SSGCID: Target Page

BobuA.19134.a

Uncharacterized protein (BB_0325)

CENTER ID:	BobuA.19134.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.19134.a.B2.GE39595	mature protein		16	369

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.19134.a.B2.PW37850	mature protein		16	369

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224326.49.peg.722
RefSeq:	NP_212459.1
UniProt:	O51304

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKKIFLFLFI SFYLFGFEDS SLKIGIDDVY VEAHEEGFHL FIRKKPAIKS VILTESFEIP DKKKDVATYS FRTLSYNKVN GDEIRILNGR VIKNKELLSL TSSTPVPNKK FGEAFHILIP KKLKYGFPNF STRSGDIDLE VLKSKKEPFW FSIRSFEKKY NDYLGRYQDN AYELLFKDDQ NQGKIEFNEL KDTFTKFSDE VVIANNGIDI VDKINKILKN SEDSVYDLDL VLVVDVTDSM KSNIEILKEH LFSIIEPQLQ KFKSYRIGLV FYKDYLEDFL TKAFDFNTIP YLNNILKYVN VGGGGDYPEA VFEGIDAAVT QFDWRAERRF IIVIGDAPPH EYPRGSIVYK DVINSAKEKD ITIYGIIFQ

NT Sequence

atgaagaaaa tttttttatt tctttttatt agtttttatt tgtttggatt tgaagatagt tctttgaaaa taggtattga tgatgtttat gttgaggctc atgaagaggg atttcatctt tttattagaa aaaaacctgc aatcaaatca gtaatattga cagagtcttt tgaaattcct gataagaaaa aagatgtggc tacttattca tttcgtacat taagttataa taaggttaat ggagatgaaa ttcggatttt aaatggaaga gttattaaga ataaagaact tttatcattg acatcttcca cccctgttcc taataaaaag tttggagaag cttttcatat attgattcca aaaaaattaa aatatggatt tccaaatttt tcaacaagaa gtggtgatat tgacttagaa gtattaaaaa gtaaaaaaga gcccttttgg ttttctataa gatcttttga gaaaaaatat aatgattatt tgggcagata tcaagacaat gcttatgaat tgcttttcaa ggatgatcaa aatcagggaa aaattgaatt taatgaatta aaagatactt ttacaaaatt ttcagatgag gttgttattg ctaataatgg cattgatatt gttgataaaa taaacaaaat tttaaaaaac tcagaagatt cagtttatga tttagattta gtgcttgttg ttgatgttac tgatagtatg aaaagcaata ttgagattct aaaagagcat ttgttttcaa taatagaacc tcaacttcaa aagtttaaat cctacagaat aggtcttgtt ttttataaag actatcttga agatttttta accaaagctt ttgattttaa tactattcct tatttaaata atattcttaa gtatgttaat gttggtggcg gtggggatta tccagaagct gtttttgagg ggattgatgc tgctgtgacc caatttgatt ggcgggcaga aagaaggttt attattgtta taggagatgc acctcctcat gagtatccaa gagggtctat tgtttataaa gatgttatca attctgcaaa ggaaaaagat attacaattt atggaataat atttcagtaa

Details for BobuA.19134.a.B2.GE39595

HARVESTED ON:	10/30/2015
SEQUENCED ON:	10/23/2015
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMG FEDSSLKIGI DDVYVEAHEE GFHLFIRKKP AIKSVILTES FEIPDKKKDV ATYSFRTLSY NKVNGDEIRI LNGRVIKNKE LLSLTSSTPV PNKKFGEAFH ILIPKKLKYG FPNFSTRSGD IDLEVLKSKK EPFWFSIRSF EKKYNDYLGR YQDNAYELLF KDDQNQGKIE FNELKDTFTK FSDEVVIANN GIDIVDKINK ILKNSEDSVY DLDLVLVVDV TDSMKSNIEI LKEHLFSIIE PQLQKFKSYR IGLVFYKDYL EDFLTKAFDF NTIPYLNNIL KYVNVGGGGD YPEAVFEGID AAVTQFDWRA ERRFIIVIGD APPHEYPRGS IVYKDVINSA KEK

Validated NT Sequence

ccatggctca ccaccaccac caccatatgg gatttgaaga tagttctttg aaaataggta ttgatgatgt ttatgttgag gctcatgaag agggatttca tctttttatt agaaaaaaac ctgcaatcaa atcagtaata ttgacagagt cttttgaaat tcctgataag aaaaaagatg tggctactta ttcatttcgt acattaagtt ataataaggt taatggagat gaaattcgga ttttaaatgg aagagttatt aagaataaag aacttttatc attgacatct tccacccctg ttcctaataa aaagtttgga gaagcttttc atatattgat tccaaaaaaa ttaaaatatg gatttccaaa tttttcaaca agaagtggtg atattgactt agaagtatta aaaagtaaaa aagagccctt ttggttttct ataagatctt ttgagaaaaa atataatgat tatttgggca gatatcaaga caatgcttat gaattgcttt tcaaggatga tcaaaatcag ggaaaaattg aatttaatga attaaaagat acttttacaa aattttcaga tgaggttgtt attgctaata atggcattga tattgttgat aaaataaaca aaattttaaa aaactcagaa gattcagttt atgatttaga tttagtgctt gttgttgatg ttactgatag tatgaaaagc aatattgaga ttctaaaaga gcatttgttt tcaataatag aacctcaact tcaaaagttt aaatcctaca gaataggtct tgttttttat aaagactatc ttgaagattt tttaaccaaa gcctttgatt ttaatactat tccttattta aataatattc ttaagtatgt taatgttggt ggcggtgggg attatccaga agctgttttt gaggggattg atgctgctgt gacccaattt gattggcggg cagaaagaag gtttattatt gttataggag atgcacctcc tcatgagtat ccaagagggt ctattgttta taaagatgtt atcaattctg caaaggaaaa aga

Expected Protein Sequence

MAHHHHHHGF EDSSLKIGID DVYVEAHEEG FHLFIRKKPA IKSVILTESF EIPDKKKDVA TYSFRTLSYN KVNGDEIRIL NGRVIKNKEL LSLTSSTPVP NKKFGEAFHI LIPKKLKYGF PNFSTRSGDI DLEVLKSKKE PFWFSIRSFE KKYNDYLGRY QDNAYELLFK DDQNQGKIEF NELKDTFTKF SDEVVIANNG IDIVDKINKI LKNSEDSVYD LDLVLVVDVT DSMKSNIEIL KEHLFSIIEP QLQKFKSYRI GLVFYKDYLE DFLTKAFDFN TIPYLNNILK YVNVGGGGDY PEAVFEGIDA AVTQFDWRAE RRFIIVIGDA PPHEYPRGSI VYKDVINSAK EKDITIYGII FQ

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggatttg aagatagttc tttgaaaata ggtattgatg atgtttatgt tgaggctcat gaagagggat ttcatctttt tattagaaaa aaacctgcaa tcaaatcagt aatattgaca gagtcttttg aaattcctga taagaaaaaa gatgtggcta cttattcatt tcgtacatta agttataata aggttaatgg agatgaaatt cggattttaa atggaagagt tattaagaat aaagaacttt tatcattgac atcttccacc cctgttccta ataaaaagtt tggagaagct tttcatatat tgattccaaa aaaattaaaa tatggatttc caaatttttc aacaagaagt ggtgatattg acttagaagt attaaaaagt aaaaaagagc ccttttggtt ttctataaga tcttttgaga aaaaatataa tgattatttg ggcagatatc aagacaatgc ttatgaattg cttttcaagg atgatcaaaa tcagggaaaa attgaattta atgaattaaa agatactttt acaaaatttt cagatgaggt tgttattgct aataatggca ttgatattgt tgataaaata aacaaaattt taaaaaactc agaagattca gtttatgatt tagatttagt gcttgttgtt gatgttactg atagtatgaa aagcaatatt gagattctaa aagagcattt gttttcaata atagaacctc aacttcaaaa gtttaaatcc tacagaatag gtcttgtttt ttataaagac tatcttgaag attttttaac caaagctttt gattttaata ctattcctta tttaaataat attcttaagt atgttaatgt tggtggcggt ggggattatc cagaagctgt ttttgagggg attgatgctg ctgtgaccca atttgattgg cgggcagaaa gaaggtttat tattgttata ggagatgcac ctcctcatga gtatccaaga gggtctattg tttataaaga tgttatcaat tctgcaaagg aaaaagatat tacaatttat ggaataatat ttcagtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for BobuA.19134.a.B2.PW37850

PURIFICATION DATe:	2/2/2016
CONCENTRATION:	26.5mg/ml
OBSERVED MW:	42kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	28
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGGATTTG AAGATAGTTC TTTGAAAATA GGTATTGATG ATGTTTATGT TGAGGCTCAT GAAGAGGGAT TTCATCTTTT TATTAGAAAA AAACCTGCAA TCAAATCAGT AATATTGACA GAGTCTTTTG AAATTCCTGA TAAGAAAAAA GATGTGGCTA CTTATTCATT TCGTACATTA AGTTATAATA AGGTTAATGG AGATGAAATT CGGATTTTAA ATGGAAGAGT TATTAAGAAT AAAGAACTTT TATCATTGAC ATCTTCCACC CCTGTTCCTA ATAAAAAGTT TGGAGAAGCT TTTCATATAT TGATTCCAAA AAAATTAAAA TATGGATTTC CAAATTTTTC AACAAGAAGT GGTGATATTG ACTTAGAAGT ATTAAAAAGT AAAAAAGAGC CCTTTTGGTT TTCTATAAGA TCTTTTGAGA AAAAATATAA TGATTATTTG GGCAGATATC AAGACAATGC TTATGAATTG CTTTTCAAGG ATGATCAAAA TCAGGGAAAA ATTGAATTTA ATGAATTAAA AGATACTTTT ACAAAATTTT CAGATGAGGT TGTTATTGCT AATAATGGCA TTGATATTGT TGATAAAATA AACAAAATTT TAAAAAACTC AGAAGATTCA GTTTATGATT TAGATTTAGT GCTTGTTGTT GATGTTACTG ATAGTATGAA AAGCAATATT GAGATTCTAA AAGAGCATTT GTTTTCAATA ATAGAACCTC AACTTCAAAA GTTTAAATCC TACAGAATAG GTCTTGTTTT TTATAAAGAC TATCTTGAAG ATTTTTTAAC CAAAGCTTTT GATTTTAATA CTATTCCTTA TTTAAATAAT ATTCTTAAGT ATGTTAATGT TGGTGGCGGT GGGGATTATC CAGAAGCTGT TTTTGAGGGG ATTGATGCTG CTGTGACCCA ATTTGATTGG CGGGCAGAAA GAAGGTTTAT TATTGTTATA GGAGATGCAC CTCCTCATGA GTATCCAAGA GGGTCTATTG TTTATAAAGA TGTTATCAAT TCTGCAAAGG AAAAAGATAT TACAATTTAT GGAATAATAT TTCAGTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT