SSGCID: Target Page

BobuA.19111.a

Uncharacterized protein (BB_0043)

CENTER ID:	BobuA.19111.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.19111.a.B2.GE39580	mature protein		20	346

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224326.49.peg.441
RefSeq:	NP_212177.1
RefSeq:	WP_010889664.1
UniProt:	O51072

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIRKYLIYIS LLFIVFEVYS KPAFISQDDS YELDFSSGEV DISVNTNSKF NLSFKDESWI YIKSIENEAF IKLIGESYDN GAVFTFQTFK KEGKIKLVFT YQNVKDSSEF NKIIILKITK NFEVAIPQGV GGGSSRDNNI ETGNNLELGG GSISGATSKE IIVRALNLSY INDYKGAIDL LNKYNFNDDK YILLKAEIHY KNGDYLKSYE NYLKLKSKYF QSIVFDLIRL AIELNIKEEV LENARYLVEK NVDFSESIYL EIFEFLVTRG EHEFALNFSS LYFPKYINSS FSDKYSYLLG KLYESESKHK DFLKALHYYK LVIDNYPFSY YYERAKIRYL FLKRFF

NT Sequence

atgattagaa aatatttgat ttatataagt ttgctattta ttgtttttga agtttactct aagccagctt ttataagtca agacgattcg tatgagcttg attttagtag tggagaggta gatattagtg taaataccaa ttcaaaattt aatctttctt ttaaagatga gtcttggatt tatatcaaaa gcattgaaaa tgaagctttt attaagttaa ttggagaatc ttatgataac ggtgctgttt ttacttttca gacttttaaa aaagaaggca aaattaaatt ggttttcact tatcaaaatg ttaaagattc aagtgaattt aataaaataa ttatcttgaa aattacaaag aattttgaag ttgcaattcc acaaggcgtt ggtggtggct ctagcaggga caataacatt gaaactggta ataatcttga acttgggggg gggagtatta gcggggcaac ttctaaagag attattgtta gggctttaaa tttgtcctac ataaatgatt acaaaggagc aatagatttg cttaataagt ataatttcaa tgacgataaa tatattttat tgaaggcgga aattcattat aaaaatggtg attatttaaa atcttatgaa aattatttga aattgaagag taaatatttt caaagcattg tttttgatct aattaggctt gctatagaat taaatattaa agaagaggtt ttagagaacg ctagatattt agttgaaaag aatgttgatt tttctgagag catttatctt gagatctttg aattcttagt aacaagggga gagcatgagt ttgctttaaa ttttagctct ctttactttc ctaagtatat taattcaagc ttttcagaca aatatagtta tttgttggga aaactttatg agtctgagag caagcataaa gattttttaa aggctttgca ttactataaa ttggttattg ataattaccc ttttagttat tattatgaga gagccaagat aagatattta tttttaaagc ggttttttta g

Details for BobuA.19111.a.B2.GE39580

HARVESTED ON:	10/30/2015
SEQUENCED ON:	10/23/2015
EXPECTED MW:	39kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHSK PAFISQDDSY ELDFSSGEVD ISVNTNSKFN LSFKDESWIY IKSIENEAFI KLIGESYDNG AVFTFQTFKK EGKIKLVFTY QNVKDSSEFN KIIILKITKN FEVAIPQGVG GGSSRDNNIE TGNNLELGGG SISGATSKEI IVRALNLSYI NDYKGAIDLL NKYNFNDDKY ILLKAEIHYK NGDYLKSYEN YLKLKSKYFQ SIVFDLIRLA IELNIKEEVL ENARYLVEKN VDFSESIYLE IFEFLVTRGE HEFALNFSSL YFPKYINSSF SDKYSYLLGK LYESESKHKD FLKALHYYKL VIDNYPFSYY YERAKIRYLF LKRFF

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattctaagc cagcttttat aagtcaagac gattcgtatg agcttgattt tagtagtgga gaggtagata ttagtgtaaa taccaattca aaatttaatc tttcttttaa agatgagtct tggatttata tcaaaagcat tgaaaatgaa gcttttatta agttaattgg agaatcttat gataacggtg ctgtttttac ttttcagact tttaaaaaag aaggcaaaat taaattggtt ttcacttatc aaaatgttaa agattcaagt gaatttaata aaataattat cttgaaaatt acaaagaatt ttgaagttgc aattccacaa ggcgttggtg gtggctctag cagggacaat aacattgaaa ctggtaataa tcttgaactt ggggggggga gtattagcgg ggcaacttct aaagagatta ttgttagggc tttaaatttg tcctacataa atgattacaa aggagcaata gatttgctta ataagtataa tttcaatgac gataaatata ttttattgaa ggcggaaatt cattataaaa atggtgatta tttaaaatct tatgaaaatt atttgaaatt gaagagtaaa tattttcaaa gcattgtttt tgatctaatt aggcttgcta tagaattaaa tattaaagaa gaggttttag agaacgctag atatttagtt gaaaagaatg ttgatttttc tgagagcatt tatcttgaga tctttgaatt cttagtaaca aggggagagc atgagtttgc tttaaatttt agctctcttt actttcctaa gtatattaat tcaagctttt cagacaaata tagttatttg ttgggaaaac tttatgagtc tgagagcaag cataaagatt ttttaaaggc tttgcattac tataaattgg ttattgataa ttaccctttt agttattatt atgagagagc caagataaga tatttatttt taaagcggtt tttttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat