BobuA.19121.a

Uncharacterized protein (BB_0405)

CENTER ID: BobuA.19121.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.19121.a.B2.GE39584 mature protein 18 203
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.799
RefSeq: NP_212539.1
UniProt: O51366

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRMLLATIIL ILTTGLLAAQ SKSKSMTEDD FDFDKLLAKE ESVRRLFGIG FGVGYPLANI TISVPYVDID LGYGGFVGLK PNNFLPYVVM GVDLLFKDEI HKNTMISGGI GIGADWSKGS PEKSNEKLEE EEENEAQQVA SLQNRIGVVI RLPLVIEYSF LKNIVIGFKA VATIGTTMLL GSPMSFEGAR FNFLGTGFIK IYI
NT Sequence
atgagaatgc tattagcaac aataatactt atattaacaa cgggtttatt agctgcacaa tccaaaagca aaagtatgac tgaagatgac tttgattttg ataaacttct tgcaaaagaa gagtctgtgc gccgtttatt tggcataggt tttggagttg gatatccact tgcaaacatt acaatatctg ttccatatgt agacatagac cttgggtacg gaggattcgt agggcttaaa cccaacaatt tcttgcccta tgttgtgatg ggtgtagatc ttctatttaa agatgaaata cacaaaaaca ctatgatttc tggaggcatt ggaataggtg cagattggtc aaaaggaagt cctgaaaaat caaatgaaaa acttgaagaa gaggaagaaa atgaagcaca acaagtagct tctcttcaaa atagaatagg ggttgtgata agattgcctt tggtaataga gtacagcttt cttaaaaata ttgtgattgg atttaaagct gttgctacta ttggaacaac tatgctactt ggcagcccaa tgtcatttga aggagctaga tttaatttct taggcacagg ctttataaaa atatatatat ag
Details for BobuA.19121.a.B2.GE39584
HARVESTED ON: 10/30/2015
SEQUENCED ON: 10/23/2015
EXPECTED MW: 21kDa
OBSERVED MW: 24kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA AQSKSKSMTE DDFDFDKLLA KEESVRRLFG IGFGVGYPLA NITISVPYVD IDLGYGGFVG LKPNNFLPYV VMGVDLLFKD EIHKNTMISG GIGIGADWSK GSPGKSNEKL EEEEENEAQQ VASLQNRIGV VIRLPLVIEY SFLKNIVIGF KAVATIGTTM LLGSPMSFEG ARFNFLGTGF IKIYI
Validated NT Sequence
atggctcacc accaccacca ccatatggct gcacaatcca aaagcaaaag tatgactgaa gatgactttg attttgataa acttcttgca aaagaagagt ctgtgcgccg tttatttggc ataggttttg gagttggata tccacttgca aacattacaa tatctgttcc atatgtagac atagaccttg ggtacggagg attcgtaggg cttaaaccca acaatttctt gccctatgtt gtgatgggtg tagatcttct atttaaagat gaaatacaca aaaacactat gatttctgga ggcattggaa taggtgcaga ttggtcaaaa ggaagtcctg gaaaatcaaa tgaaaaactt gaagaagagg aagaaaatga agcacaacaa gtagcttctc ttcaaaatag aataggggtt gttataagat tgcctttggt aatagagtac agctttctta aaaatattgt gattggattt aaagctgttg ctactattgg aacaactatg ctacttggca gcccaatgtc atttgaagga gctagattta atttcttagg cacaggcttt ataaaaatat atatataagt gagtaagat
Expected Protein Sequence
MAHHHHHHAA QSKSKSMTED DFDFDKLLAK EESVRRLFGI GFGVGYPLAN ITISVPYVDI DLGYGGFVGL KPNNFLPYVV MGVDLLFKDE IHKNTMISGG IGIGADWSKG SPEKSNEKLE EEEENEAQQV ASLQNRIGVV IRLPLVIEYS FLKNIVIGFK AVATIGTTML LGSPMSFEGA RFNFLGTGFI KIYI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgctgcac aatccaaaag caaaagtatg actgaagatg actttgattt tgataaactt cttgcaaaag aagagtctgt gcgccgttta tttggcatag gttttggagt tggatatcca cttgcaaaca ttacaatatc tgttccatat gtagacatag accttgggta cggaggattc gtagggctta aacccaacaa tttcttgccc tatgttgtga tgggtgtaga tcttctattt aaagatgaaa tacacaaaaa cactatgatt tctggaggca ttggaatagg tgcagattgg tcaaaaggaa gtcctgaaaa atcaaatgaa aaacttgaag aagaggaaga aaatgaagca caacaagtag cttctcttca aaatagaata ggggttgtga taagattgcc tttggtaata gagtacagct ttcttaaaaa tattgtgatt ggatttaaag ctgttgctac tattggaaca actatgctac ttggcagccc aatgtcattt gaaggagcta gatttaattt cttaggcaca ggctttataa aaatatatat atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat