BobuA.20387.a

ErpQ protein (plasmid) (BB_N39)

CENTER ID: BobuA.20387.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.20387.a.B1.GE43869 full length 1 343
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.301
UniProt: Q9S035

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNKKTLIICA VFALIISCKN FATGKDIKQN SEGKIKGFVN KILDPVKDKI ASSGTKVDEV AKKLQEEEKE ELMQGDDPNG SGINPPPVLP ENIHNNALVL KAIEQSDGQQ EKKVEEAEAK VEENKEKQEN TEENIKEKEI IDEQNKQELA KAKEEEQQKE QKRHQEEQQR KAKAEKEKRE REEAEQQKRQ QEEEEKRQVD NQIKTLIAKI DEINENIDVI KWQTTVGPQG VIDRITGPVY DDFTNGNNSI RETWEGLEEE SEDEGLGKLL KELSDARDAL RTKLNEGNKP YTGYEEPKLK ESVNVSEIKE DLEKLKSKLE EVKKYLKDSS KFEEIKGYIS DSQ
NT Sequence
atgaataaaa aaacattgat tatttgtgct gtttttgcgc tgataatttc ttgcaagaat tttgcaactg gtaaagatat aaaacaaaat tcagaaggga aaattaaagg atttgtaaat aagattttag atccagtaaa ggataaaatt gcttcaagtg gtacaaaagt agatgaagta gcaaaaaaat tacaagaaga agaaaaagaa gaattaatgc agggcgatga tcctaatggc agtggaataa atccgccacc agtattgccg gaaaatattc acaataatgc attagtatta aaagcaatag aacaaagtga tggtcaacaa gaaaaaaaag tagaagaagc tgaagctaaa gttgaagaaa ataaagaaaa acaagagaat acagaagaaa acattaaaga aaaagaaata atagacgaac aaaacaaaca agaattagct aaagctaaag aagaagaaca acaaaaagaa caaaaaagac atcaagaaga gcaacaaaga aaagctaaag cagaaaaaga aaaaagagaa agagaagagg cagaacaaca aaaacgacaa caagaagagg aagaaaaaag gcaagttgat aaccaaatta aaacacttat agctaaaata gatgagatca atgaaaatat tgatgttata aaatggcaaa cgactgtagg cccacaaggc gttatagata gaattactgg gcctgtgtat gatgatttta ccaatggcaa taattctata cgcgaaactt gggaggggtt agaagaggaa tcagaagacg aaggattagg aaaattattg aaagaattga gtgatgctag ggacgcgcta agaactaaat taaatgaagg caataaacca tatactggtt acgaagagcc taagttaaaa gaaagtgtaa atgttagcga aattaaagaa gatttagaaa aattaaaatc aaaattagaa gaagttaaaa aatatcttaa agatagttct aaatttgaag aaattaaagg atacatcagt gacagtcagt aa
Details for BobuA.20387.a.B1.GE43869
HARVESTED ON: data unavailable
SEQUENCED ON: 12/2/2020
EXPECTED MW: 40kDa
OBSERVED MW: 22kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 96
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMN KKTLIICAVF ALIISCKNFA TGKDIKQNSE GKIKGFVNKI LDPVKDKIAS SGTKVDEVAK KLQEEEKEEL MQGDDPNGSG INPPPVLPEN IHNNALVLKA IEQSDGQQEK KVEEAEAKVE ENKEKQESTE ENIKEKEIID EQNKQELAKA KEEEQQKEQK RHQEEQQRKA KAEKEKRERE EAEQQKRQQE EEEKRQVDNQ IKTLIAKIDE INENIDVIKW QTTVGPQGVI DRITGPVYDD FTNGNNSIRE TWEGLEEESE DEGLGKLLKE LSDARDALRT KLNESNKPYT GYEEPKLKES VNVSEIKEDL EKLKSNLELS LRYFLTSSNF EEIKGYISDS Q
Validated NT Sequence
atggctcacc accaccacca ccatatgaat aaaaaaacat tgattatttg tgctgttttt gcgctgataa tttcttgcaa gaattttgca actggtaaag atataaaaca aaattcagaa gggaaaatta aaggatttgt aaataagatt ttagatccag taaaggataa aattgcttca agtggtacaa aagtagatga agtagcaaaa aaattacaag aagaagaaaa agaagaatta atgcagggcg atgatcctaa tggcagtgga ataaatccgc caccagtatt gccggaaaat attcacaata atgcattagt attaaaagca atagaacaaa gtgatggtca acaagaaaaa aaagtagaag aagctgaagc taaagttgaa gaaaataaag aaaaacaaga gagtacagaa gaaaacatta aagaaaaaga aataatagac gaacaaaaca aacaagaatt agctaaagct aaagaagaag aacaacaaaa agaacaaaaa agacatcaag aagagcaaca aagaaaagct aaagcagaaa aagaaaaaag agaaagagaa gaggcagaac aacaaaaacg acaacaagaa gaggaagaaa aaaggcaagt tgataaccaa attaaaacac ttatagctaa aatagatgag atcaatgaaa atattgatgt tataaaatgg caaacgactg taggcccaca aggcgttata gatagaatta ctgggcctgt gtatgatgat tttaccaatg gcaataattc tatacgcgaa acttgggagg ggttagaaga ggaatcagaa gacgaaggat taggaaaatt attgaaagaa ttgagtgatg ctagggacgc gctaagaact aaattaaatg aaagcaataa accatatact ggttacgaag agcctaagtt aaaagaaagt gtaaatgtta gcgaaattaa agaagattta gaaaaattaa aatcaaattt agaactatct ttaagatatt ttttaacttc ttctaatttt gaagaaatta aaggatacat cagtgacagt cagtaagtga gtaagat
Expected Protein Sequence
MAHHHHHHMN KKTLIICAVF ALIISCKNFA TGKDIKQNSE GKIKGFVNKI LDPVKDKIAS SGTKVDEVAK KLQEEEKEEL MQGDDPNGSG INPPPVLPEN IHNNALVLKA IEQSDGQQEK KVEEAEAKVE ENKEKQENTE ENIKEKEIID EQNKQELAKA KEEEQQKEQK RHQEEQQRKA KAEKEKRERE EAEQQKRQQE EEEKRQVDNQ IKTLIAKIDE INENIDVIKW QTTVGPQGVI DRITGPVYDD FTNGNNSIRE TWEGLEEESE DEGLGKLLKE LSDARDALRT KLNEGNKPYT GYEEPKLKES VNVSEIKEDL EKLKSKLEEV KKYLKDSSKF EEIKGYISDS Q
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaata aaaaaacatt gattatttgt gctgtttttg cgctgataat ttcttgcaag aattttgcaa ctggtaaaga tataaaacaa aattcagaag ggaaaattaa aggatttgta aataagattt tagatccagt aaaggataaa attgcttcaa gtggtacaaa agtagatgaa gtagcaaaaa aattacaaga agaagaaaaa gaagaattaa tgcagggcga tgatcctaat ggcagtggaa taaatccgcc accagtattg ccggaaaata ttcacaataa tgcattagta ttaaaagcaa tagaacaaag tgatggtcaa caagaaaaaa aagtagaaga agctgaagct aaagttgaag aaaataaaga aaaacaagag aatacagaag aaaacattaa agaaaaagaa ataatagacg aacaaaacaa acaagaatta gctaaagcta aagaagaaga acaacaaaaa gaacaaaaaa gacatcaaga agagcaacaa agaaaagcta aagcagaaaa agaaaaaaga gaaagagaag aggcagaaca acaaaaacga caacaagaag aggaagaaaa aaggcaagtt gataaccaaa ttaaaacact tatagctaaa atagatgaga tcaatgaaaa tattgatgtt ataaaatggc aaacgactgt aggcccacaa ggcgttatag atagaattac tgggcctgtg tatgatgatt ttaccaatgg caataattct atacgcgaaa cttgggaggg gttagaagag gaatcagaag acgaaggatt aggaaaatta ttgaaagaat tgagtgatgc tagggacgcg ctaagaacta aattaaatga aggcaataaa ccatatactg gttacgaaga gcctaagtta aaagaaagtg taaatgttag cgaaattaaa gaagatttag aaaaattaaa atcaaaatta gaagaagtta aaaaatatct taaagatagt tctaaatttg aagaaattaa aggatacatc agtgacagtc agtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat