILMPSFVNL GAYVDKGAMI DTWATVGSCA QIGKNVHLSG GVGIGGVLEP MQAGPTIIED NCFIGARSEV VEGCIVREGS VLGMGVFIGK STKIVDRATG EVFYGEVPPY SVVVAGTMPG KNVPGENWGP SLYCAVIVKR ADEKTRSKTS INELLRD
Validated NT Sequence
tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccaagcca gacctcgcct cccttgagaa gacaatcgaa aaggcttttg acgagcgcga cggtatcaac acggccacgc gcggcgaagt gcgcgaagcc gtcgaacagt cgctcattct gctcgaccgc ggtgaagtcc gtgtcgcaga aaaacaggcg gacggcaact ggcatgtcaa ccaatggctg aagaaagcgg ttctgctctc cttccgcctg aacccgatgg aagtcatcaa gggcggcccc ggccagtcct cctggtggga caaggttccc tccaagttcg atggctggac ggcaaacgaa tttgaaaagg cgggcttccg cgcggttccc aactgcatcg tgcgccactc ggcctatatc gcgcccaatg ccatcctgat gccctccttc gtcaatctcg gtgcctatgt cgataaaggc gccatgatcg atacatgggc aaccgtcggc tcctgcgcac agattggcaa aaacgtgcac ctttccggcg gcgtcggcat tggcggcgtt ctggaaccca tgcaggcggg ccccaccatt attgaagaca actgcttcat cggcgcgcgt tcggaagtgg tcgaaggctg catcgtgcgc gaaggctcgg tgctcggcat gggcgtcttc attggcaaat ccaccaagat cgtggaccgc gcaaccggag aggtcttcta tggcgaagtg cctccctatt cggtcgtggt tgctggcacc atgccgggca agaacgtccc cggcgaaaac tgggggccga gcctttattg cgccgtgatt gtcaaacgcg cggacgagaa gacccgttcc aagacctcga tcaacgaatt gctgcgcgac taataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTKPDLASL EKTIEKAFDE RDGINTATRG EVREAVEQSL ILLDRGEVRV AEKQADGNWH VNQWLKKAVL LSFRLNPMEV IKGGPGQSSW WDKVPSKFDG WTANEFEKAG FRAVPNCIVR HSAYIAPNAI LMPSFVNLGA YVDKGAMIDT WATVGSCAQI GKNVHLSGGV GIGGVLEPMQ AGPTIIEDNC FIGARSEVVE GCIVREGSVL GMGVFIGKST KIVDRATGEV FYGEVPPYSV VVAGTMPGKN VPGENWGPSL YCAVIVKRAD EKTRSKTSIN ELLRD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccaagcca gacctcgcct cccttgagaa gacaatcgaa aaggcttttg acgagcgcga cggtatcaac acggccacgc gcggcgaagt gcgcgaagcc gtcgaacagt cgctcattct gctcgaccgc ggtgaagtcc gtgtcgcaga aaaacaggcg gacggcaact ggcatgtcaa ccaatggctg aagaaagcgg ttctgctctc cttccgcctg aacccgatgg aagtcatcaa gggcggcccc ggccagtcct cctggtggga caaggttccc tccaagttcg atggctggac ggcaaacgaa tttgaaaagg cgggcttccg cgcggttccc aactgcatcg tgcgccactc ggcctatatc gcgcccaatg ccatcctgat gccctccttc gtcaatctcg gtgcctatgt cgataaaggc gccatgatcg atacatgggc aaccgtcggc tcctgcgcac agattggcaa aaacgtgcac ctttccggcg gcgtcggcat tggcggcgtt ctggaaccca tgcaggcggg ccccaccatt attgaagaca actgcttcat cggcgcgcgt tcggaagtgg tcgaaggctg catcgtgcgc gaaggctcgg tgctcggcat gggcgtcttc attggcaaat ccaccaagat cgtggaccgc gcaaccggag aggtcttcta tggcgaagtg cctccctatt cggtcgtggt tgctggcacc atgccgggca agaacgtccc cggcgaaaac tgggggccga gcctttattg cgccgtgatt gtcaaacgcg cggacgagaa gacccgttcc aagacctcga tcaacgaatt gctgcgcgac tgaaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa