BopeA.10201.a

Methionine--tRNA ligase

CENTER ID: BopeA.10201.a
ORGANISM: Bordetella pertussis Tohama I
ASSOCIATED DISEASE:
CURRENT STATUS: in PDB
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BopeA.10201.a.B2.PW39542 AlphaFold2 domain 572 692

Structures

38LI
DEPOSITED: 9/1/2026
DETERMINATION: XRay
CLONE: BopeA.10201.a.B2.GE45700
PROTEIN: BopeA.10201.a.B2.PW39542

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|257313.5.peg.190
UniProt: Q7W0F7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRTLFVTTA LPYANGSFHI GHIMEYIQAD IWVRSMRMAG HTVHFVGADD AHGAPIMLKA EKEGITPQAL VARYAAERPR YLDGFHIRFD HWHSTDTPEN VALSQEIYRA LKSEGLIETR SIEQFYDPVK GMFLADRYIK GECPRCHAKD QYGDSCEVCG AVYAPTELIN PYSALTGAAP VLKSSDHFFF KLSDPRCVEF LQQWTTGANR QGVKHLQAEV QAKTREWLVG DDGEAKLGDW DISRDAPYFG IEIPDAPGKY FYVWLDAPVG YLASLKSYCA AKGLDFDALL DPAGPTEQVH FIGKDIIYFH ALFWPAMLKF AGRKTPDQLN VHGFITVSGE KMSKSRGTGI SPLRYLEIGM DAEWLRYYMA AKLNARVEDM DFNPEDFVAR VNSDLVGKYV NIASRAAAFI TRHFDGELAY DGDTDALAAE FAQQAESIRA AFEAREYNRA VREIMAHADR INQAFDAAQP WVMAKGIGAA DAATRARLQD ICSRALAGFK ALSVMLAPVL PALASRVARE LFGANADFAW GDAQQLPQRV APFKHLMQRV DPKLLDDLFE PPAAEASAPA ALPGGEALAD TITIDDFAKI DLRIARIVNC EEVEGSTKLL RLTLDVGEGR HRNVFSGIKS AYQPQDLVGK LTVLVANLAP RKMKFGVSEG MVLAASHADE KAEPGIYVLE PWPGAQPGMR VR
NT Sequence
ATGTCACGCA CGCTCTTCGT CACCACTGCC CTGCCCTACG CCAACGGATC GTTCCACATC GGCCACATCA TGGAGTACAT CCAGGCCGAC ATCTGGGTCC GTTCGATGCG CATGGCGGGC CACACGGTGC ATTTCGTGGG TGCCGACGAC GCGCACGGCG CGCCGATCAT GCTCAAGGCC GAAAAGGAAG GCATCACGCC GCAGGCGCTG GTGGCCCGCT ACGCCGCCGA GCGGCCGCGC TACCTGGACG GCTTCCATAT CCGCTTCGAC CACTGGCACA GCACCGATAC GCCCGAGAAC GTGGCGCTCT CGCAGGAAAT CTATCGCGCG CTCAAGAGCG AGGGACTGAT CGAGACACGC TCGATCGAGC AGTTCTACGA CCCGGTCAAG GGCATGTTCC TGGCCGACCG CTACATCAAG GGCGAATGCC CGCGCTGCCA CGCCAAGGAC CAGTACGGCG ATTCGTGTGA AGTGTGCGGC GCCGTGTATG CGCCCACCGA GCTGATCAAC CCCTATTCGG CCCTGACCGG CGCCGCGCCG GTACTGAAGT CGTCCGATCA CTTCTTCTTC AAGCTGTCCG ATCCGCGCTG CGTCGAGTTC CTGCAGCAAT GGACCACCGG CGCCAACCGC CAGGGCGTCA AGCACCTGCA GGCCGAAGTG CAGGCCAAGA CGCGCGAATG GCTGGTCGGC GACGACGGCG AGGCCAAGCT GGGCGACTGG GATATCTCGC GCGACGCCCC CTATTTCGGC ATCGAGATTC CCGACGCGCC GGGCAAGTAT TTCTATGTCT GGCTGGACGC GCCGGTGGGC TACCTGGCCT CGCTGAAATC CTATTGCGCG GCCAAGGGCC TGGACTTCGA CGCGCTGCTC GACCCGGCCG GCCCGACCGA GCAGGTGCAC TTCATCGGCA AGGACATCAT TTACTTCCAC GCGCTGTTCT GGCCGGCGAT GCTGAAGTTC GCCGGGCGCA AGACGCCCGA CCAGCTGAAT GTGCACGGTT TCATCACCGT CAGCGGCGAG AAGATGTCCA AGAGCCGCGG CACCGGCATC TCGCCGCTGC GCTACCTGGA AATCGGCATG GACGCCGAAT GGCTGCGCTA CTACATGGCG GCCAAGCTGA ACGCGCGCGT CGAGGACATG GACTTCAACC CCGAAGACTT CGTGGCGCGT GTCAACAGCG ACCTGGTGGG CAAGTACGTC AACATCGCCA GCCGCGCCGC CGCCTTCATT ACCCGCCACT TCGACGGCGA ACTGGCGTAC GACGGCGACA CGGACGCGCT GGCGGCCGAG TTCGCGCAGC AGGCCGAATC GATCCGCGCC GCCTTCGAGG CGCGCGAGTA CAACCGCGCG GTGCGCGAAA TCATGGCCCA TGCCGACCGC ATCAACCAGG CGTTCGACGC GGCGCAGCCC TGGGTGATGG CCAAGGGCAT CGGCGCCGCC GACGCGGCCA CGCGCGCCCG CCTGCAGGAC ATCTGCTCGC GCGCGCTGGC CGGCTTCAAG GCCCTGTCGG TGATGCTGGC GCCGGTGCTG CCCGCGCTGG CCAGCCGCGT GGCGCGCGAG CTGTTCGGCG CGAACGCCGA CTTCGCCTGG GGCGACGCGC AACAGCTGCC GCAGCGGGTG GCGCCGTTCA AGCACCTGAT GCAGCGCGTC GATCCCAAGC TGCTGGACGA CCTGTTCGAG CCGCCCGCCG CCGAAGCCAG CGCGCCGGCC GCCCTGCCGG GCGGCGAGGC CCTGGCCGAC ACCATCACCA TCGACGACTT CGCCAAGATC GACCTGCGCA TCGCGCGCAT CGTCAATTGC GAAGAAGTGG AAGGCTCGAC CAAGCTGCTG CGCCTGACCC TGGACGTGGG CGAGGGACGC CATCGCAATG TGTTCTCGGG CATCAAGTCG GCCTACCAGC CGCAGGACCT GGTCGGCAAG CTGACGGTGC TGGTGGCCAA CCTGGCGCCG CGCAAGATGA AATTCGGCGT GTCCGAGGGC ATGGTGCTGG CCGCCAGCCA CGCCGACGAG AAGGCCGAAC CCGGGATCTA CGTGCTCGAA CCCTGGCCGG GCGCCCAGCC CGGCATGCGG GTGCGC
Details for BopeA.10201.a.B2.PW39542
PURIFICATION DATe: 2/15/2026
CONCENTRATION: 17.4mg/ml
OBSERVED MW: 14kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 17
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHLP GGEALADTIT IDDFAKIDLR IARIVNCEEV EGSTKLLRLT LDVGEGRHRN VFSGIKSAYQ PQDLVGKLTV LVANLAPRKM KFGVSEGMVL AASHADEKAE PGIYVLEPWP GAQPGMRVR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATCTGCCGG GCGGCGAGGC CCTGGCCGAC ACCATCACCA TCGACGACTT CGCCAAGATC GACCTGCGCA TCGCGCGCAT CGTCAATTGC GAAGAAGTGG AAGGCTCGAC CAAGCTGCTG CGCCTGACCC TGGACGTGGG CGAGGGACGC CATCGCAATG TGTTCTCGGG CATCAAGTCG GCCTACCAGC CGCAGGACCT GGTCGGCAAG CTGACGGTGC TGGTGGCCAA CCTGGCGCCG CGCAAGATGA AATTCGGCGT GTCCGAGGGC ATGGTGCTGG CCGCCAGCCA CGCCGACGAG AAGGCCGAAC CCGGGATCTA CGTGCTCGAA CCCTGGCCGG GCGCCCAGCC CGGCATGCGG GTGCGCTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T