SSGCID: Target Page

BopeA.00085.a

Capsular polysaccharide biosynthesis protein

CENTER ID:	BopeA.00085.a
ORGANISM:	Bordetella pertussis Tohama I
ASSOCIATED DISEASE:
CURRENT STATUS:	in PDB
COMMUNITY REQUEST:	False
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

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Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BopeA.00085.a.B2.PW39367	AlphaFold2 domain		2	341

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|257313.5.peg.1749
UniProt:	Q7TTK0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTNRFETTCA QLRAQPQKWL VTGCAGFIGS NLLETLLGLD QAVVGLDNFA TGHQHNLDEV RAAVTPEQWA RFTFIEGDIR DLAACQRAVQ GVDRVLHQAA LGSVPRSLKD PITTNEVNIG GFLNMLVAAR DAQVQAFVYA ASSSTYGDHP DLPKVEERIG NPLSPYAVTK YVNELYADVF ARSYGFSSVG LRYFNVFGKR QDPDGAYAAV IPKWTAAMIK GEDVVINGDG QTSRDFCFVE NAVQANLLAA MAAPEGANQV YNVAYNARTT LTELFEHLRR TLAGQGVSYE KAPVYAEFRA GDVRHSQADI GKAGKLLGYE PAYDILRGLE AAMPWYTQFL R

NT Sequence

ATGACGAATC GCTTTGAGAC CACGTGCGCG CAGTTGCGGG CCCAACCCCA GAAATGGCTG GTGACCGGCT GCGCCGGCTT CATCGGCTCG AACCTGCTCG AGACGTTGCT CGGGCTGGAC CAGGCGGTGG TGGGGCTGGA CAACTTCGCC ACGGGACACC AGCACAACCT GGACGAGGTG CGCGCGGCGG TGACGCCCGA GCAATGGGCG CGCTTCACCT TCATCGAAGG CGACATCCGC GACCTGGCGG CCTGCCAGCG CGCCGTGCAG GGCGTGGACC GGGTGCTGCA CCAGGCCGCG CTGGGTTCGG TGCCGCGTTC GCTGAAAGAC CCGATCACGA CCAACGAGGT CAACATCGGC GGTTTCCTGA ACATGCTGGT GGCCGCGCGC GATGCGCAGG TCCAGGCGTT CGTCTATGCG GCCTCGAGCT CGACCTACGG CGACCATCCC GACCTGCCCA AGGTGGAGGA GCGCATCGGC AATCCGCTCT CGCCGTACGC GGTGACCAAG TACGTCAACG AGCTGTACGC CGACGTGTTC GCGCGCTCGT ACGGGTTTTC CAGCGTGGGG CTGCGCTACT TCAACGTGTT CGGCAAGCGC CAGGACCCGG ACGGCGCGTA CGCGGCGGTG ATTCCGAAGT GGACGGCGGC GATGATCAAG GGCGAGGACG TCGTGATCAA CGGCGACGGC CAGACCAGCC GCGACTTCTG CTTCGTGGAG AACGCGGTGC AGGCCAACCT GCTGGCGGCG ATGGCCGCAC CGGAAGGCGC GAACCAGGTC TACAACGTGG CGTACAACGC GCGCACGACG CTGACCGAGC TGTTCGAGCA CCTGCGGCGC ACGCTGGCCG GGCAGGGCGT GTCGTACGAG AAGGCGCCGG TGTACGCGGA ATTCCGCGCC GGCGACGTGC GCCACTCGCA GGCCGACATC GGCAAGGCCG GCAAGCTGCT GGGCTACGAG CCGGCCTACG ACATCCTGCG CGGGCTGGAA GCCGCCATGC CCTGGTACAC GCAATTCCTG CGA

Details for BopeA.00085.a.B2.PW39367

PURIFICATION DATe:	1/10/2025
CONCENTRATION:	14.1mg/ml
OBSERVED MW:	38kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	7
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

MAHHHHHHTN RFETTCAQLR AQPQKWLVTG CAGFIGSNLL ETLLGLDQAV VGLDNFATGH QHNLDEVRAA VTPEQWARFT FIEGDIRDLA ACQRAVQGVD RVLHQAALGS VPRSLKDPIT TNEVNIGGFL NMLVAARDAQ VQAFVYAASS STYGDHPDLP KVEERIGNPL SPYAVTKYVN ELYADVFARS YGFSSVGLRY FNVFGKRQDP DGAYAAVIPK WTAAMIKGED VVINGDGQTS RDFCFVENAV QANLLAAMAA PEGANQVYNV AYNARTTLTE LFEHLRRTLA GQGVSYEKAP VYAEFRAGDV RHSQADIGKA GKLLGYEPAY DILRGLEAAM PWYTQFLR

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATACGAATC GCTTTGAGAC CACGTGCGCG CAGTTGCGGG CCCAACCCCA GAAATGGCTG GTGACCGGCT GCGCCGGCTT CATCGGCTCG AACCTGCTCG AGACGTTGCT CGGGCTGGAC CAGGCGGTGG TGGGGCTGGA CAACTTCGCC ACGGGACACC AGCACAACCT GGACGAGGTG CGCGCGGCGG TGACGCCCGA GCAATGGGCG CGCTTCACCT TCATCGAAGG CGACATCCGC GACCTGGCGG CCTGCCAGCG CGCCGTGCAG GGCGTGGACC GGGTGCTGCA CCAGGCCGCG CTGGGTTCGG TGCCGCGTTC GCTGAAAGAC CCGATCACGA CCAACGAGGT CAACATCGGC GGTTTCCTGA ACATGCTGGT GGCCGCGCGC GATGCGCAGG TCCAGGCGTT CGTCTATGCG GCCTCGAGCT CGACCTACGG CGACCATCCC GACCTGCCCA AGGTGGAGGA GCGCATCGGC AATCCGCTCT CGCCGTACGC GGTGACCAAG TACGTCAACG AGCTGTACGC CGACGTGTTC GCGCGCTCGT ACGGGTTTTC CAGCGTGGGG CTGCGCTACT TCAACGTGTT CGGCAAGCGC CAGGACCCGG ACGGCGCGTA CGCGGCGGTG ATTCCGAAGT GGACGGCGGC GATGATCAAG GGCGAGGACG TCGTGATCAA CGGCGACGGC CAGACCAGCC GCGACTTCTG CTTCGTGGAG AACGCGGTGC AGGCCAACCT GCTGGCGGCG ATGGCCGCAC CGGAAGGCGC GAACCAGGTC TACAACGTGG CGTACAACGC GCGCACGACG CTGACCGAGC TGTTCGAGCA CCTGCGGCGC ACGCTGGCCG GGCAGGGCGT GTCGTACGAG AAGGCGCCGG TGTACGCGGA ATTCCGCGCC GGCGACGTGC GCCACTCGCA GGCCGACATC GGCAAGGCCG GCAAGCTGCT GGGCTACGAG CCGGCCTACG ACATCCTGCG CGGGCTGGAA GCCGCCATGC CCTGGTACAC GCAATTCCTG CGATGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT