BobuA.19127.a

carboxyl-terminal protease (ctp) (BB_0359)

CENTER ID: BobuA.19127.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.19127.a.B2.GE39588 mature protein 28 475
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKNKFLICVY FLLTLGISSL VIVESIFAFD ESNNKLSRSN YEQMMIQAFE FVKENYVDPV SDEVIFEGAL KGIFQSLGDP YSQYLTKKDL EEISKTTVGD YVGIGISIIK KMHSQDKQDK AKDFDPNSAC VSIVTPFEGG PAYKAGIKSG DCITAVDGKS VYSMEVDQVV DLLKGKEGTK VKVSILRGKN LTLDFELTRE KIEIQTIKYD VINSDIGYIR IVSFNPHTSV DFRKALDNLK NKNIKSLILD LRLNTGGYFQ AAIKMADDIL SKGIIVSTKS RNSSKPIDYK ASSKQVLPSN IKIVALIDRS SASASEVFVG ALKDNKRAYI IGEKSYGKGL IQHVVPFYTG GFKITSSKYY TPSGKSIHKV GIEPDLEIKS PDFSEEEALI YKEIFDKKLI EGFLKGKKFI TEQEIDFFVE NLVKENPKYK IDKEFLGKYV FFNYYQDNNK ELPIYNLHYD KVLKTACEYL SKLGN
NT Sequence
atgaaaaata aatttttaat atgtgtatat tttttattga ctctgggtat aagctcttta gtaattgttg aatctatttt tgcttttgat gaatctaata ataagttatc aagatcaaat tatgagcaga tgatgattca agcttttgaa tttgtaaaag aaaattatgt tgatcctgta agtgatgaag taatttttga aggtgcttta aaaggaatat ttcaatccct aggcgatcct tattctcaat atttgacaaa aaaagattta gaagaaattt caaaaacaac agtaggagat tatgttggca ttggaatttc tataataaaa aaaatgcatt cccaagataa gcaagacaag gcaaaagatt ttgatcctaa tagtgcttgt gtttctattg ttacgccttt tgaaggaggt ccggcttata aggctggaat taaatctgga gattgtatta ccgctgttga tggcaagagt gtttattcta tggaagtaga tcaagttgtt gatcttttaa aaggtaaaga aggcacaaaa gttaaagtat ctattcttag gggaaaaaat ttaacattgg attttgaact tacaagagag aagatagaaa tacaaacrat caagtatgac gttattaatt cagatattgg ctatataaga atagtaagct ttaatccaca cacctctgta gattttagaa aagctttaga taatcttaag aataaaaata ttaaatcttt aattttagat ttaaggctta ataccggagg atattttcag gcagctataa aaatggcgga tgatatttta tctaaaggaa ttattgtttc cacaaaatca agaaattcta gcaagcctat tgattataag gcaagctcaa aacaagtttt gccttcaaat ataaaaattg ttgctttaat agacagatca tcagcctcag catcagaggt ttttgtagga gccttaaaag acaataagag agcatacatt ataggggaaa agtcttatgg caaggggctt attcagcatg tagttccttt ttatactggt ggatttaaaa ttacaagctc aaagtattat actccatctg gaaagagtat tcataaggtt ggaattgagc ctgatttgga aataaaatct ccagattttt ctgaggagga ggcattaata tataaagaaa tttttgataa aaagctgata gaaggttttt tgaagggtaa aaaattcatt accgaacaag agattgattt ttttgttgag aatcttgtaa aagaaaatcc aaaatataaa attgataaag aatttttagg caagtatgtg ttttttaatt actatcagga caataataaa gaattgccaa tttataatct acattatgac aaagttttaa aaacagcttg tgagtatttg tctaaattag gtaattaa
Details for BobuA.19127.a.B2.GE39588
HARVESTED ON: data unavailable
SEQUENCED ON: 10/23/2015
EXPECTED MW: 51kDa
OBSERVED MW: 0kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: None
TOTAL EXPRESSION LEVEL: Data Unavailable
SOLUBLE EXPRESSION LEVEL Data Unavailable
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 34
PERCENT COVERAGE: 23
Validated AA Sequence
MAHHHHHHMA FDESNNKLSR SNYEQMMIQA FEXXXKLXXX XXXXXXFXXX XXXXXXXXXX XLFXXXXXKX XXKNXXNNXX RXXXXXXFYN KKNXXXXXXR QXXXX
Validated NT Sequence
atggctcacc accaccacca ccatatggct tttgatgaat ctaataataa gttatcaaga tcaaattatg agcagatgat gattcaagct tttgaattnn nangaaaatt atgnnnnnnn nntnnnnnnn nnnnnnattt ttgnnnnnnn nnnnnnannn nnnnnnnnnn nncnnnnnga nnnttattct nnnnannngn nnaaaaaann nnnnnaaaaa attnnnnnaa caacnnnnnn agannntgnn ngnnttnnnn tttctataat aaaaaaaatg nnttnnnnnn atannnaaga caagnnnnan nnnnntgann nnantnnnnn nngtgnnnct attgtnnnnn cttttgannn agnnnngnnn nntannnnnn nnnttnnntc tgnnnannnt gttaccgctg nnnnnnncnn nnantgntta ttctatgnnn nnnnnatcaa gttgttgann nttnnnnang taaagnnnnn acnnnnntta aagtatctat tctnngggga aaaaatttaa cattnnnntt tgaacttaca agagagaaga tagaaataca aacaatcaag tatgacgtta ttaattcaga tattggctat ataagaatag taagctttaa tccacacacc tctgtagatt ttagaaaagc tttagataat cttaagaata aaaatattaa atctttaatt ttagatttaa ggcttaatac cggaggatat tttcaggcag ctataaaaat ggcggatgat attttatcta aaggaattat tgtttccaca aaatcaagaa attctagcaa gcctattgat tataaggcaa gctcaaaaca agttttgcct tcaaatataa aaattgttgc tttaatagac agatcatcag cctcagcatc agaggttttt gtaggagcct taaaagacaa taagagagca tacattatag gggaaaagtc ttatggcaag gggcttattc agcatgtagt tcctttttat actggtggat ttaaaattac aagctcaaag tattatactc catctggaaa gagtattcat aaggttggaa ttgagcctga tttggaaata aaatctccag atttttctga ggaggaggca ttaatatata aagaaatttt tgataaaaag ctgatagaag gttttttgaa gggtaaaaaa ttcattaccg aacaagagat tgattttttt gttgagaatc ttgtaaaaga aaatccaaaa tataaaattg ataaagaatt tttaggcaag tatgtgtttt ttaattacta tcaggacaat aataaagaat tgccaattta taatctacat tatgacaaag ttttaaaaa
Expected Protein Sequence
MAHHHHHHAF DESNNKLSRS NYEQMMIQAF EFVKENYVDP VSDEVIFEGA LKGIFQSLGD PYSQYLTKKD LEEISKTTVG DYVGIGISII KKMHSQDKQD KAKDFDPNSA CVSIVTPFEG GPAYKAGIKS GDCITAVDGK SVYSMEVDQV VDLLKGKEGT KVKVSILRGK NLTLDFELTR EKIEIQTIKY DVINSDIGYI RIVSFNPHTS VDFRKALDNL KNKNIKSLIL DLRLNTGGYF QAAIKMADDI LSKGIIVSTK SRNSSKPIDY KASSKQVLPS NIKIVALIDR SSASASEVFV GALKDNKRAY IIGEKSYGKG LIQHVVPFYT GGFKITSSKY YTPSGKSIHK VGIEPDLEIK SPDFSEEEAL IYKEIFDKKL IEGFLKGKKF ITEQEIDFFV ENLVKENPKY KIDKEFLGKY VFFNYYQDNN KELPIYNLHY DKVLKTACEY LSKLGN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcttttg atgaatctaa taataagtta tcaagatcaa attatgagca gatgatgatt caagcttttg aatttgtaaa agaaaattat gttgatcctg taagtgatga agtaattttt gaaggtgctt taaaaggaat atttcaatcc ctaggcgatc cttattctca atatttgaca aaaaaagatt tagaagaaat ttcaaaaaca acagtaggag attatgttgg cattggaatt tctataataa aaaaaatgca ttcccaagat aagcaagaca aggcaaaaga ttttgatcct aatagtgctt gtgtttctat tgttacgcct tttgaaggag gtccggctta taaggctgga attaaatctg gagattgtat taccgctgtt gatggcaaga gtgtttattc tatggaagta gatcaagttg ttgatctttt aaaaggtaaa gaaggcacaa aagttaaagt atctattctt aggggaaaaa atttaacatt ggattttgaa cttacaagag agaagataga aatacaaacr atcaagtatg acgttattaa ttcagatatt ggctatataa gaatagtaag ctttaatcca cacacctctg tagattttag aaaagcttta gataatctta agaataaaaa tattaaatct ttaattttag atttaaggct taataccgga ggatattttc aggcagctat aaaaatggcg gatgatattt tatctaaagg aattattgtt tccacaaaat caagaaattc tagcaagcct attgattata aggcaagctc aaaacaagtt ttgccttcaa atataaaaat tgttgcttta atagacagat catcagcctc agcatcagag gtttttgtag gagccttaaa agacaataag agagcataca ttatagggga aaagtcttat ggcaaggggc ttattcagca tgtagttcct ttttatactg gtggatttaa aattacaagc tcaaagtatt atactccatc tggaaagagt attcataagg ttggaattga gcctgatttg gaaataaaat ctccagattt ttctgaggag gaggcattaa tatataaaga aatttttgat aaaaagctga tagaaggttt tttgaagggt aaaaaattca ttaccgaaca agagattgat ttttttgttg agaatcttgt aaaagaaaat ccaaaatata aaattgataa agaattttta ggcaagtatg tgttttttaa ttactatcag gacaataata aagaattgcc aatttataat ctacattatg acaaagtttt aaaaacagct tgtgagtatt tgtctaaatt aggtaattga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at