SSGCID: Target Page

BobuA.19110.a

Uncharacterized protein (BB_0161)

CENTER ID:	BobuA.19110.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.19110.a.B2.GE39579	mature protein		14	533

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.19110.a.B2.PW37861	mature protein		14	533

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224326.49.peg.558
RefSeq:	NP_212295.2
UniProt:	O51183

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLFIIFFISA PLFSKEVYYR FADYSVNYSI VGKYEITKEE SEEEYGYKFT YDRNNNLILV DYIGKLSILM PSFFNASQIK IERSKRSERR IFLNRGLAFK NNNGVYIEHI EYMSDGRIKN IFNYNRSNEM VRDKYDVSYY HFLYDNEREF SVYRFNESGI QIKDLNNVYF TKISYDHTKF VKTVLYYDEN GYIVRSKNGT FGFRLTYDAN HNLIKEEYLD KSAYAVSNPF SVATKIYHYD VDGKVIEILN YDINNNLTPD ENNVAIYRYE YYFKDKDCYY KEYNYDNNNN LTISQNGYAM KKTIFYIQNN EKRIINYSNK INKNYNRDIP TVYEEKYEIM DNFKGIAIYS YKYDDNFYLI ENIFFDKNFN LIADVKGVMI YRYSYDKEGF LIAQEHFGGD FVNPIDDFEG VSKYKFSYDS NGNMISKKNY SKDGVLVADC NFVFEYLYEY DKQNRLISQK NFGSLGQLQD DIHGVSVYKY EYNKFGKISK QSNHGPDLRL RDDVGGFSIY KWIYDSRGNL IDFQKFDSLG NLI

NT Sequence

atgttattta taatattttt tattagtgct cctctttttt caaaagaagt ttactatagg tttgcagatt attctgttaa ttacagtata gtgggtaaat atgagattac aaaagaagag tctgaggaag agtacggata taaatttact tatgatcgca ataataatct tattttagtt gattatattg gtaagcttag tattttaatg ccttcttttt ttaatgcaag ccaaattaaa atagaaaggt ccaagcgcag cgaaaggcgc atatttctaa ataggggact tgcttttaaa aacaataatg gtgtttatat tgaacatata gagtatatga gtgatggcag gattaaaaat atttttaatt ataatagatc taatgaaatg gttagagata aatatgatgt ttcttattat cattttttat acgacaatga gagagagttt agtgtttaca gatttaatga atcagggatt caaatcaaag atttaaataa tgtgtatttt acaaagatca gctacgatca taccaaattt gttaaaacgg ttttgtatta cgatgagaat ggttatatcg taagatcaaa aaatggtact tttgggttta gattaactta tgatgctaat cataatctta ttaaagagga atatcttgat aaatcggctt atgctgtttc aaatccattt tctgtagcta ctaaaatata tcattatgat gttgatggaa aagttattga gattttaaat tatgatatta ataacaactt gactcctgat gaaaataatg tagcaattta tcgttatgaa tattatttta aagataaaga ttgctattac aaggaatata attacgacaa taataataat ttaacaatca gtcaaaatgg gtatgccatg aagaagacta ttttttatat ccaaaataat gaaaaaagaa taataaacta cagtaataag atcaataaaa actataatag agatattcct actgtttatg aggaaaagta tgaaataatg gataatttta aaggtattgc tatttatagt tataaatatg atgataattt ttatctaata gaaaatattt tttttgataa aaattttaat ttaatagctg atgttaaagg cgtgatgatt tatagatact cttacgacaa agaaggattt ttgattgctc aagagcattt tggtggggat tttgttaatc caatagatga ttttgaagga gtttcaaagt ataaatttag ttacgattct aatggcaata tgatttcaaa gaaaaattat tctaaggatg gagttttggt tgctgattgt aattttgttt ttgagtattt atatgaatat gataaacaaa ataggcttat ttctcaaaaa aattttggca gcttgggaca gttgcaggat gatattcatg gagttagtgt ttataaatat gaatataata aatttggaaa aatatcaaag cagtctaatc atggtccgga tttgagatta agagatgatg ttggaggatt ttcaatttat aagtggattt atgattctag aggcaattta atagattttc aaaaatttga ttctttgggt aatttaatct aa

Details for BobuA.19110.a.B2.GE39579

HARVESTED ON:	10/30/2015
SEQUENCED ON:	10/23/2015
EXPECTED MW:	63kDa
OBSERVED MW:	63kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHSK EVYYRFADYS VNYSIVGKYE ITKEESEEEY GYKFTYDRNN NLILVDYIGK LSILMPSFFN ASQIKIERSK RSERRIFLNR GLAFKNNNGV YIEHIEYMSD GRIKNIFNYN RSNEMVRDKY DVSYYHFLYD NEREFSVYRF NESGIQIKDL NNVYFTKISY DHTKFVKTVL YYDENGYIVR SKNGTFGFRL TYDANHNLIK EEYLDKSAYA VSNPFSVATK IYHYDVDGKV IEILNYDINN NLTPDENNVA IYRYEYYFKD KDCYYKEYNY DNNNNLTISQ NGYAMKKTIF YIQNNEKRII NYSNKINKNY NRDIPTVYEE KYEIMDNFKG IAIYSYKYDD NFYLIENIFF DKNFNLIADV KGVMIYRYSY DKEGFLIAQE HFGGDFVNPI DDFEGVSKYK FSYDSNGNMI SKKNYSKDGV LVADCNFVFE YLYEYDKQNR LISQKNFGSL GQLQDDIHGV SVYKYEYNKF GKISKQSNHG PDLRLRDDVG GFSIYKWIYD SRGNLIDFQK FDSLGNLI

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattcaaaag aagtttacta taggtttgca gattattctg ttaattacag tatagtgggt aaatatgaga ttacaaaaga agagtctgag gaagagtacg gatataaatt tacttatgat cgcaataata atcttatttt agttgattat attggtaagc ttagtatttt aatgccttct ttttttaatg caagccaaat taaaatagaa aggtccaagc gcagcgaaag gcgcatattt ctaaataggg gacttgcttt taaaaacaat aatggtgttt atattgaaca tatagagtat atgagtgatg gcaggattaa aaatattttt aattataata gatctaatga aatggttaga gataaatatg atgtttctta ttatcatttt ttatacgaca atgagagaga gtttagtgtt tacagattta atgaatcagg gattcaaatc aaagatttaa ataatgtgta ttttacaaag atcagctacg atcataccaa atttgttaaa acggttttgt attacgatga gaatggttat atcgtaagat caaaaaatgg tacttttggg tttagattaa cttatgatgc taatcataat cttattaaag aggaatatct tgataaatcg gcttatgctg tttcaaatcc attttctgta gctactaaaa tatatcatta tgatgttgat ggaaaagtta ttgagatttt aaattatgat attaataaca acttgactcc tgatgaaaat aatgtagcaa tttatcgtta tgaatattat tttaaagata aagattgcta ttacaaggaa tataattacg acaataataa taatttaaca atcagtcaaa atgggtatgc catgaagaag actatttttt atatccaaaa taatgaaaaa agaataataa actacagtaa taagatcaat aaaaactata atagagatat tcctactgtt tatgaggaaa agtatgaaat aatggataat tttaaaggta ttgctattta tagttataaa tatgatgata atttttatct aatagaaaat attttttttg ataaaaattt taatttaata gctgatgtta aaggcgtgat gatttataga tactcttacg acaaagaagg atttttgatt gctcaagagc attttggtgg ggattttgtt aatccaatag atgattttga aggagtttca aagtataaat ttagttacga ttctaatggc aatatgattt caaagaaaaa ttattctaag gatggagttt tggttgctga ttgtaatttt gtttttgagt atttatatga atatgataaa caaaataggc ttatttctca aaaaaatttt ggcagcttgg gacagttgca ggatgatatt catggagtta gtgtttataa atatgaatat aataaatttg gaaaaatatc aaagcagtct aatcatggtc cggatttgag attaagagat gatgttggag gattttcaat ttataagtgg atttatgatt ctagaggcaa tttaatagat tttcaaaaat ttgattcttt gggtaattta atctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat

Details for BobuA.19110.a.B2.PW37861

PURIFICATION DATe:	2/23/2016
CONCENTRATION:	12mg/ml
OBSERVED MW:	64kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	10
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTCAAAAG AAGTTTACTA TAGGTTTGCA GATTATTCTG TTAATTACAG TATAGTGGGT AAATATGAGA TTACAAAAGA AGAGTCTGAG GAAGAGTACG GATATAAATT TACTTATGAT CGCAATAATA ATCTTATTTT AGTTGATTAT ATTGGTAAGC TTAGTATTTT AATGCCTTCT TTTTTTAATG CAAGCCAAAT TAAAATAGAA AGGTCCAAGC GCAGCGAAAG GCGCATATTT CTAAATAGGG GACTTGCTTT TAAAAACAAT AATGGTGTTT ATATTGAACA TATAGAGTAT ATGAGTGATG GCAGGATTAA AAATATTTTT AATTATAATA GATCTAATGA AATGGTTAGA GATAAATATG ATGTTTCTTA TTATCATTTT TTATACGACA ATGAGAGAGA GTTTAGTGTT TACAGATTTA ATGAATCAGG GATTCAAATC AAAGATTTAA ATAATGTGTA TTTTACAAAG ATCAGCTACG ATCATACCAA ATTTGTTAAA ACGGTTTTGT ATTACGATGA GAATGGTTAT ATCGTAAGAT CAAAAAATGG TACTTTTGGG TTTAGATTAA CTTATGATGC TAATCATAAT CTTATTAAAG AGGAATATCT TGATAAATCG GCTTATGCTG TTTCAAATCC ATTTTCTGTA GCTACTAAAA TATATCATTA TGATGTTGAT GGAAAAGTTA TTGAGATTTT AAATTATGAT ATTAATAACA ACTTGACTCC TGATGAAAAT AATGTAGCAA TTTATCGTTA TGAATATTAT TTTAAAGATA AAGATTGCTA TTACAAGGAA TATAATTACG ACAATAATAA TAATTTAACA ATCAGTCAAA ATGGGTATGC CATGAAGAAG ACTATTTTTT ATATCCAAAA TAATGAAAAA AGAATAATAA ACTACAGTAA TAAGATCAAT AAAAACTATA ATAGAGATAT TCCTACTGTT TATGAGGAAA AGTATGAAAT AATGGATAAT TTTAAAGGTA TTGCTATTTA TAGTTATAAA TATGATGATA ATTTTTATCT AATAGAAAAT ATTTTTTTTG ATAAAAATTT TAATTTAATA GCTGATGTTA AAGGCGTGAT GATTTATAGA TACTCTTACG ACAAAGAAGG ATTTTTGATT GCTCAAGAGC ATTTTGGTGG GGATTTTGTT AATCCAATAG ATGATTTTGA AGGAGTTTCA AAGTATAAAT TTAGTTACGA TTCTAATGGC AATATGATTT CAAAGAAAAA TTATTCTAAG GATGGAGTTT TGGTTGCTGA TTGTAATTTT GTTTTTGAGT ATTTATATGA ATATGATAAA CAAAATAGGC TTATTTCTCA AAAAAATTTT GGCAGCTTGG GACAGTTGCA GGATGATATT CATGGAGTTA GTGTTTATAA ATATGAATAT AATAAATTTG GAAAAATATC AAAGCAGTCT AATCATGGTC CGGATTTGAG ATTAAGAGAT GATGTTGGAG GATTTTCAAT TTATAAGTGG ATTTATGATT CTAGAGGCAA TTTAATAGAT TTTCAAAAAT TTGATTCTTT GGGTAATTTA ATCTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT