BotuA.20234.a

Uncharacterized protein

CENTER ID: BotuA.20234.a
ORGANISM: Borrelia turicatae
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BotuA.20234.a.B2.GE42728 mature protein 26 487
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
UniProt: T1ECR6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLKVRYRNSL LVLILLLLLV MGCNFKSPKD AAASGENSIV KKNEKDPKEH DTESLNAKLD NLLNTFGISE AGKRSIVRIK DVVTDVGIGS VEGYRNYTDS ELYALLNDLG AAKIQEIIKA DLELVKTQKT ALEAINNVHK PKERQNLQLR FNSKRNEYPL HLKGLFNESD FNVVYSNVIG DNYVNEFTAI KEEIIQVIGD FDLYKGLSSR EKSVVDYIQS VVTNANFCYA NNDCKTYTLT EFYALLNSLG DAKIREMIKV NLDVFKVREE IEADIAGLTE EKVKQALKTR LASYNNEYAF ILRGAFNASG ADNIYSRFMK INYINAYINL KKKIRDPRVL DVYTWLSDED KGVIDKIQGI VTDSNIGHAK GYKTYTDSEF NDLLNNLGAS KTRSMITAFL RANVLREMAL EAINTVPAGT AKQNFQNRFN ALSNGYALRL KGLFNSLNHD MIYDSVVNDE YSEKFLRIKD DIRHLSSNTA DSTASSN
NT Sequence
atgttaaagg taagatatcg taattcgtta ttagtattga tattgctatt attactagta atgggttgta atttcaaaag tccaaaagac gctgctgcga gtggggaaaa ctcgattgta aaaaaaaatg aaaaagaccc caaagagcat gatacagaga gtttaaatgc caagcttgat aatcttctaa acacatttgg aatatctgag gcaggcaaga gatcaattgt taggattaaa gatgtggtaa ctgatgttgg tattggtagt gttgaaggtt acaggaatta tactgattct gagctttatg ctttgttaaa tgacttaggt gctgctaaga ttcaagagat tataaaagcc gatttagagc ttgttaaaac ccaaaaaaca gctcttgagg ctataaataa tgttcataaa cctaaagaaa gacaaaattt gcaacttagg tttaatagta agagaaatga gtacccattg catttaaaag ggttatttaa tgaatctgat tttaatgttg tgtatagcaa tgttataggt gataactatg ttaatgaatt tactgcgatt aaagaagaaa ttattcaagt tataggagac tttgatttat ataaagggct ttcatctcgg gaaaagtccg tagttgatta tatacaaagt gtagtaacta atgctaactt ttgttatgct aataacgatt gcaagactta tactcttact gagttttatg ctttgttaaa tagcttaggt gatgctaaga ttagggaaat gataaaagtt aatttagatg tcttcaaagt tcgagaggaa attgaggcag atattgcagg gctaactgaa gagaaagtaa aacaagcatt gaagactaga cttgctagtt ataacaatga gtatgcattt attttaagag gggcttttaa tgcatctggg gcggataata tatattctcg ttttatgaag attaattata ttaatgctta tattaattta aaaaagaaaa tcagagatcc acgggtttta gatgtataca catggctttc tgatgaggat aaaggcgtaa ttgataaaat acaaggtata gtaactgatt ctaatattgg tcatgctaaa ggttacaaga cttatactga ttctgagttt aatgatttgt taaataactt aggtgcgtct aagactagga gtatgataac agctttttta agggctaacg tccttcggga aatggctttg gaggctataa atactgttcc tgcaggtaca gcaaaacaaa actttcaaaa tagatttaat gcactaagta atggatatgc gttgcgttta aaagggttat ttaacagctt aaatcatgat atgatttatg atagtgttgt aaatgatgaa tattctgaaa aatttcttag gattaaagat gacattagac acctttcatc aaatacagca gatagtaccg caagtagtaa ttaa
Details for BotuA.20234.a.B2.GE42728
HARVESTED ON: 11/5/2018
SEQUENCED ON: 11/7/2018
EXPECTED MW: 53kDa
OBSERVED MW: 53kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 96
PERCENT COVERAGE: 42
Validated AA Sequence
MAHHHHHHMK SPKDAAASGE NSIVKKNEKD PKEHDTESLN AKLDNLLNTF GISEAGKRSI VRIKDVVTDV GIGSVEGYRN YTDSELYALL NDLGAAKIQE IIKADLELVK TQKTALEAIN NVHKPKERQN LQLRFNSKRN EYPLHLKGLF NESDFNVVYS NVIGDNYVNE FTAIKEEIIQ VXXDFDLYKX XFISGKVRS
Validated NT Sequence
ctgctgtatt tgatgaaagg tgtctaatgt catctttaat cctaagaaat ttttcagaat attcatcatt tacaacacta tcataaatca tatcatgatt taagctgtta aataaccctt ttaaacgcaa cgcatatcca ttacttagtg cattaaatct attttgaaag ttttgttttg ctgtacctgc aggaacagta tttatagcct ccaaagccat ttcccgaagg acgttagccc ttaaaaaagc tgttatcata ctcctagtct tagacgcacc taagttattt aacaaatcat taaactcaga atcagtataa gtcttgtaac ctttagcatg accaatatta gaatcagtta ctataccttg tattttatca attacgcctt tatcctcatc agaaagccat gtgtatacat ctaaaacccg tggatctctg attttctttt ttaaattaat ataagcatta atataattaa tcttcataaa acgagaatat atattatccg ccccagatgc attaaaagcc cctcttaaaa taaatgcata ctcattgtta taactagcaa gtctagtctt caatgcttgt tttactttct cttcagttag ccctgcaata tctgcctcaa tttcctctcg aactttgaag acatctaaat taacttttat catttcccta atcttagcat cacctannnt atttaacaaa gcataaaact cagtaagagt ataagtcttg caatcgttat tagcataaca aaaagttagc attagttact acactttgta tataatcaac tacggacttt tcccgagatg aaannncttt tatataaatc aaagtcnnnn nnaacttgaa taatttcttc tttaatcgca gtaaattcat taacatagtt atcacctata acattgctat acacaacatt aaaatcagat tcattaaata acccttttaa atgcaatggg tactcatttc tcttactatt aaacctaagt tgcaaatttt gtctttcttt aggtttatga acattattta tagcctcaag agctgttttt tgggttttaa caagctctaa atcggctttt ataatctctt gaatcttagc agcacctaag tcatttaaca aagcataaag ctcagaatca gtataattcc tgtaaccttc aacactacca ataccaacat cagttaccac atctttaatc ctaacaattg atctcttgcc tgcctcagat attccaaatg tgtttagaag attatcaagc ttggcattta aactctctgt atcatgctct ttggggtctt tttcattttt ttttacaatc gagttttccc cactcgcagc agcgtctttt ggacttttca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHKS PKDAAASGEN SIVKKNEKDP KEHDTESLNA KLDNLLNTFG ISEAGKRSIV RIKDVVTDVG IGSVEGYRNY TDSELYALLN DLGAAKIQEI IKADLELVKT QKTALEAINN VHKPKERQNL QLRFNSKRNE YPLHLKGLFN ESDFNVVYSN VIGDNYVNEF TAIKEEIIQV IGDFDLYKGL SSREKSVVDY IQSVVTNANF CYANNDCKTY TLTEFYALLN SLGDAKIREM IKVNLDVFKV REEIEADIAG LTEEKVKQAL KTRLASYNNE YAFILRGAFN ASGADNIYSR FMKINYINAY INLKKKIRDP RVLDVYTWLS DEDKGVIDKI QGIVTDSNIG HAKGYKTYTD SEFNDLLNNL GASKTRSMIT AFLRANVLRE MALEAINTVP AGTAKQNFQN RFNALSNGYA LRLKGLFNSL NHDMIYDSVV NDEYSEKFLR IKDDIRHLSS NTADSTASSN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataaaagtc caaaagacgc tgctgcgagt ggggaaaact cgattgtaaa aaaaaatgaa aaagacccca aagagcatga tacagagagt ttaaatgcca agcttgataa tcttctaaac acatttggaa tatctgaggc aggcaagaga tcaattgtta ggattaaaga tgtggtaact gatgttggta ttggtagtgt tgaaggttac aggaattata ctgattctga gctttatgct ttgttaaatg acttaggtgc tgctaagatt caagagatta taaaagccga tttagagctt gttaaaaccc aaaaaacagc tcttgaggct ataaataatg ttcataaacc taaagaaaga caaaatttgc aacttaggtt taatagtaag agaaatgagt acccattgca tttaaaaggg ttatttaatg aatctgattt taatgttgtg tatagcaatg ttataggtga taactatgtt aatgaattta ctgcgattaa agaagaaatt attcaagtta taggagactt tgatttatat aaagggcttt catctcggga aaagtccgta gttgattata tacaaagtgt agtaactaat gctaactttt gttatgctaa taacgattgc aagacttata ctcttactga gttttatgct ttgttaaata gcttaggtga tgctaagatt agggaaatga taaaagttaa tttagatgtc ttcaaagttc gagaggaaat tgaggcagat attgcagggc taactgaaga gaaagtaaaa caagcattga agactagact tgctagttat aacaatgagt atgcatttat tttaagaggg gcttttaatg catctggggc ggataatata tattctcgtt ttatgaagat taattatatt aatgcttata ttaatttaaa aaagaaaatc agagatccac gggttttaga tgtatacaca tggctttctg atgaggataa aggcgtaatt gataaaatac aaggtatagt aactgattct aatattggtc atgctaaagg ttacaagact tatactgatt ctgagtttaa tgatttgtta aataacttag gtgcgtctaa gactaggagt atgataacag cttttttaag ggctaacgtc cttcgggaaa tggctttgga ggctataaat actgttcctg caggtacagc aaaacaaaac tttcaaaata gatttaatgc actaagtaat ggatatgcgt tgcgtttaaa agggttattt aacagcttaa atcatgatat gatttatgat agtgttgtaa atgatgaata ttctgaaaaa tttcttagga ttaaagatga cattagacac ctttcatcaa atacagcaga tagtaccgca agtagtaatt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat